Microsoft Word - Supplementary+File Drug Target Insights 2025 | DOI: 10.33393/dti.2025.3304 | Kaushik et al Figure S1. CLAP-TB assay standarized in a water kettle (a) RT-LAMP reaction of IS6110 gene for MTB RNA and NTC visualized by colour change from pink to yellow after 40 min between 60-65°C in a water kettle (b) The agarose gel image of amplified product obtained from RT-LAMP reaction. (c) The lateral flow readout results of IS6110 gene for MTB and NTC after 3 min. Drug Target Insights 2025 | DOI: 10.33393/dti.2025.3304 | Kaushik et al Figure S2. CLAP-TB assay standarized in a thermos flask (a) RT-LAMP reaction of IS6110 gene for MTB RNA and NTC visualized by colour change from pink to yellow after 40 min between 60- 65°C in a thermos flask (b) The agarose gel image of amplified product obtained from RT-LAMP reaction. (c) The lateral flow readout results of IS6110 gene for MTB and NTC after 3 min. Drug Target Insights 2025 | DOI: 10.33393/dti.2025.3304 | Kaushik et al S. no. Sample ID Sample type Zn Staining AFB Culture GeneXpert CLAP-TB 1 NGS-07-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA NEGATIVE 2 NGS-02-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA NEGATIVE 3 NGS-28-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 4 NGS-29-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 5 NGS-41-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 6 NGS-18-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 7 NGS-34-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 8 NGS-45-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 9 MTB-138-SP Sputum NEGATIVE POSITIVE POSITIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 10 MTB-185-SP(MDR) Sputum NEGATIVE POSITIVE POSITIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 11 MTB-184-SP(MDR) Sputum NEGATIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 12 MTB-182-SP(MDR) Sputum NEGATIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 13 MTB-148-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 14 MTB-122-SP Sputum NEGATIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE Drug Target Insights 2025 | DOI: 10.33393/dti.2025.3304 | Kaushik et al 15 MTB-187-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA POSITIVE 16 MTB-116-SP Sputum NEGATIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 17 MTB-137-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA NEGATIVE 18 MTB-132-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA NEGATIVE 19 MTB-119- PUS Sputum NEGATIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 20 MTB-130-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 21 MTB-164-SP Sputum NEGATIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 22 MTB-143-SP(FLUID) Sputum Negative POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA POSITIVE 23 MTB-120-SP Sputum Negative POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 24 MTB-131-SP Sputum Negative Negative NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA NEGATIVE 25 MTB-123-SP Sputum Negative POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 26 MTB-133-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA NEGATIVE 27 MTB-188-SP Sputum NEGATIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 28 MTB-165-SP Sputum NEGATIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 29 MTB-141-SP Sputum NEGATIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Drug Target Insights 2025 | DOI: 10.33393/dti.2025.3304 | Kaushik et al Serum NA NA NA POSITIVE 30 MTB-135-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA NEGATIVE 31 MTB-134-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA NEGATIVE 32 MTB-125-SP Sputum NEGATIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 33 MTB-171-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 34 MTB-120-SP Sputum NEGATIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 35 MTB-113-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 36 MTB-129-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 37 MTB-121-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 38 MTB-186-SP(MDR) Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 39 MTB-124-SP Sputum NEGATIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 40 MTB-118-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 41 MTB-114-SP Sputum NEGATIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 42 MTB-117-SP Sputum NEGATIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 43 MTB-112-SP Sputum POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE Drug Target Insights 2025 | DOI: 10.33393/dti.2025.3304 | Kaushik et al 44 MTB-52-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 45 MTB-32-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine Sample not found Saple not Found Sample not found NA Serum NA NA NA POSITIVE 46 MTB-46-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 47 MTB-21-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 48 MTB-27-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 49 MTB-11-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 50 NGS-50-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 51 NGS-42-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 52 NGS-37-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 53 NGS-25-PUS Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 54 NGS24-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 55 NGS-30-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine Sample not found Sample not found Sample not found NA Serum NA NA NA POSITIVE 56 NGS-20-PUS Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 57 NGS-16-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE Drug Target Insights 2025 | DOI: 10.33393/dti.2025.3304 | Kaushik et al 58 NGS-22-GA Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 59 HEALTHY CONTROL- 1 Sputum NA NA NA NA Urine NEGATIVE NEGATIVE Not Done NEGATIVE Serum NA NA NA NEGATIVE 60 HEALTHY CONTROL- 2 Sputum NA NA NA NA Urine NEGATIVE NEGATIVE Not Done NEGATIVE Serum NA NA NA NEGATIVE 61 HEALTHY CONTROL- 3 Sputum NA NA NA NA Urine NEGATIVE NEGATIVE Not Done NEGATIVE Serum NA NA NA NEGATIVE 62 HEALTHY CONTROL- 4 Sputum NA NA NA NA Urine NEGATIVE NEGATIVE Not Done NEGATIVE Serum NA NA NA NEGATIVE 63 HEALTHY CONTROL- 5 Sputum NA NA NA NA Urine NEGATIVE NEGATIVE Not Done NEGATIVE Serum NEGATIVE NEGATIVE 64 HEALTHY CONTROL- 6 Sputum NA NA NA NA Urine NEGATIVE NEGATIVE Not Done NEGATIVE Serum NA NA NA NEGATIVE 65 HEALTHY CONTROL- 7 Sputum NA NA NA NA Urine NEGATIVE NEGATIVE Not Done NEGATIVE Serum NA NA NA NEGATIVE 66 HEALTHY CONTROL- 8 Sputum NA NA NA NA Urine NEGATIVE NEGATIVE Not Done NEGATIVE Serum NA NA NA NEGATIVE 67 HEALTHY CONTROL- 9 Sputum NA NA NA NA Urine NEGATIVE NEGATIVE Not Done NEGATIVE Serum NA NA NA NEGATIVE 68 HEALTHY CONTROL- 10 Sputum NA NA NA NA Urine NEGATIVE NEGATIVE Not Done NEGATIVE Serum NA NA NA NEGATIVE 69 NGS-03-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA NEGATIVE 70 NGS-04-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Drug Target Insights 2025 | DOI: 10.33393/dti.2025.3304 | Kaushik et al Serum NA NA NA NEGATIVE 71 NGS-02-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA NEGATIVE 72 NGS-06-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NEGATIVE NEGATIVE 73 NGS-09-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA NEGATIVE 74 MTB-128-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA NEGATIVE 75 MTB136-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA NEGATIVE 76 MTB-127-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA NEGATIVE 77 MTB-139-SP Sputum NEGATIVE NEGATIVE NEGATIVE NEGATIVE Urine NEGATIVE NEGATIVE NOT DONE NEGATIVE Serum NA NA NA NEGATIVE 78 NGS-23 Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 79 MTB-201 Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NA NA NA NA Serum NA NA NA NA 80 NGS-47-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 81 NGS-12-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE 82 NGS-38-SP Sputum POSITIVE POSITIVE POSITIVE POSITIVE Urine NEGATIVE NEGATIVE NOT DONE POSITIVE Serum NA NA NA POSITIVE