Microsoft Word - ETASR_V11_N5_pp7558-7563 Engineering, Technology & Applied Science Research Vol. 11, No. 5, 2021, 7558-7563 7558 www.etasr.com Shar et al.: Health Risk Assessment of Arsenic in the Drinking Water of Upper Sindh, Pakistan Health Risk Assessment of Arsenic in the Drinking Water of Upper Sindh, Pakistan Noor Ul Hassan Shar Institute of Chemistry Shah Abdul Latif University Khairpur, Pakistan noorulhassan4228@gmail.com Ghulam Qadir Shar Institute of Chemistry Shah Abdul Latif University Khairpur, Pakistan gqadir.shar@salu.edu.pk Abdul Raheem Shar Institute of Chemistry Shah Abdul Latif University Khairpur, Pakistan saifiraheem3863920@gmail.com Shafi Muhammad Wassan Department of Community Medicine Ghulam Muhammad Mahar Medical College Sukkur Sindh, Pakistan wassanshafi1975@gmail.com Zubeda Bhatti Department of Physics Shah Abdul Latif University Khairpur, Pakistan bhatti.zubeda@yahoo.com Abida Ali Department of Computer Science Shah Abdul Latif University Khairpur, Pakistan abida.ali@mckru.edu.pk Abstract-Water is a valuable compound for plants, animals, and humans. Various contaminating agents pollute it, with arsenic being one of them. Measurements of arsenic in potable water in Upper Sindh were conducted during this study. The samples were prepared by microwave-assisted digestion and analyzed by an atomic absorption spectrophotometer. A total of 240 potable water samples were collected from 8 Talukas of Upper Sindh. DMS coordinates were also recorded with the help of the Global Positioning System (GPS). The highest arsenic content of 50µg/L was observed in Garhi Khairo Taluka. The average arsenic content in water samples of all of the Talukas, except Miro Khan, was found higher than the WHO permissible limit. The 69.2% of samples were found to be contaminated by arsenic. Therefore, the water of the studied area is concluded to be in poor condition for cooking and drinking. Keywords-arsenic; upper Sindh; atomic absorption spectrophotometer; water health risk assessment I. INTRODUCTION Arsenic is a metalloid with high toxicity, which can be found in the environment in geological substrata, whereas it can also be released by human activities [1]. In numerous areas, arsenic in freshwater atmosphere, due to direct release from anthropogenic and natural sources, is a severe concern [2]. The concentration of arsenic ranges from 0.5 to 5000µg/L in freshwater [3]. Four oxidation states of arsenic are observed in nature: (-3), (0), (+3) and (+5) whereas it can be found in various forms such as arsenate, arsenite, monomethylarsonic acid, dimethyl arsenic acid, trimethylarsine, arsenocholine, arsenobetaine, arsenosugras, etc. Organic arsenic is less poisonous than inorganic arsenic, while pentavalent arsenic is less toxic than its trivalent state [4]. Arsenic is accumulated in soil through human activities like fuel utilization, mining, smelting of arsenic ores, manufacture of arsenic–based compounds, and use of arsenic–based pesticides [5, 6]. Biomethylation mechanism of micro-organisms and use of organo-arsenical pesticides are responsible for organic arsenic in natural water systems [7]. Higher concentrations of arsenic in groundwater of countries like Italy, Vietnam, Bangladesh, West Bengal, Hungary, China, Mexico, Chile, and Argentina have been observed [8]. Water resources are exploited because of the continuous development of global population. Arsenic and heavy metal contamination of water sources are often found to be in extremely critical state [9]. These pollutants find their ways in surface and groundwater sources due to their high mobility. There are various groundwater and surface water sources [10]. When compared to surface water, groundwater is more secure regarding heavy metals and microbes [11]. Therefore, most people use groundwater for drinking purposes [12], although a lot of research has been conducted on groundwater containing heavy metals [13]. About 100 million people of Southeast Asia were found to be at high risk of arsenic toxicity [14]. There are two main ways of arsenic accumulation in the human body, taking arsenic polluted potable water and consuming arsenic contaminated food [15]. Stomach toxicity, brain, kidney, and liver cancers, and skin lesions may be the results of chronic Corresponding author: Abdul Raheem Shar Engineering, Technology & Applied Science Research Vol. 11, No. 5, 2021, 7558-7563 7559 www.etasr.com Shar et al.: Health Risk Assessment of Arsenic in the Drinking Water of Upper Sindh, Pakistan effects of arsenic. Due to its toxicity, WHO and USEPA have reduced the threshold limit of arsenic from 50 to 10µg/L in 2001 [16]. To the best of our knowledge, in the 8 Talukas of upper Sindh that constitute the studied area, arsenic contamination and health risk assessment in potable water have not been conducted. The present work aimed to identify arsenic contamination and its potential health risk assessment in upper Sindh keeping in view the public, anthropogenic inputs and geology. II. MATERIALS AND METHODS A. Study Area The weather of upper Sindh is moderate in winter and extremely hot in summer. The area is commonly considered as a hot arid zone. The lowest and the highest temperatures recorded are -3.9ºC and 52.8 0 C respectively. In the season of monsoon from July to September the rainfall is not sufficient. The average recorded annual rainfall is 122.5mm and the air is generally dry. Upper Sindh lies from 27°56' to 28°27' N and from 68° to 69°44' E covering an area of about 6,790km 2 . In its surroundings, Thar Desert is located on the eastern side, Bahurai range in west, Kherthar range in south west and Suleman range in the north. At the south eastern side of Jacobabad, Indus River flows from north to south and besides the Bolan River there are various canals and streams at the western and northern sides. There are parts of Baluchistan highlands at northern, western, and south western areas and as a result, non-perennial streams are found towards the province of Sindh [17]. Fig. 1. Hand pumps in the study area. B. Chemicals and Reagents Reagents bought from Merck (Darmstadt, Germany) along with de-ionized water were used for arsenic solution preparation. C. Sampling In order to get groundwater samples it is necessary to run hand pumps at least for 5 minutes to eliminate insoluble impurities and sand particles and to get depth water of required elemental amounts [18]. Water used for drinking was obtained from hand pumps whose depth was varied from 30 to 70 feet. Plastic 1500 mL bottles were used to collect the water samples. Eight Talukas of upper Sindh were selected to be sampled. The coordinates were recorded with the help of a GPS device. In total, 240 potable water samples were collected from 8 Talukas of upper Sindh. After collection, the water samples were transported immediately to the laboratory. Practical investigation of arsenic was conducted in the laboratory and its quantity was measured with the help of a calibration graph. D. Instrumentation To measure the arsenic contamination from potable water, the technique of Atomic Absorption Spectrophotometer (Perkin-A 700) was used along with Mercury Hydride System (MHS–15). It is a precise and simple method of analysis of metals found in various samples [19]. E. Microwave Digestion Method PTFE flasks were taken and 500mL of water samples were kept in them. After closing tightly, the flasks were subjected to microwave radiation in stopped up vessel microwave digestion using Milestone Ethos D model (Sorisole-Bg, Italy). The digestion plan of microwave oven was 100W for 2min, 250W for 6min, 400W for 5min, 550W for 8min, and ventilation for 8min. After the cooling, the content of the flask was diluted with 0.2M nitric acid to 10mL. The same procedure was followed to prepare the reagent blank. The advantage of microwave digestion method is that it takes less time to digest water samples and the possibility of evaporation of elements is less, therefore more precise extraction of elements from samples can be accomplished as compared to conventional digestion methods. Less acid is used for digestion as well [20]. III. RESULTS AND DISCUSSION More than 40% of the population of Pakistan suffers from arsenic contamination in potable water. More than 20% of the people of Punjab suffer from arsenic contamination in surface or groundwater sources, with the problem becoming bigger in industrial zones [21-24]. According to our results, the highest concentration of 50µg/L of arsenic was obtained in Garhi Khairo Taluka and the minimum of 3µg/L was found in samples from Ubauro, Tangwani, Kambar, and Miro Khan Talukas. The mean arsenic content in all Talukas except Miro Khan was higher than the WHO permissible limit. In Daharki and Kambar Talukas, 80% of groundwater samples were contaminated by arsenic, in Kashmore and Garhi Khairo 86.7%, and in Ubauro, Tangwani, Thul, and Miro Khan the contamination percentage was 70%, 73%, 56.7%, and 20% respectively (Table I). Water quality is worse in heavily populated regions of Pakistan such as Peshawar, Lahore, Karachi, Shaheed Benazirabad [49] and other cities and towns. The groundwater of upper Sindh is also contaminated and not safe for drinking due to its high arsenic contamination. Some reported cities of Pakistan with high arsenic content in groundwater are shown in Table II. Arsenic concentration in various countries is given in Table III. Descriptive statistics such as, minimum, maximum, mean and standard deviation of arsenic in potable water of upper Sindh are given in Table IV. Engineering, Technology & Applied Science Research Vol. 11, No. 5, 2021, 7558-7563 7560 www.etasr.com Shar et al.: Health Risk Assessment of Arsenic in the Drinking Water of Upper Sindh, Pakistan TABLE I. ARSENIC CONCENTRATION (µg/L) IN THE STUDIED EIGHT TALUKAS OF UPPER SINDH Daharki Ubauro Kashmore Tangwani Garhi Khario Thul Kambar Miro Khan 5 10 9 21 17 38 11 9 11 12 12 7 16 13 3 6 14 20 12 9 12 12 16 4 11 18 13 18 11 8 21 5 5 19 10 19 4 7 12 6 10 26 14 16 9 8 3 5 11 18 11 12 20 5 15 18 14 15 10 20 13 16 21 17 15 12 12 33 12 9 18 16 5 24 15 24 12 8 26 17 16 37 12 37 4 8 34 7 17 25 16 10 17 6 49 5 13 38 18 8 22 13 26 3 15 25 18 6 13 18 11 6 5 3 13 9 4 9 10 7 19 14 15 14 16 8 16 9 20 13 19 16 22 12 15 7 17 11 9 11 19 18 11 9 14 17 14 17 27 15 10 6 13 9 13 25 35 23 3 15 18 6 13 39 50 31 8 6 17 5 14 25 27 46 19 7 10 7 15 3 12 23 12 15 13 9 17 9 11 8 11 7 11 18 24 22 16 7 15 10 12 19 27 20 13 12 12 5 14 18 17 19 34 9 33 6 13 20 17 26 25 30 24 7 13 5 21 18 20 21 19 5 27 6 12 15 30 16 29 9 TABLE II. CONCENTRATION OF ARSENIC IN DIFFERENT CITIES OF PAKISTAN Province Area As (µg/L) References Sindh Sajawal 15.3 [25] Ghorabari 50 [26] MirpurSakro 80 [26] Ketibandar 5–25 [27] Khairpur 0.24−315.6 [28] Gambat 0.01–126 [29] Shaheed Benazirabad 10–200 [30] Dadu 8–67 [31] Thatta 10–200 [32] Jamshoro 13−106 [33] Punjab Sheikhupura 5–76 [34] Rahimyar Khan 20–500 [35] Muzffargarh 0.01−900 [36] Dera Gazi Khan 1–29 [37] Balochistan Sibi 0.3–3.5 [38] TABLE III. ARSENIC CONCENTRATION IN VARIOUS COUNTRIES Country Concentration (µg/L) Reference India 44 [39] China (Shanxi) 1932 [40] Afghanistan 100 [41] Taiwan 1800 [42] Greece > 20 [43] Bangladesh 398 [44] Veitnam 3100 [45] Nepal 260 [46] Cambodia 3500 [47] Inner Mongolia, China 4000 [48] TABLE IV. DESCRIPTIVE STATISTICS OF ARSENIC IN POTABLE WATER OF UPPER SINDH Taluka N Min Max Mean Std. deviation Daharki 30 5 27 13.3 4.78 Ubauro 30 3 38 16.0 8.74 Kashmore 30 9 27 14.7 4.18 Tangwani 30 3 39 17.6 8.85 Garhi Khario 30 4 50 18.1 10.11 Thul 30 5 46 15.2 10.00 Kambar 30 3 49 17.1 10.11 Miro Khan 30 3 18 8.5 4.32 Engineering, Technology & Applied Science Research Vol. 11, No. 5, 2021, 7558-7563 7561 www.etasr.com Shar et al.: Health Risk Assessment of Arsenic in the Drinking Water of Upper Sindh, Pakistan A. Pearson Correlation Coefficient The Pearson correlation of the potable water of the 8 talukas of upper Sindh is shown in Table V. Positive correlation was observed among Garhi Khairo and Daharki (0.483). Arsenic in potable water of Thul and Garhi Khairo also showed positive correlation of 0.491, whereas the water of the Kambar and Ubauro Talukas also displayed positive correlation of 0.415. Negative correlation of arsenic in drinking water was found among Talukas Thul and Ubauro. All relations were observed significant at the level of 0.05, and 0.1 respectively. B. Human health risk assessment Equation (1) was used to estimate the total arsenic intake [33]: ADI = �� � �� (1) Body weight (BW) and daily water intake for common people were supposed to be 65kg and 3 to 3.5L respectively. The results of the Average Daily Intake (ADI) are given in Table VI. ADI values at alarming levels were found in the water of all Talukas of upper Sindh with the exception of Miro Khan. The values ranged from 0.46 to 0.97µg/day. Comparatively, Tangwani, Garhi Khairo, and Kambar Talukas showed higher ADI values. Various problems may be caused due to high ADI values such as diabetes, cardiovascular problems, hypertension, black foot disease, keratosis, bladder and lung cancer, and skin lesions [31] (Tables VI, VII). TABLE V. CORRELATION COEFFICIENT OF ARSENIC IN GROUNDWATER OF DIFFERENT UPPER SINDH TALUKAS Daharki Ubauro Kashmore Tangwani Garhi Khario Thul Kambar Miro Khan Daharki 1 Ubauro -0.097 1 Kashmore 0.057 0.150 1 Tangwani 0.097 0.023 -0.063 1 Garhi Khario 0.483 ** -0.308 0.040 0.319 1 Thul 0.095 -0.432 * -0.132 0.262 0.491 ** 1 Kambar 0.306 0.415 * 0.092 0.116 -0.004 -0.197 1 Miro Khan -0.136 -0.217 -0.300 0.159 -0.030 -0.029 -0.074 1 TABLE VI. AVERAGE DAILY INTAKE OF ARSENIC FROM DRINKING WATER OF DIFFERENT UPPER SINDH TALUKAS Daharki Ubauro Kashmore Tangwani Garhi Khario Thul Kambar Miro Khan 0.27 0.54 0.48 1.13 0.92 2.05 0.59 0.48 0.59 0.65 0.65 0.38 0.86 0.70 0.16 0.32 0.75 1.08 0.65 0.48 0.65 0.65 0.86 0.22 0.59 0.97 0.70 0.97 0.59 0.43 1.13 0.27 0.27 1.02 0.54 1.02 0.22 0.38 0.65 0.32 0.54 1.40 0.75 0.86 0.48 0.43 0.16 0.27 0.59 0.97 0.59 0.65 1.08 0.27 0.81 0.97 0.75 0.81 0.54 1.08 0.70 0.86 1.13 0.92 0.81 0.65 0.65 1.78 0.65 0.48 0.97 0.86 0.27 1.29 0.81 1.29 0.65 0.43 1.40 0.92 0.86 1.99 0.65 1.99 0.22 0.43 1.83 0.38 0.92 1.35 0.86 0.54 0.92 0.32 2.64 0.27 0.70 2.05 0.97 0.43 1.18 0.70 1.40 0.16 0.81 1.35 0.97 0.32 0.70 0.97 0.59 0.32 0.27 0.16 0.70 0.48 0.22 0.48 0.54 0.38 1.02 0.75 0.81 0.75 0.86 0.43 0.86 0.48 1.08 0.70 1.02 0.86 1.18 0.65 0.81 0.38 0.92 0.59 0.48 0.59 1.02 0.97 0.59 0.48 0.75 0.92 0.75 0.92 1.45 0.81 0.54 0.32 0.70 0.48 0.70 1.35 1.88 1.24 0.16 0.81 0.97 0.32 0.70 2.10 2.69 1.67 0.43 0.32 0.92 0.27 0.75 1.35 1.45 2.48 1.02 0.38 0.54 0.38 0.81 0.16 0.65 1.24 0.65 0.81 0.70 0.48 0.92 0.48 0.59 0.43 0.59 0.38 0.59 0.97 1.29 1.18 0.86 0.38 0.81 0.54 0.65 1.02 1.45 1.08 0.70 0.65 0.65 0.27 0.75 0.97 0.92 1.02 1.83 0.48 1.78 0.32 0.70 1.08 0.92 1.40 1.35 1.62 1.29 0.38 0.70 0.27 1.13 0.97 1.08 1.13 1.02 0.27 1.45 0.32 0.65 0.81 1.62 0.86 1.56 0.48 Note: Safe arsenic daily intake in water is 0.66µg/day Engineering, Technology & Applied Science Research Vol. 11, No. 5, 2021, 7558-7563 7562 www.etasr.com Shar et al.: Health Risk Assessment of Arsenic in the Drinking Water of Upper Sindh, Pakistan TABLE VII. MEAN ADI OF ARSENIC IN POTABLE WATER OF THE UPPER SINDH TALUKAS Taluka Mean (µg/L) ADI Mean (µg/day) Daharki 13.3 0.72 Ubauro 16 0.86 Kashmore 14.7 0.79 Tangwani 17.6 0.95 Garhi Khairo 18.1 0.97 Thul 15.2 0.82 Kambar 17.1 0.92 Miro Khan 8.5 0.46 IV. CONCLUSION From the results obtained from the present work, it can be concluded that the groundwater of upper Sindh is unfit for drinking due to the presence of arsenic in high amounts. With the exception of Miro Khan where only 20% of water samples were found to be contaminated, the potable water of the Talukas was found highly contaminated due to the existence of arsenic concentration at alarming levels. Contaminated water may cause various health hazards. Therefore, it is suggested to the government of Sindh to pay special consideration to the matter. It is also recommended that awareness among farmers must be increased to use chemicals, particularly pesticides, carefully. Also, the government should provide safe drinking water by installing Reverse Osmosis (RO) plants in areas where people are using unsafe water for drinking and cooking. REFERENCES [1] S. Sher and A. Rehman, "Use of heavy metals resistant bacteria—a strategy for arsenic bioremediation," Applied Microbiology and Biotechnology, vol. 103, no. 15, pp. 6007–6021, Aug. 2019, https://doi.org/10.1007/s00253-019-09933-6. [2] A. N. Chowdhury et al., "Arsenic in freshwater ecosystems of the Bengal delta: status, sources and seasonal variability," Toxicological & Environmental Chemistry, vol. 97, no. 5, pp. 538–551, May 2015, https://doi.org/10.1080/02772248.2015.1053482. [3] R. Foroutan et al., "Efficient arsenic(V) removal from contaminated water using natural clay and clay composite adsorbents," Environmental Science and Pollution Research, vol. 26, no. 29, pp. 29748–29762, Oct. 2019, https://doi.org/10.1007/s11356-019-06070-5. [4] J. Tapia, J. Murray, M. Ormachea, N. Tirado, and D. K. Nordstrom, "Origin, distribution, and geochemistry of arsenic in the Altiplano-Puna plateau of Argentina, Bolivia, Chile, and Perú," Science of The Total Environment, vol. 678, pp. 309–325, Aug. 2019, https://doi.org/10.1016/ j.scitotenv.2019.04.084. [5] X. Wan, M. Lei, and T. Chen, "Review on remediation technologies for arsenic-contaminated soil," Frontiers of Environmental Science & Engineering, vol. 14, no. 2, Dec. 2019, Art. no. 24, https://doi.org/ 10.1007/s11783-019-1203-7. [6] S. Rai, D. Singh, and A. K. Singh, in Mycoremediation of Arsenic from Contaminated Soil for Sustainable Agriculture, New Delhi, India: Todya & Tomorrow’s Printers and Publishers, 2020, pp. 575–591. [7] E. Shaji, M. Santosh, K. V. Sarath, P. Prakash, V. Deepchand, and B. V. Divya, "Arsenic contamination of groundwater: A global synopsis with focus on the Indian Peninsula," Geoscience Frontiers, vol. 12, no. 3, May 2021, Art. no. 101079, https://doi.org/10.1016/j.gsf.2020.08.015. [8] S. Bolisetty, M. Peydayesh, and R. Mezzenga, "Sustainable technologies for water purification from heavy metals: review and analysis," Chemical Society Reviews, vol. 48, no. 2, pp. 463–487, Jan. 2019, https://doi.org/ 10.1039/C8CS00493E. [9] K. Khan et al., "Prevalent fecal contamination in drinking water resources and potential health risks in Swat, Pakistan," Journal of Environmental Sciences, vol. 72, pp. 1–12, Oct. 2018, https://doi.org/ 10.1016/j.jes.2017.12.008. [10] S. O. Olasoji, N. O. Oyewole, B. Abiola, and J. N. Edokpayi, "Water Quality Assessment of Surface and Groundwater Sources Using a Water Quality Index Method: A Case Study of a Peri-Urban Town in Southwest, Nigeria," Environments, vol. 6, no. 2, Feb. 2019, Art. no. 23, https://doi.org/10.3390/environments6020023. [11] D. Wang, J. Wu, Y. Wang, and Y. Ji, "Finding High-Quality Groundwater Resources to Reduce the Hydatidosis Incidence in the Shiqu County of Sichuan Province, China: Analysis, Assessment, and Management," Exposure and Health, vol. 12, no. 2, pp. 307–322, Jun. 2020, https://doi.org/10.1007/s12403-019-00314-y. [12] H. Rajkumar, P. K. Naik, and M. S. Rishi, "A new indexing approach for evaluating heavy metal contamination in groundwater," Chemosphere, vol. 245, Apr. 2020, Art. no. 125598, https://doi.org/10.1016/ j.chemosphere.2019.125598. [13] M. Shahid, C. Dumat, N. Khan Niazi, S. Khalid, and Natasha, "Global scale arsenic pollution : increase the scientific knowledge to reduce human exposure," VertigO - la revue électronique en sciences de l’environnement, no. Hors-série 31, Sep. 2018, https://doi.org/ 10.4000/vertigo.21331. [14] M. Ozturk et al., "Arsenic and Human Health: Genotoxicity, Epigenomic Effects, and Cancer Signaling," Biological Trace Element Research, Apr. 2021, https://doi.org/10.1007/s12011-021-02719-w. [15] M. Costa, "Review of arsenic toxicity, speciation and polyadenylation of canonical histones," Toxicology and Applied Pharmacology, vol. 375, pp. 1–4, Jul. 2019, https://doi.org/10.1016/j.taap.2019.05.006. [16] D. Mohan and C. U. Pittman, "Arsenic removal from water/wastewater using adsorbents—A critical review," Journal of Hazardous Materials, vol. 142, no. 1, pp. 1–53, Apr. 2007, https://doi.org/10.1016/ j.jhazmat.2007.01.006. [17] S. Ali, "Heavy Downpour Event over upper Sindh in September, 2012," Pakistan Journal of Meteorology, vol. 9, no. 18, pp. 59–72, 2013. [18] A. R. Shar, G. Q. Shar, W. B. Jatoi, N.-U.-H. Shar, L. A. Shar, and W. M. Ghouri, "Assessment of the quality of drinking water of Thari Mirwah Town and surrounding villages, District Khairpur, Sindh, Pakistan," Pakistan Journal of Analytical & Environmental Chemistry, vol. 15, no. 2, pp. 39–59, 2014. [19] J. B. Fisher, K. P. Tu, and D. D. Baldocchi, "Global estimates of the land–atmosphere water flux based on monthly AVHRR and ISLSCP-II data, validated at 16 FLUXNET sites," Remote Sensing of Environment, vol. 112, no. 3, pp. 901–919, Mar. 2008, https://doi.org/10.1016/ j.rse.2007.06.025. [20] D. Guven and G. Akinci, "Comparison of acid digestion techniques to determine heavy metals in sediment and soil samples," Gazi University Journal of Science, vol. 24, no. 1, pp. 29–34, Jan. 2011. [21] I. S. Khalid and A. A. Khaver, Political Economy of Water Pollution in Pakistan: An Overview. Islamabad, Pakistan: Sustainable Development Policy Institute, 2019. [22] A. Kumar and C. K. Singh, "Arsenic enrichment in groundwater and associated health risk in Bari doab region of Indus basin, Punjab, India," Environmental Pollution, vol. 256, Jan. 2020, Art. no. 113324, https://doi.org/10.1016/j.envpol.2019.113324. [23] F. Rehman et al., "Groundwater quality and potential health risks caused by arsenic (As) in Bhakkar, Pakistan," Environmental Earth Sciences, vol. 79, no. 24, Nov. 2020, Art. no. 529, https://doi.org/10.1007/s12665- 020-09270-2. [24] A. Shahab, S. Qi, and M. Zaheer, "Arsenic contamination, subsequent water toxicity, and associated public health risks in the lower Indus plain, Sindh province, Pakistan," Environmental Science and Pollution Research, vol. 26, no. 30, pp. 30642–30662, Oct. 2019, https://doi.org/ 10.1007/s11356-018-2320-8. [25] A. Shar et al., "Quality Characteristics and Risk Assessment of Arsenic in Drinking Water of Different Villages of District Sujawal, Sindh, Pakistan," International Journal of Biosciences (IJB), vol. 17, no. 3, pp. 287–294, Jun. 2020. [26] A. R. Shar, G. Q. Shar, S. M. Wassan, Z. Bhatti, A. A. Shar, and N.-H. Shar, "Risk Assessment of Arsenic and Cadmium in Groundwater of Engineering, Technology & Applied Science Research Vol. 11, No. 5, 2021, 7558-7563 7563 www.etasr.com Shar et al.: Health Risk Assessment of Arsenic in the Drinking Water of Upper Sindh, Pakistan Talukas Ghorabari and Mirpur Sakro, Sindh, Pakistan," Pakistan Journal of Analytical & Environmental Chemistry, vol. 22, no. 1, pp. 100–114, Jun. 2021, https://doi.org/10.21743/pjaec/2021.06.11. [27] A. Shar et al., "Risk assessment of arsenic and heavy metals of drinking water in coastal area: Case for Taluka Keti Bandar, Sindh, Pakistan," Journal of Biodiversity and Environmental Sciences, vol. 16, no. 6, pp. 52–64, Aug. 2020. [28] M. A. Jakhrani, A. J. Chaudhray, K. M. Malik, M. Q. Mazari, A. A. Jakhrani, and M. ul-Hassan, "Determination of Arsenic and Other Heavy Metals in Hand Pump and Tube-Well Ground Water of Khairpur, Sindh, Pakistan," in Second International Conference on Environmental and Computer Science, Dubai, United Arab Emirates, Dec. 2009, pp. 271– 276, https://doi.org/10.1109/ICECS.2009.94. [29] M. A. Jakhrani, K. M. Malik, S. Sahito, and A. A. Jakhrani, "Analytical Investigation of Arsenic and Iron in hand pump and tube-well groundwater of Gambat, Sindh, Pakistan," Pakistan Journal Chemistry, vol. 1, no. 3, pp. 140–144, 2011. [30] A. J. Kandhro, N. A. Samoon, and J. H. Laghari, "Assessment of arsenic and essential metal ions in the quality of groundwater sources of Taluka Daur, District Shaheed Benazeer Abad, Sindh, Pakistan," Journal of Peoples University of Medical & Health Sciences, vol. 6, no. 1, pp. 1–8, 2016. [31] A. Memon, G. Lund, N. Channa, S. Shah, M. Younis, and F. Buriro, "Contaminants Exposure and Impacts on Drinking Water of Johi Subdivision of Sindh, Pakistan," Science Letters, vol. 4, no. 1, pp. 78–83, Jan. 2016. [32] G. Rubab, S. Naseem, A. Khan, V. Husain, and G. M. Arain, "Distribution and sources of arsenic contaminated groundwater in parts of Thatta district, Sindh," Journal of Himalayan Earth Sciences, vol. 47, no. 2, pp. 175–183, 2014. [33] J. A. Baig et al., "Evaluation of arsenic and other physico-chemical parameters of surface and ground water of Jamshoro, Pakistan," Journal of Hazardous Materials, vol. 166, no. 2, pp. 662–669, Jul. 2009, https://doi.org/10.1016/j.jhazmat.2008.11.069. [34] M. Abbas and K. J. Cheema, "Arsenic levels in drinking water and associated health risk in district Sheikhupura, Pakistan," The Journal of Animal & Plant Sciences, vol. 25, no. 3, pp. 719–724, 2015. [35] M. T. Mahar, M. Y. Khuhawar, T. M. Jahangir, and M. A. Baloch, "Determination of arsenic contents in groundwater of District Rahim Yar Khan Southern Punjab, Pakistan," Arabian Journal of Geosciences, vol. 8, no. 12, pp. 10983–10994, Dec. 2015, https://doi.org/10.1007/s12517- 015-1979-0. [36] R. T. Nickson, J. M. McArthur, B. Shrestha, T. O. Kyaw-Myint, and D. Lowry, "Arsenic and other drinking water quality issues, Muzaffargarh District, Pakistan," Applied Geochemistry, vol. 20, no. 1, pp. 55–68, Jan. 2005, https://doi.org/10.1016/j.apgeochem.2004.06.004. [37] M. A. Malana and M. A. Khosa, "Groundwater pollution with special focus on arsenic, Dera Ghazi Khan-Pakistan," Journal of Saudi Chemical Society, vol. 15, no. 1, pp. 39–47, Jan. 2011, https://doi.org/10.1016/ j.jscs.2010.09.009. [38] T. A. Chandio, M. N. Khan, M. T. Muhammad, O. Yalcinkaya, A. A. Wasim, and A. F. Kayis, "Fluoride and arsenic contamination in drinking water due to mining activities and its impact on local area population," Environmental Science and Pollution Research, vol. 28, no. 2, pp. 2355– 2368, Jan. 2021, https://doi.org/10.1007/s11356-020-10575-9. [39] P. Bhattacharya et al., "Health risk assessment of co-occurrence of toxic fluoride and arsenic in groundwater of Dharmanagar region, North Tripura (India)," Groundwater for Sustainable Development, vol. 11, Oct. 2020, Art. no. 100430, https://doi.org/10.1016/j.gsd.2020.100430. [40] M. Sanjrani, B. Zhou, H. Zhao, S. Bhutto, A. Muneer, and S. Xia, "Arsenic contaminated groundwater in China and its treatment options, a review," Applied Ecology and Environmental Research, vol. 17, no. 2, pp. 1655–1683, Feb. 2019, https://doi.org/10.15666/aeer/1702_ 16551683. [41] H. A. Jawadi, H. A. Malistani, M. A. Moheghy, and J. Sagin, "Essential Trace Elements and Arsenic in Thermal Springs, Afghanistan," Water, vol. 13, no. 2, Jan. 2021, Art. no. 134, https://doi.org/10.3390/ w13020134. [42] H.-J. Lin, T.-I. Sung, C.-Y. Chen, and H.-R. Guo, "Arsenic levels in drinking water and mortality of liver cancer in Taiwan," Journal of Hazardous Materials, vol. 262, pp. 1132–1138, Nov. 2013, https://doi.org/10.1016/j.jhazmat.2012.12.049. [43] E. Zkeri, M. Aloupi, and P. Gaganis, "Natural Occurrence of Arsenic in Groundwater from Lesvos Island, Greece," Water, Air, & Soil Pollution, vol. 226, no. 9, Aug. 2015, Art. no. 294, https://doi.org/10.1007/s11270- 015-2542-z. [44] N. Saha, Md. Bodrud-Doza, A. R. M. T. Islam, B. A. Begum, and M. S. Rahman, "Hydrogeochemical evolution of shallow and deeper aquifers in central Bangladesh: arsenic mobilization process and health risk implications from the potable use of groundwater," Environmental Earth Sciences, vol. 79, no. 20, Oct. 2020, Art. no. 477, https://doi.org/ 10.1007/s12665-020-09228-4. [45] M. Berg, H. C. Tran, T. C. Nguyen, H. V. Pham, R. Schertenleib, and W. Giger, "Arsenic Contamination of Groundwater and Drinking Water in Vietnam: A Human Health Threat," Environmental Science & Technology, vol. 35, no. 13, pp. 2621–2626, Jul. 2001, https://doi.org/ 10.1021/es010027y. [46] S. Gwachha, B. N. Acharya, A. Dhakal, S. M. Shrestha, and T. P. Joshi, "Assessment of Arsenic Content in Deep Groundwater of Kathmandu Valley, Nepal," Nepal Journal of Science and Technology, vol. 19, no. 1, pp. 69–77, Jul. 2020, https://doi.org/10.3126/njst.v19i1.29785. [47] K. Phan, S. Sthiannopkao, and K.-W. Kim, "Surveillance on chronic arsenic exposure in the Mekong River basin of Cambodia using different biomarkers," International Journal of Hygiene and Environmental Health, vol. 215, no. 1, pp. 51–58, Dec. 2011, https://doi.org/ 10.1016/j.ijheh.2011.07.002. [48] X. J. Guo, Y. Fujino, S. Kaneko, K. Wu, Y. Xia, and T. Yoshimura, "Arsenic contamination of groundwater and prevalence of arsenical dermatosis in the Hetao plain area, Inner Mongolia, China," in Molecular Mechanisms of Metal Toxicity and Carcinogenesis, X. Shi, V. Castranova, V. Vallyathan, and W. G. Perry, Eds. Boston, MA, USA: Springer, 2001, pp. 137–140. [49] N.-K. Bhatti, S. R. Samo, A. Saand, M. A. Keerio, and A. A. Bhuriro, "Ground Water Quality Assessment of Daur Taluka, Shaheed Benazir Abad," Engineering, Technology and Applied Science Research, vol. 8, no. 2, pp. 2785–2789, Apr. 2018, https://doi.org/10.48084/etasr.1925.