Microsoft Word - Suppl.+File+Lihle+2024+(2) Drug Target Insights 2024 | DOI: 10.33393/d .2024.3219 | Nxasana et al. Supplementary Tables SUPPL. TABLE I- Cell material used for the study Type of cell line ATCC generic name Disease Disease markers 3T3 ATCC1®CRL-1658™ Normal Rat muscle N/A HT-29 ATCC1® HTB-38™ Colorectal adenocarcinoma N/A HCT-15 ATCC® CCL-225™ Colorectal adenocarcinoma, dukes type C/ resistant variant P-gp N/A: Not Applicable, P-gp: P-glycoprotein SUPPL.TABLE II- Analysis for combination index range Range of combination index Description <0.1 Very strong synergism 0.1-0.3 Strong synergism 0.3-0.7 Synergism 0.7-0.85 Moderate synergism 0.85-0.90 Slight synergism 0.90-1.10 Nearly additive 1.10-1.20 Slight antagonism 1.20-1.45 Moderate antagonism 1.45-3.3 Antagonism 3.3-10 Strong antagonism >10 Very strong antagonism TABLE III- The retention factors of the separated components of the S. birrea ethanol extract using different mobile phases Mobile phases Compound Retention factor DCM: Hexane: Ethyl Acetate 1 0.47 2 0.65 3 0.93 4 0.99 Benzene: methanol: Ammonium hydroxide 1 0.2 2 0.35 3 0.54 4 0.88 5 0.95 Suppl. Table IV. The IC50 and combination index values of the DOX and VER on HT29 cells, n=3. Drug/Combo CI value Dose VER Dose DOX DRI VER DRI DOX IC50 VER 3.04 2.3 DOX 0.45 1.7 DOX + VER 1.00 1.31 0.26 IC75 3.5 Drug Target Insights 2024 | DOI: 10.33393/d .2024.3219 | Nxasana et al. VER 23.06 7.8 DOX 2.07 DOX + VER 0.41 2.94 0.59 IC90 VER 175.1 26 DOX 9.40 7 DOX + VER 0.17 6.61 1.32 IC95 VER 695.4 60 DOX 26.7 11.4 DOX+ VER 0.10 11.5 2.29 Suppl. Table V. The IC and combination index values of the DOX and SBE extract on HT29 cells, n=3. Drug/Combination CI value Dose-SBE Dose-DOX DRI-SBE DRI-DOX IC50 SBE 140.09 0.21 DOX 0.35 0.20 SBE + DOX 9.6 669.89 1.70 IC75 SBE 689.90 0.14 DOX 1.26 0.10 SBE + DOX 17.1 4969.46 12.6 IC90 SBE 3397.65 0.09 DOX 4.5 0.04 SBE + DOX 31.6 36865.0 93.6 IC95 SBE 10048.5 0.07 DOX 10.7 0.02 SBE + DOX 48.5 144061 365.9 Drug Target Insights 2024 | DOI: 10.33393/d .2024.3219 | Nxasana et al. Suppl. Table VI. The IC and combination index values of DOX and SBW extract on HT29 cells, n=3. Drug/Combination CI value Dose S.B H2O Dose DOX DRI SBW DRI DOX IC50 SBW 208.0 0.3 DOX 0.47 0.4 SBW + DOX 2.5 275.4 0.57 IC75 SBW 940.70 0.21 DOX 2.04 0.50 SBW + DOX 0.97 467.39 0.97 IC90 SBW 4253.9 0.13 DOX 8.89 0.59 SBW + DOX 0.37 793.13 1.64 IC95 SBW 11871.9 0.09 DOX 24.18 0.66 SBW + DOX 0.19 1136.5 2.36 Suppl. Table VII. The IC and combination index values of DOX and VER on HCT15 cells, n=3. Drug/Combination CI value Dose VER Dose DOX DRI VER DRI DOX IC50 VER 6.63 2.64 DOX 0.38 0.46 DOX + VER 1.33 2.99 0.33 IC75 VER 217.40 355.6 DOX 6.11 1.1 DOX + VER 0.34 14.82 1.66 IC90 VER 7127.23 47807.7 DOX 97.73 3.0 DOX + VER 0.09 73.44 8.25 IC95 VER 7652.5 1340243 DOX 643.6 4.2 DOX+ VER 0.04 218.07 24.50 Drug Target Insights 2024 | DOI: 10.33393/d .2024.3219 | Nxasana et al. Suppl. Table VIII. The IC and combination index values of DOX and SBE extract on HCT15 cells. Drug/Combination CI value Dose SB-EE Dose DOX DRI SB- EE DRI DOX IC50 SBE 49.35 0.54 DOX 0.68 0.51 SBE + DOX 3.75 89.86 1.32 IC75 SBE 256.47 7.37 DOX 4.12 8.05 SBE + DOX 0.26 34.86 0.51 IC90 SBE 1332.76 98.5 DOX 24.87 125.1 SBE + DOX 0.01 13.53 0.20 IC95 SBE 4088.34 578.37 DOX 84.43 809.18 SBE + DOX 0.00297 7.11 0.10 Suppl. Table IX. The IC and combination index values of DOX and SBW extract on HCT15 cells, n=3. Drug/Combination CI value Dose SBW Dose DOX DRI SBW DRI DOX IC50 SBW 413.41 0.23 DOX 1.54 0.23 SBW + DOX 5.30 1272.60 3.62 IC75 SBW 1689.40 0.15 DOX 11.33 0.18 SBW + DOX 6.66 7903.88 22.50 IC90 SBW 6615.65 0.07 DOX 83.28 0.14 SBW + DOX 10.00 49089.5 139.74 IC95 SBW 16741.3 0.05 DOX 323.40 0.11 SBW + DOX 11.65 169998 483.91 Drug Target Insights 2024 | DOI: 10.33393/d .2024.3219 | Nxasana et al. Supplementary Figures Suppl. Fig. 1 - TLC Chemical fingerprint of the SBE extract. (A) DCM: EA: HEX (B) Benzene: MeOH: NH3OH at 366 nm. (A) (B)