Dermatology: Practical and Conceptual Research Letter | Dermatol Pract Concept. 2023;13(2):e2023169 1 Ultraviolet-Induced Fluorescence Dermatoscopy of Trichobacteriosis Axillaris Reveals Peripilar Yellow-Green Luminescent Concretions Marwan Al-Nasiri1, Cristian Navarrete-Dechent2,3, Katarzyna Korecka4,5, Natalia Salwowska5,6, Mohamad Goldust7, Paweł Pietkiewicz5,8 1 St. Michael’s Clinic, Shrewsbury, United Kingdom 2 Department of Dermatology, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile 3 Melanoma and Skin Cancer Unit, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile 4 Department of Skin Diseases, Regional Hospital, Poznań, Poland 5 Polish Dermatoscopy Group, Poznań, Poland 6 Department of Dermatology, School of Medicine, Medical University of Silesia, Katowice, Poland 7 Department of Dermatology, Yale School of Medicine, Yale University, New Haven, Connecticut, USA 8 Dermatology Private Practice, Poznań, Poland Key words: dermatoscopy, corynebacteria, trichomycosis, ultraviolet radiation, imaging Citation: Al-Nasiri M, Navarrete-Dechent C, Korecka K, Salwowska N, Goldust M, Pietkiewicz P. Ultraviolet-Induced Fluorescence Dermatoscopy of Trichobacteriosis Axillaris Reveals Peripilar Yellow-Green Luminescent Concretions. Dermatol Pract Concept. 2023;13(2):e2023169. DOI: https://doi.org/10.5826/dpc.1302a169 Accepted: February 4, 2023; Published: April 2023 Copyright: ©2023 Al-Nasiri et al. This is an open-access article distributed under the terms of the Creative Commons Attribution- NonCommercial License (BY-NC-4.0), https://creativecommons.org/licenses/by-nc/4.0/, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original authors and source are credited. Funding: None. Competing interests: None. Authorship: All authors have contributed significantly to this publication. Corresponding author: Paweł Pietkiewicz, Centrum Medyczne Zwierzyniecka, Zwierzyniecka Street 30/28, Poznań 60-814. Telephone: +48 602 133 503 Email: pietkiewicz.pp@gmail.com Introduction Trichobacteriosis axillaris (TA) is a superficial bacterial in- fection caused by Corynebacterium species that commonly affect the soft keratin layer around the axillary hair and, to a lesser extent, pubic, genital, and rarely scalp hair [1]. In most cases, the diagnosis is supported by the patient’s history of smelly/sweaty armpits, in addition to the most common clini- cal appearance of irregular yellow cement-like insoluble con- cretions wrapped around the hair shaft predominantly caused by Corynebacterium flavescens. Micrococcus castelani or co-infection of Corynebacterium sp. and Serratia mascescens have been cultured from rare red variant, and Micrococus nigricans from the rare black concretions [1]. The disorder is more common in tropical and humid climates, and usually affects teenagers and young adults. TA can be differentiated from piedra, pediculosis, hair casts and Trichosporon aselie infections with Wood’s lamp (peak wavelength 365 nm) [1,2]. However, when atypical hair bearing areas are affected, dermatoscopy can serve as a helpful diagnostic aid by demonstrating these typical yellow concretions [3]. Ultravi- olet-induced fluorescence dermatoscopy (UVFD), a novel di- agnostic technique, can evoke neon-like “UV fluorescence”. The chemical compound excited with UV irradiation emits 2 Research Letter | Dermatol Pract Concept. 2023;13(2):e2023169 new (usually longer) wavelength, that can belong to the vis- ible light spectrum [4]. To the best of our knowledge, our report is the first report on the use of UVFD in TA. Case Presentation A 62-year-old Caucasian male presented for a routine skin check. The clinical examination of the axillary hair revealed asymptomatic thin irregular creamy yellow concretions en- circling several hair shafts (Figure 1A). The detail of these concretions was more recognizable in adequate lighting and close up imaging (Figure 1B). In this case, additional non-contact UVFD (DL5, Dermlite; ×10 magnification) showed distinctive bright yellow-green excited luminescence (Figure 1C), which enabled a spot diagnosis of TA. The patient was advised to shave axillary hair, treated with 2% topical fucidic acid, and discharged without further testing. Conclusions The diagnosis of TA is usually not clinically challenging. However, diagnostic difficulties arise in atypical clinical presentations or atypical locations [3]. UVFD can be used to further increase the diagnostic confidence by visualizing yellow-green luminescent conglomerates adhering to the hair shafts. This adequately rules out other clinical differential diagnoses that do not exhibit this unique feature. Therefore, no cost-incurring and time-consuming investigations are re- quired. The exact source of the yellow-green luminescence has not been identified yet, but it is hypothesized to originate from the admixture of the sweat and bacterial products. The utilization of UVFD, initially used for tumor margin delinea- tion in melanoma, is expected to be further utilized in the di- agnosis of multiple neoplastic, inflammatory, and infectious conditions of the skin and adnexa. References 1. Bonifaz A, Váquez-González D, Fierro L, Araiza J, Ponce RM. Trichomycosis (trichobacteriosis): clinical and microbiological experience with 56 cases. Int J Trichology. 2013;5(1):12-16. DOI: 10.4103/0974-7753.114704. PMID: 23960390. PMCID: PMC3746219. 2. Dyer JM, Foy VM. Revealing The Unseen: A Review of Wood’s Lamp in Dermatology. J Clin Aesthet Dermatol. 2022 Jun;15(6):25-30. PMID: 35783566. PMCID: PMC9239119. 3. Navarrete-Dechent C, Fich F, Gonzalez S. Trichomycosis (trichobacteriosis) capitis misdiagnosed as poliosis: the utility of dermoscopy and why it should always be done. J Eur Acad Dermatol Venereol. 2017;31(6):e275-e276. DOI: 10.1111/jdv .14013. PMID: 27740739. 4. Mojeski JA, Almashali M, Jowdy P, et al. Ultraviolet imaging in dermatology. Photodiagnosis Photodyn Ther. 2020;30:101743. DOI: 10.1016/j.pdpdt.2020.101743. PMID: 32198019. Figure 1. Trichobacteriosis axillaris. (A) Clinical presentation with creamy whitish segmentally “thickened” hair of the axilla. (B) Non-contact polarized dermatoscopy showing yellowish-whitish bacterial conglomerates wrapping the hair. (C) Non-contact ul- traviolet-induced fluorescence dermatoscopy featuring prominent yellowish-greenish luminescence confirming the diagnosis. (Xiaomi Mi 10T Pro 5G, Xiaomi, paired with DL5, Dermlite; ×10 magnification).