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Introduction Based on the latest World Health Organization (WHO) esti- mates of December 2016, there were 212 million cases of malaria in 2015 and 429 000 deaths, with sub-Saharan Africa accounting for 90% of all malaria deaths in the world.1 This staggering number of deaths resulting from malaria is associ- ated with the continuous spread of Plasmodium falciparum resistance to available antimalarial drugs, posing severe threats to existing malaria control programs. The lack of a deployable malaria vaccine coupled with increasing cases of drug-resistant infections makes the search for new antimalarials imperative.2,3 Owing to the high cost of malaria treatment in developing countries in general and Nigeria in particular, many have resulted to consulting traditional herbalists or the use of medic- inal plants for treatment of infection.4 For thousands of years, in various parts of the world, tradi- tional herbal medicines have been used to treat malaria.5–9 Two of the most important antimalarial drugs in use, artemisinin and quinine, were either derived directly from plants or synthe- sized using chemical structures of plant-derived compounds as templates.5,9,10 Most of the people in malaria endemic coun- tries still depend on traditional herbal remedies, which could be linked to limited availability as well as unaffordability of existing pharmaceutical drugs.11 Blighia sapida, also referred to as ackee or ackee apple, is a medicinal plant of the family Sapindaceae that naturally exists in the forests of most West African countries, where the fruits are used for several purposes, but rarely eaten.12 When mixed with water, the green fruits of this plant produce lather and are used for laundry, in addition to its seeds being used for making soap due to its high oil content.13 Some parts of this plant also serve medicinal purposes, such as the juice from the leaf is tra- ditionally used to treat conjunctivitis,12 whereas its twiggy leaves, once beaten into a pulp, are applied to the forehead for treating migraine or headaches.14 For over 20 years, the Centre for Scientific Research into Plant Medicine (CSRPM) in Ghana has used this plant for the treatment of diarrhea.15 The antidiarrheal activity of the stem bark of B sapida has also been reported,16 including reports on its traditional use for the treat- ment of dysentery, yellow fever, burns, wounds, or conjunctivi- tis, etc.14 Murine experiments have also demonstrated its utility in treating pancreatic β-cell dysfunction,17 elevated blood glu- cose, dyslipidemia, and oxidative stress in alloxan-induced dia- betic rats.18 In view of the significant challenges imposed by drug resist- ance, traditional plant extracts continue to be promising sources of new antimalarial compounds.9 The use of traditional medi- cines for the treatment of malaria and other diseases continues to be a growing practice among many African families, despite the availability of orthodox method of treatment.19 In addition, the short supply of artemisinin compounds leading to alternative Ethanol Extract of Blighia sapida Stem Bark Show Remarkable Prophylactic Activity in Experimental Plasmodium berghei–Infected Mice Olayinka O Otegbade1, Johnson A Ojo1, Dolapo I Adefokun2, Oyindamola O Abiodun3, Bolaji N Thomas4 and Olusola Ojurongbe1 1Department of Medical Microbiology & Parasitology, Ladoke Akintola University of Technology, Osogbo, Nigeria. 2Department of Pharmacology & Therapeutics, Ladoke Akintola University of Technology, Osogbo, Nigeria. 3Department of Pharmacology & Therapeutics, University of Ibadan, Ibadan, Nigeria. 4Department of Biomedical Sciences, College of Health Sciences and Technology, Rochester Institute of Technology, Rochester, NY, USA. ABSTRACT: This work explores the antiplasmodial potential of ethanol extract of Blighia sapida (Lin. Sapindaceae) in chloroquine (CQ)- resistant Plasmodium berghei (ANKA strain)–infected mice. Chloroquine-resistant (ANKA) strain of P berghei was inoculated intraperitoneally into Swiss albino mice. Mice were treated orally for 4 consecutive days, before and after inoculation (prophylactic, suppressive, and curative models) with graded doses of the plant extracts with Artemether-Lumefantrine (Coartem) as control. Prophylactically, the extract showed a remarkable activity in the chemosuppression of P berghei parasites (P < .01) ranging from 57% to 36.5% at doses of 200 to 800 mg/kg, respectively, whereas Coartem (10 mg/kg) produced 62.1% chemosuppression. No significant chemosuppression was observed in the curative and suppressive models. The plant extract appeared to be safe at the highest dose tested (5000 mg/kg) for acute toxicity, with no adverse effect on the different organs. The plant extract possesses prophylactic antimalarial activity, which supports its use in the prevention of malaria. KEywoRdS: Plasmodium berghei, Blighia sapida, chemosuppression, curative, prophylaxis, efficacy and safety RECEIVEd: June 7, 2017. ACCEPTEd: August 8, 2017. PEER REVIEw: Two peer reviewers contributed to the peer review report. Reviewers’ reports totaled 428 words, excluding any confidential comments to the academic editor. TyPE: Original Research FundIng: The author(s) received no financial support for the research, authorship, and/or publication of this article. dEClARATIon oF ConFlICTIng InTERESTS: The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. CoRRESPondIng AuTHoR: Olusola Ojurongbe, Department of Medical Microbiology & Parasitology, Ladoke Akintola University of Technology, PMB, 4400 Osogbo, Nigeria. Email: oojurongbe@lautech.edu.ng 728725DTI0010.1177/1177392817728725Drug Target InsightsOtegbade et al research-article2017 https://uk.sagepub.com/en-gb/journals-permissions mailto:oojurongbe@lautech.edu.ng 2 Drug Target Insights production methods,9 as well as the desire for low-cost drug delivery strategies,7 has led to significant research efforts to deci- pher alternative plant products that could serve as effective anti- malarials, especially in sub-Saharan Africa. In this report, the antiplasmodial effect of ethanolic extract of B sapida stem bark in vivo is reported, using the suppressive, curative, and prophylactic model. Materials and Methods Plant collection, authentication, and extract preparation The stem bark of B sapida was collected in December 2014 from Ipetumodu in the South West of Nigeria. Plant material was identified in the herbarium of the Botany Department of Obafemi Awolowo University, Ile-Ife, Osun State Nigeria with voucher number IFE-17629 by Mr GA Ademoriyo. The stem bark was air-dried at room temperature and powdered. About 50 g of the powdered plant was macerated in 500 mL of 99% methanol (1:10 W/V ) for 48 hours and then filtered. With the aid of a rotatory evaporator, filtrate was concentrated to dry- ness and the residue obtained was stored until future use. Ethical consideration Experimental procedures and protocols used in this study were in conformity with National Institute of Health’s recommen- dations in guide for the care and use of laboratory animals and in accordance with the principles of Helsinki Declaration. Parasite/infection Chloroquine-resistant P berghei ANKA, obtained from Institute for Medical Research and Training (IMRAT), University College Hospital, Ibadan, Nigeria, was used to eval- uate the antimalarial activity of B sapida in this study. Groups of Swiss albino mice (5 per cage) were infected intraperito- neally with 1 × 107 parasitized erythrocytes from an infected donor mouse. The day of infection was defined as day 0 (D0) and subsequent days D1, D2, etc. Antimalarial medication Standard Coartem (Artemether and Lumefantrine), used for treating malaria (Mekophar Chemical Pharmaceutical Joint- Stock Co, Ho Chi Minh City, Vietnam) and obtained from a pharmacy, was used as reference for the antimalarial screening in this study. Acute toxicity test (assessment of minimum lethal dose) The assessment of minimum lethal dose was done in 2 phases, following published methods.20 In phase 1, groups of 3 mice in each group were given oral doses of 10, 100, and 1000 mg/kg body weight of B sapida ethanolic extract, respectively, and observed for 24 hours for mortality. In phase 2, 3 mice in each group groups were orally administered with 1600, 2900, and 5000 mg/kg body weight of the extract, respectively, and moni- tored for 24 hours, observing for mortality. The phase 2 study was conducted because we observed no deaths among animals in phase 1 study. The lethal dose and the penultimate dose to the lethal dose would indicate the value of the LD50.21,22 In vivo antiplasmodial determination Swiss albino mice (weight: 18-25 g), obtained from the Animal House, Department of Pharmacology, University of Ibadan, kept according to Institutional Animal Care and Use Committee (IACUC) standards, were allowed to acclimatize to the new environment for a week before study initiation. Each mouse was inoculated with 0.2 mL of infected blood containing about 1 × 107 dose of P berghei berghei (about 16.6%) from a donor mouse. Each mouse was inoculated on day 0 (D0) (intraperitoneally) for the suppressive and cura- tive model and on the fifth day (D4) for the prophylactic model. Suppressive test (4-day test) The 4-day suppressive test was conducted according to the method of Peters (1975). In this, 25 mice distributed into 5 groups of 5 mice each were infected with 1 × 107 of parasitized red blood cells on day 0. Animals in group 1 received normal saline, those in groups 2 to 4 received 200, 400, and 800 mg/kg of ethanol extract of B sapida test extract, whereas those in group 5 (positive control) received 10 mg/kg of the standard drug Coartem. Treatment was administered orally and contin- ued daily for the next 3 days for the group that received Coartem or 4 consecutive days for extract-treated group. On day 4, blood samples were collected from the caudal vein and stained with 10% Giemsa stain. Thereafter, the number of the parasitized cells was estimated under the microscope using ×100 objective.23 The numbers of infected erythrocytes were counted until 1000 erythrocytes were achieved. The average percentage suppression of parasitemia was calculated in com- parison with controls as follows: % % % Suppression parasitemia in negative control parasitemia in t = _ eest group parasitemia in negative control% 100× Prophylactic test The prophylactic activity of the extract was performed as described previously.24 In this, Swiss albino mice were rand- omized into 5 groups of 5 mice each: group 1 was treated with normal saline only, groups 2 to 4 were treated with 200, 400, and 800 mg/kg/d of the ethanol extract of B sapida, and group Otegbade et al 3 5 served as positive control (treated with 10 mg/kg/d of the standard drug Coartem). Treatment started on day 0 and con- tinued for 4 consecutive days, by which mice were inoculated intraperitoneally with 1 × 107 of the parasite. On day 7 (72 hours after inoculation), blood smears made from each mouse were stained with Giemsa, number of the parasitized cells was estimated, and the percentage suppression was evaluated. Curative test This was also performed with standard protocol,24 as previ- ously described. Twenty-five mice distributed into 5 groups of 5 were infected intraperitoneally on the first day (day 0) with 0.2 mL of parasite inoculum. After 72 hours of infection, all animals were treated with different regimens: group 1 received normal saline only; groups 2 to 4 received 200, 400, and 800 mg/ kg body weight of plant extract, respectively; whereas group 5 received 10 mg/kg body weight of the standard antimalarial drug (Coartem). Treatment was continued for 5 consecutive days, blood smears were made and microscopically examined to monitor the degree of parasitemia as well as estimating the number of parasitized cells. Histologic procedures These were performed with hematoxylin and eosin procedures.25 Briefly, organs such as liver, spleen, kidney, and testes from each animal were fixed in 10% formal saline, grossed and cut longitu- dinally into pieces of 4 mm in thickness, and processed for histo- logic study using standard procedure. Microtome sections were dried on a hot plate and later stained with hematoxylin and eosin stains for examination with a light microscope. Data analysis Percentage parasitemia and mean chemosuppression were determined using 1-way analysis of variance; Student- Newman-Keuls test was used for analysis and comparing of results at 95% confidence interval; P < .05 was set for statistical significance. Results About 11.10 g of brown crude extract was obtained from the extraction procedure, a percentage yield of 22%. The acute tox- icity test revealed that the extract appeared to be nontoxic at the highest dose (5000 mg/kg) tested. No toxic symptoms or mortality was observed in any of the 3 animals in 3 groups both in phase 1 and phase 2 acute toxicity test study. All animals were still alive at the end of the acute toxicity test, with LD50 value greater than 5000 mg/kg body weight, showing the extract to be nontoxic. Histology sections of the liver, spleen, kidney, and testes reveal normal tissue morphology at all doses, except at 5000 mg/kg (black ring), where we observed a mild perivascular infiltration of liver inflammatory cells (Figure 1); otherwise, hepatocytes appeared normal. Suppressive effect of B sapida ethanolic extract on P berghei In the suppressive test, mean parasitemia on day 4 after infec- tion ranged from 14.20 ± 1.17 to 18.96 ± 0.60 for 200 and 800 mg/kg, respectively (Figure 2). The value for control was 19.02 ± 1.20, whereas for Coartem was 8.38 ± 0.39. The percent- age chemosuppression of the extract ranged from 6.8% to 0.3% for 200 and 800 mg/kg, respectively, whereas that for standard antimalarial Coartem was 55.9%. Percentage chemosuppression showed increases with decrease in dose levels of the extract, indicating that maximal chemosuppression could be reached at the lowest dose, although intangible. Percentage chemosuppres- sion in Coartem was very much higher than in control and all the doses of the extract, revealing that extract had little or no suppressive effect at all doses. Percentage parasitemia in control, compared with 400 and 800 mg/kg, was not statistically signifi- cant (P > .05), whereas that of 200 mg/kg compared with con- trol, 400 and 800 mg/kg, was statistically significant (P < .01) (Figure 2). Percentage parasitemia in Coartem was much lower than in extract at the highest dose, showing statistical signifi- cance (P < .001), but percentage parasitemia of the lowest dose of the extract was slightly higher in comparison with Coartem, with the difference not significant (Figure 2). Prophylactic effect of B sapida ethanolic extract on P berghei The percentage parasitemia in mice infected and treated with 200, 400, and 800 mg/kg was 8.22 ± 0.32, 9.7 ± 0.31, and 12.12 ± 0.54, respectively (Figure 3). Untreated saline group had percentage parasitemia of 19.10 ± 0.33, whereas that of Coartem-treated positive control was 7.24 ± 0.91. Percentage chemosuppression of parasites in mice treated with 200, 400, Figure 1. Photomicrographs of liver and kidney at the end of acute toxicity assessment. (A) Normal liver morphology. (B) (Black ring) mild perivascular infiltration of the liver inflammatory cells after treatment with 5000 mg/kg of the extract. (C) Normal kidney morphology. (D) Kidney remains normal after treatment with 5000 mg/kg of the extract. Haematoxylin and Eosin, 400x original magnification. 4 Drug Target Insights and 800 mg/kg was 57%, 50.8%, and 36.5%, respectively, whereas that of Coartem was 62.1%. Unlike our observation in the suppressive model, percentage parasitemia decreased with a decrease in extract dose levels, showing that a tangible optimal Figure 2. Suppressive effect of methanol extract of bark of Blighia sapida on Plasmodium berghei. Bars are expressed as mean ± SEM (n = 5); ***P < .001, Coartem versus normal saline (NS); **P < .01, 200 mg/kg versus normal saline. Figure 3. Prophylactic effect of ethanol extract of Blighia sapida on Plasmodium berghei in mice. Bars are expressed as mean ± SEM (n = 5); ***P < .001, test and control versus normal saline (NS). Otegbade et al 5 chemosuppression can be obtained at the lowest dose in this model and is statistically significant at all doses when compared with nontreated group (P < .001; Figure 3). The percentage of the extract at the lowest dose (200 mg/kg) was comparable with that of Coartem, although Coartem exhibited a highly signifi- cant (P < .001) decrease in percentage parasitemia compared with negative control and 800 mg/kg. Curative effect of B sapida ethanolic extract on P berghei Figure 4 shows curative effect of the extract at different graded doses. Unlike the suppressive and prophylactic models, none of the extract doses tested showed significant activity. The lowest dose (200 mg/kg) that had shown significant activity in other models showed no activity in our model. For day 3, the values for Coartem versus control versus 200 mg/kg of the extract was 1.74 ± 0.29 versus 7.58 ± 0.44 versus 7.18 ± 0.82, with similar findings on day 7 (21.48 ± 0.68, 17.00 ± 0.58, and 2.06 ± 0.39 for control), 200 mg/kg of extract and Coartem, respectively, revealing the extract had no significant activity against the parasite when compared with control. Effect of malaria on weight of malaria-induced mice The weights of all test animals decreased 24 hours after infec- tion, with this decrease continuing among the suppressive, curative, and untreated groups 72 hours after treatment. However, by day 5 (21.40 ± 1.20), this progressive weight loss was turned around for the prophylactic group when compared with the weights obtained at the same dose on day 0 (20.78 ± 0.92) and day 7 (21.70 ± 1.08) (Table 1). Effect of B sapida ethanolic extract on organs of P berghei–infected mice The kidney, liver, and spleen of the mice revealed normal tissue architecture after treatment, demonstrating that this extract appears safe on the major organs of the mice (slides not shown). Figure 4. Curative effect of ethanol extract of Blighia sapida on Plasmodium berghei in mice. NS indicates normal saline. Table 1. Weights of mice for prophylactic model. DOSE, Mg/Kg DAy 0 DAy 1 DAy 2 DAy 3 DAy 4 DAy 5 DAy 6 DAy 7 NS 21.40 ± 1.02 21.85 ± 0.99 22.08 ± 1.0 21.94 ± 0.96 22.28 ± 1.10 21.90 ± 0.93 19.45 ± 0.83 17.02 ± 0.75 200 20.78 ± 0.92 20.16 ± 0.94 21.02 ± 1.02 20.24 ± 1.10 20.96 ± 1.03 21.40 ± 1.20 20.16 ± 1.00 21.70 ± 1.08 400 17.28 ± 0.44 17.20 ± 0.39 17.46 ± 0.54 17.36 ± 0.51 17.62 ± 0.41 18.00 ± 0.47 17.32 ± 0.43 16.96 ± 0.40 800 22.28 ± 1.21 22.20 ± 1.40 22.50 ± 1.47 21.64 ± 1.33 22.12 ± 1.34 22.80 ± 1.52 21.90 ± 1.36 22.68 ± 1.44 Coartem 19.58 ± 1.00 19.88 ± 0.90 20.14 ± 0.78 19.78 ± 0.70 19.58 ± 0.70 20.12 ± 0.75 19.52 ± 0.63 20.42 ± 0.51 Abbreviation: NS, normal saline. The baseline weights were taken before inoculation and subsequent weights taken before, during and after treatments. Data are expressed as mean ± SEM. Values do not indicate statistical significance to one another. 6 Drug Target Insights Survival time of mice in the 3 models The survival time for mice in the prophylactic group was longer than those in suppressive and curative groups (Figure 5). Mice in prophylactic group started dying on day 9, whereas most were still alive at day 20 and beyond. However, animals in cura- tive group started dying on day 5 and were all dead by day 9, whereas those in the suppressive group started dying on day 5 with 98% dead by day 11. These results clearly demonstrate the prophylactic effect of this extract, at different doses, on malaria- infected animal. Discussion Despite the many and varying efforts at malaria control, this disease is still a global threat of enormous proportion and a significant contributor to health and economic inequities in endemic countries. Inhabitants of these countries, under the scourge of disease, face the conundrum of finding appropriate, effective, and cheap means to protect themselves against infec- tion, whereas the parasite, however, has shown an increasing resistance to available antimalarial drugs. This resistance is also favored by population movement and human migration lead- ing to the introduction of resistant parasite to areas previously free of drug resistance. The possibility of synthesizing new antimalarials from plants has become a major policy goal in the control arena, becoming more urgent, in light of the limited number of antimalarials in development, the potential of para- site resistance to the only available therapeutic drugs (arte- misinin and its derivatives), and the poor solubility of artemisinin. These are some of the factors driving the design of alternative methods of artemisinin delivery that could poten- tially slow the process of resistance evolution.10,26,27 We dem- onstrate that B sapida investigated in this study possesses antimalarial properties that deserve further analysis and could become a cheap, readily available antimalarial in developing countries. This study shows that B sapida has remarkable activity (almost equivalent with Coartem), when used as a prophylac- tic agent in chloroquine-resistant P berghei–infected mice, with the 7-day assay leading to a significant parasitemia sup- pression. Maximum activity was recorded at the lowest dose (200 mg/kg), with a decrease with increasing dosage, implying that the extract had no pronounced activity at higher doses, and that the small dose suffices to produce significant antima- larial activity. This observation concurs with previous result, where the minimum inhibitory activity shown by the metha- nolic extract of B sapida against Staphylococcus aureus started at a dose of 200 mg/mL, but further increase in activity was seen as the dose was decreased to 100 mg/mL, suggesting that the lower the dose, the higher the activity displayed.28 It is worth noting that B sapida extract exhibited the most active antiplas- modial properties at the lowest dosage tested. This dose- dependent activity potentially shows that response at low dose is more effective than response inhibition at high dose.29 Our results show that B sapida extracts will serve as excellent anti- malarial agent in the prophylactic model of treatment. The absence of death after administering 5000 mg/kg body weight of B sapida extract in the acute toxicity test suggests that the extract appears to be nontoxic, an observation supported by the toxicity scale principle, which states that any chemical showing an LD50 greater than 5000 mg/kg is practically non- toxic.30 In this report, the acute toxicity test conducted with the extracts demonstrate their safety because the highest dose caused no death. Interestingly, the highest dose used to treat parasite-infected mice from which antimalarial activity was elicited was much lower than the highest acute dose, thus mak- ing the extract quite selective and safe. Loss of appetite, ultimately leading to weight loss, is one of the characteristics of malaria infection.31 We observed weight loss in P berghei–infected animals after infection, with the ini- tial weight loss recorded reversed after 5 days of treatment with the extracts in the prophylactic group, but not in the curative and suppressive groups, which showed progressive weight loss. This result in the suppressive and curative groups agrees with earlier report of continuous weight loss among treated animals compared with nontreated animals32 but disagrees with our previous study using extracts from Russelia equisetiformis, which showed a reversal in body weight after treatment.3 However, the prophylactic group in this study where we observed slight increase after treatment agrees with our previous study. The survival time for mice in the prophylactic group extended beyond day 20, whereas those in the curative model did not survive beyond day 9. About 98% of the mice in the suppressive model were dead by day 15, with only 2% surviving till day 20 but not beyond, further confirming the prophylactic activity of B sapida ethanol extract. Although there have been some reports of B sapida poison- ing in people who consumed the fruit (ackee) in particular,33–37 such poisonous effect was not observed with the stem bark used in our study. Histologic examination of the liver, kidney, and spleen showed no toxicity to the organs after treatment with the at all doses used, agreeing with the results Figure 5. graph showing survival time of mice in the 3 models. Otegbade et al 7 of a different study using Blighia unijugata, which showed no toxicity to the liver and kidney when administered in a dose- dependent fashion to Wistar rats more than 4 weeks.38 Histology of the testes reveals that extract has no toxic effect on male animals and as such not inhibitory to fertility. The dif- ference in the reported toxicity may be due to the part of the plant examined. The seed is toxic, whereas the stem bark appears safe. Conclusively, our results suggest that the ethanol bark extract of B sapida show some intrinsic antimalarial activities by its prophylactic ability against chloroquine-resistant P berghei parasites. This performance can surely be improved on in future studies if the crude extract is purified and the active substituents are identified and the doses further fractionated. The extracts have considerably low toxicities in experimental mice, this result supporting the traditional use of this plant for the prophylactic treatment of malaria only. Acknowledgements The authors are grateful to Dr GO Gbotosho, Malaria Research Laboratory, Institute for Medical Research and Training (IMRAT), University College Hospital, Ibadan, Nigeria, for providing the malaria parasite used for the study, also to Mrs Sakirat Braimah for technical assistance. Author Contributions OO conceived and designed the experiments. OOO, JAO, and DIA performed the laboratory work. JAO and DIA analyzed data. OOO wrote first draft of manuscript. JAO, DIA, and BNT contributed to writing of manuscript. BNT, OOA, and OO made critical revisions and approved final version. All authors reviewed and approved the final manuscript. Disclosures and Ethics The authors also confirmed that this article is unique and not under consideration or published in any other journal. Experimental procedures and protocols are in conformity with National Institute of Health’s recommendations in guide for the care and use of laboratory animals and the principles of the Declaration of Helsinki. R EfER EnCEs 1. WHO. WHO—World Malaria Report 2015. WHO; http://www.who.int/malar- ia/publications/world-malaria-report-2015/report/en/. Published 2015. Accessed February 5, 2017. 2. Ogungbamigbe TO, Ojurongbe O, Ogunro PS, et al. Chloroquine resistant Plasmodium falciparum malaria in Osogbo Nigeria: efficacy of amodiaquine + sul- fadoxine-pyrimethamine and chloroquine + chlorpheniramine for treatment. Mem Inst Oswaldo Cruz. 2008;103:79–84. 3. Ojurongbe O, Ojo JA, Adefokun DI, Abiodun OO, Odewale G, Awe EO. In vivo antimalarial activities of Russelia equisetiformis in Plasmodium berghei infect- ed mice. Indian J Pharm Sci. 2015;77:504–510. 4. Awe EO, Adeloye A, Idowu T, Olajide OA, Makinde J. Antinociceptive effect of Russelia equisetiformis leave extracts: identification of its active constituents. Phytomedicine. 2008;15:301–305. 5. Basco LK, Mitaku S, Skaltsounis AL, et al. In vitro activities of furoquinoline and acridone alkaloids against Plasmodium falciparum. Antimicrob Agents Chemother. 1994;38:1169–1171. 6. Ekué MRM, Sinsin B, Eyog-Matig O, Finkeldey R. Uses, traditional manage- ment, perception of variation and preferences in ackee (Blighia sapida K.D. 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