id sid tid token lemma pos eurjchem-1439 1 1 untitled untitle VERB eurjchem-1439 1 2 european european PROPN eurjchem-1439 1 3 journal journal PROPN eurjchem-1439 1 4 of of ADP eurjchem-1439 1 5 chemistry chemistry NOUN eurjchem-1439 1 6 7 7 NUM eurjchem-1439 1 7 ( ( PUNCT eurjchem-1439 1 8 3 3 NUM eurjchem-1439 1 9 ) ) PUNCT eurjchem-1439 1 10 ( ( PUNCT eurjchem-1439 1 11 2016 2016 NUM eurjchem-1439 1 12 ) ) PUNCT eurjchem-1439 1 13 375‐379 375‐379 PROPN eurjchem-1439 1 14 european european ADJ eurjchem-1439 1 15 journal journal PROPN eurjchem-1439 1 16 of of ADP eurjchem-1439 1 17 chemistry chemistry NOUN eurjchem-1439 1 18 issn issn PROPN eurjchem-1439 1 19 2153‐2249 2153‐2249 PROPN eurjchem-1439 1 20 ( ( PUNCT eurjchem-1439 1 21 print print NOUN eurjchem-1439 1 22 ) ) PUNCT eurjchem-1439 1 23 / / SYM eurjchem-1439 1 24 issn issn PROPN eurjchem-1439 1 25 2153‐2257 2153‐2257 NUM eurjchem-1439 1 26 ( ( PUNCT eurjchem-1439 1 27 online online ADJ eurjchem-1439 1 28 ) ) PUNCT eurjchem-1439 1 29   NOUN eurjchem-1439 1 30 2016 2016 NUM eurjchem-1439 1 31 atlanta atlanta PROPN eurjchem-1439 1 32 publishing publishing PROPN eurjchem-1439 1 33 house house PROPN eurjchem-1439 1 34 llc llc PROPN eurjchem-1439 1 35 ‐ ‐ PUNCT eurjchem-1439 1 36 all all DET eurjchem-1439 1 37 rights right NOUN eurjchem-1439 1 38 reserved reserve VERB eurjchem-1439 1 39 ‐ ‐ NOUN eurjchem-1439 1 40 printed print VERB eurjchem-1439 1 41 in in ADP eurjchem-1439 1 42 the the DET eurjchem-1439 1 43 usa usa PROPN eurjchem-1439 1 44 http://dx.doi.org/10.5155/eurjchem.7.3.375-379.1439 http://dx.doi.org/10.5155/eurjchem.7.3.375-379.1439 PROPN eurjchem-1439 1 45 european european PROPN eurjchem-1439 1 46 journal journal PROPN eurjchem-1439 1 47 of of ADP eurjchem-1439 1 48 chemistry chemistry PROPN eurjchem-1439 1 49 journal journal PROPN eurjchem-1439 1 50 webpage webpage NOUN eurjchem-1439 1 51 : : PUNCT eurjchem-1439 1 52 www.eurjchem.com www.eurjchem.com X eurjchem-1439 1 53 simple simple ADJ eurjchem-1439 1 54 chromatographic chromatographic ADJ eurjchem-1439 1 55 and and CCONJ eurjchem-1439 1 56 spectrophotometric spectrophotometric ADJ eurjchem-1439 1 57 determination determination NOUN eurjchem-1439 1 58 of of ADP eurjchem-1439 1 59 sofosbuvir sofosbuvir PROPN eurjchem-1439 1 60 in in ADP eurjchem-1439 1 61 pure pure ADJ eurjchem-1439 1 62 and and CCONJ eurjchem-1439 1 63 tablet tablet NOUN eurjchem-1439 1 64 forms form NOUN eurjchem-1439 1 65 sherif sherif VERB eurjchem-1439 1 66 abdel‐naby abdel‐naby PROPN eurjchem-1439 1 67 abdel‐gawad abdel‐gawad PROPN eurjchem-1439 2 1 1,2 1,2 NUM eurjchem-1439 2 2 , , PUNCT eurjchem-1439 2 3 * * SYM eurjchem-1439 2 4 1 1 NUM eurjchem-1439 2 5 pharmaceutical pharmaceutical NOUN eurjchem-1439 2 6 chemistry chemistry NOUN eurjchem-1439 2 7 department department NOUN eurjchem-1439 2 8 , , PUNCT eurjchem-1439 2 9 collage collage NOUN eurjchem-1439 2 10 of of ADP eurjchem-1439 2 11 pharmacy pharmacy NOUN eurjchem-1439 2 12 , , PUNCT eurjchem-1439 2 13 prince prince PROPN eurjchem-1439 2 14 sattam sattam PROPN eurjchem-1439 2 15 bin‐abdul bin‐abdul PROPN eurjchem-1439 2 16 aziz aziz PROPN eurjchem-1439 2 17 university university PROPN eurjchem-1439 2 18 , , PUNCT eurjchem-1439 2 19 al‐kharj al‐kharj VERB eurjchem-1439 2 20 , , PUNCT eurjchem-1439 2 21 119924 119924 NUM eurjchem-1439 2 22 , , PUNCT eurjchem-1439 2 23 kingdom kingdom NOUN eurjchem-1439 2 24 of of ADP eurjchem-1439 2 25 saudi saudi PROPN eurjchem-1439 2 26 arabia arabia PROPN eurjchem-1439 2 27 2 2 NUM eurjchem-1439 2 28 analytical analytical ADJ eurjchem-1439 2 29 chemistry chemistry NOUN eurjchem-1439 2 30 department department NOUN eurjchem-1439 2 31 , , PUNCT eurjchem-1439 2 32 faculty faculty NOUN eurjchem-1439 2 33 of of ADP eurjchem-1439 2 34 pharmacy pharmacy NOUN eurjchem-1439 2 35 , , PUNCT eurjchem-1439 2 36 cairo cairo PROPN eurjchem-1439 2 37 university university PROPN eurjchem-1439 2 38 , , PUNCT eurjchem-1439 2 39 cairo cairo PROPN eurjchem-1439 2 40 , , PUNCT eurjchem-1439 2 41 11562 11562 NUM eurjchem-1439 2 42 , , PUNCT eurjchem-1439 2 43 egypt egypt PROPN eurjchem-1439 2 44 * * PUNCT eurjchem-1439 2 45 corresponding correspond VERB eurjchem-1439 2 46 author author NOUN eurjchem-1439 2 47 at at ADP eurjchem-1439 2 48 : : PUNCT eurjchem-1439 2 49 analytical analytical ADJ eurjchem-1439 2 50 chemistry chemistry NOUN eurjchem-1439 2 51 department department NOUN eurjchem-1439 2 52 , , PUNCT eurjchem-1439 2 53 faculty faculty NOUN eurjchem-1439 2 54 of of ADP eurjchem-1439 2 55 pharmacy pharmacy NOUN eurjchem-1439 2 56 , , PUNCT eurjchem-1439 2 57 cairo cairo PROPN eurjchem-1439 2 58 university university PROPN eurjchem-1439 2 59 , , PUNCT eurjchem-1439 2 60 cairo cairo PROPN eurjchem-1439 2 61 , , PUNCT eurjchem-1439 2 62 11562 11562 NUM eurjchem-1439 2 63 , , PUNCT eurjchem-1439 2 64 egypt egypt PROPN eurjchem-1439 2 65 . . PUNCT eurjchem-1439 3 1 tel tel PROPN eurjchem-1439 3 2 . . PUNCT eurjchem-1439 3 3 : : PUNCT eurjchem-1439 4 1 +966.54.0586921 +966.54.0586921 PROPN eurjchem-1439 4 2 . . PUNCT eurjchem-1439 5 1 fax fax NOUN eurjchem-1439 5 2 : : PUNCT eurjchem-1439 5 3 +333333 +333333 NUM eurjchem-1439 5 4 . . PUNCT eurjchem-1439 5 5 e‐mail e‐mail PUNCT eurjchem-1439 6 1 address address NOUN eurjchem-1439 6 2 : : PUNCT eurjchem-1439 6 3 s.daif@psau.edu.sa s.daif@psau.edu.sa PROPN eurjchem-1439 6 4 ( ( PUNCT eurjchem-1439 6 5 s.a s.a PROPN eurjchem-1439 6 6 . . PROPN eurjchem-1439 6 7 abdel‐gawad abdel‐gawad PROPN eurjchem-1439 6 8 ) ) PUNCT eurjchem-1439 6 9 . . PUNCT eurjchem-1439 7 1 article article NOUN eurjchem-1439 7 2 information information PROPN eurjchem-1439 7 3 abstract abstract ADJ eurjchem-1439 7 4 doi doi PROPN eurjchem-1439 7 5 : : PUNCT eurjchem-1439 7 6     SPACE eurjchem-1439 7 7 10.5155 10.5155 NUM eurjchem-1439 7 8 / / SYM eurjchem-1439 7 9 eurjchem.7.3.375 eurjchem.7.3.375 PROPN eurjchem-1439 7 10 - - PUNCT eurjchem-1439 7 11 379.1439 379.1439 NUM eurjchem-1439 7 12 received receive VERB eurjchem-1439 7 13 : : PUNCT eurjchem-1439 7 14 14 14 NUM eurjchem-1439 7 15 april april PROPN eurjchem-1439 7 16 2016 2016 NUM eurjchem-1439 7 17 accepted accept VERB eurjchem-1439 7 18 : : PUNCT eurjchem-1439 7 19 07 07 NUM eurjchem-1439 7 20 may may PROPN eurjchem-1439 7 21 2016 2016 NUM eurjchem-1439 7 22 published publish VERB eurjchem-1439 7 23 online online ADV eurjchem-1439 7 24 : : PUNCT eurjchem-1439 7 25 30 30 NUM eurjchem-1439 7 26 september september PROPN eurjchem-1439 7 27 2016 2016 NUM eurjchem-1439 7 28 printed print VERB eurjchem-1439 7 29 : : PUNCT eurjchem-1439 7 30 30 30 NUM eurjchem-1439 7 31 september september PROPN eurjchem-1439 7 32 2016 2016 NUM eurjchem-1439 7 33     SPACE eurjchem-1439 7 34 two two NUM eurjchem-1439 7 35 methods method NOUN eurjchem-1439 7 36 , , PUNCT eurjchem-1439 7 37 a a DET eurjchem-1439 7 38 reversed reverse VERB eurjchem-1439 7 39 phase phase NOUN eurjchem-1439 7 40 high‐performance high‐performance NOUN eurjchem-1439 7 41 liquid liquid ADJ eurjchem-1439 7 42 chromatographic chromatographic ADJ eurjchem-1439 7 43 ( ( PUNCT eurjchem-1439 7 44 rp‐hplc rp‐hplc PROPN eurjchem-1439 7 45 ) ) PUNCT eurjchem-1439 7 46 method method NOUN eurjchem-1439 7 47 and and CCONJ eurjchem-1439 7 48 a a DET eurjchem-1439 7 49 direct direct ADJ eurjchem-1439 7 50 ultra‐violet ultra‐violet ADJ eurjchem-1439 7 51 spectrophotometric spectrophotometric ADJ eurjchem-1439 7 52 method method NOUN eurjchem-1439 7 53 , , PUNCT eurjchem-1439 7 54 were be AUX eurjchem-1439 7 55 adopted adopt VERB eurjchem-1439 7 56 and and CCONJ eurjchem-1439 7 57 validated validate VERB eurjchem-1439 7 58 for for ADP eurjchem-1439 7 59 the the DET eurjchem-1439 7 60 quantification quantification NOUN eurjchem-1439 7 61 of of ADP eurjchem-1439 7 62 sofosbuvir sofosbuvir PROPN eurjchem-1439 7 63 , , PUNCT eurjchem-1439 7 64 which which PRON eurjchem-1439 7 65 is be AUX eurjchem-1439 7 66 a a DET eurjchem-1439 7 67 new new ADJ eurjchem-1439 7 68 antiviral antiviral ADJ eurjchem-1439 7 69 agent agent NOUN eurjchem-1439 7 70 used use VERB eurjchem-1439 7 71 for for ADP eurjchem-1439 7 72 treatment treatment NOUN eurjchem-1439 7 73 of of ADP eurjchem-1439 7 74 patients patient NOUN eurjchem-1439 7 75 with with ADP eurjchem-1439 7 76 hepatitis hepatitis PROPN eurjchem-1439 7 77 c c PROPN eurjchem-1439 7 78 virus virus NOUN eurjchem-1439 7 79 ( ( PUNCT eurjchem-1439 7 80 hcv hcv PROPN eurjchem-1439 7 81 ) ) PUNCT eurjchem-1439 7 82 . . PUNCT eurjchem-1439 8 1 validation validation NOUN eurjchem-1439 8 2 parameters parameter NOUN eurjchem-1439 8 3 such such ADJ eurjchem-1439 8 4 as as ADP eurjchem-1439 8 5 linearity linearity NOUN eurjchem-1439 8 6 , , PUNCT eurjchem-1439 8 7 accuracy accuracy NOUN eurjchem-1439 8 8 , , PUNCT eurjchem-1439 8 9 precision precision NOUN eurjchem-1439 8 10 , , PUNCT eurjchem-1439 8 11 specificity specificity NOUN eurjchem-1439 8 12 , , PUNCT eurjchem-1439 8 13 limits limit NOUN eurjchem-1439 8 14 of of ADP eurjchem-1439 8 15 detection detection NOUN eurjchem-1439 8 16 and and CCONJ eurjchem-1439 8 17 quantification quantification NOUN eurjchem-1439 8 18 were be AUX eurjchem-1439 8 19 determined determine VERB eurjchem-1439 8 20 according accord VERB eurjchem-1439 8 21 to to ADP eurjchem-1439 8 22 the the DET eurjchem-1439 8 23 guidelines guideline NOUN eurjchem-1439 8 24 of of ADP eurjchem-1439 8 25 international international ADJ eurjchem-1439 8 26 conference conference NOUN eurjchem-1439 8 27 on on ADP eurjchem-1439 8 28 harmonization harmonization NOUN eurjchem-1439 8 29 ( ( PUNCT eurjchem-1439 8 30 ich)‐q2b ich)‐q2b PROPN eurjchem-1439 8 31 . . PUNCT eurjchem-1439 9 1 the the DET eurjchem-1439 9 2 rp‐hplc rp‐hplc PROPN eurjchem-1439 9 3 method method NOUN eurjchem-1439 9 4 was be AUX eurjchem-1439 9 5 applied apply VERB eurjchem-1439 9 6 on on ADP eurjchem-1439 9 7 hypersiltm hypersiltm PROPN eurjchem-1439 9 8 ods ods PROPN eurjchem-1439 9 9 c18 c18 NOUN eurjchem-1439 9 10 column column NOUN eurjchem-1439 9 11 ( ( PUNCT eurjchem-1439 9 12 150×4.6 150×4.6 NOUN eurjchem-1439 9 13 mm mm NOUN eurjchem-1439 9 14 , , PUNCT eurjchem-1439 9 15 5 5 NUM eurjchem-1439 9 16 µm µm NOUN eurjchem-1439 9 17 ) ) PUNCT eurjchem-1439 9 18 as as ADP eurjchem-1439 9 19 a a DET eurjchem-1439 9 20 stationary stationary ADJ eurjchem-1439 9 21 phase phase NOUN eurjchem-1439 9 22 . . PUNCT eurjchem-1439 10 1 the the DET eurjchem-1439 10 2 mobile mobile ADJ eurjchem-1439 10 3 phase phase NOUN eurjchem-1439 10 4 was be AUX eurjchem-1439 10 5 optimized optimize VERB eurjchem-1439 10 6 according accord VERB eurjchem-1439 10 7 to to ADP eurjchem-1439 10 8 the the DET eurjchem-1439 10 9 polarity polarity NOUN eurjchem-1439 10 10 of of ADP eurjchem-1439 10 11 the the DET eurjchem-1439 10 12 studied study VERB eurjchem-1439 10 13 drug drug NOUN eurjchem-1439 10 14 . . PUNCT eurjchem-1439 11 1 it it PRON eurjchem-1439 11 2 was be AUX eurjchem-1439 11 3 methanol methanol NOUN eurjchem-1439 11 4 : : PUNCT eurjchem-1439 11 5 acetonitrile acetonitrile NOUN eurjchem-1439 11 6 ( ( PUNCT eurjchem-1439 11 7 90:10 90:10 NUM eurjchem-1439 11 8 , , PUNCT eurjchem-1439 11 9 v v NOUN eurjchem-1439 11 10 : : SYM eurjchem-1439 11 11 v v NOUN eurjchem-1439 11 12 ) ) PUNCT eurjchem-1439 11 13 , , PUNCT eurjchem-1439 11 14 pumped pump VERB eurjchem-1439 11 15 using use VERB eurjchem-1439 11 16 an an DET eurjchem-1439 11 17 isocratic isocratic ADJ eurjchem-1439 11 18 mode mode NOUN eurjchem-1439 11 19 with with ADP eurjchem-1439 11 20 flow flow NOUN eurjchem-1439 11 21 rate rate NOUN eurjchem-1439 11 22 of of ADP eurjchem-1439 11 23 1 1 NUM eurjchem-1439 11 24 ml ml NOUN eurjchem-1439 11 25 / / SYM eurjchem-1439 11 26 min min NOUN eurjchem-1439 11 27 and and CCONJ eurjchem-1439 11 28 uv uv NOUN eurjchem-1439 11 29 detection detection NOUN eurjchem-1439 11 30 at at ADP eurjchem-1439 11 31 260 260 NUM eurjchem-1439 11 32 nm nm NOUN eurjchem-1439 11 33 . . PUNCT eurjchem-1439 12 1 the the DET eurjchem-1439 12 2 uv uv NOUN eurjchem-1439 12 3 spectrophotometric spectrophotometric ADJ eurjchem-1439 12 4 method method NOUN eurjchem-1439 12 5 was be AUX eurjchem-1439 12 6 performed perform VERB eurjchem-1439 12 7 for for ADP eurjchem-1439 12 8 the the DET eurjchem-1439 12 9 studied study VERB eurjchem-1439 12 10 drug drug NOUN eurjchem-1439 12 11 at at ADP eurjchem-1439 12 12 260 260 NUM eurjchem-1439 12 13 nm nm NOUN eurjchem-1439 12 14 . . PUNCT eurjchem-1439 13 1 the the DET eurjchem-1439 13 2 calibration calibration NOUN eurjchem-1439 13 3 curves curve NOUN eurjchem-1439 13 4 were be AUX eurjchem-1439 13 5 linear linear ADJ eurjchem-1439 13 6 in in ADP eurjchem-1439 13 7 the the DET eurjchem-1439 13 8 ranges range NOUN eurjchem-1439 13 9 of of ADP eurjchem-1439 13 10 2‐60 2‐60 NUM eurjchem-1439 13 11 µg µg NOUN eurjchem-1439 13 12 / / SYM eurjchem-1439 13 13 ml ml NOUN eurjchem-1439 13 14 and and CCONJ eurjchem-1439 13 15 5‐40 5‐40 NUM eurjchem-1439 13 16 µg µg ADJ eurjchem-1439 13 17 / / SYM eurjchem-1439 13 18 ml ml NOUN eurjchem-1439 13 19 for for ADP eurjchem-1439 13 20 the the DET eurjchem-1439 13 21 rp‐hplc rp‐hplc PROPN eurjchem-1439 13 22 and and CCONJ eurjchem-1439 13 23 uv uv NOUN eurjchem-1439 13 24 spectrophotometric spectrophotometric ADJ eurjchem-1439 13 25 methods method NOUN eurjchem-1439 13 26 , , PUNCT eurjchem-1439 13 27 respectively respectively ADV eurjchem-1439 13 28 . . PUNCT eurjchem-1439 14 1 the the DET eurjchem-1439 14 2 proposed propose VERB eurjchem-1439 14 3 methods method NOUN eurjchem-1439 14 4 are be AUX eurjchem-1439 14 5 accurate accurate ADJ eurjchem-1439 14 6 , , PUNCT eurjchem-1439 14 7 sensitive sensitive ADJ eurjchem-1439 14 8 and and CCONJ eurjchem-1439 14 9 precise precise ADJ eurjchem-1439 14 10 , , PUNCT eurjchem-1439 14 11 so so SCONJ eurjchem-1439 14 12 they they PRON eurjchem-1439 14 13 can can AUX eurjchem-1439 14 14 be be AUX eurjchem-1439 14 15 successfully successfully ADV eurjchem-1439 14 16 adopted adopt VERB eurjchem-1439 14 17 for for ADP eurjchem-1439 14 18 the the DET eurjchem-1439 14 19 reliable reliable ADJ eurjchem-1439 14 20 determination determination NOUN eurjchem-1439 14 21 of of ADP eurjchem-1439 14 22 sofosbuvir sofosbuvir PROPN eurjchem-1439 14 23 content content PROPN eurjchem-1439 14 24 in in ADP eurjchem-1439 14 25 its its PRON eurjchem-1439 14 26 tablet tablet NOUN eurjchem-1439 14 27 form form NOUN eurjchem-1439 14 28 . . PUNCT eurjchem-1439 15 1 keywords keyword NOUN eurjchem-1439 15 2 rp‐hplc rp‐hplc VERB eurjchem-1439 15 3 validation validation NOUN eurjchem-1439 15 4 sofosbuvir sofosbuvir PROPN eurjchem-1439 15 5 antiviral antiviral ADJ eurjchem-1439 15 6 drug drug PROPN eurjchem-1439 15 7 analytical analytical ADJ eurjchem-1439 15 8 methods method NOUN eurjchem-1439 15 9 uv uv INTJ eurjchem-1439 15 10 / / SYM eurjchem-1439 15 11 vis vis X eurjchem-1439 15 12 spectroscopy spectroscopy NOUN eurjchem-1439 15 13 cite cite VERB eurjchem-1439 15 14 this this PRON eurjchem-1439 15 15 : : PUNCT eurjchem-1439 15 16 eur eur PROPN eurjchem-1439 15 17 . . PUNCT eurjchem-1439 16 1 j. j. PROPN eurjchem-1439 16 2 chem chem PROPN eurjchem-1439 16 3 . . PUNCT eurjchem-1439 17 1 2016 2016 NUM eurjchem-1439 17 2 , , PUNCT eurjchem-1439 17 3 7(3 7(3 NUM eurjchem-1439 17 4 ) ) PUNCT eurjchem-1439 17 5 , , PUNCT eurjchem-1439 17 6 375‐379 375‐379 NUM eurjchem-1439 17 7 1 1 NUM eurjchem-1439 17 8 . . PUNCT eurjchem-1439 17 9 introduction introduction NOUN eurjchem-1439 17 10 sofosbuvir sofosbuvir PROPN eurjchem-1439 17 11 ( ( PUNCT eurjchem-1439 17 12 sfv sfv PROPN eurjchem-1439 17 13 ) ) PUNCT eurjchem-1439 17 14 ( ( PUNCT eurjchem-1439 17 15 isopropyl isopropyl NOUN eurjchem-1439 17 16 ( ( PUNCT eurjchem-1439 17 17 2s)‐2‐[[[(2r,3r,4r,5r)‐5‐(2 2s)‐2‐[[[(2r,3r,4r,5r)‐5‐(2 PROPN eurjchem-1439 17 18 , , PUNCT eurjchem-1439 17 19 4‐dioxopyrimidin‐1‐yl)‐4‐fluoro‐3‐hydroxy‐4‐methyl‐tetra 4‐dioxopyrimidin‐1‐yl)‐4‐fluoro‐3‐hydroxy‐4‐methyl‐tetra PROPN eurjchem-1439 17 20 hydrofuran‐2‐yl hydrofuran‐2‐yl NOUN eurjchem-1439 17 21 ] ] X eurjchem-1439 17 22 methoxy‐phenoxy‐phosphoryl methoxy‐phenoxy‐phosphoryl ADJ eurjchem-1439 17 23 ] ] X eurjchem-1439 17 24 amino amino NOUN eurjchem-1439 17 25 ] ] X eurjchem-1439 17 26 pro‐ pro‐ PROPN eurjchem-1439 17 27 panoate panoate NOUN eurjchem-1439 17 28 ) ) PUNCT eurjchem-1439 17 29 is be AUX eurjchem-1439 17 30 a a DET eurjchem-1439 17 31 nucleotide nucleotide NOUN eurjchem-1439 17 32 prodrug prodrug NOUN eurjchem-1439 17 33 analog analog NOUN eurjchem-1439 17 34 that that PRON eurjchem-1439 17 35 can can AUX eurjchem-1439 17 36 be be AUX eurjchem-1439 17 37 used use VERB eurjchem-1439 17 38 for for ADP eurjchem-1439 17 39 treatment treatment NOUN eurjchem-1439 17 40 of of ADP eurjchem-1439 17 41 patients patient NOUN eurjchem-1439 17 42 with with ADP eurjchem-1439 17 43 hepatitis hepatitis PROPN eurjchem-1439 17 44 c c PROPN eurjchem-1439 17 45 virus virus NOUN eurjchem-1439 17 46 ( ( PUNCT eurjchem-1439 17 47 hcv hcv PROPN eurjchem-1439 17 48 ) ) PUNCT eurjchem-1439 17 49 ( ( PUNCT eurjchem-1439 17 50 scheme scheme NOUN eurjchem-1439 17 51 1 1 NUM eurjchem-1439 17 52 ) ) PUNCT eurjchem-1439 18 1 [ [ X eurjchem-1439 18 2 1 1 NUM eurjchem-1439 18 3 ] ] PUNCT eurjchem-1439 18 4 . . PUNCT eurjchem-1439 19 1 it it PRON eurjchem-1439 19 2 can can AUX eurjchem-1439 19 3 be be AUX eurjchem-1439 19 4 metabolized metabolize VERB eurjchem-1439 19 5 to to ADP eurjchem-1439 19 6 the the DET eurjchem-1439 19 7 pharmacologically pharmacologically ADV eurjchem-1439 19 8 active active ADJ eurjchem-1439 19 9 uridine uridine NOUN eurjchem-1439 19 10 analog analog NOUN eurjchem-1439 19 11 triphosphate triphosphate NOUN eurjchem-1439 19 12 . . PUNCT eurjchem-1439 20 1 this this DET eurjchem-1439 20 2 metabolite metabolite NOUN eurjchem-1439 20 3 can can AUX eurjchem-1439 20 4 be be AUX eurjchem-1439 20 5 incorporated incorporate VERB eurjchem-1439 20 6 into into ADP eurjchem-1439 20 7 the the DET eurjchem-1439 20 8 ribonucleic ribonucleic ADJ eurjchem-1439 20 9 acid acid NOUN eurjchem-1439 20 10 ( ( PUNCT eurjchem-1439 20 11 rna rna PROPN eurjchem-1439 20 12 ) ) PUNCT eurjchem-1439 20 13 of of ADP eurjchem-1439 20 14 the the DET eurjchem-1439 20 15 hcv hcv NOUN eurjchem-1439 20 16 and and CCONJ eurjchem-1439 20 17 so so ADV eurjchem-1439 20 18 acts act VERB eurjchem-1439 20 19 as as ADP eurjchem-1439 20 20 a a DET eurjchem-1439 20 21 chain chain NOUN eurjchem-1439 20 22 terminator terminator NOUN eurjchem-1439 20 23 . . PUNCT eurjchem-1439 21 1 the the DET eurjchem-1439 21 2 termination termination NOUN eurjchem-1439 21 3 of of ADP eurjchem-1439 21 4 rna rna NOUN eurjchem-1439 21 5 synthesis synthesis NOUN eurjchem-1439 21 6 prevents prevent VERB eurjchem-1439 21 7 replication replication NOUN eurjchem-1439 21 8 of of ADP eurjchem-1439 21 9 the the DET eurjchem-1439 21 10 virus virus NOUN eurjchem-1439 21 11 and and CCONJ eurjchem-1439 21 12 so so ADV eurjchem-1439 21 13 leads lead VERB eurjchem-1439 21 14 to to ADP eurjchem-1439 21 15 a a DET eurjchem-1439 21 16 rapid rapid ADJ eurjchem-1439 21 17 decrease decrease NOUN eurjchem-1439 21 18 of of ADP eurjchem-1439 21 19 hcv hcv X eurjchem-1439 21 20 viral viral ADJ eurjchem-1439 21 21 count count NOUN eurjchem-1439 22 1 [ [ X eurjchem-1439 22 2 2 2 NUM eurjchem-1439 22 3 ] ] PUNCT eurjchem-1439 22 4 . . PUNCT eurjchem-1439 23 1 sfv sfv PROPN eurjchem-1439 23 2 can can AUX eurjchem-1439 23 3 be be AUX eurjchem-1439 23 4 used use VERB eurjchem-1439 23 5 alone alone ADJ eurjchem-1439 23 6 or or CCONJ eurjchem-1439 23 7 in in ADP eurjchem-1439 23 8 combination combination NOUN eurjchem-1439 23 9 with with ADP eurjchem-1439 23 10 other other ADJ eurjchem-1439 23 11 drugs drug NOUN eurjchem-1439 23 12 like like ADP eurjchem-1439 23 13 ribavarin ribavarin NOUN eurjchem-1439 23 14 and and CCONJ eurjchem-1439 23 15 ledipasvir ledipasvir NOUN eurjchem-1439 24 1 [ [ X eurjchem-1439 24 2 3 3 NUM eurjchem-1439 24 3 ] ] PUNCT eurjchem-1439 24 4 . . PUNCT eurjchem-1439 25 1 sfv sfv PROPN eurjchem-1439 25 2 has have AUX eurjchem-1439 25 3 been be AUX eurjchem-1439 25 4 included include VERB eurjchem-1439 25 5 in in ADP eurjchem-1439 25 6 the the DET eurjchem-1439 25 7 essential essential ADJ eurjchem-1439 25 8 medicines medicine NOUN eurjchem-1439 25 9 list list NOUN eurjchem-1439 25 10 of of ADP eurjchem-1439 25 11 world world NOUN eurjchem-1439 25 12 health health NOUN eurjchem-1439 25 13 organization organization NOUN eurjchem-1439 25 14 ( ( PUNCT eurjchem-1439 25 15 who who PRON eurjchem-1439 25 16 ) ) PUNCT eurjchem-1439 25 17 which which PRON eurjchem-1439 25 18 shows show VERB eurjchem-1439 25 19 the the DET eurjchem-1439 25 20 high high ADJ eurjchem-1439 25 21 potentiality potentiality NOUN eurjchem-1439 25 22 of of ADP eurjchem-1439 25 23 the the DET eurjchem-1439 25 24 drug drug NOUN eurjchem-1439 25 25 in in ADP eurjchem-1439 25 26 the the DET eurjchem-1439 25 27 treatment treatment NOUN eurjchem-1439 25 28 regimen regiman NOUN eurjchem-1439 25 29 of of ADP eurjchem-1439 25 30 hcv hcv NOUN eurjchem-1439 25 31 [ [ X eurjchem-1439 25 32 4 4 NUM eurjchem-1439 25 33 ] ] PUNCT eurjchem-1439 25 34 . . PUNCT eurjchem-1439 26 1 there there PRON eurjchem-1439 26 2 is be VERB eurjchem-1439 26 3 no no PRON eurjchem-1439 26 4 much much ADV eurjchem-1439 26 5 reported report VERB eurjchem-1439 26 6 literature literature NOUN eurjchem-1439 26 7 concerning concern VERB eurjchem-1439 26 8 the the DET eurjchem-1439 26 9 quantification quantification NOUN eurjchem-1439 26 10 of of ADP eurjchem-1439 26 11 sfv sfv NOUN eurjchem-1439 26 12 due due ADP eurjchem-1439 26 13 to to ADP eurjchem-1439 26 14 the the DET eurjchem-1439 26 15 recent recent ADJ eurjchem-1439 26 16 launch launch NOUN eurjchem-1439 26 17 of of ADP eurjchem-1439 26 18 the the DET eurjchem-1439 26 19 drug drug NOUN eurjchem-1439 26 20 in in ADP eurjchem-1439 26 21 the the DET eurjchem-1439 26 22 market market NOUN eurjchem-1439 26 23 ( ( PUNCT eurjchem-1439 26 24 2013 2013 NUM eurjchem-1439 26 25 ) ) PUNCT eurjchem-1439 26 26 . . PUNCT eurjchem-1439 27 1 all all DET eurjchem-1439 27 2 the the DET eurjchem-1439 27 3 performed perform VERB eurjchem-1439 27 4 methods method NOUN eurjchem-1439 27 5 used use VERB eurjchem-1439 27 6 the the DET eurjchem-1439 27 7 ultra‐ ultra‐ PROPN eurjchem-1439 27 8 performance performance NOUN eurjchem-1439 27 9 liquid liquid ADJ eurjchem-1439 27 10 chromatography chromatography NOUN eurjchem-1439 27 11 ‐ ‐ ADP eurjchem-1439 27 12 tandem tandem PROPN eurjchem-1439 27 13 mass mass ADJ eurjchem-1439 27 14 spectros‐ spectros‐ NOUN eurjchem-1439 27 15 copy copy NOUN eurjchem-1439 27 16 ( ( PUNCT eurjchem-1439 27 17 uplc‐ms uplc‐ms NOUN eurjchem-1439 27 18 / / SYM eurjchem-1439 27 19 ms ms NOUN eurjchem-1439 27 20 ) ) PUNCT eurjchem-1439 27 21 technique technique NOUN eurjchem-1439 27 22 for for ADP eurjchem-1439 27 23 the the DET eurjchem-1439 27 24 studied study VERB eurjchem-1439 27 25 drug drug NOUN eurjchem-1439 27 26 deter‐ deter‐ AUX eurjchem-1439 27 27 mination mination VERB eurjchem-1439 27 28 with with ADP eurjchem-1439 27 29 other other ADJ eurjchem-1439 27 30 antiviral antiviral ADJ eurjchem-1439 27 31 drugs drug NOUN eurjchem-1439 27 32 like like ADP eurjchem-1439 27 33 ribavirin ribavirin NOUN eurjchem-1439 27 34 , , PUNCT eurjchem-1439 27 35 ledipasvir ledipasvir PROPN eurjchem-1439 27 36 or or CCONJ eurjchem-1439 27 37 with with ADP eurjchem-1439 27 38 its its PRON eurjchem-1439 27 39 metabolite metabolite NOUN eurjchem-1439 27 40 [ [ X eurjchem-1439 27 41 5‐7 5‐7 NUM eurjchem-1439 27 42 ] ] X eurjchem-1439 27 43 . . PUNCT eurjchem-1439 28 1 scheme scheme NOUN eurjchem-1439 28 2 1 1 NUM eurjchem-1439 28 3 from from ADP eurjchem-1439 28 4 the the DET eurjchem-1439 28 5 previous previous ADJ eurjchem-1439 28 6 literature literature NOUN eurjchem-1439 28 7 review review NOUN eurjchem-1439 28 8 , , PUNCT eurjchem-1439 28 9 there there PRON eurjchem-1439 28 10 is be VERB eurjchem-1439 28 11 an an DET eurjchem-1439 28 12 urgent urgent ADJ eurjchem-1439 28 13 need need NOUN eurjchem-1439 28 14 for for ADP eurjchem-1439 28 15 a a DET eurjchem-1439 28 16 simple simple ADJ eurjchem-1439 28 17 , , PUNCT eurjchem-1439 28 18 accurate accurate ADJ eurjchem-1439 28 19 and and CCONJ eurjchem-1439 28 20 economic economic ADJ eurjchem-1439 28 21 analytical analytical ADJ eurjchem-1439 28 22 method method NOUN eurjchem-1439 28 23 for for ADP eurjchem-1439 28 24 the the DET eurjchem-1439 28 25 rapid rapid ADJ eurjchem-1439 28 26 quantification quantification NOUN eurjchem-1439 28 27 of of ADP eurjchem-1439 28 28 sfv sfv PROPN eurjchem-1439 28 29 either either CCONJ eurjchem-1439 28 30 in in ADP eurjchem-1439 28 31 pure pure ADJ eurjchem-1439 28 32 form form NOUN eurjchem-1439 28 33 or or CCONJ eurjchem-1439 28 34 in in ADP eurjchem-1439 28 35 its its PRON eurjchem-1439 28 36 tablet tablet NOUN eurjchem-1439 28 37 form form NOUN eurjchem-1439 28 38 as as SCONJ eurjchem-1439 28 39 all all DET eurjchem-1439 28 40 the the DET eurjchem-1439 28 41 published publish VERB eurjchem-1439 28 42 methods method NOUN eurjchem-1439 28 43 used use VERB eurjchem-1439 28 44 a a DET eurjchem-1439 28 45 complicated complicated ADJ eurjchem-1439 28 46 expensive expensive ADJ eurjchem-1439 28 47 technique technique NOUN eurjchem-1439 28 48 ( ( PUNCT eurjchem-1439 28 49 uplc‐ms uplc‐ms NOUN eurjchem-1439 28 50 / / SYM eurjchem-1439 28 51 ms ms NOUN eurjchem-1439 28 52 ) ) PUNCT eurjchem-1439 28 53 . . PUNCT eurjchem-1439 29 1 the the DET eurjchem-1439 29 2 target target NOUN eurjchem-1439 29 3 of of ADP eurjchem-1439 29 4 the the DET eurjchem-1439 29 5 present present ADJ eurjchem-1439 29 6 work work NOUN eurjchem-1439 29 7 is be AUX eurjchem-1439 29 8 to to PART eurjchem-1439 29 9 apply apply VERB eurjchem-1439 29 10 and and CCONJ eurjchem-1439 29 11 validate validate VERB eurjchem-1439 29 12 analytical analytical ADJ eurjchem-1439 29 13 methods method NOUN eurjchem-1439 29 14 characterized characterize VERB eurjchem-1439 29 15 with with ADP eurjchem-1439 29 16 accuracy accuracy NOUN eurjchem-1439 29 17 and and CCONJ eurjchem-1439 29 18 simplicity simplicity NOUN eurjchem-1439 29 19 like like ADP eurjchem-1439 29 20 rp‐hplc rp‐hplc PROPN eurjchem-1439 29 21 and and CCONJ eurjchem-1439 29 22 uv uv NOUN eurjchem-1439 29 23 spectro‐ spectro‐ NOUN eurjchem-1439 29 24 photometry photometry NOUN eurjchem-1439 29 25 for for ADP eurjchem-1439 29 26 the the DET eurjchem-1439 29 27 quantification quantification NOUN eurjchem-1439 29 28 of of ADP eurjchem-1439 29 29 the the DET eurjchem-1439 29 30 studied study VERB eurjchem-1439 29 31 drug drug NOUN eurjchem-1439 29 32 either either CCONJ eurjchem-1439 29 33 in in ADP eurjchem-1439 29 34 pure pure ADJ eurjchem-1439 29 35 or or CCONJ eurjchem-1439 29 36 in in ADP eurjchem-1439 29 37 dosage dosage NOUN eurjchem-1439 29 38 forms form NOUN eurjchem-1439 29 39 and and CCONJ eurjchem-1439 29 40 can can AUX eurjchem-1439 29 41 be be AUX eurjchem-1439 29 42 applied apply VERB eurjchem-1439 29 43 for for ADP eurjchem-1439 29 44 the the DET eurjchem-1439 29 45 rapid rapid ADJ eurjchem-1439 29 46 estimation estimation NOUN eurjchem-1439 29 47 of of ADP eurjchem-1439 29 48 sfv sfv PROPN eurjchem-1439 29 49 in in ADP eurjchem-1439 29 50 quality quality NOUN eurjchem-1439 29 51 control control NOUN eurjchem-1439 29 52 laboratories laboratory NOUN eurjchem-1439 29 53 . . PUNCT eurjchem-1439 30 1 376 376 NUM eurjchem-1439 30 2 sherif sherif VERB eurjchem-1439 30 3 abdel‐naby abdel‐naby PROPN eurjchem-1439 30 4 abdel‐gawad abdel‐gawad PROPN eurjchem-1439 30 5 / / SYM eurjchem-1439 30 6 european european PROPN eurjchem-1439 30 7 journal journal PROPN eurjchem-1439 30 8 of of ADP eurjchem-1439 30 9 chemistry chemistry NOUN eurjchem-1439 30 10 7 7 NUM eurjchem-1439 30 11 ( ( PUNCT eurjchem-1439 30 12 3 3 NUM eurjchem-1439 30 13 ) ) PUNCT eurjchem-1439 30 14 ( ( PUNCT eurjchem-1439 30 15 2016 2016 NUM eurjchem-1439 30 16 ) ) PUNCT eurjchem-1439 30 17 375‐379 375‐379 NUM eurjchem-1439 30 18 2 2 NUM eurjchem-1439 30 19 . . PUNCT eurjchem-1439 30 20 experimental experimental ADJ eurjchem-1439 30 21 2.1 2.1 NUM eurjchem-1439 30 22 . . PUNCT eurjchem-1439 30 23 chemicals chemical NOUN eurjchem-1439 30 24 and and CCONJ eurjchem-1439 30 25 reagents reagent NOUN eurjchem-1439 30 26 pure pure ADJ eurjchem-1439 30 27 sofosbuvir sofosbuvir NOUN eurjchem-1439 30 28 ( ( PUNCT eurjchem-1439 30 29 psi‐7977 psi‐7977 NOUN eurjchem-1439 30 30 ) ) PUNCT eurjchem-1439 30 31 was be AUX eurjchem-1439 30 32 purchased purchase VERB eurjchem-1439 30 33 from from ADP eurjchem-1439 30 34 cayman cayman PROPN eurjchem-1439 30 35 chemical chemical PROPN eurjchem-1439 30 36 company company NOUN eurjchem-1439 30 37 , , PUNCT eurjchem-1439 30 38 ann ann PROPN eurjchem-1439 30 39 arbor arbor PROPN eurjchem-1439 30 40 , , PUNCT eurjchem-1439 30 41 united united PROPN eurjchem-1439 30 42 states states PROPN eurjchem-1439 30 43 of of ADP eurjchem-1439 30 44 america america PROPN eurjchem-1439 30 45 ( ( PUNCT eurjchem-1439 30 46 usa usa PROPN eurjchem-1439 30 47 ) ) PUNCT eurjchem-1439 30 48 ; ; PUNCT eurjchem-1439 30 49 its its PRON eurjchem-1439 30 50 purity purity NOUN eurjchem-1439 30 51 was be AUX eurjchem-1439 30 52 certified certify VERB eurjchem-1439 30 53 to to PART eurjchem-1439 30 54 be be AUX eurjchem-1439 30 55 99.9 99.9 NUM eurjchem-1439 30 56 % % NOUN eurjchem-1439 30 57 . . PUNCT eurjchem-1439 31 1 acetonitrile acetonitrile NOUN eurjchem-1439 31 2 and and CCONJ eurjchem-1439 31 3 methanol methanol NOUN eurjchem-1439 31 4 ( ( PUNCT eurjchem-1439 31 5 hplc hplc NOUN eurjchem-1439 31 6 grade grade NOUN eurjchem-1439 31 7 ) ) PUNCT eurjchem-1439 31 8 were be AUX eurjchem-1439 31 9 supplied supply VERB eurjchem-1439 31 10 by by ADP eurjchem-1439 31 11 sigma sigma PROPN eurjchem-1439 31 12 aldrich aldrich PROPN eurjchem-1439 31 13 , , PUNCT eurjchem-1439 31 14 st st PROPN eurjchem-1439 31 15 . . PROPN eurjchem-1439 31 16 louis louis PROPN eurjchem-1439 31 17 , , PUNCT eurjchem-1439 31 18 usa usa PROPN eurjchem-1439 31 19 . . PROPN eurjchem-1439 31 20 2.2 2.2 NUM eurjchem-1439 31 21 . . PUNCT eurjchem-1439 32 1 pharmaceutical pharmaceutical ADJ eurjchem-1439 32 2 formulation formulation NOUN eurjchem-1439 32 3 sovaldi sovaldi NOUN eurjchem-1439 32 4 ® ® NOUN eurjchem-1439 32 5 400 400 NUM eurjchem-1439 32 6 mg mg NOUN eurjchem-1439 32 7 film film NOUN eurjchem-1439 32 8 coated coat VERB eurjchem-1439 32 9 tablets tablet NOUN eurjchem-1439 32 10 with with ADP eurjchem-1439 32 11 batch batch NOUN eurjchem-1439 32 12 number number NOUN eurjchem-1439 32 13 pmpw pmpw NOUN eurjchem-1439 32 14 . . PUNCT eurjchem-1439 33 1 it it PRON eurjchem-1439 33 2 was be AUX eurjchem-1439 33 3 manufactured manufacture VERB eurjchem-1439 33 4 by by ADP eurjchem-1439 33 5 gilead gilead PROPN eurjchem-1439 33 6 sciences sciences PROPN eurjchem-1439 33 7 , , PUNCT eurjchem-1439 33 8 inc inc PROPN eurjchem-1439 33 9 . . PROPN eurjchem-1439 33 10 foster foster PROPN eurjchem-1439 33 11 city city PROPN eurjchem-1439 33 12 , , PUNCT eurjchem-1439 33 13 usa usa PROPN eurjchem-1439 33 14 . . PROPN eurjchem-1439 33 15 2.3 2.3 NUM eurjchem-1439 33 16 . . PUNCT eurjchem-1439 34 1 instrumentation instrumentation NOUN eurjchem-1439 34 2 the the DET eurjchem-1439 34 3 liquid liquid ADJ eurjchem-1439 34 4 chromatographic chromatographic ADJ eurjchem-1439 34 5 method method NOUN eurjchem-1439 34 6 was be AUX eurjchem-1439 34 7 performed perform VERB eurjchem-1439 34 8 on on ADP eurjchem-1439 34 9 high high ADJ eurjchem-1439 34 10 performance performance NOUN eurjchem-1439 34 11 liquid liquid ADJ eurjchem-1439 34 12 chromatograph chromatograph NOUN eurjchem-1439 34 13 consisted consist VERB eurjchem-1439 34 14 of of ADP eurjchem-1439 34 15 a a DET eurjchem-1439 34 16 binary binary ADJ eurjchem-1439 34 17 pump pump NOUN eurjchem-1439 34 18 ( ( PUNCT eurjchem-1439 34 19 waters water NOUN eurjchem-1439 34 20 , , PUNCT eurjchem-1439 34 21 1525 1525 NUM eurjchem-1439 34 22 ) ) PUNCT eurjchem-1439 34 23 , , PUNCT eurjchem-1439 34 24 a a DET eurjchem-1439 34 25 uv‐visible uv‐visible ADJ eurjchem-1439 34 26 wavelength wavelength NOUN eurjchem-1439 34 27 detector detector NOUN eurjchem-1439 34 28 ( ( PUNCT eurjchem-1439 34 29 waters water NOUN eurjchem-1439 34 30 , , PUNCT eurjchem-1439 34 31 2489 2489 NUM eurjchem-1439 34 32 ) ) PUNCT eurjchem-1439 34 33 and and CCONJ eurjchem-1439 34 34 an an DET eurjchem-1439 34 35 auto‐sampler auto‐sampler NOUN eurjchem-1439 34 36 ( ( PUNCT eurjchem-1439 34 37 waters water NOUN eurjchem-1439 34 38 , , PUNCT eurjchem-1439 34 39 2707 2707 NUM eurjchem-1439 34 40 ) ) PUNCT eurjchem-1439 34 41 . . PUNCT eurjchem-1439 35 1 the the DET eurjchem-1439 35 2 chromatograph chromatograph NOUN eurjchem-1439 35 3 is be AUX eurjchem-1439 35 4 a a DET eurjchem-1439 35 5 product product NOUN eurjchem-1439 35 6 of of ADP eurjchem-1439 35 7 waters water NOUN eurjchem-1439 35 8 , , PUNCT eurjchem-1439 35 9 massachusetts massachusetts PROPN eurjchem-1439 35 10 , , PUNCT eurjchem-1439 35 11 usa usa PROPN eurjchem-1439 35 12 . . PUNCT eurjchem-1439 36 1 the the DET eurjchem-1439 36 2 spectrophotometric spectrophotometric ADJ eurjchem-1439 36 3 analysis analysis NOUN eurjchem-1439 36 4 was be AUX eurjchem-1439 36 5 done do VERB eurjchem-1439 36 6 using use VERB eurjchem-1439 36 7 double double ADJ eurjchem-1439 36 8 beam beam NOUN eurjchem-1439 36 9 spectrophotometer spectrophotometer NOUN eurjchem-1439 36 10 ( ( PUNCT eurjchem-1439 36 11 jasco jasco PROPN eurjchem-1439 36 12 , , PUNCT eurjchem-1439 36 13 japan japan PROPN eurjchem-1439 36 14 ) ) PUNCT eurjchem-1439 36 15 with with ADP eurjchem-1439 36 16 1 1 NUM eurjchem-1439 36 17 cm cm NOUN eurjchem-1439 36 18 path path NOUN eurjchem-1439 36 19 length length NOUN eurjchem-1439 36 20 matched match VERB eurjchem-1439 36 21 quartz quartz NOUN eurjchem-1439 36 22 cuvettes cuvette NOUN eurjchem-1439 36 23 . . PUNCT eurjchem-1439 37 1 it it PRON eurjchem-1439 37 2 is be AUX eurjchem-1439 37 3 connected connect VERB eurjchem-1439 37 4 to to ADP eurjchem-1439 37 5 ibm ibm PROPN eurjchem-1439 37 6 compatible compatible ADJ eurjchem-1439 37 7 computer computer NOUN eurjchem-1439 37 8 with with ADP eurjchem-1439 37 9 hp hp NOUN eurjchem-1439 37 10 680 680 NUM eurjchem-1439 37 11 inkjet inkjet NOUN eurjchem-1439 37 12 printer printer NOUN eurjchem-1439 37 13 ( ( PUNCT eurjchem-1439 37 14 hewlett hewlett PROPN eurjchem-1439 37 15 packard packard PROPN eurjchem-1439 37 16 , , PUNCT eurjchem-1439 37 17 usa usa PROPN eurjchem-1439 37 18 ) ) PUNCT eurjchem-1439 37 19 . . PUNCT eurjchem-1439 38 1 2.4 2.4 NUM eurjchem-1439 38 2 . . PUNCT eurjchem-1439 39 1 standard standard ADJ eurjchem-1439 39 2 solutions solution NOUN eurjchem-1439 39 3 stock stock NOUN eurjchem-1439 39 4 standard standard ADJ eurjchem-1439 39 5 solution solution NOUN eurjchem-1439 39 6 ( ( PUNCT eurjchem-1439 39 7 1 1 NUM eurjchem-1439 39 8 mg mg NUM eurjchem-1439 39 9 / / SYM eurjchem-1439 39 10 ml ml NOUN eurjchem-1439 39 11 ) ) PUNCT eurjchem-1439 39 12 was be AUX eurjchem-1439 39 13 prepared prepare VERB eurjchem-1439 39 14 by by ADP eurjchem-1439 39 15 accurate accurate ADJ eurjchem-1439 39 16 weighing weighing NOUN eurjchem-1439 39 17 and and CCONJ eurjchem-1439 39 18 transferring transferring NOUN eurjchem-1439 39 19 of of ADP eurjchem-1439 39 20 50 50 NUM eurjchem-1439 39 21 mg mg NOUN eurjchem-1439 39 22 of of ADP eurjchem-1439 39 23 pure pure ADJ eurjchem-1439 39 24 sfv sfv NOUN eurjchem-1439 39 25 into into ADP eurjchem-1439 39 26 50 50 NUM eurjchem-1439 39 27 ml ml NOUN eurjchem-1439 39 28 measuring measure VERB eurjchem-1439 39 29 flask flask NOUN eurjchem-1439 39 30 . . PUNCT eurjchem-1439 40 1 the the DET eurjchem-1439 40 2 drug drug NOUN eurjchem-1439 40 3 was be AUX eurjchem-1439 40 4 dissolved dissolve VERB eurjchem-1439 40 5 by by ADP eurjchem-1439 40 6 aid aid NOUN eurjchem-1439 40 7 of of ADP eurjchem-1439 40 8 a a DET eurjchem-1439 40 9 vortex vortex NOUN eurjchem-1439 40 10 mixer mixer NOUN eurjchem-1439 40 11 in in ADP eurjchem-1439 40 12 20 20 NUM eurjchem-1439 40 13 ml ml NOUN eurjchem-1439 40 14 methanol methanol NOUN eurjchem-1439 40 15 then then ADV eurjchem-1439 40 16 the the DET eurjchem-1439 40 17 volume volume NOUN eurjchem-1439 40 18 was be AUX eurjchem-1439 40 19 completed complete VERB eurjchem-1439 40 20 to to ADP eurjchem-1439 40 21 the the DET eurjchem-1439 40 22 mark mark NOUN eurjchem-1439 40 23 using use VERB eurjchem-1439 40 24 the the DET eurjchem-1439 40 25 same same ADJ eurjchem-1439 40 26 solvent solvent NOUN eurjchem-1439 40 27 . . PUNCT eurjchem-1439 41 1 working work VERB eurjchem-1439 41 2 standard standard ADJ eurjchem-1439 41 3 solution solution NOUN eurjchem-1439 41 4 ( ( PUNCT eurjchem-1439 41 5 100 100 NUM eurjchem-1439 41 6 µg µg ADJ eurjchem-1439 41 7 / / SYM eurjchem-1439 41 8 ml ml NOUN eurjchem-1439 41 9 ) ) PUNCT eurjchem-1439 41 10 was be AUX eurjchem-1439 41 11 prepared prepare VERB eurjchem-1439 41 12 by by ADP eurjchem-1439 41 13 accurate accurate ADJ eurjchem-1439 41 14 dilution dilution NOUN eurjchem-1439 41 15 of of ADP eurjchem-1439 41 16 5 5 NUM eurjchem-1439 41 17 ml ml NOUN eurjchem-1439 41 18 of of ADP eurjchem-1439 41 19 the the DET eurjchem-1439 41 20 stock stock NOUN eurjchem-1439 41 21 standard standard ADJ eurjchem-1439 41 22 solution solution NOUN eurjchem-1439 41 23 ( ( PUNCT eurjchem-1439 41 24 1 1 NUM eurjchem-1439 41 25 mg mg NUM eurjchem-1439 41 26 / / SYM eurjchem-1439 41 27 ml ml NOUN eurjchem-1439 41 28 ) ) PUNCT eurjchem-1439 41 29 into into ADP eurjchem-1439 41 30 50 50 NUM eurjchem-1439 41 31 ml ml NOUN eurjchem-1439 41 32 measuring measure VERB eurjchem-1439 41 33 flask flask NOUN eurjchem-1439 41 34 using use VERB eurjchem-1439 41 35 methanol methanol NOUN eurjchem-1439 41 36 as as ADP eurjchem-1439 41 37 a a DET eurjchem-1439 41 38 diluting dilute VERB eurjchem-1439 41 39 solvent solvent NOUN eurjchem-1439 41 40 . . PUNCT eurjchem-1439 42 1 2.5 2.5 NUM eurjchem-1439 42 2 . . PUNCT eurjchem-1439 43 1 method method PROPN eurjchem-1439 43 2 validation validation NOUN eurjchem-1439 43 3 the the DET eurjchem-1439 43 4 developed develop VERB eurjchem-1439 43 5 analytical analytical ADJ eurjchem-1439 43 6 methods method NOUN eurjchem-1439 43 7 were be AUX eurjchem-1439 43 8 fully fully ADV eurjchem-1439 43 9 validated validate VERB eurjchem-1439 43 10 according accord VERB eurjchem-1439 43 11 to to ADP eurjchem-1439 43 12 ich‐q2b ich‐q2b PRON eurjchem-1439 43 13 guidelines guideline NOUN eurjchem-1439 44 1 [ [ X eurjchem-1439 44 2 8 8 NUM eurjchem-1439 44 3 ] ] PUNCT eurjchem-1439 44 4 . . PUNCT eurjchem-1439 45 1 2.5.1 2.5.1 NUM eurjchem-1439 45 2 . . PUNCT eurjchem-1439 46 1 linearity linearity PROPN eurjchem-1439 46 2 2.5.1.1 2.5.1.1 NUM eurjchem-1439 46 3 . . PUNCT eurjchem-1439 47 1 hplc‐method hplc‐method NOUN eurjchem-1439 47 2 aliquots aliquot NOUN eurjchem-1439 47 3 ( ( PUNCT eurjchem-1439 47 4 0.2‐6.0 0.2‐6.0 NUM eurjchem-1439 47 5 ml ml NOUN eurjchem-1439 47 6 ) ) PUNCT eurjchem-1439 47 7 of of ADP eurjchem-1439 47 8 sfv sfv PROPN eurjchem-1439 47 9 working working PROPN eurjchem-1439 47 10 standard standard ADJ eurjchem-1439 47 11 solution solution NOUN eurjchem-1439 47 12 ( ( PUNCT eurjchem-1439 47 13 100 100 NUM eurjchem-1439 47 14 µg µg ADJ eurjchem-1439 47 15 / / SYM eurjchem-1439 47 16 ml ml NOUN eurjchem-1439 47 17 ) ) PUNCT eurjchem-1439 47 18 were be AUX eurjchem-1439 47 19 accurately accurately ADV eurjchem-1439 47 20 and and CCONJ eurjchem-1439 47 21 separately separately ADV eurjchem-1439 47 22 transferred transfer VERB eurjchem-1439 47 23 into into ADP eurjchem-1439 47 24 a a DET eurjchem-1439 47 25 series series NOUN eurjchem-1439 47 26 of of ADP eurjchem-1439 47 27 10 10 NUM eurjchem-1439 47 28 ml ml NOUN eurjchem-1439 47 29 measuring measure VERB eurjchem-1439 47 30 flasks flask NOUN eurjchem-1439 47 31 , , PUNCT eurjchem-1439 47 32 the the DET eurjchem-1439 47 33 volume volume NOUN eurjchem-1439 47 34 was be AUX eurjchem-1439 47 35 then then ADV eurjchem-1439 47 36 completed complete VERB eurjchem-1439 47 37 with with ADP eurjchem-1439 47 38 methanol methanol NOUN eurjchem-1439 47 39 to to PART eurjchem-1439 47 40 obtain obtain VERB eurjchem-1439 47 41 a a DET eurjchem-1439 47 42 concentration concentration NOUN eurjchem-1439 47 43 range range NOUN eurjchem-1439 47 44 of of ADP eurjchem-1439 47 45 2‐ 2‐ NUM eurjchem-1439 47 46 60 60 NUM eurjchem-1439 47 47 µg µg NOUN eurjchem-1439 47 48 / / SYM eurjchem-1439 47 49 ml ml NOUN eurjchem-1439 47 50 . . PUNCT eurjchem-1439 48 1 the the DET eurjchem-1439 48 2 prepared prepare VERB eurjchem-1439 48 3 samples sample NOUN eurjchem-1439 48 4 were be AUX eurjchem-1439 48 5 filtered filter VERB eurjchem-1439 48 6 through through ADP eurjchem-1439 48 7 a a DET eurjchem-1439 48 8 0.45 0.45 NUM eurjchem-1439 48 9 µm µm ADP eurjchem-1439 48 10 membrane membrane NOUN eurjchem-1439 48 11 filter filter NOUN eurjchem-1439 48 12 then then ADV eurjchem-1439 48 13 injection injection NOUN eurjchem-1439 48 14 was be AUX eurjchem-1439 48 15 carried carry VERB eurjchem-1439 48 16 out out ADP eurjchem-1439 48 17 by by ADP eurjchem-1439 48 18 the the DET eurjchem-1439 48 19 aid aid NOUN eurjchem-1439 48 20 of of ADP eurjchem-1439 48 21 a a DET eurjchem-1439 48 22 50 50 NUM eurjchem-1439 48 23 µl µl ADP eurjchem-1439 48 24 hamilton hamilton PROPN eurjchem-1439 48 25 ® ® PROPN eurjchem-1439 48 26 syringe syringe NOUN eurjchem-1439 48 27 . . PUNCT eurjchem-1439 49 1 conditioning conditioning NOUN eurjchem-1439 49 2 and and CCONJ eurjchem-1439 49 3 pre‐washing pre‐washe VERB eurjchem-1439 49 4 of of ADP eurjchem-1439 49 5 the the DET eurjchem-1439 49 6 stationary stationary ADJ eurjchem-1439 49 7 phase phase NOUN eurjchem-1439 49 8 was be AUX eurjchem-1439 49 9 performed perform VERB eurjchem-1439 49 10 by by ADP eurjchem-1439 49 11 passing pass VERB eurjchem-1439 49 12 about about ADV eurjchem-1439 49 13 60‐70 60‐70 NUM eurjchem-1439 49 14 ml ml NOUN eurjchem-1439 49 15 of of ADP eurjchem-1439 49 16 the the DET eurjchem-1439 49 17 mobile mobile ADJ eurjchem-1439 49 18 phase phase NOUN eurjchem-1439 49 19 . . PUNCT eurjchem-1439 50 1 samples sample NOUN eurjchem-1439 50 2 were be AUX eurjchem-1439 50 3 then then ADV eurjchem-1439 50 4 chromatographed chromatographe VERB eurjchem-1439 50 5 using use VERB eurjchem-1439 50 6 hypersiltm hypersiltm PROPN eurjchem-1439 50 7 ods ods PROPN eurjchem-1439 50 8 c18 c18 NOUN eurjchem-1439 50 9 column column NOUN eurjchem-1439 50 10 ( ( PUNCT eurjchem-1439 50 11 250×4.6 250×4.6 NOUN eurjchem-1439 50 12 mm mm NOUN eurjchem-1439 50 13 , , PUNCT eurjchem-1439 50 14 5 5 NUM eurjchem-1439 50 15 µm µm NOUN eurjchem-1439 50 16 ) ) PUNCT eurjchem-1439 50 17 as as ADP eurjchem-1439 50 18 a a DET eurjchem-1439 50 19 stationary stationary ADJ eurjchem-1439 50 20 phase phase NOUN eurjchem-1439 50 21 . . PUNCT eurjchem-1439 51 1 the the DET eurjchem-1439 51 2 mobile mobile ADJ eurjchem-1439 51 3 phase phase NOUN eurjchem-1439 51 4 was be AUX eurjchem-1439 51 5 methanol methanol NOUN eurjchem-1439 51 6 : : PUNCT eurjchem-1439 51 7 acetonitrile acetonitrile NOUN eurjchem-1439 51 8 ( ( PUNCT eurjchem-1439 51 9 90:10 90:10 NUM eurjchem-1439 51 10 , , PUNCT eurjchem-1439 51 11 v v NOUN eurjchem-1439 51 12 : : PUNCT eurjchem-1439 51 13 v v NOUN eurjchem-1439 51 14 ) ) PUNCT eurjchem-1439 51 15 . . PUNCT eurjchem-1439 52 1 it it PRON eurjchem-1439 52 2 was be AUX eurjchem-1439 52 3 pumped pump VERB eurjchem-1439 52 4 using use VERB eurjchem-1439 52 5 isocratic isocratic ADJ eurjchem-1439 52 6 mode mode NOUN eurjchem-1439 52 7 with with ADP eurjchem-1439 52 8 flow flow NOUN eurjchem-1439 52 9 rate rate NOUN eurjchem-1439 52 10 of of ADP eurjchem-1439 52 11 1 1 NUM eurjchem-1439 52 12 ml ml NOUN eurjchem-1439 52 13 / / SYM eurjchem-1439 52 14 min min NOUN eurjchem-1439 52 15 and and CCONJ eurjchem-1439 52 16 uv uv NOUN eurjchem-1439 52 17 detection detection NOUN eurjchem-1439 52 18 at at ADP eurjchem-1439 52 19 260 260 NUM eurjchem-1439 52 20 nm nm NOUN eurjchem-1439 52 21 . . PUNCT eurjchem-1439 53 1 peak peak PROPN eurjchem-1439 53 2 area area NOUN eurjchem-1439 53 3 ratios ratio NOUN eurjchem-1439 53 4 were be AUX eurjchem-1439 53 5 plotted plot VERB eurjchem-1439 53 6 against against ADP eurjchem-1439 53 7 concentration concentration NOUN eurjchem-1439 53 8 to to PART eurjchem-1439 53 9 obtain obtain VERB eurjchem-1439 53 10 the the DET eurjchem-1439 53 11 calibration calibration NOUN eurjchem-1439 53 12 graph graph NOUN eurjchem-1439 53 13 then then ADV eurjchem-1439 53 14 the the DET eurjchem-1439 53 15 regression regression NOUN eurjchem-1439 53 16 equation equation NOUN eurjchem-1439 53 17 was be AUX eurjchem-1439 53 18 computed compute VERB eurjchem-1439 53 19 . . PUNCT eurjchem-1439 54 1 2.5.1.2 2.5.1.2 X eurjchem-1439 54 2 . . PUNCT eurjchem-1439 54 3 uv‐spectrophotometric uv‐spectrophotometric ADJ eurjchem-1439 54 4 method method NOUN eurjchem-1439 54 5 aliquots aliquot NOUN eurjchem-1439 54 6 ( ( PUNCT eurjchem-1439 54 7 0.5‐4.0 0.5‐4.0 NUM eurjchem-1439 54 8 ml ml NOUN eurjchem-1439 54 9 ) ) PUNCT eurjchem-1439 54 10 of of ADP eurjchem-1439 54 11 sfv sfv PROPN eurjchem-1439 54 12 working working PROPN eurjchem-1439 54 13 standard standard ADJ eurjchem-1439 54 14 solution solution NOUN eurjchem-1439 54 15 ( ( PUNCT eurjchem-1439 54 16 100 100 NUM eurjchem-1439 54 17 µg µg ADJ eurjchem-1439 54 18 / / SYM eurjchem-1439 54 19 ml ml NOUN eurjchem-1439 54 20 ) ) PUNCT eurjchem-1439 54 21 were be AUX eurjchem-1439 54 22 transferred transfer VERB eurjchem-1439 54 23 , , PUNCT eurjchem-1439 54 24 accurately accurately ADV eurjchem-1439 54 25 and and CCONJ eurjchem-1439 54 26 separately separately ADV eurjchem-1439 54 27 , , PUNCT eurjchem-1439 54 28 into into ADP eurjchem-1439 54 29 a a DET eurjchem-1439 54 30 series series NOUN eurjchem-1439 54 31 of of ADP eurjchem-1439 54 32 10 10 NUM eurjchem-1439 54 33 ml ml NOUN eurjchem-1439 54 34 measuring measure VERB eurjchem-1439 54 35 flasks flask NOUN eurjchem-1439 54 36 then then ADV eurjchem-1439 54 37 the the DET eurjchem-1439 54 38 volume volume NOUN eurjchem-1439 54 39 was be AUX eurjchem-1439 54 40 completed complete VERB eurjchem-1439 54 41 with with ADP eurjchem-1439 54 42 methanol methanol NOUN eurjchem-1439 54 43 to to PART eurjchem-1439 54 44 obtain obtain VERB eurjchem-1439 54 45 a a DET eurjchem-1439 54 46 concentration concentration NOUN eurjchem-1439 54 47 range range NOUN eurjchem-1439 54 48 of of ADP eurjchem-1439 54 49 5‐ 5‐ NUM eurjchem-1439 54 50 40 40 NUM eurjchem-1439 54 51 µg µg NOUN eurjchem-1439 54 52 / / SYM eurjchem-1439 54 53 ml ml NOUN eurjchem-1439 54 54 . . PUNCT eurjchem-1439 55 1 the the DET eurjchem-1439 55 2 absorption absorption NOUN eurjchem-1439 55 3 spectrum spectrum NOUN eurjchem-1439 55 4 of of ADP eurjchem-1439 55 5 each each DET eurjchem-1439 55 6 solution solution NOUN eurjchem-1439 55 7 was be AUX eurjchem-1439 55 8 scanned scan VERB eurjchem-1439 55 9 using use VERB eurjchem-1439 55 10 methanol methanol NOUN eurjchem-1439 55 11 as as ADP eurjchem-1439 55 12 a a DET eurjchem-1439 55 13 blank blank NOUN eurjchem-1439 55 14 . . PUNCT eurjchem-1439 56 1 absorbance absorbance NOUN eurjchem-1439 56 2 at at ADP eurjchem-1439 56 3 max max NOUN eurjchem-1439 56 4 = = SYM eurjchem-1439 56 5 260 260 NUM eurjchem-1439 56 6 nm nm NOUN eurjchem-1439 56 7 was be AUX eurjchem-1439 56 8 recorded record VERB eurjchem-1439 56 9 for for ADP eurjchem-1439 56 10 each each DET eurjchem-1439 56 11 concentration concentration NOUN eurjchem-1439 56 12 . . PUNCT eurjchem-1439 57 1 the the DET eurjchem-1439 57 2 calibration calibration NOUN eurjchem-1439 57 3 graph graph NOUN eurjchem-1439 57 4 was be AUX eurjchem-1439 57 5 plotted plot VERB eurjchem-1439 57 6 between between ADP eurjchem-1439 57 7 absorbance absorbance NOUN eurjchem-1439 57 8 and and CCONJ eurjchem-1439 57 9 concentration concentration NOUN eurjchem-1439 57 10 then then ADV eurjchem-1439 57 11 the the DET eurjchem-1439 57 12 regression regression NOUN eurjchem-1439 57 13 equation equation NOUN eurjchem-1439 57 14 was be AUX eurjchem-1439 57 15 computed compute VERB eurjchem-1439 57 16 . . PUNCT eurjchem-1439 58 1 2.5.2 2.5.2 NUM eurjchem-1439 58 2 . . PUNCT eurjchem-1439 58 3 accuracy accuracy NOUN eurjchem-1439 58 4 accuracy accuracy NOUN eurjchem-1439 58 5 can can AUX eurjchem-1439 58 6 be be AUX eurjchem-1439 58 7 defined define VERB eurjchem-1439 58 8 as as ADP eurjchem-1439 58 9 the the DET eurjchem-1439 58 10 percent percent NOUN eurjchem-1439 58 11 of of ADP eurjchem-1439 58 12 the the DET eurjchem-1439 58 13 recovered recovered ADJ eurjchem-1439 58 14 analyte analyte NOUN eurjchem-1439 58 15 from from ADP eurjchem-1439 58 16 a a DET eurjchem-1439 58 17 known know VERB eurjchem-1439 58 18 added add VERB eurjchem-1439 58 19 quantity quantity NOUN eurjchem-1439 58 20 [ [ X eurjchem-1439 58 21 9 9 NUM eurjchem-1439 58 22 ] ] PUNCT eurjchem-1439 58 23 . . PUNCT eurjchem-1439 59 1 data datum NOUN eurjchem-1439 59 2 from from ADP eurjchem-1439 59 3 nine nine NUM eurjchem-1439 59 4 samples sample NOUN eurjchem-1439 59 5 representing represent VERB eurjchem-1439 59 6 three three NUM eurjchem-1439 59 7 concentration concentration NOUN eurjchem-1439 59 8 levels level NOUN eurjchem-1439 59 9 covering cover VERB eurjchem-1439 59 10 the the DET eurjchem-1439 59 11 obtained obtain VERB eurjchem-1439 59 12 linearity linearity NOUN eurjchem-1439 59 13 range range NOUN eurjchem-1439 59 14 ( ( PUNCT eurjchem-1439 59 15 10 10 NUM eurjchem-1439 59 16 , , PUNCT eurjchem-1439 59 17 30 30 NUM eurjchem-1439 59 18 and and CCONJ eurjchem-1439 59 19 50 50 NUM eurjchem-1439 59 20 µg µg NOUN eurjchem-1439 59 21 / / SYM eurjchem-1439 59 22 ml ml NOUN eurjchem-1439 59 23 for for ADP eurjchem-1439 59 24 the the DET eurjchem-1439 59 25 hplc hplc NOUN eurjchem-1439 59 26 method method NOUN eurjchem-1439 59 27 and and CCONJ eurjchem-1439 59 28 10 10 NUM eurjchem-1439 59 29 , , PUNCT eurjchem-1439 59 30 20 20 NUM eurjchem-1439 59 31 and and CCONJ eurjchem-1439 59 32 30 30 NUM eurjchem-1439 59 33 µg µg NOUN eurjchem-1439 59 34 / / SYM eurjchem-1439 59 35 ml ml NOUN eurjchem-1439 59 36 for for ADP eurjchem-1439 59 37 the the DET eurjchem-1439 59 38 uv‐spectrophoto‐ uv‐spectrophoto‐ PROPN eurjchem-1439 59 39 metric metric ADJ eurjchem-1439 59 40 method method NOUN eurjchem-1439 59 41 ) ) PUNCT eurjchem-1439 59 42 was be AUX eurjchem-1439 59 43 used use VERB eurjchem-1439 59 44 to to PART eurjchem-1439 59 45 assess assess VERB eurjchem-1439 59 46 accuracy accuracy NOUN eurjchem-1439 59 47 of of ADP eurjchem-1439 59 48 both both DET eurjchem-1439 59 49 methods method NOUN eurjchem-1439 59 50 , , PUNCT eurjchem-1439 59 51 so so ADV eurjchem-1439 59 52 the the DET eurjchem-1439 59 53 previously previously ADV eurjchem-1439 59 54 mentioned mention VERB eurjchem-1439 59 55 procedures procedure NOUN eurjchem-1439 59 56 under under ADP eurjchem-1439 59 57 linearity linearity NOUN eurjchem-1439 59 58 were be AUX eurjchem-1439 59 59 carried carry VERB eurjchem-1439 59 60 out out ADP eurjchem-1439 59 61 then then ADV eurjchem-1439 59 62 the the DET eurjchem-1439 59 63 concentration concentration NOUN eurjchem-1439 59 64 of of ADP eurjchem-1439 59 65 sfv sfv NOUN eurjchem-1439 59 66 samples sample NOUN eurjchem-1439 59 67 was be AUX eurjchem-1439 59 68 calculated calculate VERB eurjchem-1439 59 69 using use VERB eurjchem-1439 59 70 the the DET eurjchem-1439 59 71 corresponding corresponding ADJ eurjchem-1439 59 72 regression regression NOUN eurjchem-1439 59 73 equation equation NOUN eurjchem-1439 59 74 . . PUNCT eurjchem-1439 60 1 2.5.3 2.5.3 NUM eurjchem-1439 60 2 . . PUNCT eurjchem-1439 60 3 precision precision NOUN eurjchem-1439 60 4 precision precision NOUN eurjchem-1439 60 5 can can AUX eurjchem-1439 60 6 be be AUX eurjchem-1439 60 7 defied defy VERB eurjchem-1439 60 8 as as ADP eurjchem-1439 60 9 the the DET eurjchem-1439 60 10 degree degree NOUN eurjchem-1439 60 11 of of ADP eurjchem-1439 60 12 repeatability repeatability NOUN eurjchem-1439 60 13 under under ADP eurjchem-1439 60 14 normal normal ADJ eurjchem-1439 60 15 operational operational ADJ eurjchem-1439 60 16 conditions condition NOUN eurjchem-1439 60 17 . . PUNCT eurjchem-1439 61 1 the the DET eurjchem-1439 61 2 assay assay PROPN eurjchem-1439 61 3 precision precision NOUN eurjchem-1439 61 4 can can AUX eurjchem-1439 61 5 be be AUX eurjchem-1439 61 6 determined determine VERB eurjchem-1439 61 7 by by ADP eurjchem-1439 61 8 repeatability repeatability NOUN eurjchem-1439 61 9 ( ( PUNCT eurjchem-1439 61 10 intra‐day intra‐day NOUN eurjchem-1439 61 11 ) ) PUNCT eurjchem-1439 61 12 and and CCONJ eurjchem-1439 61 13 intermediate intermediate ADJ eurjchem-1439 61 14 precision precision NOUN eurjchem-1439 61 15 ( ( PUNCT eurjchem-1439 61 16 inter‐day inter‐day NOUN eurjchem-1439 61 17 ) ) PUNCT eurjchem-1439 61 18 then then ADV eurjchem-1439 61 19 reported report VERB eurjchem-1439 61 20 as as ADP eurjchem-1439 61 21 % % NOUN eurjchem-1439 61 22 relative relative ADJ eurjchem-1439 61 23 standard standard ADJ eurjchem-1439 61 24 deviation deviation NOUN eurjchem-1439 61 25 ( ( PUNCT eurjchem-1439 61 26 % % INTJ eurjchem-1439 61 27 r.s.d r.s.d NOUN eurjchem-1439 61 28 . . PUNCT eurjchem-1439 61 29 ) ) PUNCT eurjchem-1439 62 1 , , PUNCT eurjchem-1439 62 2 for for ADP eurjchem-1439 62 3 a a DET eurjchem-1439 62 4 number number NOUN eurjchem-1439 62 5 of of ADP eurjchem-1439 62 6 experiments experiment NOUN eurjchem-1439 62 7 which which PRON eurjchem-1439 62 8 are be AUX eurjchem-1439 62 9 statistically statistically ADV eurjchem-1439 62 10 significant significant ADJ eurjchem-1439 62 11 [ [ X eurjchem-1439 62 12 10 10 NUM eurjchem-1439 62 13 ] ] X eurjchem-1439 62 14 so so SCONJ eurjchem-1439 62 15 three three NUM eurjchem-1439 62 16 concentrations concentration NOUN eurjchem-1439 62 17 of of ADP eurjchem-1439 62 18 sfv sfv PROPN eurjchem-1439 62 19 ( ( PUNCT eurjchem-1439 62 20 10 10 NUM eurjchem-1439 62 21 , , PUNCT eurjchem-1439 62 22 30 30 NUM eurjchem-1439 62 23 and and CCONJ eurjchem-1439 62 24 50 50 NUM eurjchem-1439 62 25 µg µg NOUN eurjchem-1439 62 26 / / SYM eurjchem-1439 62 27 ml ml NOUN eurjchem-1439 62 28 for for ADP eurjchem-1439 62 29 the the DET eurjchem-1439 62 30 hplc hplc NOUN eurjchem-1439 62 31 method method NOUN eurjchem-1439 62 32 and and CCONJ eurjchem-1439 62 33 10 10 NUM eurjchem-1439 62 34 , , PUNCT eurjchem-1439 62 35 20 20 NUM eurjchem-1439 62 36 and and CCONJ eurjchem-1439 62 37 30 30 NUM eurjchem-1439 62 38 µg µg NOUN eurjchem-1439 62 39 / / SYM eurjchem-1439 62 40 ml ml NOUN eurjchem-1439 62 41 for for ADP eurjchem-1439 62 42 the the DET eurjchem-1439 62 43 uv‐spectrophotometric uv‐spectrophotometric ADJ eurjchem-1439 62 44 method method NOUN eurjchem-1439 62 45 ) ) PUNCT eurjchem-1439 62 46 were be AUX eurjchem-1439 62 47 analyzed analyze VERB eurjchem-1439 62 48 three three NUM eurjchem-1439 62 49 times time NOUN eurjchem-1439 62 50 within within ADP eurjchem-1439 62 51 the the DET eurjchem-1439 62 52 same same ADJ eurjchem-1439 62 53 day day NOUN eurjchem-1439 62 54 ( ( PUNCT eurjchem-1439 62 55 intra‐day intra‐day NOUN eurjchem-1439 62 56 ) ) PUNCT eurjchem-1439 62 57 or or CCONJ eurjchem-1439 62 58 on on ADP eurjchem-1439 62 59 three three NUM eurjchem-1439 62 60 successive successive ADJ eurjchem-1439 62 61 days day NOUN eurjchem-1439 62 62 ( ( PUNCT eurjchem-1439 62 63 inter‐day inter‐day NOUN eurjchem-1439 62 64 ) ) PUNCT eurjchem-1439 62 65 then then ADV eurjchem-1439 62 66 the the DET eurjchem-1439 62 67 results result NOUN eurjchem-1439 62 68 were be AUX eurjchem-1439 62 69 documented document VERB eurjchem-1439 62 70 as as ADP eurjchem-1439 62 71 % % NOUN eurjchem-1439 62 72 r.s.d r.s.d NOUN eurjchem-1439 62 73 . . PUNCT eurjchem-1439 63 1 2.5.4 2.5.4 NUM eurjchem-1439 63 2 . . PUNCT eurjchem-1439 64 1 specificity specificity NOUN eurjchem-1439 64 2 specificity specificity NOUN eurjchem-1439 64 3 can can AUX eurjchem-1439 64 4 be be AUX eurjchem-1439 64 5 determined determine VERB eurjchem-1439 64 6 by by ADP eurjchem-1439 64 7 comparing compare VERB eurjchem-1439 64 8 the the DET eurjchem-1439 64 9 hpl hpl PROPN eurjchem-1439 64 10 chromatogram chromatogram PROPN eurjchem-1439 64 11 and and CCONJ eurjchem-1439 64 12 the the DET eurjchem-1439 64 13 uv‐scan uv‐scan NOUN eurjchem-1439 64 14 obtained obtain VERB eurjchem-1439 64 15 for for ADP eurjchem-1439 64 16 a a DET eurjchem-1439 64 17 mixture mixture NOUN eurjchem-1439 64 18 of of ADP eurjchem-1439 64 19 sfv sfv PROPN eurjchem-1439 64 20 and and CCONJ eurjchem-1439 64 21 the the DET eurjchem-1439 64 22 commonly commonly ADV eurjchem-1439 64 23 used use VERB eurjchem-1439 64 24 excipients excipient NOUN eurjchem-1439 64 25 , , PUNCT eurjchem-1439 64 26 with with ADP eurjchem-1439 64 27 those those PRON eurjchem-1439 64 28 obtained obtain VERB eurjchem-1439 64 29 from from ADP eurjchem-1439 64 30 the the DET eurjchem-1439 64 31 blank blank NOUN eurjchem-1439 64 32 ( ( PUNCT eurjchem-1439 64 33 excipients excipient NOUN eurjchem-1439 64 34 solution solution NOUN eurjchem-1439 64 35 in in ADP eurjchem-1439 64 36 methanol methanol NOUN eurjchem-1439 64 37 without without ADP eurjchem-1439 64 38 drug drug NOUN eurjchem-1439 64 39 ) ) PUNCT eurjchem-1439 65 1 [ [ X eurjchem-1439 65 2 10 10 NUM eurjchem-1439 65 3 ] ] PUNCT eurjchem-1439 65 4 . . PUNCT eurjchem-1439 66 1 the the DET eurjchem-1439 66 2 chosen choose VERB eurjchem-1439 66 3 excipients excipient NOUN eurjchem-1439 66 4 were be AUX eurjchem-1439 66 5 microcrystalline microcrystalline NOUN eurjchem-1439 66 6 cellulose cellulose NOUN eurjchem-1439 66 7 , , PUNCT eurjchem-1439 66 8 colloi‐ colloi‐ NOUN eurjchem-1439 67 1 dal dal NOUN eurjchem-1439 67 2 silicon silicon NOUN eurjchem-1439 67 3 dioxide dioxide NOUN eurjchem-1439 67 4 , , PUNCT eurjchem-1439 67 5 mannitol mannitol NOUN eurjchem-1439 67 6 , , PUNCT eurjchem-1439 67 7 croscarmellose croscarmellose ADJ eurjchem-1439 67 8 sodium sodium NOUN eurjchem-1439 67 9 , , PUNCT eurjchem-1439 67 10 and and CCONJ eurjchem-1439 67 11 magnesium magnesium NOUN eurjchem-1439 67 12 stearate stearate NOUN eurjchem-1439 67 13 . . PUNCT eurjchem-1439 68 1 these these DET eurjchem-1439 68 2 inactive inactive ADJ eurjchem-1439 68 3 ingredients ingredient NOUN eurjchem-1439 68 4 were be AUX eurjchem-1439 68 5 used use VERB eurjchem-1439 68 6 in in ADP eurjchem-1439 68 7 the the DET eurjchem-1439 68 8 manufacture manufacture NOUN eurjchem-1439 68 9 of of ADP eurjchem-1439 68 10 sovaldi sovaldi ADJ eurjchem-1439 68 11 ® ® NOUN eurjchem-1439 68 12 400 400 NUM eurjchem-1439 68 13 mg mg NOUN eurjchem-1439 68 14 film film NOUN eurjchem-1439 68 15 coated coat VERB eurjchem-1439 68 16 tablets tablet NOUN eurjchem-1439 68 17 as as SCONJ eurjchem-1439 68 18 mentioned mention VERB eurjchem-1439 68 19 in in ADP eurjchem-1439 68 20 its its PRON eurjchem-1439 68 21 monograph monograph NOUN eurjchem-1439 68 22 . . PUNCT eurjchem-1439 69 1 the the DET eurjchem-1439 69 2 drug drug NOUN eurjchem-1439 69 3 to to PART eurjchem-1439 69 4 excipient excipient ADJ eurjchem-1439 69 5 ratio ratio NOUN eurjchem-1439 69 6 was be AUX eurjchem-1439 69 7 similar similar ADJ eurjchem-1439 69 8 to to ADP eurjchem-1439 69 9 that that PRON eurjchem-1439 69 10 used use VERB eurjchem-1439 69 11 in in ADP eurjchem-1439 69 12 the the DET eurjchem-1439 69 13 market market NOUN eurjchem-1439 69 14 product product NOUN eurjchem-1439 69 15 . . PUNCT eurjchem-1439 70 1 2.5.5 2.5.5 X eurjchem-1439 70 2 . . PUNCT eurjchem-1439 70 3 limits limit NOUN eurjchem-1439 70 4 of of ADP eurjchem-1439 70 5 detection detection NOUN eurjchem-1439 70 6 and and CCONJ eurjchem-1439 70 7 quantification quantification NOUN eurjchem-1439 70 8 limits limit NOUN eurjchem-1439 70 9 of of ADP eurjchem-1439 70 10 detection detection NOUN eurjchem-1439 70 11 ( ( PUNCT eurjchem-1439 70 12 lod lod PROPN eurjchem-1439 70 13 ) ) PUNCT eurjchem-1439 70 14 can can AUX eurjchem-1439 70 15 be be AUX eurjchem-1439 70 16 defined define VERB eurjchem-1439 70 17 as as ADP eurjchem-1439 70 18 the the DET eurjchem-1439 70 19 lowest low ADJ eurjchem-1439 70 20 concentration concentration NOUN eurjchem-1439 70 21 of of ADP eurjchem-1439 70 22 the the DET eurjchem-1439 70 23 analyte analyte NOUN eurjchem-1439 70 24 that that PRON eurjchem-1439 70 25 the the DET eurjchem-1439 70 26 analytical analytical ADJ eurjchem-1439 70 27 method method NOUN eurjchem-1439 70 28 can can AUX eurjchem-1439 70 29 reliably reliably ADV eurjchem-1439 70 30 differentiate differentiate VERB eurjchem-1439 70 31 from from ADP eurjchem-1439 70 32 the the DET eurjchem-1439 70 33 background background NOUN eurjchem-1439 70 34 . . PUNCT eurjchem-1439 71 1 limits limit NOUN eurjchem-1439 71 2 of of ADP eurjchem-1439 71 3 quantify‐ quantify‐ ADJ eurjchem-1439 71 4 cation cation NOUN eurjchem-1439 71 5 ( ( PUNCT eurjchem-1439 71 6 loq loq PROPN eurjchem-1439 71 7 ) ) PUNCT eurjchem-1439 71 8 can can AUX eurjchem-1439 71 9 be be AUX eurjchem-1439 71 10 defined define VERB eurjchem-1439 71 11 as as ADP eurjchem-1439 71 12 the the DET eurjchem-1439 71 13 lowest low ADJ eurjchem-1439 71 14 concentration concentration NOUN eurjchem-1439 71 15 that that PRON eurjchem-1439 71 16 can can AUX eurjchem-1439 71 17 be be AUX eurjchem-1439 71 18 quantified quantify VERB eurjchem-1439 71 19 with with ADP eurjchem-1439 71 20 acceptable acceptable ADJ eurjchem-1439 71 21 accuracy accuracy NOUN eurjchem-1439 71 22 and and CCONJ eurjchem-1439 71 23 precision precision NOUN eurjchem-1439 71 24 [ [ X eurjchem-1439 71 25 10 10 NUM eurjchem-1439 71 26 ] ] PUNCT eurjchem-1439 71 27 . . PUNCT eurjchem-1439 72 1 the the DET eurjchem-1439 72 2 lod lod PROPN eurjchem-1439 72 3 and and CCONJ eurjchem-1439 72 4 loq loq PROPN eurjchem-1439 72 5 were be AUX eurjchem-1439 72 6 calculated calculate VERB eurjchem-1439 72 7 as as ADP eurjchem-1439 72 8 lod lod PROPN eurjchem-1439 72 9 = = SYM eurjchem-1439 72 10 3.3 3.3 NUM eurjchem-1439 72 11 σ σ NOUN eurjchem-1439 72 12 / / SYM eurjchem-1439 72 13 s s PROPN eurjchem-1439 72 14 ( ( PUNCT eurjchem-1439 72 15 1 1 NUM eurjchem-1439 72 16 ) ) PUNCT eurjchem-1439 72 17 loq loq NOUN eurjchem-1439 72 18 = = PROPN eurjchem-1439 72 19 10 10 NUM eurjchem-1439 72 20 σ σ PROPN eurjchem-1439 72 21 / / SYM eurjchem-1439 72 22 s s PROPN eurjchem-1439 72 23 ( ( PUNCT eurjchem-1439 72 24 2 2 NUM eurjchem-1439 72 25 ) ) PUNCT eurjchem-1439 72 26 where where SCONJ eurjchem-1439 72 27 , , PUNCT eurjchem-1439 72 28 σ σ PROPN eurjchem-1439 72 29 is be AUX eurjchem-1439 72 30 the the DET eurjchem-1439 72 31 standard standard ADJ eurjchem-1439 72 32 deviation deviation NOUN eurjchem-1439 72 33 of of ADP eurjchem-1439 72 34 the the DET eurjchem-1439 72 35 lowest low ADJ eurjchem-1439 72 36 standard standard ADJ eurjchem-1439 72 37 level level NOUN eurjchem-1439 72 38 and and CCONJ eurjchem-1439 72 39 s s NOUN eurjchem-1439 72 40 is be AUX eurjchem-1439 72 41 the the DET eurjchem-1439 72 42 slope slope NOUN eurjchem-1439 72 43 of of ADP eurjchem-1439 72 44 the the DET eurjchem-1439 72 45 standard standard ADJ eurjchem-1439 72 46 curve curve NOUN eurjchem-1439 72 47 . . PUNCT eurjchem-1439 73 1 2.5.6 2.5.6 X eurjchem-1439 73 2 . . PUNCT eurjchem-1439 74 1 robustness robustness NOUN eurjchem-1439 74 2 robustness robustness NOUN eurjchem-1439 74 3 was be AUX eurjchem-1439 74 4 assessed assess VERB eurjchem-1439 74 5 by by ADP eurjchem-1439 74 6 evaluating evaluate VERB eurjchem-1439 74 7 the the DET eurjchem-1439 74 8 effect effect NOUN eurjchem-1439 74 9 of of ADP eurjchem-1439 74 10 minute minute ADJ eurjchem-1439 74 11 variations variation NOUN eurjchem-1439 74 12 on on ADP eurjchem-1439 74 13 the the DET eurjchem-1439 74 14 proposed propose VERB eurjchem-1439 74 15 methods method NOUN eurjchem-1439 74 16 . . PUNCT eurjchem-1439 75 1 the the DET eurjchem-1439 75 2 conditions condition NOUN eurjchem-1439 75 3 studied study VERB eurjchem-1439 75 4 , , PUNCT eurjchem-1439 75 5 for for ADP eurjchem-1439 75 6 the the DET eurjchem-1439 75 7 hplc‐method hplc‐method NOUN eurjchem-1439 75 8 , , PUNCT eurjchem-1439 75 9 were be AUX eurjchem-1439 75 10 the the DET eurjchem-1439 75 11 flow flow NOUN eurjchem-1439 75 12 rate rate NOUN eurjchem-1439 75 13 variation variation NOUN eurjchem-1439 75 14 by by ADP eurjchem-1439 75 15 0.1 0.1 NUM eurjchem-1439 75 16 ml ml NOUN eurjchem-1439 75 17 / / SYM eurjchem-1439 75 18 min min NOUN eurjchem-1439 75 19 and and CCONJ eurjchem-1439 75 20 mobile mobile ADJ eurjchem-1439 75 21 phase phase NOUN eurjchem-1439 75 22 composition composition NOUN eurjchem-1439 75 23 with with ADP eurjchem-1439 75 24 respect respect NOUN eurjchem-1439 75 25 to to ADP eurjchem-1439 75 26 methanol methanol NOUN eurjchem-1439 75 27 ±5 ±5 NOUN eurjchem-1439 75 28 ml ml X eurjchem-1439 75 29 per per ADP eurjchem-1439 75 30 each each DET eurjchem-1439 75 31 100 100 NUM eurjchem-1439 75 32 ml ml NOUN eurjchem-1439 75 33 of of ADP eurjchem-1439 75 34 the the DET eurjchem-1439 75 35 mobile mobile ADJ eurjchem-1439 75 36 phase phase NOUN eurjchem-1439 75 37 . . PUNCT eurjchem-1439 76 1 on on ADP eurjchem-1439 76 2 the the DET eurjchem-1439 76 3 other other ADJ eurjchem-1439 76 4 hand hand NOUN eurjchem-1439 76 5 , , PUNCT eurjchem-1439 76 6 for for ADP eurjchem-1439 76 7 the the DET eurjchem-1439 76 8 spectrophotometric spectrophotometric ADJ eurjchem-1439 76 9 method method NOUN eurjchem-1439 76 10 the the DET eurjchem-1439 76 11 solvent solvent NOUN eurjchem-1439 76 12 composition composition NOUN eurjchem-1439 76 13 was be AUX eurjchem-1439 76 14 slightly slightly ADV eurjchem-1439 76 15 changed change VERB eurjchem-1439 76 16 by by ADP eurjchem-1439 76 17 adding add VERB eurjchem-1439 76 18 1 1 NUM eurjchem-1439 76 19 % % NOUN eurjchem-1439 76 20 acetonitrile acetonitrile NOUN eurjchem-1439 76 21 . . PUNCT eurjchem-1439 77 1 sherif sherif PROPN eurjchem-1439 77 2 abdel‐naby abdel‐naby PROPN eurjchem-1439 77 3 abdel‐gawad abdel‐gawad PROPN eurjchem-1439 77 4 / / SYM eurjchem-1439 77 5 european european PROPN eurjchem-1439 77 6 journal journal PROPN eurjchem-1439 77 7 of of ADP eurjchem-1439 77 8 chemistry chemistry NOUN eurjchem-1439 77 9 7 7 NUM eurjchem-1439 77 10 ( ( PUNCT eurjchem-1439 77 11 3 3 NUM eurjchem-1439 77 12 ) ) PUNCT eurjchem-1439 77 13 ( ( PUNCT eurjchem-1439 77 14 2016 2016 NUM eurjchem-1439 77 15 ) ) PUNCT eurjchem-1439 77 16 375‐379 375‐379 PROPN eurjchem-1439 77 17 377 377 NUM eurjchem-1439 77 18 figure figure NOUN eurjchem-1439 77 19 1 1 NUM eurjchem-1439 77 20 . . PUNCT eurjchem-1439 78 1 an an DET eurjchem-1439 78 2 hpl hpl PROPN eurjchem-1439 78 3 chromatogram chromatogram NOUN eurjchem-1439 78 4 of of ADP eurjchem-1439 78 5 20 20 NUM eurjchem-1439 78 6 µg µg NOUN eurjchem-1439 78 7 / / SYM eurjchem-1439 78 8 ml ml NOUN eurjchem-1439 78 9 sofosbuvir sofosbuvir PROPN eurjchem-1439 78 10 . . PUNCT eurjchem-1439 79 1 figure figure VERB eurjchem-1439 79 2 2 2 NUM eurjchem-1439 79 3 . . PUNCT eurjchem-1439 80 1 absorption absorption NOUN eurjchem-1439 80 2 spectrum spectrum NOUN eurjchem-1439 80 3 of of ADP eurjchem-1439 80 4 10 10 NUM eurjchem-1439 80 5 µg µg NOUN eurjchem-1439 80 6 / / SYM eurjchem-1439 80 7 ml ml NOUN eurjchem-1439 80 8 sofosbuvir sofosbuvir PROPN eurjchem-1439 80 9 using use VERB eurjchem-1439 80 10 methanol methanol NOUN eurjchem-1439 80 11 as as ADP eurjchem-1439 80 12 blank blank ADJ eurjchem-1439 80 13 . . PUNCT eurjchem-1439 81 1 2.5.7 2.5.7 X eurjchem-1439 81 2 . . PUNCT eurjchem-1439 81 3 system system NOUN eurjchem-1439 81 4 suitability suitability NOUN eurjchem-1439 81 5 the the DET eurjchem-1439 81 6 system system NOUN eurjchem-1439 81 7 suitability suitability NOUN eurjchem-1439 81 8 for for ADP eurjchem-1439 81 9 the the DET eurjchem-1439 81 10 chromatographic chromatographic ADJ eurjchem-1439 81 11 method method NOUN eurjchem-1439 81 12 was be AUX eurjchem-1439 81 13 evaluated evaluate VERB eurjchem-1439 81 14 according accord VERB eurjchem-1439 81 15 to to ADP eurjchem-1439 81 16 tailing tail VERB eurjchem-1439 81 17 factor factor NOUN eurjchem-1439 81 18 , , PUNCT eurjchem-1439 81 19 number number NOUN eurjchem-1439 81 20 of of ADP eurjchem-1439 81 21 theoretical theoretical ADJ eurjchem-1439 81 22 plates plate NOUN eurjchem-1439 81 23 ( ( PUNCT eurjchem-1439 81 24 n n CCONJ eurjchem-1439 81 25 ) ) PUNCT eurjchem-1439 81 26 and and CCONJ eurjchem-1439 81 27 height height NOUN eurjchem-1439 81 28 equivalent equivalent ADJ eurjchem-1439 81 29 to to ADP eurjchem-1439 81 30 theoretical theoretical ADJ eurjchem-1439 81 31 plates plate NOUN eurjchem-1439 81 32 ( ( PUNCT eurjchem-1439 81 33 hetp hetp NOUN eurjchem-1439 81 34 ) ) PUNCT eurjchem-1439 81 35 . . PUNCT eurjchem-1439 82 1 2.6 2.6 NUM eurjchem-1439 82 2 . . PUNCT eurjchem-1439 82 3 analysis analysis NOUN eurjchem-1439 82 4 of of ADP eurjchem-1439 82 5 pharmaceutical pharmaceutical ADJ eurjchem-1439 82 6 formulation formulation NOUN eurjchem-1439 82 7 ten ten NUM eurjchem-1439 82 8 sovaldi sovaldi NOUN eurjchem-1439 82 9 ® ® NOUN eurjchem-1439 82 10 400 400 NUM eurjchem-1439 82 11 mg mg NOUN eurjchem-1439 82 12 film film NOUN eurjchem-1439 82 13 coated coat VERB eurjchem-1439 82 14 tablets tablet NOUN eurjchem-1439 82 15 were be AUX eurjchem-1439 82 16 weighed weigh VERB eurjchem-1439 82 17 to to PART eurjchem-1439 82 18 find find VERB eurjchem-1439 82 19 the the DET eurjchem-1439 82 20 average average ADJ eurjchem-1439 82 21 weight weight NOUN eurjchem-1439 82 22 of of ADP eurjchem-1439 82 23 a a DET eurjchem-1439 82 24 tablet tablet NOUN eurjchem-1439 82 25 then then ADV eurjchem-1439 82 26 crushed crush VERB eurjchem-1439 82 27 , , PUNCT eurjchem-1439 82 28 finely finely ADV eurjchem-1439 82 29 powdered powdered ADJ eurjchem-1439 82 30 and and CCONJ eurjchem-1439 82 31 mixed mixed ADJ eurjchem-1439 82 32 well well ADV eurjchem-1439 82 33 . . PUNCT eurjchem-1439 83 1 tablet tablet NOUN eurjchem-1439 83 2 powder powder NOUN eurjchem-1439 83 3 equivalent equivalent ADJ eurjchem-1439 83 4 to to ADP eurjchem-1439 83 5 10 10 NUM eurjchem-1439 83 6 mg mg NOUN eurjchem-1439 83 7 of of ADP eurjchem-1439 83 8 sfv sfv PROPN eurjchem-1439 83 9 was be AUX eurjchem-1439 83 10 transferred transfer VERB eurjchem-1439 83 11 to to ADP eurjchem-1439 83 12 a a DET eurjchem-1439 83 13 beaker beaker NOUN eurjchem-1439 83 14 of of ADP eurjchem-1439 83 15 250 250 NUM eurjchem-1439 83 16 ml ml NOUN eurjchem-1439 83 17 capacity capacity NOUN eurjchem-1439 83 18 then then ADV eurjchem-1439 83 19 ; ; PUNCT eurjchem-1439 83 20 a a DET eurjchem-1439 83 21 suitable suitable ADJ eurjchem-1439 83 22 volume volume NOUN eurjchem-1439 83 23 of of ADP eurjchem-1439 83 24 methanol methanol NOUN eurjchem-1439 83 25 ( ( PUNCT eurjchem-1439 83 26 40 40 NUM eurjchem-1439 83 27 ml ml NOUN eurjchem-1439 83 28 ) ) PUNCT eurjchem-1439 83 29 was be AUX eurjchem-1439 83 30 added add VERB eurjchem-1439 83 31 and and CCONJ eurjchem-1439 83 32 stirred stir VERB eurjchem-1439 83 33 for for ADP eurjchem-1439 83 34 about about ADV eurjchem-1439 83 35 20 20 NUM eurjchem-1439 83 36 minutes minute NOUN eurjchem-1439 83 37 . . PUNCT eurjchem-1439 84 1 filtration filtration NOUN eurjchem-1439 84 2 was be AUX eurjchem-1439 84 3 carried carry VERB eurjchem-1439 84 4 out out ADP eurjchem-1439 84 5 into into ADP eurjchem-1439 84 6 100 100 NUM eurjchem-1439 84 7 ml ml NOUN eurjchem-1439 84 8 measuring measure VERB eurjchem-1439 84 9 flask flask NOUN eurjchem-1439 84 10 . . PUNCT eurjchem-1439 85 1 the the DET eurjchem-1439 85 2 residue residue NOUN eurjchem-1439 85 3 was be AUX eurjchem-1439 85 4 washed wash VERB eurjchem-1439 85 5 with with ADP eurjchem-1439 85 6 about about ADV eurjchem-1439 85 7 20 20 NUM eurjchem-1439 85 8 ml ml NOUN eurjchem-1439 85 9 methanol methanol NOUN eurjchem-1439 85 10 ( ( PUNCT eurjchem-1439 85 11 twice twice ADV eurjchem-1439 85 12 ) ) PUNCT eurjchem-1439 85 13 , , PUNCT eurjchem-1439 85 14 and and CCONJ eurjchem-1439 85 15 then then ADV eurjchem-1439 85 16 the the DET eurjchem-1439 85 17 volume volume NOUN eurjchem-1439 85 18 was be AUX eurjchem-1439 85 19 completed complete VERB eurjchem-1439 85 20 to to ADP eurjchem-1439 85 21 the the DET eurjchem-1439 85 22 mark mark NOUN eurjchem-1439 85 23 with with ADP eurjchem-1439 85 24 methanol methanol NOUN eurjchem-1439 85 25 and and CCONJ eurjchem-1439 85 26 mixed mixed ADJ eurjchem-1439 85 27 well well ADV eurjchem-1439 85 28 . . PUNCT eurjchem-1439 86 1 the the DET eurjchem-1439 86 2 general general ADJ eurjchem-1439 86 3 procedures procedure NOUN eurjchem-1439 86 4 were be AUX eurjchem-1439 86 5 followed follow VERB eurjchem-1439 86 6 as as SCONJ eurjchem-1439 86 7 mentioned mention VERB eurjchem-1439 86 8 under under ADP eurjchem-1439 86 9 linearity linearity NOUN eurjchem-1439 86 10 for for ADP eurjchem-1439 86 11 both both DET eurjchem-1439 86 12 techniques technique NOUN eurjchem-1439 86 13 then then ADV eurjchem-1439 86 14 the the DET eurjchem-1439 86 15 concentration concentration NOUN eurjchem-1439 86 16 of of ADP eurjchem-1439 86 17 sfv sfv PROPN eurjchem-1439 86 18 was be AUX eurjchem-1439 86 19 obtained obtain VERB eurjchem-1439 86 20 from from ADP eurjchem-1439 86 21 the the DET eurjchem-1439 86 22 corresponding corresponding ADJ eurjchem-1439 86 23 regression regression NOUN eurjchem-1439 86 24 equation equation NOUN eurjchem-1439 86 25 . . PUNCT eurjchem-1439 87 1 moreover moreover ADV eurjchem-1439 87 2 , , PUNCT eurjchem-1439 87 3 standard standard ADJ eurjchem-1439 87 4 addition addition NOUN eurjchem-1439 87 5 procedure procedure NOUN eurjchem-1439 87 6 was be AUX eurjchem-1439 87 7 applied apply VERB eurjchem-1439 87 8 by by ADP eurjchem-1439 87 9 spiking spike VERB eurjchem-1439 87 10 different different ADJ eurjchem-1439 87 11 known know VERB eurjchem-1439 87 12 quantities quantity NOUN eurjchem-1439 87 13 of of ADP eurjchem-1439 87 14 pure pure ADJ eurjchem-1439 87 15 sfv sfv PROPN eurjchem-1439 87 16 to to ADP eurjchem-1439 87 17 the the DET eurjchem-1439 87 18 tablet tablet NOUN eurjchem-1439 87 19 formulation formulation NOUN eurjchem-1439 87 20 , , PUNCT eurjchem-1439 87 21 and and CCONJ eurjchem-1439 87 22 then then ADV eurjchem-1439 87 23 the the DET eurjchem-1439 87 24 procedures procedure NOUN eurjchem-1439 87 25 were be AUX eurjchem-1439 87 26 followed follow VERB eurjchem-1439 87 27 as as SCONJ eurjchem-1439 87 28 mentioned mention VERB eurjchem-1439 87 29 before before ADV eurjchem-1439 87 30 . . PUNCT eurjchem-1439 88 1 3 3 X eurjchem-1439 88 2 . . NOUN eurjchem-1439 88 3 results result NOUN eurjchem-1439 88 4 and and CCONJ eurjchem-1439 88 5 discussion discussion NOUN eurjchem-1439 88 6 two two NUM eurjchem-1439 88 7 simple simple ADJ eurjchem-1439 88 8 analytical analytical ADJ eurjchem-1439 88 9 methods method NOUN eurjchem-1439 88 10 were be AUX eurjchem-1439 88 11 developed develop VERB eurjchem-1439 88 12 for for ADP eurjchem-1439 88 13 the the DET eurjchem-1439 88 14 quantification quantification NOUN eurjchem-1439 88 15 of of ADP eurjchem-1439 88 16 sfv sfv PROPN eurjchem-1439 88 17 either either CCONJ eurjchem-1439 88 18 in in ADP eurjchem-1439 88 19 pure pure ADJ eurjchem-1439 88 20 or or CCONJ eurjchem-1439 88 21 tablet tablet NOUN eurjchem-1439 88 22 forms form NOUN eurjchem-1439 88 23 . . PUNCT eurjchem-1439 89 1 these these DET eurjchem-1439 89 2 methods method NOUN eurjchem-1439 89 3 can can AUX eurjchem-1439 89 4 be be AUX eurjchem-1439 89 5 conveniently conveniently ADV eurjchem-1439 89 6 applied apply VERB eurjchem-1439 89 7 for for ADP eurjchem-1439 89 8 the the DET eurjchem-1439 89 9 routine routine ADJ eurjchem-1439 89 10 quality quality NOUN eurjchem-1439 89 11 control control NOUN eurjchem-1439 89 12 analysis analysis NOUN eurjchem-1439 89 13 of of ADP eurjchem-1439 89 14 pharmaceutical pharmaceutical ADJ eurjchem-1439 89 15 dosage dosage NOUN eurjchem-1439 89 16 forms form NOUN eurjchem-1439 89 17 . . PUNCT eurjchem-1439 90 1 both both DET eurjchem-1439 90 2 methods method NOUN eurjchem-1439 90 3 were be AUX eurjchem-1439 90 4 developed develop VERB eurjchem-1439 90 5 and and CCONJ eurjchem-1439 90 6 validated validate VERB eurjchem-1439 90 7 according accord VERB eurjchem-1439 90 8 to to ADP eurjchem-1439 90 9 the the DET eurjchem-1439 90 10 guidelines guideline NOUN eurjchem-1439 90 11 of of ADP eurjchem-1439 90 12 ich‐q2b ich‐q2b NOUN eurjchem-1439 90 13 [ [ X eurjchem-1439 90 14 8 8 NUM eurjchem-1439 90 15 ] ] PUNCT eurjchem-1439 90 16 . . PUNCT eurjchem-1439 91 1 the the DET eurjchem-1439 91 2 first first ADJ eurjchem-1439 91 3 one one NOUN eurjchem-1439 91 4 is be AUX eurjchem-1439 91 5 an an DET eurjchem-1439 91 6 isocratic isocratic NOUN eurjchem-1439 91 7 reversed reverse VERB eurjchem-1439 91 8 phase phase NOUN eurjchem-1439 91 9 high‐ high‐ NOUN eurjchem-1439 91 10 performance performance NOUN eurjchem-1439 91 11 liquid liquid ADJ eurjchem-1439 91 12 chromatographic chromatographic ADJ eurjchem-1439 91 13 ( ( PUNCT eurjchem-1439 91 14 rp‐hplc rp‐hplc NOUN eurjchem-1439 91 15 ) ) PUNCT eurjchem-1439 91 16 method method NOUN eurjchem-1439 91 17 . . PUNCT eurjchem-1439 92 1 the the DET eurjchem-1439 92 2 parameters parameter NOUN eurjchem-1439 92 3 were be AUX eurjchem-1439 92 4 optimized optimize VERB eurjchem-1439 92 5 to to PART eurjchem-1439 92 6 get get VERB eurjchem-1439 92 7 an an DET eurjchem-1439 92 8 optimum optimum ADJ eurjchem-1439 92 9 performance performance NOUN eurjchem-1439 92 10 of of ADP eurjchem-1439 92 11 the the DET eurjchem-1439 92 12 method method NOUN eurjchem-1439 92 13 . . PUNCT eurjchem-1439 93 1 the the DET eurjchem-1439 93 2 mobile mobile ADJ eurjchem-1439 93 3 phase phase NOUN eurjchem-1439 93 4 selection selection NOUN eurjchem-1439 93 5 was be AUX eurjchem-1439 93 6 done do VERB eurjchem-1439 93 7 depending depend VERB eurjchem-1439 93 8 on on ADP eurjchem-1439 93 9 the the DET eurjchem-1439 93 10 polarity polarity NOUN eurjchem-1439 93 11 of of ADP eurjchem-1439 93 12 the the DET eurjchem-1439 93 13 studied study VERB eurjchem-1439 93 14 drug drug NOUN eurjchem-1439 93 15 . . PUNCT eurjchem-1439 94 1 different different ADJ eurjchem-1439 94 2 stationary stationary ADJ eurjchem-1439 94 3 and and CCONJ eurjchem-1439 94 4 mobile mobile ADJ eurjchem-1439 94 5 phases phase NOUN eurjchem-1439 94 6 were be AUX eurjchem-1439 94 7 tried try VERB eurjchem-1439 94 8 to to PART eurjchem-1439 94 9 reach reach VERB eurjchem-1439 94 10 the the DET eurjchem-1439 94 11 best good ADJ eurjchem-1439 94 12 match match NOUN eurjchem-1439 94 13 and and CCONJ eurjchem-1439 94 14 so so ADV eurjchem-1439 94 15 get get VERB eurjchem-1439 94 16 the the DET eurjchem-1439 94 17 best good ADJ eurjchem-1439 94 18 separation separation NOUN eurjchem-1439 94 19 pattern pattern NOUN eurjchem-1439 94 20 of of ADP eurjchem-1439 94 21 sfv sfv PROPN eurjchem-1439 94 22 . . PUNCT eurjchem-1439 95 1 the the DET eurjchem-1439 95 2 best good ADJ eurjchem-1439 95 3 gaussian gaussian ADJ eurjchem-1439 95 4 peak peak NOUN eurjchem-1439 95 5 with with ADP eurjchem-1439 95 6 ideal ideal ADJ eurjchem-1439 95 7 peak peak NOUN eurjchem-1439 95 8 symmetry symmetry NOUN eurjchem-1439 95 9 was be AUX eurjchem-1439 95 10 obtained obtain VERB eurjchem-1439 95 11 on on ADP eurjchem-1439 95 12 using use VERB eurjchem-1439 95 13 hypersiltm hypersiltm PROPN eurjchem-1439 95 14 ods ods PROPN eurjchem-1439 95 15 c18 c18 NOUN eurjchem-1439 95 16 column column NOUN eurjchem-1439 95 17 ( ( PUNCT eurjchem-1439 95 18 150×4.6 150×4.6 NOUN eurjchem-1439 95 19 mm mm NOUN eurjchem-1439 95 20 , , PUNCT eurjchem-1439 95 21 5 5 NUM eurjchem-1439 95 22 µm µm NOUN eurjchem-1439 95 23 ) ) PUNCT eurjchem-1439 95 24 as as ADP eurjchem-1439 95 25 a a DET eurjchem-1439 95 26 stationary stationary ADJ eurjchem-1439 95 27 phase phase NOUN eurjchem-1439 95 28 and and CCONJ eurjchem-1439 95 29 methanol methanol NOUN eurjchem-1439 95 30 : : PUNCT eurjchem-1439 95 31 acetonitrile acetonitrile NOUN eurjchem-1439 95 32 ( ( PUNCT eurjchem-1439 95 33 90:10 90:10 NUM eurjchem-1439 95 34 , , PUNCT eurjchem-1439 95 35 v v NOUN eurjchem-1439 95 36 : : PUNCT eurjchem-1439 95 37 v v NOUN eurjchem-1439 95 38 ) ) PUNCT eurjchem-1439 95 39 as as ADP eurjchem-1439 95 40 a a DET eurjchem-1439 95 41 mobile mobile ADJ eurjchem-1439 95 42 phase phase NOUN eurjchem-1439 95 43 . . PUNCT eurjchem-1439 96 1 the the DET eurjchem-1439 96 2 mobile mobile ADJ eurjchem-1439 96 3 phase phase NOUN eurjchem-1439 96 4 flow flow NOUN eurjchem-1439 96 5 was be AUX eurjchem-1439 96 6 operated operate VERB eurjchem-1439 96 7 using use VERB eurjchem-1439 96 8 the the DET eurjchem-1439 96 9 isocratic isocratic ADJ eurjchem-1439 96 10 mode mode NOUN eurjchem-1439 96 11 with with ADP eurjchem-1439 96 12 a a DET eurjchem-1439 96 13 rate rate NOUN eurjchem-1439 96 14 of of ADP eurjchem-1439 96 15 1 1 NUM eurjchem-1439 96 16 ml ml NOUN eurjchem-1439 96 17 / / SYM eurjchem-1439 96 18 min min NOUN eurjchem-1439 96 19 and and CCONJ eurjchem-1439 96 20 uv uv NOUN eurjchem-1439 96 21 detection detection NOUN eurjchem-1439 96 22 at at ADP eurjchem-1439 96 23 260 260 NUM eurjchem-1439 96 24 nm nm NOUN eurjchem-1439 96 25 . . PUNCT eurjchem-1439 97 1 by by ADP eurjchem-1439 97 2 adopting adopt VERB eurjchem-1439 97 3 the the DET eurjchem-1439 97 4 described describe VERB eurjchem-1439 97 5 chromatographic chromatographic ADJ eurjchem-1439 97 6 conditions condition NOUN eurjchem-1439 97 7 , , PUNCT eurjchem-1439 97 8 sfv sfv PROPN eurjchem-1439 97 9 was be AUX eurjchem-1439 97 10 well well ADV eurjchem-1439 97 11 separated separate VERB eurjchem-1439 97 12 with with ADP eurjchem-1439 97 13 average average ADJ eurjchem-1439 97 14 retention retention NOUN eurjchem-1439 97 15 time time NOUN eurjchem-1439 97 16 of of ADP eurjchem-1439 97 17 1.99±0.05 1.99±0.05 PROPN eurjchem-1439 97 18 min min PROPN eurjchem-1439 97 19 as as SCONJ eurjchem-1439 97 20 shown show VERB eurjchem-1439 97 21 in in ADP eurjchem-1439 97 22 figure figure NOUN eurjchem-1439 97 23 1 1 NUM eurjchem-1439 97 24 . . PUNCT eurjchem-1439 98 1 on on ADP eurjchem-1439 98 2 the the DET eurjchem-1439 98 3 other other ADJ eurjchem-1439 98 4 hand hand NOUN eurjchem-1439 98 5 , , PUNCT eurjchem-1439 98 6 the the DET eurjchem-1439 98 7 second second ADJ eurjchem-1439 98 8 method method NOUN eurjchem-1439 98 9 was be AUX eurjchem-1439 98 10 based base VERB eurjchem-1439 98 11 on on ADP eurjchem-1439 98 12 the the DET eurjchem-1439 98 13 absorption absorption NOUN eurjchem-1439 98 14 power power NOUN eurjchem-1439 98 15 of of ADP eurjchem-1439 98 16 sfv sfv PROPN eurjchem-1439 98 17 in in ADP eurjchem-1439 98 18 the the DET eurjchem-1439 98 19 ultra ultra ADJ eurjchem-1439 98 20 violet violet NOUN eurjchem-1439 98 21 ( ( PUNCT eurjchem-1439 98 22 uv uv NOUN eurjchem-1439 98 23 ) ) PUNCT eurjchem-1439 98 24 region region NOUN eurjchem-1439 98 25 . . PUNCT eurjchem-1439 99 1 sfv sfv PROPN eurjchem-1439 99 2 has have VERB eurjchem-1439 99 3 specific specific ADJ eurjchem-1439 99 4 structural structural ADJ eurjchem-1439 99 5 arrangement arrangement NOUN eurjchem-1439 99 6 that that PRON eurjchem-1439 99 7 absorb absorb VERB eurjchem-1439 99 8 uv uv NOUN eurjchem-1439 99 9 rays ray NOUN eurjchem-1439 99 10 at at ADP eurjchem-1439 99 11 a a DET eurjchem-1439 99 12 specific specific ADJ eurjchem-1439 99 13 wavelength wavelength NOUN eurjchem-1439 99 14 ( ( PUNCT eurjchem-1439 99 15 260 260 NUM eurjchem-1439 99 16 nm nm NOUN eurjchem-1439 99 17 ) ) PUNCT eurjchem-1439 99 18 and and CCONJ eurjchem-1439 99 19 this this DET eurjchem-1439 99 20 information information NOUN eurjchem-1439 99 21 was be AUX eurjchem-1439 99 22 successfully successfully ADV eurjchem-1439 99 23 used use VERB eurjchem-1439 99 24 for for ADP eurjchem-1439 99 25 its its PRON eurjchem-1439 99 26 determination determination NOUN eurjchem-1439 99 27 , , PUNCT eurjchem-1439 99 28 figure figure NOUN eurjchem-1439 99 29 2 2 NUM eurjchem-1439 99 30 . . SYM eurjchem-1439 99 31 3.1 3.1 NUM eurjchem-1439 99 32 . . PUNCT eurjchem-1439 100 1 method method NOUN eurjchem-1439 100 2 validation validation NOUN eurjchem-1439 100 3 3.1.1 3.1.1 NUM eurjchem-1439 100 4 . . PUNCT eurjchem-1439 101 1 linearity linearity PROPN eurjchem-1439 101 2 3.1.1.1 3.1.1.1 NUM eurjchem-1439 101 3 . . PUNCT eurjchem-1439 101 4 hplc‐method hplc‐method NOUN eurjchem-1439 101 5 calibration calibration NOUN eurjchem-1439 101 6 standards standard NOUN eurjchem-1439 101 7 at at ADP eurjchem-1439 101 8 seven seven NUM eurjchem-1439 101 9 levels level NOUN eurjchem-1439 101 10 were be AUX eurjchem-1439 101 11 prepared prepare VERB eurjchem-1439 101 12 by by ADP eurjchem-1439 101 13 suitable suitable ADJ eurjchem-1439 101 14 dilution dilution NOUN eurjchem-1439 101 15 of of ADP eurjchem-1439 101 16 the the DET eurjchem-1439 101 17 working work VERB eurjchem-1439 101 18 standard standard ADJ eurjchem-1439 101 19 solution solution NOUN eurjchem-1439 101 20 of of ADP eurjchem-1439 101 21 sfv sfv PROPN eurjchem-1439 101 22 ( ( PUNCT eurjchem-1439 101 23 100 100 NUM eurjchem-1439 101 24 µg µg NOUN eurjchem-1439 101 25 / / SYM eurjchem-1439 101 26 ml ml NOUN eurjchem-1439 101 27 ) ) PUNCT eurjchem-1439 101 28 to to PART eurjchem-1439 101 29 reach reach VERB eurjchem-1439 101 30 concentration concentration NOUN eurjchem-1439 101 31 range range NOUN eurjchem-1439 101 32 of of ADP eurjchem-1439 101 33 2‐60 2‐60 NUM eurjchem-1439 101 34 µg µg ADV eurjchem-1439 101 35 / / SYM eurjchem-1439 101 36 ml ml NOUN eurjchem-1439 101 37 . . PUNCT eurjchem-1439 102 1 each each DET eurjchem-1439 102 2 concentration concentration NOUN eurjchem-1439 102 3 was be AUX eurjchem-1439 102 4 injected inject VERB eurjchem-1439 102 5 in in ADP eurjchem-1439 102 6 triplicate triplicate NOUN eurjchem-1439 102 7 , , PUNCT eurjchem-1439 102 8 and and CCONJ eurjchem-1439 102 9 peak peak NOUN eurjchem-1439 102 10 area area NOUN eurjchem-1439 102 11 ratios ratio NOUN eurjchem-1439 102 12 were be AUX eurjchem-1439 102 13 plotted plot VERB eurjchem-1439 102 14 against against ADP eurjchem-1439 102 15 the the DET eurjchem-1439 102 16 corresponding correspond VERB eurjchem-1439 102 17 concentrations concentration NOUN eurjchem-1439 102 18 to to PART eurjchem-1439 102 19 obtain obtain VERB eurjchem-1439 102 20 the the DET eurjchem-1439 102 21 calibration calibration NOUN eurjchem-1439 102 22 curve curve NOUN eurjchem-1439 102 23 then then ADV eurjchem-1439 102 24 the the DET eurjchem-1439 102 25 regression regression NOUN eurjchem-1439 102 26 equation equation NOUN eurjchem-1439 102 27 was be AUX eurjchem-1439 102 28 computed compute VERB eurjchem-1439 102 29 to to PART eurjchem-1439 102 30 be be AUX eurjchem-1439 102 31 : : PUNCT eurjchem-1439 102 32 p p X eurjchem-1439 102 33 = = PUNCT eurjchem-1439 102 34 0.0337 0.0337 NUM eurjchem-1439 102 35 × × NOUN eurjchem-1439 102 36 c c NOUN eurjchem-1439 102 37 + + NOUN eurjchem-1439 102 38 0.0091 0.0091 NUM eurjchem-1439 102 39 ( ( PUNCT eurjchem-1439 102 40 r2 r2 PROPN eurjchem-1439 102 41 = = PUNCT eurjchem-1439 102 42 0.9996 0.9996 NUM eurjchem-1439 102 43 ) ) PUNCT eurjchem-1439 102 44 ( ( PUNCT eurjchem-1439 102 45 3 3 X eurjchem-1439 102 46 ) ) PUNCT eurjchem-1439 102 47 where where SCONJ eurjchem-1439 102 48 , , PUNCT eurjchem-1439 102 49 p p NOUN eurjchem-1439 102 50 is be AUX eurjchem-1439 102 51 the the DET eurjchem-1439 102 52 peak peak NOUN eurjchem-1439 102 53 area area NOUN eurjchem-1439 102 54 ratio ratio NOUN eurjchem-1439 102 55 and and CCONJ eurjchem-1439 102 56 c c NOUN eurjchem-1439 102 57 is be AUX eurjchem-1439 102 58 the the DET eurjchem-1439 102 59 concentration concentration NOUN eurjchem-1439 102 60 in in ADP eurjchem-1439 102 61 µg µg ADV eurjchem-1439 102 62 / / SYM eurjchem-1439 102 63 ml ml NOUN eurjchem-1439 102 64 . . PUNCT eurjchem-1439 103 1 3.1.1.2 3.1.1.2 NUM eurjchem-1439 103 2 . . NOUN eurjchem-1439 103 3 spectrophotometric spectrophotometric ADJ eurjchem-1439 103 4 method method VERB eurjchem-1439 103 5 five five NUM eurjchem-1439 103 6 concentration concentration NOUN eurjchem-1439 103 7 levels level NOUN eurjchem-1439 103 8 were be AUX eurjchem-1439 103 9 prepared prepare VERB eurjchem-1439 103 10 from from ADP eurjchem-1439 103 11 the the DET eurjchem-1439 103 12 working work VERB eurjchem-1439 103 13 standard standard ADJ eurjchem-1439 103 14 solution solution NOUN eurjchem-1439 103 15 of of ADP eurjchem-1439 103 16 sfv sfv PROPN eurjchem-1439 103 17 ( ( PUNCT eurjchem-1439 103 18 100 100 NUM eurjchem-1439 103 19 µg µg NOUN eurjchem-1439 103 20 / / SYM eurjchem-1439 103 21 ml ml NOUN eurjchem-1439 103 22 ) ) PUNCT eurjchem-1439 103 23 in in ADP eurjchem-1439 103 24 the the DET eurjchem-1439 103 25 range range NOUN eurjchem-1439 103 26 5‐40 5‐40 NUM eurjchem-1439 103 27 µg µg NOUN eurjchem-1439 103 28 / / SYM eurjchem-1439 103 29 ml ml NOUN eurjchem-1439 103 30 . . PUNCT eurjchem-1439 104 1 a a DET eurjchem-1439 104 2 linear linear ADJ eurjchem-1439 104 3 correlation correlation NOUN eurjchem-1439 104 4 was be AUX eurjchem-1439 104 5 obtained obtain VERB eurjchem-1439 104 6 between between ADP eurjchem-1439 104 7 absorbance absorbance NOUN eurjchem-1439 104 8 at at ADP eurjchem-1439 104 9 260 260 NUM eurjchem-1439 104 10 nm nm NOUN eurjchem-1439 104 11 and and CCONJ eurjchem-1439 104 12 the the DET eurjchem-1439 104 13 corresponding corresponding ADJ eurjchem-1439 104 14 concentration concentration NOUN eurjchem-1439 104 15 of of ADP eurjchem-1439 104 16 the the DET eurjchem-1439 104 17 studied study VERB eurjchem-1439 104 18 drug drug NOUN eurjchem-1439 104 19 . . PUNCT eurjchem-1439 105 1 the the DET eurjchem-1439 105 2 following follow VERB eurjchem-1439 105 3 regression regression NOUN eurjchem-1439 105 4 equation equation NOUN eurjchem-1439 105 5 was be AUX eurjchem-1439 105 6 computed compute VERB eurjchem-1439 105 7 to to PART eurjchem-1439 105 8 be be AUX eurjchem-1439 105 9 : : PUNCT eurjchem-1439 105 10 a a DET eurjchem-1439 105 11 = = PUNCT eurjchem-1439 105 12 0.029 0.029 NUM eurjchem-1439 105 13 × × NOUN eurjchem-1439 105 14 c c PROPN eurjchem-1439 105 15 – – PUNCT eurjchem-1439 105 16 0.0073 0.0073 NUM eurjchem-1439 105 17 ( ( PUNCT eurjchem-1439 105 18 r2 r2 PROPN eurjchem-1439 105 19 = = PUNCT eurjchem-1439 105 20 0.9997 0.9997 NUM eurjchem-1439 105 21 ) ) PUNCT eurjchem-1439 105 22 ( ( PUNCT eurjchem-1439 105 23 4 4 X eurjchem-1439 105 24 ) ) PUNCT eurjchem-1439 105 25 378 378 NUM eurjchem-1439 105 26 sherif sherif PROPN eurjchem-1439 105 27 abdel‐naby abdel‐naby PROPN eurjchem-1439 105 28 abdel‐gawad abdel‐gawad PROPN eurjchem-1439 105 29 / / SYM eurjchem-1439 105 30 european european PROPN eurjchem-1439 105 31 journal journal PROPN eurjchem-1439 105 32 of of ADP eurjchem-1439 105 33 chemistry chemistry NOUN eurjchem-1439 105 34 7 7 NUM eurjchem-1439 105 35 ( ( PUNCT eurjchem-1439 105 36 3 3 NUM eurjchem-1439 105 37 ) ) PUNCT eurjchem-1439 105 38 ( ( PUNCT eurjchem-1439 105 39 2016 2016 NUM eurjchem-1439 105 40 ) ) PUNCT eurjchem-1439 105 41 375‐379 375‐379 PROPN eurjchem-1439 105 42 table table NOUN eurjchem-1439 105 43 1 1 NUM eurjchem-1439 105 44 . . PUNCT eurjchem-1439 105 45 method method NOUN eurjchem-1439 105 46 validation validation NOUN eurjchem-1439 105 47 parameters parameter NOUN eurjchem-1439 105 48 for for ADP eurjchem-1439 105 49 determination determination NOUN eurjchem-1439 105 50 of of ADP eurjchem-1439 105 51 sofosbuvir sofosbuvir PROPN eurjchem-1439 105 52 by by ADP eurjchem-1439 105 53 the the DET eurjchem-1439 105 54 adopted adopt VERB eurjchem-1439 105 55 methods method NOUN eurjchem-1439 105 56 . . PUNCT eurjchem-1439 106 1 parameter parameter PROPN eurjchem-1439 106 2 method method PROPN eurjchem-1439 106 3 hplc hplc PROPN eurjchem-1439 106 4 uv uv NOUN eurjchem-1439 106 5 spectrophotometry spectrophotometry NOUN eurjchem-1439 106 6 linearity linearity PROPN eurjchem-1439 106 7 range range NOUN eurjchem-1439 106 8 , , PUNCT eurjchem-1439 106 9 µg µg VERB eurjchem-1439 106 10 / / SYM eurjchem-1439 106 11 ml ml ADP eurjchem-1439 106 12 2‐60 2‐60 NUM eurjchem-1439 106 13 5‐40 5‐40 NUM eurjchem-1439 106 14 slope slope NOUN eurjchem-1439 106 15 0.0337 0.0337 NUM eurjchem-1439 106 16 0.029 0.029 NUM eurjchem-1439 106 17 intercept intercept NOUN eurjchem-1439 106 18 0.0091 0.0091 NUM eurjchem-1439 106 19 ‐0.0073 ‐0.0073 NOUN eurjchem-1439 106 20 r2 r2 NOUN eurjchem-1439 106 21 0.9996 0.9996 NUM eurjchem-1439 106 22 0.9997 0.9997 NUM eurjchem-1439 106 23 accuracy accuracy NOUN eurjchem-1439 106 24 mean±s.d mean±s.d PROPN eurjchem-1439 106 25 . . PUNCT eurjchem-1439 107 1 a a DET eurjchem-1439 107 2 98.94±0.899 98.94±0.899 NUM eurjchem-1439 107 3 100.96±0.745 100.96±0.745 NOUN eurjchem-1439 107 4 variance variance NOUN eurjchem-1439 107 5 0.808 0.808 NUM eurjchem-1439 107 6 0.555 0.555 NUM eurjchem-1439 107 7 % % NOUN eurjchem-1439 107 8 r.s.d r.s.d NOUN eurjchem-1439 107 9 . . PUNCT eurjchem-1439 108 1 0.909 0.909 NUM eurjchem-1439 108 2 0.738 0.738 NUM eurjchem-1439 108 3 precision precision NOUN eurjchem-1439 108 4 intraday intraday NOUN eurjchem-1439 108 5 precision precision NOUN eurjchem-1439 108 6 , , PUNCT eurjchem-1439 108 7 % % INTJ eurjchem-1439 109 1 99.81±1.121 99.81±1.121 NUM eurjchem-1439 110 1 100.94±0.874 100.94±0.874 NUM eurjchem-1439 110 2 interday interday PROPN eurjchem-1439 110 3 precision precision NOUN eurjchem-1439 110 4 , , PUNCT eurjchem-1439 110 5 % % NOUN eurjchem-1439 111 1 98.59±1.412 98.59±1.412 NUM eurjchem-1439 111 2 101.84±1.028 101.84±1.028 ADJ eurjchem-1439 111 3 robustness robustness NOUN eurjchem-1439 111 4 0.657 0.657 NUM eurjchem-1439 111 5 b b NUM eurjchem-1439 111 6 0.691 0.691 NUM eurjchem-1439 111 7 0.574 0.574 NUM eurjchem-1439 111 8 c c NOUN eurjchem-1439 111 9 lod lod PROPN eurjchem-1439 111 10 , , PUNCT eurjchem-1439 111 11 µg µg ADJ eurjchem-1439 111 12 / / SYM eurjchem-1439 111 13 ml ml ADP eurjchem-1439 111 14 0.25 0.25 NUM eurjchem-1439 111 15 1.50 1.50 NUM eurjchem-1439 111 16 loq loq PROPN eurjchem-1439 111 17 , , PUNCT eurjchem-1439 111 18 µg µg NOUN eurjchem-1439 111 19 / / SYM eurjchem-1439 111 20 ml ml ADP eurjchem-1439 111 21 1.70 1.70 NUM eurjchem-1439 111 22 4.50 4.50 NUM eurjchem-1439 111 23 a a DET eurjchem-1439 111 24 standard standard ADJ eurjchem-1439 111 25 deviation deviation NOUN eurjchem-1439 111 26 , , PUNCT eurjchem-1439 111 27 average average NOUN eurjchem-1439 111 28 of of ADP eurjchem-1439 111 29 three three NUM eurjchem-1439 111 30 determinations determination NOUN eurjchem-1439 111 31 . . PUNCT eurjchem-1439 112 1 b b X eurjchem-1439 112 2 variation variation NOUN eurjchem-1439 112 3 of of ADP eurjchem-1439 112 4 flow flow NOUN eurjchem-1439 112 5 rate rate NOUN eurjchem-1439 112 6 . . PUNCT eurjchem-1439 113 1 c c NOUN eurjchem-1439 113 2 variation variation NOUN eurjchem-1439 113 3 of of ADP eurjchem-1439 113 4 mobile mobile ADJ eurjchem-1439 113 5 phase phase NOUN eurjchem-1439 113 6 composition composition NOUN eurjchem-1439 113 7 . . PUNCT eurjchem-1439 114 1 table table NOUN eurjchem-1439 114 2 2 2 NUM eurjchem-1439 114 3 . . PUNCT eurjchem-1439 114 4 established establish VERB eurjchem-1439 114 5 system system NOUN eurjchem-1439 114 6 suitability suitability NOUN eurjchem-1439 114 7 parameters parameter NOUN eurjchem-1439 114 8 for for ADP eurjchem-1439 114 9 the the DET eurjchem-1439 114 10 hplc hplc NOUN eurjchem-1439 114 11 method method NOUN eurjchem-1439 114 12 . . PUNCT eurjchem-1439 115 1 parameter parameter PROPN eurjchem-1439 115 2 sfv sfv PROPN eurjchem-1439 115 3 peak peak NOUN eurjchem-1439 115 4 significance significance NOUN eurjchem-1439 115 5 tr tr PUNCT eurjchem-1439 115 6 ( ( PUNCT eurjchem-1439 115 7 min min NOUN eurjchem-1439 115 8 ) ) PUNCT eurjchem-1439 115 9 * * PUNCT eurjchem-1439 116 1 1.99±0.05 1.99±0.05 NOUN eurjchem-1439 116 2 ‐ ‐ ADJ eurjchem-1439 116 3 tailing tail VERB eurjchem-1439 116 4 factor factor NOUN eurjchem-1439 116 5 1.1 1.1 NUM eurjchem-1439 116 6 t t NOUN eurjchem-1439 116 7 = = SYM eurjchem-1439 116 8 1 1 NUM eurjchem-1439 116 9 for for ADP eurjchem-1439 116 10 a a DET eurjchem-1439 116 11 typical typical ADJ eurjchem-1439 116 12 symmetric symmetric ADJ eurjchem-1439 116 13 peak peak NOUN eurjchem-1439 116 14 number number NOUN eurjchem-1439 116 15 of of ADP eurjchem-1439 116 16 theoretical theoretical ADJ eurjchem-1439 116 17 plates plate NOUN eurjchem-1439 116 18 ( ( PUNCT eurjchem-1439 116 19 n n CCONJ eurjchem-1439 116 20 ) ) PUNCT eurjchem-1439 116 21 253 253 NUM eurjchem-1439 116 22 increase increase NOUN eurjchem-1439 116 23 with with ADP eurjchem-1439 116 24 the the DET eurjchem-1439 116 25 increase increase NOUN eurjchem-1439 116 26 in in ADP eurjchem-1439 116 27 column column NOUN eurjchem-1439 116 28 efficiency efficiency NOUN eurjchem-1439 116 29 height height NOUN eurjchem-1439 116 30 equivalent equivalent ADJ eurjchem-1439 116 31 to to ADP eurjchem-1439 116 32 theoretical theoretical ADJ eurjchem-1439 116 33 plate plate NOUN eurjchem-1439 116 34 ( ( PUNCT eurjchem-1439 116 35 hetp hetp NOUN eurjchem-1439 116 36 ) ) PUNCT eurjchem-1439 116 37 0.099 0.099 NUM eurjchem-1439 116 38 decrease decrease NOUN eurjchem-1439 116 39 with with ADP eurjchem-1439 116 40 the the DET eurjchem-1439 116 41 increase increase NOUN eurjchem-1439 116 42 in in ADP eurjchem-1439 116 43 column column NOUN eurjchem-1439 116 44 efficiency efficiency NOUN eurjchem-1439 116 45 * * PUNCT eurjchem-1439 116 46 average average NOUN eurjchem-1439 116 47 of of ADP eurjchem-1439 116 48 triplicate triplicate NOUN eurjchem-1439 116 49 runs run NOUN eurjchem-1439 116 50 . . PUNCT eurjchem-1439 116 51 table table NOUN eurjchem-1439 116 52 3 3 NUM eurjchem-1439 116 53 . . PUNCT eurjchem-1439 116 54 determination determination NOUN eurjchem-1439 116 55 of of ADP eurjchem-1439 116 56 sofosbuvir sofosbuvir PROPN eurjchem-1439 116 57 in in ADP eurjchem-1439 116 58 sovaldi sovaldi ADJ eurjchem-1439 116 59 ® ® NOUN eurjchem-1439 116 60 400 400 NUM eurjchem-1439 116 61 mg mg NOUN eurjchem-1439 116 62 film film NOUN eurjchem-1439 116 63 coated coat VERB eurjchem-1439 116 64 tablets tablet NOUN eurjchem-1439 116 65 and and CCONJ eurjchem-1439 116 66 application application NOUN eurjchem-1439 116 67 of of ADP eurjchem-1439 116 68 standard standard ADJ eurjchem-1439 116 69 addition addition NOUN eurjchem-1439 116 70 procedure procedure NOUN eurjchem-1439 116 71 by by ADP eurjchem-1439 116 72 the the DET eurjchem-1439 116 73 proposed propose VERB eurjchem-1439 116 74 methods method NOUN eurjchem-1439 116 75 . . PUNCT eurjchem-1439 117 1 method method PROPN eurjchem-1439 117 2 content content NOUN eurjchem-1439 117 3 uniformity uniformity NOUN eurjchem-1439 117 4 standard standard ADJ eurjchem-1439 117 5 addition addition NOUN eurjchem-1439 117 6 mean±s.d mean±s.d PROPN eurjchem-1439 117 7 . . PUNCT eurjchem-1439 118 1 * * PUNCT eurjchem-1439 118 2 % % NOUN eurjchem-1439 118 3 r.s.d r.s.d NOUN eurjchem-1439 118 4 . . PUNCT eurjchem-1439 119 1 mean±s.d mean±s.d PROPN eurjchem-1439 119 2 . . PUNCT eurjchem-1439 120 1 * * PUNCT eurjchem-1439 120 2 % % NOUN eurjchem-1439 120 3 r.s.d r.s.d NOUN eurjchem-1439 120 4 . . PUNCT eurjchem-1439 121 1 hplc hplc NOUN eurjchem-1439 121 2 method method NOUN eurjchem-1439 121 3 102.08±0.974 102.08±0.974 NUM eurjchem-1439 121 4 0.954 0.954 NUM eurjchem-1439 121 5 101.74±0.786 101.74±0.786 NOUN eurjchem-1439 121 6 0.773 0.773 NUM eurjchem-1439 121 7 uv uv NOUN eurjchem-1439 121 8 spectrophotometric spectrophotometric ADJ eurjchem-1439 121 9 method method NOUN eurjchem-1439 121 10 98.96±0.842 98.96±0.842 NUM eurjchem-1439 121 11 0.851 0.851 NUM eurjchem-1439 121 12 99.74±0.652 99.74±0.652 NUM eurjchem-1439 121 13 0.654 0.654 NUM eurjchem-1439 122 1 * * PUNCT eurjchem-1439 122 2 average average NOUN eurjchem-1439 122 3 of of ADP eurjchem-1439 122 4 three three NUM eurjchem-1439 122 5 determinations determination NOUN eurjchem-1439 122 6 . . PUNCT eurjchem-1439 123 1 where where SCONJ eurjchem-1439 123 2 , , PUNCT eurjchem-1439 123 3 a a PRON eurjchem-1439 123 4 is be AUX eurjchem-1439 123 5 the the DET eurjchem-1439 123 6 absorbance absorbance NOUN eurjchem-1439 123 7 at at ADP eurjchem-1439 123 8 260 260 NUM eurjchem-1439 123 9 nm nm NOUN eurjchem-1439 123 10 and and CCONJ eurjchem-1439 123 11 c c PROPN eurjchem-1439 123 12 is be AUX eurjchem-1439 123 13 the the DET eurjchem-1439 123 14 concent‐ concent‐ NOUN eurjchem-1439 123 15 ration ration NOUN eurjchem-1439 123 16 in in ADP eurjchem-1439 123 17 µg µg ADV eurjchem-1439 123 18 / / SYM eurjchem-1439 123 19 ml ml NOUN eurjchem-1439 123 20 . . PUNCT eurjchem-1439 124 1 3.1.2 3.1.2 X eurjchem-1439 124 2 . . PUNCT eurjchem-1439 125 1 accuracy accuracy NOUN eurjchem-1439 125 2 the the DET eurjchem-1439 125 3 accuracy accuracy NOUN eurjchem-1439 125 4 of of ADP eurjchem-1439 125 5 the the DET eurjchem-1439 125 6 proposed propose VERB eurjchem-1439 125 7 methods method NOUN eurjchem-1439 125 8 was be AUX eurjchem-1439 125 9 validated validate VERB eurjchem-1439 125 10 by by ADP eurjchem-1439 125 11 analyzing analyze VERB eurjchem-1439 125 12 nine nine NUM eurjchem-1439 125 13 quality quality NOUN eurjchem-1439 125 14 control control NOUN eurjchem-1439 125 15 samples sample NOUN eurjchem-1439 125 16 of of ADP eurjchem-1439 125 17 sfv sfv NOUN eurjchem-1439 125 18 representing represent VERB eurjchem-1439 125 19 three three NUM eurjchem-1439 125 20 concentration concentration NOUN eurjchem-1439 125 21 levels level NOUN eurjchem-1439 125 22 covering cover VERB eurjchem-1439 125 23 the the DET eurjchem-1439 125 24 specified specify VERB eurjchem-1439 125 25 linearity linearity NOUN eurjchem-1439 125 26 range range NOUN eurjchem-1439 125 27 then then ADV eurjchem-1439 125 28 calculating calculate VERB eurjchem-1439 125 29 the the DET eurjchem-1439 125 30 recovery recovery NOUN eurjchem-1439 125 31 and and CCONJ eurjchem-1439 125 32 percent percent NOUN eurjchem-1439 125 33 of of ADP eurjchem-1439 125 34 relative relative ADJ eurjchem-1439 125 35 standard standard ADJ eurjchem-1439 125 36 deviation deviation NOUN eurjchem-1439 125 37 ( ( PUNCT eurjchem-1439 125 38 % % INTJ eurjchem-1439 125 39 r.s.d r.s.d NOUN eurjchem-1439 125 40 . . PUNCT eurjchem-1439 125 41 ) ) PUNCT eurjchem-1439 125 42 which which PRON eurjchem-1439 125 43 is be AUX eurjchem-1439 125 44 considered consider VERB eurjchem-1439 125 45 satisfactory satisfactory ADJ eurjchem-1439 125 46 as as SCONJ eurjchem-1439 125 47 it it PRON eurjchem-1439 125 48 was be AUX eurjchem-1439 125 49 less less ADJ eurjchem-1439 125 50 than than ADP eurjchem-1439 125 51 1 1 NUM eurjchem-1439 125 52 % % NOUN eurjchem-1439 125 53 for for ADP eurjchem-1439 125 54 both both DET eurjchem-1439 125 55 methods method NOUN eurjchem-1439 125 56 which which PRON eurjchem-1439 125 57 confirm confirm VERB eurjchem-1439 125 58 the the DET eurjchem-1439 125 59 accuracy accuracy NOUN eurjchem-1439 125 60 of of ADP eurjchem-1439 125 61 the the DET eurjchem-1439 125 62 developed develop VERB eurjchem-1439 125 63 methods method NOUN eurjchem-1439 125 64 ( ( PUNCT eurjchem-1439 125 65 table table NOUN eurjchem-1439 125 66 1 1 NUM eurjchem-1439 125 67 ) ) PUNCT eurjchem-1439 125 68 . . PUNCT eurjchem-1439 126 1 3.1.3 3.1.3 NUM eurjchem-1439 126 2 . . PUNCT eurjchem-1439 126 3 precision precision VERB eurjchem-1439 126 4 the the DET eurjchem-1439 126 5 intraday intraday NOUN eurjchem-1439 126 6 and and CCONJ eurjchem-1439 126 7 interday interday NOUN eurjchem-1439 126 8 precisions precision NOUN eurjchem-1439 126 9 were be AUX eurjchem-1439 126 10 checked check VERB eurjchem-1439 126 11 by by ADP eurjchem-1439 126 12 analyzing analyze VERB eurjchem-1439 126 13 three three NUM eurjchem-1439 126 14 different different ADJ eurjchem-1439 126 15 concentrations concentration NOUN eurjchem-1439 126 16 of of ADP eurjchem-1439 126 17 sfv sfv NOUN eurjchem-1439 126 18 by by ADP eurjchem-1439 126 19 adopting adopt VERB eurjchem-1439 126 20 the the DET eurjchem-1439 126 21 proposed propose VERB eurjchem-1439 126 22 methods method NOUN eurjchem-1439 126 23 , , PUNCT eurjchem-1439 126 24 either either CCONJ eurjchem-1439 126 25 in in ADP eurjchem-1439 126 26 the the DET eurjchem-1439 126 27 same same ADJ eurjchem-1439 126 28 day day NOUN eurjchem-1439 126 29 or or CCONJ eurjchem-1439 126 30 during during ADP eurjchem-1439 126 31 three three NUM eurjchem-1439 126 32 successive successive ADJ eurjchem-1439 126 33 days day NOUN eurjchem-1439 126 34 . . PUNCT eurjchem-1439 127 1 the the DET eurjchem-1439 127 2 % % NOUN eurjchem-1439 127 3 r.s.d r.s.d NOUN eurjchem-1439 127 4 . . PUNCT eurjchem-1439 128 1 values value NOUN eurjchem-1439 128 2 for for ADP eurjchem-1439 128 3 intraday intraday NOUN eurjchem-1439 128 4 and and CCONJ eurjchem-1439 128 5 interday interday NOUN eurjchem-1439 128 6 precisions precision NOUN eurjchem-1439 128 7 were be AUX eurjchem-1439 128 8 less less ADJ eurjchem-1439 128 9 than than ADP eurjchem-1439 128 10 2 2 NUM eurjchem-1439 128 11 % % NOUN eurjchem-1439 128 12 for for ADP eurjchem-1439 128 13 the the DET eurjchem-1439 128 14 proposed propose VERB eurjchem-1439 128 15 methods method NOUN eurjchem-1439 128 16 , , PUNCT eurjchem-1439 128 17 which which PRON eurjchem-1439 128 18 confirm confirm VERB eurjchem-1439 128 19 the the DET eurjchem-1439 128 20 good good ADJ eurjchem-1439 128 21 precision precision NOUN eurjchem-1439 128 22 of of ADP eurjchem-1439 128 23 both both DET eurjchem-1439 128 24 methods method NOUN eurjchem-1439 128 25 ( ( PUNCT eurjchem-1439 128 26 table table NOUN eurjchem-1439 128 27 1 1 NUM eurjchem-1439 128 28 ) ) PUNCT eurjchem-1439 128 29 . . PUNCT eurjchem-1439 129 1 3.1.4 3.1.4 X eurjchem-1439 129 2 . . PUNCT eurjchem-1439 129 3 specificity specificity NOUN eurjchem-1439 129 4 the the DET eurjchem-1439 129 5 proposed propose VERB eurjchem-1439 129 6 methods method NOUN eurjchem-1439 129 7 were be AUX eurjchem-1439 129 8 specific specific ADJ eurjchem-1439 129 9 as as SCONJ eurjchem-1439 129 10 none none NOUN eurjchem-1439 129 11 of of ADP eurjchem-1439 129 12 the the DET eurjchem-1439 129 13 tried try VERB eurjchem-1439 129 14 excipients excipient NOUN eurjchem-1439 129 15 interfered interfere VERB eurjchem-1439 129 16 with with ADP eurjchem-1439 129 17 the the DET eurjchem-1439 129 18 drug drug NOUN eurjchem-1439 129 19 of of ADP eurjchem-1439 129 20 interest interest NOUN eurjchem-1439 129 21 , , PUNCT eurjchem-1439 129 22 so so ADV eurjchem-1439 129 23 , , PUNCT eurjchem-1439 129 24 the the DET eurjchem-1439 129 25 methods method NOUN eurjchem-1439 129 26 were be AUX eurjchem-1439 129 27 suitably suitably ADV eurjchem-1439 129 28 applied apply VERB eurjchem-1439 129 29 for for ADP eurjchem-1439 129 30 assaying assay VERB eurjchem-1439 129 31 the the DET eurjchem-1439 129 32 commercial commercial ADJ eurjchem-1439 129 33 product product NOUN eurjchem-1439 129 34 . . PUNCT eurjchem-1439 130 1 3.1.5 3.1.5 X eurjchem-1439 130 2 . . PUNCT eurjchem-1439 130 3 limits limit NOUN eurjchem-1439 130 4 of of ADP eurjchem-1439 130 5 detection detection NOUN eurjchem-1439 130 6 and and CCONJ eurjchem-1439 130 7 quantification quantification NOUN eurjchem-1439 130 8 the the DET eurjchem-1439 130 9 obtained obtain VERB eurjchem-1439 130 10 values value NOUN eurjchem-1439 130 11 of of ADP eurjchem-1439 130 12 lod lod PROPN eurjchem-1439 130 13 and and CCONJ eurjchem-1439 130 14 loq loq PROPN eurjchem-1439 130 15 confirmed confirm VERB eurjchem-1439 130 16 the the DET eurjchem-1439 130 17 sufficient sufficient ADJ eurjchem-1439 130 18 sensitivity sensitivity NOUN eurjchem-1439 130 19 of of ADP eurjchem-1439 130 20 the the DET eurjchem-1439 130 21 proposed propose VERB eurjchem-1439 130 22 methods method NOUN eurjchem-1439 130 23 ( ( PUNCT eurjchem-1439 130 24 table table NOUN eurjchem-1439 130 25 1 1 NUM eurjchem-1439 130 26 ) ) PUNCT eurjchem-1439 130 27 . . PUNCT eurjchem-1439 131 1 3.1.6 3.1.6 X eurjchem-1439 131 2 . . X eurjchem-1439 132 1 robustness robustness NOUN eurjchem-1439 132 2 it it PRON eurjchem-1439 132 3 is be AUX eurjchem-1439 132 4 a a DET eurjchem-1439 132 5 measure measure NOUN eurjchem-1439 132 6 of of ADP eurjchem-1439 132 7 the the DET eurjchem-1439 132 8 method method NOUN eurjchem-1439 132 9 capability capability NOUN eurjchem-1439 132 10 to to PART eurjchem-1439 132 11 maintain maintain VERB eurjchem-1439 132 12 unaffected unaffected ADJ eurjchem-1439 132 13 by by ADP eurjchem-1439 132 14 slight slight ADJ eurjchem-1439 132 15 changes change NOUN eurjchem-1439 132 16 in in ADP eurjchem-1439 132 17 its its PRON eurjchem-1439 132 18 parameters parameter NOUN eurjchem-1439 132 19 . . PUNCT eurjchem-1439 133 1 the the DET eurjchem-1439 133 2 studied study VERB eurjchem-1439 133 3 parameters parameter NOUN eurjchem-1439 133 4 were be AUX eurjchem-1439 133 5 the the DET eurjchem-1439 133 6 variation variation NOUN eurjchem-1439 133 7 in in ADP eurjchem-1439 133 8 flow flow NOUN eurjchem-1439 133 9 rate rate NOUN eurjchem-1439 133 10 or or CCONJ eurjchem-1439 133 11 mobile mobile ADJ eurjchem-1439 133 12 phase phase NOUN eurjchem-1439 133 13 composition composition NOUN eurjchem-1439 133 14 ( ( PUNCT eurjchem-1439 133 15 hplc hplc NOUN eurjchem-1439 133 16 method method NOUN eurjchem-1439 133 17 ) ) PUNCT eurjchem-1439 133 18 and and CCONJ eurjchem-1439 133 19 solvent solvent NOUN eurjchem-1439 133 20 composition composition NOUN eurjchem-1439 133 21 ( ( PUNCT eurjchem-1439 133 22 spectrophotometric spectrophotometric ADJ eurjchem-1439 133 23 method method NOUN eurjchem-1439 133 24 ) ) PUNCT eurjchem-1439 133 25 . . PUNCT eurjchem-1439 134 1 the the DET eurjchem-1439 134 2 proposed propose VERB eurjchem-1439 134 3 methods method NOUN eurjchem-1439 134 4 were be AUX eurjchem-1439 134 5 not not PART eurjchem-1439 134 6 affected affect VERB eurjchem-1439 134 7 by by ADP eurjchem-1439 134 8 the the DET eurjchem-1439 134 9 slight slight ADJ eurjchem-1439 134 10 changes change NOUN eurjchem-1439 134 11 in in ADP eurjchem-1439 134 12 their their PRON eurjchem-1439 134 13 conditions condition NOUN eurjchem-1439 134 14 where where SCONJ eurjchem-1439 134 15 the the DET eurjchem-1439 134 16 % % NOUN eurjchem-1439 134 17 r.s.d r.s.d NOUN eurjchem-1439 134 18 . . PUNCT eurjchem-1439 135 1 values value NOUN eurjchem-1439 135 2 were be AUX eurjchem-1439 135 3 less less ADJ eurjchem-1439 135 4 than than ADP eurjchem-1439 135 5 1 1 NUM eurjchem-1439 135 6 % % NOUN eurjchem-1439 135 7 and and CCONJ eurjchem-1439 135 8 so so ADV eurjchem-1439 135 9 this this PRON eurjchem-1439 135 10 confirms confirm VERB eurjchem-1439 135 11 the the DET eurjchem-1439 135 12 robustness robustness NOUN eurjchem-1439 135 13 of of ADP eurjchem-1439 135 14 the the DET eurjchem-1439 135 15 methods method NOUN eurjchem-1439 135 16 ( ( PUNCT eurjchem-1439 135 17 table table NOUN eurjchem-1439 135 18 1 1 NUM eurjchem-1439 135 19 ) ) PUNCT eurjchem-1439 135 20 . . PUNCT eurjchem-1439 136 1 3.1.7 3.1.7 X eurjchem-1439 136 2 . . PUNCT eurjchem-1439 136 3 system system NOUN eurjchem-1439 136 4 suitability suitability NOUN eurjchem-1439 136 5 system system NOUN eurjchem-1439 136 6 suitability suitability NOUN eurjchem-1439 136 7 parameters parameter NOUN eurjchem-1439 136 8 for for ADP eurjchem-1439 136 9 the the DET eurjchem-1439 136 10 chromatographic chromatographic ADJ eurjchem-1439 136 11 method method NOUN eurjchem-1439 136 12 were be AUX eurjchem-1439 136 13 studied study VERB eurjchem-1439 136 14 , , PUNCT eurjchem-1439 136 15 to to PART eurjchem-1439 136 16 evaluate evaluate VERB eurjchem-1439 136 17 the the DET eurjchem-1439 136 18 peak peak NOUN eurjchem-1439 136 19 symmetry symmetry NOUN eurjchem-1439 136 20 and and CCONJ eurjchem-1439 136 21 column column NOUN eurjchem-1439 136 22 efficiency efficiency NOUN eurjchem-1439 136 23 . . PUNCT eurjchem-1439 137 1 the the DET eurjchem-1439 137 2 studied study VERB eurjchem-1439 137 3 parameters parameter NOUN eurjchem-1439 137 4 were be AUX eurjchem-1439 137 5 tailing tail VERB eurjchem-1439 137 6 factor factor NOUN eurjchem-1439 137 7 , , PUNCT eurjchem-1439 137 8 number number NOUN eurjchem-1439 137 9 of of ADP eurjchem-1439 137 10 theoretical theoretical ADJ eurjchem-1439 137 11 plates plate NOUN eurjchem-1439 137 12 ( ( PUNCT eurjchem-1439 137 13 n n CCONJ eurjchem-1439 137 14 ) ) PUNCT eurjchem-1439 137 15 and and CCONJ eurjchem-1439 137 16 height height NOUN eurjchem-1439 137 17 equivalent equivalent ADJ eurjchem-1439 137 18 to to AUX eurjchem-1439 137 19 theo‐ theo‐ VERB eurjchem-1439 137 20 retical retical ADJ eurjchem-1439 137 21 plates plate NOUN eurjchem-1439 137 22 ( ( PUNCT eurjchem-1439 137 23 hetp hetp NOUN eurjchem-1439 137 24 ) ) PUNCT eurjchem-1439 137 25 . . PUNCT eurjchem-1439 138 1 the the DET eurjchem-1439 138 2 values value NOUN eurjchem-1439 138 3 of of ADP eurjchem-1439 138 4 the the DET eurjchem-1439 138 5 studied study VERB eurjchem-1439 138 6 parameters parameter NOUN eurjchem-1439 138 7 confirmed confirm VERB eurjchem-1439 138 8 the the DET eurjchem-1439 138 9 excellent excellent ADJ eurjchem-1439 138 10 peak peak NOUN eurjchem-1439 138 11 symmetry symmetry NOUN eurjchem-1439 138 12 and and CCONJ eurjchem-1439 138 13 high high ADJ eurjchem-1439 138 14 column column NOUN eurjchem-1439 138 15 efficiency efficiency NOUN eurjchem-1439 138 16 , , PUNCT eurjchem-1439 138 17 table table NOUN eurjchem-1439 138 18 2 2 NUM eurjchem-1439 138 19 . . NOUN eurjchem-1439 138 20 3.2 3.2 NUM eurjchem-1439 138 21 . . PUNCT eurjchem-1439 139 1 analysis analysis NOUN eurjchem-1439 139 2 of of ADP eurjchem-1439 139 3 pharmaceutical pharmaceutical ADJ eurjchem-1439 139 4 formulation formulation NOUN eurjchem-1439 139 5 the the DET eurjchem-1439 139 6 proposed propose VERB eurjchem-1439 139 7 methods method NOUN eurjchem-1439 139 8 were be AUX eurjchem-1439 139 9 successfully successfully ADV eurjchem-1439 139 10 adopted adopt VERB eurjchem-1439 139 11 for for ADP eurjchem-1439 139 12 the the DET eurjchem-1439 139 13 quantification quantification NOUN eurjchem-1439 139 14 of of ADP eurjchem-1439 139 15 sfv sfv PROPN eurjchem-1439 139 16 in in ADP eurjchem-1439 139 17 sovaldi sovaldi ADJ eurjchem-1439 139 18 ® ® NOUN eurjchem-1439 139 19 400 400 NUM eurjchem-1439 139 20 mg mg NOUN eurjchem-1439 139 21 film film NOUN eurjchem-1439 139 22 coated coat VERB eurjchem-1439 139 23 tablets tablet NOUN eurjchem-1439 139 24 to to PART eurjchem-1439 139 25 assess assess VERB eurjchem-1439 139 26 content content NOUN eurjchem-1439 139 27 uniformity uniformity NOUN eurjchem-1439 139 28 ( ( PUNCT eurjchem-1439 139 29 table table NOUN eurjchem-1439 139 30 3 3 NUM eurjchem-1439 139 31 ) ) PUNCT eurjchem-1439 139 32 . . PUNCT eurjchem-1439 140 1 the the DET eurjchem-1439 140 2 commercial commercial ADJ eurjchem-1439 140 3 dosage dosage NOUN eurjchem-1439 140 4 form form NOUN eurjchem-1439 140 5 showed show VERB eurjchem-1439 140 6 acceptable acceptable ADJ eurjchem-1439 140 7 recoveries recovery NOUN eurjchem-1439 140 8 by by ADP eurjchem-1439 140 9 applying apply VERB eurjchem-1439 140 10 the the DET eurjchem-1439 140 11 proposed propose VERB eurjchem-1439 140 12 methods method NOUN eurjchem-1439 140 13 which which PRON eurjchem-1439 140 14 showed show VERB eurjchem-1439 140 15 acceptable acceptable ADJ eurjchem-1439 140 16 limits limit NOUN eurjchem-1439 140 17 of of ADP eurjchem-1439 140 18 content content NOUN eurjchem-1439 140 19 uniform‐ uniform‐ NOUN eurjchem-1439 140 20 mity mity NOUN eurjchem-1439 140 21 . . PUNCT eurjchem-1439 141 1 also also ADV eurjchem-1439 141 2 , , PUNCT eurjchem-1439 141 3 the the DET eurjchem-1439 141 4 standard standard ADJ eurjchem-1439 141 5 addition addition NOUN eurjchem-1439 141 6 procedure procedure NOUN eurjchem-1439 141 7 was be AUX eurjchem-1439 141 8 applied apply VERB eurjchem-1439 141 9 by by ADP eurjchem-1439 141 10 spiking spike VERB eurjchem-1439 141 11 different different ADJ eurjchem-1439 141 12 known know VERB eurjchem-1439 141 13 quantities quantity NOUN eurjchem-1439 141 14 of of ADP eurjchem-1439 141 15 pure pure ADJ eurjchem-1439 141 16 sfv sfv PROPN eurjchem-1439 141 17 to to ADP eurjchem-1439 141 18 the the DET eurjchem-1439 141 19 tablet tablet ADJ eurjchem-1439 141 20 formulation formulation NOUN eurjchem-1439 141 21 to to PART eurjchem-1439 141 22 ensure ensure VERB eurjchem-1439 141 23 the the DET eurjchem-1439 141 24 applicability applicability NOUN eurjchem-1439 141 25 and and CCONJ eurjchem-1439 141 26 reliability reliability NOUN eurjchem-1439 141 27 of of ADP eurjchem-1439 141 28 the the DET eurjchem-1439 141 29 proposed propose VERB eurjchem-1439 141 30 methods method NOUN eurjchem-1439 141 31 . . PUNCT eurjchem-1439 142 1 the the DET eurjchem-1439 142 2 results result NOUN eurjchem-1439 142 3 showed show VERB eurjchem-1439 142 4 satisfactory satisfactory ADJ eurjchem-1439 142 5 recoveries recovery NOUN eurjchem-1439 142 6 of of ADP eurjchem-1439 142 7 the the DET eurjchem-1439 142 8 pure pure ADJ eurjchem-1439 142 9 added add VERB eurjchem-1439 142 10 drug drug NOUN eurjchem-1439 142 11 by by ADP eurjchem-1439 142 12 the the DET eurjchem-1439 142 13 proposed propose VERB eurjchem-1439 142 14 methods method NOUN eurjchem-1439 142 15 ( ( PUNCT eurjchem-1439 142 16 table table NOUN eurjchem-1439 142 17 3 3 NUM eurjchem-1439 142 18 ) ) PUNCT eurjchem-1439 142 19 . . PUNCT eurjchem-1439 143 1 4 4 X eurjchem-1439 143 2 . . X eurjchem-1439 143 3 conclusion conclusion NOUN eurjchem-1439 143 4 the the DET eurjchem-1439 143 5 proposed propose VERB eurjchem-1439 143 6 rp‐hplc rp‐hplc PROPN eurjchem-1439 143 7 and and CCONJ eurjchem-1439 143 8 uv uv NOUN eurjchem-1439 143 9 spectrophotometric spectrophotometric ADJ eurjchem-1439 143 10 methods method NOUN eurjchem-1439 143 11 are be AUX eurjchem-1439 143 12 simple simple ADJ eurjchem-1439 143 13 , , PUNCT eurjchem-1439 143 14 sensitive sensitive ADJ eurjchem-1439 143 15 and and CCONJ eurjchem-1439 143 16 selective selective ADJ eurjchem-1439 143 17 having have VERB eurjchem-1439 143 18 acceptable acceptable ADJ eurjchem-1439 143 19 accuracy accuracy NOUN eurjchem-1439 143 20 and and CCONJ eurjchem-1439 143 21 precision precision NOUN eurjchem-1439 143 22 . . PUNCT eurjchem-1439 144 1 also also ADV eurjchem-1439 144 2 , , PUNCT eurjchem-1439 144 3 the the DET eurjchem-1439 144 4 short short ADJ eurjchem-1439 144 5 analysis analysis NOUN eurjchem-1439 144 6 time time NOUN eurjchem-1439 144 7 for for ADP eurjchem-1439 144 8 both both DET eurjchem-1439 144 9 methods method NOUN eurjchem-1439 144 10 makes make VERB eurjchem-1439 144 11 them they PRON eurjchem-1439 144 12 suitable suitable ADJ eurjchem-1439 144 13 for for ADP eurjchem-1439 144 14 the the DET eurjchem-1439 144 15 assay assay NOUN eurjchem-1439 144 16 of of ADP eurjchem-1439 144 17 sfv sfv PROPN eurjchem-1439 144 18 in in ADP eurjchem-1439 144 19 its its PRON eurjchem-1439 144 20 commercial commercial ADJ eurjchem-1439 144 21 product product NOUN eurjchem-1439 144 22 during during ADP eurjchem-1439 144 23 the the DET eurjchem-1439 144 24 routine routine ADJ eurjchem-1439 144 25 analysis analysis NOUN eurjchem-1439 144 26 in in ADP eurjchem-1439 144 27 quality quality NOUN eurjchem-1439 144 28 control control NOUN eurjchem-1439 144 29 laboratories laboratory NOUN eurjchem-1439 144 30 . . PUNCT eurjchem-1439 145 1 moreover moreover ADV eurjchem-1439 145 2 , , PUNCT eurjchem-1439 145 3 the the DET eurjchem-1439 145 4 uv‐spectrophotometric uv‐spectrophotometric ADJ eurjchem-1439 145 5 sherif sherif NOUN eurjchem-1439 145 6 abdel‐naby abdel‐naby PROPN eurjchem-1439 145 7 abdel‐gawad abdel‐gawad PROPN eurjchem-1439 145 8 / / SYM eurjchem-1439 145 9 european european PROPN eurjchem-1439 145 10 journal journal PROPN eurjchem-1439 145 11 of of ADP eurjchem-1439 145 12 chemistry chemistry NOUN eurjchem-1439 145 13 7 7 NUM eurjchem-1439 145 14 ( ( PUNCT eurjchem-1439 145 15 3 3 NUM eurjchem-1439 145 16 ) ) PUNCT eurjchem-1439 145 17 ( ( PUNCT eurjchem-1439 145 18 2016 2016 NUM eurjchem-1439 145 19 ) ) PUNCT eurjchem-1439 145 20 375‐379 375‐379 PROPN eurjchem-1439 145 21 379 379 NUM eurjchem-1439 145 22 method method NOUN eurjchem-1439 145 23 offers offer VERB eurjchem-1439 145 24 a a DET eurjchem-1439 145 25 cost cost NOUN eurjchem-1439 145 26 effective effective ADJ eurjchem-1439 145 27 alternative alternative NOUN eurjchem-1439 145 28 to to ADP eurjchem-1439 145 29 the the DET eurjchem-1439 145 30 rp‐hplc rp‐hplc PROPN eurjchem-1439 145 31 method method NOUN eurjchem-1439 145 32 of of ADP eurjchem-1439 145 33 analysis analysis NOUN eurjchem-1439 145 34 . . PUNCT eurjchem-1439 146 1 acknowledgements acknowledgement NOUN eurjchem-1439 146 2 i i PRON eurjchem-1439 146 3 gratefully gratefully ADV eurjchem-1439 146 4 acknowledge acknowledge VERB eurjchem-1439 146 5 the the DET eurjchem-1439 146 6 central central ADJ eurjchem-1439 146 7 laboratory laboratory NOUN eurjchem-1439 146 8 ( ( PUNCT eurjchem-1439 146 9 collage collage NOUN eurjchem-1439 146 10 of of ADP eurjchem-1439 146 11 pharmacy pharmacy NOUN eurjchem-1439 146 12 , , PUNCT eurjchem-1439 146 13 prince prince PROPN eurjchem-1439 146 14 sattam sattam PROPN eurjchem-1439 146 15 bin‐abdul bin‐abdul PROPN eurjchem-1439 146 16 aziz aziz PROPN eurjchem-1439 146 17 university university PROPN eurjchem-1439 146 18 , , PUNCT eurjchem-1439 146 19 al‐kharj al‐kharj VERB eurjchem-1439 146 20 , , PUNCT eurjchem-1439 146 21 kingdom kingdom NOUN eurjchem-1439 146 22 of of ADP eurjchem-1439 146 23 saudi saudi PROPN eurjchem-1439 146 24 arabia arabia PROPN eurjchem-1439 146 25 ) ) PUNCT eurjchem-1439 146 26 for for ADP eurjchem-1439 146 27 providing provide VERB eurjchem-1439 146 28 essential essential ADJ eurjchem-1439 146 29 services service NOUN eurjchem-1439 146 30 to to PART eurjchem-1439 146 31 carry carry VERB eurjchem-1439 146 32 out out ADP eurjchem-1439 146 33 this this DET eurjchem-1439 146 34 work work NOUN eurjchem-1439 146 35 . . PUNCT eurjchem-1439 147 1 references reference NOUN eurjchem-1439 147 2 [ [ X eurjchem-1439 147 3 1 1 NUM eurjchem-1439 147 4 ] ] PUNCT eurjchem-1439 147 5 . . PUNCT eurjchem-1439 148 1 asselah asselah PROPN eurjchem-1439 148 2 , , PUNCT eurjchem-1439 148 3 t. t. NOUN eurjchem-1439 148 4 expert expert NOUN eurjchem-1439 148 5 . . PUNCT eurjchem-1439 149 1 opin opin NOUN eurjchem-1439 149 2 . . PUNCT eurjchem-1439 150 1 pharmacother pharmacother PROPN eurjchem-1439 150 2 . . PUNCT eurjchem-1439 151 1 2014 2014 NUM eurjchem-1439 151 2 , , PUNCT eurjchem-1439 151 3 15 15 NUM eurjchem-1439 151 4 , , PUNCT eurjchem-1439 151 5 121‐130 121‐130 NOUN eurjchem-1439 151 6 . . PUNCT eurjchem-1439 152 1 [ [ X eurjchem-1439 152 2 2 2 NUM eurjchem-1439 152 3 ] ] PUNCT eurjchem-1439 152 4 . . PUNCT eurjchem-1439 153 1 herbst herbst PROPN eurjchem-1439 153 2 , , PUNCT eurjchem-1439 153 3 j. j. PROPN eurjchem-1439 153 4 d. d. PROPN eurjchem-1439 153 5 a. a. PROPN eurjchem-1439 153 6 ; ; PUNCT eurjchem-1439 153 7 reddy reddy PROPN eurjchem-1439 153 8 , , PUNCT eurjchem-1439 153 9 k. k. PROPN eurjchem-1439 153 10 r. r. PROPN eurjchem-1439 153 11 expert expert PROPN eurjchem-1439 153 12 . . PUNCT eurjchem-1439 154 1 opin opin NOUN eurjchem-1439 154 2 . . PUNCT eurjchem-1439 155 1 investig investig PROPN eurjchem-1439 155 2 . . PUNCT eurjchem-1439 156 1 drugs drug NOUN eurjchem-1439 156 2 2013 2013 NUM eurjchem-1439 156 3 , , PUNCT eurjchem-1439 156 4 22 22 NUM eurjchem-1439 156 5 , , PUNCT eurjchem-1439 156 6 527‐536 527‐536 NUM eurjchem-1439 156 7 . . PUNCT eurjchem-1439 157 1 [ [ X eurjchem-1439 157 2 3 3 NUM eurjchem-1439 157 3 ] ] PUNCT eurjchem-1439 157 4 . . PUNCT eurjchem-1439 158 1 gane gane NOUN eurjchem-1439 158 2 , , PUNCT eurjchem-1439 158 3 e. e. PROPN eurjchem-1439 158 4 j. j. PROPN eurjchem-1439 158 5 ; ; PUNCT eurjchem-1439 158 6 stedman stedman PROPN eurjchem-1439 158 7 , , PUNCT eurjchem-1439 158 8 c. c. PROPN eurjchem-1439 158 9 a. a. PROPN eurjchem-1439 158 10 ; ; PUNCT eurjchem-1439 158 11 hyland hyland PROPN eurjchem-1439 158 12 , , PUNCT eurjchem-1439 158 13 r. r. PROPN eurjchem-1439 158 14 h. h. PROPN eurjchem-1439 158 15 ; ; PUNCT eurjchem-1439 158 16 ding ding NOUN eurjchem-1439 158 17 , , PUNCT eurjchem-1439 158 18 x. x. NOUN eurjchem-1439 158 19 ; ; PUNCT eurjchem-1439 158 20 svarovskaia svarovskaia PROPN eurjchem-1439 158 21 , , PUNCT eurjchem-1439 158 22 e. e. PROPN eurjchem-1439 158 23 ; ; PUNCT eurjchem-1439 158 24 symonds symonds PROPN eurjchem-1439 158 25 , , PUNCT eurjchem-1439 158 26 w. w. PROPN eurjchem-1439 158 27 t. t. PROPN eurjchem-1439 158 28 ; ; PUNCT eurjchem-1439 158 29 hindes hinde NOUN eurjchem-1439 158 30 , , PUNCT eurjchem-1439 158 31 r. r. PROPN eurjchem-1439 158 32 g. g. PROPN eurjchem-1439 158 33 ; ; PUNCT eurjchem-1439 158 34 berrey berrey PROPN eurjchem-1439 158 35 , , PUNCT eurjchem-1439 158 36 m. m. NOUN eurjchem-1439 158 37 m. m. PROPN eurjchem-1439 158 38 n. n. PROPN eurjchem-1439 158 39 engl engl PROPN eurjchem-1439 158 40 . . PUNCT eurjchem-1439 159 1 j. j. PROPN eurjchem-1439 159 2 med med PROPN eurjchem-1439 159 3 . . PUNCT eurjchem-1439 159 4 2013 2013 NUM eurjchem-1439 159 5 , , PUNCT eurjchem-1439 159 6 368 368 NUM eurjchem-1439 159 7 , , PUNCT eurjchem-1439 159 8 34‐44 34‐44 NUM eurjchem-1439 159 9 . . PUNCT eurjchem-1439 160 1 [ [ X eurjchem-1439 160 2 4 4 NUM eurjchem-1439 160 3 ] ] PUNCT eurjchem-1439 160 4 . . PUNCT eurjchem-1439 161 1 http://www.who.int/selection_medicines/committees/expert/20/a http://www.who.int/selection_medicines/committees/expert/20/a PROPN eurjchem-1439 161 2 pplications pplication NOUN eurjchem-1439 161 3 / / SYM eurjchem-1439 161 4 ledipasvirfdc.pdf ledipasvirfdc.pdf PROPN eurjchem-1439 161 5 , , PUNCT eurjchem-1439 161 6 accessed access VERB eurjchem-1439 161 7 10 10 NUM eurjchem-1439 161 8 april april PROPN eurjchem-1439 161 9 2016 2016 NUM eurjchem-1439 161 10 . . PUNCT eurjchem-1439 162 1 [ [ X eurjchem-1439 162 2 5 5 NUM eurjchem-1439 162 3 ] ] PUNCT eurjchem-1439 162 4 . . PUNCT eurjchem-1439 163 1 shi shi PROPN eurjchem-1439 163 2 , , PUNCT eurjchem-1439 163 3 x. x. PROPN eurjchem-1439 163 4 ; ; PUNCT eurjchem-1439 163 5 zhu zhu PROPN eurjchem-1439 163 6 , , PUNCT eurjchem-1439 163 7 d. d. PROPN eurjchem-1439 163 8 ; ; PUNCT eurjchem-1439 163 9 lou lou PROPN eurjchem-1439 163 10 , , PUNCT eurjchem-1439 163 11 j. j. PROPN eurjchem-1439 163 12 ; ; PUNCT eurjchem-1439 163 13 zhu zhu PROPN eurjchem-1439 163 14 , , PUNCT eurjchem-1439 163 15 b. b. PROPN eurjchem-1439 163 16 ; ; PUNCT eurjchem-1439 163 17 hu hu PROPN eurjchem-1439 163 18 , , PUNCT eurjchem-1439 163 19 a. a. PROPN eurjchem-1439 163 20 r. r. PROPN eurjchem-1439 163 21 ; ; PUNCT eurjchem-1439 163 22 gan gan PROPN eurjchem-1439 163 23 , , PUNCT eurjchem-1439 163 24 d. d. PROPN eurjchem-1439 163 25 j. j. PROPN eurjchem-1439 163 26 chromatogr chromatogr PROPN eurjchem-1439 163 27 . . PUNCT eurjchem-1439 164 1 b b NOUN eurjchem-1439 164 2 2015 2015 NUM eurjchem-1439 164 3 , , PUNCT eurjchem-1439 164 4 1002 1002 NUM eurjchem-1439 164 5 , , PUNCT eurjchem-1439 164 6 353‐357 353‐357 NUM eurjchem-1439 164 7 . . PUNCT eurjchem-1439 165 1 [ [ X eurjchem-1439 165 2 6 6 NUM eurjchem-1439 165 3 ] ] PUNCT eurjchem-1439 165 4 . . PUNCT eurjchem-1439 166 1 pan pan PROPN eurjchem-1439 166 2 , , PUNCT eurjchem-1439 166 3 c. c. PROPN eurjchem-1439 166 4 ; ; PUNCT eurjchem-1439 166 5 chen chen PROPN eurjchem-1439 166 6 , , PUNCT eurjchem-1439 166 7 y. y. PROPN eurjchem-1439 166 8 ; ; PUNCT eurjchem-1439 166 9 chen chen PROPN eurjchem-1439 166 10 , , PUNCT eurjchem-1439 166 11 w. w. PROPN eurjchem-1439 166 12 ; ; PUNCT eurjchem-1439 166 13 zhou zhou PROPN eurjchem-1439 166 14 , , PUNCT eurjchem-1439 166 15 g. g. PROPN eurjchem-1439 166 16 ; ; PUNCT eurjchem-1439 166 17 jin jin PROPN eurjchem-1439 166 18 , , PUNCT eurjchem-1439 166 19 l. l. PROPN eurjchem-1439 166 20 ; ; PUNCT eurjchem-1439 166 21 zheng zheng PROPN eurjchem-1439 166 22 , , PUNCT eurjchem-1439 166 23 y. y. PROPN eurjchem-1439 166 24 ; ; PUNCT eurjchem-1439 166 25 lin lin PROPN eurjchem-1439 166 26 , , PUNCT eurjchem-1439 166 27 w. w. PROPN eurjchem-1439 166 28 ; ; PUNCT eurjchem-1439 166 29 pan pan PROPN eurjchem-1439 166 30 , , PUNCT eurjchem-1439 166 31 z. z. PROPN eurjchem-1439 166 32 j. j. PROPN eurjchem-1439 166 33 chromatogr chromatogr PROPN eurjchem-1439 166 34 . . PUNCT eurjchem-1439 167 1 b b ADP eurjchem-1439 167 2 2016 2016 NUM eurjchem-1439 167 3 , , PUNCT eurjchem-1439 167 4 1008 1008 NUM eurjchem-1439 167 5 , , PUNCT eurjchem-1439 167 6 255‐259 255‐259 NUM eurjchem-1439 167 7 . . PUNCT eurjchem-1439 168 1 [ [ X eurjchem-1439 168 2 7 7 NUM eurjchem-1439 168 3 ] ] PUNCT eurjchem-1439 168 4 . . PUNCT eurjchem-1439 169 1 rezk rezk PROPN eurjchem-1439 169 2 , , PUNCT eurjchem-1439 169 3 m. m. PROPN eurjchem-1439 169 4 r. r. PROPN eurjchem-1439 169 5 ; ; PUNCT eurjchem-1439 169 6 basalious basalious ADJ eurjchem-1439 169 7 , , PUNCT eurjchem-1439 169 8 e. e. PROPN eurjchem-1439 169 9 b. b. PROPN eurjchem-1439 169 10 ; ; PUNCT eurjchem-1439 169 11 karim karim PROPN eurjchem-1439 169 12 , , PUNCT eurjchem-1439 169 13 i. i. PROPN eurjchem-1439 169 14 a. a. PROPN eurjchem-1439 169 15 j. j. PROPN eurjchem-1439 169 16 pharm pharm PROPN eurjchem-1439 169 17 . . PUNCT eurjchem-1439 169 18 biomed biome VERB eurjchem-1439 169 19 . . PUNCT eurjchem-1439 170 1 anal anal ADJ eurjchem-1439 170 2 . . PUNCT eurjchem-1439 171 1 2015 2015 NUM eurjchem-1439 171 2 , , PUNCT eurjchem-1439 171 3 117 117 NUM eurjchem-1439 171 4 , , PUNCT eurjchem-1439 171 5 97‐104 97‐104 NOUN eurjchem-1439 171 6 . . PUNCT eurjchem-1439 172 1 [ [ X eurjchem-1439 172 2 8 8 NUM eurjchem-1439 172 3 ] ] PUNCT eurjchem-1439 172 4 . . PUNCT eurjchem-1439 173 1 ich ich NOUN eurjchem-1439 173 2 topic topic NOUN eurjchem-1439 173 3 q2 q2 NOUN eurjchem-1439 173 4 ( ( PUNCT eurjchem-1439 173 5 r1 r1 PROPN eurjchem-1439 173 6 ) ) PUNCT eurjchem-1439 173 7 validation validation NOUN eurjchem-1439 173 8 of of ADP eurjchem-1439 173 9 analytical analytical ADJ eurjchem-1439 173 10 procedures procedure NOUN eurjchem-1439 173 11 : : PUNCT eurjchem-1439 173 12 text text NOUN eurjchem-1439 173 13 and and CCONJ eurjchem-1439 173 14 methodology methodology NOUN eurjchem-1439 173 15 , , PUNCT eurjchem-1439 173 16 note note VERB eurjchem-1439 173 17 for for ADP eurjchem-1439 173 18 guidance guidance NOUN eurjchem-1439 173 19 on on ADP eurjchem-1439 173 20 validation validation NOUN eurjchem-1439 173 21 of of ADP eurjchem-1439 173 22 analytical analytical ADJ eurjchem-1439 173 23 procedures procedure NOUN eurjchem-1439 173 24 text text NOUN eurjchem-1439 173 25 and and CCONJ eurjchem-1439 173 26 methodology methodology NOUN eurjchem-1439 173 27 ( ( PUNCT eurjchem-1439 173 28 cpmp cpmp NOUN eurjchem-1439 173 29 / / SYM eurjchem-1439 173 30 ich/381/95 ich/381/95 NOUN eurjchem-1439 173 31 ) ) PUNCT eurjchem-1439 173 32 , , PUNCT eurjchem-1439 173 33 june june PROPN eurjchem-1439 173 34 1995 1995 NUM eurjchem-1439 173 35 . . PUNCT eurjchem-1439 174 1 [ [ X eurjchem-1439 174 2 9 9 NUM eurjchem-1439 174 3 ] ] PUNCT eurjchem-1439 174 4 . . PUNCT eurjchem-1439 175 1 varma varma PROPN eurjchem-1439 175 2 , , PUNCT eurjchem-1439 175 3 m. m. NOUN eurjchem-1439 175 4 v. v. PROPN eurjchem-1439 175 5 s. s. PROPN eurjchem-1439 175 6 ; ; PUNCT eurjchem-1439 175 7 kapoor kapoor PROPN eurjchem-1439 175 8 , , PUNCT eurjchem-1439 175 9 n. n. PROPN eurjchem-1439 175 10 ; ; PUNCT eurjchem-1439 175 11 sarkar sarkar PROPN eurjchem-1439 175 12 , , PUNCT eurjchem-1439 175 13 m. m. NOUN eurjchem-1439 175 14 ; ; PUNCT eurjchem-1439 175 15 panchagnula panchagnula PROPN eurjchem-1439 175 16 , , PUNCT eurjchem-1439 175 17 r. r. PROPN eurjchem-1439 175 18 j. j. PROPN eurjchem-1439 175 19 chromatogr chromatogr PROPN eurjchem-1439 175 20 . . PUNCT eurjchem-1439 176 1 b b PROPN eurjchem-1439 176 2 2004 2004 NUM eurjchem-1439 176 3 , , PUNCT eurjchem-1439 176 4 813 813 NUM eurjchem-1439 176 5 , , PUNCT eurjchem-1439 176 6 347‐352 347‐352 NUM eurjchem-1439 176 7 . . PUNCT eurjchem-1439 177 1 [ [ X eurjchem-1439 177 2 10 10 NUM eurjchem-1439 177 3 ] ] PUNCT eurjchem-1439 177 4 . . PUNCT eurjchem-1439 178 1 sarkar sarkar PROPN eurjchem-1439 178 2 , , PUNCT eurjchem-1439 178 3 m. m. NOUN eurjchem-1439 178 4 ; ; PUNCT eurjchem-1439 178 5 khandavilli khandavilli PROPN eurjchem-1439 178 6 , , PUNCT eurjchem-1439 178 7 s. s. PROPN eurjchem-1439 178 8 ; ; PUNCT eurjchem-1439 178 9 panchagnula panchagnula PROPN eurjchem-1439 178 10 , , PUNCT eurjchem-1439 178 11 r. r. PROPN eurjchem-1439 178 12 j. j. PROPN eurjchem-1439 178 13 chromatogr chromatogr PROPN eurjchem-1439 178 14 . . PUNCT eurjchem-1439 179 1 b b X eurjchem-1439 179 2 2006 2006 NUM eurjchem-1439 179 3 , , PUNCT eurjchem-1439 179 4 830 830 NUM eurjchem-1439 179 5 , , PUNCT eurjchem-1439 179 6 349‐354 349‐354 NUM eurjchem-1439 179 7 . . PUNCT