id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
bam-12565	Dowling, Paul; Gargan, Stephen; Zweyer, Margit; Henry, Michael; Meleady, Paula; Swandulla, Dieter; Ohlendieck, Kay	Proteomic reference map for sarcopenia research: mass spectrometric identification of key muscle proteins of organelles, cellular signaling, bioenergetic metabolism and molecular chaperoning	2024	17	.pdf	application/pdf	9396	533	50	Following a centrif- ugation step at 14,000×g for 15 min, the further processing of samples, the switching of buffers and peptide generation by trypsin digestion was carried out by the filter-aided sam- ple preparation (FASP) technique.55 Proteomic analysis For the label-free liquid chromatography mass spectromet- ric analysis of senescent hindlimb muscles from normal mice (n=6 biological repeats; n=2 technical repeats), a Thermo Orbitrap Fusion Tribrid mass spectrometer from Thermo Fisher Scientific (Waltham, MA, USA) was used.41 The detailed description of all analytical steps, including the listing of buffer composition, FASP protocol, timing of preparative stages, data-dependent acquisition, and bio- informatic data handling, used during the proteomic analy- sis of muscle proteins, has been described in a recently published methods paper.56 A Thermo UltiMate 3000 nano system was used for reversed-phase capillary high-pressure liquid chromatography and directly coupled in-line with the Thermo Orbitrap Fusion Tribrid mass spectrometer. Skeletal muscle heat shock protein 70: di- verse functions and therapeutic potential for wasting disorders.	cache/bam-12565.pdf	txt/bam-12565.txt