id	sid	tid	token	lemma	pos
elements-9604	1	1	a	a	DET
elements-9604	1	2	phage	phage	NOUN
elements-9604	1	3	display	display	NOUN
elements-9604	1	4	library	library	NOUN
elements-9604	1	5	was	be	AUX
elements-9604	1	6	previously	previously	ADV
elements-9604	1	7	constructed	construct	VERB
elements-9604	1	8	from	from	ADP
elements-9604	1	9	an	an	DET
elements-9604	1	10	siv	siv	NOUN
elements-9604	1	11	-	-	PUNCT
elements-9604	1	12	infected	infected	ADJ
elements-9604	1	13	rhesus	rhesus	NOUN
elements-9604	1	14	macaque	macaque	NOUN
elements-9604	1	15	.	.	PUNCT
elements-9604	2	1	several	several	ADJ
elements-9604	2	2	single	single	ADJ
elements-9604	2	3	chain	chain	NOUN
elements-9604	2	4	fv	fv	PROPN
elements-9604	2	5	(	(	PUNCT
elements-9604	2	6	scfv	scfv	PROPN
elements-9604	2	7	)	)	PUNCT
elements-9604	2	8	were	be	AUX
elements-9604	2	9	selected	select	VERB
elements-9604	2	10	using	use	VERB
elements-9604	2	11	phage	phage	NOUN
elements-9604	2	12	display	display	NOUN
elements-9604	2	13	technology	technology	NOUN
elements-9604	2	14	.	.	PUNCT
elements-9604	3	1	sequences	sequence	NOUN
elements-9604	3	2	corresponding	correspond	VERB
elements-9604	3	3	to	to	ADP
elements-9604	3	4	su24	su24	PROPN
elements-9604	3	5	,	,	PUNCT
elements-9604	3	6	su343	su343	PROPN
elements-9604	3	7	and	and	CCONJ
elements-9604	3	8	ll25x	ll25x	NOUN
elements-9604	3	9	were	be	AUX
elements-9604	3	10	optimized	optimize	VERB
elements-9604	3	11	for	for	ADP
elements-9604	3	12	expression	expression	NOUN
elements-9604	3	13	in	in	ADP
elements-9604	3	14	a	a	DET
elements-9604	3	15	mammalian	mammalian	ADJ
elements-9604	3	16	system	system	NOUN
elements-9604	3	17	and	and	CCONJ
elements-9604	3	18	commercially	commercially	ADV
elements-9604	3	19	synthesized	synthesize	VERB
elements-9604	3	20	.	.	PUNCT
elements-9604	4	1	in	in	ADP
elements-9604	4	2	this	this	DET
elements-9604	4	3	study	study	NOUN
elements-9604	4	4	,	,	PUNCT
elements-9604	4	5	we	we	PRON
elements-9604	4	6	aimed	aim	VERB
elements-9604	4	7	to	to	PART
elements-9604	4	8	characterize	characterize	VERB
elements-9604	4	9	the	the	DET
elements-9604	4	10	specificity	specificity	NOUN
elements-9604	4	11	of	of	ADP
elements-9604	4	12	su24	su24	PROPN
elements-9604	4	13	,	,	PUNCT
elements-9604	4	14	su343	su343	PROPN
elements-9604	4	15	,	,	PUNCT
elements-9604	4	16	and	and	CCONJ
elements-9604	4	17	ll25x	ll25x	NOUN
elements-9604	4	18	.	.	PUNCT
elements-9604	5	1	the	the	DET
elements-9604	5	2	codon	codon	NOUN
elements-9604	5	3	-	-	PUNCT
elements-9604	5	4	optimized	optimize	VERB
elements-9604	5	5	version	version	NOUN
elements-9604	5	6	of	of	ADP
elements-9604	5	7	the	the	DET
elements-9604	5	8	scfv	scfv	PROPN
elements-9604	5	9	ll25x	ll25x	PROPN
elements-9604	5	10	gene	gene	NOUN
elements-9604	5	11	sequence	sequence	NOUN
elements-9604	5	12	was	be	AUX
elements-9604	5	13	cloned	clone	VERB
elements-9604	5	14	into	into	ADP
elements-9604	5	15	a	a	DET
elements-9604	5	16	mammalian	mammalian	ADJ
elements-9604	5	17	expression	expression	NOUN
elements-9604	5	18	vector	vector	NOUN
elements-9604	5	19	(	(	PUNCT
elements-9604	5	20	pcep4	pcep4	NOUN
elements-9604	5	21	)	)	PUNCT
elements-9604	5	22	.	.	PUNCT
elements-9604	6	1	ll25x	ll25x	PROPN
elements-9604	6	2	dna	dna	PROPN
elements-9604	6	3	was	be	AUX
elements-9604	6	4	amplified	amplify	VERB
elements-9604	6	5	by	by	ADP
elements-9604	6	6	pcr	pcr	PROPN
elements-9604	6	7	,	,	PUNCT
elements-9604	6	8	and	and	CCONJ
elements-9604	6	9	the	the	DET
elements-9604	6	10	pcr	pcr	ADJ
elements-9604	6	11	product	product	NOUN
elements-9604	6	12	and	and	CCONJ
elements-9604	6	13	mammalian	mammalian	ADJ
elements-9604	6	14	expression	expression	NOUN
elements-9604	6	15	vector	vector	NOUN
elements-9604	6	16	were	be	AUX
elements-9604	6	17	both	both	PRON
elements-9604	6	18	digested	digest	VERB
elements-9604	6	19	with	with	ADP
elements-9604	6	20	kpni	kpni	NOUN
elements-9604	6	21	/	/	SYM
elements-9604	6	22	sapi	sapi	NOUN
elements-9604	6	23	restriction	restriction	NOUN
elements-9604	6	24	enzymes	enzyme	NOUN
elements-9604	6	25	.	.	PUNCT
elements-9604	7	1	the	the	DET
elements-9604	7	2	fragments	fragment	NOUN
elements-9604	7	3	were	be	AUX
elements-9604	7	4	ligated	ligate	VERB
elements-9604	7	5	using	use	VERB
elements-9604	7	6	t4dna	t4dna	PROPN
elements-9604	7	7	ligase	ligase	PROPN
elements-9604	7	8	.	.	PUNCT
elements-9604	8	1	e.	e.	PROPN
elements-9604	8	2	coli	coli	NOUN
elements-9604	8	3	cells	cell	NOUN
elements-9604	8	4	were	be	AUX
elements-9604	8	5	transformed	transform	VERB
elements-9604	8	6	with	with	ADP
elements-9604	8	7	the	the	DET
elements-9604	8	8	ligation	ligation	NOUN
elements-9604	8	9	reaction	reaction	NOUN
elements-9604	8	10	.	.	PUNCT
elements-9604	9	1	single	single	ADJ
elements-9604	9	2	colonies	colony	NOUN
elements-9604	9	3	were	be	AUX
elements-9604	9	4	selected	select	VERB
elements-9604	9	5	on	on	ADP
elements-9604	9	6	lb	lb	DET
elements-9604	9	7	agar	agar	NOUN
elements-9604	9	8	plates	plate	NOUN
elements-9604	9	9	containing	contain	VERB
elements-9604	9	10	the	the	DET
elements-9604	9	11	selective	selective	ADJ
elements-9604	9	12	antibiotic	antibiotic	ADJ
elements-9604	9	13	(	(	PUNCT
elements-9604	9	14	ampicillin	ampicillin	NOUN
elements-9604	9	15	)	)	PUNCT
elements-9604	9	16	.	.	PUNCT
elements-9604	10	1	positive	positive	ADJ
elements-9604	10	2	colonies	colony	NOUN
elements-9604	10	3	were	be	AUX
elements-9604	10	4	identified	identify	VERB
elements-9604	10	5	with	with	ADP
elements-9604	10	6	kpni	kpni	PROPN
elements-9604	10	7	and	and	CCONJ
elements-9604	10	8	sapi	sapi	NOUN
elements-9604	10	9	.	.	PUNCT
elements-9604	11	1	sanger	sanger	PROPN
elements-9604	11	2	sequencing	sequence	VERB
elements-9604	11	3	confirmed	confirm	VERB
elements-9604	11	4	cloning	cloning	NOUN
elements-9604	11	5	results	result	NOUN
elements-9604	11	6	and	and	CCONJ
elements-9604	11	7	dna	dna	NOUN
elements-9604	11	8	sequence	sequence	NOUN
elements-9604	11	9	accuracy	accuracy	NOUN
elements-9604	11	10	.	.	PUNCT
elements-9604	12	1	following	follow	VERB
elements-9604	12	2	transfection	transfection	NOUN
elements-9604	12	3	of	of	ADP
elements-9604	12	4	mammalian	mammalian	ADJ
elements-9604	12	5	cells	cell	NOUN
elements-9604	12	6	(	(	PUNCT
elements-9604	12	7	293	293	NUM
elements-9604	12	8	t	t	NOUN
elements-9604	12	9	)	)	PUNCT
elements-9604	12	10	,	,	PUNCT
elements-9604	12	11	ll25x	ll25x	NOUN
elements-9604	12	12	-	-	PUNCT
elements-9604	12	13	fc	fc	NOUN
elements-9604	12	14	cells	cell	NOUN
elements-9604	12	15	,	,	PUNCT
elements-9604	12	16	and	and	CCONJ
elements-9604	12	17	purifying	purify	VERB
elements-9604	12	18	our	our	PRON
elements-9604	12	19	protein	protein	NOUN
elements-9604	12	20	,	,	PUNCT
elements-9604	12	21	the	the	DET
elements-9604	12	22	binding	binding	NOUN
elements-9604	12	23	of	of	ADP
elements-9604	12	24	ll25x	ll25x	PROPN
elements-9604	12	25	-	-	PUNCT
elements-9604	12	26	fc	fc	PROPN
elements-9604	12	27	to	to	ADP
elements-9604	12	28	the	the	DET
elements-9604	12	29	siv	siv	PROPN
elements-9604	12	30	gp140	gp140	PROPN
elements-9604	12	31	envelope	envelope	NOUN
elements-9604	12	32	protein	protein	NOUN
elements-9604	12	33	was	be	AUX
elements-9604	12	34	confirmed	confirm	VERB
elements-9604	12	35	.	.	PUNCT
elements-9604	13	1	the	the	DET
elements-9604	13	2	road	road	NOUN
elements-9604	13	3	to	to	ADP
elements-9604	13	4	a	a	DET
elements-9604	13	5	cure	cure	NOUN
elements-9604	13	6	characterization	characterization	NOUN
elements-9604	13	7	of	of	ADP
elements-9604	13	8	a	a	DET
elements-9604	13	9	new	new	ADJ
elements-9604	13	10	hiv	hiv	NOUN
elements-9604	13	11	antibody	antibody	NOUN
elements-9604	13	12	zackary	zackary	NOUN
elements-9604	13	13	park	park	NOUN
elements-9604	13	14	,	,	PUNCT
elements-9604	13	15	seung	seung	PROPN
elements-9604	13	16	yub	yub	PROPN
elements-9604	13	17	han	han	PROPN
elements-9604	13	18	,	,	PUNCT
elements-9604	13	19	alexandrea	alexandrea	NOUN
elements-9604	13	20	ramnarine	ramnarine	NOUN
elements-9604	13	21	,	,	PUNCT
elements-9604	13	22	james	james	PROPN
elements-9604	13	23	kleinschmidt	kleinschmidt	PROPN
elements-9604	13	24	,	,	PUNCT
elements-9604	13	25	kimberlyn	kimberlyn	PROPN
elements-9604	13	26	austin	austin	PROPN
elements-9604	13	27	,	,	PUNCT
elements-9604	13	28	brendan	brendan	PROPN
elements-9604	13	29	hustead	hustead	PROPN
elements-9604	13	30	,	,	PUNCT
elements-9604	13	31	matthew	matthew	PROPN
elements-9604	13	32	slein	slein	PROPN
elements-9604	13	33	,	,	PUNCT
elements-9604	13	34	catherine	catherine	PROPN
elements-9604	13	35	phalen	phalen	PROPN
elements-9604	13	36	,	,	PUNCT
elements-9604	13	37	ariana	ariana	PROPN
elements-9604	13	38	gonzalez	gonzalez	PROPN
elements-9604	13	39	introduction	introduction	NOUN
elements-9604	13	40	since	since	SCONJ
elements-9604	13	41	the	the	DET
elements-9604	13	42	discovery	discovery	NOUN
elements-9604	13	43	of	of	ADP
elements-9604	13	44	hiv	hiv	PROPN
elements-9604	13	45	-	-	PUNCT
elements-9604	13	46	aids	aids	PROPN
elements-9604	13	47	in	in	ADP
elements-9604	13	48	1984	1984	NUM
elements-9604	13	49	,	,	PUNCT
elements-9604	13	50	an	an	DET
elements-9604	13	51	effective	effective	ADJ
elements-9604	13	52	vaccine	vaccine	NOUN
elements-9604	13	53	has	have	AUX
elements-9604	13	54	remained	remain	VERB
elements-9604	13	55	an	an	DET
elements-9604	13	56	elusive	elusive	ADJ
elements-9604	13	57	goal	goal	NOUN
elements-9604	13	58	,	,	PUNCT
elements-9604	13	59	despite	despite	SCONJ
elements-9604	13	60	the	the	DET
elements-9604	13	61	conjugation	conjugation	NOUN
elements-9604	13	62	of	of	ADP
elements-9604	13	63	numerous	numerous	ADJ
elements-9604	13	64	efforts	effort	NOUN
elements-9604	13	65	.	.	PUNCT
elements-9604	14	1	progress	progress	NOUN
elements-9604	14	2	made	make	VERB
elements-9604	14	3	in	in	ADP
elements-9604	14	4	the	the	DET
elements-9604	14	5	rv144	rv144	PROPN
elements-9604	14	6	thai	thai	ADJ
elements-9604	14	7	vaccine	vaccine	NOUN
elements-9604	14	8	trial	trial	NOUN
elements-9604	14	9	has	have	AUX
elements-9604	14	10	hinted	hint	VERB
elements-9604	14	11	that	that	SCONJ
elements-9604	14	12	hiv	hiv	PROPN
elements-9604	14	13	envelope	envelope	NOUN
elements-9604	14	14	glycoprotein	glycoprotein	NOUN
elements-9604	14	15	variable	variable	PROPN
elements-9604	14	16	loop	loop	NOUN
elements-9604	14	17	v1	v1	PROPN
elements-9604	14	18	-	-	PUNCT
elements-9604	14	19	v2	v2	NOUN
elements-9604	14	20	has	have	VERB
elements-9604	14	21	a	a	DET
elements-9604	14	22	role	role	NOUN
elements-9604	14	23	in	in	ADP
elements-9604	14	24	the	the	DET
elements-9604	14	25	prevention	prevention	NOUN
elements-9604	14	26	of	of	ADP
elements-9604	14	27	infection.1	infection.1	NOUN
elements-9604	14	28	the	the	DET
elements-9604	14	29	isolation	isolation	NOUN
elements-9604	14	30	of	of	ADP
elements-9604	14	31	anti	anti	ADJ
elements-9604	14	32	-	-	ADJ
elements-9604	14	33	siv	siv	ADJ
elements-9604	14	34	monoclonal	monoclonal	NOUN
elements-9604	14	35	antibodies	antibody	NOUN
elements-9604	14	36	(	(	PUNCT
elements-9604	14	37	mab	mab	NOUN
elements-9604	14	38	)	)	PUNCT
elements-9604	14	39	will	will	AUX
elements-9604	14	40	allow	allow	VERB
elements-9604	14	41	a	a	DET
elements-9604	14	42	pre	pre	ADJ
elements-9604	14	43	-	-	ADJ
elements-9604	14	44	clinical	clinical	ADJ
elements-9604	14	45	evaluation	evaluation	NOUN
elements-9604	14	46	of	of	ADP
elements-9604	14	47	such	such	ADJ
elements-9604	14	48	antibodies	antibody	NOUN
elements-9604	14	49	in	in	ADP
elements-9604	14	50	the	the	DET
elements-9604	14	51	siv	siv	NOUN
elements-9604	14	52	-	-	PUNCT
elements-9604	14	53	rhesus	rhesus	NOUN
elements-9604	14	54	macaque	macaque	ADJ
elements-9604	14	55	model	model	NOUN
elements-9604	14	56	of	of	ADP
elements-9604	14	57	hiv	hiv	PROPN
elements-9604	14	58	/	/	SYM
elements-9604	14	59	aids	aids	PROPN
elements-9604	14	60	pathogenesis	pathogenesis	NOUN
elements-9604	14	61	and	and	CCONJ
elements-9604	14	62	vaccine	vaccine	NOUN
elements-9604	14	63	.	.	PUNCT
elements-9604	15	1	a	a	DET
elements-9604	15	2	phage	phage	NOUN
elements-9604	15	3	display	display	NOUN
elements-9604	15	4	library	library	NOUN
elements-9604	15	5	was	be	AUX
elements-9604	15	6	previously	previously	ADV
elements-9604	15	7	constructed	construct	VERB
elements-9604	15	8	from	from	ADP
elements-9604	15	9	a	a	DET
elements-9604	15	10	siv	siv	NOUN
elements-9604	15	11	-	-	PUNCT
elements-9604	15	12	infected	infected	ADJ
elements-9604	15	13	rhesus	rhesus	NOUN
elements-9604	15	14	macaque	macaque	NOUN
elements-9604	15	15	,	,	PUNCT
elements-9604	15	16	and	and	CCONJ
elements-9604	15	17	several	several	ADJ
elements-9604	15	18	single	single	ADJ
elements-9604	15	19	chain	chain	NOUN
elements-9604	15	20	variable	variable	ADJ
elements-9604	15	21	fragments	fragment	NOUN
elements-9604	15	22	(	(	PUNCT
elements-9604	15	23	scfv	scfv	PROPN
elements-9604	15	24	)	)	PUNCT
elements-9604	15	25	such	such	ADJ
elements-9604	15	26	as	as	ADP
elements-9604	15	27	su24	su24	PROPN
elements-9604	15	28	,	,	PUNCT
elements-9604	15	29	su343	su343	PROPN
elements-9604	15	30	,	,	PUNCT
elements-9604	15	31	and	and	CCONJ
elements-9604	15	32	ll25x	ll25x	NOUN
elements-9604	15	33	were	be	AUX
elements-9604	15	34	selected	select	VERB
elements-9604	15	35	using	use	VERB
elements-9604	15	36	phage	phage	NOUN
elements-9604	15	37	display	display	NOUN
elements-9604	15	38	technology.2	technology.2	PROPN
elements-9604	15	39	sequences	sequence	NOUN
elements-9604	15	40	corresponding	correspond	VERB
elements-9604	15	41	to	to	ADP
elements-9604	15	42	su24	su24	PROPN
elements-9604	15	43	,	,	PUNCT
elements-9604	15	44	su343	su343	PROPN
elements-9604	15	45	,	,	PUNCT
elements-9604	15	46	and	and	CCONJ
elements-9604	15	47	ll25x	ll25x	NOUN
elements-9604	15	48	were	be	AUX
elements-9604	15	49	optimized	optimize	VERB
elements-9604	15	50	for	for	ADP
elements-9604	15	51	expression	expression	NOUN
elements-9604	15	52	in	in	ADP
elements-9604	15	53	a	a	DET
elements-9604	15	54	mammalian	mammalian	ADJ
elements-9604	15	55	system	system	NOUN
elements-9604	15	56	and	and	CCONJ
elements-9604	15	57	commercially	commercially	ADV
elements-9604	15	58	synthesized	synthesize	VERB
elements-9604	15	59	(	(	PUNCT
elements-9604	15	60	idt	idt	PROPN
elements-9604	15	61	dna	dna	PROPN
elements-9604	15	62	)	)	PUNCT
elements-9604	15	63	.	.	PUNCT
elements-9604	16	1	su24	su24	PROPN
elements-9604	16	2	and	and	CCONJ
elements-9604	16	3	su343	su343	PROPN
elements-9604	16	4	had	have	AUX
elements-9604	16	5	previously	previously	ADV
elements-9604	16	6	been	be	AUX
elements-9604	16	7	cloned	clone	VERB
elements-9604	16	8	in	in	ADP
elements-9604	16	9	a	a	DET
elements-9604	16	10	mammalian	mammalian	ADJ
elements-9604	16	11	expression	expression	NOUN
elements-9604	16	12	vector	vector	NOUN
elements-9604	16	13	.	.	PUNCT
elements-9604	17	1	in	in	ADP
elements-9604	17	2	this	this	DET
elements-9604	17	3	study	study	NOUN
elements-9604	17	4	,	,	PUNCT
elements-9604	17	5	we	we	PRON
elements-9604	17	6	aimed	aim	VERB
elements-9604	17	7	to	to	PART
elements-9604	17	8	characterize	characterize	VERB
elements-9604	17	9	the	the	DET
elements-9604	17	10	specificity	specificity	NOUN
elements-9604	17	11	of	of	ADP
elements-9604	17	12	su24	su24	PROPN
elements-9604	17	13	,	,	PUNCT
elements-9604	17	14	su343	su343	PROPN
elements-9604	17	15	,	,	PUNCT
elements-9604	17	16	and	and	CCONJ
elements-9604	17	17	ll25x	ll25x	NOUN
elements-9604	17	18	.	.	PUNCT
elements-9604	18	1	materials	material	NOUN
elements-9604	18	2	and	and	CCONJ
elements-9604	18	3	methods	method	NOUN
elements-9604	18	4	pcr	pcr	PROPN
elements-9604	18	5	(	(	PUNCT
elements-9604	18	6	polymerase	polymerase	NOUN
elements-9604	18	7	chain	chain	NOUN
elements-9604	18	8	reaction	reaction	NOUN
elements-9604	18	9	)	)	PUNCT
elements-9604	18	10	amplification	amplification	NOUN
elements-9604	18	11	the	the	DET
elements-9604	18	12	codon	codon	NOUN
elements-9604	18	13	-	-	PUNCT
elements-9604	18	14	optimized	optimize	VERB
elements-9604	18	15	version	version	NOUN
elements-9604	18	16	of	of	ADP
elements-9604	18	17	the	the	DET
elements-9604	18	18	ll25x	ll25x	PROPN
elements-9604	18	19	gene	gene	NOUN
elements-9604	18	20	was	be	AUX
elements-9604	18	21	to	to	PART
elements-9604	18	22	be	be	AUX
elements-9604	18	23	amplified	amplify	VERB
elements-9604	18	24	by	by	ADP
elements-9604	18	25	pcr	pcr	PROPN
elements-9604	18	26	.	.	PUNCT
elements-9604	19	1	a	a	DET
elements-9604	19	2	pcr	pcr	ADJ
elements-9604	19	3	mixture	mixture	NOUN
elements-9604	19	4	was	be	AUX
elements-9604	19	5	prepared	prepare	VERB
elements-9604	19	6	in	in	ADP
elements-9604	19	7	a	a	DET
elements-9604	19	8	pcr	pcr	ADJ
elements-9604	19	9	tube	tube	NOUN
elements-9604	19	10	.	.	PUNCT
elements-9604	20	1	the	the	DET
elements-9604	20	2	pcr	pcr	PROPN
elements-9604	20	3	mixture	mixture	NOUN
elements-9604	20	4	consisted	consist	VERB
elements-9604	20	5	of	of	ADP
elements-9604	20	6	1	1	NUM
elements-9604	20	7	µl	µl	NOUN
elements-9604	20	8	of	of	ADP
elements-9604	20	9	template	template	NOUN
elements-9604	20	10	dna	dna	NOUN
elements-9604	20	11	(	(	PUNCT
elements-9604	20	12	100	100	NUM
elements-9604	20	13	ng	ng	PROPN
elements-9604	20	14	1	1	NUM
elements-9604	20	15	µg	µg	NOUN
elements-9604	20	16	)	)	PUNCT
elements-9604	20	17	,	,	PUNCT
elements-9604	20	18	2	2	NUM
elements-9604	20	19	µl	µl	NOUN
elements-9604	20	20	of	of	ADP
elements-9604	20	21	forward	forward	ADJ
elements-9604	20	22	primer	primer	NOUN
elements-9604	20	23	(	(	PUNCT
elements-9604	20	24	fp	fp	NOUN
elements-9604	20	25	)	)	PUNCT
elements-9604	20	26	,	,	PUNCT
elements-9604	20	27	2	2	NUM
elements-9604	20	28	µl	µl	NOUN
elements-9604	20	29	of	of	ADP
elements-9604	20	30	reverse	reverse	ADJ
elements-9604	20	31	primer	primer	NOUN
elements-9604	20	32	(	(	PUNCT
elements-9604	20	33	rp	rp	NOUN
elements-9604	20	34	)	)	PUNCT
elements-9604	20	35	,	,	PUNCT
elements-9604	20	36	and	and	CCONJ
elements-9604	20	37	45	45	NUM
elements-9604	20	38	µl	µl	NOUN
elements-9604	20	39	of	of	ADP
elements-9604	20	40	platinum	platinum	NOUN
elements-9604	20	41	pcr	pcr	ADJ
elements-9604	20	42	supermix	supermix	NOUN
elements-9604	20	43	high	high	ADJ
elements-9604	20	44	fidelity	fidelity	NOUN
elements-9604	20	45	from	from	ADP
elements-9604	20	46	thermo	thermo	PROPN
elements-9604	20	47	fisher	fisher	PROPN
elements-9604	20	48	scientific	scientific	PROPN
elements-9604	20	49	.	.	PUNCT
elements-9604	21	1	the	the	DET
elements-9604	21	2	forward	forward	ADJ
elements-9604	21	3	primer	primer	NOUN
elements-9604	21	4	was	be	AUX
elements-9604	21	5	ll25	ll25	PROPN
elements-9604	21	6	-	-	PUNCT
elements-9604	21	7	co	co	NOUN
elements-9604	21	8	-	-	NOUN
elements-9604	21	9	f	f	X
elements-9604	21	10	(	(	PUNCT
elements-9604	21	11	ggccggtaccggcgcgccaccatgg	ggccggtaccggcgcgccaccatgg	PROPN
elements-9604	21	12	)	)	PUNCT
elements-9604	21	13	and	and	CCONJ
elements-9604	21	14	included	include	VERB
elements-9604	21	15	the	the	DET
elements-9604	21	16	restriction	restriction	NOUN
elements-9604	21	17	site	site	NOUN
elements-9604	21	18	for	for	ADP
elements-9604	21	19	kpni	kpni	PROPN
elements-9604	21	20	.	.	PUNCT
elements-9604	22	1	the	the	DET
elements-9604	22	2	reverse	reverse	ADJ
elements-9604	22	3	primer	primer	NOUN
elements-9604	22	4	was	be	AUX
elements-9604	22	5	ll25	ll25	PROPN
elements-9604	22	6	-	-	PUNCT
elements-9604	22	7	co	co	NOUN
elements-9604	22	8	-	-	NOUN
elements-9604	22	9	r	r	NOUN
elements-9604	22	10	(	(	PUNCT
elements-9604	22	11	attgtactgctcttcccgtggcttggttttcgc	attgtactgctcttcccgtggcttggttttcgc	NOUN
elements-9604	22	12	)	)	PUNCT
elements-9604	22	13	and	and	CCONJ
elements-9604	22	14	included	include	VERB
elements-9604	22	15	the	the	DET
elements-9604	22	16	restriction	restriction	NOUN
elements-9604	22	17	site	site	NOUN
elements-9604	22	18	for	for	ADP
elements-9604	22	19	sapi	sapi	NOUN
elements-9604	22	20	.	.	PUNCT
elements-9604	23	1	the	the	DET
elements-9604	23	2	pcr	pcr	ADJ
elements-9604	23	3	tube	tube	NOUN
elements-9604	23	4	was	be	AUX
elements-9604	23	5	closed	close	VERB
elements-9604	23	6	tightly	tightly	ADV
elements-9604	23	7	,	,	PUNCT
elements-9604	23	8	mixed	mix	VERB
elements-9604	23	9	via	via	ADP
elements-9604	23	10	vortex	vortex	NOUN
elements-9604	23	11	,	,	PUNCT
elements-9604	23	12	and	and	CCONJ
elements-9604	23	13	spun	spin	VERB
elements-9604	23	14	down	down	ADP
elements-9604	23	15	using	use	VERB
elements-9604	23	16	a	a	DET
elements-9604	23	17	small	small	ADJ
elements-9604	23	18	bench	bench	NOUN
elements-9604	23	19	-	-	PUNCT
elements-9604	23	20	top	top	NOUN
elements-9604	23	21	rotor	rotor	NOUN
elements-9604	23	22	.	.	PUNCT
elements-9604	24	1	the	the	DET
elements-9604	24	2	pcr	pcr	ADJ
elements-9604	24	3	tube	tube	NOUN
elements-9604	24	4	was	be	AUX
elements-9604	24	5	placed	place	VERB
elements-9604	24	6	in	in	ADP
elements-9604	24	7	a	a	DET
elements-9604	24	8	thermocycler	thermocycler	NOUN
elements-9604	24	9	for	for	ADP
elements-9604	24	10	25	25	NUM
elements-9604	24	11	cycles	cycle	NOUN
elements-9604	24	12	.	.	PUNCT
elements-9604	25	1	the	the	DET
elements-9604	25	2	initial	initial	ADJ
elements-9604	25	3	denaturation	denaturation	NOUN
elements-9604	25	4	step	step	NOUN
elements-9604	25	5	was	be	AUX
elements-9604	25	6	performed	perform	VERB
elements-9604	25	7	at	at	ADP
elements-9604	25	8	94	94	NUM
elements-9604	25	9	°	°	ADP
elements-9604	25	10	c	c	NOUN
elements-9604	25	11	for	for	ADP
elements-9604	25	12	5	5	NUM
elements-9604	25	13	minutes	minute	NOUN
elements-9604	25	14	and	and	CCONJ
elements-9604	25	15	was	be	AUX
elements-9604	25	16	followed	follow	VERB
elements-9604	25	17	by	by	ADP
elements-9604	25	18	25	25	NUM
elements-9604	25	19	cycles	cycle	NOUN
elements-9604	25	20	of	of	ADP
elements-9604	25	21	denaturation	denaturation	NOUN
elements-9604	25	22	(	(	PUNCT
elements-9604	25	23	94	94	NUM
elements-9604	25	24	°	°	ADP
elements-9604	25	25	c	c	NOUN
elements-9604	25	26	for	for	ADP
elements-9604	25	27	30	30	NUM
elements-9604	25	28	seconds	second	NOUN
elements-9604	25	29	)	)	PUNCT
elements-9604	25	30	,	,	PUNCT
elements-9604	25	31	annealing	anneal	VERB
elements-9604	25	32	(	(	PUNCT
elements-9604	25	33	50	50	NUM
elements-9604	25	34	°	°	NOUN
elements-9604	25	35	c	c	NOUN
elements-9604	25	36	for	for	ADP
elements-9604	25	37	30	30	NUM
elements-9604	25	38	seconds	second	NOUN
elements-9604	25	39	)	)	PUNCT
elements-9604	25	40	,	,	PUNCT
elements-9604	25	41	and	and	CCONJ
elements-9604	25	42	extension	extension	NOUN
elements-9604	25	43	(	(	PUNCT
elements-9604	25	44	68	68	NUM
elements-9604	25	45	°	°	NOUN
elements-9604	25	46	c	c	NOUN
elements-9604	25	47	for	for	ADP
elements-9604	25	48	1	1	NUM
elements-9604	25	49	minute	minute	NOUN
elements-9604	25	50	)	)	PUNCT
elements-9604	25	51	.	.	PUNCT
elements-9604	26	1	a	a	DET
elements-9604	26	2	final	final	ADJ
elements-9604	26	3	extension	extension	NOUN
elements-9604	26	4	step	step	NOUN
elements-9604	26	5	was	be	AUX
elements-9604	26	6	performed	perform	VERB
elements-9604	26	7	at	at	ADP
elements-9604	26	8	62	62	NUM
elements-9604	26	9	°	°	ADP
elements-9604	26	10	c	c	NOUN
elements-9604	26	11	for	for	ADP
elements-9604	26	12	10	10	NUM
elements-9604	26	13	minutes	minute	NOUN
elements-9604	26	14	.	.	PUNCT
elements-9604	27	1	dna	dna	PROPN
elements-9604	27	2	gel	gel	NOUN
elements-9604	27	3	electrophoresis	electrophoresis	NOUN
elements-9604	27	4	of	of	ADP
elements-9604	27	5	the	the	DET
elements-9604	27	6	pcr	pcr	ADJ
elements-9604	27	7	products	product	NOUN
elements-9604	27	8	was	be	AUX
elements-9604	27	9	performed	perform	VERB
elements-9604	27	10	on	on	ADP
elements-9604	27	11	1	1	NUM
elements-9604	27	12	%	%	NOUN
elements-9604	27	13	agarose	agarose	X
elements-9604	27	14	gel	gel	NOUN
elements-9604	27	15	.	.	PUNCT
elements-9604	28	1	the	the	DET
elements-9604	28	2	dna	dna	PROPN
elements-9604	28	3	vector	vector	NOUN
elements-9604	28	4	was	be	AUX
elements-9604	28	5	observed	observe	VERB
elements-9604	28	6	around	around	ADP
elements-9604	28	7	1092	1092	NUM
elements-9604	28	8	bp	bp	PROPN
elements-9604	28	9	under	under	ADP
elements-9604	28	10	a	a	DET
elements-9604	28	11	uv	uv	NOUN
elements-9604	28	12	transilluminator	transilluminator	NOUN
elements-9604	28	13	and	and	CCONJ
elements-9604	28	14	was	be	AUX
elements-9604	28	15	extracted	extract	VERB
elements-9604	28	16	using	use	VERB
elements-9604	28	17	a	a	DET
elements-9604	28	18	gel	gel	NOUN
elements-9604	28	19	extraction	extraction	NOUN
elements-9604	28	20	kit	kit	NOUN
elements-9604	28	21	(	(	PUNCT
elements-9604	28	22	qiagen	qiagen	PROPN
elements-9604	28	23	)	)	PUNCT
elements-9604	28	24	.	.	PUNCT
elements-9604	29	1	extraction	extraction	NOUN
elements-9604	29	2	of	of	ADP
elements-9604	29	3	pcr	pcr	ADJ
elements-9604	29	4	products	product	NOUN
elements-9604	29	5	the	the	DET
elements-9604	29	6	pcr	pcr	ADJ
elements-9604	29	7	products	product	NOUN
elements-9604	29	8	were	be	AUX
elements-9604	29	9	extracted	extract	VERB
elements-9604	29	10	from	from	ADP
elements-9604	29	11	the	the	DET
elements-9604	29	12	1	1	NUM
elements-9604	29	13	%	%	NOUN
elements-9604	29	14	agarose	agarose	NOUN
elements-9604	29	15	gel	gel	NOUN
elements-9604	29	16	following	follow	VERB
elements-9604	29	17	the	the	DET
elements-9604	29	18	qiaquick	qiaquick	NOUN
elements-9604	29	19	gel	gel	NOUN
elements-9604	29	20	extraction	extraction	NOUN
elements-9604	29	21	kit	kit	NOUN
elements-9604	29	22	by	by	ADP
elements-9604	29	23	qiagen	qiagen	PROPN
elements-9604	29	24	.	.	PUNCT
elements-9604	30	1	instead	instead	ADV
elements-9604	30	2	of	of	ADP
elements-9604	30	3	the	the	DET
elements-9604	30	4	recommended	recommend	VERB
elements-9604	30	5	50	50	NUM
elements-9604	30	6	µl	µl	NOUN
elements-9604	30	7	of	of	ADP
elements-9604	30	8	buffer	buffer	NOUN
elements-9604	30	9	eb	eb	PROPN
elements-9604	30	10	,	,	PUNCT
elements-9604	30	11	30	30	NUM
elements-9604	30	12	µl	µl	NOUN
elements-9604	30	13	of	of	ADP
elements-9604	30	14	double	double	ADJ
elements-9604	30	15	distilled	distil	VERB
elements-9604	30	16	water	water	NOUN
elements-9604	30	17	was	be	AUX
elements-9604	30	18	added	add	VERB
elements-9604	30	19	to	to	ADP
elements-9604	30	20	the	the	DET
elements-9604	30	21	qiaquick	qiaquick	NOUN
elements-9604	30	22	membrane	membrane	NOUN
elements-9604	30	23	column	column	NOUN
elements-9604	30	24	to	to	PART
elements-9604	30	25	elute	elute	VERB
elements-9604	30	26	dna	dna	NOUN
elements-9604	30	27	and	and	CCONJ
elements-9604	30	28	centrifuged	centrifuge	VERB
elements-9604	30	29	immediately	immediately	ADV
elements-9604	30	30	.	.	PUNCT
elements-9604	31	1	loading	load	VERB
elements-9604	31	2	dye	dye	NOUN
elements-9604	31	3	was	be	AUX
elements-9604	31	4	not	not	PART
elements-9604	31	5	added	add	VERB
elements-9604	31	6	to	to	ADP
elements-9604	31	7	purified	purified	ADJ
elements-9604	31	8	dna	dna	PROPN
elements-9604	31	9	.	.	PUNCT
elements-9604	32	1	double	double	ADJ
elements-9604	32	2	digestion	digestion	NOUN
elements-9604	32	3	of	of	ADP
elements-9604	32	4	pcr	pcr	ADJ
elements-9604	32	5	products	product	NOUN
elements-9604	32	6	with	with	ADP
elements-9604	32	7	kpni	kpni	NOUN
elements-9604	32	8	and	and	CCONJ
elements-9604	32	9	sapi	sapi	VERB
elements-9604	32	10	after	after	ADP
elements-9604	32	11	gel	gel	NOUN
elements-9604	32	12	extraction	extraction	NOUN
elements-9604	32	13	,	,	PUNCT
elements-9604	32	14	the	the	DET
elements-9604	32	15	dna	dna	PROPN
elements-9604	32	16	vector	vector	NOUN
elements-9604	32	17	from	from	ADP
elements-9604	32	18	pcr	pcr	NOUN
elements-9604	32	19	was	be	AUX
elements-9604	32	20	double	double	ADV
elements-9604	32	21	digested	digest	VERB
elements-9604	32	22	with	with	ADP
elements-9604	32	23	kpni	kpni	NOUN
elements-9604	32	24	and	and	CCONJ
elements-9604	32	25	sapi	sapi	NOUN
elements-9604	32	26	.	.	PUNCT
elements-9604	33	1	the	the	DET
elements-9604	33	2	double	double	ADJ
elements-9604	33	3	digest	digest	NOUN
elements-9604	33	4	reaction	reaction	NOUN
elements-9604	33	5	mixture	mixture	NOUN
elements-9604	33	6	consisted	consist	VERB
elements-9604	33	7	of	of	ADP
elements-9604	33	8	30	30	NUM
elements-9604	33	9	µl	µl	NOUN
elements-9604	33	10	of	of	ADP
elements-9604	33	11	the	the	DET
elements-9604	33	12	dna	dna	PROPN
elements-9604	33	13	vector	vector	NOUN
elements-9604	33	14	,	,	PUNCT
elements-9604	33	15	4	4	NUM
elements-9604	33	16	µl	µl	NOUN
elements-9604	33	17	of	of	ADP
elements-9604	33	18	cutsmart	cutsmart	ADJ
elements-9604	33	19	buffer	buffer	NOUN
elements-9604	33	20	from	from	ADP
elements-9604	33	21	new	new	PROPN
elements-9604	33	22	england	england	PROPN
elements-9604	33	23	biolabs	biolab	NOUN
elements-9604	33	24	,	,	PUNCT
elements-9604	33	25	1	1	NUM
elements-9604	33	26	µl	µl	ADP
elements-9604	33	27	of	of	ADP
elements-9604	33	28	kpni	kpni	NOUN
elements-9604	33	29	,	,	PUNCT
elements-9604	33	30	1	1	NUM
elements-9604	33	31	µl	µl	ADP
elements-9604	33	32	of	of	ADP
elements-9604	33	33	sapi	sapi	NOUN
elements-9604	33	34	,	,	PUNCT
elements-9604	33	35	and	and	CCONJ
elements-9604	33	36	4	4	NUM
elements-9604	33	37	µl	µl	NOUN
elements-9604	33	38	of	of	ADP
elements-9604	33	39	double	double	ADJ
elements-9604	33	40	distilled	distil	VERB
elements-9604	33	41	water	water	NOUN
elements-9604	33	42	for	for	ADP
elements-9604	33	43	a	a	DET
elements-9604	33	44	total	total	ADJ
elements-9604	33	45	concentration	concentration	NOUN
elements-9604	33	46	of	of	ADP
elements-9604	33	47	40	40	NUM
elements-9604	33	48	µl	µl	NOUN
elements-9604	33	49	.	.	PUNCT
elements-9604	34	1	dna	dna	PROPN
elements-9604	34	2	gel	gel	NOUN
elements-9604	34	3	electrophoresis	electrophoresis	NOUN
elements-9604	34	4	of	of	ADP
elements-9604	34	5	the	the	DET
elements-9604	34	6	double	double	ADJ
elements-9604	34	7	digested	digest	VERB
elements-9604	34	8	ll25x	ll25x	NOUN
elements-9604	34	9	pcr	pcr	PROPN
elements-9604	34	10	product	product	NOUN
elements-9604	34	11	was	be	AUX
elements-9604	34	12	performed	perform	VERB
elements-9604	34	13	on	on	ADP
elements-9604	34	14	1	1	NUM
elements-9604	34	15	%	%	NOUN
elements-9604	34	16	agarose	agarose	NOUN
elements-9604	34	17	gel	gel	NOUN
elements-9604	34	18	for	for	ADP
elements-9604	34	19	1	1	NUM
elements-9604	34	20	hour	hour	NOUN
elements-9604	34	21	.	.	PUNCT
elements-9604	35	1	extraction	extraction	NOUN
elements-9604	35	2	of	of	ADP
elements-9604	35	3	pcr	pcr	ADJ
elements-9604	35	4	fragment	fragment	NOUN
elements-9604	35	5	double	double	ADV
elements-9604	35	6	digested	digest	VERB
elements-9604	35	7	with	with	ADP
elements-9604	35	8	kpni	kpni	NOUN
elements-9604	35	9	and	and	CCONJ
elements-9604	35	10	sapi	sapi	VERB
elements-9604	35	11	the	the	DET
elements-9604	35	12	dna	dna	NOUN
elements-9604	35	13	of	of	ADP
elements-9604	35	14	the	the	DET
elements-9604	35	15	insert	insert	ADJ
elements-9604	35	16	vector	vector	NOUN
elements-9604	35	17	resulting	result	VERB
elements-9604	35	18	from	from	ADP
elements-9604	35	19	the	the	DET
elements-9604	35	20	pcr	pcr	ADJ
elements-9604	35	21	fragment	fragment	NOUN
elements-9604	35	22	double	double	ADV
elements-9604	35	23	digested	digest	VERB
elements-9604	35	24	with	with	ADP
elements-9604	35	25	kpni	kpni	PROPN
elements-9604	35	26	and	and	CCONJ
elements-9604	35	27	sapi	sapi	NOUN
elements-9604	35	28	was	be	AUX
elements-9604	35	29	extracted	extract	VERB
elements-9604	35	30	from	from	ADP
elements-9604	35	31	the	the	DET
elements-9604	35	32	1	1	NUM
elements-9604	35	33	%	%	NOUN
elements-9604	35	34	agarose	agarose	NOUN
elements-9604	35	35	gel	gel	NOUN
elements-9604	35	36	using	use	VERB
elements-9604	35	37	the	the	DET
elements-9604	35	38	qiaquick	qiaquick	NOUN
elements-9604	35	39	gel	gel	NOUN
elements-9604	35	40	extraction	extraction	NOUN
elements-9604	35	41	kit	kit	NOUN
elements-9604	35	42	protocol	protocol	NOUN
elements-9604	35	43	by	by	ADP
elements-9604	35	44	qiagen	qiagen	PROPN
elements-9604	35	45	.	.	PUNCT
elements-9604	36	1	instead	instead	ADV
elements-9604	36	2	of	of	ADP
elements-9604	36	3	the	the	DET
elements-9604	36	4	recommended	recommend	VERB
elements-9604	36	5	50	50	NUM
elements-9604	36	6	µl	µl	NOUN
elements-9604	36	7	of	of	ADP
elements-9604	36	8	buffer	buffer	NOUN
elements-9604	36	9	eb	eb	PROPN
elements-9604	36	10	,	,	PUNCT
elements-9604	36	11	30	30	NUM
elements-9604	36	12	µl	µl	NOUN
elements-9604	36	13	of	of	ADP
elements-9604	36	14	double	double	ADJ
elements-9604	36	15	distilled	distil	VERB
elements-9604	36	16	water	water	NOUN
elements-9604	36	17	was	be	AUX
elements-9604	36	18	added	add	VERB
elements-9604	36	19	to	to	ADP
elements-9604	36	20	the	the	DET
elements-9604	36	21	qiaquick	qiaquick	NOUN
elements-9604	36	22	membrane	membrane	NOUN
elements-9604	36	23	column	column	NOUN
elements-9604	36	24	to	to	PART
elements-9604	36	25	elute	elute	VERB
elements-9604	36	26	dna	dna	NOUN
elements-9604	36	27	and	and	CCONJ
elements-9604	36	28	centrifuged	centrifuge	VERB
elements-9604	36	29	immediately	immediately	ADV
elements-9604	36	30	.	.	PUNCT
elements-9604	37	1	loading	load	VERB
elements-9604	37	2	dye	dye	NOUN
elements-9604	37	3	was	be	AUX
elements-9604	37	4	not	not	PART
elements-9604	37	5	added	add	VERB
elements-9604	37	6	to	to	ADP
elements-9604	37	7	purified	purified	ADJ
elements-9604	37	8	dna	dna	PROPN
elements-9604	37	9	.	.	PUNCT
elements-9604	38	1	double	double	ADJ
elements-9604	38	2	digestion	digestion	NOUN
elements-9604	38	3	of	of	ADP
elements-9604	38	4	mammalian	mammalian	ADJ
elements-9604	38	5	expression	expression	NOUN
elements-9604	38	6	vector	vector	NOUN
elements-9604	38	7	pcep4	pcep4	NOUN
elements-9604	38	8	with	with	ADP
elements-9604	38	9	kpni	kpni	NOUN
elements-9604	38	10	and	and	CCONJ
elements-9604	38	11	sapi	sapi	VERB
elements-9604	38	12	three	three	NUM
elements-9604	38	13	samples	sample	NOUN
elements-9604	38	14	were	be	AUX
elements-9604	38	15	prepared	prepare	VERB
elements-9604	38	16	for	for	ADP
elements-9604	38	17	the	the	DET
elements-9604	38	18	double	double	ADJ
elements-9604	38	19	digest	digest	NOUN
elements-9604	38	20	with	with	ADP
elements-9604	38	21	kpni	kpni	NOUN
elements-9604	38	22	and	and	CCONJ
elements-9604	38	23	sapi	sapi	NOUN
elements-9604	38	24	was	be	AUX
elements-9604	38	25	performed	perform	VERB
elements-9604	38	26	on	on	ADP
elements-9604	38	27	the	the	DET
elements-9604	38	28	mammalian	mammalian	ADJ
elements-9604	38	29	expression	expression	NOUN
elements-9604	38	30	vector	vector	NOUN
elements-9604	38	31	pcep4	pcep4	NOUN
elements-9604	38	32	.	.	PUNCT
elements-9604	39	1	one	one	NUM
elements-9604	39	2	sample	sample	NOUN
elements-9604	39	3	was	be	AUX
elements-9604	39	4	the	the	DET
elements-9604	39	5	reaction	reaction	NOUN
elements-9604	39	6	mix	mix	NOUN
elements-9604	39	7	for	for	ADP
elements-9604	39	8	the	the	DET
elements-9604	39	9	double	double	ADJ
elements-9604	39	10	digest	digest	NOUN
elements-9604	39	11	consisted	consist	VERB
elements-9604	39	12	of	of	ADP
elements-9604	39	13	1	1	NUM
elements-9604	39	14	µl	µl	NOUN
elements-9604	39	15	of	of	ADP
elements-9604	39	16	cloning	clone	VERB
elements-9604	39	17	vector	vector	NOUN
elements-9604	39	18	pcep4	pcep4	NOUN
elements-9604	39	19	,	,	PUNCT
elements-9604	39	20	2	2	NUM
elements-9604	39	21	µl	µl	NOUN
elements-9604	39	22	of	of	ADP
elements-9604	39	23	cutsmart	cutsmart	ADJ
elements-9604	39	24	buffer	buffer	NOUN
elements-9604	39	25	from	from	ADP
elements-9604	39	26	new	new	PROPN
elements-9604	39	27	england	england	PROPN
elements-9604	39	28	biolabs	biolab	NOUN
elements-9604	39	29	,	,	PUNCT
elements-9604	39	30	1	1	NUM
elements-9604	39	31	µl	µl	ADP
elements-9604	39	32	of	of	ADP
elements-9604	39	33	kpni	kpni	NOUN
elements-9604	39	34	,	,	PUNCT
elements-9604	39	35	1	1	NUM
elements-9604	39	36	µl	µl	ADP
elements-9604	39	37	of	of	ADP
elements-9604	39	38	sapi	sapi	NOUN
elements-9604	39	39	,	,	PUNCT
elements-9604	39	40	and	and	CCONJ
elements-9604	39	41	15	15	NUM
elements-9604	39	42	µl	µl	NOUN
elements-9604	39	43	of	of	ADP
elements-9604	39	44	double	double	ADJ
elements-9604	39	45	distilled	distil	VERB
elements-9604	39	46	water	water	NOUN
elements-9604	39	47	.	.	PUNCT
elements-9604	40	1	the	the	DET
elements-9604	40	2	other	other	ADJ
elements-9604	40	3	two	two	NUM
elements-9604	40	4	samples	sample	NOUN
elements-9604	40	5	served	serve	VERB
elements-9604	40	6	as	as	ADP
elements-9604	40	7	controls	control	NOUN
elements-9604	40	8	.	.	PUNCT
elements-9604	41	1	control	control	NOUN
elements-9604	41	2	1	1	NUM
elements-9604	41	3	,	,	PUNCT
elements-9604	41	4	the	the	DET
elements-9604	41	5	reaction	reaction	NOUN
elements-9604	41	6	mix	mix	NOUN
elements-9604	41	7	for	for	ADP
elements-9604	41	8	the	the	DET
elements-9604	41	9	single	single	ADJ
elements-9604	41	10	digest	digest	NOUN
elements-9604	41	11	using	use	VERB
elements-9604	41	12	kpni	kpni	PROPN
elements-9604	41	13	,	,	PUNCT
elements-9604	41	14	consisted	consist	VERB
elements-9604	41	15	of	of	ADP
elements-9604	41	16	1	1	NUM
elements-9604	41	17	µl	µl	NOUN
elements-9604	41	18	of	of	ADP
elements-9604	41	19	cloning	clone	VERB
elements-9604	41	20	vector	vector	NOUN
elements-9604	41	21	pcep4	pcep4	NOUN
elements-9604	41	22	,	,	PUNCT
elements-9604	41	23	2	2	NUM
elements-9604	41	24	µl	µl	NOUN
elements-9604	41	25	of	of	ADP
elements-9604	41	26	cutsmart	cutsmart	ADJ
elements-9604	41	27	buffer	buffer	NOUN
elements-9604	41	28	from	from	ADP
elements-9604	41	29	new	new	PROPN
elements-9604	41	30	england	england	PROPN
elements-9604	41	31	biolabs	biolab	NOUN
elements-9604	41	32	,	,	PUNCT
elements-9604	41	33	1	1	NUM
elements-9604	41	34	µl	µl	ADP
elements-9604	41	35	of	of	ADP
elements-9604	41	36	kpni	kpni	NOUN
elements-9604	41	37	,	,	PUNCT
elements-9604	41	38	0	0	NUM
elements-9604	41	39	µl	µl	NOUN
elements-9604	41	40	of	of	ADP
elements-9604	41	41	sapi	sapi	NOUN
elements-9604	41	42	,	,	PUNCT
elements-9604	41	43	and	and	CCONJ
elements-9604	41	44	16	16	NUM
elements-9604	41	45	µl	µl	NOUN
elements-9604	41	46	of	of	ADP
elements-9604	41	47	double	double	ADJ
elements-9604	41	48	distilled	distil	VERB
elements-9604	41	49	water	water	NOUN
elements-9604	41	50	.	.	PUNCT
elements-9604	42	1	control	control	NOUN
elements-9604	42	2	2	2	NUM
elements-9604	42	3	,	,	PUNCT
elements-9604	42	4	the	the	DET
elements-9604	42	5	reaction	reaction	NOUN
elements-9604	42	6	mix	mix	NOUN
elements-9604	42	7	for	for	ADP
elements-9604	42	8	the	the	DET
elements-9604	42	9	single	single	ADJ
elements-9604	42	10	digest	digest	NOUN
elements-9604	42	11	using	use	VERB
elements-9604	42	12	sapi	sapi	NOUN
elements-9604	42	13	,	,	PUNCT
elements-9604	42	14	consisted	consist	VERB
elements-9604	42	15	of	of	ADP
elements-9604	42	16	1	1	NUM
elements-9604	42	17	µl	µl	NOUN
elements-9604	42	18	of	of	ADP
elements-9604	42	19	cloning	clone	VERB
elements-9604	42	20	vector	vector	NOUN
elements-9604	42	21	pcep4	pcep4	NOUN
elements-9604	42	22	,	,	PUNCT
elements-9604	42	23	2	2	NUM
elements-9604	42	24	µl	µl	NOUN
elements-9604	42	25	of	of	ADP
elements-9604	42	26	cutsmart	cutsmart	ADJ
elements-9604	42	27	buffer	buffer	NOUN
elements-9604	42	28	from	from	ADP
elements-9604	42	29	new	new	PROPN
elements-9604	42	30	england	england	PROPN
elements-9604	42	31	biolabs	biolabs	PROPN
elements-9604	42	32	,	,	PUNCT
elements-9604	42	33	0	0	NUM
elements-9604	42	34	µl	µl	NOUN
elements-9604	42	35	of	of	ADP
elements-9604	42	36	kpni	kpni	NOUN
elements-9604	42	37	,	,	PUNCT
elements-9604	42	38	1	1	NUM
elements-9604	42	39	µl	µl	ADP
elements-9604	42	40	of	of	ADP
elements-9604	42	41	72	72	NUM
elements-9604	42	42	elements	element	NOUN
elements-9604	42	43	:	:	PUNCT
elements-9604	42	44	:	:	PUNCT
elements-9604	42	45	spring	spring	NOUN
elements-9604	42	46	2017	2017	NUM
elements-9604	42	47	sapi	sapi	NOUN
elements-9604	42	48	,	,	PUNCT
elements-9604	42	49	and	and	CCONJ
elements-9604	42	50	16	16	NUM
elements-9604	42	51	µl	µl	NOUN
elements-9604	42	52	of	of	ADP
elements-9604	42	53	double	double	ADJ
elements-9604	42	54	distilled	distil	VERB
elements-9604	42	55	water	water	NOUN
elements-9604	42	56	.	.	PUNCT
elements-9604	43	1	dna	dna	PROPN
elements-9604	43	2	gel	gel	NOUN
elements-9604	43	3	electrophoresis	electrophoresis	NOUN
elements-9604	43	4	was	be	AUX
elements-9604	43	5	performed	perform	VERB
elements-9604	43	6	on	on	ADP
elements-9604	43	7	the	the	DET
elements-9604	43	8	sample	sample	NOUN
elements-9604	43	9	reaction	reaction	NOUN
elements-9604	43	10	mix	mix	NOUN
elements-9604	43	11	and	and	CCONJ
elements-9604	43	12	the	the	DET
elements-9604	43	13	reaction	reaction	NOUN
elements-9604	43	14	mixes	mix	VERB
elements-9604	43	15	of	of	ADP
elements-9604	43	16	the	the	DET
elements-9604	43	17	two	two	NUM
elements-9604	43	18	controls	control	NOUN
elements-9604	43	19	on	on	ADP
elements-9604	43	20	1	1	NUM
elements-9604	43	21	%	%	NOUN
elements-9604	43	22	agarose	agarose	NOUN
elements-9604	43	23	gel	gel	NOUN
elements-9604	43	24	for	for	ADP
elements-9604	43	25	1	1	NUM
elements-9604	43	26	hour	hour	NOUN
elements-9604	43	27	.	.	PUNCT
elements-9604	44	1	extraction	extraction	NOUN
elements-9604	44	2	of	of	ADP
elements-9604	44	3	mammalian	mammalian	ADJ
elements-9604	44	4	expression	expression	NOUN
elements-9604	44	5	vector	vector	NOUN
elements-9604	44	6	pcep4	pcep4	NOUN
elements-9604	44	7	double	double	ADV
elements-9604	44	8	digested	digest	VERB
elements-9604	44	9	with	with	ADP
elements-9604	44	10	kpni	kpni	NOUN
elements-9604	44	11	and	and	CCONJ
elements-9604	44	12	sapi	sapi	VERB
elements-9604	44	13	the	the	DET
elements-9604	44	14	dna	dna	PROPN
elements-9604	44	15	backbone	backbone	NOUN
elements-9604	44	16	vector	vector	NOUN
elements-9604	44	17	of	of	ADP
elements-9604	44	18	mammalian	mammalian	ADJ
elements-9604	44	19	expression	expression	NOUN
elements-9604	44	20	vector	vector	NOUN
elements-9604	44	21	pcep4	pcep4	NOUN
elements-9604	44	22	double	double	ADV
elements-9604	44	23	digested	digest	VERB
elements-9604	44	24	with	with	ADP
elements-9604	44	25	kpni	kpni	PROPN
elements-9604	44	26	and	and	CCONJ
elements-9604	44	27	sapi	sapi	NOUN
elements-9604	44	28	was	be	AUX
elements-9604	44	29	extracted	extract	VERB
elements-9604	44	30	from	from	ADP
elements-9604	44	31	the	the	DET
elements-9604	44	32	1	1	NUM
elements-9604	44	33	%	%	NOUN
elements-9604	44	34	agarose	agarose	NOUN
elements-9604	44	35	gel	gel	NOUN
elements-9604	44	36	using	use	VERB
elements-9604	44	37	the	the	DET
elements-9604	44	38	qiaquick	qiaquick	NOUN
elements-9604	44	39	gel	gel	NOUN
elements-9604	44	40	extraction	extraction	NOUN
elements-9604	44	41	kit	kit	NOUN
elements-9604	44	42	protocol	protocol	NOUN
elements-9604	44	43	by	by	ADP
elements-9604	44	44	qiagen	qiagen	PROPN
elements-9604	44	45	.	.	PUNCT
elements-9604	45	1	instead	instead	ADV
elements-9604	45	2	of	of	ADP
elements-9604	45	3	the	the	DET
elements-9604	45	4	recommended	recommend	VERB
elements-9604	45	5	50	50	NUM
elements-9604	45	6	µl	µl	NOUN
elements-9604	45	7	of	of	ADP
elements-9604	45	8	buffer	buffer	NOUN
elements-9604	45	9	eb	eb	PROPN
elements-9604	45	10	,	,	PUNCT
elements-9604	45	11	30	30	NUM
elements-9604	45	12	µl	µl	NOUN
elements-9604	45	13	of	of	ADP
elements-9604	45	14	double	double	ADJ
elements-9604	45	15	distilled	distil	VERB
elements-9604	45	16	water	water	NOUN
elements-9604	45	17	was	be	AUX
elements-9604	45	18	added	add	VERB
elements-9604	45	19	to	to	ADP
elements-9604	45	20	the	the	DET
elements-9604	45	21	qiaquick	qiaquick	NOUN
elements-9604	45	22	membrane	membrane	NOUN
elements-9604	45	23	column	column	NOUN
elements-9604	45	24	to	to	PART
elements-9604	45	25	elute	elute	VERB
elements-9604	45	26	dna	dna	NOUN
elements-9604	45	27	and	and	CCONJ
elements-9604	45	28	centrifuged	centrifuge	VERB
elements-9604	45	29	immediately	immediately	ADV
elements-9604	45	30	.	.	PUNCT
elements-9604	46	1	loading	load	VERB
elements-9604	46	2	dye	dye	NOUN
elements-9604	46	3	was	be	AUX
elements-9604	46	4	not	not	PART
elements-9604	46	5	added	add	VERB
elements-9604	46	6	to	to	ADP
elements-9604	46	7	purified	purified	ADJ
elements-9604	46	8	dna	dna	PROPN
elements-9604	46	9	.	.	PUNCT
elements-9604	47	1	ligation	ligation	NOUN
elements-9604	47	2	of	of	ADP
elements-9604	47	3	ll25x	ll25x	NOUN
elements-9604	47	4	into	into	ADP
elements-9604	47	5	backbone	backbone	NOUN
elements-9604	47	6	vector	vector	NOUN
elements-9604	47	7	pcep4	pcep4	NOUN
elements-9604	47	8	dna	dna	NOUN
elements-9604	47	9	gel	gel	NOUN
elements-9604	47	10	electrophoresis	electrophoresis	NOUN
elements-9604	47	11	of	of	ADP
elements-9604	47	12	2	2	NUM
elements-9604	47	13	µl	µl	NOUN
elements-9604	47	14	of	of	ADP
elements-9604	47	15	the	the	DET
elements-9604	47	16	double	double	ADJ
elements-9604	47	17	digested	digest	VERB
elements-9604	47	18	backbone	backbone	NOUN
elements-9604	47	19	vector	vector	NOUN
elements-9604	47	20	pcep4	pcep4	NOUN
elements-9604	47	21	and	and	CCONJ
elements-9604	47	22	2	2	NUM
elements-9604	47	23	µl	µl	NOUN
elements-9604	47	24	of	of	ADP
elements-9604	47	25	the	the	DET
elements-9604	47	26	double	double	ADJ
elements-9604	47	27	digested	digest	VERB
elements-9604	47	28	ll25x	ll25x	NOUN
elements-9604	47	29	pcr	pcr	PROPN
elements-9604	47	30	product	product	NOUN
elements-9604	47	31	was	be	AUX
elements-9604	47	32	performed	perform	VERB
elements-9604	47	33	on	on	ADP
elements-9604	47	34	1	1	NUM
elements-9604	47	35	%	%	NOUN
elements-9604	47	36	agarose	agarose	NOUN
elements-9604	47	37	gel	gel	NOUN
elements-9604	47	38	for	for	ADP
elements-9604	47	39	1	1	NUM
elements-9604	47	40	hour	hour	NOUN
elements-9604	47	41	.	.	PUNCT
elements-9604	48	1	based	base	VERB
elements-9604	48	2	on	on	ADP
elements-9604	48	3	the	the	DET
elements-9604	48	4	band	band	NOUN
elements-9604	48	5	intensities	intensity	NOUN
elements-9604	48	6	on	on	ADP
elements-9604	48	7	the	the	DET
elements-9604	48	8	gel	gel	NOUN
elements-9604	48	9	,	,	PUNCT
elements-9604	48	10	the	the	DET
elements-9604	48	11	volumes	volume	NOUN
elements-9604	48	12	for	for	ADP
elements-9604	48	13	the	the	DET
elements-9604	48	14	backbone	backbone	NOUN
elements-9604	48	15	and	and	CCONJ
elements-9604	48	16	insert	insert	ADJ
elements-9604	48	17	vectors	vector	NOUN
elements-9604	48	18	could	could	AUX
elements-9604	48	19	be	be	AUX
elements-9604	48	20	estimated	estimate	VERB
elements-9604	48	21	to	to	PART
elements-9604	48	22	achieve	achieve	VERB
elements-9604	48	23	a	a	DET
elements-9604	48	24	backbone	backbone	NOUN
elements-9604	48	25	-	-	PUNCT
elements-9604	48	26	to	to	ADP
elements-9604	48	27	-	-	PUNCT
elements-9604	48	28	insert	insert	VERB
elements-9604	48	29	ratio	ratio	NOUN
elements-9604	48	30	of	of	ADP
elements-9604	48	31	1:2	1:2	NUM
elements-9604	48	32	or	or	CCONJ
elements-9604	48	33	greater	great	ADJ
elements-9604	48	34	.	.	PUNCT
elements-9604	49	1	two	two	NUM
elements-9604	49	2	tubes	tube	NOUN
elements-9604	49	3	were	be	AUX
elements-9604	49	4	prepared	prepare	VERB
elements-9604	49	5	.	.	PUNCT
elements-9604	50	1	one	one	NUM
elements-9604	50	2	tube	tube	NOUN
elements-9604	50	3	was	be	AUX
elements-9604	50	4	for	for	ADP
elements-9604	50	5	ligation	ligation	NOUN
elements-9604	50	6	,	,	PUNCT
elements-9604	50	7	and	and	CCONJ
elements-9604	50	8	the	the	DET
elements-9604	50	9	other	other	ADJ
elements-9604	50	10	tube	tube	NOUN
elements-9604	50	11	served	serve	VERB
elements-9604	50	12	as	as	ADP
elements-9604	50	13	a	a	DET
elements-9604	50	14	control	control	NOUN
elements-9604	50	15	.	.	PUNCT
elements-9604	51	1	the	the	DET
elements-9604	51	2	reaction	reaction	NOUN
elements-9604	51	3	mixture	mixture	NOUN
elements-9604	51	4	for	for	ADP
elements-9604	51	5	ligation	ligation	NOUN
elements-9604	51	6	of	of	ADP
elements-9604	51	7	the	the	DET
elements-9604	51	8	double	double	ADJ
elements-9604	51	9	digested	digest	VERB
elements-9604	51	10	ll25x	ll25x	NOUN
elements-9604	51	11	pcr	pcr	PROPN
elements-9604	51	12	product	product	NOUN
elements-9604	51	13	into	into	ADP
elements-9604	51	14	backbone	backbone	NOUN
elements-9604	51	15	vector	vector	NOUN
elements-9604	51	16	pcep4	pcep4	NOUN
elements-9604	51	17	consisted	consist	VERB
elements-9604	51	18	of	of	ADP
elements-9604	51	19	2	2	NUM
elements-9604	51	20	µl	µl	NOUN
elements-9604	51	21	of	of	ADP
elements-9604	51	22	the	the	DET
elements-9604	51	23	backbone	backbone	NOUN
elements-9604	51	24	vector	vector	NOUN
elements-9604	51	25	(	(	PUNCT
elements-9604	51	26	kpni	kpni	NOUN
elements-9604	51	27	/	/	SYM
elements-9604	51	28	sapi	sapi	NOUN
elements-9604	51	29	-	-	PUNCT
elements-9604	51	30	digested	digest	VERB
elements-9604	51	31	pcep4	pcep4	NOUN
elements-9604	51	32	)	)	PUNCT
elements-9604	51	33	,	,	PUNCT
elements-9604	51	34	4	4	NUM
elements-9604	51	35	µl	µl	NOUN
elements-9604	51	36	of	of	ADP
elements-9604	51	37	the	the	DET
elements-9604	51	38	insert	insert	ADJ
elements-9604	51	39	vector	vector	NOUN
elements-9604	51	40	(	(	PUNCT
elements-9604	51	41	kpni	kpni	NOUN
elements-9604	51	42	/	/	SYM
elements-9604	51	43	sapi	sapi	NOUN
elements-9604	51	44	-	-	PUNCT
elements-9604	51	45	digested	digest	VERB
elements-9604	51	46	ll25x	ll25x	NOUN
elements-9604	51	47	)	)	PUNCT
elements-9604	51	48	,	,	PUNCT
elements-9604	51	49	2	2	NUM
elements-9604	51	50	µl	µl	NOUN
elements-9604	51	51	of	of	ADP
elements-9604	51	52	t3	t3	PROPN
elements-9604	51	53	dna	dna	PROPN
elements-9604	51	54	ligation	ligation	NOUN
elements-9604	51	55	buffer	buffer	NOUN
elements-9604	51	56	(	(	PUNCT
elements-9604	51	57	10x	10x	NUM
elements-9604	51	58	)	)	PUNCT
elements-9604	51	59	,	,	PUNCT
elements-9604	51	60	1	1	NUM
elements-9604	51	61	µl	µl	NOUN
elements-9604	51	62	of	of	ADP
elements-9604	51	63	t4	t4	PROPN
elements-9604	51	64	dna	dna	PROPN
elements-9604	51	65	ligase	ligase	PROPN
elements-9604	51	66	,	,	PUNCT
elements-9604	51	67	and	and	CCONJ
elements-9604	51	68	11	11	NUM
elements-9604	51	69	µl	µl	NOUN
elements-9604	51	70	of	of	ADP
elements-9604	51	71	sterile	sterile	ADJ
elements-9604	51	72	water	water	NOUN
elements-9604	51	73	.	.	PUNCT
elements-9604	52	1	the	the	DET
elements-9604	52	2	reaction	reaction	NOUN
elements-9604	52	3	mixture	mixture	NOUN
elements-9604	52	4	for	for	ADP
elements-9604	52	5	the	the	DET
elements-9604	52	6	control	control	NOUN
elements-9604	52	7	consisted	consist	VERB
elements-9604	52	8	of	of	ADP
elements-9604	52	9	2	2	NUM
elements-9604	52	10	µl	µl	NOUN
elements-9604	52	11	of	of	ADP
elements-9604	52	12	the	the	DET
elements-9604	52	13	backbone	backbone	NOUN
elements-9604	52	14	vector	vector	NOUN
elements-9604	52	15	(	(	PUNCT
elements-9604	52	16	kpni	kpni	NOUN
elements-9604	52	17	/	/	SYM
elements-9604	52	18	sapidigested	sapidigeste	VERB
elements-9604	52	19	pcep4	pcep4	NOUN
elements-9604	52	20	)	)	PUNCT
elements-9604	52	21	,	,	PUNCT
elements-9604	52	22	0	0	NUM
elements-9604	52	23	µl	µl	NOUN
elements-9604	52	24	of	of	ADP
elements-9604	52	25	the	the	DET
elements-9604	52	26	insert	insert	ADJ
elements-9604	52	27	vector	vector	NOUN
elements-9604	52	28	(	(	PUNCT
elements-9604	52	29	kpni	kpni	NOUN
elements-9604	52	30	/	/	SYM
elements-9604	52	31	sapidigested	sapidigeste	VERB
elements-9604	52	32	ll25x	ll25x	NOUN
elements-9604	52	33	)	)	PUNCT
elements-9604	52	34	,	,	PUNCT
elements-9604	52	35	2	2	NUM
elements-9604	52	36	µl	µl	NOUN
elements-9604	52	37	of	of	ADP
elements-9604	52	38	t3	t3	PROPN
elements-9604	52	39	dna	dna	PROPN
elements-9604	52	40	ligation	ligation	NOUN
elements-9604	52	41	buffer	buffer	NOUN
elements-9604	52	42	(	(	PUNCT
elements-9604	52	43	10x	10x	NUM
elements-9604	52	44	)	)	PUNCT
elements-9604	52	45	,	,	PUNCT
elements-9604	52	46	1	1	NUM
elements-9604	52	47	µl	µl	NOUN
elements-9604	52	48	of	of	ADP
elements-9604	52	49	t4	t4	PROPN
elements-9604	52	50	dna	dna	PROPN
elements-9604	52	51	ligase	ligase	PROPN
elements-9604	52	52	,	,	PUNCT
elements-9604	52	53	and	and	CCONJ
elements-9604	52	54	15	15	NUM
elements-9604	52	55	µl	µl	NOUN
elements-9604	52	56	of	of	ADP
elements-9604	52	57	sterile	sterile	ADJ
elements-9604	52	58	water	water	NOUN
elements-9604	52	59	.	.	PUNCT
elements-9604	53	1	the	the	DET
elements-9604	53	2	ligation	ligation	NOUN
elements-9604	53	3	reaction	reaction	NOUN
elements-9604	53	4	products	product	NOUN
elements-9604	53	5	were	be	AUX
elements-9604	53	6	incubated	incubate	VERB
elements-9604	53	7	overnight	overnight	ADV
elements-9604	53	8	at	at	ADP
elements-9604	53	9	room	room	NOUN
elements-9604	53	10	temperature	temperature	NOUN
elements-9604	53	11	.	.	PUNCT
elements-9604	54	1	transformation	transformation	NOUN
elements-9604	54	2	of	of	ADP
elements-9604	54	3	ligation	ligation	NOUN
elements-9604	54	4	reaction	reaction	NOUN
elements-9604	54	5	products	product	NOUN
elements-9604	54	6	into	into	ADP
elements-9604	54	7	e.	e.	PROPN
elements-9604	54	8	coli	coli	PROPN
elements-9604	54	9	top10f	top10f	ADJ
elements-9604	54	10	frozen	frozen	ADJ
elements-9604	54	11	electrocompetent	electrocompetent	ADJ
elements-9604	54	12	e.	e.	PROPN
elements-9604	54	13	coli	coli	PROPN
elements-9604	54	14	top10f	top10f	NOUN
elements-9604	54	15	’	'	PUNCT
elements-9604	54	16	was	be	AUX
elements-9604	54	17	removed	remove	VERB
elements-9604	54	18	from	from	ADP
elements-9604	54	19	a	a	DET
elements-9604	54	20	-80	-80	NUM
elements-9604	54	21	°	°	NOUN
elements-9604	54	22	c	c	NOUN
elements-9604	54	23	freezer	freezer	NOUN
elements-9604	54	24	and	and	CCONJ
elements-9604	54	25	thawed	thaw	VERB
elements-9604	54	26	on	on	ADP
elements-9604	54	27	ice	ice	NOUN
elements-9604	54	28	for	for	ADP
elements-9604	54	29	10	10	NUM
elements-9604	54	30	minutes	minute	NOUN
elements-9604	54	31	.	.	PUNCT
elements-9604	55	1	the	the	DET
elements-9604	55	2	e.	e.	PROPN
elements-9604	55	3	coli	coli	PROPN
elements-9604	55	4	was	be	AUX
elements-9604	55	5	aliquoted	aliquote	VERB
elements-9604	55	6	to	to	PART
elements-9604	55	7	obtain	obtain	VERB
elements-9604	55	8	50	50	NUM
elements-9604	55	9	µl	µl	NOUN
elements-9604	55	10	in	in	ADP
elements-9604	55	11	a	a	DET
elements-9604	55	12	tube	tube	NOUN
elements-9604	55	13	,	,	PUNCT
elements-9604	55	14	and	and	CCONJ
elements-9604	55	15	7	7	NUM
elements-9604	55	16	µl	µl	NOUN
elements-9604	55	17	of	of	ADP
elements-9604	55	18	ligation	ligation	NOUN
elements-9604	55	19	reaction	reaction	NOUN
elements-9604	55	20	was	be	AUX
elements-9604	55	21	added	add	VERB
elements-9604	55	22	to	to	ADP
elements-9604	55	23	it	it	PRON
elements-9604	55	24	.	.	PUNCT
elements-9604	56	1	the	the	DET
elements-9604	56	2	tip	tip	NOUN
elements-9604	56	3	of	of	ADP
elements-9604	56	4	the	the	DET
elements-9604	56	5	pipette	pipette	NOUN
elements-9604	56	6	was	be	AUX
elements-9604	56	7	swirled	swirl	VERB
elements-9604	56	8	in	in	ADP
elements-9604	56	9	the	the	DET
elements-9604	56	10	tube	tube	NOUN
elements-9604	56	11	to	to	PART
elements-9604	56	12	mix	mix	VERB
elements-9604	56	13	the	the	DET
elements-9604	56	14	solution	solution	NOUN
elements-9604	56	15	,	,	PUNCT
elements-9604	56	16	and	and	CCONJ
elements-9604	56	17	the	the	DET
elements-9604	56	18	tube	tube	NOUN
elements-9604	56	19	was	be	AUX
elements-9604	56	20	incubated	incubate	VERB
elements-9604	56	21	on	on	ADP
elements-9604	56	22	ice	ice	NOUN
elements-9604	56	23	for	for	ADP
elements-9604	56	24	30	30	NUM
elements-9604	56	25	minutes	minute	NOUN
elements-9604	56	26	.	.	PUNCT
elements-9604	57	1	the	the	DET
elements-9604	57	2	transformation	transformation	NOUN
elements-9604	57	3	tubes	tube	NOUN
elements-9604	57	4	were	be	AUX
elements-9604	57	5	heat	heat	NOUN
elements-9604	57	6	shocked	shock	VERB
elements-9604	57	7	at	at	ADP
elements-9604	57	8	42	42	NUM
elements-9604	57	9	°	°	ADP
elements-9604	57	10	c	c	NOUN
elements-9604	57	11	for	for	ADP
elements-9604	57	12	1	1	NUM
elements-9604	57	13	minute	minute	NOUN
elements-9604	57	14	and	and	CCONJ
elements-9604	57	15	put	put	VERB
elements-9604	57	16	on	on	ADP
elements-9604	57	17	ice	ice	NOUN
elements-9604	57	18	for	for	ADP
elements-9604	57	19	2	2	NUM
elements-9604	57	20	minutes	minute	NOUN
elements-9604	57	21	.	.	PUNCT
elements-9604	58	1	the	the	DET
elements-9604	58	2	heat	heat	NOUN
elements-9604	58	3	shock	shock	NOUN
elements-9604	58	4	allowed	allow	VERB
elements-9604	58	5	the	the	DET
elements-9604	58	6	bacterial	bacterial	ADJ
elements-9604	58	7	membranes	membrane	NOUN
elements-9604	58	8	to	to	PART
elements-9604	58	9	be	be	AUX
elements-9604	58	10	permeable	permeable	ADJ
elements-9604	58	11	enough	enough	ADV
elements-9604	58	12	for	for	ADP
elements-9604	58	13	vector	vector	NOUN
elements-9604	58	14	pcep4	pcep4	NOUN
elements-9604	58	15	containing	contain	VERB
elements-9604	58	16	the	the	DET
elements-9604	58	17	ll25x	ll25x	PROPN
elements-9604	58	18	dna	dna	PROPN
elements-9604	58	19	to	to	PART
elements-9604	58	20	enter	enter	VERB
elements-9604	58	21	the	the	DET
elements-9604	58	22	cells	cell	NOUN
elements-9604	58	23	.	.	PUNCT
elements-9604	59	1	afterwards	afterwards	ADV
elements-9604	59	2	,	,	PUNCT
elements-9604	59	3	1	1	NUM
elements-9604	59	4	ml	ml	NOUN
elements-9604	59	5	of	of	ADP
elements-9604	59	6	lb	lb	PRON
elements-9604	59	7	media	medium	NOUN
elements-9604	59	8	without	without	ADP
elements-9604	59	9	antibiotics	antibiotic	NOUN
elements-9604	59	10	was	be	AUX
elements-9604	59	11	added	add	VERB
elements-9604	59	12	to	to	ADP
elements-9604	59	13	the	the	DET
elements-9604	59	14	tube	tube	NOUN
elements-9604	59	15	and	and	CCONJ
elements-9604	59	16	cultured	cultured	ADJ
elements-9604	59	17	at	at	ADP
elements-9604	59	18	37	37	NUM
elements-9604	59	19	°	°	ADP
elements-9604	59	20	c	c	NOUN
elements-9604	59	21	for	for	ADP
elements-9604	59	22	45	45	NUM
elements-9604	59	23	minutes	minute	NOUN
elements-9604	59	24	in	in	ADP
elements-9604	59	25	a	a	DET
elements-9604	59	26	shaking	shake	VERB
elements-9604	59	27	incubator	incubator	NOUN
elements-9604	59	28	at	at	ADP
elements-9604	59	29	250	250	NUM
elements-9604	59	30	rpm	rpm	NOUN
elements-9604	59	31	.	.	PUNCT
elements-9604	60	1	the	the	DET
elements-9604	60	2	tube	tube	NOUN
elements-9604	60	3	was	be	AUX
elements-9604	60	4	centrifuged	centrifuge	VERB
elements-9604	60	5	at	at	ADP
elements-9604	60	6	9,000	9,000	NUM
elements-9604	60	7	rpm	rpm	NOUN
elements-9604	60	8	for	for	ADP
elements-9604	60	9	1	1	NUM
elements-9604	60	10	minute	minute	NOUN
elements-9604	60	11	,	,	PUNCT
elements-9604	60	12	and	and	CCONJ
elements-9604	60	13	all	all	DET
elements-9604	60	14	but	but	SCONJ
elements-9604	60	15	100	100	NUM
elements-9604	60	16	µl	µl	NOUN
elements-9604	60	17	of	of	ADP
elements-9604	60	18	the	the	DET
elements-9604	60	19	supernatant	supernatant	NOUN
elements-9604	60	20	was	be	AUX
elements-9604	60	21	pipetted	pipette	VERB
elements-9604	60	22	out	out	ADP
elements-9604	60	23	.	.	PUNCT
elements-9604	61	1	the	the	DET
elements-9604	61	2	remaining	remain	VERB
elements-9604	61	3	100	100	NUM
elements-9604	61	4	µl	µl	NOUN
elements-9604	61	5	of	of	ADP
elements-9604	61	6	supernatant	supernatant	NOUN
elements-9604	61	7	was	be	AUX
elements-9604	61	8	mixed	mix	VERB
elements-9604	61	9	with	with	ADP
elements-9604	61	10	the	the	DET
elements-9604	61	11	bacterial	bacterial	ADJ
elements-9604	61	12	pellet	pellet	NOUN
elements-9604	61	13	using	use	VERB
elements-9604	61	14	a	a	DET
elements-9604	61	15	pipette	pipette	NOUN
elements-9604	61	16	tip	tip	NOUN
elements-9604	61	17	.	.	PUNCT
elements-9604	62	1	all	all	PRON
elements-9604	62	2	of	of	ADP
elements-9604	62	3	the	the	DET
elements-9604	62	4	bacterial	bacterial	ADJ
elements-9604	62	5	mix	mix	NOUN
elements-9604	62	6	was	be	AUX
elements-9604	62	7	plated	plate	VERB
elements-9604	62	8	onto	onto	ADP
elements-9604	62	9	an	an	DET
elements-9604	62	10	lb	lb	NUM
elements-9604	62	11	agar	agar	NOUN
elements-9604	62	12	plate	plate	NOUN
elements-9604	62	13	containing	contain	VERB
elements-9604	62	14	ampicillin	ampicillin	NOUN
elements-9604	62	15	.	.	PUNCT
elements-9604	63	1	a	a	DET
elements-9604	63	2	background	background	NOUN
elements-9604	63	3	control	control	NOUN
elements-9604	63	4	of	of	ADP
elements-9604	63	5	cloning	clone	VERB
elements-9604	63	6	vector	vector	NOUN
elements-9604	63	7	pcep4	pcep4	NOUN
elements-9604	63	8	-	-	PUNCT
elements-9604	63	9	fc	fc	PROPN
elements-9604	63	10	-	-	PUNCT
elements-9604	63	11	ha	ha	INTJ
elements-9604	63	12	,	,	PUNCT
elements-9604	63	13	digested	digest	VERB
elements-9604	63	14	and	and	CCONJ
elements-9604	63	15	ligated	ligate	VERB
elements-9604	63	16	without	without	ADP
elements-9604	63	17	the	the	DET
elements-9604	63	18	ll25x	ll25x	PROPN
elements-9604	63	19	insert	insert	PROPN
elements-9604	63	20	vector	vector	NOUN
elements-9604	63	21	,	,	PUNCT
elements-9604	63	22	was	be	AUX
elements-9604	63	23	also	also	ADV
elements-9604	63	24	plated	plate	VERB
elements-9604	63	25	on	on	ADP
elements-9604	63	26	the	the	DET
elements-9604	63	27	selective	selective	ADJ
elements-9604	63	28	media	medium	NOUN
elements-9604	63	29	.	.	PUNCT
elements-9604	64	1	colony	colony	NOUN
elements-9604	64	2	screening	screening	NOUN
elements-9604	64	3	of	of	ADP
elements-9604	64	4	positive	positive	ADJ
elements-9604	64	5	clones	clone	NOUN
elements-9604	64	6	on	on	ADP
elements-9604	64	7	selective	selective	ADJ
elements-9604	64	8	media	medium	NOUN
elements-9604	64	9	growth	growth	NOUN
elements-9604	64	10	of	of	ADP
elements-9604	64	11	the	the	DET
elements-9604	64	12	bacterial	bacterial	ADJ
elements-9604	64	13	cells	cell	NOUN
elements-9604	64	14	transformed	transform	VERB
elements-9604	64	15	with	with	ADP
elements-9604	64	16	the	the	DET
elements-9604	64	17	pcep4/	pcep4/	PROPN
elements-9604	64	18	ll25x	ll25x	NOUN
elements-9604	64	19	ligation	ligation	NOUN
elements-9604	64	20	product	product	NOUN
elements-9604	64	21	was	be	AUX
elements-9604	64	22	observed	observe	VERB
elements-9604	64	23	on	on	ADP
elements-9604	64	24	the	the	DET
elements-9604	64	25	lb	lb	NOUN
elements-9604	64	26	agar	agar	NOUN
elements-9604	64	27	plate	plate	NOUN
elements-9604	64	28	containing	contain	VERB
elements-9604	64	29	ampicillin	ampicillin	NOUN
elements-9604	64	30	.	.	PUNCT
elements-9604	65	1	two	two	NUM
elements-9604	65	2	colonies	colony	NOUN
elements-9604	65	3	were	be	AUX
elements-9604	65	4	picked	pick	VERB
elements-9604	65	5	up	up	ADP
elements-9604	65	6	from	from	ADP
elements-9604	65	7	the	the	DET
elements-9604	65	8	lb	lb	NUM
elements-9604	65	9	agar	agar	NOUN
elements-9604	65	10	plate	plate	NOUN
elements-9604	65	11	containing	contain	VERB
elements-9604	65	12	ampicillin	ampicillin	NOUN
elements-9604	65	13	and	and	CCONJ
elements-9604	65	14	were	be	AUX
elements-9604	65	15	inoculated	inoculate	VERB
elements-9604	65	16	with	with	ADP
elements-9604	65	17	3	3	NUM
elements-9604	65	18	ml	ml	NOUN
elements-9604	65	19	of	of	ADP
elements-9604	65	20	lb	lb	DET
elements-9604	65	21	media	medium	NOUN
elements-9604	65	22	containing	contain	VERB
elements-9604	65	23	50	50	NUM
elements-9604	65	24	µg	µg	NOUN
elements-9604	65	25	/	/	SYM
elements-9604	65	26	ml	ml	NOUN
elements-9604	65	27	of	of	ADP
elements-9604	65	28	ampicillin	ampicillin	NOUN
elements-9604	65	29	in	in	ADP
elements-9604	65	30	a	a	DET
elements-9604	65	31	15	15	NUM
elements-9604	65	32	ml	ml	NOUN
elements-9604	65	33	culture	culture	NOUN
elements-9604	65	34	tube	tube	NOUN
elements-9604	65	35	.	.	PUNCT
elements-9604	66	1	the	the	DET
elements-9604	66	2	tube	tube	NOUN
elements-9604	66	3	was	be	AUX
elements-9604	66	4	cultured	cultured	ADJ
elements-9604	66	5	overnight	overnight	ADV
elements-9604	66	6	at	at	ADP
elements-9604	66	7	37	37	NUM
elements-9604	66	8	°	°	NOUN
elements-9604	66	9	c	c	NOUN
elements-9604	66	10	in	in	ADP
elements-9604	66	11	a	a	DET
elements-9604	66	12	shaking	shake	VERB
elements-9604	66	13	incubator	incubator	NOUN
elements-9604	66	14	at	at	ADP
elements-9604	66	15	250	250	NUM
elements-9604	66	16	rpm	rpm	NOUN
elements-9604	66	17	.	.	PUNCT
elements-9604	67	1	the	the	DET
elements-9604	67	2	day	day	NOUN
elements-9604	67	3	after	after	ADP
elements-9604	67	4	,	,	PUNCT
elements-9604	67	5	2	2	NUM
elements-9604	67	6	ml	ml	NOUN
elements-9604	67	7	of	of	ADP
elements-9604	67	8	bacteria	bacteria	NOUN
elements-9604	67	9	was	be	AUX
elements-9604	67	10	aliquoted	aliquote	VERB
elements-9604	67	11	into	into	ADP
elements-9604	67	12	a	a	DET
elements-9604	67	13	2	2	NUM
elements-9604	67	14	ml	ml	NOUN
elements-9604	67	15	eppendorf	eppendorf	PROPN
elements-9604	67	16	tube	tube	NOUN
elements-9604	67	17	and	and	CCONJ
elements-9604	67	18	centrifuged	centrifuge	VERB
elements-9604	67	19	at	at	ADP
elements-9604	67	20	13,000	13,000	NUM
elements-9604	67	21	rpm	rpm	NOUN
elements-9604	67	22	at	at	ADP
elements-9604	67	23	room	room	NOUN
elements-9604	67	24	temperature	temperature	NOUN
elements-9604	67	25	to	to	PART
elements-9604	67	26	pellet	pellet	VERB
elements-9604	67	27	down	down	ADP
elements-9604	67	28	the	the	DET
elements-9604	67	29	bacteria	bacteria	NOUN
elements-9604	67	30	.	.	PUNCT
elements-9604	68	1	the	the	DET
elements-9604	68	2	supernatant	supernatant	NOUN
elements-9604	68	3	was	be	AUX
elements-9604	68	4	discarded	discard	VERB
elements-9604	68	5	,	,	PUNCT
elements-9604	68	6	and	and	CCONJ
elements-9604	68	7	the	the	DET
elements-9604	68	8	pellet	pellet	NOUN
elements-9604	68	9	was	be	AUX
elements-9604	68	10	obtained	obtain	VERB
elements-9604	68	11	.	.	PUNCT
elements-9604	69	1	extracting	extract	VERB
elements-9604	69	2	the	the	DET
elements-9604	69	3	pcep4	pcep4	NOUN
elements-9604	69	4	/	/	SYM
elements-9604	69	5	ll25x	ll25x	PROPN
elements-9604	69	6	dna	dna	PROPN
elements-9604	69	7	from	from	ADP
elements-9604	69	8	the	the	DET
elements-9604	69	9	e.	e.	PROPN
elements-9604	69	10	coli	coli	NOUN
elements-9604	69	11	cells	cell	NOUN
elements-9604	69	12	via	via	ADP
elements-9604	69	13	miniprep	miniprep	PROPN
elements-9604	69	14	the	the	DET
elements-9604	69	15	qiaprep	qiaprep	PROPN
elements-9604	69	16	spin	spin	NOUN
elements-9604	69	17	miniprep	miniprep	NOUN
elements-9604	69	18	kit	kit	NOUN
elements-9604	69	19	protocol	protocol	NOUN
elements-9604	69	20	by	by	ADP
elements-9604	69	21	qiagen	qiagen	PROPN
elements-9604	69	22	was	be	AUX
elements-9604	69	23	followed	follow	VERB
elements-9604	69	24	to	to	PART
elements-9604	69	25	isolate	isolate	VERB
elements-9604	69	26	the	the	DET
elements-9604	69	27	pcep4	pcep4	NOUN
elements-9604	69	28	/	/	SYM
elements-9604	69	29	ll25x	ll25x	PROPN
elements-9604	69	30	dna	dna	PROPN
elements-9604	69	31	from	from	ADP
elements-9604	69	32	the	the	DET
elements-9604	69	33	e.	e.	PROPN
elements-9604	69	34	coli	coli	NOUN
elements-9604	69	35	cells	cell	NOUN
elements-9604	69	36	.	.	PUNCT
elements-9604	70	1	the	the	DET
elements-9604	70	2	recommended	recommend	VERB
elements-9604	70	3	step	step	NOUN
elements-9604	70	4	involving	involve	VERB
elements-9604	70	5	washing	wash	VERB
elements-9604	70	6	the	the	DET
elements-9604	70	7	qiaprep	qiaprep	PROPN
elements-9604	70	8	spin	spin	NOUN
elements-9604	70	9	column	column	NOUN
elements-9604	70	10	with	with	ADP
elements-9604	70	11	0.5	0.5	NUM
elements-9604	70	12	ml	ml	NOUN
elements-9604	70	13	of	of	ADP
elements-9604	70	14	buffer	buffer	NOUN
elements-9604	70	15	pb	pb	ADP
elements-9604	70	16	was	be	AUX
elements-9604	70	17	omitted	omit	VERB
elements-9604	70	18	because	because	SCONJ
elements-9604	70	19	enda+	enda+	ADJ
elements-9604	70	20	strains	strain	NOUN
elements-9604	70	21	or	or	CCONJ
elements-9604	70	22	other	other	ADJ
elements-9604	70	23	bacterial	bacterial	ADJ
elements-9604	70	24	strains	strain	NOUN
elements-9604	70	25	with	with	ADP
elements-9604	70	26	high	high	ADJ
elements-9604	70	27	nuclease	nuclease	NOUN
elements-9604	70	28	activity	activity	NOUN
elements-9604	70	29	or	or	CCONJ
elements-9604	70	30	carbohydrate	carbohydrate	NOUN
elements-9604	70	31	content	content	NOUN
elements-9604	70	32	were	be	AUX
elements-9604	70	33	not	not	PART
elements-9604	70	34	used	use	VERB
elements-9604	70	35	.	.	PUNCT
elements-9604	71	1	none	none	NOUN
elements-9604	71	2	of	of	ADP
elements-9604	71	3	the	the	DET
elements-9604	71	4	optional	optional	ADJ
elements-9604	71	5	steps	step	NOUN
elements-9604	71	6	were	be	AUX
elements-9604	71	7	taken	take	VERB
elements-9604	71	8	,	,	PUNCT
elements-9604	71	9	such	such	ADJ
elements-9604	71	10	as	as	ADP
elements-9604	71	11	adding	add	VERB
elements-9604	71	12	lyseblue	lyseblue	ADJ
elements-9604	71	13	reagent	reagent	NOUN
elements-9604	71	14	to	to	PART
elements-9604	71	15	buffer	buffer	VERB
elements-9604	71	16	p1	p1	NOUN
elements-9604	71	17	.	.	PUNCT
elements-9604	72	1	the	the	DET
elements-9604	72	2	dna	dna	PROPN
elements-9604	72	3	concentration	concentration	NOUN
elements-9604	72	4	,	,	PUNCT
elements-9604	72	5	determined	determine	VERB
elements-9604	72	6	by	by	ADP
elements-9604	72	7	using	use	VERB
elements-9604	72	8	nanodrop	nanodrop	NOUN
elements-9604	72	9	,	,	PUNCT
elements-9604	72	10	was	be	AUX
elements-9604	72	11	120.2	120.2	NUM
elements-9604	72	12	ng/µl	ng/µl	NUM
elements-9604	72	13	.	.	PUNCT
elements-9604	73	1	determination	determination	NOUN
elements-9604	73	2	of	of	ADP
elements-9604	73	3	a	a	DET
elements-9604	73	4	positive	positive	ADJ
elements-9604	73	5	clone	clone	NOUN
elements-9604	73	6	by	by	ADP
elements-9604	73	7	restriction	restriction	NOUN
elements-9604	73	8	analysis	analysis	NOUN
elements-9604	73	9	one	one	NUM
elements-9604	73	10	reaction	reaction	NOUN
elements-9604	73	11	mix	mix	NOUN
elements-9604	73	12	contained	contain	VERB
elements-9604	73	13	the	the	DET
elements-9604	73	14	dna	dna	PROPN
elements-9604	73	15	template	template	NOUN
elements-9604	73	16	,	,	PUNCT
elements-9604	73	17	and	and	CCONJ
elements-9604	73	18	one	one	NUM
elements-9604	73	19	control	control	NOUN
elements-9604	73	20	tube	tube	NOUN
elements-9604	73	21	contained	contain	VERB
elements-9604	73	22	the	the	DET
elements-9604	73	23	cloning	cloning	NOUN
elements-9604	73	24	vector	vector	NOUN
elements-9604	73	25	pcep4	pcep4	NOUN
elements-9604	73	26	.	.	PUNCT
elements-9604	74	1	the	the	DET
elements-9604	74	2	reaction	reaction	NOUN
elements-9604	74	3	tube	tube	NOUN
elements-9604	74	4	consisted	consist	VERB
elements-9604	74	5	of	of	ADP
elements-9604	74	6	3	3	NUM
elements-9604	74	7	µl	µl	NOUN
elements-9604	74	8	of	of	ADP
elements-9604	74	9	mini	mini	NOUN
elements-9604	74	10	-	-	NOUN
elements-9604	74	11	prep	prep	ADJ
elements-9604	74	12	dna	dna	NOUN
elements-9604	74	13	,	,	PUNCT
elements-9604	74	14	2	2	NUM
elements-9604	74	15	µl	µl	NOUN
elements-9604	74	16	of	of	ADP
elements-9604	74	17	cutsmart	cutsmart	ADJ
elements-9604	74	18	buffer	buffer	NOUN
elements-9604	74	19	from	from	ADP
elements-9604	74	20	new	new	PROPN
elements-9604	74	21	england	england	PROPN
elements-9604	74	22	biolabs	biolab	NOUN
elements-9604	74	23	,	,	PUNCT
elements-9604	74	24	1	1	NUM
elements-9604	74	25	µl	µl	NOUN
elements-9604	74	26	of	of	ADP
elements-9604	74	27	kpnihf	kpnihf	PROPN
elements-9604	74	28	/	/	SYM
elements-9604	74	29	sapi	sapi	PROPN
elements-9604	74	30	,	,	PUNCT
elements-9604	74	31	1	1	NUM
elements-9604	74	32	µl	µl	ADP
elements-9604	74	33	of	of	ADP
elements-9604	74	34	kpni	kpni	NOUN
elements-9604	74	35	-	-	PUNCT
elements-9604	74	36	hf	hf	NOUN
elements-9604	74	37	/	/	SYM
elements-9604	74	38	bamhi	bamhi	NOUN
elements-9604	74	39	-	-	PUNCT
elements-9604	74	40	hf	hf	NOUN
elements-9604	74	41	,	,	PUNCT
elements-9604	74	42	and	and	CCONJ
elements-9604	74	43	13	13	NUM
elements-9604	74	44	µl	µl	NOUN
elements-9604	74	45	of	of	ADP
elements-9604	74	46	sterile	sterile	ADJ
elements-9604	74	47	water	water	NOUN
elements-9604	74	48	.	.	PUNCT
elements-9604	75	1	the	the	DET
elements-9604	75	2	control	control	NOUN
elements-9604	75	3	tube	tube	NOUN
elements-9604	75	4	consisted	consist	VERB
elements-9604	75	5	of	of	ADP
elements-9604	75	6	3	3	NUM
elements-9604	75	7	µl	µl	NOUN
elements-9604	75	8	of	of	ADP
elements-9604	75	9	cloning	clone	VERB
elements-9604	75	10	vector	vector	NOUN
elements-9604	75	11	pcep4	pcep4	NOUN
elements-9604	75	12	,	,	PUNCT
elements-9604	75	13	2	2	NUM
elements-9604	75	14	µl	µl	NOUN
elements-9604	75	15	of	of	ADP
elements-9604	75	16	cutsmart	cutsmart	ADJ
elements-9604	75	17	buffer	buffer	NOUN
elements-9604	75	18	from	from	ADP
elements-9604	75	19	new	new	PROPN
elements-9604	75	20	england	england	PROPN
elements-9604	75	21	biolabs	biolab	NOUN
elements-9604	75	22	,	,	PUNCT
elements-9604	75	23	1	1	NUM
elements-9604	75	24	µl	µl	ADP
elements-9604	75	25	of	of	ADP
elements-9604	75	26	kpni	kpni	NOUN
elements-9604	75	27	-	-	PUNCT
elements-9604	75	28	hf	hf	NOUN
elements-9604	75	29	/	/	SYM
elements-9604	75	30	sapi	sapi	NOUN
elements-9604	75	31	,	,	PUNCT
elements-9604	75	32	1	1	NUM
elements-9604	75	33	µl	µl	ADP
elements-9604	75	34	of	of	ADP
elements-9604	75	35	kpni	kpni	NOUN
elements-9604	75	36	-	-	PUNCT
elements-9604	75	37	hf	hf	NOUN
elements-9604	75	38	/	/	SYM
elements-9604	75	39	bamhi	bamhi	NOUN
elements-9604	75	40	-	-	PUNCT
elements-9604	75	41	hf	hf	NOUN
elements-9604	75	42	,	,	PUNCT
elements-9604	75	43	and	and	CCONJ
elements-9604	75	44	13	13	NUM
elements-9604	75	45	µl	µl	NOUN
elements-9604	75	46	of	of	ADP
elements-9604	75	47	sterile	sterile	ADJ
elements-9604	75	48	water	water	NOUN
elements-9604	75	49	.	.	PUNCT
elements-9604	76	1	both	both	DET
elements-9604	76	2	tubes	tube	NOUN
elements-9604	76	3	had	have	VERB
elements-9604	76	4	a	a	DET
elements-9604	76	5	total	total	ADJ
elements-9604	76	6	volume	volume	NOUN
elements-9604	76	7	of	of	ADP
elements-9604	76	8	73	73	NUM
elements-9604	76	9	the	the	DET
elements-9604	76	10	road	road	NOUN
elements-9604	76	11	to	to	ADP
elements-9604	76	12	a	a	DET
elements-9604	76	13	cure	cure	NOUN
elements-9604	76	14	20	20	NUM
elements-9604	76	15	µl	µl	NOUN
elements-9604	76	16	.	.	PUNCT
elements-9604	77	1	the	the	DET
elements-9604	77	2	tubes	tube	NOUN
elements-9604	77	3	were	be	AUX
elements-9604	77	4	incubated	incubate	VERB
elements-9604	77	5	at	at	ADP
elements-9604	77	6	37	37	NUM
elements-9604	77	7	°	°	ADP
elements-9604	77	8	c	c	NOUN
elements-9604	77	9	for	for	ADP
elements-9604	77	10	1	1	NUM
elements-9604	77	11	hour	hour	NOUN
elements-9604	77	12	,	,	PUNCT
elements-9604	77	13	and	and	CCONJ
elements-9604	77	14	the	the	DET
elements-9604	77	15	products	product	NOUN
elements-9604	77	16	of	of	ADP
elements-9604	77	17	the	the	DET
elements-9604	77	18	double	double	ADJ
elements-9604	77	19	digest	digest	NOUN
elements-9604	77	20	were	be	AUX
elements-9604	77	21	analyzed	analyze	VERB
elements-9604	77	22	on	on	ADP
elements-9604	77	23	a	a	DET
elements-9604	77	24	1	1	NUM
elements-9604	77	25	%	%	NOUN
elements-9604	77	26	agarose	agarose	NOUN
elements-9604	77	27	gel	gel	NOUN
elements-9604	77	28	.	.	PUNCT
elements-9604	78	1	preparation	preparation	NOUN
elements-9604	78	2	of	of	ADP
elements-9604	78	3	samples	sample	NOUN
elements-9604	78	4	for	for	ADP
elements-9604	78	5	dna	dna	NOUN
elements-9604	78	6	sequencing	sequence	VERB
elements-9604	78	7	after	after	ADP
elements-9604	78	8	dna	dna	PROPN
elements-9604	78	9	mini	mini	NOUN
elements-9604	78	10	-	-	NOUN
elements-9604	78	11	prep	prep	NOUN
elements-9604	78	12	,	,	PUNCT
elements-9604	78	13	samples	sample	NOUN
elements-9604	78	14	were	be	AUX
elements-9604	78	15	prepared	prepare	VERB
elements-9604	78	16	for	for	ADP
elements-9604	78	17	dna	dna	NOUN
elements-9604	78	18	sequencing	sequencing	NOUN
elements-9604	78	19	.	.	PUNCT
elements-9604	79	1	recommendations	recommendation	NOUN
elements-9604	79	2	for	for	ADP
elements-9604	79	3	dna	dna	NOUN
elements-9604	79	4	sequencing	sequence	VERB
elements-9604	79	5	from	from	ADP
elements-9604	79	6	eton	eton	PROPN
elements-9604	79	7	bioscience	bioscience	NOUN
elements-9604	79	8	were	be	AUX
elements-9604	79	9	followed	follow	VERB
elements-9604	79	10	.	.	PUNCT
elements-9604	80	1	for	for	ADP
elements-9604	80	2	successful	successful	ADJ
elements-9604	80	3	dna	dna	PROPN
elements-9604	80	4	sequencing	sequencing	NOUN
elements-9604	80	5	,	,	PUNCT
elements-9604	80	6	the	the	DET
elements-9604	80	7	reaction	reaction	NOUN
elements-9604	80	8	mix	mix	NOUN
elements-9604	80	9	consisted	consist	VERB
elements-9604	80	10	of	of	ADP
elements-9604	80	11	8	8	NUM
elements-9604	80	12	µl	µl	NOUN
elements-9604	80	13	of	of	ADP
elements-9604	80	14	the	the	DET
elements-9604	80	15	ll25x	ll25x	PROPN
elements-9604	80	16	pcr	pcr	PROPN
elements-9604	80	17	product	product	NOUN
elements-9604	80	18	,	,	PUNCT
elements-9604	80	19	8	8	NUM
elements-9604	80	20	µl	µl	NOUN
elements-9604	80	21	of	of	ADP
elements-9604	80	22	plasmid	plasmid	NOUN
elements-9604	80	23	that	that	PRON
elements-9604	80	24	was	be	AUX
elements-9604	80	25	less	less	ADJ
elements-9604	80	26	than	than	ADP
elements-9604	80	27	6	6	NUM
elements-9604	80	28	kb	kb	NOUN
elements-9604	80	29	and	and	CCONJ
elements-9604	80	30	had	have	VERB
elements-9604	80	31	a	a	DET
elements-9604	80	32	concentration	concentration	NOUN
elements-9604	80	33	of	of	ADP
elements-9604	80	34	50	50	NUM
elements-9604	80	35	-	-	SYM
elements-9604	80	36	150	150	NUM
elements-9604	80	37	ng/µl	ng/µl	NUM
elements-9604	80	38	(	(	PUNCT
elements-9604	80	39	obtained	obtain	VERB
elements-9604	80	40	by	by	ADP
elements-9604	80	41	dna	dna	PROPN
elements-9604	80	42	mini	mini	NOUN
elements-9604	80	43	-	-	NOUN
elements-9604	80	44	prep	prep	ADJ
elements-9604	80	45	and	and	CCONJ
elements-9604	80	46	determined	determine	VERB
elements-9604	80	47	by	by	ADP
elements-9604	80	48	nanodrop	nanodrop	NOUN
elements-9604	80	49	)	)	PUNCT
elements-9604	80	50	,	,	PUNCT
elements-9604	80	51	and	and	CCONJ
elements-9604	80	52	5	5	NUM
elements-9604	80	53	µl	µl	NOUN
elements-9604	80	54	of	of	ADP
elements-9604	80	55	primer	primer	NOUN
elements-9604	80	56	that	that	PRON
elements-9604	80	57	had	have	VERB
elements-9604	80	58	a	a	DET
elements-9604	80	59	concentration	concentration	NOUN
elements-9604	80	60	of	of	ADP
elements-9604	80	61	5	5	NUM
elements-9604	80	62	µm	µm	NOUN
elements-9604	80	63	.	.	PUNCT
elements-9604	81	1	the	the	DET
elements-9604	81	2	reaction	reaction	NOUN
elements-9604	81	3	mix	mix	NOUN
elements-9604	81	4	was	be	AUX
elements-9604	81	5	sent	send	VERB
elements-9604	81	6	to	to	ADP
elements-9604	81	7	eton	eton	PROPN
elements-9604	81	8	bioscience	bioscience	PROPN
elements-9604	81	9	for	for	ADP
elements-9604	81	10	dna	dna	NOUN
elements-9604	81	11	sequencing	sequencing	NOUN
elements-9604	81	12	.	.	PUNCT
elements-9604	82	1	sequence	sequence	NOUN
elements-9604	82	2	analysis	analysis	NOUN
elements-9604	82	3	of	of	ADP
elements-9604	82	4	ll25x	ll25x	NOUN
elements-9604	82	5	once	once	SCONJ
elements-9604	82	6	the	the	DET
elements-9604	82	7	dna	dna	PROPN
elements-9604	82	8	sequence	sequence	NOUN
elements-9604	82	9	results	result	NOUN
elements-9604	82	10	were	be	AUX
elements-9604	82	11	received	receive	VERB
elements-9604	82	12	from	from	ADP
elements-9604	82	13	eton	eton	PROPN
elements-9604	82	14	bioscience	bioscience	PROPN
elements-9604	82	15	,	,	PUNCT
elements-9604	82	16	the	the	DET
elements-9604	82	17	software	software	NOUN
elements-9604	82	18	geneious	geneious	ADJ
elements-9604	82	19	was	be	AUX
elements-9604	82	20	used	use	VERB
elements-9604	82	21	to	to	PART
elements-9604	82	22	analyze	analyze	VERB
elements-9604	82	23	the	the	DET
elements-9604	82	24	sequence	sequence	NOUN
elements-9604	82	25	of	of	ADP
elements-9604	82	26	ll25x	ll25x	PROPN
elements-9604	82	27	.	.	PUNCT
elements-9604	83	1	bacteria	bacteria	NOUN
elements-9604	83	2	culture	culture	NOUN
elements-9604	83	3	in	in	ADP
elements-9604	83	4	aqueous	aqueous	ADJ
elements-9604	83	5	lb	lb	NOUN
elements-9604	83	6	media	medium	NOUN
elements-9604	83	7	with	with	ADP
elements-9604	83	8	ampicillin	ampicillin	NOUN
elements-9604	83	9	,	,	PUNCT
elements-9604	83	10	followed	follow	VERB
elements-9604	83	11	by	by	ADP
elements-9604	83	12	dna	dna	PROPN
elements-9604	83	13	maxi	maxi	NOUN
elements-9604	83	14	-	-	PUNCT
elements-9604	83	15	prep	prep	NOUN
elements-9604	83	16	a	a	DET
elements-9604	83	17	selected	select	VERB
elements-9604	83	18	positive	positive	ADJ
elements-9604	83	19	clone	clone	NOUN
elements-9604	83	20	was	be	AUX
elements-9604	83	21	cultured	cultured	ADJ
elements-9604	83	22	for	for	ADP
elements-9604	83	23	8	8	NUM
elements-9604	83	24	hours	hour	NOUN
elements-9604	83	25	in	in	ADP
elements-9604	83	26	a	a	DET
elements-9604	83	27	culture	culture	NOUN
elements-9604	83	28	tube	tube	NOUN
elements-9604	83	29	containing	contain	VERB
elements-9604	83	30	3	3	NUM
elements-9604	83	31	ml	ml	NOUN
elements-9604	83	32	of	of	ADP
elements-9604	83	33	lb	lb	PRON
elements-9604	83	34	/	/	SYM
elements-9604	83	35	ampicillin	ampicillin	NOUN
elements-9604	83	36	media	medium	NOUN
elements-9604	83	37	.	.	PUNCT
elements-9604	84	1	from	from	ADP
elements-9604	84	2	the	the	DET
elements-9604	84	3	tube	tube	NOUN
elements-9604	84	4	,	,	PUNCT
elements-9604	84	5	100	100	NUM
elements-9604	84	6	µl	µl	NOUN
elements-9604	84	7	of	of	ADP
elements-9604	84	8	the	the	DET
elements-9604	84	9	culture	culture	NOUN
elements-9604	84	10	was	be	AUX
elements-9604	84	11	transferred	transfer	VERB
elements-9604	84	12	to	to	ADP
elements-9604	84	13	a	a	DET
elements-9604	84	14	flask	flask	NOUN
elements-9604	84	15	with	with	ADP
elements-9604	84	16	500	500	NUM
elements-9604	84	17	ml	ml	NOUN
elements-9604	84	18	of	of	ADP
elements-9604	84	19	lb	lb	PRON
elements-9604	84	20	/	/	SYM
elements-9604	84	21	ampicillin	ampicillin	NOUN
elements-9604	84	22	and	and	CCONJ
elements-9604	84	23	cultured	cultured	ADJ
elements-9604	84	24	overnight	overnight	ADV
elements-9604	84	25	.	.	PUNCT
elements-9604	85	1	the	the	DET
elements-9604	85	2	culture	culture	NOUN
elements-9604	85	3	was	be	AUX
elements-9604	85	4	transferred	transfer	VERB
elements-9604	85	5	into	into	ADP
elements-9604	85	6	centrifugation	centrifugation	NOUN
elements-9604	85	7	tubes	tube	NOUN
elements-9604	85	8	the	the	DET
elements-9604	85	9	next	next	ADJ
elements-9604	85	10	day	day	NOUN
elements-9604	85	11	.	.	PUNCT
elements-9604	86	1	the	the	DET
elements-9604	86	2	centrifuge	centrifuge	NOUN
elements-9604	86	3	was	be	AUX
elements-9604	86	4	set	set	VERB
elements-9604	86	5	at	at	ADP
elements-9604	86	6	7,000	7,000	NUM
elements-9604	86	7	rpm	rpm	NOUN
elements-9604	86	8	for	for	ADP
elements-9604	86	9	15	15	NUM
elements-9604	86	10	minutes	minute	NOUN
elements-9604	86	11	to	to	PART
elements-9604	86	12	pellet	pellet	VERB
elements-9604	86	13	the	the	DET
elements-9604	86	14	cells	cell	NOUN
elements-9604	86	15	.	.	PUNCT
elements-9604	87	1	the	the	DET
elements-9604	87	2	supernatant	supernatant	NOUN
elements-9604	87	3	was	be	AUX
elements-9604	87	4	discarded	discard	VERB
elements-9604	87	5	,	,	PUNCT
elements-9604	87	6	and	and	CCONJ
elements-9604	87	7	the	the	DET
elements-9604	87	8	pellet	pellet	NOUN
elements-9604	87	9	was	be	AUX
elements-9604	87	10	obtained	obtain	VERB
elements-9604	87	11	.	.	PUNCT
elements-9604	88	1	the	the	DET
elements-9604	88	2	purelink	purelink	ADJ
elements-9604	88	3	hipure	hipure	NOUN
elements-9604	88	4	plasmid	plasmid	VERB
elements-9604	88	5	dna	dna	PROPN
elements-9604	88	6	purification	purification	NOUN
elements-9604	88	7	kit	kit	NOUN
elements-9604	88	8	maxiprep	maxiprep	NOUN
elements-9604	88	9	procedure	procedure	NOUN
elements-9604	88	10	by	by	ADP
elements-9604	88	11	invitrogen	invitrogen	PROPN
elements-9604	88	12	was	be	AUX
elements-9604	88	13	followed	follow	VERB
elements-9604	88	14	to	to	PART
elements-9604	88	15	extract	extract	VERB
elements-9604	88	16	dna	dna	NOUN
elements-9604	88	17	from	from	ADP
elements-9604	88	18	the	the	DET
elements-9604	88	19	pellet	pellet	NOUN
elements-9604	88	20	.	.	PUNCT
elements-9604	89	1	the	the	DET
elements-9604	89	2	supernatant	supernatant	NOUN
elements-9604	89	3	was	be	AUX
elements-9604	89	4	removed	remove	VERB
elements-9604	89	5	,	,	PUNCT
elements-9604	89	6	and	and	CCONJ
elements-9604	89	7	the	the	DET
elements-9604	89	8	pellet	pellet	NOUN
elements-9604	89	9	was	be	AUX
elements-9604	89	10	air	air	NOUN
elements-9604	89	11	-	-	PUNCT
elements-9604	89	12	dried	dry	VERB
elements-9604	89	13	for	for	ADP
elements-9604	89	14	10	10	NUM
elements-9604	89	15	minutes	minute	NOUN
elements-9604	89	16	and	and	CCONJ
elements-9604	89	17	resuspended	resuspend	VERB
elements-9604	89	18	in	in	ADP
elements-9604	89	19	100	100	NUM
elements-9604	89	20	µl	µl	ADP
elements-9604	89	21	of	of	ADP
elements-9604	89	22	double	double	ADJ
elements-9604	89	23	distilled	distil	VERB
elements-9604	89	24	water	water	NOUN
elements-9604	89	25	,	,	PUNCT
elements-9604	89	26	rather	rather	ADV
elements-9604	89	27	than	than	ADP
elements-9604	89	28	the	the	DET
elements-9604	89	29	recommended	recommend	VERB
elements-9604	89	30	500	500	NUM
elements-9604	89	31	µl	µl	NOUN
elements-9604	89	32	of	of	ADP
elements-9604	89	33	te	te	ADP
elements-9604	89	34	buffer	buffer	NOUN
elements-9604	89	35	(	(	PUNCT
elements-9604	89	36	te	te	PROPN
elements-9604	89	37	)	)	PUNCT
elements-9604	89	38	.	.	PUNCT
elements-9604	90	1	initiation	initiation	NOUN
elements-9604	90	2	to	to	ADP
elements-9604	90	3	tissue	tissue	NOUN
elements-9604	90	4	culture	culture	NOUN
elements-9604	90	5	human	human	ADJ
elements-9604	90	6	embryonic	embryonic	ADJ
elements-9604	90	7	kidney	kidney	NOUN
elements-9604	90	8	293	293	NUM
elements-9604	90	9	cells	cell	NOUN
elements-9604	90	10	(	(	PUNCT
elements-9604	90	11	hek293	hek293	PROPN
elements-9604	90	12	)	)	PUNCT
elements-9604	90	13	of	of	ADP
elements-9604	90	14	the	the	DET
elements-9604	90	15	293	293	NUM
elements-9604	90	16	t	t	NOUN
elements-9604	90	17	cell	cell	NOUN
elements-9604	90	18	line	line	NOUN
elements-9604	90	19	were	be	AUX
elements-9604	90	20	used	use	VERB
elements-9604	90	21	.	.	PUNCT
elements-9604	91	1	for	for	ADP
elements-9604	91	2	storage	storage	NOUN
elements-9604	91	3	,	,	PUNCT
elements-9604	91	4	cells	cell	NOUN
elements-9604	91	5	were	be	AUX
elements-9604	91	6	transferred	transfer	VERB
elements-9604	91	7	into	into	ADP
elements-9604	91	8	a	a	DET
elements-9604	91	9	new	new	ADJ
elements-9604	91	10	t75	t75	NOUN
elements-9604	91	11	flask	flask	NOUN
elements-9604	91	12	containing	contain	VERB
elements-9604	91	13	500	500	NUM
elements-9604	91	14	ml	ml	NOUN
elements-9604	91	15	of	of	ADP
elements-9604	91	16	medium	medium	NOUN
elements-9604	91	17	consisting	consist	VERB
elements-9604	91	18	of	of	ADP
elements-9604	91	19	440	440	NUM
elements-9604	91	20	ml	ml	NOUN
elements-9604	91	21	of	of	ADP
elements-9604	91	22	rpmi	rpmi	NOUN
elements-9604	91	23	+	+	CCONJ
elements-9604	91	24	50	50	NUM
elements-9604	91	25	ml	ml	NOUN
elements-9604	91	26	of	of	ADP
elements-9604	91	27	10	10	NUM
elements-9604	91	28	%	%	NOUN
elements-9604	91	29	fbs	fb	NOUN
elements-9604	91	30	,	,	PUNCT
elements-9604	91	31	5	5	NUM
elements-9604	91	32	ml	ml	NOUN
elements-9604	91	33	of	of	ADP
elements-9604	91	34	supplemental	supplemental	ADJ
elements-9604	91	35	glutamine	glutamine	NOUN
elements-9604	91	36	200	200	NUM
elements-9604	91	37	mm	mm	NOUN
elements-9604	91	38	l	l	NOUN
elements-9604	91	39	-	-	NOUN
elements-9604	91	40	glu	glu	ADJ
elements-9604	91	41	,	,	PUNCT
elements-9604	91	42	and	and	CCONJ
elements-9604	91	43	5	5	NUM
elements-9604	91	44	ml	ml	NOUN
elements-9604	91	45	of	of	ADP
elements-9604	91	46	1x	1x	NUM
elements-9604	91	47	penicillin	penicillin	NOUN
elements-9604	91	48	and	and	CCONJ
elements-9604	91	49	1x	1x	NUM
elements-9604	91	50	streptomycin	streptomycin	PROPN
elements-9604	91	51	.	.	PUNCT
elements-9604	92	1	transfection	transfection	NOUN
elements-9604	92	2	of	of	ADP
elements-9604	92	3	239	239	NUM
elements-9604	92	4	t	t	NOUN
elements-9604	92	5	cells	cell	NOUN
elements-9604	92	6	the	the	DET
elements-9604	92	7	pcep4	pcep4	NOUN
elements-9604	92	8	/	/	SYM
elements-9604	92	9	ll25x	ll25x	PROPN
elements-9604	92	10	dna	dna	PROPN
elements-9604	92	11	that	that	PRON
elements-9604	92	12	was	be	AUX
elements-9604	92	13	isolated	isolate	VERB
elements-9604	92	14	via	via	ADP
elements-9604	92	15	maxi	maxi	NOUN
elements-9604	92	16	-	-	PUNCT
elements-9604	92	17	prep	prep	NOUN
elements-9604	92	18	was	be	AUX
elements-9604	92	19	used	use	VERB
elements-9604	92	20	to	to	PART
elements-9604	92	21	transfect	transfect	VERB
elements-9604	92	22	293	293	NUM
elements-9604	92	23	t	t	NOUN
elements-9604	92	24	cells	cell	NOUN
elements-9604	92	25	by	by	ADP
elements-9604	92	26	following	follow	VERB
elements-9604	92	27	the	the	DET
elements-9604	92	28	lipofectamine	lipofectamine	ADJ
elements-9604	92	29	2000	2000	NUM
elements-9604	92	30	dna	dna	PROPN
elements-9604	92	31	transfection	transfection	NOUN
elements-9604	92	32	reagent	reagent	ADJ
elements-9604	92	33	protocol	protocol	NOUN
elements-9604	92	34	.	.	PUNCT
elements-9604	93	1	one	one	NUM
elements-9604	93	2	day	day	NOUN
elements-9604	93	3	before	before	ADP
elements-9604	93	4	transfection	transfection	NOUN
elements-9604	93	5	,	,	PUNCT
elements-9604	93	6	the	the	DET
elements-9604	93	7	293	293	NUM
elements-9604	93	8	t	t	NOUN
elements-9604	93	9	cells	cell	NOUN
elements-9604	93	10	were	be	AUX
elements-9604	93	11	trypsinized	trypsinize	VERB
elements-9604	93	12	and	and	CCONJ
elements-9604	93	13	resuspended	resuspend	VERB
elements-9604	93	14	into	into	ADP
elements-9604	93	15	a	a	DET
elements-9604	93	16	10	10	NUM
elements-9604	93	17	ml	ml	NOUN
elements-9604	93	18	/	/	SYM
elements-9604	93	19	t75	t75	NOUN
elements-9604	93	20	flask	flask	NOUN
elements-9604	93	21	.	.	PUNCT
elements-9604	94	1	using	use	VERB
elements-9604	94	2	a	a	DET
elements-9604	94	3	haemocytometer	haemocytometer	NOUN
elements-9604	94	4	,	,	PUNCT
elements-9604	94	5	the	the	DET
elements-9604	94	6	cell	cell	NOUN
elements-9604	94	7	number	number	NOUN
elements-9604	94	8	was	be	AUX
elements-9604	94	9	determined	determine	VERB
elements-9604	94	10	,	,	PUNCT
elements-9604	94	11	and	and	CCONJ
elements-9604	94	12	trypan	trypan	NOUN
elements-9604	94	13	blue	blue	NOUN
elements-9604	94	14	was	be	AUX
elements-9604	94	15	added	add	VERB
elements-9604	94	16	to	to	PART
elements-9604	94	17	determine	determine	VERB
elements-9604	94	18	cell	cell	NOUN
elements-9604	94	19	viability	viability	NOUN
elements-9604	94	20	.	.	PUNCT
elements-9604	95	1	the	the	DET
elements-9604	95	2	cells	cell	NOUN
elements-9604	95	3	were	be	AUX
elements-9604	95	4	seeded	seed	VERB
elements-9604	95	5	at	at	ADP
elements-9604	95	6	6×10	6×10	NUM
elements-9604	95	7	^	^	SYM
elements-9604	95	8	6	6	NUM
elements-9604	95	9	cells	cell	NOUN
elements-9604	95	10	/	/	SYM
elements-9604	95	11	t75	t75	NOUN
elements-9604	95	12	flask	flask	NOUN
elements-9604	95	13	.	.	PUNCT
elements-9604	96	1	on	on	ADP
elements-9604	96	2	the	the	DET
elements-9604	96	3	day	day	NOUN
elements-9604	96	4	of	of	ADP
elements-9604	96	5	transfection	transfection	NOUN
elements-9604	96	6	,	,	PUNCT
elements-9604	96	7	the	the	DET
elements-9604	96	8	culture	culture	NOUN
elements-9604	96	9	media	medium	NOUN
elements-9604	96	10	(	(	PUNCT
elements-9604	96	11	dmem	dmem	VERB
elements-9604	96	12	consisting	consist	VERB
elements-9604	96	13	of	of	ADP
elements-9604	96	14	10	10	NUM
elements-9604	96	15	%	%	NOUN
elements-9604	96	16	fbs	fb	NOUN
elements-9604	96	17	,	,	PUNCT
elements-9604	96	18	1x	1x	PROPN
elements-9604	96	19	pbs	pbs	PROPN
elements-9604	96	20	(	(	PUNCT
elements-9604	96	21	phosphate	phosphate	ADJ
elements-9604	96	22	buffer	buffer	NOUN
elements-9604	96	23	saline)/	saline)/	NOUN
elements-9604	96	24	strep	strep	NOUN
elements-9604	96	25	,	,	PUNCT
elements-9604	96	26	1x	1x	NUM
elements-9604	96	27	glutamine	glutamine	NOUN
elements-9604	96	28	)	)	PUNCT
elements-9604	96	29	replaced	replace	VERB
elements-9604	96	30	the	the	DET
elements-9604	96	31	old	old	ADJ
elements-9604	96	32	media	medium	NOUN
elements-9604	96	33	1	1	NUM
elements-9604	96	34	hour	hour	NOUN
elements-9604	96	35	before	before	ADP
elements-9604	96	36	transfection	transfection	NOUN
elements-9604	96	37	.	.	PUNCT
elements-9604	97	1	the	the	DET
elements-9604	97	2	transfection	transfection	NOUN
elements-9604	97	3	mixture	mixture	NOUN
elements-9604	97	4	was	be	AUX
elements-9604	97	5	prepared	prepare	VERB
elements-9604	97	6	using	use	VERB
elements-9604	97	7	500	500	NUM
elements-9604	97	8	µl	µl	NOUN
elements-9604	97	9	of	of	ADP
elements-9604	97	10	serum	serum	NOUN
elements-9604	97	11	-	-	PUNCT
elements-9604	97	12	free	free	ADJ
elements-9604	97	13	media	medium	NOUN
elements-9604	97	14	(	(	PUNCT
elements-9604	97	15	sfm	sfm	PROPN
elements-9604	97	16	ie	ie	X
elements-9604	97	17	.	.	PUNCT
elements-9604	97	18	opti	opti	PROPN
elements-9604	97	19	-	-	PUNCT
elements-9604	97	20	mem	mem	NOUN
elements-9604	97	21	)	)	PUNCT
elements-9604	97	22	+	+	CCONJ
elements-9604	97	23	10	10	NUM
elements-9604	97	24	µg	µg	ADP
elements-9604	97	25	of	of	ADP
elements-9604	97	26	maxi	maxi	ADJ
elements-9604	97	27	-	-	PUNCT
elements-9604	97	28	prep	prep	NOUN
elements-9604	97	29	dna	dna	NOUN
elements-9604	97	30	,	,	PUNCT
elements-9604	97	31	500	500	NUM
elements-9604	97	32	µl	µl	NOUN
elements-9604	97	33	of	of	ADP
elements-9604	97	34	sfm	sfm	PROPN
elements-9604	97	35	+	+	CCONJ
elements-9604	97	36	10	10	NUM
elements-9604	97	37	µl	µl	NOUN
elements-9604	97	38	of	of	ADP
elements-9604	97	39	lipofectamine	lipofectamine	ADJ
elements-9604	97	40	2000	2000	NUM
elements-9604	97	41	reagent	reagent	NOUN
elements-9604	97	42	,	,	PUNCT
elements-9604	97	43	and	and	CCONJ
elements-9604	97	44	dna	dna	PROPN
elements-9604	97	45	mix	mix	NOUN
elements-9604	97	46	,	,	PUNCT
elements-9604	97	47	which	which	PRON
elements-9604	97	48	was	be	AUX
elements-9604	97	49	incubated	incubate	VERB
elements-9604	97	50	before	before	ADP
elements-9604	97	51	at	at	ADP
elements-9604	97	52	room	room	NOUN
elements-9604	97	53	temperature	temperature	NOUN
elements-9604	97	54	for	for	ADP
elements-9604	97	55	20	20	NUM
elements-9604	97	56	minutes	minute	NOUN
elements-9604	97	57	.	.	PUNCT
elements-9604	98	1	the	the	DET
elements-9604	98	2	transfection	transfection	NOUN
elements-9604	98	3	mixture	mixture	NOUN
elements-9604	98	4	was	be	AUX
elements-9604	98	5	added	add	VERB
elements-9604	98	6	to	to	ADP
elements-9604	98	7	the	the	DET
elements-9604	98	8	cell	cell	NOUN
elements-9604	98	9	culture	culture	NOUN
elements-9604	98	10	and	and	CCONJ
elements-9604	98	11	incubated	incubate	VERB
elements-9604	98	12	overnight	overnight	ADV
elements-9604	98	13	at	at	ADP
elements-9604	98	14	37	37	NUM
elements-9604	98	15	°	°	NOUN
elements-9604	98	16	c	c	NOUN
elements-9604	98	17	,	,	PUNCT
elements-9604	98	18	5	5	NUM
elements-9604	98	19	%	%	NOUN
elements-9604	98	20	co2	co2	NOUN
elements-9604	98	21	,	,	PUNCT
elements-9604	98	22	and	and	CCONJ
elements-9604	98	23	70	70	NUM
elements-9604	98	24	%	%	NOUN
elements-9604	98	25	humidity	humidity	NOUN
elements-9604	98	26	.	.	PUNCT
elements-9604	99	1	cleavage	cleavage	NOUN
elements-9604	99	2	of	of	ADP
elements-9604	99	3	sivmac239gp140	sivmac239gp140	PROPN
elements-9604	99	4	into	into	ADP
elements-9604	99	5	monomeric	monomeric	ADJ
elements-9604	99	6	surface	surface	NOUN
elements-9604	99	7	subunit	subunit	NOUN
elements-9604	99	8	sivmac239gp120	sivmac239gp120	PROPN
elements-9604	99	9	and	and	CCONJ
elements-9604	99	10	ectodomain	ectodomain	VERB
elements-9604	99	11	sivmac239gp41	sivmac239gp41	PROPN
elements-9604	99	12	occurred	occur	VERB
elements-9604	99	13	once	once	SCONJ
elements-9604	99	14	the	the	DET
elements-9604	99	15	proteins	protein	NOUN
elements-9604	99	16	were	be	AUX
elements-9604	99	17	expressed	express	VERB
elements-9604	99	18	in	in	ADP
elements-9604	99	19	the	the	DET
elements-9604	99	20	293	293	NUM
elements-9604	99	21	t	t	NOUN
elements-9604	99	22	cells	cell	NOUN
elements-9604	99	23	.	.	PUNCT
elements-9604	100	1	one	one	NUM
elements-9604	100	2	day	day	NOUN
elements-9604	100	3	after	after	ADP
elements-9604	100	4	transfection	transfection	NOUN
elements-9604	100	5	,	,	PUNCT
elements-9604	100	6	the	the	DET
elements-9604	100	7	culture	culture	NOUN
elements-9604	100	8	media	medium	NOUN
elements-9604	100	9	was	be	AUX
elements-9604	100	10	replaced	replace	VERB
elements-9604	100	11	with	with	ADP
elements-9604	100	12	10	10	NUM
elements-9604	100	13	ml	ml	NOUN
elements-9604	100	14	of	of	ADP
elements-9604	100	15	sfm	sfm	NOUN
elements-9604	100	16	=	=	PRON
elements-9604	100	17	dmem	dmem	ADJ
elements-9604	100	18	(	(	PUNCT
elements-9604	100	19	1x	1x	NUM
elements-9604	100	20	pen	pen	PROPN
elements-9604	100	21	/	/	SYM
elements-9604	100	22	strep	strep	PROPN
elements-9604	100	23	,	,	PUNCT
elements-9604	100	24	1x	1x	NUM
elements-9604	100	25	glutamine	glutamine	NOUN
elements-9604	100	26	,	,	PUNCT
elements-9604	100	27	and	and	CCONJ
elements-9604	100	28	without	without	ADP
elements-9604	100	29	fbs	fb	NOUN
elements-9604	100	30	)	)	PUNCT
elements-9604	100	31	.	.	PUNCT
elements-9604	101	1	five	five	NUM
elements-9604	101	2	days	day	NOUN
elements-9604	101	3	after	after	ADP
elements-9604	101	4	transfection	transfection	NOUN
elements-9604	101	5	,	,	PUNCT
elements-9604	101	6	the	the	DET
elements-9604	101	7	culture	culture	NOUN
elements-9604	101	8	supernatant	supernatant	NOUN
elements-9604	101	9	was	be	AUX
elements-9604	101	10	transferred	transfer	VERB
elements-9604	101	11	into	into	ADP
elements-9604	101	12	15	15	NUM
elements-9604	101	13	ml	ml	ADP
elements-9604	101	14	conical	conical	ADJ
elements-9604	101	15	tubes	tube	NOUN
elements-9604	101	16	.	.	PUNCT
elements-9604	102	1	the	the	DET
elements-9604	102	2	tubes	tube	NOUN
elements-9604	102	3	were	be	AUX
elements-9604	102	4	centrifuged	centrifuge	VERB
elements-9604	102	5	at	at	ADP
elements-9604	102	6	3,000	3,000	NUM
elements-9604	102	7	rpm	rpm	NOUN
elements-9604	102	8	for	for	ADP
elements-9604	102	9	10	10	NUM
elements-9604	102	10	min	min	NOUN
elements-9604	102	11	to	to	PART
elements-9604	102	12	pellet	pellet	VERB
elements-9604	102	13	the	the	DET
elements-9604	102	14	debris	debris	NOUN
elements-9604	102	15	in	in	ADP
elements-9604	102	16	the	the	DET
elements-9604	102	17	tube	tube	NOUN
elements-9604	102	18	.	.	PUNCT
elements-9604	103	1	the	the	DET
elements-9604	103	2	supernatant	supernatant	NOUN
elements-9604	103	3	was	be	AUX
elements-9604	103	4	transferred	transfer	VERB
elements-9604	103	5	to	to	ADP
elements-9604	103	6	new	new	ADJ
elements-9604	103	7	15	15	NUM
elements-9604	103	8	ml	ml	NOUN
elements-9604	103	9	conical	conical	ADJ
elements-9604	103	10	tubes	tube	NOUN
elements-9604	103	11	.	.	PUNCT
elements-9604	104	1	protein	protein	NOUN
elements-9604	104	2	purification	purification	VERB
elements-9604	104	3	the	the	DET
elements-9604	104	4	purification	purification	NOUN
elements-9604	104	5	on	on	ADP
elements-9604	104	6	protein	protein	NOUN
elements-9604	104	7	a	a	DET
elements-9604	104	8	-	-	PUNCT
elements-9604	104	9	agarose	agarose	NOUN
elements-9604	104	10	protocol	protocol	NOUN
elements-9604	104	11	by	by	ADP
elements-9604	104	12	roche	roche	PROPN
elements-9604	104	13	was	be	AUX
elements-9604	104	14	followed	follow	VERB
elements-9604	104	15	to	to	PART
elements-9604	104	16	purify	purify	VERB
elements-9604	104	17	the	the	DET
elements-9604	104	18	protein	protein	NOUN
elements-9604	104	19	.	.	PUNCT
elements-9604	105	1	the	the	DET
elements-9604	105	2	resulting	result	VERB
elements-9604	105	3	eluate	eluate	ADJ
elements-9604	105	4	,	,	PUNCT
elements-9604	105	5	“	"	PUNCT
elements-9604	105	6	the	the	DET
elements-9604	105	7	isolation	isolation	NOUN
elements-9604	105	8	of	of	ADP
elements-9604	105	9	anti	anti	ADJ
elements-9604	105	10	-	-	ADJ
elements-9604	105	11	siv	siv	ADJ
elements-9604	105	12	monoclonal	monoclonal	NOUN
elements-9604	105	13	antibodies	antibody	NOUN
elements-9604	105	14	(	(	PUNCT
elements-9604	105	15	mab	mab	NOUN
elements-9604	105	16	)	)	PUNCT
elements-9604	105	17	will	will	AUX
elements-9604	105	18	allow	allow	VERB
elements-9604	105	19	a	a	DET
elements-9604	105	20	pre	pre	ADJ
elements-9604	105	21	-	-	ADJ
elements-9604	105	22	clinical	clinical	ADJ
elements-9604	105	23	evaluation	evaluation	NOUN
elements-9604	105	24	of	of	ADP
elements-9604	105	25	such	such	ADJ
elements-9604	105	26	antibodies	antibody	NOUN
elements-9604	105	27	in	in	ADP
elements-9604	105	28	the	the	DET
elements-9604	105	29	siv	siv	NOUN
elements-9604	105	30	-	-	PUNCT
elements-9604	105	31	rhesus	rhesus	NOUN
elements-9604	105	32	macaque	macaque	ADJ
elements-9604	105	33	model	model	NOUN
elements-9604	105	34	of	of	ADP
elements-9604	105	35	hiv	hiv	PROPN
elements-9604	105	36	/	/	SYM
elements-9604	105	37	aids	aids	PROPN
elements-9604	105	38	...	...	PUNCT
elements-9604	105	39	”	"	PUNCT
elements-9604	105	40	74	74	NUM
elements-9604	105	41	elements	element	NOUN
elements-9604	105	42	:	:	PUNCT
elements-9604	105	43	:	:	PUNCT
elements-9604	105	44	spring	spring	NOUN
elements-9604	105	45	2017	2017	NUM
elements-9604	105	46	containing	contain	VERB
elements-9604	105	47	the	the	DET
elements-9604	105	48	protein	protein	NOUN
elements-9604	105	49	,	,	PUNCT
elements-9604	105	50	was	be	AUX
elements-9604	105	51	mixed	mix	VERB
elements-9604	105	52	by	by	ADP
elements-9604	105	53	vortex	vortex	NOUN
elements-9604	105	54	and	and	CCONJ
elements-9604	105	55	transferred	transfer	VERB
elements-9604	105	56	into	into	ADP
elements-9604	105	57	a	a	DET
elements-9604	105	58	dialysis	dialysis	NOUN
elements-9604	105	59	cassette	cassette	NOUN
elements-9604	105	60	(	(	PUNCT
elements-9604	105	61	10,000	10,000	NUM
elements-9604	105	62	molecular	molecular	ADJ
elements-9604	105	63	weight	weight	NOUN
elements-9604	105	64	cut	cut	NOUN
elements-9604	105	65	-	-	PUNCT
elements-9604	105	66	off	off	ADP
elements-9604	105	67	mwco	mwco	NOUN
elements-9604	105	68	)	)	PUNCT
elements-9604	105	69	.	.	PUNCT
elements-9604	106	1	dialysis	dialysis	NOUN
elements-9604	106	2	was	be	AUX
elements-9604	106	3	performed	perform	VERB
elements-9604	106	4	overnight	overnight	ADV
elements-9604	106	5	in	in	ADP
elements-9604	106	6	1xpbs	1xpbs	NUM
elements-9604	106	7	at	at	ADP
elements-9604	106	8	4	4	NUM
elements-9604	106	9	°	°	ADP
elements-9604	106	10	c	c	NOUN
elements-9604	106	11	and	and	CCONJ
elements-9604	106	12	was	be	AUX
elements-9604	106	13	slowly	slowly	ADV
elements-9604	106	14	stirred	stir	VERB
elements-9604	106	15	.	.	PUNCT
elements-9604	107	1	the	the	DET
elements-9604	107	2	next	next	ADJ
elements-9604	107	3	day	day	NOUN
elements-9604	107	4	,	,	PUNCT
elements-9604	107	5	a	a	DET
elements-9604	107	6	centrifugal	centrifugal	ADJ
elements-9604	107	7	filter	filter	NOUN
elements-9604	107	8	unit	unit	NOUN
elements-9604	107	9	(	(	PUNCT
elements-9604	107	10	amicon	amicon	PROPN
elements-9604	107	11	ultracel	ultracel	PROPN
elements-9604	107	12	10,000	10,000	NUM
elements-9604	107	13	muco	muco	NOUN
elements-9604	107	14	)	)	PUNCT
elements-9604	107	15	was	be	AUX
elements-9604	107	16	used	use	VERB
elements-9604	107	17	to	to	PART
elements-9604	107	18	concentrate	concentrate	VERB
elements-9604	107	19	the	the	DET
elements-9604	107	20	protein	protein	NOUN
elements-9604	107	21	eluate	eluate	ADJ
elements-9604	107	22	until	until	SCONJ
elements-9604	107	23	the	the	DET
elements-9604	107	24	remaining	remain	VERB
elements-9604	107	25	buffer	buffer	NOUN
elements-9604	107	26	was	be	AUX
elements-9604	107	27	around	around	ADV
elements-9604	107	28	100	100	NUM
elements-9604	107	29	-	-	SYM
elements-9604	107	30	200	200	NUM
elements-9604	107	31	µl	µl	NOUN
elements-9604	107	32	.	.	PUNCT
elements-9604	108	1	the	the	DET
elements-9604	108	2	unit	unit	NOUN
elements-9604	108	3	was	be	AUX
elements-9604	108	4	centrifuged	centrifuge	VERB
elements-9604	108	5	at	at	ADP
elements-9604	108	6	3,000	3,000	NUM
elements-9604	108	7	rpm	rpm	NOUN
elements-9604	108	8	for	for	ADP
elements-9604	108	9	30	30	NUM
elements-9604	108	10	minutes	minute	NOUN
elements-9604	108	11	.	.	PUNCT
elements-9604	109	1	protein	protein	NOUN
elements-9604	109	2	/	/	SYM
elements-9604	109	3	antibody	antibody	NOUN
elements-9604	109	4	characterization	characterization	NOUN
elements-9604	109	5	by	by	ADP
elements-9604	109	6	elisa	elisa	PROPN
elements-9604	109	7	(	(	PUNCT
elements-9604	109	8	enzymelinked	enzymelinke	VERB
elements-9604	109	9	immuno	immuno	ADJ
elements-9604	109	10	-	-	PUNCT
elements-9604	109	11	sorbant	sorbant	ADJ
elements-9604	109	12	assay	assay	NOUN
elements-9604	109	13	)	)	PUNCT
elements-9604	109	14	antigens	antigen	NOUN
elements-9604	109	15	(	(	PUNCT
elements-9604	109	16	sivmac239gp140	sivmac239gp140	PROPN
elements-9604	109	17	,	,	PUNCT
elements-9604	109	18	sivmac239gp120	sivmac239gp120	NOUN
elements-9604	109	19	,	,	PUNCT
elements-9604	109	20	and	and	CCONJ
elements-9604	109	21	control	control	PROPN
elements-9604	109	22	bsa	bsa	PROPN
elements-9604	109	23	)	)	PUNCT
elements-9604	109	24	were	be	AUX
elements-9604	109	25	prepared	prepare	VERB
elements-9604	109	26	at	at	ADP
elements-9604	109	27	1	1	NUM
elements-9604	109	28	ng/µl	ng/µl	NUM
elements-9604	109	29	with	with	ADP
elements-9604	109	30	1xpbs	1xpbs	NUM
elements-9604	109	31	.	.	PUNCT
elements-9604	110	1	a	a	DET
elements-9604	110	2	plate	plate	NOUN
elements-9604	110	3	was	be	AUX
elements-9604	110	4	divided	divide	VERB
elements-9604	110	5	so	so	SCONJ
elements-9604	110	6	that	that	SCONJ
elements-9604	110	7	100	100	NUM
elements-9604	110	8	µl	µl	NOUN
elements-9604	110	9	of	of	ADP
elements-9604	110	10	each	each	DET
elements-9604	110	11	diluted	dilute	VERB
elements-9604	110	12	antigen	antigen	NOUN
elements-9604	110	13	was	be	AUX
elements-9604	110	14	added	add	VERB
elements-9604	110	15	to	to	ADP
elements-9604	110	16	each	each	DET
elements-9604	110	17	corresponding	correspond	VERB
elements-9604	110	18	well	well	ADV
elements-9604	110	19	.	.	PUNCT
elements-9604	111	1	the	the	DET
elements-9604	111	2	coated	coated	ADJ
elements-9604	111	3	elisa	elisa	NOUN
elements-9604	111	4	plate	plate	NOUN
elements-9604	111	5	was	be	AUX
elements-9604	111	6	incubated	incubate	VERB
elements-9604	111	7	for	for	ADP
elements-9604	111	8	1	1	NUM
elements-9604	111	9	hour	hour	NOUN
elements-9604	111	10	at	at	ADP
elements-9604	111	11	37	37	NUM
elements-9604	111	12	°	°	NOUN
elements-9604	111	13	c	c	NOUN
elements-9604	111	14	.	.	PUNCT
elements-9604	112	1	after	after	ADP
elements-9604	112	2	incubation	incubation	NOUN
elements-9604	112	3	,	,	PUNCT
elements-9604	112	4	the	the	DET
elements-9604	112	5	plate	plate	NOUN
elements-9604	112	6	was	be	AUX
elements-9604	112	7	washed	wash	VERB
elements-9604	112	8	once	once	ADV
elements-9604	112	9	with	with	ADP
elements-9604	112	10	1xpbs	1xpbs	NUM
elements-9604	112	11	containing	contain	VERB
elements-9604	112	12	0.5	0.5	NUM
elements-9604	112	13	%	%	NOUN
elements-9604	112	14	tween20	tween20	NOUN
elements-9604	112	15	.	.	PUNCT
elements-9604	113	1	the	the	DET
elements-9604	113	2	tray	tray	NOUN
elements-9604	113	3	was	be	AUX
elements-9604	113	4	blocked	block	VERB
elements-9604	113	5	by	by	ADP
elements-9604	113	6	adding	add	VERB
elements-9604	113	7	500	500	NUM
elements-9604	113	8	µl	µl	NOUN
elements-9604	113	9	of	of	ADP
elements-9604	113	10	5	5	NUM
elements-9604	113	11	%	%	NOUN
elements-9604	113	12	skim	skim	NOUN
elements-9604	113	13	milk	milk	NOUN
elements-9604	113	14	in	in	ADP
elements-9604	113	15	1xpbs	1xpbs	NUM
elements-9604	113	16	.	.	PUNCT
elements-9604	114	1	the	the	DET
elements-9604	114	2	tray	tray	NOUN
elements-9604	114	3	was	be	AUX
elements-9604	114	4	sealed	seal	VERB
elements-9604	114	5	and	and	CCONJ
elements-9604	114	6	incubated	incubate	VERB
elements-9604	114	7	for	for	ADP
elements-9604	114	8	1	1	NUM
elements-9604	114	9	hour	hour	NOUN
elements-9604	114	10	at	at	ADP
elements-9604	114	11	37	37	NUM
elements-9604	114	12	°	°	NOUN
elements-9604	114	13	c	c	NOUN
elements-9604	114	14	.	.	PUNCT
elements-9604	115	1	a	a	DET
elements-9604	115	2	1000x	1000x	NUM
elements-9604	115	3	serial	serial	ADJ
elements-9604	115	4	dilution	dilution	NOUN
elements-9604	115	5	of	of	ADP
elements-9604	115	6	the	the	DET
elements-9604	115	7	primary	primary	ADJ
elements-9604	115	8	antibody	antibody	NOUN
elements-9604	115	9	/	/	SYM
elements-9604	115	10	protein	protein	NOUN
elements-9604	115	11	was	be	AUX
elements-9604	115	12	prepared	prepare	VERB
elements-9604	115	13	,	,	PUNCT
elements-9604	115	14	beginning	begin	VERB
elements-9604	115	15	with	with	ADP
elements-9604	115	16	10	10	NUM
elements-9604	115	17	µg/µl	µg/µl	NUM
elements-9604	115	18	.	.	PUNCT
elements-9604	116	1	the	the	DET
elements-9604	116	2	dilutions	dilution	NOUN
elements-9604	116	3	were	be	AUX
elements-9604	116	4	10	10	NUM
elements-9604	116	5	µg/µl	µg/µl	NUM
elements-9604	116	6	,	,	PUNCT
elements-9604	116	7	1	1	NUM
elements-9604	116	8	µg/µl	µg/µl	NUM
elements-9604	116	9	,	,	PUNCT
elements-9604	116	10	and	and	CCONJ
elements-9604	116	11	0.1	0.1	NUM
elements-9604	116	12	µg/µl	µg/µl	NUM
elements-9604	116	13	.	.	PUNCT
elements-9604	117	1	the	the	DET
elements-9604	117	2	dilutions	dilution	NOUN
elements-9604	117	3	were	be	AUX
elements-9604	117	4	prepared	prepare	VERB
elements-9604	117	5	using	use	VERB
elements-9604	117	6	1xpbs	1xpbs	NUM
elements-9604	117	7	/	/	SYM
elements-9604	117	8	fbs	fbs	ADJ
elements-9604	117	9	2	2	NUM
elements-9604	117	10	%	%	NOUN
elements-9604	117	11	,	,	PUNCT
elements-9604	117	12	and	and	CCONJ
elements-9604	117	13	50	50	NUM
elements-9604	117	14	µl	µl	NOUN
elements-9604	117	15	of	of	ADP
elements-9604	117	16	the	the	DET
elements-9604	117	17	antibody/	antibody/	NUM
elements-9604	117	18	protein	protein	NOUN
elements-9604	117	19	dilution	dilution	NOUN
elements-9604	117	20	was	be	AUX
elements-9604	117	21	added	add	VERB
elements-9604	117	22	to	to	ADP
elements-9604	117	23	each	each	DET
elements-9604	117	24	well	well	ADV
elements-9604	117	25	.	.	PUNCT
elements-9604	118	1	for	for	ADP
elements-9604	118	2	each	each	DET
elements-9604	118	3	antigen	antigen	NOUN
elements-9604	118	4	,	,	PUNCT
elements-9604	118	5	one	one	NUM
elements-9604	118	6	of	of	ADP
elements-9604	118	7	each	each	DET
elements-9604	118	8	dilution	dilution	NOUN
elements-9604	118	9	was	be	AUX
elements-9604	118	10	added	add	VERB
elements-9604	118	11	.	.	PUNCT
elements-9604	119	1	therefore	therefore	ADV
elements-9604	119	2	,	,	PUNCT
elements-9604	119	3	each	each	DET
elements-9604	119	4	antigen	antigen	NOUN
elements-9604	119	5	would	would	AUX
elements-9604	119	6	have	have	VERB
elements-9604	119	7	three	three	NUM
elements-9604	119	8	different	different	ADJ
elements-9604	119	9	dilutions	dilution	NOUN
elements-9604	119	10	.	.	PUNCT
elements-9604	120	1	the	the	DET
elements-9604	120	2	tray	tray	NOUN
elements-9604	120	3	was	be	AUX
elements-9604	120	4	incubated	incubate	VERB
elements-9604	120	5	for	for	ADP
elements-9604	120	6	1	1	NUM
elements-9604	120	7	hour	hour	NOUN
elements-9604	120	8	at	at	ADP
elements-9604	120	9	37	37	NUM
elements-9604	120	10	°	°	ADP
elements-9604	120	11	c	c	NOUN
elements-9604	120	12	.	.	PUNCT
elements-9604	121	1	the	the	DET
elements-9604	121	2	plate	plate	NOUN
elements-9604	121	3	was	be	AUX
elements-9604	121	4	washed	wash	VERB
elements-9604	121	5	10	10	NUM
elements-9604	121	6	times	time	NOUN
elements-9604	121	7	with	with	ADP
elements-9604	121	8	1xpbs	1xpbs	NUM
elements-9604	121	9	containing	contain	VERB
elements-9604	121	10	0.5	0.5	NUM
elements-9604	121	11	%	%	NOUN
elements-9604	121	12	tween20	tween20	NOUN
elements-9604	121	13	and	and	CCONJ
elements-9604	121	14	flipped	flip	VERB
elements-9604	121	15	over	over	ADP
elements-9604	121	16	on	on	ADP
elements-9604	121	17	a	a	DET
elements-9604	121	18	paper	paper	NOUN
elements-9604	121	19	towel	towel	NOUN
elements-9604	121	20	to	to	PART
elements-9604	121	21	dry	dry	VERB
elements-9604	121	22	for	for	ADP
elements-9604	121	23	1	1	NUM
elements-9604	121	24	minute	minute	NOUN
elements-9604	121	25	.	.	PUNCT
elements-9604	122	1	after	after	ADP
elements-9604	122	2	drying	dry	VERB
elements-9604	122	3	the	the	DET
elements-9604	122	4	tray	tray	NOUN
elements-9604	122	5	,	,	PUNCT
elements-9604	122	6	50	50	NUM
elements-9604	122	7	µl	µl	NOUN
elements-9604	122	8	of	of	ADP
elements-9604	122	9	secondary	secondary	ADJ
elements-9604	122	10	antibodies	antibody	NOUN
elements-9604	122	11	,	,	PUNCT
elements-9604	122	12	hrp	hrp	NOUN
elements-9604	122	13	-	-	PUNCT
elements-9604	122	14	conjugate	conjugate	ADJ
elements-9604	122	15	anti	anti	ADJ
elements-9604	122	16	-	-	ADJ
elements-9604	122	17	human	human	ADJ
elements-9604	122	18	igg	igg	PROPN
elements-9604	122	19	antibodies	antibodie	VERB
elements-9604	122	20	(	(	PUNCT
elements-9604	122	21	1000x	1000x	NUM
elements-9604	122	22	dilution	dilution	NOUN
elements-9604	122	23	in	in	ADP
elements-9604	122	24	5	5	NUM
elements-9604	122	25	%	%	NOUN
elements-9604	122	26	skim	skim	NOUN
elements-9604	122	27	milk	milk	NOUN
elements-9604	122	28	)	)	PUNCT
elements-9604	122	29	,	,	PUNCT
elements-9604	122	30	were	be	AUX
elements-9604	122	31	added	add	VERB
elements-9604	122	32	to	to	ADP
elements-9604	122	33	all	all	PRON
elements-9604	122	34	of	of	ADP
elements-9604	122	35	the	the	DET
elements-9604	122	36	wells	well	NOUN
elements-9604	122	37	.	.	PUNCT
elements-9604	123	1	the	the	DET
elements-9604	123	2	tray	tray	NOUN
elements-9604	123	3	was	be	AUX
elements-9604	123	4	sealed	seal	VERB
elements-9604	123	5	and	and	CCONJ
elements-9604	123	6	incubated	incubate	VERB
elements-9604	123	7	for	for	ADP
elements-9604	123	8	1	1	NUM
elements-9604	123	9	hour	hour	NOUN
elements-9604	123	10	at	at	ADP
elements-9604	123	11	37	37	NUM
elements-9604	123	12	°	°	NOUN
elements-9604	123	13	c	c	NOUN
elements-9604	123	14	.	.	PUNCT
elements-9604	124	1	the	the	DET
elements-9604	124	2	plate	plate	NOUN
elements-9604	124	3	was	be	AUX
elements-9604	124	4	washed	wash	VERB
elements-9604	124	5	5	5	NUM
elements-9604	124	6	times	time	NOUN
elements-9604	124	7	with	with	ADP
elements-9604	124	8	1xpbs	1xpbs	NUM
elements-9604	124	9	containing	contain	VERB
elements-9604	124	10	0.5	0.5	NUM
elements-9604	124	11	%	%	NOUN
elements-9604	124	12	tween20	tween20	NOUN
elements-9604	124	13	and	and	CCONJ
elements-9604	124	14	flipped	flip	VERB
elements-9604	124	15	over	over	ADP
elements-9604	124	16	to	to	PART
elements-9604	124	17	dry	dry	VERB
elements-9604	124	18	on	on	ADP
elements-9604	124	19	a	a	DET
elements-9604	124	20	paper	paper	NOUN
elements-9604	124	21	towel	towel	NOUN
elements-9604	124	22	for	for	ADP
elements-9604	124	23	1	1	NUM
elements-9604	124	24	minute	minute	NOUN
elements-9604	124	25	,	,	PUNCT
elements-9604	124	26	and	and	CCONJ
elements-9604	124	27	100	100	NUM
elements-9604	124	28	µl	µl	NOUN
elements-9604	124	29	of	of	ADP
elements-9604	124	30	tmb	tmb	NOUN
elements-9604	124	31	solution	solution	NOUN
elements-9604	124	32	was	be	AUX
elements-9604	124	33	added	add	VERB
elements-9604	124	34	.	.	PUNCT
elements-9604	125	1	the	the	DET
elements-9604	125	2	plate	plate	NOUN
elements-9604	125	3	was	be	AUX
elements-9604	125	4	incubated	incubate	VERB
elements-9604	125	5	for	for	ADP
elements-9604	125	6	30	30	NUM
elements-9604	125	7	minutes	minute	NOUN
elements-9604	125	8	at	at	ADP
elements-9604	125	9	room	room	NOUN
elements-9604	125	10	temperature	temperature	NOUN
elements-9604	125	11	.	.	PUNCT
elements-9604	126	1	once	once	SCONJ
elements-9604	126	2	blue	blue	ADJ
elements-9604	126	3	color	color	NOUN
elements-9604	126	4	was	be	AUX
elements-9604	126	5	observed	observe	VERB
elements-9604	126	6	,	,	PUNCT
elements-9604	126	7	the	the	DET
elements-9604	126	8	reaction	reaction	NOUN
elements-9604	126	9	was	be	AUX
elements-9604	126	10	stopped	stop	VERB
elements-9604	126	11	with	with	ADP
elements-9604	126	12	100	100	NUM
elements-9604	126	13	µl	µl	NOUN
elements-9604	126	14	of	of	ADP
elements-9604	126	15	tmb	tmb	PROPN
elements-9604	126	16	stop	stop	NOUN
elements-9604	126	17	solution	solution	NOUN
elements-9604	126	18	.	.	PUNCT
elements-9604	127	1	the	the	DET
elements-9604	127	2	absorbances	absorbance	NOUN
elements-9604	127	3	of	of	ADP
elements-9604	127	4	the	the	DET
elements-9604	127	5	wells	well	NOUN
elements-9604	127	6	were	be	AUX
elements-9604	127	7	read	read	VERB
elements-9604	127	8	on	on	ADP
elements-9604	127	9	wallac	wallac	PROPN
elements-9604	127	10	(	(	PUNCT
elements-9604	127	11	victor	victor	NOUN
elements-9604	127	12	plate	plate	NOUN
elements-9604	127	13	reader	reader	NOUN
elements-9604	127	14	)	)	PUNCT
elements-9604	127	15	at	at	ADP
elements-9604	127	16	450	450	NUM
elements-9604	127	17	nm	nm	NOUN
elements-9604	127	18	.	.	PUNCT
elements-9604	128	1	protein	protein	NOUN
elements-9604	128	2	charcterization	charcterization	NOUN
elements-9604	128	3	by	by	ADP
elements-9604	128	4	coomassie	coomassie	ADJ
elements-9604	128	5	blue	blue	ADJ
elements-9604	128	6	staining	stain	VERB
elements-9604	128	7	a	a	DET
elements-9604	128	8	12	12	NUM
elements-9604	128	9	%	%	NOUN
elements-9604	128	10	agarose	agarose	NOUN
elements-9604	128	11	gel	gel	NOUN
elements-9604	128	12	was	be	AUX
elements-9604	128	13	prepared	prepare	VERB
elements-9604	128	14	.	.	PUNCT
elements-9604	129	1	samples	sample	NOUN
elements-9604	129	2	to	to	PART
elements-9604	129	3	be	be	AUX
elements-9604	129	4	loaded	load	VERB
elements-9604	129	5	into	into	ADP
elements-9604	129	6	the	the	DET
elements-9604	129	7	wells	well	NOUN
elements-9604	129	8	consisted	consist	VERB
elements-9604	129	9	of	of	ADP
elements-9604	129	10	5	5	NUM
elements-9604	129	11	µg	µg	NOUN
elements-9604	129	12	of	of	ADP
elements-9604	129	13	each	each	DET
elements-9604	129	14	envelope	envelope	NOUN
elements-9604	129	15	protein	protein	NOUN
elements-9604	129	16	(	(	PUNCT
elements-9604	129	17	gp120	gp120	PROPN
elements-9604	129	18	and	and	CCONJ
elements-9604	129	19	gp140	gp140	PROPN
elements-9604	129	20	)	)	PUNCT
elements-9604	129	21	and	and	CCONJ
elements-9604	129	22	1xpbs	1xpbs	NUM
elements-9604	129	23	was	be	AUX
elements-9604	129	24	added	add	VERB
elements-9604	129	25	to	to	ADP
elements-9604	129	26	each	each	DET
elements-9604	129	27	sample	sample	NOUN
elements-9604	129	28	until	until	SCONJ
elements-9604	129	29	a	a	DET
elements-9604	129	30	total	total	ADJ
elements-9604	129	31	volume	volume	NOUN
elements-9604	129	32	of	of	ADP
elements-9604	129	33	10	10	NUM
elements-9604	129	34	µl	µl	NOUN
elements-9604	129	35	was	be	AUX
elements-9604	129	36	achieved	achieve	VERB
elements-9604	129	37	.	.	PUNCT
elements-9604	130	1	to	to	ADP
elements-9604	130	2	the	the	DET
elements-9604	130	3	10	10	NUM
elements-9604	130	4	µl	µl	NOUN
elements-9604	130	5	of	of	ADP
elements-9604	130	6	each	each	DET
elements-9604	130	7	sample	sample	NOUN
elements-9604	130	8	,	,	PUNCT
elements-9604	130	9	10	10	NUM
elements-9604	130	10	µl	µl	NOUN
elements-9604	130	11	of	of	ADP
elements-9604	130	12	2x	2x	NUM
elements-9604	130	13	sample	sample	NOUN
elements-9604	130	14	loading	loading	NOUN
elements-9604	130	15	buffer	buffer	NOUN
elements-9604	130	16	was	be	AUX
elements-9604	130	17	added	add	VERB
elements-9604	130	18	for	for	ADP
elements-9604	130	19	a	a	DET
elements-9604	130	20	total	total	ADJ
elements-9604	130	21	volume	volume	NOUN
elements-9604	130	22	of	of	ADP
elements-9604	130	23	20	20	NUM
elements-9604	130	24	µl	µl	NOUN
elements-9604	130	25	.	.	PUNCT
elements-9604	131	1	the	the	DET
elements-9604	131	2	samples	sample	NOUN
elements-9604	131	3	were	be	AUX
elements-9604	131	4	boiled	boil	VERB
elements-9604	131	5	at	at	ADP
elements-9604	131	6	100	100	NUM
elements-9604	131	7	°	°	ADP
elements-9604	131	8	c	c	NOUN
elements-9604	131	9	for	for	ADP
elements-9604	131	10	5	5	NUM
elements-9604	131	11	minutes	minute	NOUN
elements-9604	131	12	,	,	PUNCT
elements-9604	131	13	spun	spin	VERB
elements-9604	131	14	down	down	ADP
elements-9604	131	15	,	,	PUNCT
elements-9604	131	16	and	and	CCONJ
elements-9604	131	17	loaded	load	VERB
elements-9604	131	18	onto	onto	ADP
elements-9604	131	19	the	the	DET
elements-9604	131	20	gel	gel	NOUN
elements-9604	131	21	.	.	PUNCT
elements-9604	132	1	lane	lane	PROPN
elements-9604	132	2	1	1	NUM
elements-9604	132	3	contained	contain	VERB
elements-9604	132	4	the	the	DET
elements-9604	132	5	ladder	ladder	NOUN
elements-9604	132	6	,	,	PUNCT
elements-9604	132	7	lane	lane	NOUN
elements-9604	132	8	2	2	NUM
elements-9604	132	9	contained	contain	VERB
elements-9604	132	10	the	the	DET
elements-9604	132	11	env	env	NOUN
elements-9604	132	12	glycoprotein	glycoprotein	NOUN
elements-9604	132	13	,	,	PUNCT
elements-9604	132	14	gp120	gp120	NOUN
elements-9604	132	15	,	,	PUNCT
elements-9604	132	16	and	and	CCONJ
elements-9604	132	17	lane	lane	NOUN
elements-9604	132	18	3	3	NUM
elements-9604	132	19	contained	contain	VERB
elements-9604	132	20	the	the	DET
elements-9604	132	21	env	env	NOUN
elements-9604	132	22	glycoprotein	glycoprotein	NOUN
elements-9604	132	23	,	,	PUNCT
elements-9604	132	24	gp140	gp140	PROPN
elements-9604	132	25	.	.	PUNCT
elements-9604	133	1	after	after	SCONJ
elements-9604	133	2	the	the	DET
elements-9604	133	3	gel	gel	NOUN
elements-9604	133	4	was	be	AUX
elements-9604	133	5	run	run	VERB
elements-9604	133	6	,	,	PUNCT
elements-9604	133	7	the	the	DET
elements-9604	133	8	gel	gel	NOUN
elements-9604	133	9	was	be	AUX
elements-9604	133	10	stained	stain	VERB
elements-9604	133	11	with	with	ADP
elements-9604	133	12	coomassie	coomassie	ADJ
elements-9604	133	13	blue	blue	NOUN
elements-9604	133	14	,	,	PUNCT
elements-9604	133	15	and	and	CCONJ
elements-9604	133	16	the	the	DET
elements-9604	133	17	protein	protein	NOUN
elements-9604	133	18	bands	band	NOUN
elements-9604	133	19	were	be	AUX
elements-9604	133	20	observed	observe	VERB
elements-9604	133	21	.	.	PUNCT
elements-9604	134	1	protein	protein	NOUN
elements-9604	134	2	/	/	SYM
elements-9604	134	3	antibody	antibody	NOUN
elements-9604	134	4	characterization	characterization	NOUN
elements-9604	134	5	by	by	ADP
elements-9604	134	6	western	western	ADJ
elements-9604	134	7	blotting	blotting	NOUN
elements-9604	134	8	a	a	DET
elements-9604	134	9	12	12	NUM
elements-9604	134	10	%	%	NOUN
elements-9604	134	11	agarose	agarose	NOUN
elements-9604	134	12	gel	gel	NOUN
elements-9604	134	13	was	be	AUX
elements-9604	134	14	prepared	prepare	VERB
elements-9604	134	15	.	.	PUNCT
elements-9604	135	1	samples	sample	NOUN
elements-9604	135	2	to	to	PART
elements-9604	135	3	be	be	AUX
elements-9604	135	4	loaded	load	VERB
elements-9604	135	5	into	into	ADP
elements-9604	135	6	the	the	DET
elements-9604	135	7	wells	well	NOUN
elements-9604	135	8	consisted	consist	VERB
elements-9604	135	9	of	of	ADP
elements-9604	135	10	5	5	NUM
elements-9604	135	11	µg	µg	NOUN
elements-9604	135	12	of	of	ADP
elements-9604	135	13	each	each	DET
elements-9604	135	14	protein	protein	NOUN
elements-9604	135	15	/	/	SYM
elements-9604	135	16	antibody	antibody	NOUN
elements-9604	135	17	(	(	PUNCT
elements-9604	135	18	su24	su24	PROPN
elements-9604	135	19	,	,	PUNCT
elements-9604	135	20	su343	su343	PROPN
elements-9604	135	21	,	,	PUNCT
elements-9604	135	22	and	and	CCONJ
elements-9604	135	23	ll25x	ll25x	NOUN
elements-9604	135	24	)	)	PUNCT
elements-9604	135	25	and	and	CCONJ
elements-9604	135	26	1xpbs	1xpbs	NUM
elements-9604	135	27	was	be	AUX
elements-9604	135	28	added	add	VERB
elements-9604	135	29	to	to	ADP
elements-9604	135	30	each	each	DET
elements-9604	135	31	sample	sample	NOUN
elements-9604	135	32	until	until	SCONJ
elements-9604	135	33	a	a	DET
elements-9604	135	34	total	total	ADJ
elements-9604	135	35	volume	volume	NOUN
elements-9604	135	36	of	of	ADP
elements-9604	135	37	10	10	NUM
elements-9604	135	38	µl	µl	NOUN
elements-9604	135	39	was	be	AUX
elements-9604	135	40	achieved	achieve	VERB
elements-9604	135	41	.	.	PUNCT
elements-9604	136	1	to	to	ADP
elements-9604	136	2	the	the	DET
elements-9604	136	3	10	10	NUM
elements-9604	136	4	µl	µl	NOUN
elements-9604	136	5	of	of	ADP
elements-9604	136	6	each	each	DET
elements-9604	136	7	sample	sample	NOUN
elements-9604	136	8	,	,	PUNCT
elements-9604	136	9	10	10	NUM
elements-9604	136	10	µl	µl	NOUN
elements-9604	136	11	of	of	ADP
elements-9604	136	12	2x	2x	NUM
elements-9604	136	13	sample	sample	NOUN
elements-9604	136	14	loading	loading	NOUN
elements-9604	136	15	buffer	buffer	NOUN
elements-9604	136	16	was	be	AUX
elements-9604	136	17	added	add	VERB
elements-9604	136	18	for	for	ADP
elements-9604	136	19	a	a	DET
elements-9604	136	20	total	total	ADJ
elements-9604	136	21	volume	volume	NOUN
elements-9604	136	22	of	of	ADP
elements-9604	136	23	20	20	NUM
elements-9604	136	24	µl	µl	NOUN
elements-9604	136	25	.	.	PUNCT
elements-9604	137	1	the	the	DET
elements-9604	137	2	samples	sample	NOUN
elements-9604	137	3	were	be	AUX
elements-9604	137	4	boiled	boil	VERB
elements-9604	137	5	at	at	ADP
elements-9604	137	6	100	100	NUM
elements-9604	137	7	°	°	ADP
elements-9604	137	8	c	c	NOUN
elements-9604	137	9	for	for	ADP
elements-9604	137	10	5	5	NUM
elements-9604	137	11	minutes	minute	NOUN
elements-9604	137	12	,	,	PUNCT
elements-9604	137	13	spun	spin	VERB
elements-9604	137	14	down	down	ADP
elements-9604	137	15	,	,	PUNCT
elements-9604	137	16	and	and	CCONJ
elements-9604	137	17	loaded	load	VERB
elements-9604	137	18	onto	onto	ADP
elements-9604	137	19	the	the	DET
elements-9604	137	20	gel	gel	NOUN
elements-9604	137	21	.	.	PUNCT
elements-9604	138	1	lane	lane	PROPN
elements-9604	138	2	1	1	NUM
elements-9604	138	3	contained	contain	VERB
elements-9604	138	4	the	the	DET
elements-9604	138	5	ladder	ladder	NOUN
elements-9604	138	6	,	,	PUNCT
elements-9604	138	7	lane	lane	NOUN
elements-9604	138	8	2	2	NUM
elements-9604	138	9	contained	contain	VERB
elements-9604	138	10	su24	su24	PROPN
elements-9604	138	11	-	-	PUNCT
elements-9604	138	12	fc	fc	PROPN
elements-9604	138	13	,	,	PUNCT
elements-9604	138	14	lane	lane	NOUN
elements-9604	138	15	3	3	NUM
elements-9604	138	16	contained	contain	VERB
elements-9604	138	17	su343	su343	PROPN
elements-9604	138	18	-	-	PUNCT
elements-9604	138	19	fc	fc	PROPN
elements-9604	138	20	,	,	PUNCT
elements-9604	138	21	and	and	CCONJ
elements-9604	138	22	lane	lane	NOUN
elements-9604	138	23	4	4	NUM
elements-9604	138	24	contained	contain	VERB
elements-9604	138	25	ll25xfc	ll25xfc	PROPN
elements-9604	138	26	.	.	PUNCT
elements-9604	139	1	after	after	SCONJ
elements-9604	139	2	the	the	DET
elements-9604	139	3	gel	gel	NOUN
elements-9604	139	4	was	be	AUX
elements-9604	139	5	run	run	VERB
elements-9604	139	6	,	,	PUNCT
elements-9604	139	7	the	the	DET
elements-9604	139	8	proteins	protein	NOUN
elements-9604	139	9	in	in	ADP
elements-9604	139	10	the	the	DET
elements-9604	139	11	gel	gel	NOUN
elements-9604	139	12	were	be	AUX
elements-9604	139	13	transferred	transfer	VERB
elements-9604	139	14	to	to	ADP
elements-9604	139	15	a	a	DET
elements-9604	139	16	nitrocellulose	nitrocellulose	NOUN
elements-9604	139	17	membrane	membrane	NOUN
elements-9604	139	18	.	.	PUNCT
elements-9604	140	1	the	the	DET
elements-9604	140	2	membrane	membrane	NOUN
elements-9604	140	3	was	be	AUX
elements-9604	140	4	blocked	block	VERB
elements-9604	140	5	by	by	ADP
elements-9604	140	6	using	use	VERB
elements-9604	140	7	5	5	NUM
elements-9604	140	8	%	%	NOUN
elements-9604	140	9	skim	skim	NOUN
elements-9604	140	10	milk	milk	NOUN
elements-9604	140	11	to	to	PART
elements-9604	140	12	prevent	prevent	VERB
elements-9604	140	13	nonspecific	nonspecific	ADJ
elements-9604	140	14	binding	binding	NOUN
elements-9604	140	15	and	and	CCONJ
elements-9604	140	16	was	be	AUX
elements-9604	140	17	treated	treat	VERB
elements-9604	140	18	with	with	ADP
elements-9604	140	19	the	the	DET
elements-9604	140	20	primary	primary	ADJ
elements-9604	140	21	antibody	antibody	NOUN
elements-9604	140	22	,	,	PUNCT
elements-9604	140	23	antiigg	antiigg	NOUN
elements-9604	140	24	-	-	PUNCT
elements-9604	140	25	hrp	hrp	PROPN
elements-9604	140	26	.	.	PUNCT
elements-9604	141	1	the	the	DET
elements-9604	141	2	membrane	membrane	NOUN
elements-9604	141	3	was	be	AUX
elements-9604	141	4	left	leave	VERB
elements-9604	141	5	for	for	ADP
elements-9604	141	6	1	1	NUM
elements-9604	141	7	hour	hour	NOUN
elements-9604	141	8	in	in	ADP
elements-9604	141	9	5	5	NUM
elements-9604	141	10	%	%	NOUN
elements-9604	141	11	skim	skim	NOUN
elements-9604	141	12	milk	milk	NOUN
elements-9604	141	13	that	that	PRON
elements-9604	141	14	was	be	AUX
elements-9604	141	15	prepared	prepare	VERB
elements-9604	141	16	with	with	ADP
elements-9604	141	17	1xpbs	1xpbs	NUM
elements-9604	141	18	.	.	PUNCT
elements-9604	142	1	the	the	DET
elements-9604	142	2	gel	gel	NOUN
elements-9604	142	3	was	be	AUX
elements-9604	142	4	washed	wash	VERB
elements-9604	142	5	3	3	NUM
elements-9604	142	6	times	time	NOUN
elements-9604	142	7	with	with	ADP
elements-9604	142	8	1xpbs	1xpbs	NUM
elements-9604	142	9	for	for	ADP
elements-9604	142	10	10	10	NUM
elements-9604	142	11	minutes	minute	NOUN
elements-9604	142	12	each	each	PRON
elements-9604	142	13	.	.	PUNCT
elements-9604	143	1	this	this	DET
elements-9604	143	2	membrane	membrane	NOUN
elements-9604	143	3	,	,	PUNCT
elements-9604	143	4	blotting	blot	VERB
elements-9604	143	5	gel	gel	NOUN
elements-9604	143	6	1	1	NUM
elements-9604	143	7	,	,	PUNCT
elements-9604	143	8	consisted	consist	VERB
elements-9604	143	9	of	of	ADP
elements-9604	143	10	su24	su24	PROPN
elements-9604	143	11	,	,	PUNCT
elements-9604	143	12	su343	su343	PROPN
elements-9604	143	13	,	,	PUNCT
elements-9604	143	14	and	and	CCONJ
elements-9604	143	15	ll25x	ll25x	NOUN
elements-9604	143	16	and	and	CCONJ
elements-9604	143	17	used	use	VERB
elements-9604	143	18	anti	anti	ADJ
elements-9604	143	19	-	-	ADJ
elements-9604	143	20	human	human	ADJ
elements-9604	143	21	igg	igg	PROPN
elements-9604	143	22	-	-	PUNCT
elements-9604	143	23	hrp	hrp	PROPN
elements-9604	143	24	as	as	ADP
elements-9604	143	25	the	the	DET
elements-9604	143	26	primary	primary	ADJ
elements-9604	143	27	antibody	antibody	NOUN
elements-9604	143	28	.	.	PUNCT
elements-9604	144	1	three	three	NUM
elements-9604	144	2	12	12	NUM
elements-9604	144	3	%	%	NOUN
elements-9604	144	4	agarose	agarose	NOUN
elements-9604	144	5	gels	gel	NOUN
elements-9604	144	6	were	be	AUX
elements-9604	144	7	prepared	prepare	VERB
elements-9604	144	8	.	.	PUNCT
elements-9604	145	1	samples	sample	NOUN
elements-9604	145	2	to	to	PART
elements-9604	145	3	be	be	AUX
elements-9604	145	4	loaded	load	VERB
elements-9604	145	5	into	into	ADP
elements-9604	145	6	the	the	DET
elements-9604	145	7	wells	well	NOUN
elements-9604	145	8	consisted	consist	VERB
elements-9604	145	9	of	of	ADP
elements-9604	145	10	5	5	NUM
elements-9604	145	11	µg	µg	NOUN
elements-9604	145	12	of	of	ADP
elements-9604	145	13	each	each	DET
elements-9604	145	14	protein/	protein/	NUM
elements-9604	145	15	antibody	antibody	NOUN
elements-9604	145	16	(	(	PUNCT
elements-9604	145	17	sivmac239gp120	sivmac239gp120	ADJ
elements-9604	145	18	and	and	CCONJ
elements-9604	145	19	sivmac239gp140	sivmac239gp140	VERB
elements-9604	145	20	)	)	PUNCT
elements-9604	145	21	and	and	CCONJ
elements-9604	145	22	10	10	NUM
elements-9604	145	23	µl	µl	NOUN
elements-9604	145	24	of	of	ADP
elements-9604	145	25	2x	2x	NUM
elements-9604	145	26	sample	sample	NOUN
elements-9604	145	27	loading	loading	NOUN
elements-9604	145	28	buffer	buffer	NOUN
elements-9604	145	29	,	,	PUNCT
elements-9604	145	30	and	and	CCONJ
elements-9604	145	31	1xpbs	1xpbs	NUM
elements-9604	145	32	was	be	AUX
elements-9604	145	33	added	add	VERB
elements-9604	145	34	to	to	ADP
elements-9604	145	35	each	each	DET
elements-9604	145	36	sample	sample	NOUN
elements-9604	145	37	until	until	SCONJ
elements-9604	145	38	a	a	DET
elements-9604	145	39	total	total	ADJ
elements-9604	145	40	volume	volume	NOUN
elements-9604	145	41	of	of	ADP
elements-9604	145	42	10	10	NUM
elements-9604	145	43	µl	µl	NOUN
elements-9604	145	44	was	be	AUX
elements-9604	145	45	achieved	achieve	VERB
elements-9604	145	46	.	.	PUNCT
elements-9604	146	1	the	the	DET
elements-9604	146	2	samples	sample	NOUN
elements-9604	146	3	were	be	AUX
elements-9604	146	4	boiled	boil	VERB
elements-9604	146	5	at	at	ADP
elements-9604	146	6	100	100	NUM
elements-9604	146	7	°	°	ADP
elements-9604	146	8	c	c	NOUN
elements-9604	146	9	for	for	ADP
elements-9604	146	10	5	5	NUM
elements-9604	146	11	minutes	minute	NOUN
elements-9604	146	12	,	,	PUNCT
elements-9604	146	13	spun	spin	VERB
elements-9604	146	14	down	down	ADP
elements-9604	146	15	,	,	PUNCT
elements-9604	146	16	and	and	CCONJ
elements-9604	146	17	loaded	load	VERB
elements-9604	146	18	onto	onto	ADP
elements-9604	146	19	the	the	DET
elements-9604	146	20	gel	gel	NOUN
elements-9604	146	21	.	.	PUNCT
elements-9604	147	1	lane	lane	PROPN
elements-9604	147	2	1	1	NUM
elements-9604	147	3	contained	contain	VERB
elements-9604	147	4	the	the	DET
elements-9604	147	5	ladder	ladder	NOUN
elements-9604	147	6	,	,	PUNCT
elements-9604	147	7	lane	lane	NOUN
elements-9604	147	8	2	2	NUM
elements-9604	147	9	contained	contain	VERB
elements-9604	147	10	the	the	DET
elements-9604	147	11	env	env	NOUN
elements-9604	147	12	glycoprotein	glycoprotein	NOUN
elements-9604	147	13	,	,	PUNCT
elements-9604	147	14	gp120	gp120	NOUN
elements-9604	147	15	,	,	PUNCT
elements-9604	147	16	and	and	CCONJ
elements-9604	147	17	lane	lane	NOUN
elements-9604	147	18	3	3	NUM
elements-9604	147	19	contained	contain	VERB
elements-9604	147	20	the	the	DET
elements-9604	147	21	env	env	NOUN
elements-9604	147	22	glycoprotein	glycoprotein	NOUN
elements-9604	147	23	,	,	PUNCT
elements-9604	147	24	gp140	gp140	PROPN
elements-9604	147	25	.	.	PUNCT
elements-9604	148	1	after	after	SCONJ
elements-9604	148	2	the	the	DET
elements-9604	148	3	gel	gel	NOUN
elements-9604	148	4	was	be	AUX
elements-9604	148	5	run	run	VERB
elements-9604	148	6	,	,	PUNCT
elements-9604	148	7	the	the	DET
elements-9604	148	8	proteins	protein	NOUN
elements-9604	148	9	in	in	ADP
elements-9604	148	10	the	the	DET
elements-9604	148	11	gel	gel	NOUN
elements-9604	148	12	were	be	AUX
elements-9604	148	13	transferred	transfer	VERB
elements-9604	148	14	to	to	ADP
elements-9604	148	15	a	a	DET
elements-9604	148	16	nitrocellulose	nitrocellulose	NOUN
elements-9604	148	17	membrane	membrane	NOUN
elements-9604	148	18	.	.	PUNCT
elements-9604	149	1	for	for	ADP
elements-9604	149	2	the	the	DET
elements-9604	149	3	first	first	ADJ
elements-9604	149	4	gel	gel	NOUN
elements-9604	149	5	,	,	PUNCT
elements-9604	149	6	blotting	blot	VERB
elements-9604	149	7	gel	gel	NOUN
elements-9604	149	8	2	2	NUM
elements-9604	149	9	,	,	PUNCT
elements-9604	149	10	the	the	DET
elements-9604	149	11	membrane	membrane	NOUN
elements-9604	149	12	was	be	AUX
elements-9604	149	13	blocked	block	VERB
elements-9604	149	14	by	by	ADP
elements-9604	149	15	using	use	VERB
elements-9604	149	16	5	5	NUM
elements-9604	149	17	%	%	NOUN
elements-9604	149	18	skim	skim	NOUN
elements-9604	149	19	milk	milk	NOUN
elements-9604	149	20	to	to	PART
elements-9604	149	21	prevent	prevent	VERB
elements-9604	149	22	nonspecific	nonspecific	ADJ
elements-9604	149	23	binding	binding	NOUN
elements-9604	149	24	and	and	CCONJ
elements-9604	149	25	was	be	AUX
elements-9604	149	26	treated	treat	VERB
elements-9604	149	27	with	with	ADP
elements-9604	149	28	the	the	DET
elements-9604	149	29	primary	primary	ADJ
elements-9604	149	30	antibody	antibody	NOUN
elements-9604	149	31	,	,	PUNCT
elements-9604	149	32	su24	su24	PROPN
elements-9604	149	33	.	.	PUNCT
elements-9604	150	1	the	the	DET
elements-9604	150	2	membrane	membrane	NOUN
elements-9604	150	3	was	be	AUX
elements-9604	150	4	left	leave	VERB
elements-9604	150	5	for	for	ADP
elements-9604	150	6	1	1	NUM
elements-9604	150	7	hour	hour	NOUN
elements-9604	150	8	in	in	ADP
elements-9604	150	9	5	5	NUM
elements-9604	150	10	%	%	NOUN
elements-9604	150	11	skim	skim	NOUN
elements-9604	150	12	milk	milk	NOUN
elements-9604	150	13	that	that	PRON
elements-9604	150	14	was	be	AUX
elements-9604	150	15	prepared	prepare	VERB
elements-9604	150	16	with	with	ADP
elements-9604	150	17	1xpbs	1xpbs	NUM
elements-9604	150	18	.	.	PUNCT
elements-9604	151	1	the	the	DET
elements-9604	151	2	gel	gel	NOUN
elements-9604	151	3	was	be	AUX
elements-9604	151	4	washed	wash	VERB
elements-9604	151	5	3	3	NUM
elements-9604	151	6	times	time	NOUN
elements-9604	151	7	with	with	ADP
elements-9604	151	8	1xpbs	1xpbs	NUM
elements-9604	151	9	for	for	ADP
elements-9604	151	10	10	10	NUM
elements-9604	151	11	minutes	minute	NOUN
elements-9604	151	12	each	each	PRON
elements-9604	151	13	.	.	PUNCT
elements-9604	152	1	anti	anti	ADJ
elements-9604	152	2	-	-	ADJ
elements-9604	152	3	human	human	ADJ
elements-9604	152	4	igg	igg	PROPN
elements-9604	152	5	-	-	PUNCT
elements-9604	152	6	hrp	hrp	PROPN
elements-9604	152	7	was	be	AUX
elements-9604	152	8	added	add	VERB
elements-9604	152	9	as	as	ADP
elements-9604	152	10	the	the	DET
elements-9604	152	11	secondary	secondary	ADJ
elements-9604	152	12	antibody	antibody	NOUN
elements-9604	152	13	.	.	PUNCT
elements-9604	153	1	the	the	DET
elements-9604	153	2	gel	gel	NOUN
elements-9604	153	3	was	be	AUX
elements-9604	153	4	washed	wash	VERB
elements-9604	153	5	3	3	NUM
elements-9604	153	6	times	time	NOUN
elements-9604	153	7	with	with	ADP
elements-9604	153	8	1xpbs	1xpbs	NUM
elements-9604	153	9	for	for	ADP
elements-9604	153	10	10	10	NUM
elements-9604	153	11	minutes	minute	NOUN
elements-9604	153	12	each	each	PRON
elements-9604	153	13	.	.	PUNCT
elements-9604	154	1	the	the	DET
elements-9604	154	2	chemiluminescence	chemiluminescence	NOUN
elements-9604	154	3	reagent	reagent	NOUN
elements-9604	154	4	was	be	AUX
elements-9604	154	5	added	add	VERB
elements-9604	154	6	to	to	ADP
elements-9604	154	7	the	the	DET
elements-9604	154	8	gel	gel	NOUN
elements-9604	154	9	.	.	PUNCT
elements-9604	155	1	the	the	DET
elements-9604	155	2	gel	gel	NOUN
elements-9604	155	3	was	be	AUX
elements-9604	155	4	observed	observe	VERB
elements-9604	155	5	under	under	ADP
elements-9604	155	6	uv	uv	NOUN
elements-9604	155	7	light	light	NOUN
elements-9604	155	8	,	,	PUNCT
elements-9604	155	9	and	and	CCONJ
elements-9604	155	10	pictures	picture	NOUN
elements-9604	155	11	were	be	AUX
elements-9604	155	12	taken	take	VERB
elements-9604	155	13	75	75	NUM
elements-9604	155	14	the	the	DET
elements-9604	155	15	road	road	NOUN
elements-9604	155	16	to	to	ADP
elements-9604	155	17	a	a	DET
elements-9604	155	18	cure	cure	NOUN
elements-9604	155	19	using	use	VERB
elements-9604	155	20	the	the	DET
elements-9604	155	21	chemiluminescence	chemiluminescence	NOUN
elements-9604	155	22	dock	dock	NOUN
elements-9604	155	23	.	.	PUNCT
elements-9604	156	1	the	the	DET
elements-9604	156	2	first	first	ADJ
elements-9604	156	3	gel	gel	NOUN
elements-9604	156	4	contained	contain	VERB
elements-9604	156	5	sivmac239gp120	sivmac239gp120	PROPN
elements-9604	156	6	and	and	CCONJ
elements-9604	156	7	sivmac239gp140	sivmac239gp140	PROPN
elements-9604	156	8	,	,	PUNCT
elements-9604	156	9	used	use	VERB
elements-9604	156	10	su24	su24	PROPN
elements-9604	156	11	as	as	ADP
elements-9604	156	12	the	the	DET
elements-9604	156	13	primary	primary	ADJ
elements-9604	156	14	antibody	antibody	NOUN
elements-9604	156	15	,	,	PUNCT
elements-9604	156	16	and	and	CCONJ
elements-9604	156	17	used	use	VERB
elements-9604	156	18	anti	anti	ADJ
elements-9604	156	19	-	-	ADJ
elements-9604	156	20	human	human	ADJ
elements-9604	156	21	igghrp	igghrp	NOUN
elements-9604	156	22	as	as	ADP
elements-9604	156	23	the	the	DET
elements-9604	156	24	secondary	secondary	ADJ
elements-9604	156	25	antibody	antibody	NOUN
elements-9604	156	26	.	.	PUNCT
elements-9604	157	1	for	for	ADP
elements-9604	157	2	the	the	DET
elements-9604	157	3	second	second	ADJ
elements-9604	157	4	gel	gel	NOUN
elements-9604	157	5	,	,	PUNCT
elements-9604	157	6	blotting	blot	VERB
elements-9604	157	7	gel	gel	NOUN
elements-9604	157	8	2	2	NUM
elements-9604	157	9	,	,	PUNCT
elements-9604	157	10	the	the	DET
elements-9604	157	11	membrane	membrane	NOUN
elements-9604	157	12	was	be	AUX
elements-9604	157	13	blocked	block	VERB
elements-9604	157	14	by	by	ADP
elements-9604	157	15	using	use	VERB
elements-9604	157	16	5	5	NUM
elements-9604	157	17	%	%	NOUN
elements-9604	157	18	skim	skim	NOUN
elements-9604	157	19	milk	milk	NOUN
elements-9604	157	20	to	to	PART
elements-9604	157	21	prevent	prevent	VERB
elements-9604	157	22	nonspecific	nonspecific	ADJ
elements-9604	157	23	binding	binding	NOUN
elements-9604	157	24	and	and	CCONJ
elements-9604	157	25	was	be	AUX
elements-9604	157	26	treated	treat	VERB
elements-9604	157	27	with	with	ADP
elements-9604	157	28	the	the	DET
elements-9604	157	29	primary	primary	ADJ
elements-9604	157	30	antibody	antibody	NOUN
elements-9604	157	31	,	,	PUNCT
elements-9604	157	32	su343	su343	PROPN
elements-9604	157	33	.	.	PUNCT
elements-9604	158	1	the	the	DET
elements-9604	158	2	membrane	membrane	NOUN
elements-9604	158	3	was	be	AUX
elements-9604	158	4	left	leave	VERB
elements-9604	158	5	for	for	ADP
elements-9604	158	6	1	1	NUM
elements-9604	158	7	hour	hour	NOUN
elements-9604	158	8	in	in	ADP
elements-9604	158	9	5	5	NUM
elements-9604	158	10	%	%	NOUN
elements-9604	158	11	skim	skim	NOUN
elements-9604	158	12	milk	milk	NOUN
elements-9604	158	13	that	that	PRON
elements-9604	158	14	was	be	AUX
elements-9604	158	15	prepared	prepare	VERB
elements-9604	158	16	with	with	ADP
elements-9604	158	17	1xpbs	1xpbs	NUM
elements-9604	158	18	.	.	PUNCT
elements-9604	159	1	the	the	DET
elements-9604	159	2	gel	gel	NOUN
elements-9604	159	3	was	be	AUX
elements-9604	159	4	washed	wash	VERB
elements-9604	159	5	3	3	NUM
elements-9604	159	6	times	time	NOUN
elements-9604	159	7	with	with	ADP
elements-9604	159	8	1xpbs	1xpbs	NUM
elements-9604	159	9	for	for	ADP
elements-9604	159	10	10	10	NUM
elements-9604	159	11	minutes	minute	NOUN
elements-9604	159	12	each	each	PRON
elements-9604	159	13	.	.	PUNCT
elements-9604	160	1	anti	anti	ADJ
elements-9604	160	2	-	-	ADJ
elements-9604	160	3	human	human	ADJ
elements-9604	160	4	igg	igg	PROPN
elements-9604	160	5	-	-	PUNCT
elements-9604	160	6	hrp	hrp	PROPN
elements-9604	160	7	was	be	AUX
elements-9604	160	8	added	add	VERB
elements-9604	160	9	as	as	ADP
elements-9604	160	10	the	the	DET
elements-9604	160	11	secondary	secondary	ADJ
elements-9604	160	12	antibody	antibody	NOUN
elements-9604	160	13	.	.	PUNCT
elements-9604	161	1	the	the	DET
elements-9604	161	2	gel	gel	NOUN
elements-9604	161	3	was	be	AUX
elements-9604	161	4	washed	wash	VERB
elements-9604	161	5	3	3	NUM
elements-9604	161	6	times	time	NOUN
elements-9604	161	7	with	with	ADP
elements-9604	161	8	1xpbs	1xpbs	NUM
elements-9604	161	9	for	for	ADP
elements-9604	161	10	10	10	NUM
elements-9604	161	11	minutes	minute	NOUN
elements-9604	161	12	each	each	PRON
elements-9604	161	13	.	.	PUNCT
elements-9604	162	1	the	the	DET
elements-9604	162	2	chemiluminescence	chemiluminescence	NOUN
elements-9604	162	3	reagent	reagent	NOUN
elements-9604	162	4	was	be	AUX
elements-9604	162	5	added	add	VERB
elements-9604	162	6	to	to	ADP
elements-9604	162	7	the	the	DET
elements-9604	162	8	gel	gel	NOUN
elements-9604	162	9	.	.	PUNCT
elements-9604	163	1	the	the	DET
elements-9604	163	2	gel	gel	NOUN
elements-9604	163	3	was	be	AUX
elements-9604	163	4	observed	observe	VERB
elements-9604	163	5	under	under	ADP
elements-9604	163	6	uv	uv	NOUN
elements-9604	163	7	light	light	NOUN
elements-9604	163	8	,	,	PUNCT
elements-9604	163	9	and	and	CCONJ
elements-9604	163	10	pictures	picture	NOUN
elements-9604	163	11	were	be	AUX
elements-9604	163	12	taken	take	VERB
elements-9604	163	13	using	use	VERB
elements-9604	163	14	the	the	DET
elements-9604	163	15	chemiluminescence	chemiluminescence	NOUN
elements-9604	163	16	dock	dock	NOUN
elements-9604	163	17	.	.	PUNCT
elements-9604	164	1	the	the	DET
elements-9604	164	2	second	second	ADJ
elements-9604	164	3	gel	gel	NOUN
elements-9604	164	4	contained	contain	VERB
elements-9604	164	5	sivmac239gp120	sivmac239gp120	PROPN
elements-9604	164	6	and	and	CCONJ
elements-9604	164	7	sivmac239gp140	sivmac239gp140	PROPN
elements-9604	164	8	,	,	PUNCT
elements-9604	164	9	used	use	VERB
elements-9604	164	10	su343	su343	PROPN
elements-9604	164	11	as	as	ADP
elements-9604	164	12	the	the	DET
elements-9604	164	13	primary	primary	ADJ
elements-9604	164	14	antibody	antibody	NOUN
elements-9604	164	15	,	,	PUNCT
elements-9604	164	16	and	and	CCONJ
elements-9604	164	17	used	use	VERB
elements-9604	164	18	anti	anti	ADJ
elements-9604	164	19	-	-	ADJ
elements-9604	164	20	human	human	ADJ
elements-9604	164	21	igg	igg	PROPN
elements-9604	164	22	-	-	PUNCT
elements-9604	164	23	hrp	hrp	PROPN
elements-9604	164	24	as	as	ADP
elements-9604	164	25	the	the	DET
elements-9604	164	26	secondary	secondary	ADJ
elements-9604	164	27	antibody	antibody	NOUN
elements-9604	164	28	.	.	PUNCT
elements-9604	165	1	for	for	ADP
elements-9604	165	2	the	the	DET
elements-9604	165	3	third	third	ADJ
elements-9604	165	4	gel	gel	NOUN
elements-9604	165	5	,	,	PUNCT
elements-9604	165	6	blotting	blot	VERB
elements-9604	165	7	gel	gel	NOUN
elements-9604	165	8	2	2	NUM
elements-9604	165	9	,	,	PUNCT
elements-9604	165	10	the	the	DET
elements-9604	165	11	membrane	membrane	NOUN
elements-9604	165	12	was	be	AUX
elements-9604	165	13	blocked	block	VERB
elements-9604	165	14	by	by	ADP
elements-9604	165	15	using	use	VERB
elements-9604	165	16	5	5	NUM
elements-9604	165	17	%	%	NOUN
elements-9604	165	18	skim	skim	NOUN
elements-9604	165	19	milk	milk	NOUN
elements-9604	165	20	to	to	PART
elements-9604	165	21	prevent	prevent	VERB
elements-9604	165	22	nonspecific	nonspecific	ADJ
elements-9604	165	23	binding	binding	NOUN
elements-9604	165	24	and	and	CCONJ
elements-9604	165	25	was	be	AUX
elements-9604	165	26	treated	treat	VERB
elements-9604	165	27	with	with	ADP
elements-9604	165	28	the	the	DET
elements-9604	165	29	primary	primary	ADJ
elements-9604	165	30	antibody	antibody	NOUN
elements-9604	165	31	,	,	PUNCT
elements-9604	165	32	ll25x	ll25x	PROPN
elements-9604	165	33	.	.	PUNCT
elements-9604	166	1	the	the	DET
elements-9604	166	2	membrane	membrane	NOUN
elements-9604	166	3	was	be	AUX
elements-9604	166	4	left	leave	VERB
elements-9604	166	5	for	for	ADP
elements-9604	166	6	1	1	NUM
elements-9604	166	7	hour	hour	NOUN
elements-9604	166	8	in	in	ADP
elements-9604	166	9	5	5	NUM
elements-9604	166	10	%	%	NOUN
elements-9604	166	11	skim	skim	NOUN
elements-9604	166	12	milk	milk	NOUN
elements-9604	166	13	that	that	PRON
elements-9604	166	14	was	be	AUX
elements-9604	166	15	prepared	prepare	VERB
elements-9604	166	16	with	with	ADP
elements-9604	166	17	1xpbs	1xpbs	NUM
elements-9604	166	18	.	.	PUNCT
elements-9604	167	1	the	the	DET
elements-9604	167	2	gel	gel	NOUN
elements-9604	167	3	was	be	AUX
elements-9604	167	4	washed	wash	VERB
elements-9604	167	5	3	3	NUM
elements-9604	167	6	times	time	NOUN
elements-9604	167	7	with	with	ADP
elements-9604	167	8	1xpbs	1xpbs	NUM
elements-9604	167	9	for	for	ADP
elements-9604	167	10	10	10	NUM
elements-9604	167	11	minutes	minute	NOUN
elements-9604	167	12	each	each	PRON
elements-9604	167	13	.	.	PUNCT
elements-9604	168	1	anti	anti	ADJ
elements-9604	168	2	-	-	ADJ
elements-9604	168	3	human	human	ADJ
elements-9604	168	4	igg	igg	PROPN
elements-9604	168	5	-	-	PUNCT
elements-9604	168	6	hrp	hrp	PROPN
elements-9604	168	7	was	be	AUX
elements-9604	168	8	added	add	VERB
elements-9604	168	9	as	as	ADP
elements-9604	168	10	the	the	DET
elements-9604	168	11	secondary	secondary	ADJ
elements-9604	168	12	antibody	antibody	NOUN
elements-9604	168	13	.	.	PUNCT
elements-9604	169	1	the	the	DET
elements-9604	169	2	gel	gel	NOUN
elements-9604	169	3	was	be	AUX
elements-9604	169	4	washed	wash	VERB
elements-9604	169	5	3	3	NUM
elements-9604	169	6	times	time	NOUN
elements-9604	169	7	with	with	ADP
elements-9604	169	8	1xpbs	1xpbs	NUM
elements-9604	169	9	for	for	ADP
elements-9604	169	10	10	10	NUM
elements-9604	169	11	minutes	minute	NOUN
elements-9604	169	12	each	each	PRON
elements-9604	169	13	.	.	PUNCT
elements-9604	170	1	the	the	DET
elements-9604	170	2	chemiluminescence	chemiluminescence	NOUN
elements-9604	170	3	reagent	reagent	NOUN
elements-9604	170	4	was	be	AUX
elements-9604	170	5	added	add	VERB
elements-9604	170	6	to	to	ADP
elements-9604	170	7	the	the	DET
elements-9604	170	8	gel	gel	NOUN
elements-9604	170	9	.	.	PUNCT
elements-9604	171	1	the	the	DET
elements-9604	171	2	gel	gel	NOUN
elements-9604	171	3	was	be	AUX
elements-9604	171	4	observed	observe	VERB
elements-9604	171	5	under	under	ADP
elements-9604	171	6	uv	uv	NOUN
elements-9604	171	7	light	light	NOUN
elements-9604	171	8	,	,	PUNCT
elements-9604	171	9	and	and	CCONJ
elements-9604	171	10	pictures	picture	NOUN
elements-9604	171	11	were	be	AUX
elements-9604	171	12	taken	take	VERB
elements-9604	171	13	using	use	VERB
elements-9604	171	14	the	the	DET
elements-9604	171	15	chemiluminescence	chemiluminescence	NOUN
elements-9604	171	16	dock	dock	NOUN
elements-9604	171	17	.	.	PUNCT
elements-9604	172	1	the	the	DET
elements-9604	172	2	third	third	ADJ
elements-9604	172	3	gel	gel	NOUN
elements-9604	172	4	contained	contain	VERB
elements-9604	172	5	sivmac239gp120	sivmac239gp120	PROPN
elements-9604	172	6	and	and	CCONJ
elements-9604	172	7	sivmac239gp140	sivmac239gp140	PROPN
elements-9604	172	8	,	,	PUNCT
elements-9604	172	9	used	use	VERB
elements-9604	172	10	ll25x	ll25x	NOUN
elements-9604	172	11	as	as	ADP
elements-9604	172	12	the	the	DET
elements-9604	172	13	primary	primary	ADJ
elements-9604	172	14	antibody	antibody	NOUN
elements-9604	172	15	,	,	PUNCT
elements-9604	172	16	and	and	CCONJ
elements-9604	172	17	used	use	VERB
elements-9604	172	18	anti	anti	ADJ
elements-9604	172	19	-	-	ADJ
elements-9604	172	20	human	human	ADJ
elements-9604	172	21	igg	igg	PROPN
elements-9604	172	22	-	-	PUNCT
elements-9604	172	23	hrp	hrp	PROPN
elements-9604	172	24	as	as	ADP
elements-9604	172	25	the	the	DET
elements-9604	172	26	secondary	secondary	ADJ
elements-9604	172	27	antibody	antibody	NOUN
elements-9604	172	28	.	.	PUNCT
elements-9604	173	1	results	result	NOUN
elements-9604	173	2	after	after	ADP
elements-9604	173	3	pcr	pcr	NOUN
elements-9604	173	4	,	,	PUNCT
elements-9604	173	5	the	the	DET
elements-9604	173	6	bright	bright	ADJ
elements-9604	173	7	band	band	NOUN
elements-9604	173	8	was	be	AUX
elements-9604	173	9	observed	observe	VERB
elements-9604	173	10	,	,	PUNCT
elements-9604	173	11	as	as	SCONJ
elements-9604	173	12	shown	show	VERB
elements-9604	173	13	in	in	ADP
elements-9604	173	14	figure	figure	NOUN
elements-9604	173	15	1a	1a	NOUN
elements-9604	173	16	.	.	PUNCT
elements-9604	174	1	the	the	DET
elements-9604	174	2	location	location	NOUN
elements-9604	174	3	of	of	ADP
elements-9604	174	4	the	the	DET
elements-9604	174	5	band	band	NOUN
elements-9604	174	6	is	be	AUX
elements-9604	174	7	approximately	approximately	ADV
elements-9604	174	8	at	at	ADP
elements-9604	174	9	1092	1092	NUM
elements-9604	174	10	bp	bp	PROPN
elements-9604	174	11	,	,	PUNCT
elements-9604	174	12	which	which	PRON
elements-9604	174	13	is	be	AUX
elements-9604	174	14	the	the	DET
elements-9604	174	15	size	size	NOUN
elements-9604	174	16	of	of	ADP
elements-9604	174	17	the	the	DET
elements-9604	174	18	ll25x	ll25x	PROPN
elements-9604	174	19	fragment	fragment	NOUN
elements-9604	174	20	.	.	PUNCT
elements-9604	175	1	this	this	PRON
elements-9604	175	2	indicates	indicate	VERB
elements-9604	175	3	that	that	SCONJ
elements-9604	175	4	the	the	DET
elements-9604	175	5	ll25x	ll25x	PROPN
elements-9604	175	6	fragment	fragment	NOUN
elements-9604	175	7	was	be	AUX
elements-9604	175	8	successfully	successfully	ADV
elements-9604	175	9	amplified	amplify	VERB
elements-9604	175	10	.	.	PUNCT
elements-9604	176	1	the	the	DET
elements-9604	176	2	pcr	pcr	PROPN
elements-9604	176	3	products	product	NOUN
elements-9604	176	4	,	,	PUNCT
elements-9604	176	5	corresponding	correspond	VERB
elements-9604	176	6	to	to	ADP
elements-9604	176	7	a	a	DET
elements-9604	176	8	codon	codon	NOUN
elements-9604	176	9	-	-	PUNCT
elements-9604	176	10	optimized	optimize	VERB
elements-9604	176	11	version	version	NOUN
elements-9604	176	12	of	of	ADP
elements-9604	176	13	ll25x	ll25x	NOUN
elements-9604	176	14	plus	plus	CCONJ
elements-9604	176	15	the	the	DET
elements-9604	176	16	hinge	hinge	NOUN
elements-9604	176	17	region	region	NOUN
elements-9604	176	18	(	(	PUNCT
elements-9604	176	19	h	h	NOUN
elements-9604	176	20	)	)	PUNCT
elements-9604	176	21	of	of	ADP
elements-9604	176	22	a	a	DET
elements-9604	176	23	human	human	ADJ
elements-9604	176	24	igg1	igg1	NOUN
elements-9604	176	25	,	,	PUNCT
elements-9604	176	26	were	be	AUX
elements-9604	176	27	extracted	extract	VERB
elements-9604	176	28	from	from	ADP
elements-9604	176	29	the	the	DET
elements-9604	176	30	gel	gel	NOUN
elements-9604	176	31	.	.	PUNCT
elements-9604	177	1	the	the	DET
elements-9604	177	2	amplified	amplify	VERB
elements-9604	177	3	ll25x	ll25x	NOUN
elements-9604	177	4	fragments	fragment	NOUN
elements-9604	177	5	were	be	AUX
elements-9604	177	6	double	double	ADV
elements-9604	177	7	digested	digest	VERB
elements-9604	177	8	with	with	ADP
elements-9604	177	9	kpni	kpni	NOUN
elements-9604	177	10	and	and	CCONJ
elements-9604	177	11	sapi	sapi	NOUN
elements-9604	177	12	,	,	PUNCT
elements-9604	177	13	and	and	CCONJ
elements-9604	177	14	the	the	DET
elements-9604	177	15	double	double	ADJ
elements-9604	177	16	digest	digest	NOUN
elements-9604	177	17	products	product	NOUN
elements-9604	177	18	were	be	AUX
elements-9604	177	19	run	run	VERB
elements-9604	177	20	on	on	ADP
elements-9604	177	21	1	1	NUM
elements-9604	177	22	%	%	NOUN
elements-9604	177	23	agarose	agarose	X
elements-9604	177	24	gel	gel	NOUN
elements-9604	177	25	.	.	PUNCT
elements-9604	178	1	the	the	DET
elements-9604	178	2	resulting	result	VERB
elements-9604	178	3	insert	insert	PROPN
elements-9604	178	4	vector	vector	NOUN
elements-9604	178	5	,	,	PUNCT
elements-9604	178	6	an	an	DET
elements-9604	178	7	scfv	scfv	NOUN
elements-9604	178	8	,	,	PUNCT
elements-9604	178	9	was	be	AUX
elements-9604	178	10	extracted	extract	VERB
elements-9604	178	11	from	from	ADP
elements-9604	178	12	the	the	DET
elements-9604	178	13	gel	gel	NOUN
elements-9604	178	14	.	.	PUNCT
elements-9604	179	1	the	the	DET
elements-9604	179	2	mammalian	mammalian	ADJ
elements-9604	179	3	expression	expression	NOUN
elements-9604	179	4	vector	vector	NOUN
elements-9604	179	5	pcep4	pcep4	NOUN
elements-9604	179	6	was	be	AUX
elements-9604	179	7	also	also	ADV
elements-9604	179	8	double	double	ADV
elements-9604	179	9	digested	digest	VERB
elements-9604	179	10	with	with	ADP
elements-9604	179	11	kpni	kpni	NOUN
elements-9604	179	12	and	and	CCONJ
elements-9604	179	13	sapi	sapi	NOUN
elements-9604	179	14	,	,	PUNCT
elements-9604	179	15	and	and	CCONJ
elements-9604	179	16	the	the	DET
elements-9604	179	17	double	double	ADJ
elements-9604	179	18	digest	digest	NOUN
elements-9604	179	19	products	product	NOUN
elements-9604	179	20	were	be	AUX
elements-9604	179	21	run	run	VERB
elements-9604	179	22	on	on	ADP
elements-9604	179	23	1	1	NUM
elements-9604	179	24	%	%	NOUN
elements-9604	179	25	agarose	agarose	X
elements-9604	179	26	gel	gel	NOUN
elements-9604	179	27	.	.	PUNCT
elements-9604	180	1	the	the	DET
elements-9604	180	2	resulting	result	VERB
elements-9604	180	3	backbone	backbone	NOUN
elements-9604	180	4	vector	vector	NOUN
elements-9604	180	5	was	be	AUX
elements-9604	180	6	extracted	extract	VERB
elements-9604	180	7	from	from	ADP
elements-9604	180	8	the	the	DET
elements-9604	180	9	gel	gel	NOUN
elements-9604	180	10	.	.	PUNCT
elements-9604	181	1	using	use	VERB
elements-9604	181	2	complementary	complementary	ADJ
elements-9604	181	3	“	"	PUNCT
elements-9604	181	4	sticky	sticky	ADJ
elements-9604	181	5	ends	end	NOUN
elements-9604	181	6	,	,	PUNCT
elements-9604	181	7	”	"	PUNCT
elements-9604	181	8	which	which	PRON
elements-9604	181	9	resulted	result	VERB
elements-9604	181	10	from	from	ADP
elements-9604	181	11	the	the	DET
elements-9604	181	12	double	double	ADJ
elements-9604	181	13	digest	digest	NOUN
elements-9604	181	14	,	,	PUNCT
elements-9604	181	15	the	the	DET
elements-9604	181	16	insert	insert	ADJ
elements-9604	181	17	vector	vector	NOUN
elements-9604	181	18	,	,	PUNCT
elements-9604	181	19	ll25x	ll25x	PROPN
elements-9604	181	20	,	,	PUNCT
elements-9604	181	21	was	be	AUX
elements-9604	181	22	effectively	effectively	ADV
elements-9604	181	23	ligated	ligate	VERB
elements-9604	181	24	into	into	ADP
elements-9604	181	25	the	the	DET
elements-9604	181	26	backbone	backbone	NOUN
elements-9604	181	27	vector	vector	NOUN
elements-9604	181	28	,	,	PUNCT
elements-9604	181	29	pcep4	pcep4	NOUN
elements-9604	181	30	.	.	PUNCT
elements-9604	182	1	top10f	top10f	NOUN
elements-9604	182	2	’	'	PUNCT
elements-9604	182	3	e.	e.	PROPN
elements-9604	182	4	coli	coli	PROPN
elements-9604	182	5	were	be	AUX
elements-9604	182	6	transformed	transform	VERB
elements-9604	182	7	with	with	ADP
elements-9604	182	8	the	the	DET
elements-9604	182	9	ligation	ligation	NOUN
elements-9604	182	10	product	product	NOUN
elements-9604	182	11	;	;	PUNCT
elements-9604	182	12	the	the	DET
elements-9604	182	13	1092	1092	NUM
elements-9604	182	14	bp	bp	PROPN
elements-9604	182	15	fragment	fragment	PROPN
elements-9604	182	16	was	be	AUX
elements-9604	182	17	cloned	clone	VERB
elements-9604	182	18	in	in	ADP
elements-9604	182	19	fusion	fusion	NOUN
elements-9604	182	20	with	with	ADP
elements-9604	182	21	the	the	DET
elements-9604	182	22	fc	fc	PROPN
elements-9604	182	23	region	region	PROPN
elements-9604	182	24	(	(	PUNCT
elements-9604	182	25	ch2	ch2	PROPN
elements-9604	182	26	+	+	CCONJ
elements-9604	182	27	ch3	ch3	PROPN
elements-9604	182	28	)	)	PUNCT
elements-9604	182	29	of	of	ADP
elements-9604	182	30	human	human	ADJ
elements-9604	182	31	igg1	igg1	NOUN
elements-9604	182	32	in	in	ADP
elements-9604	182	33	the	the	DET
elements-9604	182	34	mammalian	mammalian	ADJ
elements-9604	182	35	expression	expression	NOUN
elements-9604	182	36	vector	vector	NOUN
elements-9604	182	37	pcep4	pcep4	NOUN
elements-9604	182	38	.	.	PUNCT
elements-9604	183	1	these	these	DET
elements-9604	183	2	cells	cell	NOUN
elements-9604	183	3	,	,	PUNCT
elements-9604	183	4	containing	contain	VERB
elements-9604	183	5	the	the	DET
elements-9604	183	6	ligation	ligation	NOUN
elements-9604	183	7	product	product	NOUN
elements-9604	183	8	,	,	PUNCT
elements-9604	183	9	were	be	AUX
elements-9604	183	10	grown	grow	VERB
elements-9604	183	11	on	on	ADP
elements-9604	183	12	lb	lb	DET
elements-9604	183	13	agar	agar	NOUN
elements-9604	183	14	plates	plate	NOUN
elements-9604	183	15	containing	contain	VERB
elements-9604	183	16	ampicillin	ampicillin	NOUN
elements-9604	183	17	,	,	PUNCT
elements-9604	183	18	as	as	SCONJ
elements-9604	183	19	shown	show	VERB
elements-9604	183	20	in	in	ADP
elements-9604	183	21	figure	figure	NOUN
elements-9604	183	22	2b	2b	NOUN
elements-9604	183	23	.	.	PUNCT
elements-9604	184	1	a	a	DET
elements-9604	184	2	negative	negative	ADJ
elements-9604	184	3	background	background	NOUN
elements-9604	184	4	control	control	NOUN
elements-9604	184	5	with	with	ADP
elements-9604	184	6	e.	e.	PROPN
elements-9604	184	7	coli	coli	PROPN
elements-9604	184	8	76	76	NUM
elements-9604	184	9	elements	element	NOUN
elements-9604	184	10	:	:	PUNCT
elements-9604	184	11	:	:	PUNCT
elements-9604	184	12	spring	spring	NOUN
elements-9604	184	13	2017	2017	NUM
elements-9604	184	14	containing	contain	VERB
elements-9604	184	15	mammalian	mammalian	ADJ
elements-9604	184	16	expression	expression	NOUN
elements-9604	184	17	vector	vector	NOUN
elements-9604	184	18	pcep4	pcep4	NOUN
elements-9604	184	19	-	-	PUNCT
elements-9604	184	20	fc	fc	PROPN
elements-9604	184	21	-	-	PUNCT
elements-9604	184	22	ha	ha	INTJ
elements-9604	184	23	,	,	PUNCT
elements-9604	184	24	digested	digest	VERB
elements-9604	184	25	and	and	CCONJ
elements-9604	184	26	ligated	ligate	VERB
elements-9604	184	27	without	without	ADP
elements-9604	184	28	the	the	DET
elements-9604	184	29	ll25x	ll25x	PROPN
elements-9604	184	30	insert	insert	PROPN
elements-9604	184	31	vector	vector	PROPN
elements-9604	184	32	,	,	PUNCT
elements-9604	184	33	was	be	AUX
elements-9604	184	34	also	also	ADV
elements-9604	184	35	grown	grow	VERB
elements-9604	184	36	on	on	ADP
elements-9604	184	37	lb	lb	DET
elements-9604	184	38	agar	agar	NOUN
elements-9604	184	39	plates	plate	NOUN
elements-9604	184	40	containing	contain	VERB
elements-9604	184	41	ampicillin	ampicillin	NOUN
elements-9604	184	42	,	,	PUNCT
elements-9604	184	43	as	as	SCONJ
elements-9604	184	44	shown	show	VERB
elements-9604	184	45	in	in	ADP
elements-9604	184	46	figure	figure	NOUN
elements-9604	184	47	2a	2a	NUM
elements-9604	184	48	.	.	PUNCT
elements-9604	185	1	theoretically	theoretically	ADV
elements-9604	185	2	,	,	PUNCT
elements-9604	185	3	there	there	PRON
elements-9604	185	4	should	should	AUX
elements-9604	185	5	not	not	PART
elements-9604	185	6	be	be	AUX
elements-9604	185	7	any	any	DET
elements-9604	185	8	growth	growth	NOUN
elements-9604	185	9	on	on	ADP
elements-9604	185	10	the	the	DET
elements-9604	185	11	negative	negative	ADJ
elements-9604	185	12	control	control	NOUN
elements-9604	185	13	.	.	PUNCT
elements-9604	186	1	the	the	DET
elements-9604	186	2	vector	vector	NOUN
elements-9604	186	3	,	,	PUNCT
elements-9604	186	4	digested	digest	VERB
elements-9604	186	5	and	and	CCONJ
elements-9604	186	6	ligated	ligate	VERB
elements-9604	186	7	without	without	ADP
elements-9604	186	8	ll25x	ll25x	PROPN
elements-9604	186	9	,	,	PUNCT
elements-9604	186	10	would	would	AUX
elements-9604	186	11	not	not	PART
elements-9604	186	12	be	be	AUX
elements-9604	186	13	able	able	ADJ
elements-9604	186	14	to	to	PART
elements-9604	186	15	provide	provide	VERB
elements-9604	186	16	antibiotic	antibiotic	ADJ
elements-9604	186	17	resistance	resistance	NOUN
elements-9604	186	18	because	because	SCONJ
elements-9604	186	19	the	the	DET
elements-9604	186	20	replication	replication	NOUN
elements-9604	186	21	machinery	machinery	NOUN
elements-9604	186	22	of	of	ADP
elements-9604	186	23	the	the	DET
elements-9604	186	24	bacteria	bacteria	NOUN
elements-9604	186	25	would	would	AUX
elements-9604	186	26	be	be	AUX
elements-9604	186	27	affected	affect	VERB
elements-9604	186	28	,	,	PUNCT
elements-9604	186	29	preventing	prevent	VERB
elements-9604	186	30	the	the	DET
elements-9604	186	31	replication	replication	NOUN
elements-9604	186	32	of	of	ADP
elements-9604	186	33	the	the	DET
elements-9604	186	34	plasmid	plasmid	NOUN
elements-9604	186	35	.	.	PUNCT
elements-9604	187	1	since	since	SCONJ
elements-9604	187	2	the	the	DET
elements-9604	187	3	plasmid	plasmid	NOUN
elements-9604	187	4	is	be	AUX
elements-9604	187	5	not	not	PART
elements-9604	187	6	replicated	replicate	VERB
elements-9604	187	7	,	,	PUNCT
elements-9604	187	8	the	the	DET
elements-9604	187	9	ampicillin	ampicillin	PROPN
elements-9604	187	10	resistance	resistance	NOUN
elements-9604	187	11	gene	gene	NOUN
elements-9604	187	12	can	can	AUX
elements-9604	187	13	not	not	PART
elements-9604	187	14	be	be	AUX
elements-9604	187	15	expressed	express	VERB
elements-9604	187	16	.	.	PUNCT
elements-9604	188	1	however	however	ADV
elements-9604	188	2	,	,	PUNCT
elements-9604	188	3	the	the	DET
elements-9604	188	4	growth	growth	NOUN
elements-9604	188	5	of	of	ADP
elements-9604	188	6	a	a	DET
elements-9604	188	7	few	few	ADJ
elements-9604	188	8	colonies	colony	NOUN
elements-9604	188	9	is	be	AUX
elements-9604	188	10	clearly	clearly	ADV
elements-9604	188	11	visible	visible	ADJ
elements-9604	188	12	.	.	PUNCT
elements-9604	189	1	this	this	DET
elements-9604	189	2	incidence	incidence	NOUN
elements-9604	189	3	may	may	AUX
elements-9604	189	4	be	be	AUX
elements-9604	189	5	due	due	ADJ
elements-9604	189	6	to	to	ADP
elements-9604	189	7	some	some	PRON
elements-9604	189	8	of	of	ADP
elements-9604	189	9	the	the	DET
elements-9604	189	10	pcep4	pcep4	NOUN
elements-9604	189	11	not	not	PART
elements-9604	189	12	being	be	AUX
elements-9604	189	13	completely	completely	ADV
elements-9604	189	14	digested	digest	VERB
elements-9604	189	15	,	,	PUNCT
elements-9604	189	16	resulting	result	VERB
elements-9604	189	17	in	in	ADP
elements-9604	189	18	a	a	DET
elements-9604	189	19	functional	functional	ADJ
elements-9604	189	20	ampicillin	ampicillin	NOUN
elements-9604	189	21	resistance	resistance	NOUN
elements-9604	189	22	gene	gene	NOUN
elements-9604	189	23	.	.	PUNCT
elements-9604	190	1	there	there	PRON
elements-9604	190	2	is	be	VERB
elements-9604	190	3	an	an	DET
elements-9604	190	4	abundance	abundance	NOUN
elements-9604	190	5	of	of	ADP
elements-9604	190	6	colonies	colony	NOUN
elements-9604	190	7	on	on	ADP
elements-9604	190	8	the	the	DET
elements-9604	190	9	plate	plate	NOUN
elements-9604	190	10	with	with	ADP
elements-9604	190	11	the	the	DET
elements-9604	190	12	bacteria	bacteria	NOUN
elements-9604	190	13	that	that	PRON
elements-9604	190	14	contain	contain	VERB
elements-9604	190	15	the	the	DET
elements-9604	190	16	ligation	ligation	NOUN
elements-9604	190	17	product	product	NOUN
elements-9604	190	18	.	.	PUNCT
elements-9604	191	1	the	the	DET
elements-9604	191	2	ligation	ligation	NOUN
elements-9604	191	3	of	of	ADP
elements-9604	191	4	ll25x	ll25x	NOUN
elements-9604	191	5	to	to	ADP
elements-9604	191	6	the	the	DET
elements-9604	191	7	backbone	backbone	NOUN
elements-9604	191	8	vector	vector	NOUN
elements-9604	191	9	pcep4	pcep4	NOUN
elements-9604	191	10	allows	allow	VERB
elements-9604	191	11	the	the	DET
elements-9604	191	12	cell	cell	NOUN
elements-9604	191	13	to	to	PART
elements-9604	191	14	replicate	replicate	VERB
elements-9604	191	15	,	,	PUNCT
elements-9604	191	16	resulting	result	VERB
elements-9604	191	17	in	in	ADP
elements-9604	191	18	the	the	DET
elements-9604	191	19	expression	expression	NOUN
elements-9604	191	20	of	of	ADP
elements-9604	191	21	the	the	DET
elements-9604	191	22	ampicillin	ampicillin	PROPN
elements-9604	191	23	resistance	resistance	NOUN
elements-9604	191	24	gene	gene	NOUN
elements-9604	191	25	.	.	PUNCT
elements-9604	192	1	the	the	DET
elements-9604	192	2	greater	great	ADJ
elements-9604	192	3	amount	amount	NOUN
elements-9604	192	4	of	of	ADP
elements-9604	192	5	colonies	colony	NOUN
elements-9604	192	6	present	present	ADJ
elements-9604	192	7	on	on	ADP
elements-9604	192	8	the	the	DET
elements-9604	192	9	plate	plate	NOUN
elements-9604	192	10	with	with	ADP
elements-9604	192	11	the	the	DET
elements-9604	192	12	bacteria	bacteria	NOUN
elements-9604	192	13	containing	contain	VERB
elements-9604	192	14	the	the	DET
elements-9604	192	15	ligation	ligation	NOUN
elements-9604	192	16	product	product	NOUN
elements-9604	192	17	compared	compare	VERB
elements-9604	192	18	to	to	ADP
elements-9604	192	19	the	the	DET
elements-9604	192	20	plate	plate	NOUN
elements-9604	192	21	with	with	ADP
elements-9604	192	22	the	the	DET
elements-9604	192	23	bacteria	bacteria	NOUN
elements-9604	192	24	containing	contain	VERB
elements-9604	192	25	pcep4	pcep4	NOUN
elements-9604	192	26	,	,	PUNCT
elements-9604	192	27	digested	digest	VERB
elements-9604	192	28	and	and	CCONJ
elements-9604	192	29	ligated	ligate	VERB
elements-9604	192	30	without	without	ADP
elements-9604	192	31	ll25x	ll25x	PROPN
elements-9604	192	32	,	,	PUNCT
elements-9604	192	33	suggests	suggest	VERB
elements-9604	192	34	a	a	DET
elements-9604	192	35	successful	successful	ADJ
elements-9604	192	36	ligation	ligation	NOUN
elements-9604	192	37	reaction	reaction	NOUN
elements-9604	192	38	.	.	PUNCT
elements-9604	193	1	a	a	DET
elements-9604	193	2	colony	colony	NOUN
elements-9604	193	3	,	,	PUNCT
elements-9604	193	4	corresponding	correspond	VERB
elements-9604	193	5	to	to	ADP
elements-9604	193	6	the	the	DET
elements-9604	193	7	bacteria	bacteria	NOUN
elements-9604	193	8	transformed	transform	VERB
elements-9604	193	9	with	with	ADP
elements-9604	193	10	the	the	DET
elements-9604	193	11	pcep4	pcep4	NOUN
elements-9604	193	12	/	/	SYM
elements-9604	193	13	ll25x	ll25x	NOUN
elements-9604	193	14	ligation	ligation	NOUN
elements-9604	193	15	product	product	NOUN
elements-9604	193	16	,	,	PUNCT
elements-9604	193	17	was	be	AUX
elements-9604	193	18	selected	select	VERB
elements-9604	193	19	,	,	PUNCT
elements-9604	193	20	and	and	CCONJ
elements-9604	193	21	the	the	DET
elements-9604	193	22	pcep4	pcep4	NOUN
elements-9604	193	23	/	/	SYM
elements-9604	193	24	ll25x	ll25x	PROPN
elements-9604	193	25	dna	dna	PROPN
elements-9604	193	26	from	from	ADP
elements-9604	193	27	the	the	DET
elements-9604	193	28	colony	colony	NOUN
elements-9604	193	29	was	be	AUX
elements-9604	193	30	extracted	extract	VERB
elements-9604	193	31	via	via	ADP
elements-9604	193	32	dna	dna	PROPN
elements-9604	193	33	mini	mini	NOUN
elements-9604	193	34	-	-	NOUN
elements-9604	193	35	prep	prep	NOUN
elements-9604	193	36	.	.	PUNCT
elements-9604	194	1	the	the	DET
elements-9604	194	2	pcep4	pcep4	PROPN
elements-9604	194	3	/	/	SYM
elements-9604	194	4	ll25x	ll25x	NOUN
elements-9604	194	5	dna	dna	PROPN
elements-9604	194	6	and	and	CCONJ
elements-9604	194	7	the	the	DET
elements-9604	194	8	pcep4	pcep4	NOUN
elements-9604	194	9	-	-	PUNCT
elements-9604	194	10	fc	fc	PROPN
elements-9604	194	11	-	-	PUNCT
elements-9604	194	12	ha	ha	INTJ
elements-9604	194	13	dna	dna	PROPN
elements-9604	194	14	,	,	PUNCT
elements-9604	194	15	used	use	VERB
elements-9604	194	16	as	as	ADP
elements-9604	194	17	a	a	DET
elements-9604	194	18	control	control	NOUN
elements-9604	194	19	,	,	PUNCT
elements-9604	194	20	were	be	AUX
elements-9604	194	21	double	double	ADV
elements-9604	194	22	digested	digest	VERB
elements-9604	194	23	with	with	ADP
elements-9604	194	24	kpni	kpni	NOUN
elements-9604	194	25	-	-	PUNCT
elements-9604	194	26	hf/	hf/	NOUN
elements-9604	194	27	sapi	sapi	NOUN
elements-9604	194	28	and	and	CCONJ
elements-9604	194	29	kpni	kpni	NOUN
elements-9604	194	30	/	/	SYM
elements-9604	194	31	bamhi	bamhi	NOUN
elements-9604	194	32	-	-	PUNCT
elements-9604	194	33	hf	hf	NOUN
elements-9604	194	34	.	.	PUNCT
elements-9604	195	1	the	the	DET
elements-9604	195	2	products	product	NOUN
elements-9604	195	3	of	of	ADP
elements-9604	195	4	the	the	DET
elements-9604	195	5	double	double	ADJ
elements-9604	195	6	digests	digest	NOUN
elements-9604	195	7	were	be	AUX
elements-9604	195	8	run	run	VERB
elements-9604	195	9	on	on	ADP
elements-9604	195	10	1	1	NUM
elements-9604	195	11	%	%	NOUN
elements-9604	195	12	agarose	agarose	X
elements-9604	195	13	gel	gel	NOUN
elements-9604	195	14	.	.	PUNCT
elements-9604	196	1	the	the	DET
elements-9604	196	2	pcep4	pcep4	PROPN
elements-9604	196	3	/	/	SYM
elements-9604	196	4	ll25x	ll25x	PROPN
elements-9604	196	5	dna	dna	PROPN
elements-9604	196	6	double	double	ADV
elements-9604	196	7	digested	digest	VERB
elements-9604	196	8	with	with	ADP
elements-9604	196	9	kpni	kpni	NOUN
elements-9604	196	10	-	-	PUNCT
elements-9604	196	11	hf	hf	NOUN
elements-9604	196	12	and	and	CCONJ
elements-9604	196	13	sapi	sapi	NOUN
elements-9604	196	14	produced	produce	VERB
elements-9604	196	15	a	a	DET
elements-9604	196	16	band	band	NOUN
elements-9604	196	17	at	at	ADP
elements-9604	196	18	approximately	approximately	ADV
elements-9604	196	19	1092	1092	NUM
elements-9604	196	20	bp	bp	NOUN
elements-9604	196	21	,	,	PUNCT
elements-9604	196	22	and	and	CCONJ
elements-9604	196	23	the	the	DET
elements-9604	196	24	pcep4	pcep4	NOUN
elements-9604	196	25	-	-	PUNCT
elements-9604	196	26	fc	fc	PROPN
elements-9604	196	27	-	-	PUNCT
elements-9604	196	28	ha	ha	INTJ
elements-9604	196	29	dna	dna	PROPN
elements-9604	196	30	double	double	ADV
elements-9604	196	31	digested	digest	VERB
elements-9604	196	32	with	with	ADP
elements-9604	196	33	kpni	kpni	NOUN
elements-9604	196	34	-	-	PUNCT
elements-9604	196	35	hf	hf	NOUN
elements-9604	196	36	and	and	CCONJ
elements-9604	196	37	sapi	sapi	NOUN
elements-9604	196	38	also	also	ADV
elements-9604	196	39	produced	produce	VERB
elements-9604	196	40	a	a	DET
elements-9604	196	41	band	band	NOUN
elements-9604	196	42	at	at	ADP
elements-9604	196	43	approximately	approximately	ADV
elements-9604	196	44	1092	1092	NUM
elements-9604	196	45	bp	bp	NOUN
elements-9604	196	46	.	.	PUNCT
elements-9604	197	1	the	the	DET
elements-9604	197	2	double	double	ADJ
elements-9604	197	3	digested	digest	VERB
elements-9604	197	4	dna	dna	NOUN
elements-9604	197	5	fragments	fragment	NOUN
elements-9604	197	6	were	be	AUX
elements-9604	197	7	similar	similar	ADJ
elements-9604	197	8	in	in	ADP
elements-9604	197	9	size	size	NOUN
elements-9604	197	10	;	;	PUNCT
elements-9604	197	11	therefore	therefore	ADV
elements-9604	197	12	,	,	PUNCT
elements-9604	197	13	it	it	PRON
elements-9604	197	14	could	could	AUX
elements-9604	197	15	not	not	PART
elements-9604	197	16	be	be	AUX
elements-9604	197	17	verified	verify	VERB
elements-9604	197	18	that	that	SCONJ
elements-9604	197	19	the	the	DET
elements-9604	197	20	ligation	ligation	NOUN
elements-9604	197	21	product	product	NOUN
elements-9604	197	22	contained	contain	VERB
elements-9604	197	23	the	the	DET
elements-9604	197	24	ll25x	ll25x	PROPN
elements-9604	197	25	dna	dna	PROPN
elements-9604	197	26	based	base	VERB
elements-9604	197	27	on	on	ADP
elements-9604	197	28	this	this	DET
elements-9604	197	29	result	result	NOUN
elements-9604	197	30	alone	alone	ADV
elements-9604	197	31	.	.	PUNCT
elements-9604	198	1	when	when	SCONJ
elements-9604	198	2	double	double	ADJ
elements-9604	198	3	digested	digest	VERB
elements-9604	198	4	with	with	ADP
elements-9604	198	5	kpni	kpni	PROPN
elements-9604	198	6	and	and	CCONJ
elements-9604	198	7	bamhi	bamhi	NOUN
elements-9604	198	8	-	-	PUNCT
elements-9604	198	9	hf	hf	NOUN
elements-9604	198	10	,	,	PUNCT
elements-9604	198	11	pcep4	pcep4	ADJ
elements-9604	198	12	/	/	SYM
elements-9604	198	13	ll25x	ll25x	NOUN
elements-9604	198	14	dna	dna	PROPN
elements-9604	198	15	and	and	CCONJ
elements-9604	198	16	pcep4	pcep4	NOUN
elements-9604	198	17	-	-	PUNCT
elements-9604	198	18	fc	fc	VERB
elements-9604	198	19	-	-	PUNCT
elements-9604	198	20	ha	ha	INTJ
elements-9604	198	21	dna	dna	PROPN
elements-9604	198	22	resulted	result	VERB
elements-9604	198	23	in	in	ADP
elements-9604	198	24	bands	band	NOUN
elements-9604	198	25	with	with	ADP
elements-9604	198	26	different	different	ADJ
elements-9604	198	27	sizes	size	NOUN
elements-9604	198	28	.	.	PUNCT
elements-9604	199	1	this	this	PRON
elements-9604	199	2	is	be	AUX
elements-9604	199	3	due	due	ADJ
elements-9604	199	4	to	to	ADP
elements-9604	199	5	the	the	DET
elements-9604	199	6	bamhi	bamhi	ADJ
elements-9604	199	7	restriction	restriction	NOUN
elements-9604	199	8	site	site	NOUN
elements-9604	199	9	residing	reside	VERB
elements-9604	199	10	in	in	ADP
elements-9604	199	11	different	different	ADJ
elements-9604	199	12	locations	location	NOUN
elements-9604	199	13	between	between	ADP
elements-9604	199	14	the	the	DET
elements-9604	199	15	two	two	NUM
elements-9604	199	16	vectors	vector	NOUN
elements-9604	199	17	.	.	PUNCT
elements-9604	200	1	the	the	DET
elements-9604	200	2	pcep4	pcep4	NOUN
elements-9604	200	3	/	/	SYM
elements-9604	200	4	ll25x	ll25x	NOUN
elements-9604	200	5	contains	contain	VERB
elements-9604	200	6	a	a	DET
elements-9604	200	7	bamhi	bamhi	NOUN
elements-9604	200	8	restriction	restriction	NOUN
elements-9604	200	9	site	site	NOUN
elements-9604	200	10	that	that	PRON
elements-9604	200	11	appears	appear	VERB
elements-9604	200	12	before	before	ADP
elements-9604	200	13	sapi	sapi	NOUN
elements-9604	200	14	,	,	PUNCT
elements-9604	200	15	and	and	CCONJ
elements-9604	200	16	the	the	DET
elements-9604	200	17	pcep4	pcep4	NOUN
elements-9604	200	18	-	-	PUNCT
elements-9604	200	19	fc	fc	VERB
elements-9604	200	20	-	-	PUNCT
elements-9604	200	21	ha	ha	INTJ
elements-9604	200	22	dna	dna	PROPN
elements-9604	200	23	contains	contain	VERB
elements-9604	200	24	a	a	DET
elements-9604	200	25	bamhi	bamhi	NOUN
elements-9604	200	26	restriction	restriction	NOUN
elements-9604	200	27	site	site	NOUN
elements-9604	200	28	that	that	PRON
elements-9604	200	29	appears	appear	VERB
elements-9604	200	30	after	after	ADP
elements-9604	200	31	sapi	sapi	NOUN
elements-9604	200	32	;	;	PUNCT
elements-9604	200	33	therefore	therefore	ADV
elements-9604	200	34	,	,	PUNCT
elements-9604	200	35	the	the	DET
elements-9604	200	36	double	double	ADJ
elements-9604	200	37	digested	digest	VERB
elements-9604	200	38	pcep4	pcep4	NOUN
elements-9604	200	39	/	/	SYM
elements-9604	200	40	ll25x	ll25x	PROPN
elements-9604	200	41	dna	dna	PROPN
elements-9604	200	42	will	will	AUX
elements-9604	200	43	result	result	VERB
elements-9604	200	44	in	in	ADP
elements-9604	200	45	a	a	DET
elements-9604	200	46	smaller	small	ADJ
elements-9604	200	47	fragment	fragment	NOUN
elements-9604	200	48	than	than	ADP
elements-9604	200	49	the	the	DET
elements-9604	200	50	fragment	fragment	NOUN
elements-9604	200	51	produced	produce	VERB
elements-9604	200	52	by	by	ADP
elements-9604	200	53	the	the	DET
elements-9604	200	54	double	double	ADJ
elements-9604	200	55	digested	digested	ADJ
elements-9604	200	56	pcep4	pcep4	NOUN
elements-9604	200	57	-	-	PUNCT
elements-9604	200	58	fcha	fcha	NOUN
elements-9604	200	59	dna	dna	NOUN
elements-9604	200	60	.	.	PUNCT
elements-9604	201	1	this	this	DET
elements-9604	201	2	result	result	NOUN
elements-9604	201	3	verified	verify	VERB
elements-9604	201	4	that	that	SCONJ
elements-9604	201	5	the	the	DET
elements-9604	201	6	ligation	ligation	NOUN
elements-9604	201	7	product	product	NOUN
elements-9604	201	8	contained	contain	VERB
elements-9604	201	9	the	the	DET
elements-9604	201	10	ll25x	ll25x	PROPN
elements-9604	201	11	dna	dna	PROPN
elements-9604	201	12	.	.	PUNCT
elements-9604	202	1	after	after	ADP
elements-9604	202	2	identifying	identify	VERB
elements-9604	202	3	three	three	NUM
elements-9604	202	4	positive	positive	ADJ
elements-9604	202	5	ll25x	ll25x	NOUN
elements-9604	202	6	clones	clone	NOUN
elements-9604	202	7	via	via	ADP
elements-9604	202	8	double	double	ADJ
elements-9604	202	9	digestion	digestion	NOUN
elements-9604	202	10	,	,	PUNCT
elements-9604	202	11	the	the	DET
elements-9604	202	12	clones	clone	NOUN
elements-9604	202	13	were	be	AUX
elements-9604	202	14	sent	send	VERB
elements-9604	202	15	to	to	ADP
elements-9604	202	16	eton	eton	PROPN
elements-9604	202	17	bioscience	bioscience	PROPN
elements-9604	202	18	for	for	ADP
elements-9604	202	19	sanger	sanger	PROPN
elements-9604	202	20	sequencing	sequencing	NOUN
elements-9604	202	21	.	.	PUNCT
elements-9604	203	1	once	once	SCONJ
elements-9604	203	2	the	the	DET
elements-9604	203	3	dna	dna	PROPN
elements-9604	203	4	sequence	sequence	NOUN
elements-9604	203	5	results	result	NOUN
elements-9604	203	6	were	be	AUX
elements-9604	203	7	received	receive	VERB
elements-9604	203	8	,	,	PUNCT
elements-9604	203	9	the	the	DET
elements-9604	203	10	software	software	NOUN
elements-9604	203	11	geneious	geneious	ADJ
elements-9604	203	12	was	be	AUX
elements-9604	203	13	used	use	VERB
elements-9604	203	14	to	to	PART
elements-9604	203	15	analyze	analyze	VERB
elements-9604	203	16	the	the	DET
elements-9604	203	17	sequence	sequence	NOUN
elements-9604	203	18	of	of	ADP
elements-9604	203	19	ll25x	ll25x	PROPN
elements-9604	203	20	.	.	PUNCT
elements-9604	204	1	the	the	DET
elements-9604	204	2	sequence	sequence	NOUN
elements-9604	204	3	analysis	analysis	NOUN
elements-9604	204	4	confirmed	confirm	VERB
elements-9604	204	5	that	that	SCONJ
elements-9604	204	6	the	the	DET
elements-9604	204	7	correct	correct	ADJ
elements-9604	204	8	ll25x	ll25x	NOUN
elements-9604	204	9	sequence	sequence	NOUN
elements-9604	204	10	had	have	AUX
elements-9604	204	11	been	be	AUX
elements-9604	204	12	cloned	clone	VERB
elements-9604	204	13	into	into	ADP
elements-9604	204	14	the	the	DET
elements-9604	204	15	mammalian	mammalian	ADJ
elements-9604	204	16	expression	expression	NOUN
elements-9604	204	17	vector	vector	NOUN
elements-9604	204	18	pcep4	pcep4	NOUN
elements-9604	204	19	.	.	PUNCT
elements-9604	205	1	the	the	DET
elements-9604	205	2	sequences	sequence	NOUN
elements-9604	205	3	of	of	ADP
elements-9604	205	4	all	all	DET
elements-9604	205	5	three	three	NUM
elements-9604	205	6	ll25x	ll25x	NOUN
elements-9604	205	7	clones	clone	NOUN
elements-9604	205	8	were	be	AUX
elements-9604	205	9	aligned	align	VERB
elements-9604	205	10	identically	identically	ADV
elements-9604	205	11	to	to	ADP
elements-9604	205	12	the	the	DET
elements-9604	205	13	original	original	ADJ
elements-9604	205	14	codon	codon	NOUN
elements-9604	205	15	-	-	PUNCT
elements-9604	205	16	optimized	optimize	VERB
elements-9604	205	17	version	version	NOUN
elements-9604	205	18	of	of	ADP
elements-9604	205	19	the	the	DET
elements-9604	205	20	ll25x	ll25x	NOUN
elements-9604	205	21	sequence	sequence	NOUN
elements-9604	205	22	,	,	PUNCT
elements-9604	205	23	as	as	SCONJ
elements-9604	205	24	shown	show	VERB
elements-9604	205	25	in	in	ADP
elements-9604	205	26	figure	figure	NOUN
elements-9604	205	27	3	3	NUM
elements-9604	205	28	.	.	PUNCT
elements-9604	206	1	a	a	DET
elements-9604	206	2	positive	positive	ADJ
elements-9604	206	3	ll25x	ll25x	NOUN
elements-9604	206	4	clone	clone	NOUN
elements-9604	206	5	was	be	AUX
elements-9604	206	6	also	also	ADV
elements-9604	206	7	selected	select	VERB
elements-9604	206	8	to	to	PART
elements-9604	206	9	be	be	AUX
elements-9604	206	10	cultured	cultured	ADJ
elements-9604	206	11	in	in	ADP
elements-9604	206	12	a	a	DET
elements-9604	206	13	tube	tube	NOUN
elements-9604	206	14	containing	contain	VERB
elements-9604	206	15	3	3	NUM
elements-9604	206	16	ml	ml	NOUN
elements-9604	206	17	of	of	ADP
elements-9604	206	18	lb	lb	PRON
elements-9604	206	19	/	/	SYM
elements-9604	206	20	ampicillin	ampicillin	NOUN
elements-9604	206	21	media	medium	NOUN
elements-9604	206	22	,	,	PUNCT
elements-9604	206	23	and	and	CCONJ
elements-9604	206	24	the	the	DET
elements-9604	206	25	resulting	result	VERB
elements-9604	206	26	pcpe4	pcpe4	X
elements-9604	206	27	/	/	SYM
elements-9604	206	28	ll25x	ll25x	PROPN
elements-9604	206	29	dna	dna	PROPN
elements-9604	206	30	from	from	ADP
elements-9604	206	31	the	the	DET
elements-9604	206	32	bacteria	bacteria	NOUN
elements-9604	206	33	was	be	AUX
elements-9604	206	34	extracted	extract	VERB
elements-9604	206	35	via	via	ADP
elements-9604	206	36	dna	dna	PROPN
elements-9604	206	37	maxi	maxi	NOUN
elements-9604	206	38	-	-	PUNCT
elements-9604	206	39	prep	prep	NOUN
elements-9604	206	40	.	.	PUNCT
elements-9604	207	1	the	the	DET
elements-9604	207	2	extracted	extract	VERB
elements-9604	207	3	pcep4	pcep4	NOUN
elements-9604	207	4	/	/	SYM
elements-9604	207	5	ll25x	ll25x	PROPN
elements-9604	207	6	dna	dna	PROPN
elements-9604	207	7	was	be	AUX
elements-9604	207	8	used	use	VERB
elements-9604	207	9	to	to	PART
elements-9604	207	10	transfect	transfect	VERB
elements-9604	207	11	293	293	NUM
elements-9604	207	12	t	t	NOUN
elements-9604	207	13	cells	cell	NOUN
elements-9604	207	14	.	.	PUNCT
elements-9604	208	1	cleavage	cleavage	NOUN
elements-9604	208	2	of	of	ADP
elements-9604	208	3	sivmac239gp140	sivmac239gp140	PROPN
elements-9604	208	4	into	into	ADP
elements-9604	208	5	monomeric	monomeric	ADJ
elements-9604	208	6	surface	surface	NOUN
elements-9604	208	7	subunit	subunit	NOUN
elements-9604	208	8	sivmac239gp120	sivmac239gp120	PROPN
elements-9604	208	9	and	and	CCONJ
elements-9604	208	10	ectodomain	ectodomain	VERB
elements-9604	208	11	sivmac239gp41	sivmac239gp41	PROPN
elements-9604	208	12	occurred	occur	VERB
elements-9604	208	13	once	once	SCONJ
elements-9604	208	14	the	the	DET
elements-9604	208	15	proteins	protein	NOUN
elements-9604	208	16	were	be	AUX
elements-9604	208	17	expressed	express	VERB
elements-9604	208	18	in	in	ADP
elements-9604	208	19	the	the	DET
elements-9604	208	20	293	293	NUM
elements-9604	208	21	t	t	NOUN
elements-9604	208	22	cells	cell	NOUN
elements-9604	208	23	.	.	PUNCT
elements-9604	209	1	five	five	NUM
elements-9604	209	2	days	day	NOUN
elements-9604	209	3	after	after	ADP
elements-9604	209	4	the	the	DET
elements-9604	209	5	transfection	transfection	NOUN
elements-9604	209	6	,	,	PUNCT
elements-9604	209	7	the	the	DET
elements-9604	209	8	culture	culture	NOUN
elements-9604	209	9	supernatant	supernatant	NOUN
elements-9604	209	10	was	be	AUX
elements-9604	209	11	centrifuged	centrifuge	VERB
elements-9604	209	12	to	to	PART
elements-9604	209	13	remove	remove	VERB
elements-9604	209	14	the	the	DET
elements-9604	209	15	cell	cell	NOUN
elements-9604	209	16	debris	debris	NOUN
elements-9604	209	17	,	,	PUNCT
elements-9604	209	18	and	and	CCONJ
elements-9604	209	19	the	the	DET
elements-9604	209	20	supernatant	supernatant	NOUN
elements-9604	209	21	was	be	AUX
elements-9604	209	22	transferred	transfer	VERB
elements-9604	209	23	to	to	ADP
elements-9604	209	24	new	new	ADJ
elements-9604	209	25	tubes	tube	NOUN
elements-9604	209	26	.	.	PUNCT
elements-9604	210	1	the	the	DET
elements-9604	210	2	proteins	protein	NOUN
elements-9604	210	3	in	in	ADP
elements-9604	210	4	the	the	DET
elements-9604	210	5	supernatant	supernatant	NOUN
elements-9604	210	6	77	77	NUM
elements-9604	210	7	the	the	DET
elements-9604	210	8	road	road	NOUN
elements-9604	210	9	to	to	ADP
elements-9604	210	10	a	a	DET
elements-9604	210	11	cure	cure	NOUN
elements-9604	210	12	were	be	AUX
elements-9604	210	13	isolated	isolate	VERB
elements-9604	210	14	via	via	ADP
elements-9604	210	15	protein	protein	NOUN
elements-9604	210	16	a	a	DET
elements-9604	210	17	-	-	PUNCT
elements-9604	210	18	agarose	agarose	NOUN
elements-9604	210	19	beads	bead	NOUN
elements-9604	210	20	and	and	CCONJ
elements-9604	210	21	dialysis	dialysis	NOUN
elements-9604	210	22	,	,	PUNCT
elements-9604	210	23	and	and	CCONJ
elements-9604	210	24	elisa	elisa	PROPN
elements-9604	210	25	was	be	AUX
elements-9604	210	26	performed	perform	VERB
elements-9604	210	27	,	,	PUNCT
elements-9604	210	28	as	as	SCONJ
elements-9604	210	29	shown	show	VERB
elements-9604	210	30	in	in	ADP
elements-9604	210	31	figure	figure	NOUN
elements-9604	210	32	4	4	NUM
elements-9604	210	33	.	.	PUNCT
elements-9604	211	1	the	the	DET
elements-9604	211	2	binding	bind	VERB
elements-9604	211	3	specificity	specificity	NOUN
elements-9604	211	4	of	of	ADP
elements-9604	211	5	antibodies	antibody	NOUN
elements-9604	211	6	,	,	PUNCT
elements-9604	211	7	su24	su24	PROPN
elements-9604	211	8	,	,	PUNCT
elements-9604	211	9	su343	su343	PROPN
elements-9604	211	10	,	,	PUNCT
elements-9604	211	11	and	and	CCONJ
elements-9604	211	12	ll25x	ll25x	NOUN
elements-9604	211	13	,	,	PUNCT
elements-9604	211	14	against	against	ADP
elements-9604	211	15	antigens	antigen	NOUN
elements-9604	211	16	,	,	PUNCT
elements-9604	211	17	sivmac239gp140	sivmac239gp140	PROPN
elements-9604	211	18	,	,	PUNCT
elements-9604	211	19	sivmac239gp120	sivmac239gp120	NOUN
elements-9604	211	20	,	,	PUNCT
elements-9604	211	21	and	and	CCONJ
elements-9604	211	22	bsa	bsa	PROPN
elements-9604	211	23	(	(	PUNCT
elements-9604	211	24	bovine	bovine	NOUN
elements-9604	211	25	serum	serum	NOUN
elements-9604	211	26	albumin	albumin	NOUN
elements-9604	211	27	–	–	PUNCT
elements-9604	211	28	used	use	VERB
elements-9604	211	29	as	as	ADP
elements-9604	211	30	a	a	DET
elements-9604	211	31	control	control	NOUN
elements-9604	211	32	)	)	PUNCT
elements-9604	211	33	,	,	PUNCT
elements-9604	211	34	was	be	AUX
elements-9604	211	35	analyzed	analyze	VERB
elements-9604	211	36	.	.	PUNCT
elements-9604	212	1	su24	su24	PROPN
elements-9604	212	2	,	,	PUNCT
elements-9604	212	3	su343	su343	PROPN
elements-9604	212	4	,	,	PUNCT
elements-9604	212	5	and	and	CCONJ
elements-9604	212	6	ll25x	ll25x	NOUN
elements-9604	212	7	were	be	AUX
elements-9604	212	8	used	use	VERB
elements-9604	212	9	as	as	ADP
elements-9604	212	10	primary	primary	ADJ
elements-9604	212	11	antibodies	antibody	NOUN
elements-9604	212	12	,	,	PUNCT
elements-9604	212	13	and	and	CCONJ
elements-9604	212	14	anti	anti	ADJ
elements-9604	212	15	-	-	ADJ
elements-9604	212	16	human	human	ADJ
elements-9604	212	17	igg	igg	PROPN
elements-9604	212	18	-	-	PUNCT
elements-9604	212	19	hrp	hrp	PROPN
elements-9604	212	20	was	be	AUX
elements-9604	212	21	used	use	VERB
elements-9604	212	22	as	as	ADP
elements-9604	212	23	a	a	DET
elements-9604	212	24	secondary	secondary	ADJ
elements-9604	212	25	antibody	antibody	NOUN
elements-9604	212	26	.	.	PUNCT
elements-9604	213	1	tmb	tmb	NOUN
elements-9604	213	2	solution	solution	NOUN
elements-9604	213	3	was	be	AUX
elements-9604	213	4	used	use	VERB
elements-9604	213	5	for	for	ADP
elements-9604	213	6	detection	detection	NOUN
elements-9604	213	7	,	,	PUNCT
elements-9604	213	8	and	and	CCONJ
elements-9604	213	9	the	the	DET
elements-9604	213	10	plate	plate	NOUN
elements-9604	213	11	was	be	AUX
elements-9604	213	12	read	read	VERB
elements-9604	213	13	at	at	ADP
elements-9604	213	14	450	450	NUM
elements-9604	213	15	nm	nm	NOUN
elements-9604	213	16	.	.	PUNCT
elements-9604	214	1	su24	su24	PROPN
elements-9604	214	2	,	,	PUNCT
elements-9604	214	3	su343	su343	PROPN
elements-9604	214	4	,	,	PUNCT
elements-9604	214	5	and	and	CCONJ
elements-9604	214	6	ll25x	ll25x	NOUN
elements-9604	214	7	did	do	AUX
elements-9604	214	8	not	not	PART
elements-9604	214	9	bind	bind	VERB
elements-9604	214	10	to	to	ADP
elements-9604	214	11	bsa	bsa	PROPN
elements-9604	214	12	,	,	PUNCT
elements-9604	214	13	which	which	PRON
elements-9604	214	14	was	be	AUX
elements-9604	214	15	expected	expect	VERB
elements-9604	214	16	.	.	PUNCT
elements-9604	215	1	su24	su24	PROPN
elements-9604	215	2	and	and	CCONJ
elements-9604	215	3	su343	su343	PROPN
elements-9604	215	4	bound	bind	VERB
elements-9604	215	5	to	to	ADP
elements-9604	215	6	the	the	DET
elements-9604	215	7	envelope	envelope	NOUN
elements-9604	215	8	proteins	protein	NOUN
elements-9604	215	9	gp140	gp140	PROPN
elements-9604	215	10	and	and	CCONJ
elements-9604	215	11	gp120	gp120	PROPN
elements-9604	215	12	;	;	PUNCT
elements-9604	215	13	however	however	ADV
elements-9604	215	14	,	,	PUNCT
elements-9604	215	15	ll25x	ll25x	PROPN
elements-9604	215	16	bound	bind	VERB
elements-9604	215	17	to	to	ADP
elements-9604	215	18	gp140	gp140	PROPN
elements-9604	215	19	but	but	CCONJ
elements-9604	215	20	not	not	PART
elements-9604	215	21	gp120	gp120	PROPN
elements-9604	215	22	.	.	PUNCT
elements-9604	216	1	elisa	elisa	PROPN
elements-9604	216	2	determines	determine	VERB
elements-9604	216	3	the	the	DET
elements-9604	216	4	binding	bind	VERB
elements-9604	216	5	specificities	specificity	NOUN
elements-9604	216	6	of	of	ADP
elements-9604	216	7	antibodies	antibody	NOUN
elements-9604	216	8	to	to	ADP
elements-9604	216	9	antigens	antigen	NOUN
elements-9604	216	10	;	;	PUNCT
elements-9604	216	11	however	however	ADV
elements-9604	216	12	,	,	PUNCT
elements-9604	216	13	elisa	elisa	PROPN
elements-9604	216	14	does	do	AUX
elements-9604	216	15	not	not	PART
elements-9604	216	16	reveal	reveal	VERB
elements-9604	216	17	the	the	DET
elements-9604	216	18	specific	specific	ADJ
elements-9604	216	19	location	location	NOUN
elements-9604	216	20	on	on	ADP
elements-9604	216	21	the	the	DET
elements-9604	216	22	antigen	antigen	NOUN
elements-9604	216	23	to	to	PART
elements-9604	216	24	which	which	PRON
elements-9604	216	25	the	the	DET
elements-9604	216	26	antibody	antibody	NOUN
elements-9604	216	27	binds	bind	VERB
elements-9604	216	28	.	.	PUNCT
elements-9604	217	1	further	further	ADJ
elements-9604	217	2	tests	test	NOUN
elements-9604	217	3	were	be	AUX
elements-9604	217	4	required	require	VERB
elements-9604	217	5	to	to	PART
elements-9604	217	6	determine	determine	VERB
elements-9604	217	7	the	the	DET
elements-9604	217	8	location	location	NOUN
elements-9604	217	9	of	of	ADP
elements-9604	217	10	where	where	SCONJ
elements-9604	217	11	the	the	DET
elements-9604	217	12	binding	binding	NOUN
elements-9604	217	13	of	of	ADP
elements-9604	217	14	ll25x	ll25x	PROPN
elements-9604	217	15	occurred	occur	VERB
elements-9604	217	16	on	on	ADP
elements-9604	217	17	gp140	gp140	PROPN
elements-9604	217	18	.	.	PUNCT
elements-9604	217	19	coomassie	coomassie	PROPN
elements-9604	217	20	blue	blue	ADJ
elements-9604	217	21	staining	stain	VERB
elements-9604	217	22	was	be	AUX
elements-9604	217	23	performed	perform	VERB
elements-9604	217	24	to	to	PART
elements-9604	217	25	determine	determine	VERB
elements-9604	217	26	the	the	DET
elements-9604	217	27	presence	presence	NOUN
elements-9604	217	28	and	and	CCONJ
elements-9604	217	29	quantity	quantity	NOUN
elements-9604	217	30	of	of	ADP
elements-9604	217	31	proteins	protein	NOUN
elements-9604	217	32	since	since	SCONJ
elements-9604	217	33	the	the	DET
elements-9604	217	34	dyes	dye	NOUN
elements-9604	217	35	bind	bind	VERB
elements-9604	217	36	directly	directly	ADV
elements-9604	217	37	to	to	ADP
elements-9604	217	38	the	the	DET
elements-9604	217	39	proteins	protein	NOUN
elements-9604	217	40	,	,	PUNCT
elements-9604	217	41	as	as	SCONJ
elements-9604	217	42	shown	show	VERB
elements-9604	217	43	in	in	ADP
elements-9604	217	44	figure	figure	NOUN
elements-9604	217	45	5b	5b	NUM
elements-9604	217	46	.	.	PUNCT
elements-9604	218	1	the	the	DET
elements-9604	218	2	coomassie	coomassie	PROPN
elements-9604	218	3	gel	gel	NOUN
elements-9604	218	4	displayed	display	VERB
elements-9604	218	5	equal	equal	ADJ
elements-9604	218	6	loading	loading	NOUN
elements-9604	218	7	amounts	amount	NOUN
elements-9604	218	8	of	of	ADP
elements-9604	218	9	both	both	CCONJ
elements-9604	218	10	gp140	gp140	PROPN
elements-9604	218	11	and	and	CCONJ
elements-9604	218	12	gp120	gp120	PROPN
elements-9604	218	13	.	.	PUNCT
elements-9604	219	1	western	western	ADJ
elements-9604	219	2	blotting	blotting	NOUN
elements-9604	219	3	was	be	AUX
elements-9604	219	4	performed	perform	VERB
elements-9604	219	5	to	to	PART
elements-9604	219	6	determine	determine	VERB
elements-9604	219	7	the	the	DET
elements-9604	219	8	locations	location	NOUN
elements-9604	219	9	on	on	ADP
elements-9604	219	10	antigens	antigen	NOUN
elements-9604	219	11	where	where	SCONJ
elements-9604	219	12	the	the	DET
elements-9604	219	13	binding	binding	NOUN
elements-9604	219	14	of	of	ADP
elements-9604	219	15	antibodies	antibody	NOUN
elements-9604	219	16	occurred	occur	VERB
elements-9604	219	17	,	,	PUNCT
elements-9604	219	18	such	such	ADJ
elements-9604	219	19	as	as	ADP
elements-9604	219	20	the	the	DET
elements-9604	219	21	location	location	NOUN
elements-9604	219	22	where	where	SCONJ
elements-9604	219	23	the	the	DET
elements-9604	219	24	binding	binding	NOUN
elements-9604	219	25	of	of	ADP
elements-9604	219	26	ll25x	ll25x	PROPN
elements-9604	219	27	occurred	occur	VERB
elements-9604	219	28	on	on	ADP
elements-9604	219	29	gp140	gp140	PROPN
elements-9604	219	30	.	.	PUNCT
elements-9604	219	31	figure	figure	NOUN
elements-9604	219	32	5a	5a	NUM
elements-9604	219	33	displays	display	VERB
elements-9604	219	34	the	the	DET
elements-9604	219	35	immunoblotting	immunoblotting	NOUN
elements-9604	219	36	of	of	ADP
elements-9604	219	37	su24	su24	PROPN
elements-9604	219	38	,	,	PUNCT
elements-9604	219	39	su343	su343	PROPN
elements-9604	219	40	,	,	PUNCT
elements-9604	219	41	and	and	CCONJ
elements-9604	219	42	ll25x	ll25x	NOUN
elements-9604	219	43	with	with	ADP
elements-9604	219	44	anti	anti	ADJ
elements-9604	219	45	-	-	ADJ
elements-9604	219	46	igg	igg	ADJ
elements-9604	219	47	-	-	PUNCT
elements-9604	219	48	hrp	hrp	PROPN
elements-9604	219	49	.	.	PUNCT
elements-9604	220	1	the	the	DET
elements-9604	220	2	gel	gel	NOUN
elements-9604	220	3	confirmed	confirm	VERB
elements-9604	220	4	successful	successful	ADJ
elements-9604	220	5	purification	purification	NOUN
elements-9604	220	6	of	of	ADP
elements-9604	220	7	antibodies	antibody	NOUN
elements-9604	220	8	since	since	SCONJ
elements-9604	220	9	antiigg	antiigg	NOUN
elements-9604	220	10	-	-	PUNCT
elements-9604	220	11	hrp	hrp	PROPN
elements-9604	220	12	was	be	AUX
elements-9604	220	13	able	able	ADJ
elements-9604	220	14	to	to	PART
elements-9604	220	15	bind	bind	VERB
elements-9604	220	16	to	to	ADP
elements-9604	220	17	all	all	DET
elements-9604	220	18	3	3	NUM
elements-9604	220	19	antibodies	antibody	NOUN
elements-9604	220	20	.	.	PUNCT
elements-9604	221	1	figure	figure	NOUN
elements-9604	221	2	5b	5b	NUM
elements-9604	221	3	displays	display	VERB
elements-9604	221	4	the	the	DET
elements-9604	221	5	binding	bind	VERB
elements-9604	221	6	specificity	specificity	NOUN
elements-9604	221	7	of	of	ADP
elements-9604	221	8	su24	su24	PROPN
elements-9604	221	9	,	,	PUNCT
elements-9604	221	10	su343	su343	PROPN
elements-9604	221	11	,	,	PUNCT
elements-9604	221	12	and	and	CCONJ
elements-9604	221	13	ll25x	ll25x	PROPN
elements-9604	221	14	,	,	PUNCT
elements-9604	221	15	which	which	PRON
elements-9604	221	16	were	be	AUX
elements-9604	221	17	used	use	VERB
elements-9604	221	18	as	as	ADP
elements-9604	221	19	primary	primary	ADJ
elements-9604	221	20	antibodies	antibody	NOUN
elements-9604	221	21	,	,	PUNCT
elements-9604	221	22	and	and	CCONJ
elements-9604	221	23	anti	anti	ADJ
elements-9604	221	24	-	-	ADJ
elements-9604	221	25	human	human	ADJ
elements-9604	221	26	igg	igg	PROPN
elements-9604	221	27	-	-	PUNCT
elements-9604	221	28	hrp	hrp	PROPN
elements-9604	221	29	was	be	AUX
elements-9604	221	30	used	use	VERB
elements-9604	221	31	as	as	ADP
elements-9604	221	32	a	a	DET
elements-9604	221	33	secondary	secondary	ADJ
elements-9604	221	34	antibody	antibody	NOUN
elements-9604	221	35	.	.	PUNCT
elements-9604	222	1	the	the	DET
elements-9604	222	2	results	result	NOUN
elements-9604	222	3	of	of	ADP
elements-9604	222	4	the	the	DET
elements-9604	222	5	western	western	ADJ
elements-9604	222	6	blots	blot	NOUN
elements-9604	222	7	supports	support	VERB
elements-9604	222	8	the	the	DET
elements-9604	222	9	data	datum	NOUN
elements-9604	222	10	received	receive	VERB
elements-9604	222	11	from	from	ADP
elements-9604	222	12	elisa	elisa	NOUN
elements-9604	222	13	.	.	PUNCT
elements-9604	223	1	su24	su24	PROPN
elements-9604	223	2	and	and	CCONJ
elements-9604	223	3	su343	su343	PROPN
elements-9604	223	4	were	be	AUX
elements-9604	223	5	revealed	reveal	VERB
elements-9604	223	6	to	to	PART
elements-9604	223	7	be	be	AUX
elements-9604	223	8	gp120specific	gp120specific	PROPN
elements-9604	223	9	,	,	PUNCT
elements-9604	223	10	and	and	CCONJ
elements-9604	223	11	thus	thus	ADV
elements-9604	223	12	,	,	PUNCT
elements-9604	223	13	bound	bind	VERB
elements-9604	223	14	to	to	ADP
elements-9604	223	15	both	both	DET
elements-9604	223	16	gp120	gp120	PROPN
elements-9604	223	17	and	and	CCONJ
elements-9604	223	18	gp140	gp140	PROPN
elements-9604	223	19	,	,	PUNCT
elements-9604	223	20	as	as	SCONJ
elements-9604	223	21	illustrated	illustrate	VERB
elements-9604	223	22	by	by	ADP
elements-9604	223	23	the	the	DET
elements-9604	223	24	dark	dark	PROPN
elements-9604	223	25	bands	band	NOUN
elements-9604	223	26	.	.	PUNCT
elements-9604	224	1	ll25x	ll25x	PROPN
elements-9604	224	2	was	be	AUX
elements-9604	224	3	revealed	reveal	VERB
elements-9604	224	4	to	to	PART
elements-9604	224	5	be	be	AUX
elements-9604	224	6	gp41	gp41	PROPN
elements-9604	224	7	-	-	PUNCT
elements-9604	224	8	specific	specific	ADJ
elements-9604	224	9	,	,	PUNCT
elements-9604	224	10	and	and	CCONJ
elements-9604	224	11	thus	thus	ADV
elements-9604	224	12	,	,	PUNCT
elements-9604	224	13	bound	bind	VERB
elements-9604	224	14	to	to	ADP
elements-9604	224	15	both	both	CCONJ
elements-9604	224	16	gp41	gp41	PROPN
elements-9604	224	17	and	and	CCONJ
elements-9604	224	18	gp140	gp140	PROPN
elements-9604	224	19	,	,	PUNCT
elements-9604	224	20	as	as	SCONJ
elements-9604	224	21	illustrated	illustrate	VERB
elements-9604	224	22	by	by	ADP
elements-9604	224	23	the	the	DET
elements-9604	224	24	dark	dark	PROPN
elements-9604	224	25	bands	band	NOUN
elements-9604	224	26	.	.	PUNCT
elements-9604	225	1	a	a	DET
elements-9604	225	2	band	band	NOUN
elements-9604	225	3	is	be	AUX
elements-9604	225	4	present	present	ADJ
elements-9604	225	5	at	at	ADP
elements-9604	225	6	the	the	DET
elements-9604	225	7	gp140	gp140	PROPN
elements-9604	225	8	location	location	NOUN
elements-9604	225	9	because	because	SCONJ
elements-9604	225	10	not	not	PART
elements-9604	225	11	all	all	PRON
elements-9604	225	12	of	of	ADP
elements-9604	225	13	the	the	DET
elements-9604	225	14	gp140	gp140	PROPN
elements-9604	225	15	was	be	AUX
elements-9604	225	16	cleaved	cleaved	ADJ
elements-9604	225	17	.	.	PUNCT
elements-9604	226	1	ll25x	ll25x	PROPN
elements-9604	226	2	binds	bind	VERB
elements-9604	226	3	to	to	PART
elements-9604	226	4	ectodomain	ectodomain	VERB
elements-9604	226	5	gp41	gp41	PROPN
elements-9604	226	6	that	that	SCONJ
elements-9604	226	7	that	that	PRON
elements-9604	226	8	remained	remain	VERB
elements-9604	226	9	a	a	DET
elements-9604	226	10	part	part	NOUN
elements-9604	226	11	of	of	ADP
elements-9604	226	12	env	env	NOUN
elements-9604	226	13	glycoprotein	glycoprotein	NOUN
elements-9604	226	14	gp140	gp140	PROPN
elements-9604	226	15	.	.	PUNCT
elements-9604	227	1	the	the	DET
elements-9604	227	2	lack	lack	NOUN
elements-9604	227	3	of	of	ADP
elements-9604	227	4	a	a	DET
elements-9604	227	5	band	band	NOUN
elements-9604	227	6	at	at	ADP
elements-9604	227	7	the	the	DET
elements-9604	227	8	gp120	gp120	NOUN
elements-9604	227	9	location	location	NOUN
elements-9604	227	10	indicates	indicate	VERB
elements-9604	227	11	that	that	SCONJ
elements-9604	227	12	ll25x	ll25x	PROPN
elements-9604	227	13	did	do	AUX
elements-9604	227	14	not	not	PART
elements-9604	227	15	bind	bind	VERB
elements-9604	227	16	to	to	ADP
elements-9604	227	17	the	the	DET
elements-9604	227	18	monomeric	monomeric	ADJ
elements-9604	227	19	surface	surface	NOUN
elements-9604	227	20	subunit	subunit	NOUN
elements-9604	227	21	gp120	gp120	NOUN
elements-9604	227	22	of	of	ADP
elements-9604	227	23	the	the	DET
elements-9604	227	24	env	env	NOUN
elements-9604	227	25	glycoprotein	glycoprotein	NOUN
elements-9604	227	26	gp140	gp140	PROPN
elements-9604	227	27	.	.	PUNCT
elements-9604	228	1	the	the	DET
elements-9604	228	2	band	band	NOUN
elements-9604	228	3	at	at	ADP
elements-9604	228	4	the	the	DET
elements-9604	228	5	gp41	gp41	PROPN
elements-9604	228	6	location	location	NOUN
elements-9604	228	7	indicates	indicate	VERB
elements-9604	228	8	that	that	SCONJ
elements-9604	228	9	ll25x	ll25x	PROPN
elements-9604	228	10	binds	bind	VERB
elements-9604	228	11	to	to	ADP
elements-9604	228	12	the	the	DET
elements-9604	228	13	ectodomain	ectodomain	NOUN
elements-9604	228	14	gp41	gp41	PROPN
elements-9604	228	15	of	of	ADP
elements-9604	228	16	env	env	PROPN
elements-9604	228	17	glycoprotein	glycoprotein	NOUN
elements-9604	228	18	gp140	gp140	PROPN
elements-9604	228	19	.	.	PUNCT
elements-9604	229	1	conclusion	conclusion	NOUN
elements-9604	229	2	the	the	DET
elements-9604	229	3	ll25x	ll25x	PROPN
elements-9604	229	4	dna	dna	PROPN
elements-9604	229	5	fragment	fragment	PROPN
elements-9604	229	6	was	be	AUX
elements-9604	229	7	successfully	successfully	ADV
elements-9604	229	8	cloned	clone	VERB
elements-9604	229	9	into	into	ADP
elements-9604	229	10	the	the	DET
elements-9604	229	11	mammalian	mammalian	ADJ
elements-9604	229	12	expression	expression	NOUN
elements-9604	229	13	vector	vector	NOUN
elements-9604	229	14	pcep4	pcep4	NOUN
elements-9604	229	15	by	by	ADP
elements-9604	229	16	digesting	digest	VERB
elements-9604	229	17	the	the	DET
elements-9604	229	18	ll25x	ll25x	ADJ
elements-9604	229	19	pcr	pcr	NOUN
elements-9604	229	20	products	product	NOUN
elements-9604	229	21	and	and	CCONJ
elements-9604	229	22	the	the	DET
elements-9604	229	23	cloning	cloning	NOUN
elements-9604	229	24	vector	vector	NOUN
elements-9604	229	25	pcep4	pcep4	NOUN
elements-9604	229	26	with	with	ADP
elements-9604	229	27	kpni	kpni	NOUN
elements-9604	229	28	and	and	CCONJ
elements-9604	229	29	sapi	sapi	NOUN
elements-9604	229	30	,	,	PUNCT
elements-9604	229	31	ligating	ligate	VERB
elements-9604	229	32	the	the	DET
elements-9604	229	33	resulting	result	VERB
elements-9604	229	34	ll25x	ll25x	NOUN
elements-9604	229	35	insert	insert	ADJ
elements-9604	229	36	vector	vector	NOUN
elements-9604	229	37	and	and	CCONJ
elements-9604	229	38	the	the	DET
elements-9604	229	39	resulting	result	VERB
elements-9604	229	40	pcep4	pcep4	ADJ
elements-9604	229	41	backbone	backbone	NOUN
elements-9604	229	42	vector	vector	NOUN
elements-9604	229	43	,	,	PUNCT
elements-9604	229	44	and	and	CCONJ
elements-9604	229	45	transforming	transform	VERB
elements-9604	229	46	e.	e.	PROPN
elements-9604	229	47	coli	coli	PROPN
elements-9604	229	48	top10f	top10f	NOUN
elements-9604	229	49	’	'	PUNCT
elements-9604	229	50	cells	cell	NOUN
elements-9604	229	51	with	with	ADP
elements-9604	229	52	the	the	DET
elements-9604	229	53	ligated	ligated	ADJ
elements-9604	229	54	product	product	NOUN
elements-9604	229	55	.	.	PUNCT
elements-9604	230	1	the	the	DET
elements-9604	230	2	ll25x	ll25x	PROPN
elements-9604	230	3	scfv	scfv	PROPN
elements-9604	230	4	fragment	fragment	NOUN
elements-9604	230	5	that	that	PRON
elements-9604	230	6	was	be	AUX
elements-9604	230	7	inserted	insert	VERB
elements-9604	230	8	and	and	CCONJ
elements-9604	230	9	fused	fuse	VERB
elements-9604	230	10	to	to	ADP
elements-9604	230	11	the	the	DET
elements-9604	230	12	fc	fc	PROPN
elements-9604	230	13	region	region	PROPN
elements-9604	230	14	(	(	PUNCT
elements-9604	230	15	ch2	ch2	PROPN
elements-9604	230	16	+	+	CCONJ
elements-9604	230	17	ch3	ch3	PROPN
elements-9604	230	18	)	)	PUNCT
elements-9604	230	19	of	of	ADP
elements-9604	230	20	human	human	ADJ
elements-9604	230	21	igg1	igg1	NOUN
elements-9604	230	22	in	in	ADP
elements-9604	230	23	the	the	DET
elements-9604	230	24	mammalian	mammalian	ADJ
elements-9604	230	25	expression	expression	NOUN
elements-9604	230	26	vector	vector	NOUN
elements-9604	230	27	pcep4	pcep4	NOUN
elements-9604	230	28	was	be	AUX
elements-9604	230	29	confirmed	confirm	VERB
elements-9604	230	30	and	and	CCONJ
elements-9604	230	31	verified	verify	VERB
elements-9604	230	32	by	by	ADP
elements-9604	230	33	dna	dna	PROPN
elements-9604	230	34	sequence	sequence	NOUN
elements-9604	230	35	analysis	analysis	NOUN
elements-9604	230	36	.	.	PUNCT
elements-9604	231	1	elisa	elisa	PROPN
elements-9604	231	2	determined	determine	VERB
elements-9604	231	3	that	that	SCONJ
elements-9604	231	4	the	the	DET
elements-9604	231	5	protein	protein	NOUN
elements-9604	231	6	,	,	PUNCT
elements-9604	231	7	ll25x	ll25x	PROPN
elements-9604	231	8	-	-	PUNCT
elements-9604	231	9	fc	fc	PROPN
elements-9604	231	10	,	,	PUNCT
elements-9604	231	11	binds	bind	VERB
elements-9604	231	12	to	to	PART
elements-9604	231	13	env	env	NOUN
elements-9604	231	14	glycoprotein	glycoprotein	NOUN
elements-9604	231	15	sivmac239gp140	sivmac239gp140	PROPN
elements-9604	231	16	and	and	CCONJ
elements-9604	231	17	not	not	PART
elements-9604	231	18	monomeric	monomeric	ADJ
elements-9604	231	19	surface	surface	NOUN
elements-9604	231	20	subunit	subunit	NOUN
elements-9604	231	21	sivmac239gp120	sivmac239gp120	PROPN
elements-9604	231	22	.	.	PUNCT
elements-9604	232	1	monoclonal	monoclonal	NOUN
elements-9604	232	2	sivmac239	sivmac239	PROPN
elements-9604	232	3	antibodies	antibodie	VERB
elements-9604	232	4	su24	su24	PROPN
elements-9604	232	5	and	and	CCONJ
elements-9604	232	6	su343	su343	PROPN
elements-9604	232	7	were	be	AUX
elements-9604	232	8	also	also	ADV
elements-9604	232	9	revealed	reveal	VERB
elements-9604	232	10	to	to	PART
elements-9604	232	11	bind	bind	VERB
elements-9604	232	12	to	to	ADP
elements-9604	232	13	monomeric	monomeric	ADJ
elements-9604	232	14	surface	surface	NOUN
elements-9604	232	15	subunit	subunit	NOUN
elements-9604	232	16	sivmac239gp120	sivmac239gp120	PROPN
elements-9604	232	17	and	and	CCONJ
elements-9604	232	18	env	env	PROPN
elements-9604	232	19	glycoprotein	glycoprotein	NOUN
elements-9604	232	20	sivmac“elisa	sivmac“elisa	PROPN
elements-9604	232	21	determined	determine	VERB
elements-9604	232	22	that	that	SCONJ
elements-9604	232	23	the	the	DET
elements-9604	232	24	protein	protein	NOUN
elements-9604	232	25	,	,	PUNCT
elements-9604	232	26	ll25x	ll25x	PROPN
elements-9604	232	27	-	-	PUNCT
elements-9604	232	28	fc	fc	PROPN
elements-9604	232	29	,	,	PUNCT
elements-9604	232	30	binds	bind	VERB
elements-9604	232	31	to	to	PART
elements-9604	232	32	env	env	NOUN
elements-9604	232	33	glycoprotein	glycoprotein	NOUN
elements-9604	232	34	sivmac239gp140	sivmac239gp140	PROPN
elements-9604	232	35	and	and	CCONJ
elements-9604	232	36	not	not	PART
elements-9604	232	37	monomeric	monomeric	ADJ
elements-9604	232	38	surface	surface	NOUN
elements-9604	232	39	subunit	subunit	NOUN
elements-9604	232	40	sivmac239gp120	sivmac239gp120	PROPN
elements-9604	232	41	.	.	PUNCT
elements-9604	232	42	”	"	PUNCT
elements-9604	233	1	78	78	NUM
elements-9604	233	2	elements	element	NOUN
elements-9604	233	3	:	:	PUNCT
elements-9604	233	4	:	:	PUNCT
elements-9604	233	5	spring	spring	NOUN
elements-9604	233	6	2017	2017	NUM
elements-9604	233	7	239gp140	239gp140	NUM
elements-9604	233	8	.	.	PUNCT
elements-9604	234	1	western	western	ADJ
elements-9604	234	2	blotting	blotting	NOUN
elements-9604	234	3	determined	determine	VERB
elements-9604	234	4	that	that	SCONJ
elements-9604	234	5	monoclonal	monoclonal	NOUN
elements-9604	234	6	sivmac239	sivmac239	PROPN
elements-9604	234	7	antibodies	antibodie	VERB
elements-9604	234	8	su24	su24	PROPN
elements-9604	234	9	and	and	CCONJ
elements-9604	234	10	su343	su343	PROPN
elements-9604	234	11	bind	bind	VERB
elements-9604	234	12	specifically	specifically	ADV
elements-9604	234	13	to	to	ADP
elements-9604	234	14	monomeric	monomeric	ADJ
elements-9604	234	15	surface	surface	NOUN
elements-9604	234	16	subunit	subunit	NOUN
elements-9604	234	17	sivmac239gp120	sivmac239gp120	NOUN
elements-9604	234	18	of	of	ADP
elements-9604	234	19	env	env	NOUN
elements-9604	234	20	glycoprotein	glycoprotein	NOUN
elements-9604	235	1	sivmac239gp140	sivmac239gp140	PROPN
elements-9604	235	2	.	.	PUNCT
elements-9604	236	1	western	western	ADJ
elements-9604	236	2	blotting	blotting	NOUN
elements-9604	236	3	also	also	ADV
elements-9604	236	4	revealed	reveal	VERB
elements-9604	236	5	that	that	SCONJ
elements-9604	236	6	monoclonal	monoclonal	NOUN
elements-9604	236	7	sivmac239	sivmac239	PROPN
elements-9604	236	8	antibody	antibody	NOUN
elements-9604	236	9	ll25x	ll25x	NOUN
elements-9604	236	10	binds	bind	VERB
elements-9604	236	11	specifically	specifically	ADV
elements-9604	236	12	to	to	PART
elements-9604	236	13	ectodomain	ectodomain	VERB
elements-9604	236	14	sivmac239gp41	sivmac239gp41	NUM
elements-9604	236	15	of	of	ADP
elements-9604	236	16	env	env	NOUN
elements-9604	236	17	glycoprotein	glycoprotein	NOUN
elements-9604	236	18	sivmac239gp140	sivmac239gp140	PROPN
elements-9604	236	19	.	.	PUNCT
elements-9604	237	1	future	future	ADJ
elements-9604	237	2	work	work	NOUN
elements-9604	237	3	should	should	AUX
elements-9604	237	4	be	be	AUX
elements-9604	237	5	directed	direct	VERB
elements-9604	237	6	at	at	ADP
elements-9604	237	7	testing	test	VERB
elements-9604	237	8	the	the	DET
elements-9604	237	9	ability	ability	NOUN
elements-9604	237	10	of	of	ADP
elements-9604	237	11	su24	su24	PROPN
elements-9604	237	12	,	,	PUNCT
elements-9604	237	13	su343	su343	PROPN
elements-9604	237	14	,	,	PUNCT
elements-9604	237	15	and	and	CCONJ
elements-9604	237	16	ll25x	ll25x	VERB
elements-9604	237	17	to	to	PART
elements-9604	237	18	neutralize	neutralize	VERB
elements-9604	237	19	diverse	diverse	ADJ
elements-9604	237	20	siv	siv	PROPN
elements-9604	237	21	strains	strain	NOUN
elements-9604	237	22	,	,	PUNCT
elements-9604	237	23	such	such	ADJ
elements-9604	237	24	as	as	ADP
elements-9604	237	25	the	the	DET
elements-9604	237	26	sensitive	sensitive	ADJ
elements-9604	237	27	strain	strain	NOUN
elements-9604	237	28	sivmac316	sivmac316	PROPN
elements-9604	237	29	and	and	CCONJ
elements-9604	237	30	the	the	DET
elements-9604	237	31	resistant	resistant	ADJ
elements-9604	237	32	strain	strain	NOUN
elements-9604	237	33	sivmac239	sivmac239	PROPN
elements-9604	237	34	.	.	PUNCT
elements-9604	238	1	acknowledgments	acknowledgments	PROPN
elements-9604	238	2	ismael	ismael	PROPN
elements-9604	238	3	fofana	fofana	PROPN
elements-9604	238	4	,	,	PUNCT
elements-9604	238	5	anindita	anindita	PROPN
elements-9604	238	6	sinha	sinha	PROPN
elements-9604	238	7	,	,	PUNCT
elements-9604	238	8	zachary	zachary	PROPN
elements-9604	238	9	strongin	strongin	NOUN
elements-9604	238	10	endnotes	endnote	NOUN
elements-9604	238	11	1	1	NUM
elements-9604	238	12	.	.	PUNCT
elements-9604	239	1	yates	yates	PROPN
elements-9604	239	2	,	,	PUNCT
elements-9604	239	3	nicole	nicole	PROPN
elements-9604	239	4	l.	l.	PROPN
elements-9604	239	5	et	et	PROPN
elements-9604	239	6	al	al	PROPN
elements-9604	239	7	.	.	PUNCT
elements-9604	240	1	“	"	PUNCT
elements-9604	240	2	vaccine	vaccine	NOUN
elements-9604	240	3	-	-	PUNCT
elements-9604	240	4	induced	induce	VERB
elements-9604	240	5	env	env	NOUN
elements-9604	240	6	v1	v1	NOUN
elements-9604	240	7	–	–	PUNCT
elements-9604	240	8	v2	v2	PROPN
elements-9604	240	9	igg3	igg3	NOUN
elements-9604	240	10	correlates	correlate	VERB
elements-9604	240	11	with	with	ADP
elements-9604	240	12	lower	low	ADJ
elements-9604	240	13	hiv-1	hiv-1	ADJ
elements-9604	240	14	infection	infection	NOUN
elements-9604	240	15	risk	risk	NOUN
elements-9604	240	16	and	and	CCONJ
elements-9604	240	17	declines	decline	VERB
elements-9604	240	18	soon	soon	ADV
elements-9604	240	19	after	after	ADP
elements-9604	240	20	vaccination	vaccination	NOUN
elements-9604	240	21	.	.	PUNCT
elements-9604	240	22	”	"	PUNCT
elements-9604	241	1	science	science	NOUN
elements-9604	241	2	translational	translational	ADJ
elements-9604	241	3	medicine	medicine	NOUN
elements-9604	241	4	6.228	6.228	NUM
elements-9604	241	5	(	(	PUNCT
elements-9604	241	6	2014	2014	NUM
elements-9604	241	7	):	):	PUNCT
elements-9604	241	8	228ra39	228ra39	NUM
elements-9604	241	9	.	.	PUNCT
elements-9604	241	10	pmc	pmc	PROPN
elements-9604	241	11	.	.	PROPN
elements-9604	242	1	2	2	NUM
elements-9604	242	2	.	.	X
elements-9604	242	3	ita	ita	PROPN
elements-9604	242	4	,	,	PUNCT
elements-9604	242	5	sergio	sergio	PROPN
elements-9604	242	6	et	et	PROPN
elements-9604	242	7	al	al	PROPN
elements-9604	242	8	.	.	PUNCT
elements-9604	243	1	“	"	PUNCT
elements-9604	243	2	analysis	analysis	NOUN
elements-9604	243	3	of	of	ADP
elements-9604	243	4	sivmac	sivmac	ADJ
elements-9604	243	5	-	-	PUNCT
elements-9604	243	6	specific	specific	ADJ
elements-9604	243	7	antibodies	antibody	NOUN
elements-9604	243	8	selected	select	VERB
elements-9604	243	9	via	via	ADP
elements-9604	243	10	phage	phage	NOUN
elements-9604	243	11	display	display	NOUN
elements-9604	243	12	”	"	PUNCT
elements-9604	243	13	aids	aids	PROPN
elements-9604	243	14	research	research	NOUN
elements-9604	243	15	and	and	CCONJ
elements-9604	243	16	human	human	ADJ
elements-9604	243	17	retroviruses	retrovirus	NOUN
elements-9604	243	18	(	(	PUNCT
elements-9604	243	19	2017	2017	NUM
elements-9604	243	20	)	)	PUNCT
elements-9604	243	21	.	.	PUNCT
elements-9604	244	1	references	reference	NOUN
elements-9604	244	2	yates	yates	PROPN
elements-9604	244	3	,	,	PUNCT
elements-9604	244	4	nicole	nicole	PROPN
elements-9604	244	5	l.	l.	PROPN
elements-9604	244	6	et	et	PROPN
elements-9604	244	7	al	al	PROPN
elements-9604	244	8	.	.	PUNCT
elements-9604	245	1	“	"	PUNCT
elements-9604	245	2	vaccine	vaccine	NOUN
elements-9604	245	3	-	-	PUNCT
elements-9604	245	4	induced	induce	VERB
elements-9604	245	5	env	env	NOUN
elements-9604	245	6	v1	v1	NOUN
elements-9604	245	7	–	–	PUNCT
elements-9604	245	8	v2	v2	PROPN
elements-9604	245	9	igg3	igg3	NOUN
elements-9604	245	10	correlates	correlate	VERB
elements-9604	245	11	with	with	ADP
elements-9604	245	12	lower	low	ADJ
elements-9604	245	13	hiv-1	hiv-1	ADJ
elements-9604	245	14	infection	infection	NOUN
elements-9604	245	15	risk	risk	NOUN
elements-9604	245	16	and	and	CCONJ
elements-9604	245	17	declines	decline	VERB
elements-9604	245	18	soon	soon	ADV
elements-9604	245	19	after	after	ADP
elements-9604	245	20	vaccination	vaccination	NOUN
elements-9604	245	21	.	.	PUNCT
elements-9604	245	22	”	"	PUNCT
elements-9604	246	1	science	science	NOUN
elements-9604	246	2	translational	translational	ADJ
elements-9604	246	3	medicine	medicine	NOUN
elements-9604	246	4	6.228	6.228	NUM
elements-9604	246	5	(	(	PUNCT
elements-9604	246	6	2014	2014	NUM
elements-9604	246	7	):	):	PUNCT
elements-9604	246	8	228ra39	228ra39	NUM
elements-9604	246	9	.	.	PUNCT
elements-9604	247	1	pmc	pmc	PROPN
elements-9604	247	2	.	.	PROPN
elements-9604	247	3	web	web	PROPN
elements-9604	247	4	.	.	PROPN
elements-9604	247	5	6	6	NUM
elements-9604	247	6	may	may	PROPN
elements-9604	247	7	2016	2016	NUM
elements-9604	247	8	.	.	PUNCT
elements-9604	248	1	ita	ita	PROPN
elements-9604	248	2	,	,	PUNCT
elements-9604	248	3	sergio	sergio	PROPN
elements-9604	248	4	et	et	PROPN
elements-9604	248	5	al	al	PROPN
elements-9604	248	6	.	.	PUNCT
elements-9604	249	1	“	"	PUNCT
elements-9604	249	2	analysis	analysis	NOUN
elements-9604	249	3	of	of	ADP
elements-9604	249	4	sivmac	sivmac	ADJ
elements-9604	249	5	-	-	PUNCT
elements-9604	249	6	specific	specific	ADJ
elements-9604	249	7	antibodies	antibody	NOUN
elements-9604	249	8	selected	select	VERB
elements-9604	249	9	via	via	ADP
elements-9604	249	10	phage	phage	NOUN
elements-9604	249	11	display	display	NOUN
elements-9604	249	12	”	"	PUNCT
elements-9604	249	13	aids	aids	PROPN
elements-9604	249	14	research	research	NOUN
elements-9604	249	15	and	and	CCONJ
elements-9604	249	16	human	human	ADJ
elements-9604	249	17	retroviruses	retrovirus	NOUN
elements-9604	249	18	(	(	PUNCT
elements-9604	249	19	2017	2017	NUM
elements-9604	249	20	)	)	PUNCT
elements-9604	249	21	.	.	PUNCT
elements-9604	250	1	accepted	accept	VERB
elements-9604	250	2	.	.	PUNCT
elements-9604	251	1	79	79	NUM
elements-9604	251	2	the	the	DET
elements-9604	251	3	road	road	NOUN
elements-9604	251	4	to	to	ADP
elements-9604	251	5	a	a	DET
elements-9604	251	6	cure	cure	NOUN