id sid tid token lemma pos dti-1354 1 1 31drug 31drug NUM dti-1354 1 2 target target NOUN dti-1354 1 3 insights insight VERB dti-1354 1 4 2014:8 2014:8 NUM dti-1354 1 5 open open ADJ dti-1354 1 6 access access NOUN dti-1354 1 7 : : PUNCT dti-1354 1 8 full full ADJ dti-1354 1 9 open open ADJ dti-1354 1 10 access access NOUN dti-1354 1 11 to to ADP dti-1354 1 12 this this PRON dti-1354 1 13 and and CCONJ dti-1354 1 14 thousands thousand NOUN dti-1354 1 15 of of ADP dti-1354 1 16 other other ADJ dti-1354 1 17 papers paper NOUN dti-1354 1 18 at at ADP dti-1354 1 19 http://www.la-press.com http://www.la-press.com X dti-1354 1 20 . . PUNCT dti-1354 2 1 drug drug NOUN dti-1354 2 2 target target NOUN dti-1354 2 3 insights insight NOUN dti-1354 2 4 molecular molecular ADJ dti-1354 2 5 and and CCONJ dti-1354 2 6 kinetic kinetic ADJ dti-1354 2 7 characterization characterization NOUN dti-1354 2 8 of of ADP dti-1354 2 9 babesia babesia NOUN dti-1354 2 10 microti microti PROPN dti-1354 2 11 gray gray ADJ dti-1354 2 12 strain strain NOUN dti-1354 2 13 lactate lactate NOUN dti-1354 2 14 dehydrogenase dehydrogenase NOUN dti-1354 2 15 as as ADP dti-1354 2 16 a a DET dti-1354 2 17 potential potential ADJ dti-1354 2 18 drug drug NOUN dti-1354 2 19 target target NOUN dti-1354 2 20 patrick patrick PROPN dti-1354 2 21 vudriko1,2 vudriko1,2 PROPN dti-1354 2 22 , , PUNCT dti-1354 2 23 tatsunori tatsunori PROPN dti-1354 2 24 masatani1 masatani1 PROPN dti-1354 2 25 , , PUNCT dti-1354 2 26 shinuo shinuo ADJ dti-1354 2 27 cao1 cao1 PROPN dti-1354 2 28 , , PUNCT dti-1354 2 29 mohamad mohamad PROPN dti-1354 2 30 alla alla PROPN dti-1354 2 31 terkawi1 terkawi1 PROPN dti-1354 2 32 , , PUNCT dti-1354 2 33 ketsarin ketsarin NOUN dti-1354 2 34 kamyingkird1 kamyingkird1 PROPN dti-1354 2 35 , , PUNCT dti-1354 2 36 ahmed ahmed PROPN dti-1354 2 37 a. a. PROPN dti-1354 2 38 mousa1 mousa1 PROPN dti-1354 2 39 , , PUNCT dti-1354 2 40 paul paul PROPN dti-1354 2 41 f. f. PROPN dti-1354 2 42 adjou adjou VERB dti-1354 2 43 moumouni1 moumouni1 PROPN dti-1354 2 44 , , PUNCT dti-1354 2 45 yoshifumi yoshifumi ADJ dti-1354 2 46 nishikawa1 nishikawa1 NOUN dti-1354 2 47 and and CCONJ dti-1354 2 48 xuenan xuenan VERB dti-1354 2 49 xuan1 xuan1 PROPN dti-1354 3 1 1national 1national ADJ dti-1354 3 2 research research NOUN dti-1354 3 3 center center NOUN dti-1354 3 4 for for ADP dti-1354 3 5 protozoan protozoan NOUN dti-1354 3 6 diseases disease NOUN dti-1354 3 7 ( ( PUNCT dti-1354 3 8 nrcpd nrcpd PROPN dti-1354 3 9 ) ) PUNCT dti-1354 3 10 , , PUNCT dti-1354 3 11 obihiro obihiro PROPN dti-1354 3 12 university university PROPN dti-1354 3 13 of of ADP dti-1354 3 14 agriculture agriculture NOUN dti-1354 3 15 and and CCONJ dti-1354 3 16 veterinary veterinary ADJ dti-1354 3 17 medicine medicine NOUN dti-1354 3 18 , , PUNCT dti-1354 3 19 inada inada PROPN dti-1354 3 20 - - PUNCT dti-1354 3 21 cho cho PROPN dti-1354 3 22 , , PUNCT dti-1354 3 23 obihiro obihiro PROPN dti-1354 3 24 , , PUNCT dti-1354 3 25 hokkaido hokkaido PROPN dti-1354 3 26 , , PUNCT dti-1354 3 27 japan japan PROPN dti-1354 3 28 . . PUNCT dti-1354 4 1 2department 2department NUM dti-1354 4 2 of of ADP dti-1354 4 3 veterinary veterinary ADJ dti-1354 4 4 pharmacy pharmacy NOUN dti-1354 4 5 , , PUNCT dti-1354 4 6 clinics clinic NOUN dti-1354 4 7 and and CCONJ dti-1354 4 8 comparative comparative ADJ dti-1354 4 9 medicine medicine NOUN dti-1354 4 10 , , PUNCT dti-1354 4 11 college college NOUN dti-1354 4 12 of of ADP dti-1354 4 13 veterinary veterinary ADJ dti-1354 4 14 medicine medicine NOUN dti-1354 4 15 , , PUNCT dti-1354 4 16 animal animal NOUN dti-1354 4 17 resources resource NOUN dti-1354 4 18 and and CCONJ dti-1354 4 19 biosecurity biosecurity NOUN dti-1354 4 20 , , PUNCT dti-1354 4 21 makerere makerere NOUN dti-1354 4 22 university university NOUN dti-1354 4 23 , , PUNCT dti-1354 4 24 kampala kampala PROPN dti-1354 4 25 , , PUNCT dti-1354 4 26 uganda uganda PROPN dti-1354 4 27 . . PUNCT dti-1354 5 1 abstr abstr PROPN dti-1354 5 2 act act PROPN dti-1354 5 3 : : PUNCT dti-1354 6 1 babesia babesia NOUN dti-1354 6 2 microti microti PROPN dti-1354 6 3 is be AUX dti-1354 6 4 an an DET dti-1354 6 5 emerging emerge VERB dti-1354 6 6 zoonotic zoonotic ADJ dti-1354 6 7 protozoan protozoan NOUN dti-1354 6 8 organism organism NOUN dti-1354 6 9 that that PRON dti-1354 6 10 causes cause VERB dti-1354 6 11 “ " PUNCT dti-1354 6 12 malaria malaria NOUN dti-1354 6 13 - - PUNCT dti-1354 6 14 like like ADJ dti-1354 6 15 ” " PUNCT dti-1354 6 16 symptoms symptom NOUN dti-1354 6 17 that that PRON dti-1354 6 18 can can AUX dti-1354 6 19 be be AUX dti-1354 6 20 fatal fatal ADJ dti-1354 6 21 in in ADP dti-1354 6 22 immunocompromised immunocompromise VERB dti-1354 6 23 people people NOUN dti-1354 6 24 . . PUNCT dti-1354 7 1 owing owe VERB dti-1354 7 2 to to ADP dti-1354 7 3 lack lack NOUN dti-1354 7 4 of of ADP dti-1354 7 5 specific specific ADJ dti-1354 7 6 therapeutic therapeutic ADJ dti-1354 7 7 regiment regiment NOUN dti-1354 7 8 against against ADP dti-1354 7 9 the the DET dti-1354 7 10 disease disease NOUN dti-1354 7 11 , , PUNCT dti-1354 7 12 we we PRON dti-1354 7 13 cloned clone VERB dti-1354 7 14 and and CCONJ dti-1354 7 15 characterized characterize VERB dti-1354 7 16 b. b. PROPN dti-1354 7 17 microti microti PROPN dti-1354 7 18 lactate lactate PROPN dti-1354 7 19 dehydrogenase dehydrogenase NOUN dti-1354 7 20 ( ( PUNCT dti-1354 7 21 bmldh bmldh ADJ dti-1354 7 22 ) ) PUNCT dti-1354 7 23 as as ADP dti-1354 7 24 a a DET dti-1354 7 25 potential potential ADJ dti-1354 7 26 molecular molecular ADJ dti-1354 7 27 drug drug NOUN dti-1354 7 28 receptor receptor NOUN dti-1354 7 29 . . PUNCT dti-1354 8 1 the the DET dti-1354 8 2 in in ADV dti-1354 8 3 vitro vitro X dti-1354 8 4 kinetic kinetic ADJ dti-1354 8 5 properties property NOUN dti-1354 8 6 of of ADP dti-1354 8 7 bmldh bmldh ADJ dti-1354 8 8 enzyme enzyme NOUN dti-1354 8 9 was be AUX dti-1354 8 10 evaluated evaluate VERB dti-1354 8 11 using use VERB dti-1354 8 12 nicotinamide nicotinamide NOUN dti-1354 8 13 adenine adenine ADJ dti-1354 8 14 dinucleotide dinucleotide NOUN dti-1354 8 15 ( ( PUNCT dti-1354 8 16 nad+ nad+ NOUN dti-1354 8 17 ) ) PUNCT dti-1354 8 18 as as ADP dti-1354 8 19 a a DET dti-1354 8 20 co co NOUN dti-1354 8 21 - - NOUN dti-1354 8 22 factor factor NOUN dti-1354 8 23 and and CCONJ dti-1354 8 24 lactate lactate NOUN dti-1354 8 25 as as ADP dti-1354 8 26 a a DET dti-1354 8 27 substrate substrate NOUN dti-1354 8 28 . . PUNCT dti-1354 9 1 inhibitory inhibitory ADJ dti-1354 9 2 assay assay NOUN dti-1354 9 3 was be AUX dti-1354 9 4 also also ADV dti-1354 9 5 done do VERB dti-1354 9 6 using use VERB dti-1354 9 7 gossypol gossypol PROPN dti-1354 9 8 as as ADP dti-1354 9 9 bmldh bmldh ADJ dti-1354 9 10 inhibitor inhibitor NOUN dti-1354 9 11 to to PART dti-1354 9 12 determine determine VERB dti-1354 9 13 the the DET dti-1354 9 14 inhibitory inhibitory ADJ dti-1354 9 15 concentration concentration NOUN dti-1354 9 16 50 50 NUM dti-1354 9 17 ( ( PUNCT dti-1354 9 18 ic50 ic50 PROPN dti-1354 9 19 ) ) PUNCT dti-1354 9 20 . . PUNCT dti-1354 10 1 the the DET dti-1354 10 2 result result NOUN dti-1354 10 3 showed show VERB dti-1354 10 4 that that SCONJ dti-1354 10 5 the the DET dti-1354 10 6 0.99 0.99 NUM dti-1354 10 7     SPACE dti-1354 10 8 kbp kbp PROPN dti-1354 10 9 bmldh bmldh ADJ dti-1354 10 10 gene gene NOUN dti-1354 10 11 codes code NOUN dti-1354 10 12 for for ADP dti-1354 10 13 a a DET dti-1354 10 14 barely barely ADV dti-1354 10 15 soluble soluble ADJ dti-1354 10 16 36 36 NUM dti-1354 10 17     SPACE dti-1354 10 18 kda kda NOUN dti-1354 10 19 protein protein NOUN dti-1354 10 20 ( ( PUNCT dti-1354 10 21 332 332 NUM dti-1354 10 22 amino amino NOUN dti-1354 10 23 acids acid NOUN dti-1354 10 24 ) ) PUNCT dti-1354 10 25 localized localize VERB dti-1354 10 26 in in ADP dti-1354 10 27 both both CCONJ dti-1354 10 28 the the DET dti-1354 10 29 cytoplasm cytoplasm NOUN dti-1354 10 30 and and CCONJ dti-1354 10 31 nucleus nucleus NOUN dti-1354 10 32 of of ADP dti-1354 10 33 the the DET dti-1354 10 34 parasite parasite NOUN dti-1354 10 35 . . PUNCT dti-1354 11 1 in in ADP dti-1354 11 2 vitro vitro X dti-1354 11 3 enzyme enzyme NOUN dti-1354 11 4 kinetic kinetic ADJ dti-1354 11 5 studies study NOUN dti-1354 11 6 further far ADV dti-1354 11 7 revealed reveal VERB dti-1354 11 8 that that SCONJ dti-1354 11 9 bmldh bmldh PROPN dti-1354 11 10 is be AUX dti-1354 11 11 an an DET dti-1354 11 12 active active ADJ dti-1354 11 13 enzyme enzyme NOUN dti-1354 11 14 with with ADP dti-1354 11 15 a a DET dti-1354 11 16 high high ADJ dti-1354 11 17 catalytic catalytic ADJ dti-1354 11 18 efficiency efficiency NOUN dti-1354 11 19 at at ADP dti-1354 11 20 optimal optimal ADJ dti-1354 11 21 ph ph NOUN dti-1354 11 22 of of ADP dti-1354 11 23 10.2 10.2 NUM dti-1354 11 24 . . PUNCT dti-1354 12 1 the the DET dti-1354 12 2 km km NOUN dti-1354 12 3 values value NOUN dti-1354 12 4 of of ADP dti-1354 12 5 nad+ nad+ PROPN dti-1354 12 6 and and CCONJ dti-1354 12 7 lactate lactate PROPN dti-1354 12 8 were be AUX dti-1354 12 9 8.7 8.7 NUM dti-1354 12 10     SPACE dti-1354 12 11 ± ± NOUN dti-1354 12 12     SPACE dti-1354 12 13 0.57 0.57 NUM dti-1354 12 14     SPACE dti-1354 12 15 mm mm PROPN dti-1354 12 16 and and CCONJ dti-1354 12 17 99.9 99.9 NUM dti-1354 12 18     SPACE dti-1354 12 19 ± ± NOUN dti-1354 12 20     SPACE dti-1354 12 21 22.33 22.33 NUM dti-1354 12 22     SPACE dti-1354 12 23 mm mm PROPN dti-1354 12 24 , , PUNCT dti-1354 12 25 respectively respectively ADV dti-1354 12 26 . . PUNCT dti-1354 13 1 the the DET dti-1354 13 2 ic50 ic50 PROPN dti-1354 13 3 value value NOUN dti-1354 13 4 for for ADP dti-1354 13 5 gossypol gossypol PROPN dti-1354 13 6 was be AUX dti-1354 13 7 0.345 0.345 NUM dti-1354 13 8     SPACE dti-1354 13 9 µm µm NOUN dti-1354 13 10 , , PUNCT dti-1354 13 11 while while SCONJ dti-1354 13 12 at at ADP dti-1354 13 13 2.5 2.5 NUM dti-1354 13 14     SPACE dti-1354 13 15 µm µm NOUN dti-1354 13 16 , , PUNCT dti-1354 13 17 gossypol gossypol PROPN dti-1354 13 18 caused cause VERB dti-1354 13 19 100 100 NUM dti-1354 13 20 % % NOUN dti-1354 13 21 inhibition inhibition NOUN dti-1354 13 22 of of ADP dti-1354 13 23 bmldh bmldh ADJ dti-1354 13 24 catalytic catalytic ADJ dti-1354 13 25 activity activity NOUN dti-1354 13 26 . . PUNCT dti-1354 14 1 these these DET dti-1354 14 2 findings finding NOUN dti-1354 14 3 , , PUNCT dti-1354 14 4 therefore therefore ADV dti-1354 14 5 , , PUNCT dti-1354 14 6 provide provide VERB dti-1354 14 7 initial initial ADJ dti-1354 14 8 evidence evidence NOUN dti-1354 14 9 that that SCONJ dti-1354 14 10 bmldh bmldh PROPN dti-1354 14 11 could could AUX dti-1354 14 12 be be AUX dti-1354 14 13 a a DET dti-1354 14 14 potential potential ADJ dti-1354 14 15 drug drug NOUN dti-1354 14 16 target target NOUN dti-1354 14 17 , , PUNCT dti-1354 14 18 although although SCONJ dti-1354 14 19 further far ADV dti-1354 14 20 in in ADP dti-1354 14 21 vivo vivo ADJ dti-1354 14 22 studies study NOUN dti-1354 14 23 are be AUX dti-1354 14 24 needed need VERB dti-1354 14 25 to to PART dti-1354 14 26 validate validate VERB dti-1354 14 27 the the DET dti-1354 14 28 practical practical ADJ dti-1354 14 29 application application NOUN dti-1354 14 30 of of ADP dti-1354 14 31 lactate lactate NOUN dti-1354 14 32 dehydrogenase dehydrogenase NOUN dti-1354 14 33 inhibitors inhibitor NOUN dti-1354 14 34 against against ADP dti-1354 14 35 b. b. PROPN dti-1354 14 36 microti microti PROPN dti-1354 14 37 infection infection NOUN dti-1354 14 38 . . PUNCT dti-1354 15 1 key key ADJ dti-1354 15 2 words word NOUN dti-1354 15 3 : : PUNCT dti-1354 15 4 babesia babesia NOUN dti-1354 15 5 microti microti PROPN dti-1354 15 6 gray gray PROPN dti-1354 15 7 , , PUNCT dti-1354 15 8 lactate lactate NOUN dti-1354 15 9 dehydrogenenase dehydrogenenase NOUN dti-1354 15 10 , , PUNCT dti-1354 15 11 drug drug NOUN dti-1354 15 12 target target NOUN dti-1354 15 13 , , PUNCT dti-1354 15 14 gossypol gossypol PROPN dti-1354 15 15 citation citation NOUN dti-1354 15 16 : : PUNCT dti-1354 15 17 vudriko vudriko NOUN dti-1354 15 18 et et PROPN dti-1354 15 19 al al PROPN dti-1354 15 20 . . PROPN dti-1354 16 1 molecular molecular ADJ dti-1354 16 2 and and CCONJ dti-1354 16 3 kinetic kinetic ADJ dti-1354 16 4 characterization characterization NOUN dti-1354 16 5 of of ADP dti-1354 16 6 babesia babesia NOUN dti-1354 16 7 microti microti PROPN dti-1354 16 8 gray gray ADJ dti-1354 16 9 strain strain NOUN dti-1354 16 10 lactate lactate NOUN dti-1354 16 11 dehydrogenase dehydrogenase NOUN dti-1354 16 12 as as ADP dti-1354 16 13 a a DET dti-1354 16 14 potential potential ADJ dti-1354 16 15 drug drug NOUN dti-1354 16 16 target target NOUN dti-1354 16 17 . . PUNCT dti-1354 17 1 drug drug NOUN dti-1354 17 2 target target NOUN dti-1354 17 3 insights insight VERB dti-1354 17 4 2014:8 2014:8 NUM dti-1354 17 5 31–38 31–38 NUM dti-1354 17 6 doi:10.4137 doi:10.4137 NOUN dti-1354 17 7 / / SYM dti-1354 17 8 dti.s16504 dti.s16504 PUNCT dti-1354 17 9 . . PUNCT dti-1354 18 1 received receive VERB dti-1354 18 2 : : PUNCT dti-1354 18 3 april april PROPN dti-1354 18 4 27 27 NUM dti-1354 18 5 , , PUNCT dti-1354 18 6 2014 2014 NUM dti-1354 18 7 . . PUNCT dti-1354 19 1 resubmitted resubmitte VERB dti-1354 19 2 : : PUNCT dti-1354 19 3 may may AUX dti-1354 19 4 27 27 NUM dti-1354 19 5 , , PUNCT dti-1354 19 6 2014 2014 NUM dti-1354 19 7 . . PUNCT dti-1354 20 1 accepted accept VERB dti-1354 20 2 for for ADP dti-1354 20 3 publication publication NOUN dti-1354 20 4 : : PUNCT dti-1354 20 5 june june PROPN dti-1354 20 6 3 3 NUM dti-1354 20 7 , , PUNCT dti-1354 20 8 2014 2014 NUM dti-1354 20 9 . . PUNCT dti-1354 21 1 academic academic ADJ dti-1354 21 2 editor editor NOUN dti-1354 21 3 : : PUNCT dti-1354 21 4 anuj anuj PROPN dti-1354 21 5 chauhan chauhan PROPN dti-1354 21 6 , , PUNCT dti-1354 21 7 editor editor NOUN dti-1354 21 8 in in ADP dti-1354 21 9 chief chief ADJ dti-1354 21 10 type type NOUN dti-1354 21 11 : : PUNCT dti-1354 21 12 original original ADJ dti-1354 21 13 research research NOUN dti-1354 21 14 funding funding NOUN dti-1354 21 15 : : PUNCT dti-1354 21 16 this this DET dti-1354 21 17 study study NOUN dti-1354 21 18 was be AUX dti-1354 21 19 supported support VERB dti-1354 21 20 by by ADP dti-1354 21 21 japanese japanese ADJ dti-1354 21 22 international international PROPN dti-1354 21 23 cooperation cooperation NOUN dti-1354 21 24 agency agency PROPN dti-1354 21 25 ( ( PUNCT dti-1354 21 26 jica jica PROPN dti-1354 21 27 ) ) PUNCT dti-1354 21 28 . . PUNCT dti-1354 22 1 competing compete VERB dti-1354 22 2 interests interest NOUN dti-1354 22 3 : : PUNCT dti-1354 22 4 authors author NOUN dti-1354 22 5 disclose disclose VERB dti-1354 22 6 no no DET dti-1354 22 7 potential potential ADJ dti-1354 22 8 conflicts conflict NOUN dti-1354 22 9 of of ADP dti-1354 22 10 interest interest NOUN dti-1354 22 11 . . PUNCT dti-1354 23 1 copyright copyright NOUN dti-1354 23 2 : : PUNCT dti-1354 23 3 © © PROPN dti-1354 23 4 the the DET dti-1354 23 5 authors author NOUN dti-1354 23 6 , , PUNCT dti-1354 23 7 publisher publisher NOUN dti-1354 23 8 and and CCONJ dti-1354 23 9 licensee licensee NOUN dti-1354 23 10 libertas liberta NOUN dti-1354 23 11 academica academica PROPN dti-1354 23 12 limited limit VERB dti-1354 23 13 . . PUNCT dti-1354 24 1 this this PRON dti-1354 24 2 is be AUX dti-1354 24 3 an an DET dti-1354 24 4 open open ADJ dti-1354 24 5 - - PUNCT dti-1354 24 6 access access NOUN dti-1354 24 7 article article NOUN dti-1354 24 8 distributed distribute VERB dti-1354 24 9 under under ADP dti-1354 24 10 the the DET dti-1354 24 11 terms term NOUN dti-1354 24 12 of of ADP dti-1354 24 13 the the DET dti-1354 24 14 creative creative ADJ dti-1354 24 15 commons common NOUN dti-1354 24 16 cc cc NOUN dti-1354 24 17 - - PUNCT dti-1354 24 18 by by ADP dti-1354 24 19 - - PUNCT dti-1354 24 20 nc nc PROPN dti-1354 24 21 3.0 3.0 NUM dti-1354 24 22 license license NOUN dti-1354 24 23 . . PUNCT dti-1354 25 1 correspondence correspondence NOUN dti-1354 25 2 : : PUNCT dti-1354 25 3 vpato@covab.mak.ac.ug vpato@covab.mak.ac.ug NOUN dti-1354 25 4 ; ; PUNCT dti-1354 25 5 gen@obihiro.ac.jp gen@obihiro.ac.jp NOUN dti-1354 25 6 this this DET dti-1354 25 7 paper paper NOUN dti-1354 25 8 was be AUX dti-1354 25 9 subject subject ADJ dti-1354 25 10 to to ADP dti-1354 25 11 independent independent ADJ dti-1354 25 12 , , PUNCT dti-1354 25 13 expert expert ADJ dti-1354 25 14 peer peer NOUN dti-1354 25 15 review review NOUN dti-1354 25 16 by by ADP dti-1354 25 17 a a DET dti-1354 25 18 minimum minimum NOUN dti-1354 25 19 of of ADP dti-1354 25 20 two two NUM dti-1354 25 21 blind blind ADJ dti-1354 25 22 peer peer NOUN dti-1354 25 23 reviewers reviewer NOUN dti-1354 25 24 . . PUNCT dti-1354 26 1 all all DET dti-1354 26 2 editorial editorial ADJ dti-1354 26 3 decisions decision NOUN dti-1354 26 4 were be AUX dti-1354 26 5 made make VERB dti-1354 26 6 by by ADP dti-1354 26 7 the the DET dti-1354 26 8 independent independent ADJ dti-1354 26 9 academic academic ADJ dti-1354 26 10 editor editor NOUN dti-1354 26 11 . . PUNCT dti-1354 27 1 all all DET dti-1354 27 2 authors author NOUN dti-1354 27 3 have have AUX dti-1354 27 4 provided provide VERB dti-1354 27 5 signed sign VERB dti-1354 27 6 confirmation confirmation NOUN dti-1354 27 7 of of ADP dti-1354 27 8 their their PRON dti-1354 27 9 compliance compliance NOUN dti-1354 27 10 with with ADP dti-1354 27 11 ethical ethical ADJ dti-1354 27 12 and and CCONJ dti-1354 27 13 legal legal ADJ dti-1354 27 14 obligations obligation NOUN dti-1354 27 15 including include VERB dti-1354 27 16 ( ( PUNCT dti-1354 27 17 but but CCONJ dti-1354 27 18 not not PART dti-1354 27 19 limited limit VERB dti-1354 27 20 to to ADP dti-1354 27 21 ) ) PUNCT dti-1354 27 22 use use NOUN dti-1354 27 23 of of ADP dti-1354 27 24 any any DET dti-1354 27 25 copyrighted copyright VERB dti-1354 27 26 material material NOUN dti-1354 27 27 , , PUNCT dti-1354 27 28 compliance compliance NOUN dti-1354 27 29 with with ADP dti-1354 27 30 icmje icmje NOUN dti-1354 27 31 authorship authorship NOUN dti-1354 27 32 and and CCONJ dti-1354 27 33 competing compete VERB dti-1354 27 34 interests interest NOUN dti-1354 27 35 disclosure disclosure NOUN dti-1354 27 36 guidelines guideline NOUN dti-1354 27 37 and and CCONJ dti-1354 27 38 , , PUNCT dti-1354 27 39 where where SCONJ dti-1354 27 40 applicable applicable ADJ dti-1354 27 41 , , PUNCT dti-1354 27 42 compliance compliance NOUN dti-1354 27 43 with with ADP dti-1354 27 44 legal legal ADJ dti-1354 27 45 and and CCONJ dti-1354 27 46 ethical ethical ADJ dti-1354 27 47 guidelines guideline NOUN dti-1354 27 48 on on ADP dti-1354 27 49 human human ADJ dti-1354 27 50 and and CCONJ dti-1354 27 51 animal animal NOUN dti-1354 27 52 research research NOUN dti-1354 27 53 participants participant NOUN dti-1354 27 54 . . PUNCT dti-1354 28 1 introduction introduction NOUN dti-1354 28 2 babesia babesia NOUN dti-1354 28 3 microti microti PROPN dti-1354 28 4 is be AUX dti-1354 28 5 a a DET dti-1354 28 6 protozoan protozoan NOUN dti-1354 28 7 organism organism NOUN dti-1354 28 8 ( ( PUNCT dti-1354 28 9 piroplasmida piroplasmida PROPN dti-1354 28 10 : : PUNCT dti-1354 28 11 apicomplexa apicomplexa PROPN dti-1354 28 12 ) ) PUNCT dti-1354 28 13 that that PRON dti-1354 28 14 infects infect VERB dti-1354 28 15 mainly mainly ADV dti-1354 28 16 rodents rodent NOUN dti-1354 28 17 and and CCONJ dti-1354 28 18 humans.1 humans.1 VERB dti-1354 28 19 through through ADP dti-1354 28 20 phylogenetic phylogenetic ADJ dti-1354 28 21 investigations,2 investigations,2 NOUN dti-1354 28 22 b. b. PROPN dti-1354 28 23 microti microti PROPN dti-1354 28 24 has have AUX dti-1354 28 25 been be AUX dti-1354 28 26 classified classify VERB dti-1354 28 27 into into ADP dti-1354 28 28 four four NUM dti-1354 28 29 subtypes subtype NOUN dti-1354 28 30 , , PUNCT dti-1354 28 31 namely namely ADV dti-1354 28 32 us we PRON dti-1354 28 33 ( ( PUNCT dti-1354 28 34 r1 r1 NOUN dti-1354 28 35 and and CCONJ dti-1354 28 36 gray gray ADJ dti-1354 28 37 strains strain NOUN dti-1354 28 38 ) ) PUNCT dti-1354 28 39 , , PUNCT dti-1354 28 40 munich munich PROPN dti-1354 28 41 , , PUNCT dti-1354 28 42 kobe kobe PROPN dti-1354 28 43 , , PUNCT dti-1354 28 44 and and CCONJ dti-1354 28 45 hobetsu hobetsu NOUN dti-1354 28 46 . . PUNCT dti-1354 29 1 recent recent ADJ dti-1354 29 2 studies study NOUN dti-1354 29 3 suggest suggest VERB dti-1354 29 4 that that SCONJ dti-1354 29 5 b. b. PROPN dti-1354 29 6 microti microti PROPN dti-1354 29 7 has have VERB dti-1354 29 8 a a DET dti-1354 29 9 wide wide ADJ dti-1354 29 10 distribution distribution NOUN dti-1354 29 11 of of ADP dti-1354 29 12 wild wild ADJ dti-1354 29 13 reservoirs reservoir NOUN dti-1354 29 14 in in ADP dti-1354 29 15 the the DET dti-1354 29 16 united united PROPN dti-1354 29 17 states states PROPN dti-1354 29 18 of of ADP dti-1354 29 19 america3 america3 NOUN dti-1354 29 20 and and CCONJ dti-1354 29 21 northeastern northeastern NOUN dti-1354 29 22 eurasia.4 eurasia.4 ADP dti-1354 29 23 the the DET dti-1354 29 24 emerging emerge VERB dti-1354 29 25 zoonotic zoonotic ADJ dti-1354 29 26 importance importance NOUN dti-1354 29 27 of of ADP dti-1354 29 28 b. b. PROPN dti-1354 29 29 microti microti PROPN dti-1354 29 30 has have AUX dti-1354 29 31 been be AUX dti-1354 29 32 widely widely ADV dti-1354 29 33 reported.5–7 reported.5–7 VERB dti-1354 29 34 the the DET dti-1354 29 35 organism organism NOUN dti-1354 29 36 has have AUX dti-1354 29 37 been be AUX dti-1354 29 38 implicated implicate VERB dti-1354 29 39 as as ADP dti-1354 29 40 a a DET dti-1354 29 41 major major ADJ dti-1354 29 42 concern concern NOUN dti-1354 29 43 for for ADP dti-1354 29 44 the the DET dti-1354 29 45 safety safety NOUN dti-1354 29 46 of of ADP dti-1354 29 47 blood blood NOUN dti-1354 29 48 transfusion transfusion NOUN dti-1354 29 49 supply supply NOUN dti-1354 29 50 in in ADP dti-1354 29 51 usa.8,9 usa.8,9 PROPN dti-1354 29 52 human human ADJ dti-1354 29 53 co co NOUN dti-1354 29 54 - - NOUN dti-1354 29 55 infection infection NOUN dti-1354 29 56 of of ADP dti-1354 29 57 patients patient NOUN dti-1354 29 58 with with ADP dti-1354 29 59 both both DET dti-1354 29 60 b. b. PROPN dti-1354 29 61 microti microti PROPN dti-1354 29 62 and and CCONJ dti-1354 29 63 other other ADJ dti-1354 29 64 zoonotic zoonotic ADJ dti-1354 29 65 tick tick NOUN dti-1354 29 66 - - PUNCT dti-1354 29 67 borne bear VERB dti-1354 29 68 hemoparasites hemoparasite NOUN dti-1354 29 69 has have AUX dti-1354 29 70 been be AUX dti-1354 29 71 reported.10 reported.10 NOUN dti-1354 29 72 moreover moreover ADV dti-1354 29 73 , , PUNCT dti-1354 29 74 the the DET dti-1354 29 75 parasite parasite NOUN dti-1354 29 76 causes cause VERB dti-1354 29 77 “ " PUNCT dti-1354 29 78 malaria malaria NOUN dti-1354 29 79 - - PUNCT dti-1354 29 80 like like ADJ dti-1354 29 81 ” " PUNCT dti-1354 29 82 symptoms symptom NOUN dti-1354 29 83 in in ADP dti-1354 29 84 immunocompetent immunocompetent ADJ dti-1354 29 85 individuals individual NOUN dti-1354 29 86 and and CCONJ dti-1354 29 87 can can AUX dti-1354 29 88 potentially potentially ADV dti-1354 29 89 lead lead VERB dti-1354 29 90 to to ADP dti-1354 29 91 fatal fatal ADJ dti-1354 29 92 relapsing relapse VERB dti-1354 29 93 illness illness NOUN dti-1354 29 94 in in ADP dti-1354 29 95 immunocompromised immunocompromise VERB dti-1354 29 96 patients.11 patients.11 NOUN dti-1354 29 97 the the DET dti-1354 29 98 importance importance NOUN dti-1354 29 99 of of ADP dti-1354 29 100 b. b. PROPN dti-1354 29 101 microti microti PROPN dti-1354 29 102 as as ADP dti-1354 29 103 an an DET dti-1354 29 104 emerging emerge VERB dti-1354 29 105 zoonotic zoonotic ADJ dti-1354 29 106 disease disease NOUN dti-1354 29 107 has have AUX dti-1354 29 108 created create VERB dti-1354 29 109 an an DET dti-1354 29 110 urgent urgent ADJ dti-1354 29 111 need need NOUN dti-1354 29 112 for for ADP dti-1354 29 113 innovation innovation NOUN dti-1354 29 114 of of ADP dti-1354 29 115 effective effective ADJ dti-1354 29 116 chemotherapeutic chemotherapeutic ADJ dti-1354 29 117 agents agent NOUN dti-1354 29 118 for for ADP dti-1354 29 119 its its PRON dti-1354 29 120 treatment treatment NOUN dti-1354 29 121 . . PUNCT dti-1354 30 1 a a DET dti-1354 30 2 combination combination NOUN dti-1354 30 3 of of ADP dti-1354 30 4 atovaquone atovaquone NOUN dti-1354 30 5 and and CCONJ dti-1354 30 6 azithromycin azithromycin NOUN dti-1354 30 7 as as ADV dti-1354 30 8 well well ADV dti-1354 30 9 as as ADP dti-1354 30 10 clindamycin clindamycin NOUN dti-1354 30 11 and and CCONJ dti-1354 30 12 quinine quinine NOUN dti-1354 30 13 were be AUX dti-1354 30 14 reported report VERB dti-1354 30 15 as as ADP dti-1354 30 16 effective effective ADJ dti-1354 30 17 against against ADP dti-1354 30 18 b. b. PROPN dti-1354 30 19 microti.12 microti.12 PROPN dti-1354 30 20 however however ADV dti-1354 30 21 , , PUNCT dti-1354 30 22 recrudescence recrudescence NOUN dti-1354 30 23 and and CCONJ dti-1354 30 24 resistance resistance NOUN dti-1354 30 25 against against ADP dti-1354 30 26 atovaquone atovaquone NOUN dti-1354 30 27 and and CCONJ dti-1354 30 28 lack lack NOUN dti-1354 30 29 of of ADP dti-1354 30 30 http://www.la-press.com http://www.la-press.com X dti-1354 30 31 http://www.la-press.com http://www.la-press.com X dti-1354 31 1 http://www.la-press.com/drug-target-insights-journal-j23 http://www.la-press.com/drug-target-insights-journal-j23 AUX dti-1354 31 2 http://dx.doi.org/10.4137/dti.s16504 http://dx.doi.org/10.4137/dti.s16504 ADV dti-1354 31 3 http://creativecommons.org/licenses/by-nc/3.0/ http://creativecommons.org/licenses/by-nc/3.0/ NOUN dti-1354 31 4 http://creativecommons.org/licenses/by-nc/3.0/ http://creativecommons.org/licenses/by-nc/3.0/ NOUN dti-1354 31 5 mailto:vpato@covab.mak.ac.ug mailto:vpato@covab.mak.ac.ug NOUN dti-1354 31 6 mailto:gen@obihiro.ac.jp mailto:gen@obihiro.ac.jp NOUN dti-1354 31 7 vudriko vudriko PROPN dti-1354 31 8 et et PROPN dti-1354 31 9 al al PROPN dti-1354 31 10 32 32 NUM dti-1354 31 11 drug drug NOUN dti-1354 31 12 target target NOUN dti-1354 31 13 insights insight VERB dti-1354 31 14 2014:8 2014:8 NUM dti-1354 31 15 enzymes enzyme NOUN dti-1354 31 16 involved involve VERB dti-1354 31 17 in in ADP dti-1354 31 18 heme heme NOUN dti-1354 31 19 metabolism metabolism NOUN dti-1354 31 20 limits limit VERB dti-1354 31 21 the the DET dti-1354 31 22 use use NOUN dti-1354 31 23 of of ADP dti-1354 31 24 atovaquone atovaquone NOUN dti-1354 31 25 and and CCONJ dti-1354 31 26 quinoline quinoline NOUN dti-1354 31 27 - - PUNCT dti-1354 31 28 derived derive VERB dti-1354 31 29 drugs drug NOUN dti-1354 31 30 such such ADJ dti-1354 31 31 as as ADP dti-1354 31 32 quinine quinine NOUN dti-1354 31 33 and and CCONJ dti-1354 31 34 chloroquine chloroquine NOUN dti-1354 31 35 in in ADP dti-1354 31 36 the the DET dti-1354 31 37 treatment treatment NOUN dti-1354 31 38 of of ADP dti-1354 31 39 b. b. PROPN dti-1354 31 40 microti microti PROPN dti-1354 31 41 infections.13,14 infections.13,14 PROPN dti-1354 31 42 lactate lactate PROPN dti-1354 31 43 dehydrogenase dehydrogenase NOUN dti-1354 31 44 ( ( PUNCT dti-1354 31 45 ldh ldh PROPN dti-1354 31 46 ) ) PUNCT dti-1354 31 47 is be AUX dti-1354 31 48 one one NUM dti-1354 31 49 of of ADP dti-1354 31 50 the the DET dti-1354 31 51 apicomplexan apicomplexan NOUN dti-1354 31 52 glycolytic glycolytic ADJ dti-1354 31 53 enzymes enzyme NOUN dti-1354 31 54 that that PRON dti-1354 31 55 catalyze catalyze NOUN dti-1354 31 56 interconversion interconversion NOUN dti-1354 31 57 of of ADP dti-1354 31 58 pyruvate pyruvate NOUN dti-1354 31 59 to to PART dti-1354 31 60 lactate.15 lactate.15 VERB dti-1354 31 61 the the DET dti-1354 31 62 enzyme enzyme NOUN dti-1354 31 63 plays play VERB dti-1354 31 64 indispensable indispensable ADJ dti-1354 31 65 role role NOUN dti-1354 31 66 in in ADP dti-1354 31 67 apicomplexan apicomplexan NOUN dti-1354 31 68 energy energy NOUN dti-1354 31 69 metabolism metabolism NOUN dti-1354 31 70 using use VERB dti-1354 31 71 nad+ nad+ PROPN dti-1354 31 72 as as ADP dti-1354 31 73 a a DET dti-1354 31 74 cofactor cofactor NOUN dti-1354 31 75 under under ADP dti-1354 31 76 anaerobic anaerobic NOUN dti-1354 31 77 conditions.16 conditions.16 PROPN dti-1354 31 78 the the DET dti-1354 31 79 resultant resultant NOUN dti-1354 31 80 energy energy NOUN dti-1354 31 81 generated generate VERB dti-1354 31 82 is be AUX dti-1354 31 83 used use VERB dti-1354 31 84 by by ADP dti-1354 31 85 parasites parasite NOUN dti-1354 31 86 for for ADP dti-1354 31 87 their their PRON dti-1354 31 88 biochemical biochemical ADJ dti-1354 31 89 processes process NOUN dti-1354 31 90 and and CCONJ dti-1354 31 91 survival survival NOUN dti-1354 31 92 . . PUNCT dti-1354 32 1 available available ADJ dti-1354 32 2 reports report NOUN dti-1354 32 3 indicate indicate VERB dti-1354 32 4 that that SCONJ dti-1354 32 5 the the DET dti-1354 32 6 enzyme enzyme NOUN dti-1354 32 7 is be AUX dti-1354 32 8 a a DET dti-1354 32 9 novel novel ADJ dti-1354 32 10 drug drug NOUN dti-1354 32 11 target target NOUN dti-1354 32 12 in in ADP dti-1354 32 13 plasmodium plasmodium NOUN dti-1354 32 14 spp spp NOUN dti-1354 32 15 , , PUNCT dti-1354 32 16 toxoplasma toxoplasma NOUN dti-1354 32 17 gondii gondii NOUN dti-1354 32 18 , , PUNCT dti-1354 32 19 theileria theileria NOUN dti-1354 32 20 annulata annulata PROPN dti-1354 32 21 , , PUNCT dti-1354 32 22 and and CCONJ dti-1354 32 23 babesia babesia NOUN dti-1354 32 24 bovis.15,17–19 bovis.15,17–19 NOUN dti-1354 32 25 for for ADP dti-1354 32 26 this this DET dti-1354 32 27 reason reason NOUN dti-1354 32 28 , , PUNCT dti-1354 32 29 there there PRON dti-1354 32 30 is be VERB dti-1354 32 31 a a DET dti-1354 32 32 growing grow VERB dti-1354 32 33 momentum momentum NOUN dti-1354 32 34 in in ADP dti-1354 32 35 the the DET dti-1354 32 36 synthesis synthesis NOUN dti-1354 32 37 of of ADP dti-1354 32 38 new new ADJ dti-1354 32 39 ldh ldh NOUN dti-1354 32 40 inhibitors inhibitor NOUN dti-1354 32 41 as as ADP dti-1354 32 42 potential potential ADJ dti-1354 32 43 drugs drug NOUN dti-1354 32 44 against against ADP dti-1354 32 45 the the DET dti-1354 32 46 above above ADJ dti-1354 32 47 organisms.20 organisms.20 PROPN dti-1354 32 48 however however ADV dti-1354 32 49 , , PUNCT dti-1354 32 50 to to ADP dti-1354 32 51 the the DET dti-1354 32 52 best good ADJ dti-1354 32 53 of of ADP dti-1354 32 54 our our PRON dti-1354 32 55 knowledge knowledge NOUN dti-1354 32 56 , , PUNCT dti-1354 32 57 there there PRON dti-1354 32 58 is be VERB dti-1354 32 59 still still ADV dti-1354 32 60 paucity paucity NOUN dti-1354 32 61 of of ADP dti-1354 32 62 scientific scientific ADJ dti-1354 32 63 evidence evidence NOUN dti-1354 32 64 on on ADP dti-1354 32 65 the the DET dti-1354 32 66 suitability suitability NOUN dti-1354 32 67 of of ADP dti-1354 32 68 b. b. PROPN dti-1354 32 69 microti microti PROPN dti-1354 32 70 ldh ldh PROPN dti-1354 32 71 as as ADP dti-1354 32 72 a a DET dti-1354 32 73 potential potential ADJ dti-1354 32 74 drug drug NOUN dti-1354 32 75 target target NOUN dti-1354 32 76 . . PUNCT dti-1354 33 1 therefore therefore ADV dti-1354 33 2 , , PUNCT dti-1354 33 3 the the DET dti-1354 33 4 aim aim NOUN dti-1354 33 5 of of ADP dti-1354 33 6 the the DET dti-1354 33 7 current current ADJ dti-1354 33 8 study study NOUN dti-1354 33 9 was be AUX dti-1354 33 10 to to ADP dti-1354 33 11 clone clone PROPN dti-1354 33 12 b. b. PROPN dti-1354 33 13 microti microti PROPN dti-1354 33 14 lactate lactate PROPN dti-1354 33 15 dehydrogenase dehydrogenase NOUN dti-1354 33 16 ( ( PUNCT dti-1354 33 17 bmldh bmldh ADJ dti-1354 33 18 ) ) PUNCT dti-1354 33 19 , , PUNCT dti-1354 33 20 characterize characterize VERB dti-1354 33 21 the the DET dti-1354 33 22 protein protein NOUN dti-1354 33 23 , , PUNCT dti-1354 33 24 and and CCONJ dti-1354 33 25 evaluate evaluate VERB dti-1354 33 26 its its PRON dti-1354 33 27 in in ADV dti-1354 33 28 vitro vitro X dti-1354 33 29 kinetic kinetic ADJ dti-1354 33 30 properties property NOUN dti-1354 33 31 with with ADP dti-1354 33 32 the the DET dti-1354 33 33 view view NOUN dti-1354 33 34 of of ADP dti-1354 33 35 assessing assess VERB dti-1354 33 36 its its PRON dti-1354 33 37 potential potential NOUN dti-1354 33 38 as as ADP dti-1354 33 39 a a DET dti-1354 33 40 novel novel ADJ dti-1354 33 41 drug drug NOUN dti-1354 33 42 target target NOUN dti-1354 33 43 against against ADP dti-1354 33 44 b. b. PROPN dti-1354 33 45 microti microti PROPN dti-1354 33 46 infection infection NOUN dti-1354 33 47 . . PUNCT dti-1354 34 1 materials material NOUN dti-1354 34 2 and and CCONJ dti-1354 34 3 method method NOUN dti-1354 34 4 ethics ethic NOUN dti-1354 34 5 . . PUNCT dti-1354 35 1 ethical ethical ADJ dti-1354 35 2 clearance clearance NOUN dti-1354 35 3 ( ( PUNCT dti-1354 35 4 approval approval NOUN dti-1354 35 5 no no NOUN dti-1354 35 6 . . PROPN dti-1354 35 7 250035 250035 NUM dti-1354 35 8 ) ) PUNCT dti-1354 35 9 was be AUX dti-1354 35 10 sought seek VERB dti-1354 35 11 and and CCONJ dti-1354 35 12 obtained obtain VERB dti-1354 35 13 in in ADP dti-1354 35 14 accordance accordance NOUN dti-1354 35 15 with with ADP dti-1354 35 16 the the DET dti-1354 35 17 provision provision NOUN dti-1354 35 18 of of ADP dti-1354 35 19 article article NOUN dti-1354 35 20 32 32 NUM dti-1354 35 21 - - SYM dti-1354 35 22 1 1 NUM dti-1354 35 23 of of ADP dti-1354 35 24 the the DET dti-1354 35 25 regulation regulation NOUN dti-1354 35 26 of of ADP dti-1354 35 27 animal animal NOUN dti-1354 35 28 experiment experiment NOUN dti-1354 35 29 of of ADP dti-1354 35 30 obihiro obihiro PROPN dti-1354 35 31 university university PROPN dti-1354 35 32 of of ADP dti-1354 35 33 agriculture agriculture NOUN dti-1354 35 34 and and CCONJ dti-1354 35 35 veterinary veterinary ADJ dti-1354 35 36 medicine medicine NOUN dti-1354 35 37 , , PUNCT dti-1354 35 38 japan japan PROPN dti-1354 35 39 . . PUNCT dti-1354 36 1 polymerase polymerase NOUN dti-1354 36 2 chain chain NOUN dti-1354 36 3 reaction reaction NOUN dti-1354 36 4 ( ( PUNCT dti-1354 36 5 pcr pcr PROPN dti-1354 36 6 ) ) PUNCT dti-1354 36 7 , , PUNCT dti-1354 36 8 cloning cloning NOUN dti-1354 36 9 and and CCONJ dti-1354 36 10 sequencing sequencing NOUN dti-1354 36 11 . . PUNCT dti-1354 37 1 the the DET dti-1354 37 2 purified purified ADJ dti-1354 37 3 genomic genomic ADJ dti-1354 37 4 dna dna NOUN dti-1354 37 5 of of ADP dti-1354 37 6 human human PROPN dti-1354 37 7 isolate isolate PROPN dti-1354 37 8 of of ADP dti-1354 37 9 b. b. PROPN dti-1354 37 10 microti microti PROPN dti-1354 37 11 gray gray ADJ dti-1354 37 12 strain strain NOUN dti-1354 37 13 ( ( PUNCT dti-1354 37 14 us us PROPN dti-1354 37 15 type type NOUN dti-1354 37 16 , , PUNCT dti-1354 37 17 culture culture NOUN dti-1354 37 18 collection collection NOUN dti-1354 37 19 catalog catalog NOUN dti-1354 37 20 no no NOUN dti-1354 37 21 . . NOUN dti-1354 37 22 30221 30221 NUM dti-1354 37 23 ) ) PUNCT dti-1354 37 24 was be AUX dti-1354 37 25 used use VERB dti-1354 37 26 as as ADP dti-1354 37 27 template.21 template.21 PROPN dti-1354 37 28 oligo oligo ADJ dti-1354 37 29 nucleotide nucleotide ADJ dti-1354 37 30 primers primer NOUN dti-1354 37 31 with with ADP dti-1354 37 32 bamhi bamhi NOUN dti-1354 37 33 restriction restriction NOUN dti-1354 37 34 site site NOUN dti-1354 37 35 at at ADP dti-1354 37 36 the the DET dti-1354 37 37 forward forward ADJ dti-1354 37 38 ( ( PUNCT dti-1354 37 39 5′-cctcggatcccattcgttaaaagaaga 5′-cctcggatcccattcgttaaaagaaga NUM dti-1354 37 40 atttc-3′ atttc-3′ ADJ dti-1354 37 41 ) ) PUNCT dti-1354 37 42 and and CCONJ dti-1354 37 43 xhoi xhoi ADJ dti-1354 37 44 site site NOUN dti-1354 37 45 at at ADP dti-1354 37 46 the the DET dti-1354 37 47 reverse reverse NOUN dti-1354 37 48 ( ( PUNCT dti-1354 37 49 5′-ggggctcgagttatagttggatatctttctgtg-3′ 5′-ggggctcgagttatagttggatatctttctgtg-3′ NOUN dti-1354 37 50 ) ) PUNCT dti-1354 37 51 were be AUX dti-1354 37 52 designed design VERB dti-1354 37 53 from from ADP dti-1354 37 54 b. b. PROPN dti-1354 37 55 microti microti PROPN dti-1354 37 56 strain strain PROPN dti-1354 37 57 r1 r1 PROPN dti-1354 37 58 ldh ldh PROPN dti-1354 37 59 gene gene NOUN dti-1354 37 60 sequence sequence NOUN dti-1354 37 61 obtained obtain VERB dti-1354 37 62 from from ADP dti-1354 37 63 genbank genbank PROPN dti-1354 37 64 ( ( PUNCT dti-1354 37 65 accession accession NOUN dti-1354 37 66 no no INTJ dti-1354 37 67 . . PUNCT dti-1354 38 1 ccf72479).14 ccf72479).14 PROPN dti-1354 38 2 thermocycling thermocycling PROPN dti-1354 38 3 was be AUX dti-1354 38 4 done do VERB dti-1354 38 5 in in ADP dti-1354 38 6 a a DET dti-1354 38 7 50 50 NUM dti-1354 38 8     SPACE dti-1354 38 9 µl µl ADP dti-1354 38 10 reaction reaction NOUN dti-1354 38 11 volume volume NOUN dti-1354 38 12 containing contain VERB dti-1354 38 13 10 10 NUM dti-1354 38 14     SPACE dti-1354 38 15 ng ng PROPN dti-1354 38 16 genomic genomic PROPN dti-1354 38 17 dna dna NOUN dti-1354 38 18 , , PUNCT dti-1354 38 19 20 20 NUM dti-1354 38 20     SPACE dti-1354 38 21 pg pg PROPN dti-1354 38 22 primers primer NOUN dti-1354 38 23 , , PUNCT dti-1354 38 24 0.2 0.2 NUM dti-1354 38 25     SPACE dti-1354 38 26 µm µm NOUN dti-1354 38 27 dntp dntp NOUN dti-1354 38 28 ( ( PUNCT dti-1354 38 29 takara takara PROPN dti-1354 38 30 , , PUNCT dti-1354 38 31 japan japan PROPN dti-1354 38 32 ) ) PUNCT dti-1354 38 33 , , PUNCT dti-1354 38 34 and and CCONJ dti-1354 38 35 2.5 2.5 NUM dti-1354 38 36     SPACE dti-1354 38 37 u u PRON dti-1354 38 38 extaq extaq X dti-1354 38 39 polymerase polymerase NOUN dti-1354 38 40 ( ( PUNCT dti-1354 38 41 takara takara PROPN dti-1354 38 42 , , PUNCT dti-1354 38 43 japan japan PROPN dti-1354 38 44 ) ) PUNCT dti-1354 38 45 . . PUNCT dti-1354 39 1 the the DET dti-1354 39 2 pcr pcr PROPN dti-1354 39 3 condition condition NOUN dti-1354 39 4 included include VERB dti-1354 39 5 two two NUM dti-1354 39 6 minutes minute NOUN dti-1354 39 7 denaturation denaturation NOUN dti-1354 39 8 followed follow VERB dti-1354 39 9 by by ADP dti-1354 39 10 30 30 NUM dti-1354 39 11 cycles cycle NOUN dti-1354 39 12 of of ADP dti-1354 39 13 98 98 NUM dti-1354 39 14 ° ° ADP dti-1354 39 15 c c NOUN dti-1354 39 16 for for ADP dti-1354 39 17 10 10 NUM dti-1354 39 18 seconds second NOUN dti-1354 39 19 , , PUNCT dti-1354 39 20 55 55 NUM dti-1354 39 21 ° ° ADP dti-1354 39 22 c c NOUN dti-1354 39 23 for for ADP dti-1354 39 24 30 30 NUM dti-1354 39 25 seconds second NOUN dti-1354 39 26 , , PUNCT dti-1354 39 27 68 68 NUM dti-1354 39 28 ° ° ADP dti-1354 39 29 c c NOUN dti-1354 39 30 for for ADP dti-1354 39 31 50 50 NUM dti-1354 39 32 seconds second NOUN dti-1354 39 33 , , PUNCT dti-1354 39 34 and and CCONJ dti-1354 39 35 further further ADJ dti-1354 39 36 extension extension NOUN dti-1354 39 37 at at ADP dti-1354 39 38 72 72 NUM dti-1354 39 39 ° ° ADP dti-1354 39 40 c c NOUN dti-1354 39 41 for for ADP dti-1354 39 42 five five NUM dti-1354 39 43 minutes minute NOUN dti-1354 39 44 . . PUNCT dti-1354 40 1 the the DET dti-1354 40 2 resultant resultant NOUN dti-1354 40 3 polymerase polymerase NOUN dti-1354 40 4 chain chain NOUN dti-1354 40 5 reaction reaction NOUN dti-1354 40 6 ( ( PUNCT dti-1354 40 7 pcr pcr NOUN dti-1354 40 8 ) ) PUNCT dti-1354 40 9 product product NOUN dti-1354 40 10 was be AUX dti-1354 40 11 electrophoresed electrophorese VERB dti-1354 40 12 , , PUNCT dti-1354 40 13 stained stain VERB dti-1354 40 14 with with ADP dti-1354 40 15 ethidium ethidium NOUN dti-1354 40 16 , , PUNCT dti-1354 40 17 and and CCONJ dti-1354 40 18 viewed view VERB dti-1354 40 19 under under ADP dti-1354 40 20 uv uv NOUN dti-1354 40 21 transilluminator transilluminator NOUN dti-1354 40 22 . . PUNCT dti-1354 41 1 the the DET dti-1354 41 2 bmldh bmldh ADJ dti-1354 41 3 amplicon amplicon NOUN dti-1354 41 4 was be AUX dti-1354 41 5 extracted extract VERB dti-1354 41 6 and and CCONJ dti-1354 41 7 purified purify VERB dti-1354 41 8 from from ADP dti-1354 41 9 the the DET dti-1354 41 10 gel gel NOUN dti-1354 41 11 using use VERB dti-1354 41 12 qiaquick qiaquick NOUN dti-1354 41 13 gel gel NOUN dti-1354 41 14 extraction extraction NOUN dti-1354 41 15 kit kit NOUN dti-1354 41 16 ( ( PUNCT dti-1354 41 17 qiagen qiagen PROPN dti-1354 41 18 , , PUNCT dti-1354 41 19 germany germany PROPN dti-1354 41 20 ) ) PUNCT dti-1354 41 21 according accord VERB dti-1354 41 22 to to ADP dti-1354 41 23 the the DET dti-1354 41 24 manufacturer manufacturer NOUN dti-1354 41 25 ’s ’s PART dti-1354 41 26 instruction instruction NOUN dti-1354 41 27 . . PUNCT dti-1354 42 1 the the DET dti-1354 42 2 purified purified ADJ dti-1354 42 3 bmldh bmldh PROPN dti-1354 42 4 was be AUX dti-1354 42 5 cloned clone VERB dti-1354 42 6 into into ADP dti-1354 42 7 pgem pgem NOUN dti-1354 42 8 - - PUNCT dti-1354 42 9 t t NOUN dti-1354 42 10 easy easy ADJ dti-1354 42 11 vector vector NOUN dti-1354 42 12 ( ( PUNCT dti-1354 42 13 promega promega PROPN dti-1354 42 14 , , PUNCT dti-1354 42 15 usa usa PROPN dti-1354 42 16 ) ) PUNCT dti-1354 42 17 and and CCONJ dti-1354 42 18 transformed transform VERB dti-1354 42 19 into into ADP dti-1354 42 20 competent competent ADJ dti-1354 42 21 escherichia escherichia NOUN dti-1354 42 22 coli coli NOUN dti-1354 42 23 dh5α dh5α PROPN dti-1354 42 24 ( ( PUNCT dti-1354 42 25 invitrogen invitrogen PROPN dti-1354 42 26 ) ) PUNCT dti-1354 42 27 . . PUNCT dti-1354 43 1 the the DET dti-1354 43 2 resultant resultant NOUN dti-1354 43 3 pgem pgem PROPN dti-1354 43 4 - - PUNCT dti-1354 43 5 t t NOUN dti-1354 43 6 easy easy ADV dti-1354 43 7 - - PUNCT dti-1354 43 8 bmldh bmldh PROPN dti-1354 43 9 was be AUX dti-1354 43 10 purified purify VERB dti-1354 43 11 using use VERB dti-1354 43 12 nucleospin nucleospin NOUN dti-1354 43 13 ® ® NOUN dti-1354 43 14 plasmid plasmid NOUN dti-1354 43 15 kit kit NOUN dti-1354 43 16 according accord VERB dti-1354 43 17 to to ADP dti-1354 43 18 the the DET dti-1354 43 19 manufacturer manufacturer NOUN dti-1354 43 20 ’s ’s PART dti-1354 43 21 ( ( PUNCT dti-1354 43 22 macherey macherey PROPN dti-1354 43 23 - - PUNCT dti-1354 43 24 nagel nagel PROPN dti-1354 43 25 , , PUNCT dti-1354 43 26 germany germany PROPN dti-1354 43 27 ) ) PUNCT dti-1354 43 28 instruction instruction NOUN dti-1354 43 29 . . PUNCT dti-1354 44 1 sequencing sequence VERB dti-1354 44 2 was be AUX dti-1354 44 3 carried carry VERB dti-1354 44 4 out out ADP dti-1354 44 5 using use VERB dti-1354 44 6 m13 m13 NOUN dti-1354 44 7 forward forward ADV dti-1354 44 8 5′-tgtaaaacgacggccagt-3′ 5′-tgtaaaacgacggccagt-3′ NUM dti-1354 44 9 ) ) PUNCT dti-1354 44 10 and and CCONJ dti-1354 44 11 reverse reverse ADJ dti-1354 44 12 ( ( PUNCT dti-1354 44 13 5′-caggaaacagctatgacc-3′ 5′-caggaaacagctatgacc-3′ NUM dti-1354 44 14 ) ) PUNCT dti-1354 44 15 primers primer NOUN dti-1354 44 16 with with ADP dti-1354 44 17 automated automate VERB dti-1354 44 18 sequencer sequencer NOUN dti-1354 44 19 ( ( PUNCT dti-1354 44 20 abi abi PROPN dti-1354 44 21 prism prism NOUN dti-1354 44 22 3100 3100 NUM dti-1354 44 23 genetic genetic ADJ dti-1354 44 24 analyzer analyzer NOUN dti-1354 44 25 , , PUNCT dti-1354 44 26 usa usa PROPN dti-1354 44 27 ) ) PUNCT dti-1354 44 28 . . PUNCT dti-1354 45 1 expression expression NOUN dti-1354 45 2 and and CCONJ dti-1354 45 3 purification purification NOUN dti-1354 45 4 of of ADP dti-1354 45 5 recombinant recombinant ADJ dti-1354 45 6 bmldh bmldh PROPN dti-1354 45 7 in in ADP dti-1354 45 8 e. e. PROPN dti-1354 45 9 coli coli PROPN dti-1354 45 10 bl21 bl21 PROPN dti-1354 45 11 . . PUNCT dti-1354 46 1 both both PRON dti-1354 46 2 bmldh bmldh ADJ dti-1354 46 3 pcr pcr ADJ dti-1354 46 4 product product NOUN dti-1354 46 5 and and CCONJ dti-1354 46 6 pgex6p-1 pgex6p-1 PROPN dti-1354 46 7 expression expression NOUN dti-1354 46 8 vector vector NOUN dti-1354 46 9 were be AUX dti-1354 46 10 double double ADV dti-1354 46 11 digested digest VERB dti-1354 46 12 with with ADP dti-1354 46 13 bamhi bamhi NOUN dti-1354 46 14 ( ( PUNCT dti-1354 46 15 roche roche X dti-1354 46 16 , , PUNCT dti-1354 46 17 germany germany PROPN dti-1354 46 18 ) ) PUNCT dti-1354 46 19 and and CCONJ dti-1354 46 20 xhoi xhoi PROPN dti-1354 46 21 ( ( PUNCT dti-1354 46 22 roche roche NOUN dti-1354 46 23 ) ) PUNCT dti-1354 46 24 restriction restriction NOUN dti-1354 46 25 enzymes enzyme NOUN dti-1354 46 26 . . PUNCT dti-1354 47 1 the the DET dti-1354 47 2 digested digested ADJ dti-1354 47 3 bmldh bmldh NOUN dti-1354 47 4 and and CCONJ dti-1354 47 5 pgex-6p-1 pgex-6p-1 PROPN dti-1354 47 6 were be AUX dti-1354 47 7 ligated ligate VERB dti-1354 47 8 and and CCONJ dti-1354 47 9 transformed transform VERB dti-1354 47 10 into into ADP dti-1354 47 11 competent competent ADJ dti-1354 47 12 e. e. PROPN dti-1354 47 13 coli coli NOUN dti-1354 48 1 bl21 bl21 PROPN dti-1354 48 2 ( ( PUNCT dti-1354 48 3 invitrogen invitrogen PROPN dti-1354 48 4 , , PUNCT dti-1354 48 5 japan japan PROPN dti-1354 48 6 ) ) PUNCT dti-1354 48 7 . . PUNCT dti-1354 49 1 the the DET dti-1354 49 2 nucleotide nucleotide ADJ dti-1354 49 3 sequence sequence NOUN dti-1354 49 4 of of ADP dti-1354 49 5 the the DET dti-1354 49 6 cloned clone VERB dti-1354 49 7 bmldh bmldh PROPN dti-1354 49 8 was be AUX dti-1354 49 9 confirmed confirm VERB dti-1354 49 10 using use VERB dti-1354 49 11 pgex-6p-1 pgex-6p-1 PROPN dti-1354 49 12 forward forward ADV dti-1354 49 13 and and CCONJ dti-1354 49 14 reverse reverse VERB dti-1354 49 15 sequencing sequence VERB dti-1354 49 16 primers primer NOUN dti-1354 49 17 with with ADP dti-1354 49 18 automated automate VERB dti-1354 49 19 sequencer sequencer NOUN dti-1354 49 20 ( ( PUNCT dti-1354 49 21 abi abi PROPN dti-1354 49 22 prism prism NOUN dti-1354 49 23 3100 3100 NUM dti-1354 49 24 genetic genetic ADJ dti-1354 49 25 analyzer analyzer NOUN dti-1354 49 26 , , PUNCT dti-1354 49 27 usa usa PROPN dti-1354 49 28 ) ) PUNCT dti-1354 49 29 . . PUNCT dti-1354 50 1 the the DET dti-1354 50 2 recombinant recombinant ADJ dti-1354 50 3 bmldh bmldh PROPN dti-1354 50 4 was be AUX dti-1354 50 5 expressed express VERB dti-1354 50 6 in in ADP dti-1354 50 7 e. e. PROPN dti-1354 50 8 coli coli NOUN dti-1354 51 1 bl21 bl21 PROPN dti-1354 51 2 as as ADP dti-1354 51 3 glutathione glutathione NOUN dti-1354 51 4 s s NOUN dti-1354 51 5 - - PUNCT dti-1354 51 6 transferase transferase NOUN dti-1354 51 7 fusion fusion NOUN dti-1354 51 8 protein protein NOUN dti-1354 51 9 . . PUNCT dti-1354 52 1 the the DET dti-1354 52 2 soluble soluble ADJ dti-1354 52 3 fraction fraction NOUN dti-1354 52 4 of of ADP dti-1354 52 5 the the DET dti-1354 52 6 recombinant recombinant ADJ dti-1354 52 7 protein protein NOUN dti-1354 52 8 was be AUX dti-1354 52 9 purified purify VERB dti-1354 52 10 as as ADP dti-1354 52 11 previously previously ADV dti-1354 52 12 described21 described21 NOUN dti-1354 52 13 with with ADP dti-1354 52 14 minor minor ADJ dti-1354 52 15 modifications modification NOUN dti-1354 52 16 . . PUNCT dti-1354 53 1 prescission prescission NOUN dti-1354 53 2 ™ ™ ADJ dti-1354 53 3 protease protease NOUN dti-1354 53 4 ( ( PUNCT dti-1354 53 5 ge ge PROPN dti-1354 53 6 healthcare healthcare PROPN dti-1354 53 7 , , PUNCT dti-1354 53 8 sweden sweden PROPN dti-1354 53 9 ) ) PUNCT dti-1354 53 10 was be AUX dti-1354 53 11 used use VERB dti-1354 53 12 to to PART dti-1354 53 13 cleave cleave VERB dti-1354 53 14 bmldh bmldh ADJ dti-1354 53 15 from from ADP dti-1354 53 16 glutathione glutathione NOUN dti-1354 53 17 - - PUNCT dti-1354 53 18 sepharose sepharose NOUN dti-1354 53 19 ™ ™ VERB dti-1354 53 20 4b 4b PROPN dti-1354 53 21 beads bead NOUN dti-1354 53 22 to to PART dti-1354 53 23 yield yield VERB dti-1354 53 24 gst gst NOUN dti-1354 53 25 - - PUNCT dti-1354 53 26 free free ADJ dti-1354 53 27 bmlh bmlh NOUN dti-1354 53 28 for for ADP dti-1354 53 29 enzyme enzyme NOUN dti-1354 53 30 kinetic kinetic NOUN dti-1354 53 31 assay assay NOUN dti-1354 53 32 . . PUNCT dti-1354 54 1 the the DET dti-1354 54 2 purity purity NOUN dti-1354 54 3 and and CCONJ dti-1354 54 4 concentration concentration NOUN dti-1354 54 5 of of ADP dti-1354 54 6 the the DET dti-1354 54 7 purified purified ADJ dti-1354 54 8 protein protein NOUN dti-1354 54 9 were be AUX dti-1354 54 10 determined determine VERB dti-1354 54 11 using use VERB dti-1354 54 12 sodium sodium NOUN dti-1354 54 13 dodecyl dodecyl PROPN dti-1354 54 14 sulfate sulfate NOUN dti-1354 54 15 polyacrylamide polyacrylamide NOUN dti-1354 54 16 gel gel NOUN dti-1354 54 17 electrophoresis electrophoresis NOUN dti-1354 54 18 ( ( PUNCT dti-1354 54 19 sds sds NOUN dti-1354 54 20 - - PUNCT dti-1354 54 21 page page NOUN dti-1354 54 22 ) ) PUNCT dti-1354 54 23 and and CCONJ dti-1354 54 24 lowry lowry NOUN dti-1354 54 25 protein protein NOUN dti-1354 54 26 assay assay NOUN dti-1354 54 27 kit kit NOUN dti-1354 54 28 ( ( PUNCT dti-1354 54 29 thermo thermo NOUN dti-1354 54 30 scientific scientific NOUN dti-1354 54 31 , , PUNCT dti-1354 54 32 usa usa PROPN dti-1354 54 33 ) ) PUNCT dti-1354 54 34 . . PUNCT dti-1354 55 1 production production NOUN dti-1354 55 2 of of ADP dti-1354 55 3 anti anti ADJ dti-1354 55 4 - - ADJ dti-1354 55 5 bmldh bmldh ADJ dti-1354 55 6 sera sera NOUN dti-1354 55 7 . . PUNCT dti-1354 56 1 one one NUM dti-1354 56 2 milliliter milliliter NOUN dti-1354 56 3 of of ADP dti-1354 56 4 the the DET dti-1354 56 5 recombinant recombinant ADJ dti-1354 56 6 bmldh bmldh ADJ dti-1354 56 7 ( ( PUNCT dti-1354 56 8 220 220 NUM dti-1354 56 9     SPACE dti-1354 56 10 µg µg VERB dti-1354 56 11 / / SYM dti-1354 56 12 ml ml VERB dti-1354 56 13 ) ) PUNCT dti-1354 56 14 was be AUX dti-1354 56 15 mixed mix VERB dti-1354 56 16 with with ADP dti-1354 56 17 1 1 NUM dti-1354 56 18     SPACE dti-1354 56 19 ml ml NOUN dti-1354 56 20 of of ADP dti-1354 56 21 complete complete ADJ dti-1354 56 22 freund freund PROPN dti-1354 56 23 ’s ’s PART dti-1354 56 24 adjuvant adjuvant NOUN dti-1354 56 25 ( ( PUNCT dti-1354 56 26 cfa cfa NOUN dti-1354 56 27 ) ) PUNCT dti-1354 56 28 in in ADP dti-1354 56 29 sterile sterile ADJ dti-1354 56 30 syringe syringe NOUN dti-1354 56 31 as as SCONJ dti-1354 56 32 previously previously ADV dti-1354 56 33 described.22 described.22 VERB dti-1354 56 34 five five NUM dti-1354 56 35 balb balb ADJ dti-1354 56 36 / / SYM dti-1354 56 37 c c NOUN dti-1354 56 38 mice mouse NOUN dti-1354 56 39 ( ( PUNCT dti-1354 56 40 10 10 NUM dti-1354 56 41 weeks week NOUN dti-1354 56 42 old old ADJ dti-1354 56 43 ) ) PUNCT dti-1354 56 44 were be AUX dti-1354 56 45 immunized immunize VERB dti-1354 56 46 with with ADP dti-1354 56 47 33 33 NUM dti-1354 56 48     SPACE dti-1354 56 49 ng ng PROPN dti-1354 56 50 of of ADP dti-1354 56 51 bmldh bmldh PROPN dti-1354 56 52 through through ADP dti-1354 56 53 the the DET dti-1354 56 54 intra intra ADJ dti-1354 56 55 - - ADJ dti-1354 56 56 peritoneal peritoneal ADJ dti-1354 56 57 route route NOUN dti-1354 56 58 . . PUNCT dti-1354 57 1 three three NUM dti-1354 57 2 booster booster NOUN dti-1354 57 3 doses dose NOUN dti-1354 57 4 of of ADP dti-1354 57 5 15 15 NUM dti-1354 57 6 days day NOUN dti-1354 57 7 apart apart ADV dti-1354 57 8 were be AUX dti-1354 57 9 administered administer VERB dti-1354 57 10 , , PUNCT dti-1354 57 11 and and CCONJ dti-1354 57 12 sera sera NOUN dti-1354 57 13 were be AUX dti-1354 57 14 collected collect VERB dti-1354 57 15 10 10 NUM dti-1354 57 16 days day NOUN dti-1354 57 17 after after ADP dti-1354 57 18 the the DET dti-1354 57 19 last last ADJ dti-1354 57 20 dose dose NOUN dti-1354 57 21 . . PUNCT dti-1354 58 1 infection infection NOUN dti-1354 58 2 of of ADP dti-1354 58 3 hamsters hamster NOUN dti-1354 58 4 with with ADP dti-1354 58 5 b. b. PROPN dti-1354 58 6 microti microti PROPN dti-1354 58 7 gray gray PROPN dti-1354 58 8 strain strain NOUN dti-1354 58 9 , , PUNCT dti-1354 58 10 parasite parasite NOUN dti-1354 58 11 lysate lysate NOUN dti-1354 58 12 , , PUNCT dti-1354 58 13 and and CCONJ dti-1354 58 14 western western ADJ dti-1354 58 15 blotting blotting NOUN dti-1354 58 16 . . PUNCT dti-1354 59 1 four four NUM dti-1354 59 2 female female ADJ dti-1354 59 3 ( ( PUNCT dti-1354 59 4 8 8 NUM dti-1354 59 5 weeks week NOUN dti-1354 59 6 old old ADJ dti-1354 59 7 ) ) PUNCT dti-1354 59 8 specific specific ADJ dti-1354 59 9 pathogen pathogen NOUN dti-1354 59 10 - - PUNCT dti-1354 59 11 free free ADJ dti-1354 59 12 ( ( PUNCT dti-1354 59 13 spf spf ADJ dti-1354 59 14 ) ) PUNCT dti-1354 59 15 syrian syrian ADJ dti-1354 59 16 hamsters hamster NOUN dti-1354 59 17 ( ( PUNCT dti-1354 59 18 slc slc PROPN dti-1354 59 19 , , PUNCT dti-1354 59 20 japan japan PROPN dti-1354 59 21 ) ) PUNCT dti-1354 59 22 were be AUX dti-1354 59 23 used use VERB dti-1354 59 24 in in ADP dti-1354 59 25 this this DET dti-1354 59 26 experiment experiment NOUN dti-1354 59 27 . . PUNCT dti-1354 60 1 prior prior ADV dti-1354 60 2 to to ADP dti-1354 60 3 parasite parasite ADJ dti-1354 60 4 inoculation inoculation NOUN dti-1354 60 5 , , PUNCT dti-1354 60 6 spf spf ADJ dti-1354 60 7 sera sera NOUN dti-1354 60 8 were be AUX dti-1354 60 9 obtained obtain VERB dti-1354 60 10 from from ADP dti-1354 60 11 all all DET dti-1354 60 12 the the DET dti-1354 60 13 hamsters hamster NOUN dti-1354 60 14 . . PUNCT dti-1354 61 1 two two NUM dti-1354 61 2 hamsters hamster NOUN dti-1354 61 3 were be AUX dti-1354 61 4 inoculated inoculate VERB dti-1354 61 5 with with ADP dti-1354 61 6 previously previously ADV dti-1354 61 7 stocked stock VERB dti-1354 61 8 b. b. PROPN dti-1354 61 9 microti microti PROPN dti-1354 61 10 - - PUNCT dti-1354 61 11 infected infect VERB dti-1354 61 12 hamster hamster PROPN dti-1354 61 13 rbc rbc PROPN dti-1354 61 14 intra intra PROPN dti-1354 61 15 - - ADJ dti-1354 61 16 peritoneally peritoneally ADV dti-1354 61 17 as as SCONJ dti-1354 61 18 previously previously ADV dti-1354 61 19 described.23 described.23 VERB dti-1354 61 20 observation observation NOUN dti-1354 61 21 of of ADP dti-1354 61 22 parasitemia parasitemia NOUN dti-1354 61 23 was be AUX dti-1354 61 24 done do VERB dti-1354 61 25 every every DET dti-1354 61 26 2 2 NUM dti-1354 61 27 days day NOUN dti-1354 61 28 by by ADP dti-1354 61 29 microscopy microscopy NOUN dti-1354 61 30 . . PUNCT dti-1354 62 1 at at ADP dti-1354 62 2 peak peak NOUN dti-1354 62 3 ( ( PUNCT dti-1354 62 4 51 51 NUM dti-1354 62 5 % % NOUN dti-1354 62 6 ) ) PUNCT dti-1354 62 7 parasitemia parasitemia NOUN dti-1354 62 8 ( ( PUNCT dti-1354 62 9 day day NOUN dti-1354 62 10 13 13 NUM dti-1354 62 11 and and CCONJ dti-1354 62 12 14 14 NUM dti-1354 62 13 ) ) PUNCT dti-1354 62 14 , , PUNCT dti-1354 62 15 the the DET dti-1354 62 16 hamsters hamster NOUN dti-1354 62 17 were be AUX dti-1354 62 18 sacrificed sacrifice VERB dti-1354 62 19 , , PUNCT dti-1354 62 20 and and CCONJ dti-1354 62 21 blood blood NOUN dti-1354 62 22 was be AUX dti-1354 62 23 obtained obtain VERB dti-1354 62 24 for for ADP dti-1354 62 25 collection collection NOUN dti-1354 62 26 of of ADP dti-1354 62 27 infected infected ADJ dti-1354 62 28 sera sera NOUN dti-1354 62 29 and and CCONJ dti-1354 62 30 preparation preparation NOUN dti-1354 62 31 of of ADP dti-1354 62 32 parasite parasite ADJ dti-1354 62 33 lysate lysate NOUN dti-1354 62 34 . . PUNCT dti-1354 63 1 parasite parasite NOUN dti-1354 63 2 and and CCONJ dti-1354 63 3 non non ADJ dti-1354 63 4 - - ADJ dti-1354 63 5 infected infected ADJ dti-1354 63 6 rbc rbc PROPN dti-1354 63 7 lysates lysate NOUN dti-1354 63 8 were be AUX dti-1354 63 9 prepared prepare VERB dti-1354 63 10 as as SCONJ dti-1354 63 11 previously previously ADV dti-1354 63 12 described.24 described.24 ADJ dti-1354 63 13 transfer transfer NOUN dti-1354 63 14 of of ADP dti-1354 63 15 the the DET dti-1354 63 16 protein protein NOUN dti-1354 63 17 from from ADP dti-1354 63 18 the the DET dti-1354 63 19 gel gel NOUN dti-1354 63 20 to to ADP dti-1354 63 21 western western ADJ dti-1354 63 22 blot blot NOUN dti-1354 63 23 membrane membrane NOUN dti-1354 63 24 was be AUX dti-1354 63 25 carried carry VERB dti-1354 63 26 out out ADP dti-1354 63 27 as as ADP dti-1354 63 28 previously previously ADV dti-1354 63 29 described.21 described.21 PROPN dti-1354 63 30 however however ADV dti-1354 63 31 , , PUNCT dti-1354 63 32 the the DET dti-1354 63 33 bmldh bmldh ADJ dti-1354 63 34 polyclonal polyclonal ADJ dti-1354 63 35 sera sera NOUN dti-1354 63 36 was be AUX dti-1354 63 37 diluted dilute VERB dti-1354 63 38 at at ADP dti-1354 63 39 1:250 1:250 NUM dti-1354 63 40 , , PUNCT dti-1354 63 41 and and CCONJ dti-1354 63 42 the the DET dti-1354 63 43 antibody antibody NOUN dti-1354 63 44 solution solution NOUN dti-1354 63 45 was be AUX dti-1354 63 46 diluted dilute VERB dti-1354 63 47 at at ADP dti-1354 63 48 1:2,000 1:2,000 PROPN dti-1354 63 49 . . PUNCT dti-1354 64 1 the the DET dti-1354 64 2 native native ADJ dti-1354 64 3 bmldh bmldh PROPN dti-1354 64 4 was be AUX dti-1354 64 5 detected detect VERB dti-1354 64 6 with with ADP dti-1354 64 7 color color NOUN dti-1354 64 8 development development NOUN dti-1354 64 9 solution solution NOUN dti-1354 64 10 containing contain VERB dti-1354 64 11 3,3′-diaminobenzidine 3,3′-diaminobenzidine NUM dti-1354 64 12 tetrahydrochloride tetrahydrochloride NOUN dti-1354 64 13 ( ( PUNCT dti-1354 64 14 dab dab PROPN dti-1354 64 15 ) ) PUNCT dti-1354 64 16 and and CCONJ dti-1354 64 17 hydrogen hydrogen NOUN dti-1354 64 18 peroxide peroxide NOUN dti-1354 64 19 . . PUNCT dti-1354 65 1 native native ADJ dti-1354 65 2 protein protein NOUN dti-1354 65 3 localization localization NOUN dti-1354 65 4 by by ADP dti-1354 65 5 indirect indirect ADJ dti-1354 65 6 florescent florescent NOUN dti-1354 65 7 antibody antibody NOUN dti-1354 65 8 test test NOUN dti-1354 65 9 ( ( PUNCT dti-1354 65 10 ifat ifat NOUN dti-1354 65 11 ) ) PUNCT dti-1354 65 12 and and CCONJ dti-1354 65 13 confocal confocal ADJ dti-1354 65 14 laser laser NOUN dti-1354 65 15 microscopy microscopy NOUN dti-1354 65 16 . . PUNCT dti-1354 66 1 this this PRON dti-1354 66 2 was be AUX dti-1354 66 3 carried carry VERB dti-1354 66 4 out out ADP dti-1354 66 5 using use VERB dti-1354 66 6 b. b. PROPN dti-1354 66 7 microti microti PROPN dti-1354 66 8 gray gray ADJ dti-1354 66 9 strain strain NOUN dti-1354 66 10 - - PUNCT dti-1354 66 11 infected infect VERB dti-1354 66 12 hamster hamster NOUN dti-1354 66 13 blood blood NOUN dti-1354 66 14 smears smear NOUN dti-1354 66 15 ( ( PUNCT dti-1354 66 16 day day NOUN dti-1354 66 17 8 8 NUM dti-1354 66 18 post post ADJ dti-1354 66 19 - - NOUN dti-1354 66 20 infection infection NOUN dti-1354 66 21 ) ) PUNCT dti-1354 66 22 as as SCONJ dti-1354 66 23 previously previously ADV dti-1354 66 24 described.21 described.21 VERB dti-1354 66 25 the the DET dti-1354 66 26 secondary secondary ADJ dti-1354 66 27 antibody antibody NOUN dti-1354 66 28 was be AUX dti-1354 66 29 anti anti ADJ dti-1354 66 30 - - ADJ dti-1354 66 31 mouse mouse ADJ dti-1354 66 32 alexa-488 alexa-488 ADJ dti-1354 66 33 secondary secondary ADJ dti-1354 66 34 diluted dilute VERB dti-1354 66 35 200 200 NUM dti-1354 66 36 times time NOUN dti-1354 66 37 with with ADP dti-1354 66 38 4 4 NUM dti-1354 66 39 % % NOUN dti-1354 66 40 fetal fetal ADJ dti-1354 66 41 bovine bovine NOUN dti-1354 66 42 serum serum NOUN dti-1354 66 43 ( ( PUNCT dti-1354 66 44 fbs fbs PROPN dti-1354 66 45 ) ) PUNCT dti-1354 66 46 , , PUNCT dti-1354 66 47 and and CCONJ dti-1354 66 48 the the DET dti-1354 66 49 http://www.la-press.com http://www.la-press.com X dti-1354 66 50 molecular molecular ADJ dti-1354 66 51 and and CCONJ dti-1354 66 52 kinetic kinetic ADJ dti-1354 66 53 characterization characterization NOUN dti-1354 66 54 of of ADP dti-1354 66 55 b. b. PROPN dti-1354 66 56 microti microti PROPN dti-1354 66 57 ldh ldh PROPN dti-1354 66 58 33drug 33drug NUM dti-1354 66 59 target target NOUN dti-1354 66 60 insights insight NOUN dti-1354 66 61 2014:8 2014:8 NUM dti-1354 66 62 parasite parasite NOUN dti-1354 66 63 nucleus nucleus NOUN dti-1354 66 64 was be AUX dti-1354 66 65 stained stain VERB dti-1354 66 66 with with ADP dti-1354 66 67 propidium propidium NOUN dti-1354 66 68 iodide iodide NOUN dti-1354 66 69 . . PUNCT dti-1354 67 1 parasites parasite NOUN dti-1354 67 2 were be AUX dti-1354 67 3 observed observe VERB dti-1354 67 4 using use VERB dti-1354 67 5 confocal confocal ADJ dti-1354 67 6 laser laser NOUN dti-1354 67 7 scanning scan VERB dti-1354 67 8 microscope microscope NOUN dti-1354 67 9 ( ( PUNCT dti-1354 67 10 leicia leicia PROPN dti-1354 67 11 , , PUNCT dti-1354 67 12 japan japan PROPN dti-1354 67 13 ) ) PUNCT dti-1354 67 14 . . PUNCT dti-1354 68 1 enzyme enzyme NOUN dti-1354 68 2 kinetic kinetic ADJ dti-1354 68 3 assay assay NOUN dti-1354 68 4 . . PUNCT dti-1354 69 1 optimization optimization NOUN dti-1354 69 2 of of ADP dti-1354 69 3 ph ph NOUN dti-1354 69 4 and and CCONJ dti-1354 69 5 determination determination NOUN dti-1354 69 6 of of ADP dti-1354 69 7 vmax vmax PROPN dti-1354 69 8 and and CCONJ dti-1354 69 9 km km PROPN dti-1354 69 10 for for ADP dti-1354 69 11 lactate lactate NOUN dti-1354 69 12 and and CCONJ dti-1354 69 13 nad+ nad+ PROPN dti-1354 69 14 . . PUNCT dti-1354 70 1 the the DET dti-1354 70 2 method method NOUN dti-1354 70 3 by by ADP dti-1354 70 4 kavanagh kavanagh PROPN dti-1354 70 5 and and CCONJ dti-1354 70 6 others other NOUN dti-1354 70 7 was be AUX dti-1354 70 8 used use VERB dti-1354 70 9 with with ADP dti-1354 70 10 minor minor ADJ dti-1354 70 11 modifications.16 modifications.16 NOUN dti-1354 70 12 the the DET dti-1354 70 13 oxidation oxidation NOUN dti-1354 70 14 of of ADP dti-1354 70 15 lactate lactate NOUN dti-1354 70 16 to to PART dti-1354 70 17 pyruvate pyruvate VERB dti-1354 70 18 and and CCONJ dti-1354 70 19 subsequent subsequent ADJ dti-1354 70 20 formation formation NOUN dti-1354 70 21 of of ADP dti-1354 70 22 nadh nadh NOUN dti-1354 70 23 from from ADP dti-1354 70 24 nad+ nad+ PROPN dti-1354 70 25 was be AUX dti-1354 70 26 assayed assayed ADJ dti-1354 70 27 in in ADP dti-1354 70 28 7 7 NUM dti-1354 70 29     SPACE dti-1354 70 30 mm mm PROPN dti-1354 70 31 kcl kcl PROPN dti-1354 70 32 and and CCONJ dti-1354 70 33 70 70 NUM dti-1354 70 34     SPACE dti-1354 70 35 mm mm PROPN dti-1354 70 36 tris tris PROPN dti-1354 70 37 - - PUNCT dti-1354 70 38 hcl hcl NOUN dti-1354 70 39 buffer buffer NOUN dti-1354 70 40 . . PUNCT dti-1354 71 1 optimal optimal ADJ dti-1354 71 2 ph ph VERB dti-1354 71 3 , , PUNCT dti-1354 71 4 km km PROPN dti-1354 71 5 , , PUNCT dti-1354 71 6 and and CCONJ dti-1354 71 7 vmax vmax PROPN dti-1354 71 8 were be AUX dti-1354 71 9 all all PRON dti-1354 71 10 determined determined ADJ dti-1354 71 11 using use VERB dti-1354 71 12 0.5 0.5 NUM dti-1354 71 13     SPACE dti-1354 71 14 µg µg NOUN dti-1354 71 15 of of ADP dti-1354 71 16 bmldh bmldh ADJ dti-1354 71 17 . . PUNCT dti-1354 72 1 the the DET dti-1354 72 2 amount amount NOUN dti-1354 72 3 of of ADP dti-1354 72 4 nadh nadh NOUN dti-1354 72 5 produced produce VERB dti-1354 72 6 was be AUX dti-1354 72 7 measured measure VERB dti-1354 72 8 using use VERB dti-1354 72 9 du du PROPN dti-1354 72 10 ® ® NOUN dti-1354 72 11 800 800 NUM dti-1354 72 12 spectrophotometer spectrophotometer NOUN dti-1354 72 13 ( ( PUNCT dti-1354 72 14 beckman beckman PROPN dti-1354 72 15 coulter coulter PROPN dti-1354 72 16 inc inc PROPN dti-1354 72 17 . . PROPN dti-1354 72 18 , , PUNCT dti-1354 72 19 fullerton fullerton PROPN dti-1354 72 20 , , PUNCT dti-1354 72 21 ca ca PROPN dti-1354 72 22 , , PUNCT dti-1354 72 23 usa usa PROPN dti-1354 72 24 ) ) PUNCT dti-1354 72 25 at at ADP dti-1354 72 26 340 340 NUM dti-1354 72 27     SPACE dti-1354 72 28 nm nm NOUN dti-1354 72 29 ( ( PUNCT dti-1354 72 30 ε ε PROPN dti-1354 72 31     SPACE dti-1354 72 32 = = NOUN dti-1354 72 33     SPACE dti-1354 72 34 6.2 6.2 NUM dti-1354 72 35     SPACE dti-1354 72 36 mm-1 mm-1 NOUN dti-1354 72 37     SPACE dti-1354 72 38 cm-1 cm-1 NOUN dti-1354 72 39 ) ) PUNCT dti-1354 72 40 and and CCONJ dti-1354 72 41 25 25 NUM dti-1354 72 42 ° ° NOUN dti-1354 72 43 c c NOUN dti-1354 72 44 . . PUNCT dti-1354 73 1 optimal optimal ADJ dti-1354 73 2 ph ph NOUN dti-1354 73 3 for for ADP dti-1354 73 4 bmldh bmldh ADJ dti-1354 73 5 catalytic catalytic ADJ dti-1354 73 6 activity activity NOUN dti-1354 73 7 was be AUX dti-1354 73 8 determined determine VERB dti-1354 73 9 using use VERB dti-1354 73 10 3 3 NUM dti-1354 73 11     SPACE dti-1354 73 12 mm mm PROPN dti-1354 73 13 nad+ nad+ PROPN dti-1354 73 14 and and CCONJ dti-1354 73 15 120 120 NUM dti-1354 73 16     SPACE dti-1354 73 17 mm mm NOUN dti-1354 73 18 lactate lactate NOUN dti-1354 73 19 in in ADP dti-1354 73 20 varying vary VERB dti-1354 73 21 ph ph NOUN dti-1354 73 22 ( ( PUNCT dti-1354 73 23 2–12.2 2–12.2 NUM dti-1354 73 24 ) ) PUNCT dti-1354 73 25 of of ADP dti-1354 73 26 the the DET dti-1354 73 27 buffer buffer NOUN dti-1354 73 28 . . PUNCT dti-1354 74 1 initial initial ADJ dti-1354 74 2 velocities velocity NOUN dti-1354 74 3 for for ADP dti-1354 74 4 the the DET dti-1354 74 5 rate rate NOUN dti-1354 74 6 of of ADP dti-1354 74 7 formation formation NOUN dti-1354 74 8 of of ADP dti-1354 74 9 nadh nadh NOUN dti-1354 74 10 in in ADP dti-1354 74 11 the the DET dti-1354 74 12 first first ADJ dti-1354 74 13 60 60 NUM dti-1354 74 14 seconds second NOUN dti-1354 74 15 were be AUX dti-1354 74 16 calculated calculate VERB dti-1354 74 17 , , PUNCT dti-1354 74 18 and and CCONJ dti-1354 74 19 relative relative ADJ dti-1354 74 20 percentage percentage NOUN dti-1354 74 21 activity activity NOUN dti-1354 74 22 was be AUX dti-1354 74 23 determined determine VERB dti-1354 74 24 . . PUNCT dti-1354 75 1 the the DET dti-1354 75 2 km km NOUN dti-1354 75 3 and and CCONJ dti-1354 75 4 vmax vmax PROPN dti-1354 75 5 for for ADP dti-1354 75 6 nad+ nad+ PROPN dti-1354 75 7 ( ( PUNCT dti-1354 75 8 co co NOUN dti-1354 75 9 - - NOUN dti-1354 75 10 factor factor NOUN dti-1354 75 11 ) ) PUNCT dti-1354 75 12 were be AUX dti-1354 75 13 determined determine VERB dti-1354 75 14 in in ADP dti-1354 75 15 a a DET dti-1354 75 16 500 500 NUM dti-1354 75 17     SPACE dti-1354 75 18 µl µl ADP dti-1354 75 19 reaction reaction NOUN dti-1354 75 20 volume volume NOUN dti-1354 75 21 containing contain VERB dti-1354 75 22 the the DET dti-1354 75 23 buffer buffer NOUN dti-1354 75 24 , , PUNCT dti-1354 75 25 100 100 NUM dti-1354 75 26     SPACE dti-1354 75 27 mm mm PROPN dti-1354 75 28 lactate lactate NOUN dti-1354 75 29 and and CCONJ dti-1354 75 30 0.05–6.8 0.05–6.8 NUM dti-1354 75 31     SPACE dti-1354 75 32 mm mm PROPN dti-1354 75 33 nad+ nad+ PROPN dti-1354 75 34 . . PUNCT dti-1354 76 1 similarly similarly ADV dti-1354 76 2 , , PUNCT dti-1354 76 3 km km PROPN dti-1354 76 4 and and CCONJ dti-1354 76 5 vmax vmax PROPN dti-1354 76 6 for for ADP dti-1354 76 7 lactate lactate NOUN dti-1354 76 8 were be AUX dti-1354 76 9 assayed assayed ADJ dti-1354 76 10 in in ADP dti-1354 76 11 a a DET dti-1354 76 12 solution solution NOUN dti-1354 76 13 containing contain VERB dti-1354 76 14 the the DET dti-1354 76 15 buffer buffer NOUN dti-1354 76 16 , , PUNCT dti-1354 76 17 2.4 2.4 NUM dti-1354 76 18     SPACE dti-1354 76 19 mm mm PROPN dti-1354 76 20 nad+ nad+ PROPN dti-1354 76 21 and and CCONJ dti-1354 76 22 20–500 20–500 NUM dti-1354 76 23     SPACE dti-1354 76 24 mm mm NOUN dti-1354 76 25 lactate lactate NOUN dti-1354 76 26 . . PUNCT dti-1354 77 1 bmldh bmldh ADJ dti-1354 77 2 inhibitory inhibitory ADJ dti-1354 77 3 assay assay NOUN dti-1354 77 4 . . PUNCT dti-1354 78 1 this this PRON dti-1354 78 2 was be AUX dti-1354 78 3 carried carry VERB dti-1354 78 4 out out ADP dti-1354 78 5 according accord VERB dti-1354 78 6 to to ADP dti-1354 78 7 the the DET dti-1354 78 8 methods method NOUN dti-1354 78 9 reported report VERB dti-1354 78 10 by by ADP dti-1354 78 11 bork bork NOUN dti-1354 78 12 and and CCONJ dti-1354 78 13 colleagues,19 colleagues,19 NOUN dti-1354 78 14 with with ADP dti-1354 78 15 gossypol gossypol PROPN dti-1354 78 16 as as ADP dti-1354 78 17 an an DET dti-1354 78 18 inhibitor inhibitor NOUN dti-1354 78 19 . . PUNCT dti-1354 79 1 briefly briefly ADV dti-1354 79 2 , , PUNCT dti-1354 79 3 a a DET dti-1354 79 4 400 400 NUM dti-1354 79 5     SPACE dti-1354 79 6 mm mm PROPN dti-1354 79 7 gossypol gossypol PROPN dti-1354 79 8 stock stock NOUN dti-1354 79 9 solution solution NOUN dti-1354 79 10 was be AUX dti-1354 79 11 prepared prepare VERB dti-1354 79 12 by by ADP dti-1354 79 13 dissolving dissolve VERB dti-1354 79 14 gossypol gossypol PROPN dti-1354 79 15 in in ADP dti-1354 79 16 dimethyl dimethyl NOUN dti-1354 79 17 sulfoxide sulfoxide NOUN dti-1354 79 18 ( ( PUNCT dti-1354 79 19 dmso dmso NOUN dti-1354 79 20 ) ) PUNCT dti-1354 79 21 . . PUNCT dti-1354 80 1 the the DET dti-1354 80 2 percentage percentage NOUN dti-1354 80 3 inhibition inhibition NOUN dti-1354 80 4 of of ADP dti-1354 80 5 bmldh bmldh ADJ dti-1354 80 6 was be AUX dti-1354 80 7 determined determine VERB dti-1354 80 8 at at ADP dti-1354 80 9 various various ADJ dti-1354 80 10 concentration concentration NOUN dti-1354 80 11 of of ADP dti-1354 80 12 gossypol gossypol PROPN dti-1354 80 13 ( ( PUNCT dti-1354 80 14 9.5–5,000 9.5–5,000 NUM dti-1354 80 15     SPACE dti-1354 80 16 nm nm NOUN dti-1354 80 17 ) ) PUNCT dti-1354 80 18 at at ADP dti-1354 80 19 1.2 1.2 NUM dti-1354 80 20 and and CCONJ dti-1354 80 21 30 30 NUM dti-1354 80 22     SPACE dti-1354 80 23 mm mm NOUN dti-1354 80 24 of of ADP dti-1354 80 25 nad+ nad+ PROPN dti-1354 80 26 and and CCONJ dti-1354 80 27 lactate lactate PROPN dti-1354 80 28 , , PUNCT dti-1354 80 29 respectively respectively ADV dti-1354 80 30 . . PUNCT dti-1354 81 1 the the DET dti-1354 81 2 final final ADJ dti-1354 81 3 concentration concentration NOUN dti-1354 81 4 of of ADP dti-1354 81 5 dmso dmso NOUN dti-1354 81 6 in in ADP dti-1354 81 7 each each DET dti-1354 81 8 assay assay NOUN dti-1354 81 9 did do AUX dti-1354 81 10 not not PART dti-1354 81 11 exceed exceed VERB dti-1354 81 12 0.2 0.2 NUM dti-1354 81 13 % % NOUN dti-1354 81 14 . . PUNCT dti-1354 82 1 the the DET dti-1354 82 2 negative negative ADJ dti-1354 82 3 control control NOUN dti-1354 82 4 contained contain VERB dti-1354 82 5 0.2 0.2 NUM dti-1354 82 6 % % NOUN dti-1354 82 7 of of ADP dti-1354 82 8 dmso dmso NOUN dti-1354 82 9 . . PUNCT dti-1354 83 1 all all DET dti-1354 83 2 the the DET dti-1354 83 3 experiments experiment NOUN dti-1354 83 4 were be AUX dti-1354 83 5 repeated repeat VERB dti-1354 83 6 three three NUM dti-1354 83 7 times time NOUN dti-1354 83 8 , , PUNCT dti-1354 83 9 and and CCONJ dti-1354 83 10 the the DET dti-1354 83 11 concentration concentration NOUN dti-1354 83 12 of of ADP dti-1354 83 13 gossypol gossypol PROPN dti-1354 83 14 that that PRON dti-1354 83 15 inhibited inhibit VERB dti-1354 83 16 nadh nadh NOUN dti-1354 83 17 production production NOUN dti-1354 83 18 by by ADP dti-1354 83 19 50 50 NUM dti-1354 83 20 % % NOUN dti-1354 83 21 ( ( PUNCT dti-1354 83 22 ic50 ic50 PROPN dti-1354 83 23 ) ) PUNCT dti-1354 83 24 was be AUX dti-1354 83 25 calculated calculate VERB dti-1354 83 26 using use VERB dti-1354 83 27 non non ADJ dti-1354 83 28 - - ADJ dti-1354 83 29 linear linear ADJ dti-1354 83 30 regression regression NOUN dti-1354 83 31 with with ADP dti-1354 83 32 graphpad graphpad NOUN dti-1354 83 33 prism prism NOUN dti-1354 83 34 ( ( PUNCT dti-1354 83 35 graphpad graphpad NOUN dti-1354 83 36 prism prism NOUN dti-1354 83 37 version version NOUN dti-1354 83 38 5.00 5.00 NUM dti-1354 83 39 for for ADP dti-1354 83 40 windows window NOUN dti-1354 83 41 , , PUNCT dti-1354 83 42 graphpad graphpad NOUN dti-1354 83 43 software software NOUN dti-1354 83 44 , , PUNCT dti-1354 83 45 usa usa PROPN dti-1354 83 46 ) ) PUNCT dti-1354 83 47 . . PUNCT dti-1354 84 1 bioinformatic bioinformatic ADJ dti-1354 84 2 analysis analysis NOUN dti-1354 84 3 . . PUNCT dti-1354 85 1 the the DET dti-1354 85 2 bmldh bmldh ADJ dti-1354 85 3 nucleotide nucleotide NOUN dti-1354 85 4 sequenc sequenc PROPN dti-1354 85 5 es es PROPN dti-1354 85 6 of of ADP dti-1354 85 7 both both DET dti-1354 85 8 pgem pgem PROPN dti-1354 85 9 - - PUNCT dti-1354 85 10 t t NOUN dti-1354 85 11 easy easy ADV dti-1354 85 12 - - PUNCT dti-1354 85 13 bmldh bmldh ADJ dti-1354 85 14 and and CCONJ dti-1354 85 15 pgex-6p-1 pgex-6p-1 PROPN dti-1354 85 16 - - PUNCT dti-1354 85 17 bmldh bmldh ADJ dti-1354 85 18 clones clone NOUN dti-1354 85 19 were be AUX dti-1354 85 20 edited edit VERB dti-1354 85 21 using use VERB dti-1354 85 22 codoncode codoncode PROPN dti-1354 85 23 aligner aligner PROPN dti-1354 85 24 version version NOUN dti-1354 85 25 1.5.2 1.5.2 NUM dti-1354 85 26 ( ( PUNCT dti-1354 85 27 codoncode codoncode PROPN dti-1354 85 28 corporation corporation PROPN dti-1354 85 29 , , PUNCT dti-1354 85 30 usa usa PROPN dti-1354 85 31 ) ) PUNCT dti-1354 85 32 and and CCONJ dti-1354 85 33 consensus consensus NOUN dti-1354 85 34 sequence sequence NOUN dti-1354 85 35 derived derive VERB dti-1354 85 36 using use VERB dti-1354 85 37 genetyx genetyx NOUN dti-1354 85 38 software software NOUN dti-1354 85 39 ( ( PUNCT dti-1354 85 40 genetyx genetyx PROPN dti-1354 85 41 corporation corporation NOUN dti-1354 85 42 , , PUNCT dti-1354 85 43 japan japan PROPN dti-1354 85 44 ) ) PUNCT dti-1354 85 45 . . PUNCT dti-1354 86 1 the the DET dti-1354 86 2 consensus consensus NOUN dti-1354 86 3 nucleotide nucleotide NOUN dti-1354 86 4 sequences sequence NOUN dti-1354 86 5 for for ADP dti-1354 86 6 full full ADJ dti-1354 86 7 - - PUNCT dti-1354 86 8 length length NOUN dti-1354 86 9 bmldh bmldh PROPN dti-1354 86 10 were be AUX dti-1354 86 11 aligned align VERB dti-1354 86 12 using use VERB dti-1354 86 13 blast blast NOUN dti-1354 86 14 ( ( PUNCT dti-1354 86 15 http://blast.ncbi.nlm http://blast.ncbi.nlm PROPN dti-1354 86 16 . . PUNCT dti-1354 87 1 nih.gov/ nih.gov/ NOUN dti-1354 87 2 ) ) PUNCT dti-1354 88 1 to to PART dti-1354 88 2 determine determine VERB dti-1354 88 3 the the DET dti-1354 88 4 homology homology NOUN dti-1354 88 5 . . PUNCT dti-1354 89 1 prediction prediction NOUN dti-1354 89 2 of of ADP dti-1354 89 3 importin importin NOUN dti-1354 89 4 α α NUM dti-1354 89 5 - - ADJ dti-1354 89 6 dependent dependent ADJ dti-1354 89 7 nuclear nuclear ADJ dti-1354 89 8 localization localization NOUN dti-1354 89 9 signals signal NOUN dti-1354 89 10 was be AUX dti-1354 89 11 done do VERB dti-1354 89 12 using use VERB dti-1354 89 13 cytoplasmic cytoplasmic ADJ dti-1354 89 14 - - PUNCT dti-1354 89 15 nucleus nucleus NOUN dti-1354 89 16 localization localization NOUN dti-1354 89 17 signal signal NOUN dti-1354 89 18 predictor predictor NOUN dti-1354 89 19 ( ( PUNCT dti-1354 89 20 cnls cnls PROPN dti-1354 89 21 ) ) PUNCT dti-1354 89 22 mapper mapper NOUN dti-1354 89 23 so so SCONJ dti-1354 89 24 as as SCONJ dti-1354 89 25 to to PART dti-1354 89 26 account account VERB dti-1354 89 27 for for ADP dti-1354 89 28 localization localization NOUN dti-1354 89 29 of of ADP dti-1354 89 30 the the DET dti-1354 89 31 native native ADJ dti-1354 89 32 protein protein NOUN dti-1354 89 33 based base VERB dti-1354 89 34 on on ADP dti-1354 89 35 its its PRON dti-1354 89 36 amino amino NOUN dti-1354 89 37 acid acid NOUN dti-1354 89 38 sequence sequence NOUN dti-1354 89 39 ( ( PUNCT dti-1354 89 40 http://nls-mapper.iab.keio.ac.jp/cgibin/nls_mapper_form.cgi http://nls-mapper.iab.keio.ac.jp/cgibin/nls_mapper_form.cgi PROPN dti-1354 89 41 ) ) PUNCT dti-1354 89 42 . . PUNCT dti-1354 90 1 results result VERB dti-1354 90 2 characterization characterization NOUN dti-1354 90 3 of of ADP dti-1354 90 4 bmldh bmldh ADJ dti-1354 90 5 gene gene NOUN dti-1354 90 6 . . PUNCT dti-1354 91 1 the the DET dti-1354 91 2 amplified amplify VERB dti-1354 91 3 bmldh bmldh ADJ dti-1354 91 4 gene gene NOUN dti-1354 91 5 was be AUX dti-1354 91 6 approximately approximately ADV dti-1354 91 7 0.99 0.99 NUM dti-1354 91 8     SPACE dti-1354 91 9 kbp kbp PROPN dti-1354 91 10 . . PROPN dti-1354 91 11 nucleotide nucleotide PROPN dti-1354 91 12 sequence sequence NOUN dti-1354 91 13 analysis analysis NOUN dti-1354 91 14 revealed reveal VERB dti-1354 91 15 that that SCONJ dti-1354 91 16 bmldh bmldh ADJ dti-1354 91 17 gene gene NOUN dti-1354 91 18 has have VERB dti-1354 91 19 an an DET dti-1354 91 20 open open ADJ dti-1354 91 21 reading reading NOUN dti-1354 91 22 frame frame NOUN dti-1354 91 23 of of ADP dti-1354 91 24 996 996 NUM dti-1354 91 25 nucleotides nucleotide NOUN dti-1354 91 26 that that PRON dti-1354 91 27 codes code VERB dti-1354 91 28 for for ADP dti-1354 91 29 332 332 NUM dti-1354 91 30 amino amino ADJ dti-1354 91 31 acids acid NOUN dti-1354 91 32 . . PUNCT dti-1354 92 1 there there PRON dti-1354 92 2 were be VERB dti-1354 92 3 no no DET dti-1354 92 4 introns intron NOUN dti-1354 92 5 in in ADP dti-1354 92 6 the the DET dti-1354 92 7 bmldh bmldh PROPN dti-1354 92 8 dna dna PROPN dti-1354 92 9 . . PUNCT dti-1354 93 1 alignment alignment NOUN dti-1354 93 2 of of ADP dti-1354 93 3 the the DET dti-1354 93 4 translated translate VERB dti-1354 93 5 bmldh bmldh ADJ dti-1354 93 6 amino amino NOUN dti-1354 93 7 acid acid NOUN dti-1354 93 8 sequence sequence NOUN dti-1354 93 9 with with ADP dti-1354 93 10 that that PRON dti-1354 93 11 of of ADP dti-1354 93 12 related related ADJ dti-1354 93 13 apicomplexan apicomplexan NOUN dti-1354 93 14 parasites parasite NOUN dti-1354 93 15 and and CCONJ dti-1354 93 16 selected select VERB dti-1354 93 17 mammalian mammalian ADJ dti-1354 93 18 ldh ldh PROPN dti-1354 93 19 gave give VERB dti-1354 93 20 contrasting contrasting NOUN dti-1354 93 21 result result NOUN dti-1354 93 22 . . PUNCT dti-1354 94 1 the the DET dti-1354 94 2 amino amino NOUN dti-1354 94 3 acid acid NOUN dti-1354 94 4 sequence sequence NOUN dti-1354 94 5 for for ADP dti-1354 94 6 bmldh bmldh PROPN dti-1354 94 7 was be AUX dti-1354 94 8 less less ADJ dti-1354 94 9 than than ADP dti-1354 94 10 28 28 NUM dti-1354 94 11 % % NOUN dti-1354 94 12 identical identical ADJ dti-1354 94 13 to to ADP dti-1354 94 14 that that PRON dti-1354 94 15 of of ADP dti-1354 94 16 plasmodium plasmodium NOUN dti-1354 94 17 falciparum falciparum NOUN dti-1354 94 18 ( ( PUNCT dti-1354 94 19 accession accession NOUN dti-1354 94 20 no no INTJ dti-1354 94 21 . . PUNCT dti-1354 94 22 aak12097 aak12097 ADJ dti-1354 94 23 ) ) PUNCT dti-1354 94 24 , , PUNCT dti-1354 94 25 t. t. NOUN dti-1354 94 26 gondii gondii NOUN dti-1354 94 27 ( ( PUNCT dti-1354 94 28 accession accession NOUN dti-1354 94 29 no no INTJ dti-1354 94 30 . . PUNCT dti-1354 94 31 xp_002368488 xp_002368488 PROPN dti-1354 94 32 ) ) PUNCT dti-1354 94 33 , , PUNCT dti-1354 94 34 b. b. PROPN dti-1354 94 35 bovis bovis PROPN dti-1354 94 36 ( ( PUNCT dti-1354 94 37 accession accession NOUN dti-1354 94 38 no no INTJ dti-1354 94 39 . . PROPN dti-1354 94 40 edo07479 edo07479 ADV dti-1354 94 41 ) ) PUNCT dti-1354 94 42 , , PUNCT dti-1354 94 43 and and CCONJ dti-1354 94 44 t. t. PROPN dti-1354 94 45 annulata annulata PROPN dti-1354 94 46 ( ( PUNCT dti-1354 94 47 accession accession NOUN dti-1354 94 48 no no INTJ dti-1354 94 49 . . PUNCT dti-1354 94 50 adg45564 adg45564 NUM dti-1354 94 51 ) ) PUNCT dti-1354 94 52 . . PUNCT dti-1354 95 1 however however ADV dti-1354 95 2 , , PUNCT dti-1354 95 3 there there PRON dti-1354 95 4 was be VERB dti-1354 95 5 high high ADJ dti-1354 95 6 frequency frequency NOUN dti-1354 95 7 of of ADP dti-1354 95 8 identical identical ADJ dti-1354 95 9 amino amino NOUN dti-1354 95 10 acid acid NOUN dti-1354 95 11 sequence sequence NOUN dti-1354 95 12 between between ADP dti-1354 95 13 bmldh bmldh ADJ dti-1354 95 14 and and CCONJ dti-1354 95 15 ldh ldh ADJ dti-1354 95 16 mammals mammal NOUN dti-1354 95 17 like like ADP dti-1354 95 18 hamster hamster NOUN dti-1354 95 19 ( ( PUNCT dti-1354 95 20 70.3 70.3 NUM dti-1354 95 21 % % NOUN dti-1354 95 22 , , PUNCT dti-1354 95 23 accession accession NOUN dti-1354 95 24 no no INTJ dti-1354 95 25 . . PUNCT dti-1354 96 1 np_001230979 np_001230979 NOUN dti-1354 96 2 ) ) PUNCT dti-1354 96 3 , , PUNCT dti-1354 97 1 mouse mouse NOUN dti-1354 97 2 ( ( PUNCT dti-1354 97 3 69.1 69.1 NUM dti-1354 97 4 % % NOUN dti-1354 97 5 , , PUNCT dti-1354 97 6 accession accession NOUN dti-1354 97 7 no no PROPN dti-1354 97 8 . . PROPN dti-1354 97 9 caa26360 caa26360 PROPN dti-1354 97 10 ) ) PUNCT dti-1354 97 11 , , PUNCT dti-1354 97 12 and and CCONJ dti-1354 97 13 humans human NOUN dti-1354 97 14 ( ( PUNCT dti-1354 97 15 61.9 61.9 NUM dti-1354 97 16 % % NOUN dti-1354 97 17 , , PUNCT dti-1354 97 18 accession accession NOUN dti-1354 97 19 no no NOUN dti-1354 97 20 . . PUNCT dti-1354 97 21 cae11711 cae11711 PROPN dti-1354 97 22 ) ) PUNCT dti-1354 97 23 . . PUNCT dti-1354 98 1 approximately approximately ADV dti-1354 98 2 43 43 NUM dti-1354 98 3 amino amino NOUN dti-1354 98 4 acid acid NOUN dti-1354 98 5 residues residue NOUN dti-1354 98 6 were be AUX dti-1354 98 7 highly highly ADV dti-1354 98 8 conserved conserved ADJ dti-1354 98 9 in in ADP dti-1354 98 10 all all DET dti-1354 98 11 the the DET dti-1354 98 12 eight eight NUM dti-1354 98 13 ldh ldh NOUN dti-1354 98 14 sequences sequence NOUN dti-1354 98 15 aligned align VERB dti-1354 98 16 ( ( PUNCT dti-1354 98 17 fig fig NOUN dti-1354 98 18 . . NOUN dti-1354 99 1 1 1 NUM dti-1354 99 2 ) ) PUNCT dti-1354 99 3 . . PUNCT dti-1354 100 1 characterization characterization NOUN dti-1354 100 2 of of ADP dti-1354 100 3 expressed express VERB dti-1354 100 4 recombinant recombinant ADJ dti-1354 100 5 bmldh bmldh ADJ dti-1354 100 6 protein protein NOUN dti-1354 100 7 . . PUNCT dti-1354 101 1 the the DET dti-1354 101 2 recombinant recombinant ADJ dti-1354 101 3 gst gst NOUN dti-1354 101 4 - - PUNCT dti-1354 101 5 fussed fuss VERB dti-1354 101 6 bmldh bmldh PROPN dti-1354 101 7 ( ( PUNCT dti-1354 101 8 gstbmldh gstbmldh PROPN dti-1354 101 9 ) ) PUNCT dti-1354 101 10 had have VERB dti-1354 101 11 a a DET dti-1354 101 12 molecular molecular ADJ dti-1354 101 13 weight weight NOUN dti-1354 101 14 of of ADP dti-1354 101 15 approximately approximately ADV dti-1354 101 16 62 62 NUM dti-1354 101 17     SPACE dti-1354 101 18 kda kda NOUN dti-1354 101 19 ( ( PUNCT dti-1354 101 20 fig fig NOUN dti-1354 101 21 . . PUNCT dti-1354 102 1 2b 2b NOUN dti-1354 102 2 , , PUNCT dti-1354 102 3 lane lane NOUN dti-1354 102 4 4 4 NUM dti-1354 102 5 ) ) PUNCT dti-1354 102 6 . . PUNCT dti-1354 103 1 since since SCONJ dti-1354 103 2 the the DET dti-1354 103 3 molecular molecular ADJ dti-1354 103 4 weight weight NOUN dti-1354 103 5 of of ADP dti-1354 103 6 gst gst PROPN dti-1354 103 7 is be AUX dti-1354 103 8 26 26 NUM dti-1354 103 9     SPACE dti-1354 103 10 kda kda NOUN dti-1354 103 11 , , PUNCT dti-1354 103 12 it it PRON dti-1354 103 13 is be AUX dti-1354 103 14 estimated estimate VERB dti-1354 103 15 that that SCONJ dti-1354 103 16 the the DET dti-1354 103 17 molecular molecular ADJ dti-1354 103 18 weight weight NOUN dti-1354 103 19 of of ADP dti-1354 103 20 bmldh bmldh ADJ dti-1354 103 21 protein protein NOUN dti-1354 103 22 is be AUX dti-1354 103 23 36 36 NUM dti-1354 103 24     SPACE dti-1354 103 25 kda kda NOUN dti-1354 103 26 . . PUNCT dti-1354 104 1 over over ADP dti-1354 104 2 70 70 NUM dti-1354 104 3 % % NOUN dti-1354 104 4 of of ADP dti-1354 104 5 the the DET dti-1354 104 6 recombinant recombinant ADJ dti-1354 104 7 protein protein NOUN dti-1354 104 8 was be AUX dti-1354 104 9 insoluble insoluble ADJ dti-1354 104 10 in in ADP dti-1354 104 11 water water NOUN dti-1354 104 12 upon upon SCONJ dti-1354 104 13 over over ADP dti-1354 104 14 expression expression NOUN dti-1354 104 15 in in ADP dti-1354 104 16 e. e. PROPN dti-1354 104 17 coli coli PROPN dti-1354 104 18 ( ( PUNCT dti-1354 104 19 fig fig NOUN dti-1354 104 20 . . PUNCT dti-1354 105 1 2a 2a NUM dti-1354 105 2 , , PUNCT dti-1354 105 3 lane lane NOUN dti-1354 105 4 2 2 NUM dti-1354 105 5 ) ) PUNCT dti-1354 105 6 . . PUNCT dti-1354 106 1 the the DET dti-1354 106 2 average average ADJ dti-1354 106 3 yield yield NOUN dti-1354 106 4 of of ADP dti-1354 106 5 the the DET dti-1354 106 6 gst gst NOUN dti-1354 106 7 - - PUNCT dti-1354 106 8 bmldh bmldh PROPN dti-1354 106 9 and and CCONJ dti-1354 106 10 gst gst NOUN dti-1354 106 11 - - PUNCT dti-1354 106 12 free free ADJ dti-1354 106 13 bmldh bmldh PROPN dti-1354 106 14 from from ADP dti-1354 106 15 the the DET dti-1354 106 16 soluble soluble ADJ dti-1354 106 17 fraction fraction NOUN dti-1354 106 18 was be AUX dti-1354 106 19 approximately approximately ADV dti-1354 106 20 200 200 NUM dti-1354 106 21     SPACE dti-1354 106 22 µg µg VERB dti-1354 106 23 / / SYM dti-1354 106 24 ml ml PROPN dti-1354 106 25 ( ( PUNCT dti-1354 106 26 fig fig NOUN dti-1354 106 27 . . PUNCT dti-1354 106 28 2b 2b NOUN dti-1354 106 29 , , PUNCT dti-1354 106 30 lanes lane VERB dti-1354 106 31 4 4 NUM dti-1354 106 32 and and CCONJ dti-1354 106 33 5 5 NUM dti-1354 106 34 ) ) PUNCT dti-1354 106 35 . . PUNCT dti-1354 107 1 characterization characterization NOUN dti-1354 107 2 of of ADP dti-1354 107 3 native native ADJ dti-1354 107 4 bmldh bmldh ADJ dti-1354 107 5 protein protein NOUN dti-1354 107 6 . . PUNCT dti-1354 108 1 analysis analysis NOUN dti-1354 108 2 of of ADP dti-1354 108 3 parasite parasite NOUN dti-1354 108 4 lysate lysate NOUN dti-1354 108 5 by by ADP dti-1354 108 6 western western ADJ dti-1354 108 7 blot blot NOUN dti-1354 108 8 method method NOUN dti-1354 108 9 revealed reveal VERB dti-1354 108 10 a a DET dti-1354 108 11 specific specific ADJ dti-1354 108 12 36 36 NUM dti-1354 108 13     SPACE dti-1354 108 14 kda kda NOUN dti-1354 108 15 native native PROPN dti-1354 108 16 bmldh bmldh PROPN dti-1354 108 17 ( ( PUNCT dti-1354 108 18 fig fig NOUN dti-1354 108 19 . . PUNCT dti-1354 109 1 3 3 NUM dti-1354 109 2 , , PUNCT dti-1354 109 3 lane lane NOUN dti-1354 109 4 1 1 NUM dti-1354 109 5 ) ) PUNCT dti-1354 109 6 . . PUNCT dti-1354 110 1 no no DET dti-1354 110 2 band band NOUN dti-1354 110 3 appeared appear VERB dti-1354 110 4 when when SCONJ dti-1354 110 5 mice mouse NOUN dti-1354 110 6 spf spf PROPN dti-1354 110 7 sera sera NOUN dti-1354 110 8 was be AUX dti-1354 110 9 used use VERB dti-1354 110 10 as as ADP dti-1354 110 11 control control NOUN dti-1354 110 12 as as SCONJ dti-1354 110 13 shown show VERB dti-1354 110 14 in in ADP dti-1354 110 15 figure figure NOUN dti-1354 110 16 3 3 NUM dti-1354 110 17 , , PUNCT dti-1354 110 18 lane lane NOUN dti-1354 110 19 2 2 NUM dti-1354 110 20 . . PUNCT dti-1354 111 1 the the DET dti-1354 111 2 polyclonal polyclonal ADJ dti-1354 111 3 antibody antibody NOUN dti-1354 111 4 raised raise VERB dti-1354 111 5 against against ADP dti-1354 111 6 recombinant recombinant ADJ dti-1354 111 7 bmldh bmldh PROPN dti-1354 111 8 was be AUX dti-1354 111 9 able able ADJ dti-1354 111 10 to to PART dti-1354 111 11 detect detect VERB dti-1354 111 12 the the DET dti-1354 111 13 native native ADJ dti-1354 111 14 bmldh bmldh PROPN dti-1354 111 15 in in ADP dti-1354 111 16 infected infected ADJ dti-1354 111 17 rbcs rbc NOUN dti-1354 111 18 by by ADP dti-1354 111 19 indirect indirect ADJ dti-1354 111 20 ifat ifat NOUN dti-1354 111 21 . . PUNCT dti-1354 112 1 this this PRON dti-1354 112 2 was be AUX dti-1354 112 3 shown show VERB dti-1354 112 4 by by ADP dti-1354 112 5 specific specific ADJ dti-1354 112 6 green green ADJ dti-1354 112 7 fluorescence fluorescence NOUN dti-1354 112 8 observed observe VERB dti-1354 112 9 in in ADP dti-1354 112 10 the the DET dti-1354 112 11 cytoplasm cytoplasm NOUN dti-1354 112 12 and and CCONJ dti-1354 112 13 nucleus nucleus NOUN dti-1354 112 14 of of ADP dti-1354 112 15 b. b. PROPN dti-1354 112 16 microti microti PROPN dti-1354 112 17 merozoites merozoites PROPN dti-1354 112 18 ( ( PUNCT dti-1354 112 19 fig fig NOUN dti-1354 112 20 . . PUNCT dti-1354 112 21 4 4 NUM dti-1354 112 22 ) ) PUNCT dti-1354 112 23 . . PUNCT dti-1354 113 1 the the DET dti-1354 113 2 location location NOUN dti-1354 113 3 of of ADP dti-1354 113 4 the the DET dti-1354 113 5 green green ADJ dti-1354 113 6 fluorescence fluorescence NOUN dti-1354 113 7 in in ADP dti-1354 113 8 both both CCONJ dti-1354 113 9 the the DET dti-1354 113 10 merozoite merozoite ADJ dti-1354 113 11 cytoplasm cytoplasm NOUN dti-1354 113 12 and and CCONJ dti-1354 113 13 nucleus nucleus NOUN dti-1354 113 14 is be AUX dti-1354 113 15 consistent consistent ADJ dti-1354 113 16 with with ADP dti-1354 113 17 the the DET dti-1354 113 18 finding finding NOUN dti-1354 113 19 from from ADP dti-1354 113 20 the the DET dti-1354 113 21 cnls cnls PROPN dti-1354 113 22 mapper mapper PROPN dti-1354 113 23 , , PUNCT dti-1354 113 24 which which PRON dti-1354 113 25 showed show VERB dti-1354 113 26 that that SCONJ dti-1354 113 27 bmldh bmldh PROPN dti-1354 113 28 has have VERB dti-1354 113 29 both both CCONJ dti-1354 113 30 cytoplasmic cytoplasmic ADJ dti-1354 113 31 and and CCONJ dti-1354 113 32 nucleus nucleus ADJ dti-1354 113 33 localization localization NOUN dti-1354 113 34 signal signal VERB dti-1354 113 35 sequence sequence NOUN dti-1354 113 36 from from ADP dti-1354 113 37 amino amino NOUN dti-1354 113 38 acid acid NOUN dti-1354 113 39 number number NOUN dti-1354 113 40 241–269 241–269 NUM dti-1354 113 41 ( ( PUNCT dti-1354 113 42 eiiklkgytswaiglsvgdlscsliknlr eiiklkgytswaiglsvgdlscsliknlr PROPN dti-1354 113 43 ) ) PUNCT dti-1354 113 44 . . PUNCT dti-1354 114 1 bmldh bmldh ADJ dti-1354 114 2 enzyme enzyme NOUN dti-1354 114 3 activity activity NOUN dti-1354 114 4 . . PUNCT dti-1354 115 1 optimal optimal ADJ dti-1354 115 2 ph ph PROPN dti-1354 115 3 , , PUNCT dti-1354 115 4 vmax vmax PROPN dti-1354 115 5 , , PUNCT dti-1354 115 6 and and CCONJ dti-1354 115 7 km km NOUN dti-1354 115 8 . . PUNCT dti-1354 116 1 the the DET dti-1354 116 2 recombinant recombinant ADJ dti-1354 116 3 bmldh bmldh ADJ dti-1354 116 4 protein protein NOUN dti-1354 116 5 is be AUX dti-1354 116 6 an an DET dti-1354 116 7 active active ADJ dti-1354 116 8 enzyme enzyme NOUN dti-1354 116 9 with with ADP dti-1354 116 10 a a DET dti-1354 116 11 high high ADJ dti-1354 116 12 catalytic catalytic ADJ dti-1354 116 13 efficiency efficiency NOUN dti-1354 116 14 at at ADP dti-1354 116 15 optimal optimal ADJ dti-1354 116 16 ph ph NOUN dti-1354 116 17 of of ADP dti-1354 116 18 10.2 10.2 NUM dti-1354 116 19 ( ( PUNCT dti-1354 116 20 fig fig NOUN dti-1354 116 21 . . PUNCT dti-1354 117 1 5a 5a NUM dti-1354 117 2 ) ) PUNCT dti-1354 117 3 . . PUNCT dti-1354 118 1 the the DET dti-1354 118 2 km km NOUN dti-1354 118 3 values value NOUN dti-1354 118 4 for for ADP dti-1354 118 5 nad+ nad+ PROPN dti-1354 118 6 and and CCONJ dti-1354 118 7 lactate lactate PROPN dti-1354 118 8 were be AUX dti-1354 118 9 8.7 8.7 NUM dti-1354 118 10     SPACE dti-1354 118 11 ± ± NOUN dti-1354 118 12     SPACE dti-1354 118 13 0.57 0.57 NUM dti-1354 118 14     SPACE dti-1354 118 15 mm mm PROPN dti-1354 118 16 and and CCONJ dti-1354 118 17 99.9 99.9 NUM dti-1354 118 18     SPACE dti-1354 118 19 ± ± NOUN dti-1354 118 20     SPACE dti-1354 118 21 22.33 22.33 NUM dti-1354 118 22     SPACE dti-1354 118 23 mm mm NOUN dti-1354 118 24 , , PUNCT dti-1354 118 25 respectively respectively ADV dti-1354 118 26 ( ( PUNCT dti-1354 118 27 fig fig NOUN dti-1354 118 28 . . PUNCT dti-1354 119 1 5b 5b NUM dti-1354 119 2 and and CCONJ dti-1354 119 3 c c NOUN dti-1354 119 4 ) ) PUNCT dti-1354 119 5 . . PUNCT dti-1354 120 1 bmldh bmldh PROPN dti-1354 120 2 had have VERB dti-1354 120 3 very very ADV dti-1354 120 4 high high ADJ dti-1354 120 5 catalytic catalytic ADJ dti-1354 120 6 activity activity NOUN dti-1354 120 7 for for ADP dti-1354 120 8 both both DET dti-1354 120 9 nad+ nad+ PROPN dti-1354 120 10 ( ( PUNCT dti-1354 120 11 6.3 6.3 NUM dti-1354 120 12     SPACE dti-1354 120 13 × × NOUN dti-1354 120 14     SPACE dti-1354 120 15 105 105 NUM dti-1354 120 16 minute-1 minute-1 NUM dti-1354 120 17 ) ) PUNCT dti-1354 120 18 and and CCONJ dti-1354 120 19 lactate lactate NOUN dti-1354 120 20 ( ( PUNCT dti-1354 120 21 7.3 7.3 NUM dti-1354 120 22     SPACE dti-1354 120 23 × × NOUN dti-1354 120 24     SPACE dti-1354 120 25 105 105 NUM dti-1354 120 26 minute-1 minute-1 NUM dti-1354 120 27 ) ) PUNCT dti-1354 120 28 . . PUNCT dti-1354 121 1 similarly similarly ADV dti-1354 121 2 , , PUNCT dti-1354 121 3 a a DET dti-1354 121 4 catalytic catalytic ADJ dti-1354 121 5 efficiency efficiency NOUN dti-1354 121 6 of of ADP dti-1354 121 7 5.126 5.126 NUM dti-1354 121 8     SPACE dti-1354 121 9 × × NOUN dti-1354 121 10     SPACE dti-1354 121 11 108 108 NUM dti-1354 121 12     SPACE dti-1354 121 13 m-1 m-1 NOUN dti-1354 121 14     SPACE dti-1354 121 15 minute-1 minute-1 PROPN dti-1354 121 16 and and CCONJ dti-1354 121 17 7.3003 7.3003 NUM dti-1354 121 18     SPACE dti-1354 121 19 × × NOUN dti-1354 121 20     SPACE dti-1354 121 21 106 106 NUM dti-1354 121 22     SPACE dti-1354 121 23 m-1 m-1 NUM dti-1354 121 24     SPACE dti-1354 121 25 minute-1 minute-1 NOUN dti-1354 121 26 was be AUX dti-1354 121 27 recorded record VERB dti-1354 121 28 for for ADP dti-1354 121 29 nad+ nad+ PROPN dti-1354 121 30 and and CCONJ dti-1354 121 31 lactate lactate PROPN dti-1354 121 32 , , PUNCT dti-1354 121 33 respectively respectively ADV dti-1354 121 34 ( ( PUNCT dti-1354 121 35 table table NOUN dti-1354 121 36 1 1 NUM dti-1354 121 37 ) ) PUNCT dti-1354 121 38 . . PUNCT dti-1354 122 1 inhibition inhibition NOUN dti-1354 122 2 of of ADP dti-1354 122 3 bmldh bmldh ADJ dti-1354 122 4 by by ADP dti-1354 122 5 gossypol gossypol PROPN dti-1354 122 6 . . PUNCT dti-1354 123 1 gossypol gossypol PROPN dti-1354 123 2 ( ( PUNCT dti-1354 123 3 nad+ nad+ PROPN dti-1354 123 4 analog analog NOUN dti-1354 123 5 ) ) PUNCT dti-1354 123 6 caused cause VERB dti-1354 123 7 over over ADP dti-1354 123 8 20 20 NUM dti-1354 123 9 % % NOUN dti-1354 123 10 inhibition inhibition NOUN dti-1354 123 11 of of ADP dti-1354 123 12 bmldh bmldh ADJ dti-1354 123 13 catalytic catalytic ADJ dti-1354 123 14 activity activity NOUN dti-1354 123 15 at at ADP dti-1354 123 16 very very ADV dti-1354 123 17 low low ADJ dti-1354 123 18 concentration concentration NOUN dti-1354 123 19 of of ADP dti-1354 123 20 9.5 9.5 NUM dti-1354 123 21     SPACE dti-1354 123 22 nm nm NOUN dti-1354 123 23 . . PUNCT dti-1354 124 1 moreover moreover ADV dti-1354 124 2 , , PUNCT dti-1354 124 3 100 100 NUM dti-1354 124 4 % % NOUN dti-1354 124 5 inhibition inhibition NOUN dti-1354 124 6 of of ADP dti-1354 124 7 bmldh bmldh ADJ dti-1354 124 8 enzyme enzyme NOUN dti-1354 124 9 activity activity NOUN dti-1354 124 10 was be AUX dti-1354 124 11 recorded record VERB dti-1354 124 12 when when SCONJ dti-1354 124 13 2.5 2.5 NUM dti-1354 124 14     SPACE dti-1354 124 15 µm µm NOUN dti-1354 124 16 http://www.la-press.com http://www.la-press.com X dti-1354 124 17 vudriko vudriko ADV dti-1354 124 18 et et PROPN dti-1354 124 19 al al PROPN dti-1354 124 20 34 34 NUM dti-1354 124 21 drug drug NOUN dti-1354 124 22 target target NOUN dti-1354 124 23 insights insight NOUN dti-1354 124 24 2014:8 2014:8 NUM dti-1354 124 25 figure figure NOUN dti-1354 124 26 1 1 NUM dti-1354 124 27 . . PUNCT dti-1354 124 28 multiple multiple ADJ dti-1354 124 29 amino amino NOUN dti-1354 124 30 acid acid NOUN dti-1354 124 31 sequence sequence NOUN dti-1354 124 32 alignment alignment NOUN dti-1354 124 33 and and CCONJ dti-1354 124 34 identity identity NOUN dti-1354 124 35 score score NOUN dti-1354 124 36 between between ADP dti-1354 124 37 bmldh bmldh PROPN dti-1354 124 38 and and CCONJ dti-1354 124 39 ldh ldh NOUN dti-1354 124 40 for for ADP dti-1354 124 41 p. p. NOUN dti-1354 124 42 falciparum falciparum PROPN dti-1354 124 43 ( ( PUNCT dti-1354 124 44 pfldh pfldh NOUN dti-1354 124 45 ) ) PUNCT dti-1354 124 46 , , PUNCT dti-1354 124 47 b. b. PROPN dti-1354 124 48 bovis bovis PROPN dti-1354 124 49 ( ( PUNCT dti-1354 124 50 bbldh bbldh ADJ dti-1354 124 51 ) ) PUNCT dti-1354 124 52 , , PUNCT dti-1354 124 53 t. t. NOUN dti-1354 124 54 gondii gondii PROPN dti-1354 124 55 ( ( PUNCT dti-1354 124 56 tgldh tgldh NOUN dti-1354 124 57 ) ) PUNCT dti-1354 124 58 , , PUNCT dti-1354 124 59 t. t. PROPN dti-1354 124 60 annulata annulata PROPN dti-1354 124 61 ( ( PUNCT dti-1354 124 62 taldh taldh NOUN dti-1354 124 63 ) ) PUNCT dti-1354 124 64 , , PUNCT dti-1354 124 65 mus mus PROPN dti-1354 124 66 musculus musculus PROPN dti-1354 124 67 ( ( PUNCT dti-1354 124 68 mouse mouse PROPN dti-1354 124 69 ldh ldh PROPN dti-1354 124 70 ) ) PUNCT dti-1354 124 71 , , PUNCT dti-1354 124 72 cricetulus cricetulus PROPN dti-1354 124 73 griseus griseus PROPN dti-1354 124 74 ( ( PUNCT dti-1354 124 75 hamster hamster PROPN dti-1354 124 76 ldh ldh PROPN dti-1354 124 77 ) ) PUNCT dti-1354 124 78 , , PUNCT dti-1354 124 79 and and CCONJ dti-1354 124 80 human human PROPN dti-1354 124 81 ( ( PUNCT dti-1354 124 82 h. h. PROPN dti-1354 124 83 sapiens sapiens PROPN dti-1354 124 84 ldh ldh PROPN dti-1354 124 85 ) ) PUNCT dti-1354 124 86 . . PUNCT dti-1354 125 1 m2 m2 PROPN dti-1354 125 2 4 4 NUM dti-1354 125 3 97 97 NUM dti-1354 125 4 66 66 NUM dti-1354 125 5 45 45 NUM dti-1354 125 6 kd kd PROPN dti-1354 125 7 a a DET dti-1354 125 8 kd kd PROPN dti-1354 125 9 a a DET dti-1354 125 10 30 30 NUM dti-1354 125 11 14 14 NUM dti-1354 125 12 5 5 NUM dti-1354 125 13 62 62 NUM dti-1354 125 14 kda kda NOUN dti-1354 125 15 36 36 NUM dti-1354 125 16 kda kda NOUN dti-1354 125 17 2 2 NUM dti-1354 125 18 3 3 NUM dti-1354 125 19 50 50 NUM dti-1354 125 20 37 37 NUM dti-1354 125 21 25 25 NUM dti-1354 125 22 20 20 NUM dti-1354 125 23 15 15 NUM dti-1354 125 24 250 250 NUM dti-1354 125 25 m1 m1 PROPN dti-1354 125 26 a a DET dti-1354 125 27 b b NOUN dti-1354 125 28 1 1 NUM dti-1354 125 29 75 75 NUM dti-1354 125 30 figure figure NOUN dti-1354 125 31 2 2 NUM dti-1354 125 32 . . X dti-1354 125 33 sds sds NOUN dti-1354 125 34 - - PUNCT dti-1354 125 35 page page NOUN dti-1354 125 36 ( ( PUNCT dti-1354 125 37 a a X dti-1354 125 38 ) ) PUNCT dti-1354 125 39 showing show VERB dti-1354 125 40 non non ADJ dti-1354 125 41 - - ADJ dti-1354 125 42 induced induced ADJ dti-1354 125 43 control control NOUN dti-1354 125 44 ( ( PUNCT dti-1354 125 45 lane lane NOUN dti-1354 125 46 1 1 NUM dti-1354 125 47 ) ) PUNCT dti-1354 125 48 , , PUNCT dti-1354 125 49 expressed express VERB dti-1354 125 50 protein protein NOUN dti-1354 125 51 in in ADP dti-1354 125 52 pellet pellet NOUN dti-1354 125 53 ( ( PUNCT dti-1354 125 54 lane lane NOUN dti-1354 125 55 2 2 NUM dti-1354 125 56 ) ) PUNCT dti-1354 125 57 , , PUNCT dti-1354 125 58 and and CCONJ dti-1354 125 59 supernatant supernatant NOUN dti-1354 125 60 / / SYM dti-1354 125 61 soluble soluble ADJ dti-1354 125 62 fraction fraction NOUN dti-1354 125 63 ( ( PUNCT dti-1354 125 64 lanes lane NOUN dti-1354 125 65 3 3 NUM dti-1354 125 66 ) ) PUNCT dti-1354 125 67 ; ; PUNCT dti-1354 125 68 sdspage sdspage NOUN dti-1354 125 69 ( ( PUNCT dti-1354 125 70 b b NOUN dti-1354 125 71 ) ) PUNCT dti-1354 125 72 for for ADP dti-1354 125 73 purified purified ADJ dti-1354 125 74 gst gst NOUN dti-1354 125 75 - - PUNCT dti-1354 125 76 bmldh bmldh ADJ dti-1354 125 77 protein protein NOUN dti-1354 125 78 ( ( PUNCT dti-1354 125 79 lanes lane NOUN dti-1354 125 80 4 4 NUM dti-1354 125 81 ) ) PUNCT dti-1354 125 82 and and CCONJ dti-1354 125 83 prescission prescission NOUN dti-1354 125 84 ™ ™ ADJ dti-1354 125 85 protease protease NOUN dti-1354 125 86 cleaved cleave VERB dti-1354 125 87 bmldh bmldh ADJ dti-1354 125 88 ( ( PUNCT dti-1354 125 89 lane lane NOUN dti-1354 125 90 5 5 NUM dti-1354 125 91 ) ) PUNCT dti-1354 125 92 . . PUNCT dti-1354 126 1 http://www.la-press.com http://www.la-press.com X dti-1354 127 1 molecular molecular ADJ dti-1354 127 2 and and CCONJ dti-1354 127 3 kinetic kinetic ADJ dti-1354 127 4 characterization characterization NOUN dti-1354 127 5 of of ADP dti-1354 127 6 b. b. PROPN dti-1354 127 7 microti microti PROPN dti-1354 127 8 ldh ldh PROPN dti-1354 127 9 35drug 35drug PROPN dti-1354 127 10 target target NOUN dti-1354 127 11 insights insight NOUN dti-1354 127 12 2014:8 2014:8 NUM dti-1354 127 13 1 1 NUM dti-1354 127 14 a a DET dti-1354 127 15 b b NOUN dti-1354 127 16 2 2 NUM dti-1354 127 17 3 3 NUM dti-1354 127 18 figure figure NOUN dti-1354 127 19 4 4 NUM dti-1354 127 20 . . PUNCT dti-1354 128 1 ( ( PUNCT dti-1354 128 2 a a X dti-1354 128 3 ) ) PUNCT dti-1354 128 4 cytoplasmic cytoplasmic ADJ dti-1354 128 5 localization localization NOUN dti-1354 128 6 of of ADP dti-1354 128 7 bmldh bmldh PROPN dti-1354 128 8 in in ADP dti-1354 128 9 merozoite merozoite NOUN dti-1354 128 10 ; ; PUNCT dti-1354 128 11 ( ( PUNCT dti-1354 128 12 b b X dti-1354 128 13 ) ) PUNCT dti-1354 128 14 nucleus nucleus ADJ dti-1354 128 15 localization localization NOUN dti-1354 128 16 of of ADP dti-1354 128 17 bmldh bmldh ADJ dti-1354 128 18 ; ; PUNCT dti-1354 128 19 ( ( PUNCT dti-1354 128 20 1 1 X dti-1354 128 21 ) ) PUNCT dti-1354 128 22 immunoflourescent immunoflourescent NOUN dti-1354 128 23 staining stain VERB dti-1354 128 24 of of ADP dti-1354 128 25 b. b. PROPN dti-1354 128 26 microti microti PROPN dti-1354 128 27 with with ADP dti-1354 128 28 polyclonal polyclonal ADJ dti-1354 128 29 bmldh bmldh ADJ dti-1354 128 30 and and CCONJ dti-1354 128 31 anti anti ADJ dti-1354 128 32 - - ADJ dti-1354 128 33 mouse mouse ADJ dti-1354 128 34 alexa-488 alexa-488 NOUN dti-1354 128 35 ; ; PUNCT dti-1354 128 36 ( ( PUNCT dti-1354 128 37 2 2 X dti-1354 128 38 ) ) PUNCT dti-1354 128 39 b. b. PROPN dti-1354 128 40 microti microti PROPN dti-1354 128 41 merozoite merozoite PROPN dti-1354 128 42 nuclei nucleus NOUN dti-1354 128 43 stained stain VERB dti-1354 128 44 with with ADP dti-1354 128 45 pi pi NOUN dti-1354 128 46 ; ; PUNCT dti-1354 128 47 and and CCONJ dti-1354 128 48 ( ( PUNCT dti-1354 128 49 3 3 X dti-1354 128 50 ) ) PUNCT dti-1354 128 51 merged merge VERB dti-1354 128 52 image image NOUN dti-1354 128 53 ; ; PUNCT dti-1354 128 54 scale scale NOUN dti-1354 128 55 bar bar NOUN dti-1354 128 56 is be AUX dti-1354 128 57 5 5 NUM dti-1354 128 58 and and CCONJ dti-1354 128 59 2.5 2.5 NUM dti-1354 128 60 µm µm NOUN dti-1354 128 61 for for ADP dti-1354 128 62 a a PRON dti-1354 128 63 and and CCONJ dti-1354 128 64 b b NOUN dti-1354 128 65 , , PUNCT dti-1354 128 66 respectively respectively ADV dti-1354 128 67 . . PUNCT dti-1354 129 1 kda kda PROPN dti-1354 129 2 m m PROPN dti-1354 129 3 1 1 NUM dti-1354 129 4 2 2 NUM dti-1354 129 5 97 97 NUM dti-1354 129 6 66 66 NUM dti-1354 129 7 45 45 NUM dti-1354 129 8 36 36 NUM dti-1354 129 9 kda kda NOUN dti-1354 129 10 30 30 NUM dti-1354 129 11 14 14 NUM dti-1354 129 12 figure figure NOUN dti-1354 129 13 3 3 NUM dti-1354 129 14 . . PUNCT dti-1354 129 15 western western ADJ dti-1354 129 16 blot blot NOUN dti-1354 129 17 lane lane PROPN dti-1354 129 18 m m NOUN dti-1354 129 19 : : PUNCT dti-1354 129 20 molecular molecular ADJ dti-1354 129 21 marker marker NOUN dti-1354 129 22 stained stain VERB dti-1354 129 23 with with ADP dti-1354 129 24 amide amide NOUN dti-1354 129 25 black black NOUN dti-1354 129 26 ; ; PUNCT dti-1354 129 27 lane lane NOUN dti-1354 129 28 1 1 NUM dti-1354 129 29 is be AUX dti-1354 129 30 36 36 NUM dti-1354 129 31 kda kda NOUN dti-1354 129 32 native native ADJ dti-1354 129 33 bmldh bmldh PROPN dti-1354 129 34 in in ADP dti-1354 129 35 parasite parasite NOUN dti-1354 129 36 lysate lysate NOUN dti-1354 129 37 after after ADP dti-1354 129 38 probe probe NOUN dti-1354 129 39 with with ADP dti-1354 129 40 polyclonal polyclonal ADJ dti-1354 129 41 sera sera NOUN dti-1354 129 42 raised raise VERB dti-1354 129 43 against against ADP dti-1354 129 44 bmldh bmldh ADJ dti-1354 129 45 ; ; PUNCT dti-1354 129 46 lane lane NOUN dti-1354 129 47 2 2 NUM dti-1354 129 48 is be AUX dti-1354 129 49 parasite parasite ADJ dti-1354 129 50 lysate lysate NOUN dti-1354 129 51 probed probe VERB dti-1354 129 52 with with ADP dti-1354 129 53 spf spf ADJ dti-1354 129 54 sera sera NOUN dti-1354 129 55 ( ( PUNCT dti-1354 129 56 negative negative ADJ dti-1354 129 57 control control NOUN dti-1354 129 58 ) ) PUNCT dti-1354 129 59 . . PUNCT dti-1354 130 1 of of ADP dti-1354 130 2 the the DET dti-1354 130 3 inhibitor inhibitor NOUN dti-1354 130 4 was be AUX dti-1354 130 5 added add VERB dti-1354 130 6 to to ADP dti-1354 130 7 the the DET dti-1354 130 8 assay assay NOUN dti-1354 130 9 . . PUNCT dti-1354 131 1 the the DET dti-1354 131 2 ic50 ic50 PROPN dti-1354 131 3 value value NOUN dti-1354 131 4 for for ADP dti-1354 131 5 gossypol gossypol PROPN dti-1354 131 6 was be AUX dti-1354 131 7 345.0 345.0 NUM dti-1354 131 8     SPACE dti-1354 131 9 ± ± NOUN dti-1354 131 10     SPACE dti-1354 131 11 1.11 1.11 NUM dti-1354 131 12     SPACE dti-1354 131 13 nm nm NOUN dti-1354 131 14 ( ( PUNCT dti-1354 131 15 fig fig NOUN dti-1354 131 16 . . PUNCT dti-1354 132 1 5d 5d NUM dti-1354 132 2 ) ) PUNCT dti-1354 132 3 . . PUNCT dti-1354 133 1 discussion discussion NOUN dti-1354 133 2 this this DET dti-1354 133 3 study study NOUN dti-1354 133 4 revealed reveal VERB dti-1354 133 5 that that SCONJ dti-1354 133 6 both both DET dti-1354 133 7 b. b. PROPN dti-1354 133 8 microti microti PROPN dti-1354 133 9 gray gray ADJ dti-1354 133 10 strain strain NOUN dti-1354 133 11 and and CCONJ dti-1354 133 12 r1 r1 NOUN dti-1354 133 13 strain strain NOUN dti-1354 133 14 have have VERB dti-1354 133 15 identical identical ADJ dti-1354 133 16 ldh ldh NOUN dti-1354 133 17 and and CCONJ dti-1354 133 18 share share VERB dti-1354 133 19 high high ADJ dti-1354 133 20 frequency frequency NOUN dti-1354 133 21 of of ADP dti-1354 133 22 identical identical ADJ dti-1354 133 23 amino amino NOUN dti-1354 133 24 acid acid NOUN dti-1354 133 25 with with ADP dti-1354 133 26 their their PRON dti-1354 133 27 mammalian mammalian ADJ dti-1354 133 28 counterparts counterpart NOUN dti-1354 133 29 . . PUNCT dti-1354 134 1 this this PRON dti-1354 134 2 suggests suggest VERB dti-1354 134 3 that that SCONJ dti-1354 134 4 b. b. PROPN dti-1354 134 5 microti microti PROPN dti-1354 134 6 ldh ldh PROPN dti-1354 134 7 was be AUX dti-1354 134 8 probably probably ADV dti-1354 134 9 acquired acquire VERB dti-1354 134 10 through through ADP dti-1354 134 11 lateral lateral ADJ dti-1354 134 12 gene gene NOUN dti-1354 134 13 transfer transfer NOUN dti-1354 134 14 . . PUNCT dti-1354 135 1 this this PRON dti-1354 135 2 is be AUX dti-1354 135 3 possible possible ADJ dti-1354 135 4 because because SCONJ dti-1354 135 5 the the DET dti-1354 135 6 chromosome chromosome NOUN dti-1354 135 7 on on ADP dti-1354 135 8 which which PRON dti-1354 135 9 the the DET dti-1354 135 10 ldh ldh PROPN dti-1354 135 11 gene gene NOUN dti-1354 135 12 is be AUX dti-1354 135 13 located locate VERB dti-1354 135 14 is be AUX dti-1354 135 15 known know VERB dti-1354 135 16 to to PART dti-1354 135 17 be be AUX dti-1354 135 18 susceptible susceptible ADJ dti-1354 135 19 to to ADP dti-1354 135 20 double double ADJ dti-1354 135 21 - - PUNCT dti-1354 135 22 stranded strand VERB dti-1354 135 23 breaks break NOUN dti-1354 135 24 , , PUNCT dti-1354 135 25 hence hence ADV dti-1354 135 26 suitable suitable ADJ dti-1354 135 27 for for SCONJ dti-1354 135 28 insertion insertion NOUN dti-1354 135 29 of of ADP dti-1354 135 30 foreign foreign ADJ dti-1354 135 31 dna.14 dna.14 PRON dti-1354 135 32 the the DET dti-1354 135 33 acquired acquire VERB dti-1354 135 34 genetic genetic ADJ dti-1354 135 35 sequence sequence NOUN dti-1354 135 36 can can AUX dti-1354 135 37 often often ADV dti-1354 135 38 be be AUX dti-1354 135 39 modified modify VERB dti-1354 135 40 by by ADP dti-1354 135 41 the the DET dti-1354 135 42 parasite parasite NOUN dti-1354 135 43 to to PART dti-1354 135 44 suit suit VERB dti-1354 135 45 its its PRON dti-1354 135 46 survival survival NOUN dti-1354 135 47 mechanism mechanism NOUN dti-1354 135 48 in in ADP dti-1354 135 49 the the DET dti-1354 135 50 host.25 host.25 NOUN dti-1354 135 51 however however ADV dti-1354 135 52 , , PUNCT dti-1354 135 53 it it PRON dti-1354 135 54 is be AUX dti-1354 135 55 worth worth ADJ dti-1354 135 56 noting note VERB dti-1354 135 57 that that SCONJ dti-1354 135 58 despite despite SCONJ dti-1354 135 59 the the DET dti-1354 135 60 above above ADJ dti-1354 135 61 similarity similarity NOUN dti-1354 135 62 in in ADP dti-1354 135 63 amino amino NOUN dti-1354 135 64 acid acid NOUN dti-1354 135 65 sequence sequence NOUN dti-1354 135 66 , , PUNCT dti-1354 135 67 bmldh bmldh PROPN dti-1354 135 68 has have VERB dti-1354 135 69 different different ADJ dti-1354 135 70 amino amino NOUN dti-1354 135 71 acid acid NOUN dti-1354 135 72 residues residue NOUN dti-1354 135 73 in in ADP dti-1354 135 74 its its PRON dti-1354 135 75 antigenic antigenic ADJ dti-1354 135 76 domains domain NOUN dti-1354 135 77 ( ( PUNCT dti-1354 135 78 supplementary supplementary ADJ dti-1354 135 79 table table NOUN dti-1354 135 80 1 1 NUM dti-1354 135 81 ) ) PUNCT dti-1354 135 82 . . PUNCT dti-1354 136 1 this this PRON dti-1354 136 2 suggests suggest VERB dti-1354 136 3 that that SCONJ dti-1354 136 4 the the DET dti-1354 136 5 host host NOUN dti-1354 136 6 immune immune ADJ dti-1354 136 7 system system NOUN dti-1354 136 8 can can AUX dti-1354 136 9 detect detect VERB dti-1354 136 10 the the DET dti-1354 136 11 antigenic antigenic ADJ dti-1354 136 12 epitopes epitope NOUN dti-1354 136 13 of of ADP dti-1354 136 14 bmldh bmldh ADJ dti-1354 136 15 and and CCONJ dti-1354 136 16 produce produce VERB dti-1354 136 17 antibodies antibody NOUN dti-1354 136 18 against against ADP dti-1354 136 19 them they PRON dti-1354 136 20 . . PUNCT dti-1354 137 1 thus thus ADV dti-1354 137 2 , , PUNCT dti-1354 137 3 explaining explain VERB dti-1354 137 4 why why SCONJ dti-1354 137 5 anti anti ADJ dti-1354 137 6 - - ADJ dti-1354 137 7 rbmldh rbmldh ADJ dti-1354 137 8 polyclonal polyclonal ADJ dti-1354 137 9 sera sera NOUN dti-1354 137 10 raised raise VERB dti-1354 137 11 in in ADP dti-1354 137 12 mice mouse NOUN dti-1354 137 13 was be AUX dti-1354 137 14 able able ADJ dti-1354 137 15 to to PART dti-1354 137 16 detect detect VERB dti-1354 137 17 native native ADJ dti-1354 137 18 bmldh bmldh PROPN dti-1354 137 19 by by ADP dti-1354 137 20 western western ADJ dti-1354 137 21 blot blot NOUN dti-1354 137 22 and and CCONJ dti-1354 137 23 ifat ifat NOUN dti-1354 137 24 . . PUNCT dti-1354 138 1 confocal confocal ADJ dti-1354 138 2 laser laser NOUN dti-1354 138 3 microscopy microscopy NOUN dti-1354 138 4 revealed reveal VERB dti-1354 138 5 that that SCONJ dti-1354 138 6 native native ADJ dti-1354 138 7 bmldh bmldh PROPN dti-1354 138 8 is be AUX dti-1354 138 9 dominantly dominantly ADV dti-1354 138 10 localized localize VERB dti-1354 138 11 in in ADP dti-1354 138 12 the the DET dti-1354 138 13 cytoplasma cytoplasma NOUN dti-1354 138 14 , , PUNCT dti-1354 138 15 although although SCONJ dti-1354 138 16 cases case NOUN dti-1354 138 17 of of ADP dti-1354 138 18 nuclear nuclear ADJ dti-1354 138 19 localization localization NOUN dti-1354 138 20 were be AUX dti-1354 138 21 also also ADV dti-1354 138 22 observed observe VERB dti-1354 138 23 . . PUNCT dti-1354 139 1 the the DET dti-1354 139 2 above above ADJ dti-1354 139 3 finding finding NOUN dti-1354 139 4 agrees agree VERB dti-1354 139 5 with with ADP dti-1354 139 6 previous previous ADJ dti-1354 139 7 reports report NOUN dti-1354 139 8 that that SCONJ dti-1354 139 9 while while SCONJ dti-1354 139 10 ldh ldh NOUN dti-1354 139 11 is be AUX dti-1354 139 12 dominantly dominantly ADV dti-1354 139 13 expressed express VERB dti-1354 139 14 in in ADP dti-1354 139 15 the the DET dti-1354 139 16 cytoplasm cytoplasm NOUN dti-1354 139 17 , , PUNCT dti-1354 139 18 it it PRON dti-1354 139 19 can can AUX dti-1354 139 20 be be AUX dti-1354 139 21 found find VERB dti-1354 139 22 in in ADP dti-1354 139 23 the the DET dti-1354 139 24 nucleus.26,27 nucleus.26,27 NOUN dti-1354 139 25 the the DET dti-1354 139 26 role role NOUN dti-1354 139 27 of of ADP dti-1354 139 28 ldh ldh NOUN dti-1354 139 29 in in ADP dti-1354 139 30 the the DET dti-1354 139 31 nucleus nucleus NOUN dti-1354 139 32 remains remain VERB dti-1354 139 33 unclear unclear ADJ dti-1354 140 1 but but CCONJ dti-1354 140 2 it it PRON dti-1354 140 3 is be AUX dti-1354 140 4 understood understand VERB dti-1354 140 5 that that SCONJ dti-1354 140 6 their their PRON dti-1354 140 7 association association NOUN dti-1354 140 8 with with ADP dti-1354 140 9 dna dna PROPN dti-1354 140 10 may may AUX dti-1354 140 11 play play VERB dti-1354 140 12 a a DET dti-1354 140 13 role role NOUN dti-1354 140 14 in in ADP dti-1354 140 15 transcription transcription NOUN dti-1354 140 16 and and CCONJ dti-1354 140 17 replication replication NOUN dti-1354 140 18 of of ADP dti-1354 140 19 dna dna PROPN dti-1354 140 20 through through ADP dti-1354 140 21 general general ADJ dti-1354 140 22 stabilization stabilization NOUN dti-1354 140 23 of of ADP dti-1354 140 24 the the DET dti-1354 140 25 nuclear nuclear ADJ dti-1354 140 26 matrix matrix NOUN dti-1354 140 27 or or CCONJ dti-1354 140 28 chromatin chromatin NOUN dti-1354 140 29 structure.27 structure.27 X dti-1354 140 30 the the DET dti-1354 140 31 enzyme enzyme NOUN dti-1354 140 32 kinetic kinetic ADJ dti-1354 140 33 studies study NOUN dti-1354 140 34 revealed reveal VERB dti-1354 140 35 that that SCONJ dti-1354 140 36 bmldh bmldh PROPN dti-1354 140 37 has have VERB dti-1354 140 38 higher high ADJ dti-1354 140 39 catalytic catalytic ADJ dti-1354 140 40 activity activity NOUN dti-1354 140 41 and and CCONJ dti-1354 140 42 efficiency efficiency NOUN dti-1354 140 43 . . PUNCT dti-1354 141 1 this this DET dti-1354 141 2 finding finding NOUN dti-1354 141 3 is be AUX dti-1354 141 4 consistent consistent ADJ dti-1354 141 5 with with ADP dti-1354 141 6 earlier early ADJ dti-1354 141 7 report report NOUN dti-1354 141 8 that that SCONJ dti-1354 141 9 b. b. PROPN dti-1354 141 10 microti microti PROPN dti-1354 141 11 depends depend VERB dti-1354 141 12 extensively extensively ADV dti-1354 141 13 on on ADP dti-1354 141 14 glycolysis glycolysis NOUN dti-1354 141 15 for for ADP dti-1354 141 16 its its PRON dti-1354 141 17 energy energy NOUN dti-1354 141 18 requirement requirement NOUN dti-1354 141 19 , , PUNCT dti-1354 141 20 and and CCONJ dti-1354 141 21 bmldh bmldh PROPN dti-1354 141 22 plays play VERB dti-1354 141 23 a a DET dti-1354 141 24 key key ADJ dti-1354 141 25 role.14 role.14 NOUN dti-1354 141 26 the the DET dti-1354 141 27 high high ADJ dti-1354 141 28 catalytic catalytic ADJ dti-1354 141 29 activity activity NOUN dti-1354 141 30 ( ( PUNCT dti-1354 141 31 high high ADJ dti-1354 141 32 kcat kcat NOUN dti-1354 141 33 value value NOUN dti-1354 141 34 ) ) PUNCT dti-1354 141 35 of of ADP dti-1354 141 36 bmldh bmldh PROPN dti-1354 141 37 for for ADP dti-1354 141 38 lactate lactate NOUN dti-1354 141 39 compared compare VERB dti-1354 141 40 to to ADP dti-1354 141 41 b. b. PROPN dti-1354 141 42 bovis bovis PROPN dti-1354 141 43 ldh ldh PROPN dti-1354 141 44 , , PUNCT dti-1354 141 45 p. p. PROPN dti-1354 141 46 falciparum falciparum PROPN dti-1354 142 1 ldh ldh PROPN dti-1354 142 2 , , PUNCT dti-1354 142 3 http://www.la-press.com http://www.la-press.com X dti-1354 143 1 vudriko vudriko ADV dti-1354 143 2 et et NOUN dti-1354 143 3 al al PROPN dti-1354 143 4 36 36 NUM dti-1354 143 5 drug drug NOUN dti-1354 143 6 target target NOUN dti-1354 143 7 insights insight VERB dti-1354 143 8 2014:8 2014:8 NUM dti-1354 143 9 t. t. NOUN dti-1354 143 10 gondii gondii NOUN dti-1354 143 11 , , PUNCT dti-1354 143 12 and and CCONJ dti-1354 143 13 human human ADJ dti-1354 143 14 ldh ldh NOUN dti-1354 143 15 further far ADV dti-1354 143 16 suggests suggest VERB dti-1354 143 17 that that SCONJ dti-1354 143 18 it it PRON dti-1354 143 19 could could AUX dti-1354 143 20 be be AUX dti-1354 143 21 a a DET dti-1354 143 22 novel novel ADJ dti-1354 143 23 candidate candidate NOUN dti-1354 143 24 for for ADP dti-1354 143 25 drug drug NOUN dti-1354 143 26 target.19,28–30 target.19,28–30 PROPN dti-1354 143 27 indeed indeed ADV dti-1354 143 28 , , PUNCT dti-1354 143 29 bmldh bmldh ADJ dti-1354 143 30 inhibitory inhibitory ADJ dti-1354 143 31 assay assay NOUN dti-1354 143 32 showed show VERB dti-1354 143 33 that that SCONJ dti-1354 143 34 the the DET dti-1354 143 35 ic50 ic50 PROPN dti-1354 143 36 value value NOUN dti-1354 143 37 for for ADP dti-1354 143 38 gossypol gossypol PROPN dti-1354 143 39 ( ( PUNCT dti-1354 143 40 a a DET dti-1354 143 41 competitive competitive ADJ dti-1354 143 42 antagonist antagonist NOUN dti-1354 143 43 for for ADP dti-1354 143 44 nad+ nad+ NOUN dti-1354 143 45 ) ) PUNCT dti-1354 143 46 was be AUX dti-1354 143 47 0.345 0.345 NUM dti-1354 143 48     SPACE dti-1354 143 49 µm µm NOUN dti-1354 143 50 . . PUNCT dti-1354 144 1 at at ADP dti-1354 144 2 2.5 2.5 NUM dti-1354 144 3     SPACE dti-1354 144 4 µm µm NOUN dti-1354 144 5 , , PUNCT dti-1354 144 6 catalytic catalytic ADJ dti-1354 144 7 activity activity NOUN dti-1354 144 8 of of ADP dti-1354 144 9 bmldh bmldh PROPN dti-1354 144 10 was be AUX dti-1354 144 11 completely completely ADV dti-1354 144 12 inhibited inhibited ADJ dti-1354 144 13 . . PUNCT dti-1354 145 1 this this DET dti-1354 145 2 finding finding NOUN dti-1354 145 3 is be AUX dti-1354 145 4 consistent consistent ADJ dti-1354 145 5 with with ADP dti-1354 145 6 previous previous ADJ dti-1354 145 7 reports report NOUN dti-1354 145 8 that that SCONJ dti-1354 145 9 ldh ldh PROPN dti-1354 145 10 is be AUX dti-1354 145 11 a a DET dti-1354 145 12 novel novel ADJ dti-1354 145 13 drug drug NOUN dti-1354 145 14 target target NOUN dti-1354 145 15 against against ADP dti-1354 145 16 some some DET dti-1354 145 17 protozoan protozoan NOUN dti-1354 145 18 parasites.15,17–19 parasites.15,17–19 NOUN dti-1354 145 19 since since SCONJ dti-1354 145 20 b. b. PROPN dti-1354 145 21 microti microti PROPN dti-1354 145 22 lacks lack VERB dti-1354 145 23 well well ADV dti-1354 145 24 - - PUNCT dti-1354 145 25 developed develop VERB dti-1354 145 26 tricarboxylic tricarboxylic ADJ dti-1354 145 27 acid acid NOUN dti-1354 145 28 cycle cycle NOUN dti-1354 145 29 , , PUNCT dti-1354 145 30 we we PRON dti-1354 145 31 hypothesize hypothesize VERB dti-1354 145 32 that that SCONJ dti-1354 145 33 inhibition inhibition NOUN dti-1354 145 34 of of ADP dti-1354 145 35 glycolysis glycolysis NOUN dti-1354 145 36 via via ADP dti-1354 145 37 bmldh bmldh PROPN dti-1354 145 38 would would AUX dti-1354 145 39 lead lead VERB dti-1354 145 40 to to ADP dti-1354 145 41 energy energy NOUN dti-1354 145 42 starvation starvation NOUN dti-1354 145 43 and and CCONJ dti-1354 145 44 death death NOUN dti-1354 145 45 of of ADP dti-1354 145 46 the the DET dti-1354 145 47 parasite parasite NOUN dti-1354 145 48 . . PUNCT dti-1354 146 1 however however ADV dti-1354 146 2 , , PUNCT dti-1354 146 3 there there PRON dti-1354 146 4 is be VERB dti-1354 146 5 need need NOUN dti-1354 146 6 for for ADP dti-1354 146 7 further further ADJ dti-1354 146 8 research research NOUN dti-1354 146 9 on on ADP dti-1354 146 10 in in ADP dti-1354 146 11 vivo vivo ADJ dti-1354 146 12 efficacy efficacy NOUN dti-1354 146 13 of of ADP dti-1354 146 14 bmldh bmldh ADJ dti-1354 146 15 inhibitors inhibitor NOUN dti-1354 146 16 in in ADP dti-1354 146 17 b. b. PROPN dti-1354 146 18 microti microti PROPN dti-1354 146 19 - - PUNCT dti-1354 146 20 infected infect VERB dti-1354 146 21 hamsters hamster NOUN dti-1354 146 22 . . PUNCT dti-1354 147 1 such such DET dti-1354 147 2 a a DET dti-1354 147 3 study study NOUN dti-1354 147 4 would would AUX dti-1354 147 5 also also ADV dti-1354 147 6 generate generate VERB dti-1354 147 7 important important ADJ dti-1354 147 8 information information NOUN dti-1354 147 9 on on ADP dti-1354 147 10 the the DET dti-1354 147 11 safety safety NOUN dti-1354 147 12 of of ADP dti-1354 147 13 ldh ldh ADJ dti-1354 147 14 inhibitor inhibitor NOUN dti-1354 147 15 at at ADP dti-1354 147 16 therapeutic therapeutic ADJ dti-1354 147 17 dose dose NOUN dti-1354 147 18 . . PUNCT dti-1354 148 1 while while SCONJ dti-1354 148 2 ldh ldh PROPN dti-1354 148 3 plays play VERB dti-1354 148 4 a a DET dti-1354 148 5 key key ADJ dti-1354 148 6 role role NOUN dti-1354 148 7 in in ADP dti-1354 148 8 glycolysis glycolysis NOUN dti-1354 148 9 in in ADP dti-1354 148 10 mammalian mammalian ADJ dti-1354 148 11 red red ADJ dti-1354 148 12 blood blood NOUN dti-1354 148 13 cells cell NOUN dti-1354 148 14 and and CCONJ dti-1354 148 15 muscles muscle NOUN dti-1354 148 16 , , PUNCT dti-1354 148 17 suppression suppression NOUN dti-1354 148 18 or or CCONJ dti-1354 148 19 deficiency deficiency NOUN dti-1354 148 20 of of ADP dti-1354 148 21 ldh ldh PROPN dti-1354 148 22 causes cause VERB dti-1354 148 23 benign benign ADJ dti-1354 148 24 conditions condition NOUN dti-1354 148 25 with with ADP dti-1354 148 26 no no DET dti-1354 148 27 or or CCONJ dti-1354 148 28 minimum minimum ADJ dti-1354 148 29 adverse adverse ADJ dti-1354 148 30 effects.31,32 effects.31,32 NOUN dti-1354 148 31 merkle merkle NOUN dti-1354 148 32 and and CCONJ dti-1354 148 33 others33 others33 NOUN dti-1354 148 34 have have AUX dti-1354 148 35 also also ADV dti-1354 148 36 reported report VERB dti-1354 148 37 that that SCONJ dti-1354 148 38 up up ADP dti-1354 148 39 to to PART dti-1354 148 40 50 50 NUM dti-1354 148 41 % % NOUN dti-1354 148 42 reduction reduction NOUN dti-1354 148 43 of of ADP dti-1354 148 44 ldh ldh NOUN dti-1354 148 45 - - PUNCT dti-1354 148 46 a a DET dti-1354 148 47 activity activity NOUN dti-1354 148 48 in in ADP dti-1354 148 49 mice mouse NOUN dti-1354 148 50 produced produce VERB dti-1354 148 51 no no DET dti-1354 148 52 obvious obvious ADJ dti-1354 148 53 harm harm NOUN dti-1354 148 54 . . PUNCT dti-1354 149 1 this this PRON dti-1354 149 2 probably probably ADV dti-1354 149 3 explains explain VERB dti-1354 149 4 why why SCONJ dti-1354 149 5 there there PRON dti-1354 149 6 is be VERB dti-1354 149 7 a a DET dti-1354 149 8 growing grow VERB dti-1354 149 9 momentum momentum NOUN dti-1354 149 10 in in ADP dti-1354 149 11 the the DET dti-1354 149 12 synthesis synthesis NOUN dti-1354 149 13 of of ADP dti-1354 149 14 new new ADJ dti-1354 149 15 ldh ldh NOUN dti-1354 149 16 inhibitors inhibitor NOUN dti-1354 149 17 as as ADP dti-1354 149 18 potential potential ADJ dti-1354 149 19 drug drug NOUN dti-1354 149 20 against against ADP dti-1354 149 21 some some DET dti-1354 149 22 protozoan protozoan NOUN dti-1354 149 23 organisms20 organisms20 ADJ dti-1354 149 24 and and CCONJ dti-1354 149 25 their their PRON dti-1354 149 26 potential potential ADJ dti-1354 149 27 use use NOUN dti-1354 149 28 as as ADP dti-1354 149 29 anti anti ADJ dti-1354 149 30 - - ADJ dti-1354 149 31 tumor tumor ADJ dti-1354 149 32 drugs.34,35 drugs.34,35 ADJ dti-1354 149 33 conclusion conclusion NOUN dti-1354 149 34 this this DET dti-1354 149 35 study study NOUN dti-1354 149 36 revealed reveal VERB dti-1354 149 37 that that SCONJ dti-1354 149 38 bmldh bmldh PROPN dti-1354 149 39 is be AUX dti-1354 149 40 a a DET dti-1354 149 41 36 36 NUM dti-1354 149 42     SPACE dti-1354 149 43 kda kda NOUN dti-1354 149 44 protein protein NOUN dti-1354 149 45 that that PRON dti-1354 149 46 has have VERB dti-1354 149 47 nucleoplasmin nucleoplasmin NOUN dti-1354 149 48 localization localization NOUN dti-1354 149 49 . . PUNCT dti-1354 150 1 the the DET dti-1354 150 2 kinetic kinetic ADJ dti-1354 150 3 parameters parameter NOUN dti-1354 150 4 of of ADP dti-1354 150 5 bmld bmld PROPN dti-1354 150 6 also also ADV dti-1354 150 7 suggest suggest VERB dti-1354 150 8 that that SCONJ dti-1354 150 9 the the DET dti-1354 150 10 enzyme enzyme NOUN dti-1354 150 11 has have VERB dti-1354 150 12 a a DET dti-1354 150 13 very very ADV dti-1354 150 14 high high ADJ dti-1354 150 15 catalytic catalytic ADJ dti-1354 150 16 activity activity NOUN dti-1354 150 17 compared compare VERB dti-1354 150 18 to to ADP dti-1354 150 19 other other ADJ dti-1354 150 20 apicomplexan apicomplexan PROPN dti-1354 150 21 ldh ldh PROPN dti-1354 150 22 ; ; PUNCT dti-1354 150 23 moreover moreover ADV dti-1354 150 24 , , PUNCT dti-1354 150 25 its its PRON dti-1354 150 26 activity activity NOUN dti-1354 150 27 can can AUX dti-1354 150 28 be be AUX dti-1354 150 29 inhibited inhibit VERB dti-1354 150 30 at at ADP dti-1354 150 31 low low ADJ dti-1354 150 32 concentration concentration NOUN dti-1354 150 33 of of ADP dti-1354 150 34 nad+ nad+ PROPN dti-1354 150 35 analog analog NOUN dti-1354 150 36 , , PUNCT dti-1354 150 37 gossypol gossypol PROPN dti-1354 150 38 . . PUNCT dti-1354 151 1 we we PRON dti-1354 151 2 therefore therefore ADV dti-1354 151 3 propose propose VERB dti-1354 151 4 further further ADJ dti-1354 151 5 studies study NOUN dti-1354 151 6 to to PART dti-1354 151 7 explore explore VERB dti-1354 151 8 the the DET dti-1354 151 9 in in ADP dti-1354 151 10 vivo vivo ADJ dti-1354 151 11 efficacy efficacy NOUN dti-1354 151 12 of of ADP dti-1354 151 13 bmlh bmlh ADJ dti-1354 151 14 inhibitor inhibitor NOUN dti-1354 151 15 in in ADP dti-1354 151 16 hamster hamster PROPN dti-1354 151 17 model model PROPN dti-1354 151 18 . . PUNCT dti-1354 152 1 acknowledgment acknowledgment NOUN dti-1354 152 2 we we PRON dti-1354 152 3 acknowledge acknowledge VERB dti-1354 152 4 the the DET dti-1354 152 5 technical technical ADJ dti-1354 152 6 contributions contribution NOUN dti-1354 152 7 made make VERB dti-1354 152 8 by by ADP dti-1354 152 9 members member NOUN dti-1354 152 10 of of ADP dti-1354 152 11 the the DET dti-1354 152 12 host host NOUN dti-1354 152 13 defense defense NOUN dti-1354 152 14 , , PUNCT dti-1354 152 15 babesia babesia NOUN dti-1354 152 16 and and CCONJ dti-1354 152 17 vaccine vaccine NOUN dti-1354 152 18 laboratories laboratory NOUN dti-1354 152 19 , , PUNCT dti-1354 152 20 national national ADJ dti-1354 152 21 research research NOUN dti-1354 152 22 center center NOUN dti-1354 152 23 for for ADP dti-1354 152 24 protozoan protozoan NOUN dti-1354 152 25 diseases disease NOUN dti-1354 152 26 ( ( PUNCT dti-1354 152 27 nrcpd nrcpd PROPN dti-1354 152 28 ) ) PUNCT dti-1354 152 29 , , PUNCT dti-1354 152 30 obihiro obihiro PROPN dti-1354 152 31 university university PROPN dti-1354 152 32 of of ADP dti-1354 152 33 agriculture agriculture NOUN dti-1354 152 34 and and CCONJ dti-1354 152 35 veterinary veterinary ADJ dti-1354 152 36 medicine medicine NOUN dti-1354 152 37 . . PUNCT dti-1354 153 1 the the DET dti-1354 153 2 contributions contribution NOUN dti-1354 153 3 offered offer VERB dti-1354 153 4 by by ADP dti-1354 153 5 dennis dennis PROPN dti-1354 153 6 table table PROPN dti-1354 153 7 1 1 NUM dti-1354 153 8 . . PUNCT dti-1354 154 1 kinetic kinetic ADJ dti-1354 154 2 parameters parameter NOUN dti-1354 154 3 for for ADP dti-1354 154 4 bmldh bmldh ADJ dti-1354 154 5 . . PUNCT dti-1354 155 1 cofactor cofactor NOUN dti-1354 155 2 / / SYM dti-1354 155 3 substrate substrate NOUN dti-1354 155 4 km km NOUN dti-1354 155 5 ( ( PUNCT dti-1354 155 6 mm mm NOUN dti-1354 155 7 ) ) PUNCT dti-1354 155 8 vmax vmax PROPN dti-1354 155 9 ( ( PUNCT dti-1354 155 10 mm mm PROPN dti-1354 155 11 / / SYM dti-1354 155 12 min min NOUN dti-1354 155 13 ) ) PUNCT dti-1354 155 14 kcat kcat NOUN dti-1354 155 15 ( ( PUNCT dti-1354 155 16 min-1 min-1 NOUN dti-1354 155 17 ) ) PUNCT dti-1354 155 18 kcat kcat PROPN dti-1354 155 19 / / SYM dti-1354 155 20 km km PROPN dti-1354 155 21 ( ( PUNCT dti-1354 155 22 m-1 m-1 X dti-1354 155 23 min-1 min-1 X dti-1354 155 24 ) ) PUNCT dti-1354 155 25 nad+ nad+ PROPN dti-1354 155 26 8.76 8.76 NUM dti-1354 155 27 ± ± NUM dti-1354 155 28     SPACE dti-1354 155 29 0.57 0.57 NUM dti-1354 155 30 0.008765 0.008765 NUM dti-1354 155 31 ± ± NUM dti-1354 155 32 0.00057 0.00057 NUM dti-1354 155 33 6.31 6.31 NUM dti-1354 155 34 × × NOUN dti-1354 155 35     SPACE dti-1354 155 36 105 105 NUM dti-1354 155 37 5.126 5.126 NUM dti-1354 155 38 × × NOUN dti-1354 155 39     SPACE dti-1354 155 40 108 108 NUM dti-1354 155 41 lactate lactate PROPN dti-1354 155 42 99.96 99.96 NUM dti-1354 155 43 ± ± NUM dti-1354 155 44     SPACE dti-1354 155 45 22.33 22.33 NUM dti-1354 155 46 0.01014 0.01014 NUM dti-1354 155 47 ± ± NUM dti-1354 155 48 0.00077 0.00077 NUM dti-1354 155 49 7.3 7.3 NUM dti-1354 155 50 × × NOUN dti-1354 155 51     SPACE dti-1354 155 52 105 105 NUM dti-1354 155 53 7.3003 7.3003 NUM dti-1354 155 54 × × NOUN dti-1354 155 55     SPACE dti-1354 155 56 106 106 NUM dti-1354 155 57 figure figure NOUN dti-1354 155 58 5 5 NUM dti-1354 155 59 . . PUNCT dti-1354 156 1 ( ( PUNCT dti-1354 156 2 a a X dti-1354 156 3 ) ) PUNCT dti-1354 156 4 effect effect NOUN dti-1354 156 5 of of ADP dti-1354 156 6 ph ph NOUN dti-1354 156 7 on on ADP dti-1354 156 8 catalytic catalytic ADJ dti-1354 156 9 efficiency efficiency NOUN dti-1354 156 10 of of ADP dti-1354 156 11 bmldh bmldh ADJ dti-1354 156 12 . . PUNCT dti-1354 157 1 ( ( PUNCT dti-1354 157 2 b b X dti-1354 157 3 ) ) PUNCT dti-1354 157 4 michaelis michaeli NOUN dti-1354 157 5 – – PUNCT dti-1354 157 6 menten menten ADJ dti-1354 157 7 curve curve NOUN dti-1354 157 8 for for ADP dti-1354 157 9 nad+ nad+ PROPN dti-1354 157 10 ( ( PUNCT dti-1354 157 11 co co NOUN dti-1354 157 12 - - NOUN dti-1354 157 13 factor factor NOUN dti-1354 157 14 ) ) PUNCT dti-1354 157 15 at at ADP dti-1354 157 16 100 100 NUM dti-1354 157 17 mm mm NOUN dti-1354 157 18 lactate lactate NOUN dti-1354 157 19 . . PUNCT dti-1354 158 1 ( ( PUNCT dti-1354 158 2 c c X dti-1354 158 3 ) ) PUNCT dti-1354 158 4 michaelis michaeli NOUN dti-1354 158 5 – – PUNCT dti-1354 158 6 menten menten VERB dti-1354 158 7 curve curve NOUN dti-1354 158 8 for for ADP dti-1354 158 9 lactate lactate NOUN dti-1354 158 10 ( ( PUNCT dti-1354 158 11 substrate substrate NOUN dti-1354 158 12 ) ) PUNCT dti-1354 158 13 at at ADP dti-1354 158 14 2.4 2.4 NUM dti-1354 158 15 mm mm NOUN dti-1354 158 16 nad+ nad+ NOUN dti-1354 158 17 . . PUNCT dti-1354 159 1 ( ( PUNCT dti-1354 159 2 d d X dti-1354 159 3 ) ) PUNCT dti-1354 159 4 non non ADJ dti-1354 159 5 - - ADJ dti-1354 159 6 linear linear ADJ dti-1354 159 7 regression regression NOUN dti-1354 159 8 curve curve NOUN dti-1354 159 9 for for ADP dti-1354 159 10 inhibition inhibition NOUN dti-1354 159 11 of of ADP dti-1354 159 12 bmldh bmldh ADJ dti-1354 159 13 by by ADP dti-1354 159 14 gossypol gossypol PROPN dti-1354 159 15 . . PUNCT dti-1354 160 1 http://www.la-press.com http://www.la-press.com X dti-1354 161 1 molecular molecular ADJ dti-1354 161 2 and and CCONJ dti-1354 161 3 kinetic kinetic ADJ dti-1354 161 4 characterization characterization NOUN dti-1354 161 5 of of ADP dti-1354 161 6 b. b. PROPN dti-1354 161 7 microti microti PROPN dti-1354 161 8 ldh ldh PROPN dti-1354 161 9 37drug 37drug PROPN dti-1354 161 10 target target NOUN dti-1354 161 11 insights insight NOUN dti-1354 161 12 2014:8 2014:8 NUM dti-1354 161 13 muhanguzi muhanguzi PROPN dti-1354 161 14 , , PUNCT dti-1354 161 15 maki maki PROPN dti-1354 161 16 ishiwata ishiwata PROPN dti-1354 161 17 , , PUNCT dti-1354 161 18 miki miki PROPN dti-1354 161 19 - - PUNCT dti-1354 161 20 araki araki PROPN dti-1354 161 21 , , PUNCT dti-1354 161 22 and and CCONJ dti-1354 161 23 ken ken PROPN dti-1354 161 24 saito saito PROPN dti-1354 161 25 are be AUX dti-1354 161 26 acknowledged acknowledge VERB dti-1354 161 27 . . PUNCT dti-1354 162 1 author author NOUN dti-1354 162 2 contributions contribution NOUN dti-1354 162 3 pv pv INTJ dti-1354 162 4 , , PUNCT dti-1354 162 5 tm tm PROPN dti-1354 162 6 , , PUNCT dti-1354 162 7 sc sc PROPN dti-1354 162 8 , , PUNCT dti-1354 162 9 mat mat PROPN dti-1354 162 10 , , PUNCT dti-1354 162 11 aam aam PROPN dti-1354 162 12 , , PUNCT dti-1354 162 13 kk kk PROPN dti-1354 162 14 , , PUNCT dti-1354 162 15 yn yn PROPN dti-1354 162 16 , , PUNCT dti-1354 162 17 and and CCONJ dti-1354 162 18 xx xx PRON dti-1354 162 19 conceived conceive VERB dti-1354 162 20 and and CCONJ dti-1354 162 21 designed design VERB dti-1354 162 22 the the DET dti-1354 162 23 experiments experiment NOUN dti-1354 162 24 . . PUNCT dti-1354 163 1 pv pv INTJ dti-1354 163 2 , , PUNCT dti-1354 163 3 sc sc PROPN dti-1354 163 4 , , PUNCT dti-1354 163 5 tm tm PROPN dti-1354 163 6 , , PUNCT dti-1354 163 7 pfam pfam NOUN dti-1354 163 8 , , PUNCT dti-1354 163 9 and and CCONJ dti-1354 163 10 mat mat NOUN dti-1354 163 11 analyzed analyze VERB dti-1354 163 12 the the DET dti-1354 163 13 data datum NOUN dti-1354 163 14 . . PUNCT dti-1354 164 1 pv pv INTJ dti-1354 164 2 , , PUNCT dti-1354 164 3 sc sc PROPN dti-1354 164 4 , , PUNCT dti-1354 164 5 and and CCONJ dti-1354 164 6 xx xx PRON dti-1354 164 7 wrote write VERB dti-1354 164 8 the the DET dti-1354 164 9 first first ADJ dti-1354 164 10 draft draft NOUN dti-1354 164 11 of of ADP dti-1354 164 12 the the DET dti-1354 164 13 manuscript manuscript NOUN dti-1354 164 14 . . PUNCT dti-1354 165 1 pv pv INTJ dti-1354 165 2 , , PUNCT dti-1354 165 3 tm tm PROPN dti-1354 165 4 , , PUNCT dti-1354 165 5 sc sc PROPN dti-1354 165 6 , , PUNCT dti-1354 165 7 aam aam PROPN dti-1354 165 8 , , PUNCT dti-1354 165 9 pfam pfam PROPN dti-1354 165 10 , , PUNCT dti-1354 165 11 kk kk PROPN dti-1354 165 12 , , PUNCT dti-1354 165 13 and and CCONJ dti-1354 165 14 xx xx PRON dti-1354 165 15 contributed contribute VERB dti-1354 165 16 to to ADP dti-1354 165 17 the the DET dti-1354 165 18 writing writing NOUN dti-1354 165 19 of of ADP dti-1354 165 20 the the DET dti-1354 165 21 manuscript manuscript NOUN dti-1354 165 22 . . PUNCT dti-1354 166 1 pv pv INTJ dti-1354 166 2 , , PUNCT dti-1354 166 3 tm tm PROPN dti-1354 166 4 , , PUNCT dti-1354 166 5 sc sc PROPN dti-1354 166 6 , , PUNCT dti-1354 166 7 mat mat PROPN dti-1354 166 8 , , PUNCT dti-1354 166 9 aam aam PROPN dti-1354 166 10 , , PUNCT dti-1354 166 11 kk kk PROPN dti-1354 166 12 , , PUNCT dti-1354 166 13 pfam pfam NOUN dti-1354 166 14 , , PUNCT dti-1354 166 15 yn yn PROPN dti-1354 166 16 , , PUNCT dti-1354 166 17 and and CCONJ dti-1354 166 18 xx xx PRON dti-1354 166 19 agree agree VERB dti-1354 166 20 with with ADP dti-1354 166 21 manuscript manuscript NOUN dti-1354 166 22 results result NOUN dti-1354 166 23 and and CCONJ dti-1354 166 24 conclusions conclusion NOUN dti-1354 166 25 . . PUNCT dti-1354 167 1 pv pv INTJ dti-1354 167 2 , , PUNCT dti-1354 167 3 sc sc PROPN dti-1354 167 4 , , PUNCT dti-1354 167 5 tm tm NOUN dti-1354 167 6 , , PUNCT dti-1354 167 7 and and CCONJ dti-1354 167 8 xx xx PRON dti-1354 167 9 jointly jointly ADV dti-1354 167 10 developed develop VERB dti-1354 167 11 the the DET dti-1354 167 12 structure structure NOUN dti-1354 167 13 and and CCONJ dti-1354 167 14 arguments argument NOUN dti-1354 167 15 for for ADP dti-1354 167 16 the the DET dti-1354 167 17 paper paper NOUN dti-1354 167 18 . . PUNCT dti-1354 168 1 mat mat NOUN dti-1354 168 2 and and CCONJ dti-1354 168 3 xx xx NUM dti-1354 168 4 made make VERB dti-1354 168 5 critical critical ADJ dti-1354 168 6 revisions revision NOUN dti-1354 168 7 and and CCONJ dti-1354 168 8 approved approve VERB dti-1354 168 9 final final ADJ dti-1354 168 10 version version NOUN dti-1354 168 11 . . PUNCT dti-1354 169 1 all all DET dti-1354 169 2 authors author NOUN dti-1354 169 3 reviewed review VERB dti-1354 169 4 and and CCONJ dti-1354 169 5 approved approve VERB dti-1354 169 6 the the DET dti-1354 169 7 final final ADJ dti-1354 169 8 manuscript manuscript NOUN dti-1354 169 9 . . PUNCT dti-1354 170 1 references reference NOUN dti-1354 170 2 1 1 NUM dti-1354 170 3 . . PUNCT dti-1354 170 4 hunfeld hunfeld PROPN dti-1354 170 5 kp kp PROPN dti-1354 170 6 , , PUNCT dti-1354 170 7 hildebrandt hildebrandt PROPN dti-1354 170 8 a a PRON dti-1354 170 9 , , PUNCT dti-1354 170 10 gray gray ADJ dti-1354 170 11 js js PROPN dti-1354 170 12 . . PUNCT dti-1354 171 1 babesiosis babesiosis NOUN dti-1354 171 2 : : PUNCT dti-1354 171 3 recent recent ADJ dti-1354 171 4 insights insight NOUN dti-1354 171 5 into into ADP dti-1354 171 6 an an DET dti-1354 171 7 ancient ancient ADJ dti-1354 171 8 disease disease NOUN dti-1354 171 9 . . PUNCT dti-1354 172 1 int int NOUN dti-1354 172 2 j j PROPN dti-1354 172 3 parasitol parasitol NOUN dti-1354 172 4 . . PUNCT dti-1354 173 1 2008;38(11):1219–1237 2008;38(11):1219–1237 X dti-1354 173 2 . . PUNCT dti-1354 174 1 2 2 X dti-1354 174 2 . . X dti-1354 174 3 nakajima nakajima PROPN dti-1354 174 4 r r PROPN dti-1354 174 5 , , PUNCT dti-1354 174 6 tsuji tsuji PROPN dti-1354 174 7 m m PROPN dti-1354 174 8 , , PUNCT dti-1354 174 9 oda oda PROPN dti-1354 174 10 k k PROPN dti-1354 174 11 , , PUNCT dti-1354 174 12 et et PROPN dti-1354 174 13 al al PROPN dti-1354 174 14 . . PUNCT dti-1354 175 1 babesia babesia PROPN dti-1354 175 2 microti microti PROPN dti-1354 175 3 - - PUNCT dti-1354 175 4 group group NOUN dti-1354 175 5 parasites parasite NOUN dti-1354 175 6 compared compare VERB dti-1354 175 7 phylogenetically phylogenetically ADV dti-1354 175 8 by by ADP dti-1354 175 9 complete complete ADJ dti-1354 175 10 sequencing sequencing NOUN dti-1354 175 11 of of ADP dti-1354 175 12 the the DET dti-1354 175 13 ccteta ccteta NOUN dti-1354 175 14 gene gene NOUN dti-1354 175 15 in in ADP dti-1354 175 16 36 36 NUM dti-1354 175 17 isolates isolate NOUN dti-1354 175 18 . . PUNCT dti-1354 176 1 j j PROPN dti-1354 176 2 vet vet PROPN dti-1354 176 3 med med PROPN dti-1354 176 4 sci sci PROPN dti-1354 176 5 . . PUNCT dti-1354 177 1 2009;71(1):55–68 2009;71(1):55–68 X dti-1354 177 2 . . PUNCT dti-1354 178 1 3 3 X dti-1354 178 2 . . X dti-1354 178 3 hersh hersh PROPN dti-1354 178 4 mh mh PROPN dti-1354 178 5 , , PUNCT dti-1354 178 6 tibbetts tibbetts PROPN dti-1354 178 7 m m PROPN dti-1354 178 8 , , PUNCT dti-1354 178 9 strauss strauss PROPN dti-1354 178 10 m m PROPN dti-1354 178 11 , , PUNCT dti-1354 178 12 ostfeld ostfeld NOUN dti-1354 178 13 rs rs PROPN dti-1354 178 14 , , PUNCT dti-1354 178 15 keesing keese VERB dti-1354 178 16 f. f. PROPN dti-1354 178 17 reservoir reservoir PROPN dti-1354 178 18 competence competence NOUN dti-1354 178 19 of of ADP dti-1354 178 20 wildlife wildlife NOUN dti-1354 178 21 host host NOUN dti-1354 178 22 species specie NOUN dti-1354 178 23 for for ADP dti-1354 178 24 babesia babesia NOUN dti-1354 178 25 microti microti PROPN dti-1354 178 26 . . PUNCT dti-1354 179 1 emerg emerg PROPN dti-1354 179 2 infect infect PROPN dti-1354 179 3 dis dis PROPN dti-1354 179 4 . . PUNCT dti-1354 180 1 2012;18(12):1951 2012;18(12):1951 NUM dti-1354 180 2 . . NOUN dti-1354 180 3 4 4 NUM dti-1354 180 4 . . X dti-1354 180 5 zamoto zamoto NOUN dti-1354 180 6 a a PROPN dti-1354 180 7 , , PUNCT dti-1354 180 8 tsuji tsuji PROPN dti-1354 180 9 m m PROPN dti-1354 180 10 , , PUNCT dti-1354 180 11 wei wei PROPN dti-1354 180 12 q q PROPN dti-1354 180 13 , , PUNCT dti-1354 180 14 et et PROPN dti-1354 180 15 al al PROPN dti-1354 180 16 . . PUNCT dti-1354 180 17 epizootiologic epizootiologic ADJ dti-1354 180 18 survey survey NOUN dti-1354 180 19 for for ADP dti-1354 180 20 babesia babesia NOUN dti-1354 180 21 microti microti PROPN dti-1354 180 22 among among ADP dti-1354 180 23 small small ADJ dti-1354 180 24 wild wild ADJ dti-1354 180 25 mammals mammal NOUN dti-1354 180 26 in in ADP dti-1354 180 27 northeastern northeastern ADJ dti-1354 180 28 eurasia eurasia PROPN dti-1354 180 29 and and CCONJ dti-1354 180 30 a a DET dti-1354 180 31 geographic geographic ADJ dti-1354 180 32 diversity diversity NOUN dti-1354 180 33 in in ADP dti-1354 180 34 the the DET dti-1354 180 35 beta beta NOUN dti-1354 180 36 - - PUNCT dti-1354 180 37 tubulin tubulin NOUN dti-1354 180 38 gene gene NOUN dti-1354 180 39 sequences sequence NOUN dti-1354 180 40 . . PUNCT dti-1354 181 1 j j PROPN dti-1354 181 2 vet vet PROPN dti-1354 181 3 med med PROPN dti-1354 181 4 sci sci PROPN dti-1354 181 5 . . PUNCT dti-1354 182 1 2004;66(7):785–792 2004;66(7):785–792 NUM dti-1354 182 2 . . X dti-1354 182 3 5 5 NUM dti-1354 182 4 . . X dti-1354 182 5 hildebrandt hildebrandt PROPN dti-1354 182 6 a a PRON dti-1354 182 7 , , PUNCT dti-1354 182 8 hunfeld hunfeld PROPN dti-1354 182 9 kp kp PROPN dti-1354 182 10 , , PUNCT dti-1354 182 11 baier baier PROPN dti-1354 182 12 m m PROPN dti-1354 182 13 , , PUNCT dti-1354 182 14 et et PROPN dti-1354 182 15 al al PROPN dti-1354 182 16 . . PROPN dti-1354 183 1 first first PROPN dti-1354 183 2 confirmed confirm VERB dti-1354 183 3 autochthonous autochthonous ADJ dti-1354 183 4 case case NOUN dti-1354 183 5 of of ADP dti-1354 183 6 human human ADJ dti-1354 183 7 babesia babesia NOUN dti-1354 183 8 microti microti VERB dti-1354 183 9 infection infection NOUN dti-1354 183 10 in in ADP dti-1354 183 11 europe europe PROPN dti-1354 183 12 . . PUNCT dti-1354 184 1 eur eur PROPN dti-1354 184 2 j j PROPN dti-1354 184 3 clin clin PROPN dti-1354 184 4 microbiol microbiol PROPN dti-1354 184 5 infect infect VERB dti-1354 184 6 dis dis PROPN dti-1354 184 7 . . PUNCT dti-1354 185 1 2007 2007 NUM dti-1354 185 2 ; ; PUNCT dti-1354 185 3 26(8):595–601 26(8):595–601 NUM dti-1354 185 4 . . NOUN dti-1354 185 5 6 6 NUM dti-1354 185 6 . . X dti-1354 185 7 gray gray PROPN dti-1354 185 8 j j PROPN dti-1354 185 9 , , PUNCT dti-1354 185 10 zintl zintl PROPN dti-1354 185 11 a a X dti-1354 185 12 , , PUNCT dti-1354 185 13 hildebrandt hildebrandt PROPN dti-1354 185 14 a a PRON dti-1354 185 15 , , PUNCT dti-1354 185 16 hunfeld hunfeld PROPN dti-1354 185 17 kp kp PROPN dti-1354 185 18 , , PUNCT dti-1354 185 19 weiss weiss PROPN dti-1354 185 20 l. l. PROPN dti-1354 185 21 zoonotic zoonotic PROPN dti-1354 185 22 babesiosis babesiosis NOUN dti-1354 185 23 : : PUNCT dti-1354 185 24 overview overview NOUN dti-1354 185 25 of of ADP dti-1354 185 26 the the DET dti-1354 185 27 disease disease NOUN dti-1354 185 28 and and CCONJ dti-1354 185 29 novel novel ADJ dti-1354 185 30 aspects aspect NOUN dti-1354 185 31 of of ADP dti-1354 185 32 pathogen pathogen NOUN dti-1354 185 33 identity identity NOUN dti-1354 185 34 . . PUNCT dti-1354 186 1 ticks tick NOUN dti-1354 186 2 tick tick VERB dti-1354 186 3 borne bear VERB dti-1354 186 4 dis dis PROPN dti-1354 186 5 . . PUNCT dti-1354 187 1 2010;1(1):3–10 2010;1(1):3–10 NUM dti-1354 187 2 . . X dti-1354 187 3 7 7 X dti-1354 187 4 . . X dti-1354 187 5 schnittger schnittger NOUN dti-1354 187 6 l l PROPN dti-1354 187 7 , , PUNCT dti-1354 187 8 rodriguez rodriguez PROPN dti-1354 187 9 ae ae PROPN dti-1354 187 10 , , PUNCT dti-1354 187 11 florin florin PROPN dti-1354 187 12 - - PUNCT dti-1354 187 13 christensen christensen PROPN dti-1354 187 14 m m PROPN dti-1354 187 15 , , PUNCT dti-1354 187 16 et et PROPN dti-1354 187 17 al al PROPN dti-1354 187 18 . . PUNCT dti-1354 187 19 babesia babesia PROPN dti-1354 187 20 : : PUNCT dti-1354 187 21 a a DET dti-1354 187 22 world world NOUN dti-1354 187 23 emerging emerge VERB dti-1354 187 24 . . PUNCT dti-1354 188 1 infect infect VERB dti-1354 188 2 genet genet NOUN dti-1354 188 3 evol evol NOUN dti-1354 188 4 . . PUNCT dti-1354 189 1 2012;12(8):1788–1809 2012;12(8):1788–1809 NUM dti-1354 189 2 . . PUNCT dti-1354 190 1 8 8 NUM dti-1354 190 2 . . X dti-1354 190 3 gubernot gubernot PROPN dti-1354 190 4 dm dm PROPN dti-1354 190 5 , , PUNCT dti-1354 190 6 lucey lucey PROPN dti-1354 190 7 ct ct PROPN dti-1354 190 8 , , PUNCT dti-1354 190 9 lee lee PROPN dti-1354 190 10 kc kc PROPN dti-1354 190 11 , , PUNCT dti-1354 190 12 conley conley PROPN dti-1354 190 13 gb gb PROPN dti-1354 190 14 , , PUNCT dti-1354 190 15 holness holness ADJ dti-1354 190 16 lg lg NOUN dti-1354 190 17 , , PUNCT dti-1354 190 18 wise wise ADJ dti-1354 190 19 rp rp NOUN dti-1354 190 20 . . PUNCT dti-1354 191 1 babesia babesia PROPN dti-1354 191 2 infection infection NOUN dti-1354 191 3 through through ADP dti-1354 191 4 blood blood NOUN dti-1354 191 5 transfusions transfusion NOUN dti-1354 191 6 : : PUNCT dti-1354 191 7 reports report NOUN dti-1354 191 8 received receive VERB dti-1354 191 9 by by ADP dti-1354 191 10 the the DET dti-1354 191 11 us us PROPN dti-1354 191 12 food food NOUN dti-1354 191 13 and and CCONJ dti-1354 191 14 drug drug NOUN dti-1354 191 15 administration administration NOUN dti-1354 191 16 , , PUNCT dti-1354 191 17 1997–2007 1997–2007 NUM dti-1354 191 18 . . PUNCT dti-1354 192 1 clin clin PROPN dti-1354 192 2 infect infect PROPN dti-1354 192 3 dis dis PROPN dti-1354 192 4 . . PUNCT dti-1354 193 1 2009;48(1):25–30 2009;48(1):25–30 X dti-1354 193 2 . . NOUN dti-1354 193 3 9 9 NUM dti-1354 193 4 . . PUNCT dti-1354 193 5 mcquiston mcquiston PROPN dti-1354 193 6 j j PROPN dti-1354 193 7 , , PUNCT dti-1354 193 8 childs child VERB dti-1354 193 9 j j PROPN dti-1354 193 10 , , PUNCT dti-1354 193 11 chamberland chamberland NOUN dti-1354 193 12 m m PROPN dti-1354 193 13 , , PUNCT dti-1354 193 14 et et PROPN dti-1354 193 15 al al PROPN dti-1354 193 16 . . PUNCT dti-1354 194 1 transmission transmission NOUN dti-1354 194 2 of of ADP dti-1354 194 3 tick tick NOUN dti-1354 194 4 - - PUNCT dti-1354 194 5 borne bear VERB dti-1354 194 6 agents agent NOUN dti-1354 194 7 of of ADP dti-1354 194 8 disease disease NOUN dti-1354 194 9 by by ADP dti-1354 194 10 blood blood NOUN dti-1354 194 11 transfusion transfusion NOUN dti-1354 194 12 : : PUNCT dti-1354 194 13 a a DET dti-1354 194 14 review review NOUN dti-1354 194 15 of of ADP dti-1354 194 16 known known ADJ dti-1354 194 17 and and CCONJ dti-1354 194 18 potential potential ADJ dti-1354 194 19 risks risk NOUN dti-1354 194 20 in in ADP dti-1354 194 21 the the DET dti-1354 194 22 united united PROPN dti-1354 194 23 states states PROPN dti-1354 194 24 . . PUNCT dti-1354 195 1 transfusion transfusion NOUN dti-1354 195 2 . . PUNCT dti-1354 196 1 2000;40(3):274–284 2000;40(3):274–284 NUM dti-1354 196 2 . . PROPN dti-1354 197 1 10 10 NUM dti-1354 197 2 . . PUNCT dti-1354 198 1 sweeney sweeney PROPN dti-1354 198 2 cj cj PROPN dti-1354 198 3 , , PUNCT dti-1354 198 4 ghassemi ghassemi PROPN dti-1354 198 5 m m PROPN dti-1354 198 6 , , PUNCT dti-1354 198 7 agger agger PROPN dti-1354 198 8 wa wa PROPN dti-1354 198 9 , , PUNCT dti-1354 198 10 persing perse VERB dti-1354 198 11 dh dh PROPN dti-1354 198 12 . . PUNCT dti-1354 199 1 coinfection coinfection NOUN dti-1354 199 2 with with ADP dti-1354 199 3 babesia babesia PROPN dti-1354 199 4 microti microti PROPN dti-1354 199 5 and and CCONJ dti-1354 199 6 borrelia borrelia NOUN dti-1354 199 7 burgdorferi burgdorferi NOUN dti-1354 199 8 in in ADP dti-1354 199 9 a a DET dti-1354 199 10 western western ADJ dti-1354 199 11 wisconsin wisconsin PROPN dti-1354 199 12 resident resident NOUN dti-1354 199 13 . . PUNCT dti-1354 200 1 mayo mayo PROPN dti-1354 200 2 clin clin PROPN dti-1354 200 3 proc proc PROPN dti-1354 200 4 . . PUNCT dti-1354 201 1 1998;73(4):338–341 1998;73(4):338–341 NUM dti-1354 201 2 . . NOUN dti-1354 201 3 11 11 NUM dti-1354 201 4 . . PUNCT dti-1354 201 5 krause krause PROPN dti-1354 201 6 pj pj PROPN dti-1354 201 7 , , PUNCT dti-1354 201 8 gewurz gewurz PROPN dti-1354 201 9 be be VERB dti-1354 201 10 , , PUNCT dti-1354 201 11 hill hill PROPN dti-1354 201 12 d d PROPN dti-1354 201 13 , , PUNCT dti-1354 201 14 et et PROPN dti-1354 201 15 al al PROPN dti-1354 201 16 . . PROPN dti-1354 201 17 persistent persistent ADJ dti-1354 201 18 and and CCONJ dti-1354 201 19 relapsing relapse VERB dti-1354 201 20 babesiosis babesiosis NOUN dti-1354 201 21 in in ADP dti-1354 201 22 immunocompromised immunocompromise VERB dti-1354 201 23 patients patient NOUN dti-1354 201 24 . . PUNCT dti-1354 202 1 clin clin PROPN dti-1354 202 2 infect infect PROPN dti-1354 202 3 dis dis PROPN dti-1354 202 4 . . PUNCT dti-1354 203 1 2008;46(3):370–376 2008;46(3):370–376 X dti-1354 203 2 . . PROPN dti-1354 203 3 12 12 NUM dti-1354 203 4 . . PUNCT dti-1354 204 1 krause krause PROPN dti-1354 204 2 pj pj PROPN dti-1354 204 3 , , PUNCT dti-1354 204 4 lepore lepore PROPN dti-1354 204 5 t t PROPN dti-1354 204 6 , , PUNCT dti-1354 204 7 sikand sikand NOUN dti-1354 204 8 vk vk PROPN dti-1354 204 9 , , PUNCT dti-1354 204 10 et et PROPN dti-1354 204 11 al al PROPN dti-1354 204 12 . . PROPN dti-1354 204 13 atovaquone atovaquone PROPN dti-1354 204 14 and and CCONJ dti-1354 204 15 azithromycin azithromycin PROPN dti-1354 204 16 for for ADP dti-1354 204 17 the the DET dti-1354 204 18 treatment treatment NOUN dti-1354 204 19 of of ADP dti-1354 204 20 babesiosis babesiosis NOUN dti-1354 204 21 . . PUNCT dti-1354 205 1 n n X dti-1354 205 2 engl engl PROPN dti-1354 205 3 j j PROPN dti-1354 205 4 med med PROPN dti-1354 205 5 . . PUNCT dti-1354 206 1 2000;343(20):1454–1458 2000;343(20):1454–1458 NUM dti-1354 206 2 . . PUNCT dti-1354 207 1 13 13 NUM dti-1354 207 2 . . X dti-1354 207 3 wittner wittner PROPN dti-1354 207 4 m m PROPN dti-1354 207 5 , , PUNCT dti-1354 207 6 lederman lederman PROPN dti-1354 207 7 j j PROPN dti-1354 207 8 , , PUNCT dti-1354 207 9 tanowitz tanowitz PROPN dti-1354 207 10 hb hb PROPN dti-1354 207 11 , , PUNCT dti-1354 207 12 rosenbaum rosenbaum PROPN dti-1354 207 13 gs gs PROPN dti-1354 207 14 , , PUNCT dti-1354 207 15 weiss weiss PROPN dti-1354 207 16 lm lm PROPN dti-1354 207 17 . . PUNCT dti-1354 207 18 atovaquone atovaquone PROPN dti-1354 207 19 in in ADP dti-1354 207 20 the the DET dti-1354 207 21 treatment treatment NOUN dti-1354 207 22 of of ADP dti-1354 207 23 babesia babesia NOUN dti-1354 207 24 microti microti PROPN dti-1354 207 25 infections infection NOUN dti-1354 207 26 in in ADP dti-1354 207 27 hamsters hamster NOUN dti-1354 207 28 . . PUNCT dti-1354 208 1 am be AUX dti-1354 208 2 j j PROPN dti-1354 208 3 trop trop X dti-1354 208 4 med med PROPN dti-1354 208 5 hyg hyg PROPN dti-1354 208 6 . . PROPN dti-1354 208 7 1996;55(2):219–222 1996;55(2):219–222 NUM dti-1354 208 8 . . PUNCT dti-1354 209 1 14 14 NUM dti-1354 209 2 . . PUNCT dti-1354 210 1 cornillot cornillot NOUN dti-1354 210 2 e e NOUN dti-1354 210 3 , , PUNCT dti-1354 210 4 hadj hadj NOUN dti-1354 210 5 - - PUNCT dti-1354 210 6 kaddour kaddour PROPN dti-1354 210 7 k k PROPN dti-1354 210 8 , , PUNCT dti-1354 210 9 dassouli dassouli PROPN dti-1354 210 10 a a X dti-1354 210 11 , , PUNCT dti-1354 210 12 et et PROPN dti-1354 210 13 al al PROPN dti-1354 210 14 . . PUNCT dti-1354 211 1 sequencing sequence VERB dti-1354 211 2 of of ADP dti-1354 211 3 the the DET dti-1354 211 4 smallest small ADJ dti-1354 211 5 apicomplexan apicomplexan NOUN dti-1354 211 6 genome genome NOUN dti-1354 211 7 from from ADP dti-1354 211 8 the the DET dti-1354 211 9 human human ADJ dti-1354 211 10 pathogen pathogen NOUN dti-1354 211 11 babesia babesia NOUN dti-1354 211 12 microti microti PROPN dti-1354 211 13 . . PUNCT dti-1354 212 1 nucleic nucleic ADJ dti-1354 212 2 acids acid NOUN dti-1354 212 3 res re NOUN dti-1354 212 4 . . PUNCT dti-1354 213 1 2012;40(18):9102–9114 2012;40(18):9102–9114 NUM dti-1354 213 2 . . PUNCT dti-1354 214 1 15 15 NUM dti-1354 214 2 . . PUNCT dti-1354 215 1 al al PROPN dti-1354 215 2 - - PUNCT dti-1354 215 3 anouti anouti PROPN dti-1354 215 4 f f NOUN dti-1354 215 5 , , PUNCT dti-1354 215 6 tomavo tomavo PROPN dti-1354 215 7 s s PROPN dti-1354 215 8 , , PUNCT dti-1354 215 9 parmley parmley PROPN dti-1354 215 10 s s PROPN dti-1354 215 11 , , PUNCT dti-1354 215 12 ananvoranich ananvoranich NOUN dti-1354 215 13 s. s. PROPN dti-1354 215 14 the the DET dti-1354 215 15 expression expression NOUN dti-1354 215 16 of of ADP dti-1354 215 17 lactate lactate NOUN dti-1354 215 18 dehydrogenase dehydrogenase NOUN dti-1354 215 19 is be AUX dti-1354 215 20 important important ADJ dti-1354 215 21 for for ADP dti-1354 215 22 the the DET dti-1354 215 23 cell cell NOUN dti-1354 215 24 cycle cycle NOUN dti-1354 215 25 of of ADP dti-1354 215 26 toxoplasma toxoplasma NOUN dti-1354 215 27 gondii gondii NOUN dti-1354 215 28 . . PUNCT dti-1354 216 1 j j PROPN dti-1354 216 2 biol biol PROPN dti-1354 216 3 chem chem NOUN dti-1354 216 4 . . PUNCT dti-1354 217 1 2004;279(50):52300–52311 2004;279(50):52300–52311 NUM dti-1354 217 2 . . PROPN dti-1354 218 1 16 16 NUM dti-1354 218 2 . . PUNCT dti-1354 219 1 kavanagh kavanagh PROPN dti-1354 219 2 kl kl PROPN dti-1354 219 3 , , PUNCT dti-1354 219 4 elling elling PROPN dti-1354 219 5 ra ra PROPN dti-1354 219 6 , , PUNCT dti-1354 219 7 wilson wilson PROPN dti-1354 219 8 dk dk PROPN dti-1354 219 9 . . PROPN dti-1354 219 10 structure structure NOUN dti-1354 219 11 of of ADP dti-1354 219 12 toxoplasma toxoplasma PROPN dti-1354 219 13 gondii gondii NOUN dti-1354 219 14 ldh1 ldh1 NOUN dti-1354 219 15 : : PUNCT dti-1354 219 16 active active ADJ dti-1354 219 17 - - PUNCT dti-1354 219 18 site site NOUN dti-1354 219 19 differences difference NOUN dti-1354 219 20 from from ADP dti-1354 219 21 human human ADJ dti-1354 219 22 lactate lactate NOUN dti-1354 219 23 dehydrogenases dehydrogenase NOUN dti-1354 219 24 and and CCONJ dti-1354 219 25 the the DET dti-1354 219 26 structural structural ADJ dti-1354 219 27 basis basis NOUN dti-1354 219 28 for for ADP dti-1354 219 29 efficient efficient ADJ dti-1354 219 30 apad+ apad+ ADP dti-1354 219 31 use use NOUN dti-1354 219 32 . . PUNCT dti-1354 220 1 biochemistry biochemistry NOUN dti-1354 220 2 . . PUNCT dti-1354 221 1 2004;43(4):879–889 2004;43(4):879–889 X dti-1354 221 2 . . PROPN dti-1354 221 3 17 17 NUM dti-1354 221 4 . . PUNCT dti-1354 222 1 gomez gomez PROPN dti-1354 222 2 ms ms PROPN dti-1354 222 3 , , PUNCT dti-1354 222 4 piper piper PROPN dti-1354 222 5 rc rc PROPN dti-1354 222 6 , , PUNCT dti-1354 222 7 hunsaker hunsaker PROPN dti-1354 222 8 la la PROPN dti-1354 222 9 , , PUNCT dti-1354 222 10 et et PROPN dti-1354 222 11 al al PROPN dti-1354 222 12 . . PROPN dti-1354 222 13 substrate substrate NOUN dti-1354 222 14 and and CCONJ dti-1354 222 15 cofactor cofactor NOUN dti-1354 222 16 specificity specificity NOUN dti-1354 222 17 and and CCONJ dti-1354 222 18 selective selective ADJ dti-1354 222 19 inhibition inhibition NOUN dti-1354 222 20 of of ADP dti-1354 222 21 lactate lactate NOUN dti-1354 222 22 dehydrogenase dehydrogenase NOUN dti-1354 222 23 from from ADP dti-1354 222 24 the the DET dti-1354 222 25 malarial malarial ADJ dti-1354 222 26 parasite parasite NOUN dti-1354 222 27 p. p. NOUN dti-1354 222 28 falciparum falciparum NOUN dti-1354 222 29 . . PUNCT dti-1354 223 1 mol mol ADP dti-1354 223 2 biochem biochem PROPN dti-1354 223 3 parasitol parasitol NOUN dti-1354 223 4 . . PUNCT dti-1354 224 1 1997;90(1):235–246 1997;90(1):235–246 NUM dti-1354 224 2 . . PROPN dti-1354 224 3 18 18 NUM dti-1354 224 4 . . PUNCT dti-1354 225 1 penna penna PROPN dti-1354 225 2 - - PUNCT dti-1354 225 3 coutinho coutinho PROPN dti-1354 225 4 j j PROPN dti-1354 225 5 , , PUNCT dti-1354 225 6 cortopassi cortopassi PROPN dti-1354 225 7 wa wa PROPN dti-1354 225 8 , , PUNCT dti-1354 225 9 oliveira oliveira PROPN dti-1354 225 10 aa aa PROPN dti-1354 225 11 , , PUNCT dti-1354 225 12 frança frança PROPN dti-1354 225 13 tc tc PROPN dti-1354 225 14 , , PUNCT dti-1354 225 15 krettli krettli PROPN dti-1354 225 16 au au PROPN dti-1354 225 17 . . PUNCT dti-1354 225 18 antimalarial antimalarial ADJ dti-1354 225 19 activity activity NOUN dti-1354 225 20 of of ADP dti-1354 225 21 potential potential ADJ dti-1354 225 22 inhibitors inhibitor NOUN dti-1354 225 23 of of ADP dti-1354 225 24 plasmodium plasmodium NOUN dti-1354 225 25 falciparum falciparum ADJ dti-1354 225 26 lactate lactate NOUN dti-1354 225 27 dehydrogenase dehydrogenase NOUN dti-1354 225 28 enzyme enzyme NOUN dti-1354 225 29 selected select VERB dti-1354 225 30 by by ADP dti-1354 225 31 docking dock VERB dti-1354 225 32 studies study NOUN dti-1354 225 33 . . PUNCT dti-1354 226 1 plos plos PROPN dti-1354 226 2 one one NUM dti-1354 226 3 . . PUNCT dti-1354 227 1 2011;6(7):e21237 2011;6(7):e21237 NUM dti-1354 227 2 . . PUNCT dti-1354 228 1 19 19 NUM dti-1354 228 2 . . X dti-1354 229 1 bork bork PROPN dti-1354 229 2 s s PROPN dti-1354 229 3 , , PUNCT dti-1354 229 4 okamura okamura PRON dti-1354 229 5 m m PROPN dti-1354 229 6 , , PUNCT dti-1354 229 7 boonchit boonchit VERB dti-1354 229 8 s s PROPN dti-1354 229 9 , , PUNCT dti-1354 229 10 hirata hirata ADJ dti-1354 229 11 h h NOUN dti-1354 229 12 , , PUNCT dti-1354 229 13 yokoyama yokoyama PROPN dti-1354 229 14 n n CCONJ dti-1354 229 15 , , PUNCT dti-1354 229 16 igarashi igarashi PROPN dti-1354 229 17 i. i. NOUN dti-1354 229 18 identification identification NOUN dti-1354 229 19 of of ADP dti-1354 229 20 babesia babesia NOUN dti-1354 229 21 bovis bovi VERB dti-1354 229 22 l l ADJ dti-1354 229 23 - - PUNCT dti-1354 229 24 lactate lactate NOUN dti-1354 229 25 dehydrogenase dehydrogenase NOUN dti-1354 229 26 as as ADP dti-1354 229 27 a a DET dti-1354 229 28 potential potential ADJ dti-1354 229 29 chemotherapeutical chemotherapeutical ADJ dti-1354 229 30 target target NOUN dti-1354 229 31 against against ADP dti-1354 229 32 bovine bovine NOUN dti-1354 229 33 babesiosis babesiosis NOUN dti-1354 229 34 . . PUNCT dti-1354 230 1 mol mol ADP dti-1354 230 2 biochem biochem PROPN dti-1354 230 3 parasitol parasitol NOUN dti-1354 230 4 . . PUNCT dti-1354 231 1 2004;136(2):165 2004;136(2):165 NUM dti-1354 231 2 . . NOUN dti-1354 232 1 20 20 NUM dti-1354 232 2 . . PUNCT dti-1354 233 1 granchi granchi PROPN dti-1354 233 2 c c PROPN dti-1354 233 3 , , PUNCT dti-1354 233 4 roy roy PROPN dti-1354 233 5 s s PROPN dti-1354 233 6 , , PUNCT dti-1354 233 7 giacomelli giacomelli PROPN dti-1354 233 8 c c AUX dti-1354 233 9 , , PUNCT dti-1354 233 10 et et PROPN dti-1354 233 11 al al PROPN dti-1354 233 12 . . PROPN dti-1354 233 13 discovery discovery PROPN dti-1354 233 14 of of ADP dti-1354 233 15 n n CCONJ dti-1354 233 16 - - PUNCT dti-1354 233 17 hydroxyindole hydroxyindole NOUN dti-1354 233 18 - - PUNCT dti-1354 233 19 based base VERB dti-1354 233 20 inhibitors inhibitor NOUN dti-1354 233 21 of of ADP dti-1354 233 22 human human ADJ dti-1354 233 23 lactate lactate NOUN dti-1354 233 24 dehydrogenase dehydrogenase NOUN dti-1354 233 25 isoform isoform NOUN dti-1354 233 26 a a DET dti-1354 233 27 ( ( PUNCT dti-1354 233 28 ldh ldh NOUN dti-1354 233 29 - - PUNCT dti-1354 233 30 a a NOUN dti-1354 233 31 ) ) PUNCT dti-1354 233 32 as as ADP dti-1354 233 33 starvation starvation NOUN dti-1354 233 34 agents agent NOUN dti-1354 233 35 against against ADP dti-1354 233 36 cancer cancer NOUN dti-1354 233 37 cells cell NOUN dti-1354 233 38 . . PUNCT dti-1354 234 1 j j PROPN dti-1354 234 2 med med PROPN dti-1354 234 3 chem chem NOUN dti-1354 234 4 . . PUNCT dti-1354 235 1 2011;54(6):1599–1612 2011;54(6):1599–1612 NOUN dti-1354 235 2 . . PUNCT dti-1354 236 1 21 21 NUM dti-1354 236 2 . . PUNCT dti-1354 237 1 luo luo PROPN dti-1354 237 2 y y PROPN dti-1354 237 3 , , PUNCT dti-1354 237 4 jia jia PROPN dti-1354 237 5 h h PROPN dti-1354 237 6 , , PUNCT dti-1354 237 7 terkawi terkawi PROPN dti-1354 237 8 ma ma PROPN dti-1354 237 9 , , PUNCT dti-1354 237 10 et et PROPN dti-1354 237 11 al al PROPN dti-1354 237 12 . . PUNCT dti-1354 237 13 identification identification NOUN dti-1354 237 14 and and CCONJ dti-1354 237 15 characterization characterization NOUN dti-1354 237 16 of of ADP dti-1354 237 17 a a DET dti-1354 237 18 novel novel NOUN dti-1354 237 19 secreted secrete VERB dti-1354 237 20 antigen antigen ADV dti-1354 237 21 1 1 NUM dti-1354 237 22 of of ADP dti-1354 237 23 babesia babesia NOUN dti-1354 237 24 microti microti NOUN dti-1354 237 25 and and CCONJ dti-1354 237 26 evaluation evaluation NOUN dti-1354 237 27 of of ADP dti-1354 237 28 its its PRON dti-1354 237 29 potential potential ADJ dti-1354 237 30 use use NOUN dti-1354 237 31 in in ADP dti-1354 237 32 enzyme enzyme NOUN dti-1354 237 33 - - PUNCT dti-1354 237 34 linked link VERB dti-1354 237 35 immunosorbent immunosorbent ADJ dti-1354 237 36 assay assay NOUN dti-1354 237 37 and and CCONJ dti-1354 237 38 immunochromatographic immunochromatographic ADJ dti-1354 237 39 test test NOUN dti-1354 237 40 . . PUNCT dti-1354 238 1 parasitol parasitol NOUN dti-1354 238 2 . . PUNCT dti-1354 239 1 2011;60(2):119–125 2011;60(2):119–125 X dti-1354 239 2 . . X dti-1354 240 1 22 22 NUM dti-1354 240 2 . . PUNCT dti-1354 241 1 cao cao PROPN dti-1354 241 2 s s PROPN dti-1354 241 3 , , PUNCT dti-1354 241 4 luo luo PROPN dti-1354 241 5 y y PROPN dti-1354 241 6 , , PUNCT dti-1354 241 7 aboge aboge PROPN dti-1354 241 8 go go VERB dti-1354 241 9 , , PUNCT dti-1354 241 10 et et PROPN dti-1354 241 11 al al PROPN dti-1354 241 12 . . PUNCT dti-1354 241 13 identification identification NOUN dti-1354 241 14 and and CCONJ dti-1354 241 15 characterization characterization NOUN dti-1354 241 16 of of ADP dti-1354 241 17 an an DET dti-1354 241 18 interspersed interspersed ADJ dti-1354 241 19 repeat repeat NOUN dti-1354 241 20 antigen antigen NOUN dti-1354 241 21 of of ADP dti-1354 241 22 babesia babesia NOUN dti-1354 241 23 microti microti PROPN dti-1354 241 24 ( ( PUNCT dti-1354 241 25 bmira bmira PROPN dti-1354 241 26 ) ) PUNCT dti-1354 241 27 . . PUNCT dti-1354 242 1 exp exp NOUN dti-1354 242 2 parasitol parasitol NOUN dti-1354 242 3 . . PUNCT dti-1354 243 1 2013;133(3 2013;133(3 NUM dti-1354 243 2 ): ): PUNCT dti-1354 244 1 346–352 346–352 NUM dti-1354 244 2 . . PUNCT dti-1354 245 1 23 23 NUM dti-1354 245 2 . . PUNCT dti-1354 246 1 luo luo PROPN dti-1354 246 2 y y PROPN dti-1354 246 3 , , PUNCT dti-1354 246 4 terkawi terkawi PROPN dti-1354 246 5 ma ma PROPN dti-1354 246 6 , , PUNCT dti-1354 246 7 jia jia PROPN dti-1354 246 8 h h PROPN dti-1354 246 9 , , PUNCT dti-1354 246 10 et et PROPN dti-1354 246 11 al al PROPN dti-1354 246 12 . . PUNCT dti-1354 247 1 a a DET dti-1354 247 2 double double ADJ dti-1354 247 3 antibody antibody NOUN dti-1354 247 4 sandwich sandwich NOUN dti-1354 247 5 enzyme enzyme NOUN dti-1354 247 6 - - PUNCT dti-1354 247 7 linked link VERB dti-1354 247 8 immunosorbent immunosorbent NOUN dti-1354 247 9 assay assay NOUN dti-1354 247 10 for for ADP dti-1354 247 11 detection detection NOUN dti-1354 247 12 of of ADP dti-1354 247 13 secreted secrete VERB dti-1354 247 14 antigen antigen ADV dti-1354 247 15 1 1 NUM dti-1354 247 16 of of ADP dti-1354 247 17 babesia babesia NOUN dti-1354 247 18 microti microti PROPN dti-1354 247 19 using use VERB dti-1354 247 20 hamster hamster PROPN dti-1354 247 21 model model NOUN dti-1354 247 22 . . PUNCT dti-1354 248 1 exp exp NOUN dti-1354 248 2 parasitol parasitol NOUN dti-1354 248 3 . . PUNCT dti-1354 249 1 2012;130(2):178–182 2012;130(2):178–182 NUM dti-1354 249 2 . . PUNCT dti-1354 250 1 24 24 NUM dti-1354 250 2 . . PUNCT dti-1354 250 3 terkawi terkawi PROPN dti-1354 250 4 ma ma PROPN dti-1354 250 5 , , PUNCT dti-1354 250 6 aboge aboge PROPN dti-1354 250 7 g g PROPN dti-1354 250 8 , , PUNCT dti-1354 250 9 jia jia PROPN dti-1354 250 10 h h PROPN dti-1354 250 11 , , PUNCT dti-1354 250 12 et et PROPN dti-1354 250 13 al al PROPN dti-1354 250 14 . . PROPN dti-1354 251 1 molecular molecular ADJ dti-1354 251 2 and and CCONJ dti-1354 251 3 immunological immunological ADJ dti-1354 251 4 characterization characterization NOUN dti-1354 251 5 of of ADP dti-1354 251 6 babesia babesia NOUN dti-1354 251 7 gibsoni gibsoni ADJ dti-1354 251 8 and and CCONJ dti-1354 251 9 babesia babesia PROPN dti-1354 251 10 microti microti PROPN dti-1354 251 11 heat heat NOUN dti-1354 251 12 shock shock NOUN dti-1354 251 13 protein-70 protein-70 PROPN dti-1354 251 14 . . PUNCT dti-1354 252 1 parasite parasite ADJ dti-1354 252 2 immunol immunol NOUN dti-1354 252 3 . . PUNCT dti-1354 253 1 2009;31(6):328–340 2009;31(6):328–340 NUM dti-1354 253 2 . . X dti-1354 253 3 25 25 NUM dti-1354 253 4 . . X dti-1354 253 5 de de PROPN dti-1354 253 6 la la PROPN dti-1354 253 7 casa casa PROPN dti-1354 253 8 - - PROPN dti-1354 253 9 esperón esperón PROPN dti-1354 253 10 e. e. PROPN dti-1354 253 11 horizontal horizontal PROPN dti-1354 253 12 transfer transfer NOUN dti-1354 253 13 and and CCONJ dti-1354 253 14 the the DET dti-1354 253 15 evolution evolution NOUN dti-1354 253 16 of of ADP dti-1354 253 17 host host NOUN dti-1354 253 18 - - PUNCT dti-1354 253 19 pathogen pathogen NOUN dti-1354 253 20 interactions interaction NOUN dti-1354 253 21 . . PUNCT dti-1354 254 1 int int PROPN dti-1354 254 2 j j PROPN dti-1354 254 3 evol evol NOUN dti-1354 254 4 biol biol PROPN dti-1354 254 5 . . PUNCT dti-1354 255 1 2012;2012:9 2012;2012:9 NUM dti-1354 255 2 . . PUNCT dti-1354 256 1 26 26 NUM dti-1354 256 2 . . PUNCT dti-1354 257 1 yhu yhu PROPN dti-1354 257 2 s s PROPN dti-1354 257 3 - - PROPN dti-1354 257 4 k k ADJ dti-1354 257 5 , , PUNCT dti-1354 257 6 park park NOUN dti-1354 257 7 h h PROPN dti-1354 257 8 - - PUNCT dti-1354 257 9 s s PROPN dti-1354 257 10 , , PUNCT dti-1354 257 11 kim kim PROPN dti-1354 257 12 h h PROPN dti-1354 257 13 - - PUNCT dti-1354 257 14 y y PROPN dti-1354 257 15 , , PUNCT dti-1354 257 16 et et PROPN dti-1354 257 17 al al PROPN dti-1354 257 18 . . PUNCT dti-1354 257 19 expression expression NOUN dti-1354 257 20 of of ADP dti-1354 257 21 lactate lactate NOUN dti-1354 257 22 dehydrogenase dehydrogenase NOUN dti-1354 257 23 a a DET dti-1354 257 24 - - PUNCT dti-1354 257 25 gene gene NOUN dti-1354 257 26 in in ADP dti-1354 257 27 regenerating regenerate VERB dti-1354 257 28 rat rat NOUN dti-1354 257 29 liver liver NOUN dti-1354 257 30 . . PUNCT dti-1354 258 1 arch arch ADJ dti-1354 258 2 pharm pharm NOUN dti-1354 258 3 res re NOUN dti-1354 258 4 . . PUNCT dti-1354 259 1 1988;11(4):278–284 1988;11(4):278–284 NUM dti-1354 259 2 . . PUNCT dti-1354 260 1 27 27 NUM dti-1354 260 2 . . PUNCT dti-1354 261 1 ronai ronai VERB dti-1354 261 2 z. z. PROPN dti-1354 261 3 glycolytic glycolytic ADJ dti-1354 261 4 enzymes enzyme NOUN dti-1354 261 5 as as ADP dti-1354 261 6 dna dna NOUN dti-1354 261 7 binding bind VERB dti-1354 261 8 proteins protein NOUN dti-1354 261 9 . . PUNCT dti-1354 262 1 int int PROPN dti-1354 262 2 j j PROPN dti-1354 262 3 biochem biochem PROPN dti-1354 262 4 . . PUNCT dti-1354 263 1 1993;25(7 1993;25(7 NUM dti-1354 263 2 ): ): PUNCT dti-1354 263 3 1073–1076 1073–1076 NUM dti-1354 263 4 . . PUNCT dti-1354 264 1 28 28 NUM dti-1354 264 2 . . PUNCT dti-1354 265 1 shoemark shoemark PROPN dti-1354 265 2 dk dk PROPN dti-1354 265 3 , , PUNCT dti-1354 265 4 cliff cliff PROPN dti-1354 265 5 mj mj PROPN dti-1354 265 6 , , PUNCT dti-1354 265 7 sessions session NOUN dti-1354 265 8 rb rb NOUN dti-1354 265 9 , , PUNCT dti-1354 265 10 clarke clarke PROPN dti-1354 265 11 ar ar PROPN dti-1354 265 12 . . PROPN dti-1354 266 1 enzymatic enzymatic ADJ dti-1354 266 2 properties property NOUN dti-1354 266 3 of of ADP dti-1354 266 4 the the DET dti-1354 266 5 lactate lactate NOUN dti-1354 266 6 dehydrogenase dehydrogenase NOUN dti-1354 266 7 enzyme enzyme NOUN dti-1354 266 8 from from ADP dti-1354 266 9 plasmodium plasmodium NOUN dti-1354 266 10 falciparum falciparum NOUN dti-1354 266 11 . . PUNCT dti-1354 267 1 febs febs PROPN dti-1354 267 2 j. j. PROPN dti-1354 267 3 2007;274(11):2738–2748 2007;274(11):2738–2748 PROPN dti-1354 267 4 . . PROPN dti-1354 267 5 29 29 NUM dti-1354 267 6 . . PUNCT dti-1354 268 1 dando dando PROPN dti-1354 268 2 c c PROPN dti-1354 268 3 , , PUNCT dti-1354 268 4 schroeder schroeder PROPN dti-1354 268 5 er er INTJ dti-1354 268 6 , , PUNCT dti-1354 268 7 hunsaker hunsaker PROPN dti-1354 268 8 la la PROPN dti-1354 268 9 , , PUNCT dti-1354 268 10 et et PROPN dti-1354 268 11 al al PROPN dti-1354 268 12 . . PUNCT dti-1354 269 1 the the DET dti-1354 269 2 kinetic kinetic ADJ dti-1354 269 3 properties property NOUN dti-1354 269 4 and and CCONJ dti-1354 269 5 sensitivities sensitivity NOUN dti-1354 269 6 to to ADP dti-1354 269 7 inhibitors inhibitor NOUN dti-1354 269 8 of of ADP dti-1354 269 9 lactate lactate NOUN dti-1354 269 10 dehydrogenases dehydrogenase NOUN dti-1354 269 11 ( ( PUNCT dti-1354 269 12 ldh1 ldh1 PROPN dti-1354 269 13 and and CCONJ dti-1354 269 14 ldh2 ldh2 PROPN dti-1354 269 15 ) ) PUNCT dti-1354 269 16 from from ADP dti-1354 269 17 toxoplasma toxoplasma ADJ dti-1354 269 18 gondii gondii NOUN dti-1354 269 19 : : PUNCT dti-1354 269 20 comparisons comparison NOUN dti-1354 269 21 with with ADP dti-1354 269 22 pldh pldh ADJ dti-1354 269 23 from from ADP dti-1354 269 24 plasmodium plasmodium NOUN dti-1354 269 25 falciparum falciparum NOUN dti-1354 269 26 . . PUNCT dti-1354 270 1 mol mol ADP dti-1354 270 2 biochem biochem PROPN dti-1354 270 3 parasitol parasitol NOUN dti-1354 270 4 . . PUNCT dti-1354 271 1 2001;118(1):23–32 2001;118(1):23–32 X dti-1354 271 2 . . PUNCT dti-1354 272 1 30 30 NUM dti-1354 272 2 . . PUNCT dti-1354 273 1 hewitt hewitt PROPN dti-1354 273 2 co co PROPN dti-1354 273 3 , , PUNCT dti-1354 273 4 eszes esze NOUN dti-1354 273 5 cm cm NOUN dti-1354 273 6 , , PUNCT dti-1354 273 7 sessions session NOUN dti-1354 273 8 rb rb VERB dti-1354 273 9 , , PUNCT dti-1354 273 10 et et PROPN dti-1354 273 11 al al PROPN dti-1354 273 12 . . PUNCT dti-1354 274 1 a a DET dti-1354 274 2 general general ADJ dti-1354 274 3 method method NOUN dti-1354 274 4 for for ADP dti-1354 274 5 relieving relieve VERB dti-1354 274 6 substrate substrate NOUN dti-1354 274 7 inhibition inhibition NOUN dti-1354 274 8 in in ADP dti-1354 274 9 lactate lactate NOUN dti-1354 274 10 dehydrogenases dehydrogenase NOUN dti-1354 274 11 . . PUNCT dti-1354 275 1 protein protein NOUN dti-1354 275 2 eng eng PROPN dti-1354 275 3 . . PROPN dti-1354 275 4 1999;12(6):491–496 1999;12(6):491–496 PROPN dti-1354 275 5 . . PROPN dti-1354 275 6 31 31 NUM dti-1354 275 7 . . PUNCT dti-1354 276 1 xu xu PROPN dti-1354 277 1 l l PROPN dti-1354 277 2 , , PUNCT dti-1354 277 3 yang yang PROPN dti-1354 277 4 d d PROPN dti-1354 277 5 , , PUNCT dti-1354 277 6 wang wang PROPN dti-1354 277 7 s s PROPN dti-1354 277 8 , , PUNCT dti-1354 277 9 et et PROPN dti-1354 277 10 al al PROPN dti-1354 277 11 . . PUNCT dti-1354 278 1 ( ( PUNCT dti-1354 278 2 -)-gossypol -)-gossypol NOUN dti-1354 278 3 enhances enhance VERB dti-1354 278 4 response response NOUN dti-1354 278 5 to to ADP dti-1354 278 6 radiation radiation NOUN dti-1354 278 7 therapy therapy NOUN dti-1354 278 8 and and CCONJ dti-1354 278 9 results result NOUN dti-1354 278 10 in in ADP dti-1354 278 11 tumor tumor NOUN dti-1354 278 12 regression regression NOUN dti-1354 278 13 of of ADP dti-1354 278 14 human human ADJ dti-1354 278 15 prostate prostate NOUN dti-1354 278 16 cancer cancer NOUN dti-1354 278 17 . . PUNCT dti-1354 279 1 mol mol ADP dti-1354 279 2 cancer cancer NOUN dti-1354 279 3 ther ther ADV dti-1354 279 4 . . PUNCT dti-1354 280 1 2005;4(2):197–205 2005;4(2):197–205 X dti-1354 280 2 . . NOUN dti-1354 280 3 32 32 NUM dti-1354 280 4 . . PUNCT dti-1354 281 1 beutler beutler PROPN dti-1354 281 2 e. e. PROPN dti-1354 281 3 energy energy PROPN dti-1354 281 4 metabolism metabolism NOUN dti-1354 281 5 and and CCONJ dti-1354 281 6 maintenance maintenance NOUN dti-1354 281 7 of of ADP dti-1354 281 8 erythrocytes erythrocyte NOUN dti-1354 281 9 . . PUNCT dti-1354 282 1 williams williams PROPN dti-1354 282 2 hematology hematology PROPN dti-1354 282 3 . . PUNCT dti-1354 283 1 6th 6th ADJ dti-1354 283 2 ed ed NOUN dti-1354 283 3 . . PUNCT dti-1354 283 4 lichtman lichtman PROPN dti-1354 283 5 am be AUX dti-1354 283 6 , , PUNCT dti-1354 283 7 editor editor NOUN dti-1354 283 8 new new PROPN dti-1354 283 9 york york PROPN dti-1354 283 10 : : PUNCT dti-1354 283 11 mcgraw mcgraw PROPN dti-1354 283 12 - - PUNCT dti-1354 283 13 hill hill NOUN dti-1354 283 14 ; ; PUNCT dti-1354 284 1 2001:319–332 2001:319–332 X dti-1354 284 2 . . PUNCT dti-1354 285 1 33 33 NUM dti-1354 285 2 . . PUNCT dti-1354 286 1 merkle merkle NOUN dti-1354 286 2 s s PART dti-1354 286 3 , , PUNCT dti-1354 286 4 favor favor VERB dti-1354 286 5 j j PROPN dti-1354 286 6 , , PUNCT dti-1354 286 7 graw graw PROPN dti-1354 286 8 j j PROPN dti-1354 286 9 , , PUNCT dti-1354 286 10 hornhardt hornhardt PROPN dti-1354 286 11 s s NOUN dti-1354 286 12 , , PUNCT dti-1354 286 13 pretsch pretsch PROPN dti-1354 286 14 w. w. PROPN dti-1354 286 15 hereditary hereditary PROPN dti-1354 286 16 lactate lactate PROPN dti-1354 286 17 dehydrogenase dehydrogenase VERB dti-1354 286 18 a a DET dti-1354 286 19 subunit subunit NOUN dti-1354 286 20 deficiency deficiency NOUN dti-1354 286 21 as as ADP dti-1354 286 22 cause cause NOUN dti-1354 286 23 of of ADP dti-1354 286 24 early early ADJ dti-1354 286 25 postimplantation postimplantation NOUN dti-1354 286 26 death death NOUN dti-1354 286 27 of of ADP dti-1354 286 28 homozygotes homozygote NOUN dti-1354 286 29 in in ADP dti-1354 286 30 mus mus PROPN dti-1354 286 31 musculus musculus NOUN dti-1354 286 32 . . PUNCT dti-1354 287 1 genetics genetic NOUN dti-1354 287 2 . . PUNCT dti-1354 288 1 1992;131(2):413–421 1992;131(2):413–421 NUM dti-1354 288 2 . . NOUN dti-1354 288 3 34 34 NUM dti-1354 288 4 . . PUNCT dti-1354 288 5 pelicano pelicano PROPN dti-1354 288 6 h h PROPN dti-1354 288 7 , , PUNCT dti-1354 288 8 martin martin PROPN dti-1354 288 9 ds ds PROPN dti-1354 288 10 , , PUNCT dti-1354 288 11 xu xu PROPN dti-1354 288 12 rh rh PROPN dti-1354 288 13 , , PUNCT dti-1354 288 14 huang huang PROPN dti-1354 288 15 p. p. PROPN dti-1354 288 16 glycolysis glycolysis NOUN dti-1354 288 17 inhibition inhibition NOUN dti-1354 288 18 for for ADP dti-1354 288 19 anticancer anticancer NOUN dti-1354 288 20 treatment treatment NOUN dti-1354 288 21 . . PUNCT dti-1354 289 1 oncogene oncogene NOUN dti-1354 289 2 . . PUNCT dti-1354 290 1 2006;25(34):4633–4646 2006;25(34):4633–4646 PROPN dti-1354 290 2 . . PUNCT dti-1354 291 1 35 35 NUM dti-1354 291 2 . . PUNCT dti-1354 292 1 zhou zhou PROPN dti-1354 292 2 m m PROPN dti-1354 292 3 , , PUNCT dti-1354 292 4 zhao zhao PROPN dti-1354 292 5 y y PROPN dti-1354 292 6 , , PUNCT dti-1354 292 7 ding de VERB dti-1354 292 8 y y PROPN dti-1354 292 9 , , PUNCT dti-1354 292 10 et et PROPN dti-1354 292 11 al al PROPN dti-1354 292 12 . . PROPN dti-1354 292 13 warburg warburg PROPN dti-1354 292 14 effect effect NOUN dti-1354 292 15 in in ADP dti-1354 292 16 chemosensitivity chemosensitivity NOUN dti-1354 292 17 : : PUNCT dti-1354 292 18 targeting target VERB dti-1354 292 19 lactate lactate NOUN dti-1354 292 20 dehydrogenase dehydrogenase NOUN dti-1354 292 21 - - PUNCT dti-1354 292 22 a a PRON dti-1354 292 23 re re NOUN dti-1354 292 24 - - NOUN dti-1354 292 25 sensitizes sensitize VERB dti-1354 292 26 taxol taxol NOUN dti-1354 292 27 - - PUNCT dti-1354 292 28 resistant resistant ADJ dti-1354 292 29 cancer cancer NOUN dti-1354 292 30 cells cell NOUN dti-1354 292 31 to to ADP dti-1354 292 32 taxol taxol PROPN dti-1354 292 33 . . PUNCT dti-1354 293 1 mol mol ADP dti-1354 293 2 cancer cancer NOUN dti-1354 293 3 . . PUNCT dti-1354 294 1 2010;9:33 2010;9:33 NOUN dti-1354 294 2 . . PUNCT dti-1354 295 1 http://www.la-press.com http://www.la-press.com X dti-1354 296 1 vudriko vudriko ADV dti-1354 296 2 et et PROPN dti-1354 296 3 al al PROPN dti-1354 296 4 38 38 NUM dti-1354 296 5 drug drug NOUN dti-1354 296 6 target target NOUN dti-1354 296 7 insights insight VERB dti-1354 296 8 2014:8 2014:8 NUM dti-1354 296 9 supplementary supplementary ADJ dti-1354 296 10 data datum NOUN dti-1354 296 11 supplementary supplementary ADJ dti-1354 296 12 table table NOUN dti-1354 296 13 1 1 NUM dti-1354 296 14 . . PUNCT dti-1354 296 15 unique unique ADJ dti-1354 296 16 amino amino NOUN dti-1354 296 17 acid acid NOUN dti-1354 296 18 residues residue NOUN dti-1354 296 19 at at ADP dti-1354 296 20 antigenic antigenic ADJ dti-1354 296 21 domain domain NOUN dti-1354 296 22 of of ADP dti-1354 296 23 bmldh bmldh PROPN dti-1354 296 24 compared compare VERB dti-1354 296 25 to to ADP dti-1354 296 26 other other ADJ dti-1354 296 27 apicomplexan apicomplexan NOUN dti-1354 296 28 parasites parasite NOUN dti-1354 296 29 and and CCONJ dti-1354 296 30 mammalian mammalian PROPN dti-1354 296 31 ldh ldh PROPN dti-1354 296 32 . . PUNCT dti-1354 297 1 ldh ldh PROPN dti-1354 297 2 for for ADP dti-1354 297 3 different different ADJ dti-1354 297 4 species specie NOUN dti-1354 297 5 unique unique ADJ dti-1354 297 6 antigenic antigenic ADJ dti-1354 297 7 bmldh bmldh ADJ dti-1354 297 8 amino amino ADJ dti-1354 297 9 acids acid NOUN dti-1354 297 10 residues residue NOUN dti-1354 297 11 at at ADP dti-1354 297 12 specific specific ADJ dti-1354 297 13 sequence sequence NOUN dti-1354 297 14 numbers number NOUN dti-1354 297 15 compared compare VERB dti-1354 297 16 to to ADP dti-1354 297 17 other other ADJ dti-1354 297 18 ldh ldh PROPN dti-1354 297 19 10 10 NUM dti-1354 297 20 42 42 NUM dti-1354 297 21 52 52 NUM dti-1354 297 22 191 191 NUM dti-1354 297 23 211 211 NUM dti-1354 297 24 228 228 NUM dti-1354 297 25 311 311 NUM dti-1354 297 26 327 327 NUM dti-1354 297 27 b. b. PROPN dti-1354 297 28 microti microti PROPN dti-1354 297 29 gray gray PROPN dti-1354 297 30 strain strain NOUN dti-1354 297 31 ldh ldh PROPN dti-1354 297 32 l l PROPN dti-1354 297 33 n n CCONJ dti-1354 297 34 i i PRON dti-1354 297 35 n n VERB dti-1354 297 36 l l NOUN dti-1354 297 37 v v X dti-1354 297 38 e e NOUN dti-1354 297 39 n n PRON dti-1354 297 40 homo homo NOUN dti-1354 297 41 sapien sapien PROPN dti-1354 297 42 ldh ldh VERB dti-1354 298 1 i i PROPN dti-1354 298 2 l l NOUN dti-1354 299 1 v v NOUN dti-1354 299 2 s s X dti-1354 299 3 v v NOUN dti-1354 299 4 d d X dti-1354 299 5 d d X dti-1354 299 6 k k PROPN dti-1354 299 7 mus mus PROPN dti-1354 299 8 musculus musculus PROPN dti-1354 299 9 ldh ldh PROPN dti-1354 300 1 i i PRON dti-1354 300 2 m m VERB dti-1354 300 3 v v ADP dti-1354 300 4 s s X dti-1354 300 5 v v NOUN dti-1354 300 6 d d X dti-1354 300 7 d d X dti-1354 300 8 k k PROPN dti-1354 300 9 cricetulus cricetulus PROPN dti-1354 300 10 griseus griseus PROPN dti-1354 300 11 ldh ldh PROPN dti-1354 301 1 i i PRON dti-1354 301 2 m m VERB dti-1354 301 3 v v ADP dti-1354 301 4 s s X dti-1354 301 5 v v NOUN dti-1354 301 6 d d X dti-1354 301 7 d d X dti-1354 301 8 k k PROPN dti-1354 301 9 p. p. NOUN dti-1354 301 10 facuparum facuparum NOUN dti-1354 302 1 ldh ldh VERB dti-1354 302 2 n n CCONJ dti-1354 302 3 l l NOUN dti-1354 302 4 f f PROPN dti-1354 303 1 d d NOUN dti-1354 303 2 i i PRON dti-1354 303 3 l l NOUN dti-1354 303 4 q q X dti-1354 303 5 e e PROPN dti-1354 303 6 b. b. PROPN dti-1354 303 7 bovis bovi VERB dti-1354 303 8 ldh ldh PROPN dti-1354 303 9 – – PUNCT dti-1354 303 10 l l NOUN dti-1354 303 11 f f X dti-1354 303 12 y y PROPN dti-1354 303 13 v v X dti-1354 303 14 e e X dti-1354 303 15 k k PROPN dti-1354 303 16 a a DET dti-1354 303 17 t. t. PROPN dti-1354 303 18 annulata annulata PROPN dti-1354 303 19 ldh ldh X dti-1354 303 20 – – PUNCT dti-1354 303 21 l l NOUN dti-1354 303 22 l l X dti-1354 303 23 y y PROPN dti-1354 303 24 s s X dti-1354 303 25 y y PROPN dti-1354 303 26 k k PROPN dti-1354 303 27 a a DET dti-1354 303 28 t. t. PROPN dti-1354 303 29 gondii gondii NOUN dti-1354 303 30 ldh ldh NOUN dti-1354 303 31 – – PUNCT dti-1354 303 32 l l NOUN dti-1354 304 1 f f PUNCT dti-1354 305 1 d d X dti-1354 305 2 v v X dti-1354 305 3 k k X dti-1354 305 4 q q X dti-1354 305 5 k k NOUN dti-1354 305 6 note note NOUN dti-1354 305 7 : : PUNCT dti-1354 305 8 the the DET dti-1354 305 9 antigenic antigenic ADJ dti-1354 305 10 amino amino NOUN dti-1354 305 11 acid acid NOUN dti-1354 305 12 domains domain NOUN dti-1354 305 13 for for ADP dti-1354 305 14 bmldh bmldh ADJ dti-1354 305 15 were be AUX dti-1354 305 16 elucidated elucidate VERB dti-1354 305 17 using use VERB dti-1354 305 18 jambw jambw PROPN dti-1354 305 19 antigenicity antigenicity PROPN dti-1354 305 20 plot plot NOUN dti-1354 305 21 followed follow VERB dti-1354 305 22 by by ADP dti-1354 305 23 multiple multiple ADJ dti-1354 305 24 sequence sequence NOUN dti-1354 305 25 alignment alignment NOUN dti-1354 305 26 to to PART dti-1354 305 27 determine determine VERB dti-1354 305 28 unique unique ADJ dti-1354 305 29 amino amino NOUN dti-1354 305 30 acid acid NOUN dti-1354 305 31 residues residue NOUN dti-1354 305 32 for for ADP dti-1354 305 33 bmldh bmldh ADJ dti-1354 305 34 at at ADP dti-1354 305 35 the the DET dti-1354 305 36 antigenic antigenic ADJ dti-1354 305 37 sites site NOUN dti-1354 305 38 . . PUNCT dti-1354 305 39 http://www.la-press.com http://www.la-press.com X