id sid tid token lemma pos bam-4823 1 1 bam bam VERB bam-4823 1 2 12 12 NUM bam-4823 1 3 ( ( PUNCT bam-4823 1 4 1 1 NUM bam-4823 1 5 ) ) PUNCT bam-4823 1 6 2002 2002 NUM bam-4823 1 7 skeletal skeletal ADJ bam-4823 1 8 muscle muscle NOUN bam-4823 1 9 triad triad VERB bam-4823 1 10 by by ADP bam-4823 1 11 fib fib NOUN bam-4823 1 12 milling milling NOUN bam-4823 1 13 and and CCONJ bam-4823 1 14 cryo cryo NOUN bam-4823 1 15 - - PUNCT bam-4823 1 16 et et PROPN bam-4823 1 17 eur eur PROPN bam-4823 1 18 j j PROPN bam-4823 1 19 transl transl PROPN bam-4823 1 20 myol myol PROPN bam-4823 1 21 basic basic ADJ bam-4823 1 22 appl appl NOUN bam-4823 1 23 myol myol NOUN bam-4823 1 24 2015 2015 NUM bam-4823 1 25 ; ; PUNCT bam-4823 1 26 25 25 NUM bam-4823 1 27 ( ( PUNCT bam-4823 1 28 1 1 NUM bam-4823 1 29 ): ): PUNCT bam-4823 1 30 49 49 NUM bam-4823 1 31 - - SYM bam-4823 1 32 56 56 NUM bam-4823 1 33 49 49 NUM bam-4823 1 34 skeletal skeletal ADJ bam-4823 1 35 muscle muscle NOUN bam-4823 1 36 triad triad ADJ bam-4823 1 37 junction junction NOUN bam-4823 1 38 ultrastructure ultrastructure NOUN bam-4823 1 39 by by ADP bam-4823 1 40 focused focused ADJ bam-4823 1 41 - - PUNCT bam-4823 1 42 ion ion NOUN bam-4823 1 43 - - PUNCT bam-4823 1 44 beam beam NOUN bam-4823 1 45 milling milling NOUN bam-4823 1 46 of of ADP bam-4823 1 47 muscle muscle NOUN bam-4823 1 48 and and CCONJ bam-4823 1 49 cryo cryo NOUN bam-4823 1 50 - - PUNCT bam-4823 1 51 electron electron NOUN bam-4823 1 52 tomography tomography NOUN bam-4823 1 53 terence terence PROPN bam-4823 1 54 wagenknecht wagenknecht PROPN bam-4823 1 55 ( ( PUNCT bam-4823 1 56 1,2 1,2 NUM bam-4823 1 57 ) ) PUNCT bam-4823 1 58 , , PUNCT bam-4823 1 59 chyongere chyongere PROPN bam-4823 1 60 hsieh hsieh PROPN bam-4823 1 61 ( ( PUNCT bam-4823 1 62 1 1 NUM bam-4823 1 63 ) ) PUNCT bam-4823 1 64 , , PUNCT bam-4823 1 65 michael michael PROPN bam-4823 1 66 marko marko PROPN bam-4823 1 67 ( ( PUNCT bam-4823 1 68 1 1 NUM bam-4823 1 69 ) ) PUNCT bam-4823 1 70 ( ( PUNCT bam-4823 1 71 1 1 X bam-4823 1 72 ) ) PUNCT bam-4823 1 73 new new PROPN bam-4823 1 74 york york PROPN bam-4823 1 75 state state PROPN bam-4823 1 76 department department PROPN bam-4823 1 77 of of ADP bam-4823 1 78 health health PROPN bam-4823 1 79 , , PUNCT bam-4823 1 80 wadsworth wadsworth NOUN bam-4823 1 81 cente cente NOUN bam-4823 1 82 , , PUNCT bam-4823 1 83 empire empire NOUN bam-4823 1 84 state state PROPN bam-4823 1 85 plaza plaza PROPN bam-4823 1 86 , , PUNCT bam-4823 1 87 albany albany PROPN bam-4823 1 88 , , PUNCT bam-4823 1 89 ny ny PROPN bam-4823 1 90 ; ; PUNCT bam-4823 1 91 ( ( PUNCT bam-4823 1 92 2 2 X bam-4823 1 93 ) ) PUNCT bam-4823 1 94 department department NOUN bam-4823 1 95 of of ADP bam-4823 1 96 biomedical biomedical ADJ bam-4823 1 97 sciences science NOUN bam-4823 1 98 , , PUNCT bam-4823 1 99 school school NOUN bam-4823 1 100 of of ADP bam-4823 1 101 public public ADJ bam-4823 1 102 health health NOUN bam-4823 1 103 , , PUNCT bam-4823 1 104 university university NOUN bam-4823 1 105 at at ADP bam-4823 1 106 albany albany PROPN bam-4823 1 107 , , PUNCT bam-4823 1 108 albany albany PROPN bam-4823 1 109 , , PUNCT bam-4823 1 110 ny ny PROPN bam-4823 1 111 , , PUNCT bam-4823 1 112 usa usa PROPN bam-4823 1 113 abstract abstract PROPN bam-4823 1 114 cryo cryo PROPN bam-4823 1 115 - - PUNCT bam-4823 1 116 electron electron NOUN bam-4823 1 117 tomography tomography NOUN bam-4823 1 118 ( ( PUNCT bam-4823 1 119 cryo cryo NOUN bam-4823 1 120 - - PUNCT bam-4823 1 121 et et NOUN bam-4823 1 122 ) ) PUNCT bam-4823 1 123 has have AUX bam-4823 1 124 emerged emerge VERB bam-4823 1 125 as as ADP bam-4823 1 126 perhaps perhaps ADV bam-4823 1 127 the the DET bam-4823 1 128 only only ADJ bam-4823 1 129 practical practical ADJ bam-4823 1 130 technique technique NOUN bam-4823 1 131 for for ADP bam-4823 1 132 revealing reveal VERB bam-4823 1 133 nanometer nanometer NOUN bam-4823 1 134 - - PUNCT bam-4823 1 135 level level NOUN bam-4823 1 136 three three NUM bam-4823 1 137 - - PUNCT bam-4823 1 138 dimensional dimensional ADJ bam-4823 1 139 structural structural ADJ bam-4823 1 140 details detail NOUN bam-4823 1 141 of of ADP bam-4823 1 142 subcellular subcellular ADJ bam-4823 1 143 macromolecular macromolecular NOUN bam-4823 1 144 complexes complex NOUN bam-4823 1 145 in in ADP bam-4823 1 146 their their PRON bam-4823 1 147 native native ADJ bam-4823 1 148 context context NOUN bam-4823 1 149 , , PUNCT bam-4823 1 150 inside inside ADP bam-4823 1 151 the the DET bam-4823 1 152 cell cell NOUN bam-4823 1 153 . . PUNCT bam-4823 2 1 as as SCONJ bam-4823 2 2 currently currently ADV bam-4823 2 3 practiced practice VERB bam-4823 2 4 , , PUNCT bam-4823 2 5 the the DET bam-4823 2 6 specimen speciman NOUN bam-4823 2 7 should should AUX bam-4823 2 8 be be AUX bam-4823 2 9 0.10.2 0.10.2 NUM bam-4823 2 10 microns micron NOUN bam-4823 2 11 in in ADP bam-4823 2 12 thickness thickness NOUN bam-4823 2 13 to to PART bam-4823 2 14 achieve achieve VERB bam-4823 2 15 optimal optimal ADJ bam-4823 2 16 resolution resolution NOUN bam-4823 2 17 . . PUNCT bam-4823 3 1 thus thus ADV bam-4823 3 2 , , PUNCT bam-4823 3 3 application application NOUN bam-4823 3 4 of of ADP bam-4823 3 5 cryo cryo NOUN bam-4823 3 6 - - PUNCT bam-4823 3 7 et et NOUN bam-4823 3 8 to to ADP bam-4823 3 9 intact intact ADJ bam-4823 3 10 frozen frozen ADJ bam-4823 3 11 ( ( PUNCT bam-4823 3 12 vitreous vitreous ADJ bam-4823 3 13 ) ) PUNCT bam-4823 3 14 tissues tissue NOUN bam-4823 3 15 , , PUNCT bam-4823 3 16 such such ADJ bam-4823 3 17 as as ADP bam-4823 3 18 skeletal skeletal ADJ bam-4823 3 19 muscle muscle NOUN bam-4823 3 20 , , PUNCT bam-4823 3 21 requires require VERB bam-4823 3 22 that that SCONJ bam-4823 3 23 they they PRON bam-4823 3 24 be be AUX bam-4823 3 25 sectioned section VERB bam-4823 3 26 . . PUNCT bam-4823 4 1 cryoultramicrotomy cryoultramicrotomy NOUN bam-4823 4 2 is be AUX bam-4823 4 3 notoriously notoriously ADV bam-4823 4 4 difficult difficult ADJ bam-4823 4 5 and and CCONJ bam-4823 4 6 artifact artifact ADJ bam-4823 4 7 - - PUNCT bam-4823 4 8 prone prone ADJ bam-4823 4 9 when when SCONJ bam-4823 4 10 applied apply VERB bam-4823 4 11 to to ADP bam-4823 4 12 frozen frozen ADJ bam-4823 4 13 cells cell NOUN bam-4823 4 14 and and CCONJ bam-4823 4 15 tissue tissue NOUN bam-4823 4 16 , , PUNCT bam-4823 4 17 but but CCONJ bam-4823 4 18 a a DET bam-4823 4 19 new new ADJ bam-4823 4 20 technique technique NOUN bam-4823 4 21 , , PUNCT bam-4823 4 22 focused focused ADJ bam-4823 4 23 ion ion NOUN bam-4823 4 24 beam beam NOUN bam-4823 4 25 milling milling NOUN bam-4823 4 26 ( ( PUNCT bam-4823 4 27 cryo cryo NOUN bam-4823 4 28 - - PUNCT bam-4823 4 29 fib fib NOUN bam-4823 4 30 ) ) PUNCT bam-4823 4 31 , , PUNCT bam-4823 4 32 shows show VERB bam-4823 4 33 great great ADJ bam-4823 4 34 promise promise NOUN bam-4823 4 35 for for ADP bam-4823 4 36 “ " PUNCT bam-4823 4 37 thinning thin VERB bam-4823 4 38 ” " PUNCT bam-4823 4 39 frozen frozen ADJ bam-4823 4 40 biological biological ADJ bam-4823 4 41 specimens specimen NOUN bam-4823 4 42 . . PUNCT bam-4823 5 1 here here ADV bam-4823 5 2 we we PRON bam-4823 5 3 describe describe VERB bam-4823 5 4 our our PRON bam-4823 5 5 initial initial ADJ bam-4823 5 6 results result NOUN bam-4823 5 7 in in ADP bam-4823 5 8 applying apply VERB bam-4823 5 9 cryofib cryofib ADJ bam-4823 5 10 and and CCONJ bam-4823 5 11 cryo cryo NOUN bam-4823 5 12 - - PUNCT bam-4823 5 13 et et NOUN bam-4823 5 14 to to ADP bam-4823 5 15 triad triad VERB bam-4823 5 16 junctions junction NOUN bam-4823 5 17 of of ADP bam-4823 5 18 skeletal skeletal ADJ bam-4823 5 19 muscle muscle NOUN bam-4823 5 20 . . PUNCT bam-4823 6 1 key key ADJ bam-4823 6 2 words word NOUN bam-4823 6 3 : : PUNCT bam-4823 6 4 cryo cryo NOUN bam-4823 6 5 - - PUNCT bam-4823 6 6 electron electron NOUN bam-4823 6 7 microscopy microscopy NOUN bam-4823 6 8 , , PUNCT bam-4823 6 9 cryo cryo NOUN bam-4823 6 10 - - PUNCT bam-4823 6 11 electron electron NOUN bam-4823 6 12 tomography tomography NOUN bam-4823 6 13 , , PUNCT bam-4823 6 14 excitation excitation NOUN bam-4823 6 15 - - PUNCT bam-4823 6 16 contraction contraction NOUN bam-4823 6 17 coupling coupling NOUN bam-4823 6 18 , , PUNCT bam-4823 6 19 focused focused ADJ bam-4823 6 20 - - PUNCT bam-4823 6 21 ion ion NOUN bam-4823 6 22 - - PUNCT bam-4823 6 23 beam beam NOUN bam-4823 6 24 milling milling NOUN bam-4823 6 25 , , PUNCT bam-4823 6 26 skeletal skeletal ADJ bam-4823 6 27 muscle muscle NOUN bam-4823 6 28 eur eur PROPN bam-4823 6 29 j j PROPN bam-4823 6 30 transl transl PROPN bam-4823 6 31 myol myol PROPN bam-4823 6 32 basic basic ADJ bam-4823 6 33 appl appl NOUN bam-4823 6 34 myol myol NOUN bam-4823 6 35 2015 2015 NUM bam-4823 6 36 ; ; PUNCT bam-4823 6 37 25 25 NUM bam-4823 6 38 ( ( PUNCT bam-4823 6 39 1 1 NUM bam-4823 6 40 ): ): PUNCT bam-4823 6 41 49 49 NUM bam-4823 6 42 - - SYM bam-4823 6 43 56 56 NUM bam-4823 6 44 current current ADJ bam-4823 6 45 understanding understanding NOUN bam-4823 6 46 of of ADP bam-4823 6 47 the the DET bam-4823 6 48 molecular molecular ADJ bam-4823 6 49 structure structure NOUN bam-4823 6 50 of of ADP bam-4823 6 51 the the DET bam-4823 6 52 triad triad ADJ bam-4823 6 53 junction junction NOUN bam-4823 6 54 , , PUNCT bam-4823 6 55 the the DET bam-4823 6 56 site site NOUN bam-4823 6 57 of of ADP bam-4823 6 58 excitation excitation NOUN bam-4823 6 59 - - PUNCT bam-4823 6 60 contraction contraction NOUN bam-4823 6 61 in in ADP bam-4823 6 62 skeletal skeletal ADJ bam-4823 6 63 muscle muscle NOUN bam-4823 6 64 , , PUNCT bam-4823 6 65 is be AUX bam-4823 6 66 severely severely ADV bam-4823 6 67 limited limited ADJ bam-4823 6 68 . . PUNCT bam-4823 7 1 most most ADJ bam-4823 7 2 knowledge knowledge NOUN bam-4823 7 3 of of ADP bam-4823 7 4 overall overall ADJ bam-4823 7 5 triad triad ADJ bam-4823 7 6 junction junction NOUN bam-4823 7 7 architecture architecture NOUN bam-4823 7 8 has have AUX bam-4823 7 9 come come VERB bam-4823 7 10 from from ADP bam-4823 7 11 classical classical ADJ bam-4823 7 12 transmission transmission NOUN bam-4823 7 13 electron electron NOUN bam-4823 7 14 microscopy microscopy NOUN bam-4823 7 15 ( ( PUNCT bam-4823 7 16 tem tem PROPN bam-4823 7 17 ) ) PUNCT bam-4823 7 18 of of ADP bam-4823 7 19 sectioned sectioned NOUN bam-4823 7 20 or or CCONJ bam-4823 7 21 freeze freeze NOUN bam-4823 7 22 - - PUNCT bam-4823 7 23 fractured fractured ADJ bam-4823 7 24 muscle muscle NOUN bam-4823 7 25 1 1 NUM bam-4823 7 26 . . PUNCT bam-4823 8 1 to to ADP bam-4823 8 2 date date NOUN bam-4823 8 3 it it PRON bam-4823 8 4 has have AUX bam-4823 8 5 not not PART bam-4823 8 6 been be AUX bam-4823 8 7 possible possible ADJ bam-4823 8 8 to to PART bam-4823 8 9 purify purify VERB bam-4823 8 10 triad triad ADJ bam-4823 8 11 junctions junction NOUN bam-4823 8 12 , , PUNCT bam-4823 8 13 or or CCONJ bam-4823 8 14 natively natively ADV bam-4823 8 15 preserved preserve VERB bam-4823 8 16 fragments fragment NOUN bam-4823 8 17 thereof thereof ADV bam-4823 8 18 , , PUNCT bam-4823 8 19 for for ADP bam-4823 8 20 more more ADV bam-4823 8 21 detailed detailed ADJ bam-4823 8 22 study study NOUN bam-4823 8 23 , , PUNCT bam-4823 8 24 although although SCONJ bam-4823 8 25 microsomal microsomal ADJ bam-4823 8 26 fractions fraction NOUN bam-4823 8 27 that that PRON bam-4823 8 28 retain retain VERB bam-4823 8 29 some some DET bam-4823 8 30 limited limited ADJ bam-4823 8 31 similarities similarity NOUN bam-4823 8 32 to to ADP bam-4823 8 33 native native ADJ bam-4823 8 34 triad triad ADJ bam-4823 8 35 junctions junction NOUN bam-4823 8 36 have have AUX bam-4823 8 37 been be AUX bam-4823 8 38 isolated isolate VERB bam-4823 8 39 and and CCONJ bam-4823 8 40 characterized characterize VERB bam-4823 8 41 by by ADP bam-4823 8 42 tem tem PROPN bam-4823 8 43 cryo cryo PROPN bam-4823 8 44 - - PUNCT bam-4823 8 45 tomography tomography NOUN bam-4823 8 46 ( ( PUNCT bam-4823 8 47 cryoet cryoet ADJ bam-4823 8 48 ) ) PUNCT bam-4823 8 49 . . PUNCT bam-4823 9 1 2 2 NUM bam-4823 9 2 the the DET bam-4823 9 3 two two NUM bam-4823 9 4 major major ADJ bam-4823 9 5 membrane membrane NOUN bam-4823 9 6 - - PUNCT bam-4823 9 7 associated associate VERB bam-4823 9 8 components component NOUN bam-4823 9 9 of of ADP bam-4823 9 10 the the DET bam-4823 9 11 triad triad NOUN bam-4823 9 12 , , PUNCT bam-4823 9 13 the the DET bam-4823 9 14 ryanodine ryanodine NOUN bam-4823 9 15 receptor/ receptor/ NUM bam-4823 9 16 calcium calcium NOUN bam-4823 9 17 release release NOUN bam-4823 9 18 channel channel NOUN bam-4823 9 19 and and CCONJ bam-4823 9 20 the the DET bam-4823 9 21 dihydropyridine dihydropyridine NOUN bam-4823 9 22 receptor receptor NOUN bam-4823 9 23 / / SYM bam-4823 9 24 voltage voltage NOUN bam-4823 9 25 sensor sensor NOUN bam-4823 9 26 , , PUNCT bam-4823 9 27 have have AUX bam-4823 9 28 been be AUX bam-4823 9 29 isolated isolate VERB bam-4823 9 30 and and CCONJ bam-4823 9 31 studied study VERB bam-4823 9 32 by by ADP bam-4823 9 33 single single ADJ bam-4823 9 34 - - PUNCT bam-4823 9 35 particle particle NOUN bam-4823 9 36 cryo cryo NOUN bam-4823 9 37 - - PUNCT bam-4823 9 38 em em PRON bam-4823 9 39 techniques technique NOUN bam-4823 9 40 ( ( PUNCT bam-4823 9 41 reviewed review VERB bam-4823 9 42 by by ADP bam-4823 9 43 hamilton hamilton PROPN bam-4823 9 44 et et PROPN bam-4823 9 45 al al PROPN bam-4823 9 46 . . PROPN bam-4823 9 47 3 3 NUM bam-4823 9 48 and and CCONJ bam-4823 9 49 by by ADP bam-4823 9 50 wong wong PROPN bam-4823 9 51 et et PROPN bam-4823 9 52 al al PROPN bam-4823 9 53 . . PROPN bam-4823 9 54 4 4 NUM bam-4823 9 55 ) ) PUNCT bam-4823 9 56 but but CCONJ bam-4823 9 57 little little ADJ bam-4823 9 58 , , PUNCT bam-4823 9 59 if if SCONJ bam-4823 9 60 any any PRON bam-4823 9 61 , , PUNCT bam-4823 9 62 success success NOUN bam-4823 9 63 in in ADP bam-4823 9 64 reconstituting reconstitute VERB bam-4823 9 65 these these PRON bam-4823 9 66 and and CCONJ bam-4823 9 67 other other ADJ bam-4823 9 68 triad triad ADJ bam-4823 9 69 components component NOUN bam-4823 9 70 has have AUX bam-4823 9 71 been be AUX bam-4823 9 72 reported report VERB bam-4823 9 73 to to ADP bam-4823 9 74 date date NOUN bam-4823 9 75 . . PUNCT bam-4823 10 1 recent recent ADJ bam-4823 10 2 applications application NOUN bam-4823 10 3 of of ADP bam-4823 10 4 cryo cryo NOUN bam-4823 10 5 - - PUNCT bam-4823 10 6 et et NOUN bam-4823 10 7 to to AUX bam-4823 10 8 intact intact ADJ bam-4823 10 9 bacterial bacterial ADJ bam-4823 10 10 and and CCONJ bam-4823 10 11 eukaryotic eukaryotic ADJ bam-4823 10 12 cells cell NOUN bam-4823 10 13 have have AUX bam-4823 10 14 shown show VERB bam-4823 10 15 that that SCONJ bam-4823 10 16 large large ADJ bam-4823 10 17 macromolecular macromolecular NOUN bam-4823 10 18 complexes complex NOUN bam-4823 10 19 ( ( PUNCT bam-4823 10 20 e.g. e.g. ADV bam-4823 10 21 ribosomes ribosome NOUN bam-4823 10 22 , , PUNCT bam-4823 10 23 nuclear nuclear ADJ bam-4823 10 24 pores pore NOUN bam-4823 10 25 ) ) PUNCT bam-4823 10 26 , , PUNCT bam-4823 10 27 including include VERB bam-4823 10 28 membrane membrane NOUN bam-4823 10 29 - - PUNCT bam-4823 10 30 associated associate VERB bam-4823 10 31 ones one NOUN bam-4823 10 32 , , PUNCT bam-4823 10 33 can can AUX bam-4823 10 34 be be AUX bam-4823 10 35 resolved resolve VERB bam-4823 10 36 and and CCONJ bam-4823 10 37 , , PUNCT bam-4823 10 38 if if SCONJ bam-4823 10 39 multiple multiple ADJ bam-4823 10 40 copies copy NOUN bam-4823 10 41 are be AUX bam-4823 10 42 present present ADJ bam-4823 10 43 , , PUNCT bam-4823 10 44 the the DET bam-4823 10 45 individual individual ADJ bam-4823 10 46 subvolumes subvolume NOUN bam-4823 10 47 containing contain VERB bam-4823 10 48 them they PRON bam-4823 10 49 can can AUX bam-4823 10 50 be be AUX bam-4823 10 51 computationally computationally ADV bam-4823 10 52 selected select VERB bam-4823 10 53 and and CCONJ bam-4823 10 54 averaged average VERB bam-4823 10 55 to to PART bam-4823 10 56 improve improve VERB bam-4823 10 57 the the DET bam-4823 10 58 resolution resolution NOUN bam-4823 10 59 ( ( PUNCT bam-4823 10 60 in in ADP bam-4823 10 61 favorable favorable ADJ bam-4823 10 62 cases case NOUN bam-4823 10 63 ) ) PUNCT bam-4823 10 64 to to ADP bam-4823 10 65 2 2 NUM bam-4823 10 66 nm nm NOUN bam-4823 10 67 ( ( PUNCT bam-4823 10 68 recently recently ADV bam-4823 10 69 reviewed review VERB bam-4823 10 70 ) ) PUNCT bam-4823 10 71 . . PUNCT bam-4823 11 1 5,6 5,6 NUM bam-4823 11 2 significant significant ADJ bam-4823 11 3 improvements improvement NOUN bam-4823 11 4 in in ADP bam-4823 11 5 resolution resolution NOUN bam-4823 11 6 are be AUX bam-4823 11 7 likely likely ADV bam-4823 11 8 forthcoming forthcoming ADJ bam-4823 11 9 ( ( PUNCT bam-4823 11 10 see see VERB bam-4823 11 11 “ " PUNCT bam-4823 11 12 outlook outlook NOUN bam-4823 11 13 , , PUNCT bam-4823 11 14 ” " PUNCT bam-4823 11 15 below below ADP bam-4823 11 16 ) ) PUNCT bam-4823 11 17 . . PUNCT bam-4823 12 1 cryo cryo PROPN bam-4823 12 2 - - PUNCT bam-4823 12 3 et et PROPN bam-4823 12 4 allows allow VERB bam-4823 12 5 subcellular subcellular ADJ bam-4823 12 6 structures structure NOUN bam-4823 12 7 to to PART bam-4823 12 8 be be AUX bam-4823 12 9 imaged image VERB bam-4823 12 10 in in ADP bam-4823 12 11 situ situ NOUN bam-4823 12 12 in in ADP bam-4823 12 13 their their PRON bam-4823 12 14 native native ADJ bam-4823 12 15 state state NOUN bam-4823 12 16 because because SCONJ bam-4823 12 17 no no DET bam-4823 12 18 fixatives fixative NOUN bam-4823 12 19 and and CCONJ bam-4823 12 20 no no DET bam-4823 12 21 heavy heavy ADJ bam-4823 12 22 - - PUNCT bam-4823 12 23 metal metal NOUN bam-4823 12 24 stains stain NOUN bam-4823 12 25 are be AUX bam-4823 12 26 required require VERB bam-4823 12 27 prior prior ADV bam-4823 12 28 to to ADP bam-4823 12 29 freezing freeze VERB bam-4823 12 30 the the DET bam-4823 12 31 specimen speciman NOUN bam-4823 12 32 , , PUNCT bam-4823 12 33 which which PRON bam-4823 12 34 is be AUX bam-4823 12 35 done do VERB bam-4823 12 36 at at ADP bam-4823 12 37 a a DET bam-4823 12 38 sufficiently sufficiently ADV bam-4823 12 39 high high ADJ bam-4823 12 40 rate rate NOUN bam-4823 12 41 ( ( PUNCT bam-4823 12 42 or or CCONJ bam-4823 12 43 pressure pressure NOUN bam-4823 12 44 ) ) PUNCT bam-4823 12 45 that that SCONJ bam-4823 12 46 crystalline crystalline ADJ bam-4823 12 47 ice ice NOUN bam-4823 12 48 does do AUX bam-4823 12 49 not not PART bam-4823 12 50 form form VERB bam-4823 12 51 . . PUNCT bam-4823 13 1 the the DET bam-4823 13 2 resulting result VERB bam-4823 13 3 “ " PUNCT bam-4823 13 4 vitreous vitreous ADJ bam-4823 13 5 ” " PUNCT bam-4823 13 6 ice ice NOUN bam-4823 13 7 is be AUX bam-4823 13 8 known know VERB bam-4823 13 9 to to PART bam-4823 13 10 allow allow VERB bam-4823 13 11 macromolecular macromolecular ADJ bam-4823 13 12 preservation preservation NOUN bam-4823 13 13 to to ADP bam-4823 13 14 atomic atomic ADJ bam-4823 13 15 levels level NOUN bam-4823 13 16 . . PUNCT bam-4823 14 1 the the DET bam-4823 14 2 contrast contrast NOUN bam-4823 14 3 of of ADP bam-4823 14 4 the the DET bam-4823 14 5 tomograms tomogram NOUN bam-4823 14 6 computed compute VERB bam-4823 14 7 from from ADP bam-4823 14 8 the the DET bam-4823 14 9 micrographs micrograph NOUN bam-4823 14 10 in in ADP bam-4823 14 11 the the DET bam-4823 14 12 tilt tilt NOUN bam-4823 14 13 series series NOUN bam-4823 14 14 is be AUX bam-4823 14 15 very very ADV bam-4823 14 16 low low ADJ bam-4823 14 17 , , PUNCT bam-4823 14 18 such such ADJ bam-4823 14 19 that that SCONJ bam-4823 14 20 only only ADV bam-4823 14 21 membrane membrane NOUN bam-4823 14 22 - - PUNCT bam-4823 14 23 bounded bound VERB bam-4823 14 24 organelles organelle NOUN bam-4823 14 25 and and CCONJ bam-4823 14 26 large large ADJ bam-4823 14 27 macromolecular macromolecular NOUN bam-4823 14 28 complexes complex NOUN bam-4823 14 29 are be AUX bam-4823 14 30 readily readily ADV bam-4823 14 31 visualized visualize VERB bam-4823 14 32 , , PUNCT bam-4823 14 33 but but CCONJ bam-4823 14 34 cryo cryo NOUN bam-4823 14 35 - - PUNCT bam-4823 14 36 imaging image VERB bam-4823 14 37 technology technology NOUN bam-4823 14 38 is be AUX bam-4823 14 39 constantly constantly ADV bam-4823 14 40 improving improve VERB bam-4823 14 41 , , PUNCT bam-4823 14 42 and and CCONJ bam-4823 14 43 additional additional ADJ bam-4823 14 44 image image NOUN bam-4823 14 45 processing processing NOUN bam-4823 14 46 , , PUNCT bam-4823 14 47 such such ADJ bam-4823 14 48 as as ADP bam-4823 14 49 subtomogram subtomogram NOUN bam-4823 14 50 averaging averaging NOUN bam-4823 14 51 , , PUNCT bam-4823 14 52 where where SCONJ bam-4823 14 53 this this PRON bam-4823 14 54 is be AUX bam-4823 14 55 possible possible ADJ bam-4823 14 56 , , PUNCT bam-4823 14 57 can can AUX bam-4823 14 58 significantly significantly ADV bam-4823 14 59 improve improve VERB bam-4823 14 60 the the DET bam-4823 14 61 attainable attainable ADJ bam-4823 14 62 structural structural ADJ bam-4823 14 63 information information NOUN bam-4823 14 64 . . PUNCT bam-4823 15 1 a a DET bam-4823 15 2 major major ADJ bam-4823 15 3 obstacle obstacle NOUN bam-4823 15 4 to to ADP bam-4823 15 5 applying apply VERB bam-4823 15 6 cryo cryo NOUN bam-4823 15 7 - - PUNCT bam-4823 15 8 et et NOUN bam-4823 15 9 to to ADP bam-4823 15 10 large large ADJ bam-4823 15 11 eukaryotic eukaryotic ADJ bam-4823 15 12 cells cell NOUN bam-4823 15 13 and and CCONJ bam-4823 15 14 tissues tissue NOUN bam-4823 15 15 , , PUNCT bam-4823 15 16 such such ADJ bam-4823 15 17 as as ADP bam-4823 15 18 muscle muscle NOUN bam-4823 15 19 , , PUNCT bam-4823 15 20 has have AUX bam-4823 15 21 been be AUX bam-4823 15 22 that that PRON bam-4823 15 23 for for ADP bam-4823 15 24 these these DET bam-4823 15 25 specimens specimen NOUN bam-4823 15 26 it it PRON bam-4823 15 27 is be AUX bam-4823 15 28 necessary necessary ADJ bam-4823 15 29 to to PART bam-4823 15 30 use use VERB bam-4823 15 31 highpressure highpressure ADJ bam-4823 15 32 freezing freezing NOUN bam-4823 15 33 to to PART bam-4823 15 34 achieve achieve VERB bam-4823 15 35 the the DET bam-4823 15 36 vitreous vitreous ADJ bam-4823 15 37 form form NOUN bam-4823 15 38 of of ADP bam-4823 15 39 ice ice NOUN bam-4823 15 40 . . PUNCT bam-4823 16 1 it it PRON bam-4823 16 2 is be AUX bam-4823 16 3 then then ADV bam-4823 16 4 necessary necessary ADJ bam-4823 16 5 to to ADP bam-4823 16 6 “ " PUNCT bam-4823 16 7 thin thin ADJ bam-4823 16 8 ” " PUNCT bam-4823 16 9 the the DET bam-4823 16 10 specimen speciman NOUN bam-4823 16 11 to to ADP bam-4823 16 12 less less ADJ bam-4823 16 13 than than ADP bam-4823 16 14 500 500 NUM bam-4823 16 15 nm nm NOUN bam-4823 16 16 ( ( PUNCT bam-4823 16 17 100 100 NUM bam-4823 16 18 - - SYM bam-4823 16 19 200 200 NUM bam-4823 16 20 nm nm NOUN bam-4823 16 21 is be AUX bam-4823 16 22 preferable preferable ADJ bam-4823 16 23 for for ADP bam-4823 16 24 achieving achieve VERB bam-4823 16 25 highest high ADJ bam-4823 16 26 resolution resolution NOUN bam-4823 16 27 ) ) PUNCT bam-4823 16 28 and and CCONJ bam-4823 16 29 then then ADV bam-4823 16 30 transfer transfer VERB bam-4823 16 31 the the DET bam-4823 16 32 sample sample NOUN bam-4823 16 33 to to ADP bam-4823 16 34 a a DET bam-4823 16 35 specimen speciman NOUN bam-4823 16 36 holder holder NOUN bam-4823 16 37 for for ADP bam-4823 16 38 insertion insertion NOUN bam-4823 16 39 into into ADP bam-4823 16 40 the the DET bam-4823 16 41 tem tem NOUN bam-4823 16 42 , , PUNCT bam-4823 16 43 all all DET bam-4823 16 44 the the DET bam-4823 16 45 while while NOUN bam-4823 16 46 maintaining maintain VERB bam-4823 16 47 low low ADJ bam-4823 16 48 temperature temperature NOUN bam-4823 16 49 and and CCONJ bam-4823 16 50 avoiding avoid VERB bam-4823 16 51 frost frost NOUN bam-4823 16 52 contamination contamination NOUN bam-4823 16 53 . . PUNCT bam-4823 17 1 until until ADP bam-4823 17 2 recently recently ADV bam-4823 17 3 , , PUNCT bam-4823 17 4 the the DET bam-4823 17 5 only only ADJ bam-4823 17 6 way way NOUN bam-4823 17 7 to to PART bam-4823 17 8 achieve achieve VERB bam-4823 17 9 skeletal skeletal ADJ bam-4823 17 10 muscle muscle NOUN bam-4823 17 11 triad triad VERB bam-4823 17 12 by by ADP bam-4823 17 13 fib fib NOUN bam-4823 17 14 milling milling NOUN bam-4823 17 15 and and CCONJ bam-4823 17 16 cryo cryo NOUN bam-4823 17 17 - - PUNCT bam-4823 17 18 et et PROPN bam-4823 17 19 eur eur PROPN bam-4823 17 20 j j PROPN bam-4823 17 21 transl transl PROPN bam-4823 17 22 myol myol PROPN bam-4823 17 23 basic basic ADJ bam-4823 17 24 appl appl NOUN bam-4823 17 25 myol myol NOUN bam-4823 17 26 2015 2015 NUM bam-4823 17 27 ; ; PUNCT bam-4823 17 28 25 25 NUM bam-4823 17 29 ( ( PUNCT bam-4823 17 30 1 1 NUM bam-4823 17 31 ): ): PUNCT bam-4823 17 32 49 49 NUM bam-4823 17 33 - - SYM bam-4823 17 34 56 56 NUM bam-4823 17 35 50 50 NUM bam-4823 17 36 these these DET bam-4823 17 37 steps step NOUN bam-4823 17 38 was be AUX bam-4823 17 39 to to PART bam-4823 17 40 transfer transfer VERB bam-4823 17 41 the the DET bam-4823 17 42 block block NOUN bam-4823 17 43 of of ADP bam-4823 17 44 frozen frozen ADJ bam-4823 17 45 tissue tissue NOUN bam-4823 17 46 or or CCONJ bam-4823 17 47 cell cell NOUN bam-4823 17 48 suspension suspension NOUN bam-4823 17 49 from from ADP bam-4823 17 50 the the DET bam-4823 17 51 high high ADJ bam-4823 17 52 - - PUNCT bam-4823 17 53 pressure pressure NOUN bam-4823 17 54 freezer freezer NOUN bam-4823 17 55 to to ADP bam-4823 17 56 a a DET bam-4823 17 57 cryo cryo NOUN bam-4823 17 58 - - PUNCT bam-4823 17 59 ultramicrotome ultramicrotome NOUN bam-4823 17 60 , , PUNCT bam-4823 17 61 cut cut VERB bam-4823 17 62 vitreous vitreous ADJ bam-4823 17 63 cryo cryo NOUN bam-4823 17 64 - - PUNCT bam-4823 17 65 sections section NOUN bam-4823 17 66 ( ( PUNCT bam-4823 17 67 typically typically ADV bam-4823 17 68 100 100 NUM bam-4823 17 69 - - SYM bam-4823 17 70 200 200 NUM bam-4823 17 71 nm nm ADJ bam-4823 17 72 thickness thickness NOUN bam-4823 17 73 ) ) PUNCT bam-4823 17 74 , , PUNCT bam-4823 17 75 transfer transfer VERB bam-4823 17 76 the the DET bam-4823 17 77 sections section NOUN bam-4823 17 78 to to ADP bam-4823 17 79 em em PRON bam-4823 17 80 grids grid NOUN bam-4823 17 81 , , PUNCT bam-4823 17 82 and and CCONJ bam-4823 17 83 then then ADV bam-4823 17 84 transfer transfer VERB bam-4823 17 85 the the DET bam-4823 17 86 grids grid NOUN bam-4823 17 87 to to ADP bam-4823 17 88 a a DET bam-4823 17 89 tem tem PROPN bam-4823 17 90 cryoholder cryoholder NOUN bam-4823 17 91 . . PUNCT bam-4823 18 1 technology technology NOUN bam-4823 18 2 for for ADP bam-4823 18 3 cryo cryo NOUN bam-4823 18 4 - - PUNCT bam-4823 18 5 ultramicrotomy ultramicrotomy NOUN bam-4823 18 6 has have AUX bam-4823 18 7 been be AUX bam-4823 18 8 available available ADJ bam-4823 18 9 for for ADP bam-4823 18 10 several several ADJ bam-4823 18 11 decades decade NOUN bam-4823 18 12 , , PUNCT bam-4823 18 13 but but CCONJ bam-4823 18 14 despite despite SCONJ bam-4823 18 15 improvements improvement NOUN bam-4823 18 16 7 7 NUM bam-4823 18 17 - - SYM bam-4823 18 18 12 12 NUM bam-4823 18 19 vitreous vitreous ADJ bam-4823 18 20 cryo cryo NOUN bam-4823 18 21 - - PUNCT bam-4823 18 22 sections section NOUN bam-4823 18 23 suffer suffer VERB bam-4823 18 24 from from ADP bam-4823 18 25 artifacts artifact NOUN bam-4823 18 26 such such ADJ bam-4823 18 27 as as ADP bam-4823 18 28 compression compression NOUN bam-4823 18 29 in in ADP bam-4823 18 30 the the DET bam-4823 18 31 direction direction NOUN bam-4823 18 32 of of ADP bam-4823 18 33 cutting cutting NOUN bam-4823 18 34 ( ( PUNCT bam-4823 18 35 up up ADP bam-4823 18 36 to to PART bam-4823 18 37 50 50 NUM bam-4823 18 38 % % NOUN bam-4823 18 39 ) ) PUNCT bam-4823 18 40 , , PUNCT bam-4823 18 41 crevasses crevasse NOUN bam-4823 18 42 and and CCONJ bam-4823 18 43 knife knife NOUN bam-4823 18 44 marks mark NOUN bam-4823 18 45 , , PUNCT bam-4823 18 46 all all PRON bam-4823 18 47 of of ADP bam-4823 18 48 which which PRON bam-4823 18 49 adversely adversely ADV bam-4823 18 50 affect affect VERB bam-4823 18 51 the the DET bam-4823 18 52 images image NOUN bam-4823 18 53 . . PUNCT bam-4823 19 1 10 10 NUM bam-4823 19 2 even even ADV bam-4823 19 3 more more ADV bam-4823 19 4 vexing vexing ADJ bam-4823 19 5 for for ADP bam-4823 19 6 cryo cryo NOUN bam-4823 19 7 - - PUNCT bam-4823 19 8 et et NOUN bam-4823 19 9 applications application NOUN bam-4823 19 10 , , PUNCT bam-4823 19 11 the the DET bam-4823 19 12 cryo cryo NOUN bam-4823 19 13 - - PUNCT bam-4823 19 14 sections section NOUN bam-4823 19 15 are be AUX bam-4823 19 16 almost almost ADV bam-4823 19 17 invariably invariably ADV bam-4823 19 18 non non ADJ bam-4823 19 19 - - ADJ bam-4823 19 20 planar planar ADJ bam-4823 19 21 ( ( PUNCT bam-4823 19 22 wrinkled wrinkle VERB bam-4823 19 23 ) ) PUNCT bam-4823 19 24 and and CCONJ bam-4823 19 25 difficult difficult ADJ bam-4823 19 26 to to PART bam-4823 19 27 attach attach VERB bam-4823 19 28 to to ADP bam-4823 19 29 em em PRON bam-4823 19 30 grids grid NOUN bam-4823 19 31 with with ADP bam-4823 19 32 sufficient sufficient ADJ bam-4823 19 33 stability stability NOUN bam-4823 19 34 to to PART bam-4823 19 35 allow allow VERB bam-4823 19 36 collection collection NOUN bam-4823 19 37 of of ADP bam-4823 19 38 a a DET bam-4823 19 39 suitable suitable ADJ bam-4823 19 40 tilt tilt NOUN bam-4823 19 41 series series NOUN bam-4823 19 42 of of ADP bam-4823 19 43 micrographs micrograph NOUN bam-4823 19 44 ( ( PUNCT bam-4823 19 45 typically typically ADV bam-4823 19 46 from from ADP bam-4823 19 47 60 60 NUM bam-4823 19 48 to to ADP bam-4823 19 49 + + NOUN bam-4823 19 50 60 60 NUM bam-4823 19 51 in in ADP bam-4823 19 52 1or 1or ADJ bam-4823 19 53 2 2 NUM bam-4823 19 54 - - PUNCT bam-4823 19 55 degree degree NOUN bam-4823 19 56 intervals interval NOUN bam-4823 19 57 ) ) PUNCT bam-4823 19 58 . . PUNCT bam-4823 20 1 consequently consequently ADV bam-4823 20 2 , , PUNCT bam-4823 20 3 few few ADJ bam-4823 20 4 cryo cryo NOUN bam-4823 20 5 - - PUNCT bam-4823 20 6 et et NOUN bam-4823 20 7 studies study NOUN bam-4823 20 8 have have AUX bam-4823 20 9 been be AUX bam-4823 20 10 published publish VERB bam-4823 20 11 using use VERB bam-4823 20 12 vitreous vitreous ADJ bam-4823 20 13 cryo cryo NOUN bam-4823 20 14 - - PUNCT bam-4823 20 15 sections section NOUN bam-4823 20 16 . . PUNCT bam-4823 21 1 recently recently ADV bam-4823 21 2 , , PUNCT bam-4823 21 3 we we PRON bam-4823 21 4 proposed propose VERB bam-4823 21 5 and and CCONJ bam-4823 21 6 have have AUX bam-4823 21 7 developed develop VERB bam-4823 21 8 a a DET bam-4823 21 9 new new ADJ bam-4823 21 10 method method NOUN bam-4823 21 11 for for ADP bam-4823 21 12 preparing prepare VERB bam-4823 21 13 thin thin ADJ bam-4823 21 14 sections section NOUN bam-4823 21 15 of of ADP bam-4823 21 16 vitreously vitreously NOUN bam-4823 21 17 frozen frozen ADJ bam-4823 21 18 cells cell NOUN bam-4823 21 19 and and CCONJ bam-4823 21 20 also also ADV bam-4823 21 21 tissue tissue NOUN bam-4823 21 22 which which PRON bam-4823 21 23 employs employ VERB bam-4823 21 24 focused focused ADJ bam-4823 21 25 - - PUNCT bam-4823 21 26 ionbeam ionbeam NOUN bam-4823 21 27 ( ( PUNCT bam-4823 21 28 fib fib NOUN bam-4823 21 29 ) ) PUNCT bam-4823 21 30 milling milling NOUN bam-4823 21 31 . . PUNCT bam-4823 22 1 13 13 NUM bam-4823 22 2 - - SYM bam-4823 22 3 16 16 NUM bam-4823 22 4 here here ADV bam-4823 22 5 we we PRON bam-4823 22 6 give give VERB bam-4823 22 7 an an DET bam-4823 22 8 overview overview NOUN bam-4823 22 9 of of ADP bam-4823 22 10 the the DET bam-4823 22 11 potential potential NOUN bam-4823 22 12 for for SCONJ bam-4823 22 13 cryo cryo NOUN bam-4823 22 14 - - PUNCT bam-4823 22 15 et et NOUN bam-4823 22 16 applied apply VERB bam-4823 22 17 to to ADP bam-4823 22 18 fib fib ADJ bam-4823 22 19 - - PUNCT bam-4823 22 20 milled mill VERB bam-4823 22 21 muscle muscle NOUN bam-4823 22 22 , , PUNCT bam-4823 22 23 to to PART bam-4823 22 24 resolve resolve VERB bam-4823 22 25 structural structural ADJ bam-4823 22 26 details detail NOUN bam-4823 22 27 of of ADP bam-4823 22 28 the the DET bam-4823 22 29 triad triad ADJ bam-4823 22 30 junction junction NOUN bam-4823 22 31 . . PUNCT bam-4823 23 1 although although SCONJ bam-4823 23 2 much much ADJ bam-4823 23 3 progress progress NOUN bam-4823 23 4 remains remain VERB bam-4823 23 5 to to PART bam-4823 23 6 be be AUX bam-4823 23 7 made make VERB bam-4823 23 8 , , PUNCT bam-4823 23 9 conceivably conceivably ADV bam-4823 23 10 the the DET bam-4823 23 11 macromolecular macromolecular ADJ bam-4823 23 12 organization organization NOUN bam-4823 23 13 of of ADP bam-4823 23 14 the the DET bam-4823 23 15 triad triad NOUN bam-4823 23 16 at at ADP bam-4823 23 17 the the DET bam-4823 23 18 nanometer nanometer NOUN bam-4823 23 19 level level NOUN bam-4823 23 20 of of ADP bam-4823 23 21 detail detail NOUN bam-4823 23 22 will will AUX bam-4823 23 23 be be AUX bam-4823 23 24 achievable achievable ADJ bam-4823 23 25 in in ADP bam-4823 23 26 the the DET bam-4823 23 27 future future NOUN bam-4823 23 28 . . PUNCT bam-4823 24 1 overview overview NOUN bam-4823 24 2 of of ADP bam-4823 24 3 the the DET bam-4823 24 4 fib fib ADJ bam-4823 24 5 milling milling NOUN bam-4823 24 6 technique technique NOUN bam-4823 24 7 thinning thinning NOUN bam-4823 24 8 of of ADP bam-4823 24 9 biological biological ADJ bam-4823 24 10 specimens specimen NOUN bam-4823 24 11 by by ADP bam-4823 24 12 fib fib ADJ bam-4823 24 13 milling milling NOUN bam-4823 24 14 involves involve VERB bam-4823 24 15 irradiation irradiation NOUN bam-4823 24 16 under under ADP bam-4823 24 17 vacuum vacuum NOUN bam-4823 24 18 with with ADP bam-4823 24 19 a a DET bam-4823 24 20 focused focused ADJ bam-4823 24 21 beam beam NOUN bam-4823 24 22 of of ADP bam-4823 24 23 ( ( PUNCT bam-4823 24 24 usually usually ADV bam-4823 24 25 gallium gallium NOUN bam-4823 24 26 ) ) PUNCT bam-4823 24 27 ions ion NOUN bam-4823 24 28 , , PUNCT bam-4823 24 29 which which PRON bam-4823 24 30 scans scan VERB bam-4823 24 31 the the DET bam-4823 24 32 specimen speciman NOUN bam-4823 24 33 in in ADP bam-4823 24 34 a a DET bam-4823 24 35 raster raster NOUN bam-4823 24 36 fashion fashion NOUN bam-4823 24 37 , , PUNCT bam-4823 24 38 removing remove VERB bam-4823 24 39 mass mass NOUN bam-4823 24 40 from from ADP bam-4823 24 41 the the DET bam-4823 24 42 surface surface NOUN bam-4823 24 43 by by ADP bam-4823 24 44 a a DET bam-4823 24 45 sputtering sputtering ADJ bam-4823 24 46 mechanism mechanism NOUN bam-4823 24 47 . . PUNCT bam-4823 25 1 fib fib ADJ bam-4823 25 2 instruments instrument NOUN bam-4823 25 3 are be AUX bam-4823 25 4 often often ADV bam-4823 25 5 combined combine VERB bam-4823 25 6 with with ADP bam-4823 25 7 a a DET bam-4823 25 8 scanning scanning NOUN bam-4823 25 9 electron electron NOUN bam-4823 25 10 microscope microscope NOUN bam-4823 25 11 ( ( PUNCT bam-4823 25 12 sem sem NOUN bam-4823 25 13 ) ) PUNCT bam-4823 25 14 so so SCONJ bam-4823 25 15 that that SCONJ bam-4823 25 16 the the DET bam-4823 25 17 location location NOUN bam-4823 25 18 and and CCONJ bam-4823 25 19 progress progress NOUN bam-4823 25 20 of of ADP bam-4823 25 21 the the DET bam-4823 25 22 milling milling NOUN bam-4823 25 23 can can AUX bam-4823 25 24 be be AUX bam-4823 25 25 monitored monitor VERB bam-4823 25 26 . . PUNCT bam-4823 26 1 currently currently ADV bam-4823 26 2 , , PUNCT bam-4823 26 3 fib fib ADJ bam-4823 26 4 - - PUNCT bam-4823 26 5 sem sem NOUN bam-4823 26 6 tomography tomography NOUN bam-4823 26 7 is be AUX bam-4823 26 8 a a DET bam-4823 26 9 popular popular ADJ bam-4823 26 10 technique technique NOUN bam-4823 26 11 , , PUNCT bam-4823 26 12 17 17 NUM bam-4823 26 13 often often ADV bam-4823 26 14 applied apply VERB bam-4823 26 15 to to ADP bam-4823 26 16 a a DET bam-4823 26 17 block block NOUN bam-4823 26 18 of of ADP bam-4823 26 19 resin resin NOUN bam-4823 26 20 - - PUNCT bam-4823 26 21 embedded embed VERB bam-4823 26 22 , , PUNCT bam-4823 26 23 stained stain VERB bam-4823 26 24 biological biological ADJ bam-4823 26 25 specimen speciman NOUN bam-4823 26 26 . . PUNCT bam-4823 27 1 the the DET bam-4823 27 2 block block NOUN bam-4823 27 3 face face NOUN bam-4823 27 4 is be AUX bam-4823 27 5 imaged image VERB bam-4823 27 6 by by ADP bam-4823 27 7 sem sem PROPN bam-4823 27 8 after after ADP bam-4823 27 9 fib fib ADJ bam-4823 27 10 - - PUNCT bam-4823 27 11 milling milling NOUN bam-4823 27 12 to to PART bam-4823 27 13 remove remove VERB bam-4823 27 14 thin thin ADJ bam-4823 27 15 layers layer NOUN bam-4823 27 16 of of ADP bam-4823 27 17 the the DET bam-4823 27 18 specimen speciman NOUN bam-4823 27 19 , , PUNCT bam-4823 27 20 and and CCONJ bam-4823 27 21 then then ADV bam-4823 27 22 tomograms tomogram NOUN bam-4823 27 23 are be AUX bam-4823 27 24 formed form VERB bam-4823 27 25 from from ADP bam-4823 27 26 the the DET bam-4823 27 27 stack stack NOUN bam-4823 27 28 of of ADP bam-4823 27 29 images image NOUN bam-4823 27 30 obtained obtain VERB bam-4823 27 31 after after SCONJ bam-4823 27 32 each each DET bam-4823 27 33 successive successive ADJ bam-4823 27 34 layer layer NOUN bam-4823 27 35 of of ADP bam-4823 27 36 the the DET bam-4823 27 37 specimen speciman NOUN bam-4823 27 38 is be AUX bam-4823 27 39 removed remove VERB bam-4823 27 40 . . PUNCT bam-4823 28 1 fib fib ADJ bam-4823 28 2 - - PUNCT bam-4823 28 3 sem sem NOUN bam-4823 28 4 tomography tomography NOUN bam-4823 28 5 is be AUX bam-4823 28 6 useful useful ADJ bam-4823 28 7 for for ADP bam-4823 28 8 visualizing visualize VERB bam-4823 28 9 rather rather ADV bam-4823 28 10 large large ADJ bam-4823 28 11 volumes volume NOUN bam-4823 28 12 of of ADP bam-4823 28 13 specimens specimen NOUN bam-4823 28 14 at at ADP bam-4823 28 15 modest modest ADJ bam-4823 28 16 resolution resolution NOUN bam-4823 28 17 but but CCONJ bam-4823 28 18 is be AUX bam-4823 28 19 not not PART bam-4823 28 20 generally generally ADV bam-4823 28 21 useful useful ADJ bam-4823 28 22 for for ADP bam-4823 28 23 visualizing visualize VERB bam-4823 28 24 macromolecular macromolecular ADJ bam-4823 28 25 assemblies assembly NOUN bam-4823 28 26 and and CCONJ bam-4823 28 27 is be AUX bam-4823 28 28 therefore therefore ADV bam-4823 28 29 not not PART bam-4823 28 30 treated treat VERB bam-4823 28 31 further far ADV bam-4823 28 32 here here ADV bam-4823 28 33 . . PUNCT bam-4823 29 1 rather rather ADV bam-4823 29 2 , , PUNCT bam-4823 29 3 we we PRON bam-4823 29 4 focus focus VERB bam-4823 29 5 on on ADP bam-4823 29 6 the the DET bam-4823 29 7 application application NOUN bam-4823 29 8 of of ADP bam-4823 29 9 fib fib NOUN bam-4823 29 10 milling milling NOUN bam-4823 29 11 to to PART bam-4823 29 12 create create VERB bam-4823 29 13 thin thin ADJ bam-4823 29 14 sections section NOUN bam-4823 29 15 of of ADP bam-4823 29 16 vitreously vitreously NOUN bam-4823 29 17 frozen frozen ADJ bam-4823 29 18 specimens specimen NOUN bam-4823 29 19 for for ADP bam-4823 29 20 subsequent subsequent ADJ bam-4823 29 21 transfer transfer NOUN bam-4823 29 22 to to ADP bam-4823 29 23 a a DET bam-4823 29 24 tem tem NOUN bam-4823 29 25 for for ADP bam-4823 29 26 cryo cryo NOUN bam-4823 29 27 - - PUNCT bam-4823 29 28 et et NOUN bam-4823 29 29 . . PUNCT bam-4823 30 1 the the DET bam-4823 30 2 utility utility NOUN bam-4823 30 3 of of ADP bam-4823 30 4 fib fib ADJ bam-4823 30 5 milling milling NOUN bam-4823 30 6 for for ADP bam-4823 30 7 preparing prepare VERB bam-4823 30 8 thin thin ADJ bam-4823 30 9 sections section NOUN bam-4823 30 10 of of ADP bam-4823 30 11 vitreously vitreously NOUN bam-4823 30 12 frozen frozen ADJ bam-4823 30 13 cells cell NOUN bam-4823 30 14 for for ADP bam-4823 30 15 cryo cryo NOUN bam-4823 30 16 - - PUNCT bam-4823 30 17 et et NOUN bam-4823 30 18 was be AUX bam-4823 30 19 first first ADV bam-4823 30 20 demonstrated demonstrate VERB bam-4823 30 21 by by ADP bam-4823 30 22 marko marko PROPN bam-4823 30 23 and and CCONJ bam-4823 30 24 colleagues colleague NOUN bam-4823 30 25 . . PUNCT bam-4823 31 1 14 14 NUM bam-4823 31 2 - - SYM bam-4823 31 3 16 16 NUM bam-4823 31 4 remarkably remarkably ADV bam-4823 31 5 , , PUNCT bam-4823 31 6 when when SCONJ bam-4823 31 7 suitable suitable ADJ bam-4823 31 8 precautions precaution NOUN bam-4823 31 9 are be AUX bam-4823 31 10 taken take VERB bam-4823 31 11 , , PUNCT bam-4823 31 12 fibprepared fibprepared ADJ bam-4823 31 13 cryo cryo NOUN bam-4823 31 14 - - PUNCT bam-4823 31 15 sections section NOUN bam-4823 31 16 do do AUX bam-4823 31 17 not not PART bam-4823 31 18 suffer suffer VERB bam-4823 31 19 from from ADP bam-4823 31 20 the the DET bam-4823 31 21 artefacts artefact NOUN bam-4823 31 22 mentioned mention VERB bam-4823 31 23 above above ADP bam-4823 31 24 that that DET bam-4823 31 25 plague plague NOUN bam-4823 31 26 cryo cryo NOUN bam-4823 31 27 - - PUNCT bam-4823 31 28 ultramicrotomy ultramicrotomy NOUN bam-4823 31 29 , , PUNCT bam-4823 31 30 and and CCONJ bam-4823 31 31 fib fib ADJ bam-4823 31 32 - - PUNCT bam-4823 31 33 specific specific ADJ bam-4823 31 34 damage damage NOUN bam-4823 31 35 is be AUX bam-4823 31 36 minimal minimal ADJ bam-4823 31 37 : : PUNCT bam-4823 31 38 damage damage NOUN bam-4823 31 39 and and CCONJ bam-4823 31 40 implantation implantation NOUN bam-4823 31 41 from from ADP bam-4823 31 42 ga ga PROPN bam-4823 31 43 + + SYM bam-4823 31 44 ions ion NOUN bam-4823 31 45 , , PUNCT bam-4823 31 46 or or CCONJ bam-4823 31 47 devitrification devitrification NOUN bam-4823 31 48 due due ADJ bam-4823 31 49 to to ADP bam-4823 31 50 warming warming NOUN bam-4823 31 51 , , PUNCT bam-4823 31 52 are be AUX bam-4823 31 53 restricted restrict VERB bam-4823 31 54 a a DET bam-4823 31 55 depth depth NOUN bam-4823 31 56 of of ADP bam-4823 31 57 about about ADV bam-4823 31 58 10 10 NUM bam-4823 31 59 nm nm NOUN bam-4823 31 60 . . PROPN bam-4823 31 61 15 15 NUM bam-4823 31 62 , , PUNCT bam-4823 31 63 18 18 NUM bam-4823 31 64 these these PRON bam-4823 31 65 demonstrated demonstrate VERB bam-4823 31 66 advantages advantage NOUN bam-4823 31 67 of of ADP bam-4823 31 68 fib fib NOUN bam-4823 31 69 - - PUNCT bam-4823 31 70 milling milling NOUN bam-4823 31 71 over over ADP bam-4823 31 72 cryo cryo NOUN bam-4823 31 73 - - PUNCT bam-4823 31 74 ultramicrotomy ultramicrotomy NOUN bam-4823 31 75 for for ADP bam-4823 31 76 creating create VERB bam-4823 31 77 thin thin ADJ bam-4823 31 78 sections section NOUN bam-4823 31 79 of of ADP bam-4823 31 80 vitreously vitreously NOUN bam-4823 31 81 frozen frozen ADJ bam-4823 31 82 biological biological ADJ bam-4823 31 83 specimens specimen NOUN bam-4823 31 84 for for ADP bam-4823 31 85 cryo cryo NOUN bam-4823 31 86 - - PUNCT bam-4823 31 87 et et NOUN bam-4823 31 88 have have AUX bam-4823 31 89 stimulated stimulate VERB bam-4823 31 90 several several ADJ bam-4823 31 91 research research NOUN bam-4823 31 92 groups group NOUN bam-4823 31 93 to to PART bam-4823 31 94 develop develop VERB bam-4823 31 95 and and CCONJ bam-4823 31 96 implement implement VERB bam-4823 31 97 the the DET bam-4823 31 98 methodology methodology NOUN bam-4823 31 99 . . PUNCT bam-4823 32 1 our our PRON bam-4823 32 2 laboratory laboratory NOUN bam-4823 32 3 ’s ’s PART bam-4823 32 4 experience experience NOUN bam-4823 32 5 , , PUNCT bam-4823 32 6 which which PRON bam-4823 32 7 is be AUX bam-4823 32 8 not not PART bam-4823 32 9 unique unique ADJ bam-4823 32 10 , , PUNCT bam-4823 32 11 indicates indicate VERB bam-4823 32 12 that that SCONJ bam-4823 32 13 the the DET bam-4823 32 14 field field NOUN bam-4823 32 15 is be AUX bam-4823 32 16 still still ADV bam-4823 32 17 in in ADP bam-4823 32 18 its its PRON bam-4823 32 19 early early ADJ bam-4823 32 20 stages stage NOUN bam-4823 32 21 and and CCONJ bam-4823 32 22 , , PUNCT bam-4823 32 23 at at ADP bam-4823 32 24 present present ADJ bam-4823 32 25 , , PUNCT bam-4823 32 26 the the DET bam-4823 32 27 techniques technique NOUN bam-4823 32 28 that that PRON bam-4823 32 29 have have AUX bam-4823 32 30 been be AUX bam-4823 32 31 described describe VERB bam-4823 32 32 remain remain VERB bam-4823 32 33 cumbersome cumbersome ADJ bam-4823 32 34 , , PUNCT bam-4823 32 35 far far ADV bam-4823 32 36 from from ADP bam-4823 32 37 routine routine ADJ bam-4823 32 38 , , PUNCT bam-4823 32 39 and and CCONJ bam-4823 32 40 require require VERB bam-4823 32 41 custom custom ADV bam-4823 32 42 - - PUNCT bam-4823 32 43 modified modify VERB bam-4823 32 44 instruments instrument NOUN bam-4823 32 45 and and CCONJ bam-4823 32 46 tools tool NOUN bam-4823 32 47 . . PUNCT bam-4823 33 1 to to ADP bam-4823 33 2 date date NOUN bam-4823 33 3 , , PUNCT bam-4823 33 4 most most ADJ bam-4823 33 5 success success NOUN bam-4823 33 6 has have AUX bam-4823 33 7 been be AUX bam-4823 33 8 achieved achieve VERB bam-4823 33 9 with with ADP bam-4823 33 10 strategies strategy NOUN bam-4823 33 11 that that PRON bam-4823 33 12 involved involve VERB bam-4823 33 13 depositing deposit VERB bam-4823 33 14 or or CCONJ bam-4823 33 15 culturing culture VERB bam-4823 33 16 cells cell NOUN bam-4823 33 17 on on ADP bam-4823 33 18 tem tem PROPN bam-4823 33 19 grids grid NOUN bam-4823 33 20 , , PUNCT bam-4823 33 21 and and CCONJ bam-4823 33 22 freezing freeze VERB bam-4823 33 23 them they PRON bam-4823 33 24 by by ADP bam-4823 33 25 plunging plunge VERB bam-4823 33 26 the the DET bam-4823 33 27 grids grid NOUN bam-4823 33 28 into into ADP bam-4823 33 29 a a DET bam-4823 33 30 cryogen cryogen NOUN bam-4823 33 31 ( ( PUNCT bam-4823 33 32 “ " PUNCT bam-4823 33 33 plunge plunge NOUN bam-4823 33 34 freezing freezing NOUN bam-4823 33 35 ” " PUNCT bam-4823 33 36 ) ) PUNCT bam-4823 33 37 . . PUNCT bam-4823 34 1 then then ADV bam-4823 34 2 the the DET bam-4823 34 3 grids grid NOUN bam-4823 34 4 are be AUX bam-4823 34 5 transferred transfer VERB bam-4823 34 6 into into ADP bam-4823 34 7 the the DET bam-4823 34 8 fib fib ADJ bam-4823 34 9 - - PUNCT bam-4823 34 10 sem sem NOUN bam-4823 34 11 instrument instrument NOUN bam-4823 34 12 for for ADP bam-4823 34 13 thinning thin VERB bam-4823 34 14 a a DET bam-4823 34 15 few few ADJ bam-4823 34 16 limited limited ADJ bam-4823 34 17 regions region NOUN bam-4823 34 18 ( ( PUNCT bam-4823 34 19 typically typically ADV bam-4823 34 20 hundreds hundred NOUN bam-4823 34 21 of of ADP bam-4823 34 22 µm µm ADP bam-4823 34 23 2 2 NUM bam-4823 34 24 in in ADP bam-4823 34 25 area area NOUN bam-4823 34 26 ) ) PUNCT bam-4823 34 27 . . PUNCT bam-4823 35 1 finally finally ADV bam-4823 35 2 , , PUNCT bam-4823 35 3 the the DET bam-4823 35 4 grids grid NOUN bam-4823 35 5 are be AUX bam-4823 35 6 transferred transfer VERB bam-4823 35 7 into into ADP bam-4823 35 8 the the DET bam-4823 35 9 cryotem cryotem NOUN bam-4823 35 10 . . PUNCT bam-4823 36 1 all all DET bam-4823 36 2 these these DET bam-4823 36 3 steps step NOUN bam-4823 36 4 must must AUX bam-4823 36 5 be be AUX bam-4823 36 6 done do VERB bam-4823 36 7 while while SCONJ bam-4823 36 8 maintaining maintain VERB bam-4823 36 9 the the DET bam-4823 36 10 temperature temperature NOUN bam-4823 36 11 below below ADP bam-4823 36 12 the the DET bam-4823 36 13 devitrification devitrification NOUN bam-4823 36 14 temperature temperature NOUN bam-4823 36 15 ( ( PUNCT bam-4823 36 16 < < X bam-4823 36 17 -140 -140 PROPN bam-4823 36 18 ° ° PROPN bam-4823 36 19 c c NOUN bam-4823 36 20 ) ) PUNCT bam-4823 36 21 and and CCONJ bam-4823 36 22 minimizing minimize VERB bam-4823 36 23 frost frost NOUN bam-4823 36 24 or or CCONJ bam-4823 36 25 warming warming NOUN bam-4823 36 26 during during ADP bam-4823 36 27 cryo cryo NOUN bam-4823 36 28 - - PUNCT bam-4823 36 29 transfer transfer NOUN bam-4823 36 30 steps step NOUN bam-4823 36 31 . . PUNCT bam-4823 37 1 several several ADJ bam-4823 37 2 groups group NOUN bam-4823 37 3 have have AUX bam-4823 37 4 implemented implement VERB bam-4823 37 5 this this DET bam-4823 37 6 strategy strategy NOUN bam-4823 37 7 15,19 15,19 NUM bam-4823 37 8 - - SYM bam-4823 37 9 23 23 NUM bam-4823 37 10 and and CCONJ bam-4823 37 11 have have AUX bam-4823 37 12 applied apply VERB bam-4823 37 13 it it PRON bam-4823 37 14 to to ADP bam-4823 37 15 bacteria bacteria NOUN bam-4823 37 16 and and CCONJ bam-4823 37 17 unicellular unicellular ADJ bam-4823 37 18 eukaryotes eukaryote NOUN bam-4823 37 19 . . PUNCT bam-4823 38 1 the the DET bam-4823 38 2 cryo cryo NOUN bam-4823 38 3 - - PUNCT bam-4823 38 4 et et NOUN bam-4823 38 5 analysis analysis NOUN bam-4823 38 6 of of ADP bam-4823 38 7 dictyostelium dictyostelium NOUN bam-4823 38 8 discoideum discoideum NOUN bam-4823 38 9 cells cell NOUN bam-4823 38 10 described describe VERB bam-4823 38 11 by by ADP bam-4823 38 12 rigort rigort PROPN bam-4823 38 13 et et PROPN bam-4823 38 14 al al PROPN bam-4823 38 15 . . PROPN bam-4823 38 16 21 21 NUM bam-4823 38 17 is be AUX bam-4823 38 18 particularly particularly ADV bam-4823 38 19 noteworthy noteworthy ADJ bam-4823 38 20 . . PUNCT bam-4823 39 1 the the DET bam-4823 39 2 fib fib ADJ bam-4823 39 3 - - PUNCT bam-4823 39 4 milled mill VERB bam-4823 39 5 region region NOUN bam-4823 39 6 was be AUX bam-4823 39 7 formed form VERB bam-4823 39 8 by by ADP bam-4823 39 9 ablating ablate VERB bam-4823 39 10 regions region NOUN bam-4823 39 11 from from ADP bam-4823 39 12 the the DET bam-4823 39 13 upper upper ADJ bam-4823 39 14 and and CCONJ bam-4823 39 15 lower low ADJ bam-4823 39 16 portions portion NOUN bam-4823 39 17 of of ADP bam-4823 39 18 the the DET bam-4823 39 19 cell cell NOUN bam-4823 39 20 to to PART bam-4823 39 21 create create VERB bam-4823 39 22 a a DET bam-4823 39 23 300 300 NUM bam-4823 39 24 - - PUNCT bam-4823 39 25 nm nm NOUN bam-4823 39 26 - - ADJ bam-4823 39 27 thick thick ADJ bam-4823 39 28 lamella lamella NOUN bam-4823 39 29 in in ADP bam-4823 39 30 the the DET bam-4823 39 31 middle middle NOUN bam-4823 39 32 of of ADP bam-4823 39 33 the the DET bam-4823 39 34 cell cell NOUN bam-4823 39 35 that that PRON bam-4823 39 36 was be AUX bam-4823 39 37 supported support VERB bam-4823 39 38 on on ADP bam-4823 39 39 the the DET bam-4823 39 40 sides side NOUN bam-4823 39 41 by by ADP bam-4823 39 42 non non ADJ bam-4823 39 43 - - ADJ bam-4823 39 44 milled milled ADJ bam-4823 39 45 regions region NOUN bam-4823 39 46 ; ; PUNCT bam-4823 39 47 this this PRON bam-4823 39 48 differs differ VERB bam-4823 39 49 from from ADP bam-4823 39 50 the the DET bam-4823 39 51 more more ADV bam-4823 39 52 straightforward straightforward ADJ bam-4823 39 53 procedure procedure NOUN bam-4823 39 54 followed follow VERB bam-4823 39 55 for for ADP bam-4823 39 56 smaller small ADJ bam-4823 39 57 cells cell NOUN bam-4823 39 58 in in ADP bam-4823 39 59 which which PRON bam-4823 39 60 the the DET bam-4823 39 61 cell cell NOUN bam-4823 39 62 is be AUX bam-4823 39 63 milled mill VERB bam-4823 39 64 from from ADP bam-4823 39 65 just just ADV bam-4823 39 66 one one NUM bam-4823 39 67 side side NOUN bam-4823 39 68 , , PUNCT bam-4823 39 69 the the DET bam-4823 39 70 side side NOUN bam-4823 39 71 facing face VERB bam-4823 39 72 away away ADV bam-4823 39 73 from from ADP bam-4823 39 74 the the DET bam-4823 39 75 grid grid NOUN bam-4823 39 76 surface surface NOUN bam-4823 39 77 . . PUNCT bam-4823 40 1 a a DET bam-4823 40 2 similar similar ADJ bam-4823 40 3 twosided twoside VERB bam-4823 40 4 milling milling NOUN bam-4823 40 5 strategy strategy NOUN bam-4823 40 6 is be AUX bam-4823 40 7 also also ADV bam-4823 40 8 necessary necessary ADJ bam-4823 40 9 for for ADP bam-4823 40 10 tissue tissue NOUN bam-4823 40 11 , , PUNCT bam-4823 40 12 as as SCONJ bam-4823 40 13 we we PRON bam-4823 40 14 discuss discuss VERB bam-4823 40 15 below below ADV bam-4823 40 16 . . PUNCT bam-4823 41 1 another another DET bam-4823 41 2 notable notable ADJ bam-4823 41 3 achievement achievement NOUN bam-4823 41 4 of of ADP bam-4823 41 5 this this DET bam-4823 41 6 study study NOUN bam-4823 41 7 is be AUX bam-4823 41 8 that that SCONJ bam-4823 41 9 ten ten NUM bam-4823 41 10 nuclear nuclear ADJ bam-4823 41 11 pore pore ADJ bam-4823 41 12 complexes complex NOUN bam-4823 41 13 ( ( PUNCT bam-4823 41 14 which which PRON bam-4823 41 15 span span VERB bam-4823 41 16 two two NUM bam-4823 41 17 membranes membrane NOUN bam-4823 41 18 , , PUNCT bam-4823 41 19 as as SCONJ bam-4823 41 20 do do VERB bam-4823 41 21 triad triad VERB bam-4823 41 22 junctions junction NOUN bam-4823 41 23 ) ) PUNCT bam-4823 41 24 were be AUX bam-4823 41 25 identified identify VERB bam-4823 41 26 visually visually ADV bam-4823 41 27 in in ADP bam-4823 41 28 one one NUM bam-4823 41 29 tomogram tomogram NOUN bam-4823 41 30 , , PUNCT bam-4823 41 31 and and CCONJ bam-4823 41 32 the the DET bam-4823 41 33 authors author NOUN bam-4823 41 34 successfully successfully ADV bam-4823 41 35 applied apply VERB bam-4823 41 36 sub sub NOUN bam-4823 41 37 - - NOUN bam-4823 41 38 tomogram tomogram NOUN bam-4823 41 39 averaging averaging NOUN bam-4823 41 40 to to PART bam-4823 41 41 obtain obtain VERB bam-4823 41 42 a a DET bam-4823 41 43 reconstruction reconstruction NOUN bam-4823 41 44 of of ADP bam-4823 41 45 the the DET bam-4823 41 46 nuclear nuclear ADJ bam-4823 41 47 pore pore NOUN bam-4823 41 48 having have VERB bam-4823 41 49 a a DET bam-4823 41 50 resolution resolution NOUN bam-4823 41 51 of of ADP bam-4823 41 52 7.9 7.9 NUM bam-4823 41 53 nm nm NOUN bam-4823 41 54 . . PUNCT bam-4823 42 1 fib fib ADJ bam-4823 42 2 milling milling NOUN bam-4823 42 3 of of ADP bam-4823 42 4 tissue tissue NOUN bam-4823 42 5 for for ADP bam-4823 42 6 ctem ctem NOUN bam-4823 42 7 ( ( PUNCT bam-4823 42 8 albany albany PROPN bam-4823 42 9 implementation implementation PROPN bam-4823 42 10 ) ) PUNCT bam-4823 42 11 13 13 NUM bam-4823 42 12 specimen speciman NOUN bam-4823 42 13 preparation preparation NOUN bam-4823 42 14 techniques technique NOUN bam-4823 42 15 that that PRON bam-4823 42 16 involve involve VERB bam-4823 42 17 deposition deposition NOUN bam-4823 42 18 directly directly ADV bam-4823 42 19 onto onto ADP bam-4823 42 20 em em PRON bam-4823 42 21 grids grid NOUN bam-4823 42 22 are be AUX bam-4823 42 23 not not PART bam-4823 42 24 easily easily ADV bam-4823 42 25 adapted adapt VERB bam-4823 42 26 to to ADP bam-4823 42 27 bulk bulk ADJ bam-4823 42 28 tissue tissue NOUN bam-4823 42 29 specimens specimen NOUN bam-4823 42 30 , , PUNCT bam-4823 42 31 which which PRON bam-4823 42 32 are be AUX bam-4823 42 33 too too ADV bam-4823 42 34 large large ADJ bam-4823 42 35 for for ADP bam-4823 42 36 plunge plunge NOUN bam-4823 42 37 - - PUNCT bam-4823 42 38 freezing freezing NOUN bam-4823 42 39 , , PUNCT bam-4823 42 40 and and CCONJ bam-4823 42 41 therefore therefore ADV bam-4823 42 42 must must AUX bam-4823 42 43 be be AUX bam-4823 42 44 highpressure highpressure ADJ bam-4823 42 45 frozen frozen ADJ bam-4823 42 46 ( ( PUNCT bam-4823 42 47 hpf hpf NOUN bam-4823 42 48 ) ) PUNCT bam-4823 42 49 and and CCONJ bam-4823 42 50 then then ADV bam-4823 42 51 “ " PUNCT bam-4823 42 52 pre pre ADJ bam-4823 42 53 - - ADJ bam-4823 42 54 trimmed trimmed ADJ bam-4823 42 55 ” " PUNCT bam-4823 42 56 in in ADP bam-4823 42 57 a a DET bam-4823 42 58 cryo cryo NOUN bam-4823 42 59 - - PUNCT bam-4823 42 60 ultramicrotome ultramicrotome NOUN bam-4823 42 61 before before SCONJ bam-4823 42 62 cryo cryo NOUN bam-4823 42 63 - - PUNCT bam-4823 42 64 fib fib NOUN bam-4823 42 65 milling milling NOUN bam-4823 42 66 is be AUX bam-4823 42 67 used use VERB bam-4823 42 68 to to PART bam-4823 42 69 create create VERB bam-4823 42 70 the the DET bam-4823 42 71 final final ADJ bam-4823 42 72 thinned thin VERB bam-4823 42 73 lamellae lamellae NOUN bam-4823 42 74 . . PUNCT bam-4823 43 1 the the DET bam-4823 43 2 methodology methodology NOUN bam-4823 43 3 that that PRON bam-4823 43 4 we we PRON bam-4823 43 5 have have AUX bam-4823 43 6 developed develop VERB bam-4823 43 7 allows allow VERB bam-4823 43 8 specimens specimen NOUN bam-4823 43 9 to to PART bam-4823 43 10 be be AUX bam-4823 43 11 frozen freeze VERB bam-4823 43 12 in in ADP bam-4823 43 13 commercially commercially ADV bam-4823 43 14 available available ADJ bam-4823 43 15 hpf hpf NOUN bam-4823 43 16 specimen speciman NOUN bam-4823 43 17 carriers carrier NOUN bam-4823 43 18 . . PUNCT bam-4823 44 1 13 13 NUM bam-4823 44 2 . . PUNCT bam-4823 45 1 customized customize VERB bam-4823 45 2 accessories accessory NOUN bam-4823 45 3 have have AUX bam-4823 45 4 been be AUX bam-4823 45 5 fabricated fabricate VERB bam-4823 45 6 for for ADP bam-4823 45 7 holding hold VERB bam-4823 45 8 , , PUNCT bam-4823 45 9 manipulating manipulate VERB bam-4823 45 10 , , PUNCT bam-4823 45 11 and and CCONJ bam-4823 45 12 storing store VERB bam-4823 45 13 the the DET bam-4823 45 14 specimen speciman NOUN bam-4823 45 15 through through ADP bam-4823 45 16 all all PRON bam-4823 45 17 of of ADP bam-4823 45 18 the the DET bam-4823 45 19 steps step NOUN bam-4823 45 20 of of ADP bam-4823 45 21 the the DET bam-4823 45 22 procedure procedure NOUN bam-4823 45 23 . . PUNCT bam-4823 46 1 an an DET bam-4823 46 2 outline outline NOUN bam-4823 46 3 of of ADP bam-4823 46 4 skeletal skeletal ADJ bam-4823 46 5 muscle muscle NOUN bam-4823 46 6 triad triad VERB bam-4823 46 7 by by ADP bam-4823 46 8 fib fib NOUN bam-4823 46 9 milling milling NOUN bam-4823 46 10 and and CCONJ bam-4823 46 11 cryo cryo NOUN bam-4823 46 12 - - PUNCT bam-4823 46 13 et et PROPN bam-4823 46 14 eur eur PROPN bam-4823 46 15 j j PROPN bam-4823 46 16 transl transl PROPN bam-4823 47 1 myol myol PROPN bam-4823 47 2 basic basic ADJ bam-4823 47 3 appl appl NOUN bam-4823 47 4 myol myol NOUN bam-4823 47 5 2015 2015 NUM bam-4823 47 6 ; ; PUNCT bam-4823 47 7 25 25 NUM bam-4823 47 8 ( ( PUNCT bam-4823 47 9 1 1 NUM bam-4823 47 10 ): ): PUNCT bam-4823 47 11 49 49 NUM bam-4823 47 12 - - SYM bam-4823 47 13 56 56 NUM bam-4823 47 14 51 51 NUM bam-4823 47 15 the the DET bam-4823 47 16 main main ADJ bam-4823 47 17 steps step NOUN bam-4823 47 18 of of ADP bam-4823 47 19 the the DET bam-4823 47 20 procedure procedure NOUN bam-4823 47 21 ( ( PUNCT bam-4823 47 22 all all PRON bam-4823 47 23 carried carry VERB bam-4823 47 24 out out ADP bam-4823 47 25 below below ADP bam-4823 47 26 140 140 NUM bam-4823 47 27 ° ° NOUN bam-4823 47 28 c c NOUN bam-4823 47 29 ) ) PUNCT bam-4823 47 30 is be AUX bam-4823 47 31 here here ADV bam-4823 47 32 presented present VERB bam-4823 47 33 and and CCONJ bam-4823 47 34 illustrated illustrate VERB bam-4823 47 35 in in ADP bam-4823 47 36 figure figure NOUN bam-4823 47 37 1 1 NUM bam-4823 47 38 . . PUNCT bam-4823 48 1 we we PRON bam-4823 48 2 have have AUX bam-4823 48 3 implemented implement VERB bam-4823 48 4 the the DET bam-4823 48 5 procedures procedure NOUN bam-4823 48 6 using use VERB bam-4823 48 7 a a DET bam-4823 48 8 fib fib ADJ bam-4823 48 9 – – PUNCT bam-4823 48 10 sem sem NOUN bam-4823 48 11 instrument instrument NOUN bam-4823 48 12 from from ADP bam-4823 48 13 carl carl PROPN bam-4823 48 14 zeiss zeiss PROPN bam-4823 48 15 ( ( PUNCT bam-4823 48 16 oberkochen oberkochen PROPN bam-4823 48 17 , , PUNCT bam-4823 48 18 germany germany PROPN bam-4823 48 19 ) ) PUNCT bam-4823 48 20 equipped equip VERB bam-4823 48 21 with with ADP bam-4823 48 22 a a DET bam-4823 48 23 cryo cryo NOUN bam-4823 48 24 - - PUNCT bam-4823 48 25 stage stage NOUN bam-4823 48 26 and and CCONJ bam-4823 48 27 cryo cryo NOUN bam-4823 48 28 - - PUNCT bam-4823 48 29 transfer transfer NOUN bam-4823 48 30 accessories accessory NOUN bam-4823 48 31 that that PRON bam-4823 48 32 are be AUX bam-4823 48 33 available available ADJ bam-4823 48 34 from from ADP bam-4823 48 35 leica leica PROPN bam-4823 48 36 microsystems microsystem NOUN bam-4823 48 37 ( ( PUNCT bam-4823 48 38 vienna vienna PROPN bam-4823 48 39 , , PUNCT bam-4823 48 40 austria austria PROPN bam-4823 48 41 ) ) PUNCT bam-4823 48 42 . . PUNCT bam-4823 49 1 for for ADP bam-4823 49 2 a a DET bam-4823 49 3 more more ADV bam-4823 49 4 detailed detailed ADJ bam-4823 49 5 description description NOUN bam-4823 49 6 of of ADP bam-4823 49 7 method method NOUN bam-4823 49 8 and and CCONJ bam-4823 49 9 hardware hardware NOUN bam-4823 49 10 see see VERB bam-4823 49 11 hsieh hsieh PROPN bam-4823 49 12 et et PROPN bam-4823 49 13 al al PROPN bam-4823 49 14 . . PROPN bam-4823 50 1 ( ( PUNCT bam-4823 50 2 2014 2014 NUM bam-4823 50 3 ) ) PUNCT bam-4823 50 4 . . PUNCT bam-4823 51 1 13 13 NUM bam-4823 51 2 fig fig NOUN bam-4823 51 3 1 1 NUM bam-4823 51 4 . . PUNCT bam-4823 51 5 preparation preparation NOUN bam-4823 51 6 of of ADP bam-4823 51 7 tissue tissue NOUN bam-4823 51 8 for for ADP bam-4823 51 9 cryo cryo NOUN bam-4823 51 10 - - PUNCT bam-4823 51 11 fib fib NOUN bam-4823 51 12 milling milling NOUN bam-4823 51 13 . . PUNCT bam-4823 52 1 a. a. NOUN bam-4823 52 2 high high ADJ bam-4823 52 3 - - PUNCT bam-4823 52 4 pressure pressure NOUN bam-4823 52 5 freezing freezing NOUN bam-4823 52 6 . . PUNCT bam-4823 53 1 bal bal ADJ bam-4823 53 2 - - ADJ bam-4823 53 3 tec tec ADJ bam-4823 53 4 hpm hpm NOUN bam-4823 53 5 010 010 NUM bam-4823 53 6 high high ADJ bam-4823 53 7 - - PUNCT bam-4823 53 8 pressure pressure NOUN bam-4823 53 9 freezer freezer NOUN bam-4823 53 10 with with ADP bam-4823 53 11 close close NOUN bam-4823 53 12 - - PUNCT bam-4823 53 13 up up NOUN bam-4823 53 14 of of ADP bam-4823 53 15 a a DET bam-4823 53 16 3 3 NUM bam-4823 53 17 - - PUNCT bam-4823 53 18 mm mm NOUN bam-4823 53 19 diameter diameter NOUN bam-4823 53 20 slot slot NOUN bam-4823 53 21 - - PUNCT bam-4823 53 22 type type NOUN bam-4823 53 23 specimen speciman NOUN bam-4823 53 24 carrier carrier NOUN bam-4823 53 25 ( ( PUNCT bam-4823 53 26 sc sc PROPN bam-4823 53 27 ) ) PUNCT bam-4823 53 28 ( ( PUNCT bam-4823 53 29 i i NOUN bam-4823 53 30 ) ) PUNCT bam-4823 53 31 and and CCONJ bam-4823 53 32 the the DET bam-4823 53 33 freezing freezing ADJ bam-4823 53 34 head head NOUN bam-4823 53 35 ( ( PUNCT bam-4823 53 36 ii ii NOUN bam-4823 53 37 ) ) PUNCT bam-4823 53 38 . . PUNCT bam-4823 54 1 b. b. PROPN bam-4823 55 1 the the DET bam-4823 55 2 front front ADJ bam-4823 55 3 part part NOUN bam-4823 55 4 of of ADP bam-4823 55 5 an an DET bam-4823 55 6 intermediate intermediate ADJ bam-4823 55 7 specimen speciman NOUN bam-4823 55 8 holder holder NOUN bam-4823 55 9 ( ( PUNCT bam-4823 55 10 ish ish PROPN bam-4823 55 11 ) ) PUNCT bam-4823 55 12 , , PUNCT bam-4823 55 13 shown show VERB bam-4823 55 14 holding hold VERB bam-4823 55 15 a a DET bam-4823 55 16 specimen speciman NOUN bam-4823 55 17 carrier carrier NOUN bam-4823 55 18 ( ( PUNCT bam-4823 55 19 sc sc PROPN bam-4823 55 20 ) ) PUNCT bam-4823 55 21 ; ; PUNCT bam-4823 55 22 arrow arrow NOUN bam-4823 55 23 shows show VERB bam-4823 55 24 slot slot NOUN bam-4823 55 25 for for ADP bam-4823 55 26 specimen speciman NOUN bam-4823 55 27 . . PUNCT bam-4823 56 1 c. c. NOUN bam-4823 56 2 trimming trim VERB bam-4823 56 3 a a DET bam-4823 56 4 specimen speciman NOUN bam-4823 56 5 carrier carrier NOUN bam-4823 56 6 in in ADP bam-4823 56 7 the the DET bam-4823 56 8 cryo cryo NOUN bam-4823 56 9 - - PUNCT bam-4823 56 10 ultramicrotome ultramicrotome NOUN bam-4823 56 11 . . PUNCT bam-4823 57 1 the the DET bam-4823 57 2 carrier carrier NOUN bam-4823 57 3 is be AUX bam-4823 57 4 trimmed trim VERB bam-4823 57 5 to to PART bam-4823 57 6 expose expose VERB bam-4823 57 7 the the DET bam-4823 57 8 specimen speciman NOUN bam-4823 57 9 at at ADP bam-4823 57 10 the the DET bam-4823 57 11 front front NOUN bam-4823 57 12 ( ( PUNCT bam-4823 57 13 i i NOUN bam-4823 57 14 , , PUNCT bam-4823 57 15 arrowhead arrowhead NOUN bam-4823 57 16 ) ) PUNCT bam-4823 57 17 and and CCONJ bam-4823 57 18 the the DET bam-4823 57 19 top top NOUN bam-4823 57 20 and and CCONJ bam-4823 57 21 bottom bottom ADJ bam-4823 57 22 ( ( PUNCT bam-4823 57 23 ii ii NOUN bam-4823 57 24 ) ) PUNCT bam-4823 57 25 ; ; PUNCT bam-4823 57 26 the the DET bam-4823 57 27 final final ADJ bam-4823 57 28 trimmed trim VERB bam-4823 57 29 specimen speciman NOUN bam-4823 57 30 is be AUX bam-4823 57 31 shaped shape VERB bam-4823 57 32 into into ADP bam-4823 57 33 a a DET bam-4823 57 34 1 1 NUM bam-4823 57 35 - - SYM bam-4823 57 36 2 2 NUM bam-4823 57 37 mm mm NOUN bam-4823 57 38 long long NOUN bam-4823 57 39 x x VERB bam-4823 57 40 20-µm 20-µm ADJ bam-4823 57 41 - - PUNCT bam-4823 57 42 thick thick ADJ bam-4823 57 43 edge edge NOUN bam-4823 57 44 ( ( PUNCT bam-4823 57 45 arrowheads arrowhead NOUN bam-4823 57 46 ) ) PUNCT bam-4823 57 47 , , PUNCT bam-4823 57 48 supported support VERB bam-4823 57 49 on on ADP bam-4823 57 50 the the DET bam-4823 57 51 edges edge NOUN bam-4823 57 52 by by ADP bam-4823 57 53 remaining remain VERB bam-4823 57 54 aluminum aluminum NOUN bam-4823 57 55 . . PUNCT bam-4823 58 1 d. d. PROPN bam-4823 58 2 custom custom PROPN bam-4823 58 3 specimen specimen PROPN bam-4823 58 4 block block VERB bam-4823 58 5 , , PUNCT bam-4823 58 6 shown show VERB bam-4823 58 7 with with ADP bam-4823 58 8 shutter shutter NOUN bam-4823 58 9 open open ADJ bam-4823 58 10 ( ( PUNCT bam-4823 58 11 left left ADJ bam-4823 58 12 ) ) PUNCT bam-4823 58 13 and and CCONJ bam-4823 58 14 closed closed ADJ bam-4823 58 15 ( ( PUNCT bam-4823 58 16 right right NOUN bam-4823 58 17 ) ) PUNCT bam-4823 58 18 . . PUNCT bam-4823 59 1 the the DET bam-4823 59 2 block block NOUN bam-4823 59 3 can can AUX bam-4823 59 4 hold hold VERB bam-4823 59 5 two two NUM bam-4823 59 6 intermediate intermediate ADJ bam-4823 59 7 specimen speciman NOUN bam-4823 59 8 holders holder NOUN bam-4823 59 9 or or CCONJ bam-4823 59 10 grids grid NOUN bam-4823 59 11 ( ( PUNCT bam-4823 59 12 inset inset NOUN bam-4823 59 13 , , PUNCT bam-4823 59 14 arrows arrow NOUN bam-4823 59 15 ) ) PUNCT bam-4823 59 16 . . PUNCT bam-4823 60 1 e. e. PROPN bam-4823 60 2 leica leica PROPN bam-4823 60 3 vct-100 vct-100 PROPN bam-4823 60 4 shuttle shuttle PROPN bam-4823 60 5 ready ready ADJ bam-4823 60 6 to to PART bam-4823 60 7 accept accept VERB bam-4823 60 8 the the DET bam-4823 60 9 specimen speciman NOUN bam-4823 60 10 block block NOUN bam-4823 60 11 ( ( PUNCT bam-4823 60 12 at at ADP bam-4823 60 13 right right NOUN bam-4823 60 14 ) ) PUNCT bam-4823 60 15 . . PUNCT bam-4823 61 1 f. f. PROPN bam-4823 61 2 sem sem PROPN bam-4823 61 3 of of ADP bam-4823 61 4 fib fib ADJ bam-4823 61 5 - - PUNCT bam-4823 61 6 milled mill VERB bam-4823 61 7 specimen speciman NOUN bam-4823 61 8 . . PUNCT bam-4823 62 1 edge edge NOUN bam-4823 62 2 - - PUNCT bam-4823 62 3 on on ADP bam-4823 62 4 view view NOUN bam-4823 62 5 ( ( PUNCT bam-4823 62 6 i i NOUN bam-4823 62 7 , , PUNCT bam-4823 62 8 similar similar ADJ bam-4823 62 9 to to PART bam-4823 62 10 view view VERB bam-4823 62 11 in in ADP bam-4823 62 12 cii cii PROPN bam-4823 62 13 ) ) PUNCT bam-4823 62 14 . . PUNCT bam-4823 63 1 ( ( PUNCT bam-4823 63 2 ii ii NOUN bam-4823 63 3 ) ) PUNCT bam-4823 63 4 ; ; PUNCT bam-4823 63 5 view view NOUN bam-4823 63 6 ( ( PUNCT bam-4823 63 7 perpendicular perpendicular ADJ bam-4823 63 8 to to ADP bam-4823 63 9 i i PRON bam-4823 63 10 ) ) PUNCT bam-4823 63 11 , , PUNCT bam-4823 63 12 showing show VERB bam-4823 63 13 the the DET bam-4823 63 14 four four NUM bam-4823 63 15 thinned thin VERB bam-4823 63 16 regions region NOUN bam-4823 63 17 ( ( PUNCT bam-4823 63 18 100 100 NUM bam-4823 63 19 - - SYM bam-4823 63 20 330 330 NUM bam-4823 63 21 nm nm ADV bam-4823 63 22 thick thick ADJ bam-4823 63 23 x x INTJ bam-4823 63 24 10 10 NUM bam-4823 63 25 µm µm NOUN bam-4823 63 26 x x SYM bam-4823 63 27 20 20 NUM bam-4823 63 28 µm µm NOUN bam-4823 63 29 ) ) PUNCT bam-4823 63 30 appropriate appropriate ADJ bam-4823 63 31 for for ADP bam-4823 63 32 imaging imaging NOUN bam-4823 63 33 in in ADP bam-4823 63 34 tem tem PROPN bam-4823 63 35 . . PUNCT bam-4823 64 1 asterisks asterisk NOUN bam-4823 64 2 indicate indicate VERB bam-4823 64 3 the the DET bam-4823 64 4 four four NUM bam-4823 64 5 thinned thin VERB bam-4823 64 6 lamellae lamellae NOUN bam-4823 64 7 as as SCONJ bam-4823 64 8 indicated indicate VERB bam-4823 64 9 by by ADP bam-4823 64 10 arrowheads arrowhead NOUN bam-4823 64 11 in in ADP bam-4823 64 12 ( ( PUNCT bam-4823 64 13 i i NOUN bam-4823 64 14 ) ) PUNCT bam-4823 64 15 . . PUNCT bam-4823 65 1 ( ( PUNCT bam-4823 65 2 iii iii NOUN bam-4823 65 3 ) ) PUNCT bam-4823 65 4 , , PUNCT bam-4823 65 5 sem sem ADJ bam-4823 65 6 - - PUNCT bam-4823 65 7 fib fib ADJ bam-4823 65 8 instrument instrument NOUN bam-4823 65 9 ( ( PUNCT bam-4823 65 10 zeiss zeiss PROPN bam-4823 65 11 neon neon NOUN bam-4823 65 12 40 40 NUM bam-4823 65 13 esb esb NOUN bam-4823 65 14 ) ) PUNCT bam-4823 65 15 . . PUNCT bam-4823 66 1 g. g. PROPN bam-4823 66 2 tip tip PROPN bam-4823 66 3 of of ADP bam-4823 66 4 modified modified ADJ bam-4823 66 5 gatan gatan PROPN bam-4823 66 6 626 626 NUM bam-4823 66 7 cryo cryo NOUN bam-4823 66 8 - - PUNCT bam-4823 66 9 transfer transfer NOUN bam-4823 66 10 holder holder NOUN bam-4823 66 11 , , PUNCT bam-4823 66 12 shown show VERB bam-4823 66 13 with with ADP bam-4823 66 14 door door NOUN bam-4823 66 15 ( ( PUNCT bam-4823 66 16 arrowhead arrowhead NOUN bam-4823 66 17 ) ) PUNCT bam-4823 66 18 and and CCONJ bam-4823 66 19 shutter shutter NOUN bam-4823 66 20 ( ( PUNCT bam-4823 66 21 asterisk asterisk NOUN bam-4823 66 22 ) ) PUNCT bam-4823 66 23 open open ADJ bam-4823 66 24 to to PART bam-4823 66 25 reveal reveal VERB bam-4823 66 26 the the DET bam-4823 66 27 specimen speciman NOUN bam-4823 66 28 in in ADP bam-4823 66 29 the the DET bam-4823 66 30 intermediate intermediate ADJ bam-4823 66 31 specimen speciman NOUN bam-4823 66 32 holder holder NOUN bam-4823 66 33 ( ( PUNCT bam-4823 66 34 arrow arrow NOUN bam-4823 66 35 ) ) PUNCT bam-4823 66 36 . . PUNCT bam-4823 67 1 parts part NOUN bam-4823 67 2 of of ADP bam-4823 67 3 this this DET bam-4823 67 4 figure figure NOUN bam-4823 67 5 were be AUX bam-4823 67 6 adapted adapt VERB bam-4823 67 7 from from ADP bam-4823 67 8 reference reference NOUN bam-4823 67 9 . . PUNCT bam-4823 68 1 13 13 NUM bam-4823 68 2 skeletal skeletal ADJ bam-4823 68 3 muscle muscle NOUN bam-4823 68 4 triad triad VERB bam-4823 68 5 by by ADP bam-4823 68 6 fib fib NOUN bam-4823 68 7 milling milling NOUN bam-4823 68 8 and and CCONJ bam-4823 68 9 cryo cryo NOUN bam-4823 68 10 - - PUNCT bam-4823 68 11 et et PROPN bam-4823 68 12 eur eur PROPN bam-4823 68 13 j j PROPN bam-4823 68 14 transl transl PROPN bam-4823 68 15 myol myol PROPN bam-4823 68 16 basic basic ADJ bam-4823 68 17 appl appl NOUN bam-4823 68 18 myol myol NOUN bam-4823 68 19 2015 2015 NUM bam-4823 68 20 ; ; PUNCT bam-4823 68 21 25 25 NUM bam-4823 68 22 ( ( PUNCT bam-4823 68 23 1 1 NUM bam-4823 68 24 ): ): PUNCT bam-4823 68 25 49 49 NUM bam-4823 68 26 - - SYM bam-4823 68 27 56 56 NUM bam-4823 68 28 52 52 NUM bam-4823 68 29 usually usually ADV bam-4823 68 30 step step VERB bam-4823 68 31 1 1 NUM bam-4823 68 32 ( ( PUNCT bam-4823 68 33 high high ADJ bam-4823 68 34 - - PUNCT bam-4823 68 35 pressure pressure NOUN bam-4823 68 36 freezing freezing NOUN bam-4823 68 37 ) ) PUNCT bam-4823 68 38 , , PUNCT bam-4823 68 39 steps step NOUN bam-4823 68 40 2 2 NUM bam-4823 68 41 - - SYM bam-4823 68 42 3 3 NUM bam-4823 68 43 ( ( PUNCT bam-4823 68 44 cryo cryo NOUN bam-4823 68 45 - - PUNCT bam-4823 68 46 ultramicrotomy ultramicrotomy NOUN bam-4823 68 47 ) ) PUNCT bam-4823 68 48 , , PUNCT bam-4823 68 49 steps step VERB bam-4823 68 50 4 4 NUM bam-4823 68 51 - - SYM bam-4823 68 52 5 5 NUM bam-4823 68 53 ( ( PUNCT bam-4823 68 54 fib fib ADJ bam-4823 68 55 milling milling NOUN bam-4823 68 56 ) ) PUNCT bam-4823 68 57 , , PUNCT bam-4823 68 58 and and CCONJ bam-4823 68 59 step step VERB bam-4823 68 60 7 7 NUM bam-4823 68 61 ( ( PUNCT bam-4823 68 62 cryo cryo NOUN bam-4823 68 63 - - PUNCT bam-4823 68 64 et et NOUN bam-4823 68 65 ) ) PUNCT bam-4823 68 66 are be AUX bam-4823 68 67 carried carry VERB bam-4823 68 68 out out ADP bam-4823 68 69 on on ADP bam-4823 68 70 separate separate ADJ bam-4823 68 71 days day NOUN bam-4823 68 72 , , PUNCT bam-4823 68 73 with with ADP bam-4823 68 74 the the DET bam-4823 68 75 fib fib NOUN bam-4823 68 76 milling milling NOUN bam-4823 68 77 being be AUX bam-4823 68 78 the the DET bam-4823 68 79 rate rate NOUN bam-4823 68 80 - - PUNCT bam-4823 68 81 limiting limit VERB bam-4823 68 82 step step NOUN bam-4823 68 83 . . PUNCT bam-4823 69 1 fig fig NOUN bam-4823 69 2 2 2 NUM bam-4823 69 3 . . PUNCT bam-4823 70 1 triad triad NOUN bam-4823 70 2 junctions junction NOUN bam-4823 70 3 imaged image VERB bam-4823 70 4 by by ADP bam-4823 70 5 cryo cryo NOUN bam-4823 70 6 - - PUNCT bam-4823 70 7 electron electron NOUN bam-4823 70 8 microscopy microscopy NOUN bam-4823 70 9 of of ADP bam-4823 70 10 fib fib ADJ bam-4823 70 11 - - PUNCT bam-4823 70 12 milled mill VERB bam-4823 70 13 muscle muscle NOUN bam-4823 70 14 . . PUNCT bam-4823 71 1 a. a. NOUN bam-4823 71 2 overview overview NOUN bam-4823 71 3 of of ADP bam-4823 71 4 a a DET bam-4823 71 5 300 300 NUM bam-4823 71 6 - - PUNCT bam-4823 71 7 nm nm ADJ bam-4823 71 8 - - PUNCT bam-4823 71 9 thick thick ADJ bam-4823 71 10 longitudinal longitudinal ADJ bam-4823 71 11 section section NOUN bam-4823 71 12 showing show VERB bam-4823 71 13 five five NUM bam-4823 71 14 triad triad ADJ bam-4823 71 15 junctions junction NOUN bam-4823 71 16 from from ADP bam-4823 71 17 toadfish toadfish ADJ bam-4823 71 18 swimbladder swimbladder NOUN bam-4823 71 19 . . PUNCT bam-4823 72 1 b b X bam-4823 72 2 - - PUNCT bam-4823 72 3 d. d. PROPN bam-4823 72 4 highermagnification highermagnification PROPN bam-4823 72 5 images image NOUN bam-4823 72 6 of of ADP bam-4823 72 7 three three NUM bam-4823 72 8 triad triad ADJ bam-4823 72 9 junctions junction NOUN bam-4823 72 10 . . PUNCT bam-4823 73 1 abbreviations abbreviation NOUN bam-4823 73 2 : : PUNCT bam-4823 73 3 sr sr PROPN bam-4823 73 4 , , PUNCT bam-4823 73 5 sarcoplasmic sarcoplasmic NOUN bam-4823 73 6 reticulum reticulum NOUN bam-4823 73 7 ; ; PUNCT bam-4823 73 8 tt tt PROPN bam-4823 73 9 , , PUNCT bam-4823 73 10 t t PROPN bam-4823 73 11 - - PUNCT bam-4823 73 12 tubule tubule NOUN bam-4823 73 13 ; ; PUNCT bam-4823 73 14 ryr ryr NOUN bam-4823 73 15 , , PUNCT bam-4823 73 16 ryanodine ryanodine VERB bam-4823 73 17 receptor receptor NOUN bam-4823 73 18 . . PUNCT bam-4823 74 1 e e X bam-4823 74 2 - - PROPN bam-4823 74 3 f. f. ADJ bam-4823 74 4 triad triad ADJ bam-4823 74 5 junctions junction NOUN bam-4823 74 6 from from ADP bam-4823 74 7 zebrafish zebrafish ADJ bam-4823 74 8 skeletal skeletal ADJ bam-4823 74 9 muscle muscle NOUN bam-4823 74 10 . . PUNCT bam-4823 75 1 e e PROPN bam-4823 75 2 shows show VERB bam-4823 75 3 a a DET bam-4823 75 4 longitudinal longitudinal ADJ bam-4823 75 5 view view NOUN bam-4823 75 6 with with ADP bam-4823 75 7 the the DET bam-4823 75 8 t t NOUN bam-4823 75 9 - - PUNCT bam-4823 75 10 tubule tubule NOUN bam-4823 75 11 in in ADP bam-4823 75 12 cross cross NOUN bam-4823 75 13 - - NOUN bam-4823 75 14 section section NOUN bam-4823 75 15 ( ( PUNCT bam-4823 75 16 as as ADP bam-4823 75 17 in in ADP bam-4823 75 18 a a DET bam-4823 75 19 - - PUNCT bam-4823 75 20 d d NOUN bam-4823 75 21 ) ) PUNCT bam-4823 75 22 , , PUNCT bam-4823 75 23 while while SCONJ bam-4823 75 24 f f PROPN bam-4823 75 25 shows show VERB bam-4823 75 26 a a DET bam-4823 75 27 longitudinal longitudinal ADJ bam-4823 75 28 view view NOUN bam-4823 75 29 in in ADP bam-4823 75 30 which which PRON bam-4823 75 31 the the DET bam-4823 75 32 t t NOUN bam-4823 75 33 - - PUNCT bam-4823 75 34 tubule tubule NOUN bam-4823 75 35 is be AUX bam-4823 75 36 viewed view VERB bam-4823 75 37 from from ADP bam-4823 75 38 the the DET bam-4823 75 39 side side NOUN bam-4823 75 40 ( ( PUNCT bam-4823 75 41 arrows arrow NOUN bam-4823 75 42 adjacent adjacent ADJ bam-4823 75 43 to to ADP bam-4823 75 44 “ " PUNCT bam-4823 75 45 tt tt PROPN bam-4823 75 46 ” " PUNCT bam-4823 75 47 indicate indicate VERB bam-4823 75 48 the the DET bam-4823 75 49 two two NUM bam-4823 75 50 opposing opposing ADJ bam-4823 75 51 surfaces surface NOUN bam-4823 75 52 of of ADP bam-4823 75 53 the the DET bam-4823 75 54 membrane membrane NOUN bam-4823 75 55 of of ADP bam-4823 75 56 the the DET bam-4823 75 57 t t NOUN bam-4823 75 58 - - PUNCT bam-4823 75 59 tubule tubule NOUN bam-4823 75 60 ) ) PUNCT bam-4823 75 61 ; ; PUNCT bam-4823 75 62 rows row NOUN bam-4823 75 63 of of ADP bam-4823 75 64 ryrs ryr NOUN bam-4823 75 65 are be AUX bam-4823 75 66 visible visible ADJ bam-4823 75 67 in in ADP bam-4823 75 68 the the DET bam-4823 75 69 two two NUM bam-4823 75 70 spaces space NOUN bam-4823 75 71 between between ADP bam-4823 75 72 the the DET bam-4823 75 73 tt tt PROPN bam-4823 75 74 and and CCONJ bam-4823 75 75 sr sr PROPN bam-4823 75 76 membranes membrane NOUN bam-4823 75 77 . . PUNCT bam-4823 76 1 a a PRON bam-4823 76 2 and and CCONJ bam-4823 76 3 b b NOUN bam-4823 76 4 adapted adapt VERB bam-4823 76 5 from from ADP bam-4823 76 6 reference reference NOUN bam-4823 76 7 . . PUNCT bam-4823 77 1 13 13 NUM bam-4823 77 2 skeletal skeletal ADJ bam-4823 77 3 muscle muscle NOUN bam-4823 77 4 triad triad VERB bam-4823 77 5 by by ADP bam-4823 77 6 fib fib NOUN bam-4823 77 7 milling milling NOUN bam-4823 77 8 and and CCONJ bam-4823 77 9 cryo cryo NOUN bam-4823 77 10 - - PUNCT bam-4823 77 11 et et PROPN bam-4823 77 12 eur eur PROPN bam-4823 77 13 j j PROPN bam-4823 77 14 transl transl PROPN bam-4823 77 15 myol myol PROPN bam-4823 77 16 basic basic ADJ bam-4823 77 17 appl appl NOUN bam-4823 77 18 myol myol NOUN bam-4823 77 19 2015 2015 NUM bam-4823 77 20 ; ; PUNCT bam-4823 77 21 25 25 NUM bam-4823 77 22 ( ( PUNCT bam-4823 77 23 1 1 NUM bam-4823 77 24 ): ): PUNCT bam-4823 77 25 49 49 NUM bam-4823 77 26 - - SYM bam-4823 77 27 56 56 NUM bam-4823 77 28 53 53 NUM bam-4823 77 29 ( ( PUNCT bam-4823 77 30 1 1 NUM bam-4823 77 31 ) ) PUNCT bam-4823 77 32 high high ADJ bam-4823 77 33 pressure pressure NOUN bam-4823 77 34 freezing freezing NOUN bam-4823 77 35 ( ( PUNCT bam-4823 77 36 fig fig NOUN bam-4823 77 37 . . PUNCT bam-4823 77 38 1a 1a NOUN bam-4823 77 39 ) ) PUNCT bam-4823 77 40 . . PUNCT bam-4823 78 1 deposit deposit NOUN bam-4823 78 2 fresh fresh ADJ bam-4823 78 3 tissue tissue NOUN bam-4823 78 4 in in ADP bam-4823 78 5 the the DET bam-4823 78 6 slot slot NOUN bam-4823 78 7 ( ( PUNCT bam-4823 78 8 e.g. e.g. ADV bam-4823 78 9 0.3 0.3 NUM bam-4823 78 10 mm mm NOUN bam-4823 78 11 wide wide ADJ bam-4823 78 12 x x SYM bam-4823 78 13 2 2 NUM bam-4823 78 14 mm mm NOUN bam-4823 78 15 long long ADJ bam-4823 78 16 ) ) PUNCT bam-4823 78 17 of of ADP bam-4823 78 18 a a DET bam-4823 78 19 standard standard ADJ bam-4823 78 20 hpf hpf NOUN bam-4823 78 21 specimen speciman NOUN bam-4823 78 22 carrier carrier NOUN bam-4823 78 23 ( ( PUNCT bam-4823 78 24 fig fig NOUN bam-4823 78 25 . . PUNCT bam-4823 79 1 1ai 1ai ADJ bam-4823 79 2 ) ) PUNCT bam-4823 79 3 and and CCONJ bam-4823 79 4 freeze freeze VERB bam-4823 79 5 . . PUNCT bam-4823 80 1 typically typically ADV bam-4823 80 2 , , PUNCT bam-4823 80 3 several several ADJ bam-4823 80 4 are be AUX bam-4823 80 5 prepared prepare VERB bam-4823 80 6 in in ADP bam-4823 80 7 a a DET bam-4823 80 8 session session NOUN bam-4823 80 9 , , PUNCT bam-4823 80 10 and and CCONJ bam-4823 80 11 these these PRON bam-4823 80 12 can can AUX bam-4823 80 13 be be AUX bam-4823 80 14 stored store VERB bam-4823 80 15 under under ADP bam-4823 80 16 liquid liquid ADJ bam-4823 80 17 nitrogen nitrogen NOUN bam-4823 80 18 indefinitely indefinitely ADV bam-4823 80 19 . . PUNCT bam-4823 81 1 ( ( PUNCT bam-4823 81 2 2 2 X bam-4823 81 3 ) ) PUNCT bam-4823 81 4 load load VERB bam-4823 81 5 the the DET bam-4823 81 6 specimen speciman NOUN bam-4823 81 7 carrier carrier NOUN bam-4823 81 8 into into ADP bam-4823 81 9 the the DET bam-4823 81 10 intermediate intermediate ADJ bam-4823 81 11 specimen speciman NOUN bam-4823 81 12 holder holder NOUN bam-4823 81 13 ( ( PUNCT bam-4823 81 14 ish ish PROPN bam-4823 81 15 ) ) PUNCT bam-4823 81 16 . . PUNCT bam-4823 82 1 the the DET bam-4823 82 2 custom custom ADV bam-4823 82 3 - - PUNCT bam-4823 82 4 made make VERB bam-4823 82 5 intermediate intermediate ADJ bam-4823 82 6 specimen speciman NOUN bam-4823 82 7 holder holder NOUN bam-4823 82 8 ( ( PUNCT bam-4823 82 9 fig fig NOUN bam-4823 82 10 . . PUNCT bam-4823 82 11 1b 1b NUM bam-4823 82 12 ) ) PUNCT bam-4823 82 13 allows allow VERB bam-4823 82 14 the the DET bam-4823 82 15 hpf hpf PROPN bam-4823 82 16 specimen speciman NOUN bam-4823 82 17 carrier carrier NOUN bam-4823 82 18 to to PART bam-4823 82 19 be be AUX bam-4823 82 20 conveniently conveniently ADV bam-4823 82 21 manipulated manipulate VERB bam-4823 82 22 throughout throughout ADP bam-4823 82 23 the the DET bam-4823 82 24 following follow VERB bam-4823 82 25 steps step NOUN bam-4823 82 26 . . PUNCT bam-4823 83 1 this this DET bam-4823 83 2 step step NOUN bam-4823 83 3 is be AUX bam-4823 83 4 typically typically ADV bam-4823 83 5 performed perform VERB bam-4823 83 6 in in ADP bam-4823 83 7 the the DET bam-4823 83 8 working work VERB bam-4823 83 9 chamber chamber NOUN bam-4823 83 10 of of ADP bam-4823 83 11 the the DET bam-4823 83 12 cryoultramicrotome cryoultramicrotome NOUN bam-4823 83 13 immediately immediately ADV bam-4823 83 14 before before ADP bam-4823 83 15 carrying carry VERB bam-4823 83 16 out out ADP bam-4823 83 17 the the DET bam-4823 83 18 next next ADJ bam-4823 83 19 step step NOUN bam-4823 83 20 . . PUNCT bam-4823 84 1 ( ( PUNCT bam-4823 84 2 3 3 X bam-4823 84 3 ) ) PUNCT bam-4823 84 4 pre pre ADJ bam-4823 84 5 - - VERB bam-4823 84 6 trim trim VERB bam-4823 84 7 the the DET bam-4823 84 8 intermediate intermediate ADJ bam-4823 84 9 specimen speciman NOUN bam-4823 84 10 holder holder NOUN bam-4823 84 11 and and CCONJ bam-4823 84 12 frozen frozen ADJ bam-4823 84 13 tissue tissue NOUN bam-4823 84 14 in in ADP bam-4823 84 15 a a DET bam-4823 84 16 cryo cryo NOUN bam-4823 84 17 - - PUNCT bam-4823 84 18 ultramicrotome ultramicrotome NOUN bam-4823 84 19 ( ( PUNCT bam-4823 84 20 fig fig NOUN bam-4823 84 21 . . PUNCT bam-4823 85 1 1c 1c NOUN bam-4823 85 2 ) ) PUNCT bam-4823 85 3 . . PUNCT bam-4823 86 1 most most ADJ bam-4823 86 2 of of ADP bam-4823 86 3 the the DET bam-4823 86 4 aluminum aluminum NOUN bam-4823 86 5 specimen speciman NOUN bam-4823 86 6 holder holder NOUN bam-4823 86 7 is be AUX bam-4823 86 8 trimmed trim VERB bam-4823 86 9 away away ADV bam-4823 86 10 , , PUNCT bam-4823 86 11 and and CCONJ bam-4823 86 12 the the DET bam-4823 86 13 specimen speciman NOUN bam-4823 86 14 is be AUX bam-4823 86 15 reduced reduce VERB bam-4823 86 16 to to ADP bam-4823 86 17 20 20 NUM bam-4823 86 18 - - SYM bam-4823 86 19 30 30 NUM bam-4823 86 20 µm µm NOUN bam-4823 86 21 in in ADP bam-4823 86 22 thickness thickness NOUN bam-4823 86 23 ( ( PUNCT bam-4823 86 24 fig fig NOUN bam-4823 86 25 . . PUNCT bam-4823 87 1 1cii 1cii NUM bam-4823 87 2 ) ) PUNCT bam-4823 87 3 . . PUNCT bam-4823 88 1 the the DET bam-4823 88 2 aluminum aluminum NOUN bam-4823 88 3 backing backing NOUN bam-4823 88 4 is be AUX bam-4823 88 5 removed remove VERB bam-4823 88 6 from from ADP bam-4823 88 7 the the DET bam-4823 88 8 bottom bottom ADJ bam-4823 88 9 surface surface NOUN bam-4823 88 10 of of ADP bam-4823 88 11 the the DET bam-4823 88 12 specimenbearing specimenbearing NOUN bam-4823 88 13 slot slot NOUN bam-4823 88 14 such such ADJ bam-4823 88 15 that that SCONJ bam-4823 88 16 the the DET bam-4823 88 17 thinned thin VERB bam-4823 88 18 specimen speciman NOUN bam-4823 88 19 area area NOUN bam-4823 88 20 is be AUX bam-4823 88 21 surrounded surround VERB bam-4823 88 22 by by ADP bam-4823 88 23 metal metal NOUN bam-4823 88 24 only only ADV bam-4823 88 25 on on ADP bam-4823 88 26 its its PRON bam-4823 88 27 sides side NOUN bam-4823 88 28 . . PUNCT bam-4823 89 1 milling milling NOUN bam-4823 89 2 will will AUX bam-4823 89 3 be be AUX bam-4823 89 4 initiated initiate VERB bam-4823 89 5 ( ( PUNCT bam-4823 89 6 step step NOUN bam-4823 89 7 6 6 NUM bam-4823 89 8 ) ) PUNCT bam-4823 89 9 at at ADP bam-4823 89 10 the the DET bam-4823 89 11 exposed expose VERB bam-4823 89 12 edge edge NOUN bam-4823 89 13 of of ADP bam-4823 89 14 specimen speciman NOUN bam-4823 89 15 . . PUNCT bam-4823 90 1 ( ( PUNCT bam-4823 90 2 4 4 X bam-4823 90 3 ) ) PUNCT bam-4823 90 4 transfer transfer NOUN bam-4823 90 5 the the DET bam-4823 90 6 ish ish NOUN bam-4823 90 7 containing contain VERB bam-4823 90 8 the the DET bam-4823 90 9 trimmed trim VERB bam-4823 90 10 specimen speciman NOUN bam-4823 90 11 to to ADP bam-4823 90 12 the the DET bam-4823 90 13 cryo cryo NOUN bam-4823 90 14 - - PUNCT bam-4823 90 15 sem sem NOUN bam-4823 90 16 specimen speciman NOUN bam-4823 90 17 block block VERB bam-4823 90 18 . . PUNCT bam-4823 91 1 the the DET bam-4823 91 2 block block NOUN bam-4823 91 3 ( ( PUNCT bam-4823 91 4 fig fig NOUN bam-4823 91 5 . . PUNCT bam-4823 92 1 1d 1d NUM bam-4823 92 2 ) ) PUNCT bam-4823 92 3 holds hold VERB bam-4823 92 4 and and CCONJ bam-4823 92 5 orients orient VERB bam-4823 92 6 the the DET bam-4823 92 7 specimen speciman NOUN bam-4823 92 8 in in ADP bam-4823 92 9 the the DET bam-4823 92 10 fib fib NOUN bam-4823 92 11 instrument instrument NOUN bam-4823 92 12 . . PUNCT bam-4823 93 1 this this DET bam-4823 93 2 block block NOUN bam-4823 93 3 is be AUX bam-4823 93 4 a a DET bam-4823 93 5 custom custom NOUN bam-4823 93 6 component component NOUN bam-4823 93 7 of of ADP bam-4823 93 8 the the DET bam-4823 93 9 leica leica PROPN bam-4823 93 10 vct-100 vct-100 PROPN bam-4823 93 11 cryo cryo NOUN bam-4823 93 12 - - PUNCT bam-4823 93 13 transfer transfer NOUN bam-4823 93 14 system system NOUN bam-4823 93 15 , , PUNCT bam-4823 93 16 which which PRON bam-4823 93 17 includes include VERB bam-4823 93 18 a a DET bam-4823 93 19 cryostage cryostage NOUN bam-4823 93 20 for for ADP bam-4823 93 21 the the DET bam-4823 93 22 fib fib ADJ bam-4823 93 23 - - PUNCT bam-4823 93 24 sem sem NOUN bam-4823 93 25 instrument instrument NOUN bam-4823 93 26 and and CCONJ bam-4823 93 27 a a DET bam-4823 93 28 cryotransfer cryotransfer NOUN bam-4823 93 29 shuttle shuttle NOUN bam-4823 93 30 system system NOUN bam-4823 93 31 . . PUNCT bam-4823 94 1 a a DET bam-4823 94 2 shutter shutter NOUN bam-4823 94 3 on on ADP bam-4823 94 4 the the DET bam-4823 94 5 specimen speciman NOUN bam-4823 94 6 block block NOUN bam-4823 94 7 protects protect VERB bam-4823 94 8 the the DET bam-4823 94 9 specimen speciman NOUN bam-4823 94 10 from from ADP bam-4823 94 11 contamination contamination NOUN bam-4823 94 12 during during ADP bam-4823 94 13 cryo cryo NOUN bam-4823 94 14 - - PUNCT bam-4823 94 15 transfer transfer NOUN bam-4823 94 16 . . PUNCT bam-4823 95 1 ( ( PUNCT bam-4823 95 2 5 5 X bam-4823 95 3 ) ) PUNCT bam-4823 95 4 transfer transfer NOUN bam-4823 95 5 the the DET bam-4823 95 6 specimen speciman NOUN bam-4823 95 7 block block VERB bam-4823 95 8 to to ADP bam-4823 95 9 the the DET bam-4823 95 10 fib fib ADJ bam-4823 95 11 instrument instrument NOUN bam-4823 95 12 . . PUNCT bam-4823 96 1 the the DET bam-4823 96 2 specimen speciman NOUN bam-4823 96 3 block block NOUN bam-4823 96 4 is be AUX bam-4823 96 5 inserted insert VERB bam-4823 96 6 into into ADP bam-4823 96 7 the the DET bam-4823 96 8 cryo cryo NOUN bam-4823 96 9 - - PUNCT bam-4823 96 10 transfer transfer NOUN bam-4823 96 11 shuttle shuttle NOUN bam-4823 96 12 ( ( PUNCT bam-4823 96 13 fig fig NOUN bam-4823 96 14 . . PUNCT bam-4823 97 1 1e 1e NUM bam-4823 97 2 ) ) PUNCT bam-4823 97 3 . . PUNCT bam-4823 98 1 the the DET bam-4823 98 2 shuttle shuttle NOUN bam-4823 98 3 docks dock VERB bam-4823 98 4 onto onto ADP bam-4823 98 5 the the DET bam-4823 98 6 fib fib ADJ bam-4823 98 7 - - PUNCT bam-4823 98 8 sem sem NOUN bam-4823 98 9 instrument instrument NOUN bam-4823 98 10 ( ( PUNCT bam-4823 98 11 fig fig NOUN bam-4823 98 12 . . PUNCT bam-4823 98 13 1fiii 1fiii NUM bam-4823 98 14 ) ) PUNCT bam-4823 98 15 and and CCONJ bam-4823 98 16 allows allow VERB bam-4823 98 17 the the DET bam-4823 98 18 specimen speciman NOUN bam-4823 98 19 block block VERB bam-4823 98 20 to to PART bam-4823 98 21 be be AUX bam-4823 98 22 moved move VERB bam-4823 98 23 to to ADP bam-4823 98 24 the the DET bam-4823 98 25 fib fib ADJ bam-4823 98 26 - - PUNCT bam-4823 98 27 milling mill VERB bam-4823 98 28 cryostage cryostage NOUN bam-4823 98 29 . . PUNCT bam-4823 99 1 the the DET bam-4823 99 2 shuttle shuttle NOUN bam-4823 99 3 also also ADV bam-4823 99 4 interfaces interface VERB bam-4823 99 5 with with ADP bam-4823 99 6 a a DET bam-4823 99 7 vacuum vacuum NOUN bam-4823 99 8 evaporator evaporator NOUN bam-4823 99 9 that that PRON bam-4823 99 10 allows allow VERB bam-4823 99 11 it it PRON bam-4823 99 12 to to PART bam-4823 99 13 be be AUX bam-4823 99 14 evacuated evacuate VERB bam-4823 99 15 before before ADP bam-4823 99 16 use use NOUN bam-4823 99 17 to to PART bam-4823 99 18 reduce reduce VERB bam-4823 99 19 exposure exposure NOUN bam-4823 99 20 of of ADP bam-4823 99 21 the the DET bam-4823 99 22 shuttle shuttle NOUN bam-4823 99 23 to to PART bam-4823 99 24 water water NOUN bam-4823 99 25 vapor vapor NOUN bam-4823 99 26 . . PUNCT bam-4823 100 1 loading loading NOUN bam-4823 100 2 ( ( PUNCT bam-4823 100 3 and and CCONJ bam-4823 100 4 unloading unloading NOUN bam-4823 100 5 ) ) PUNCT bam-4823 100 6 of of ADP bam-4823 100 7 the the DET bam-4823 100 8 specimen speciman NOUN bam-4823 100 9 block block VERB bam-4823 100 10 into into ADP bam-4823 100 11 the the DET bam-4823 100 12 shuttle shuttle NOUN bam-4823 100 13 is be AUX bam-4823 100 14 done do VERB bam-4823 100 15 under under ADP bam-4823 100 16 liquid liquid ADJ bam-4823 100 17 nitrogen nitrogen NOUN bam-4823 100 18 in in ADP bam-4823 100 19 a a DET bam-4823 100 20 loading loading NOUN bam-4823 100 21 box box NOUN bam-4823 100 22 . . PUNCT bam-4823 101 1 ( ( PUNCT bam-4823 101 2 6 6 NUM bam-4823 101 3 ) ) PUNCT bam-4823 101 4 fib fib NOUN bam-4823 101 5 milling milling NOUN bam-4823 101 6 . . PUNCT bam-4823 102 1 the the DET bam-4823 102 2 thin thin ADJ bam-4823 102 3 ( ( PUNCT bam-4823 102 4 20 20 NUM bam-4823 102 5 µm µm NOUN bam-4823 102 6 ) ) PUNCT bam-4823 102 7 platform platform NOUN bam-4823 102 8 of of ADP bam-4823 102 9 frozen frozen ADJ bam-4823 102 10 specimen speciman NOUN bam-4823 102 11 ( ( PUNCT bam-4823 102 12 300 300 NUM bam-4823 102 13 µm µm ADP bam-4823 102 14 w w NOUN bam-4823 102 15 x x PROPN bam-4823 102 16 500 500 NUM bam-4823 102 17 µm µm ADP bam-4823 102 18 l l NOUN bam-4823 102 19 ) ) PUNCT bam-4823 102 20 that that PRON bam-4823 102 21 was be AUX bam-4823 102 22 created create VERB bam-4823 102 23 in in ADP bam-4823 102 24 the the DET bam-4823 102 25 cryo cryo NOUN bam-4823 102 26 - - PUNCT bam-4823 102 27 ultramicrotome ultramicrotome NOUN bam-4823 102 28 ( ( PUNCT bam-4823 102 29 fig fig NOUN bam-4823 102 30 . . PUNCT bam-4823 102 31 1c 1c NOUN bam-4823 102 32 ) ) PUNCT bam-4823 102 33 is be AUX bam-4823 102 34 milled mill VERB bam-4823 102 35 in in ADP bam-4823 102 36 selected select VERB bam-4823 102 37 rectangular rectangular ADJ bam-4823 102 38 areas area NOUN bam-4823 102 39 ( ( PUNCT bam-4823 102 40 fig fig NOUN bam-4823 102 41 . . PUNCT bam-4823 103 1 1f 1f NUM bam-4823 103 2 ; ; PUNCT bam-4823 103 3 ̴ ̴ PROPN bam-4823 103 4 10 10 NUM bam-4823 103 5 µm µm PART bam-4823 103 6 w w PROPN bam-4823 103 7 x x PROPN bam-4823 103 8 20 20 NUM bam-4823 103 9 µm µm ADP bam-4823 103 10 l l NOUN bam-4823 103 11 ) ) PUNCT bam-4823 104 1 to to ADP bam-4823 104 2 the the DET bam-4823 104 3 desired desire VERB bam-4823 104 4 thickness thickness NOUN bam-4823 104 5 , , PUNCT bam-4823 104 6 typically typically ADV bam-4823 104 7 100300 100300 NUM bam-4823 104 8 nm nm NOUN bam-4823 104 9 . . PUNCT bam-4823 105 1 each each DET bam-4823 105 2 rectangular rectangular ADJ bam-4823 105 3 milled mill VERB bam-4823 105 4 area area NOUN bam-4823 105 5 is be AUX bam-4823 105 6 separated separate VERB bam-4823 105 7 from from ADP bam-4823 105 8 its its PRON bam-4823 105 9 neighbor(s neighbor(s NOUN bam-4823 105 10 ) ) PUNCT bam-4823 105 11 by by ADP bam-4823 105 12 regions region NOUN bam-4823 105 13 of of ADP bam-4823 105 14 thicker thick ADJ bam-4823 105 15 frozen frozen ADJ bam-4823 105 16 specimen speciman NOUN bam-4823 105 17 to to PART bam-4823 105 18 provide provide VERB bam-4823 105 19 mechanical mechanical ADJ bam-4823 105 20 stability stability NOUN bam-4823 105 21 , , PUNCT bam-4823 105 22 and and CCONJ bam-4823 105 23 the the DET bam-4823 105 24 edges edge NOUN bam-4823 105 25 are be AUX bam-4823 105 26 beveled bevel VERB bam-4823 105 27 to to PART bam-4823 105 28 allow allow VERB bam-4823 105 29 tilting tilting NOUN bam-4823 105 30 of of ADP bam-4823 105 31 the the DET bam-4823 105 32 specimen speciman NOUN bam-4823 105 33 to to ADP bam-4823 105 34 at at ADV bam-4823 105 35 least least ADV bam-4823 105 36 60 60 NUM bam-4823 105 37 degrees degree NOUN bam-4823 105 38 in in ADP bam-4823 105 39 the the DET bam-4823 105 40 tem tem PROPN bam-4823 105 41 . . PUNCT bam-4823 106 1 the the DET bam-4823 106 2 milling milling NOUN bam-4823 106 3 process process NOUN bam-4823 106 4 is be AUX bam-4823 106 5 done do VERB bam-4823 106 6 in in ADP bam-4823 106 7 several several ADJ bam-4823 106 8 steps step NOUN bam-4823 106 9 using use VERB bam-4823 106 10 a a DET bam-4823 106 11 higher high ADJ bam-4823 106 12 beam beam NOUN bam-4823 106 13 current current ADJ bam-4823 106 14 ( ( PUNCT bam-4823 106 15 e.g. e.g. ADV bam-4823 106 16 5 5 NUM bam-4823 106 17 na na NOUN bam-4823 106 18 ) ) PUNCT bam-4823 106 19 for for ADP bam-4823 106 20 the the DET bam-4823 106 21 initial initial ADJ bam-4823 106 22 steps step NOUN bam-4823 106 23 and and CCONJ bam-4823 106 24 a a DET bam-4823 106 25 lower low ADJ bam-4823 106 26 beam beam NOUN bam-4823 106 27 current current ADJ bam-4823 106 28 ( ( PUNCT bam-4823 106 29 e.g. e.g. ADV bam-4823 106 30 100 100 NUM bam-4823 106 31 pa pa NOUN bam-4823 106 32 ) ) PUNCT bam-4823 106 33 in in ADP bam-4823 106 34 the the DET bam-4823 106 35 final final ADJ bam-4823 106 36 step step NOUN bam-4823 106 37 . . PUNCT bam-4823 107 1 typically typically ADV bam-4823 107 2 we we PRON bam-4823 107 3 create create VERB bam-4823 107 4 four four NUM bam-4823 107 5 groups group NOUN bam-4823 107 6 of of ADP bam-4823 107 7 the the DET bam-4823 107 8 final final ADJ bam-4823 107 9 rectangular rectangular ADJ bam-4823 107 10 areas area NOUN bam-4823 107 11 that that PRON bam-4823 107 12 are be AUX bam-4823 107 13 suitable suitable ADJ bam-4823 107 14 for for ADP bam-4823 107 15 cryo cryo NOUN bam-4823 107 16 - - PUNCT bam-4823 107 17 tem tem PROPN bam-4823 107 18 imaging imaging NOUN bam-4823 107 19 . . PUNCT bam-4823 108 1 the the DET bam-4823 108 2 duration duration NOUN bam-4823 108 3 of of ADP bam-4823 108 4 the the DET bam-4823 108 5 actual actual ADJ bam-4823 108 6 milling milling NOUN bam-4823 108 7 is be AUX bam-4823 108 8 about about ADV bam-4823 108 9 one one NUM bam-4823 108 10 hour hour NOUN bam-4823 108 11 for for ADP bam-4823 108 12 two two NUM bam-4823 108 13 groups group NOUN bam-4823 108 14 of of ADP bam-4823 108 15 four four NUM bam-4823 108 16 . . PUNCT bam-4823 109 1 thus thus ADV bam-4823 109 2 , , PUNCT bam-4823 109 3 up up ADP bam-4823 109 4 to to PART bam-4823 109 5 16 16 NUM bam-4823 109 6 regions region NOUN bam-4823 109 7 suitable suitable ADJ bam-4823 109 8 for for ADP bam-4823 109 9 tem tem PROPN bam-4823 109 10 can can AUX bam-4823 109 11 be be AUX bam-4823 109 12 created create VERB bam-4823 109 13 from from ADP bam-4823 109 14 a a DET bam-4823 109 15 single single ADJ bam-4823 109 16 specimen speciman NOUN bam-4823 109 17 carrier carrier NOUN bam-4823 109 18 in in ADP bam-4823 109 19 one one NUM bam-4823 109 20 several several ADJ bam-4823 109 21 - - PUNCT bam-4823 109 22 hour hour NOUN bam-4823 109 23 session session NOUN bam-4823 109 24 . . PUNCT bam-4823 110 1 upon upon SCONJ bam-4823 110 2 completion completion NOUN bam-4823 110 3 of of ADP bam-4823 110 4 milling milling NOUN bam-4823 110 5 , , PUNCT bam-4823 110 6 the the DET bam-4823 110 7 specimen speciman NOUN bam-4823 110 8 block block NOUN bam-4823 110 9 is be AUX bam-4823 110 10 removed remove VERB bam-4823 110 11 from from ADP bam-4823 110 12 the the DET bam-4823 110 13 fib fib ADJ bam-4823 110 14 - - PUNCT bam-4823 110 15 sem sem NOUN bam-4823 110 16 instrument instrument NOUN bam-4823 110 17 via via ADP bam-4823 110 18 the the DET bam-4823 110 19 shuttle shuttle NOUN bam-4823 110 20 , , PUNCT bam-4823 110 21 transferred transfer VERB bam-4823 110 22 from from ADP bam-4823 110 23 the the DET bam-4823 110 24 shuttle shuttle NOUN bam-4823 110 25 to to ADP bam-4823 110 26 the the DET bam-4823 110 27 loading loading NOUN bam-4823 110 28 box box NOUN bam-4823 110 29 , , PUNCT bam-4823 110 30 and and CCONJ bam-4823 110 31 then then ADV bam-4823 110 32 may may AUX bam-4823 110 33 be be AUX bam-4823 110 34 stored store VERB bam-4823 110 35 under under ADP bam-4823 110 36 liquid liquid ADJ bam-4823 110 37 nitrogen nitrogen NOUN bam-4823 110 38 until until SCONJ bam-4823 110 39 needed need VERB bam-4823 110 40 for for ADP bam-4823 110 41 cryo cryo NOUN bam-4823 110 42 - - PUNCT bam-4823 110 43 tem tem PROPN bam-4823 110 44 . . PUNCT bam-4823 111 1 ( ( PUNCT bam-4823 111 2 7 7 X bam-4823 111 3 ) ) PUNCT bam-4823 111 4 transfer transfer NOUN bam-4823 111 5 the the DET bam-4823 111 6 ish ish NOUN bam-4823 111 7 containing contain VERB bam-4823 111 8 the the DET bam-4823 111 9 milled mill VERB bam-4823 111 10 specimen speciman NOUN bam-4823 111 11 to to ADP bam-4823 111 12 the the DET bam-4823 111 13 tem tem PROPN bam-4823 111 14 cryo cryo NOUN bam-4823 111 15 - - PUNCT bam-4823 111 16 holder holder NOUN bam-4823 111 17 for for ADP bam-4823 111 18 cryo cryo NOUN bam-4823 111 19 - - PUNCT bam-4823 111 20 tem tem PROPN bam-4823 111 21 . . PUNCT bam-4823 112 1 the the DET bam-4823 112 2 specimen speciman NOUN bam-4823 112 3 block block VERB bam-4823 112 4 containing contain VERB bam-4823 112 5 the the DET bam-4823 112 6 fib fib ADJ bam-4823 112 7 - - PUNCT bam-4823 112 8 milled mill VERB bam-4823 112 9 specimen(s specimen(s NOUN bam-4823 112 10 ) ) PUNCT bam-4823 112 11 is be AUX bam-4823 112 12 transferred transfer VERB bam-4823 112 13 to to ADP bam-4823 112 14 the the DET bam-4823 112 15 workstation workstation NOUN bam-4823 112 16 that that PRON bam-4823 112 17 is be AUX bam-4823 112 18 used use VERB bam-4823 112 19 to to PART bam-4823 112 20 load load VERB bam-4823 112 21 the the DET bam-4823 112 22 tem tem PROPN bam-4823 112 23 cryo cryo NOUN bam-4823 112 24 - - PUNCT bam-4823 112 25 holder holder NOUN bam-4823 112 26 , , PUNCT bam-4823 112 27 and and CCONJ bam-4823 112 28 the the DET bam-4823 112 29 specimen speciman NOUN bam-4823 112 30 - - PUNCT bam-4823 112 31 loaded load VERB bam-4823 112 32 ish ish NOUN bam-4823 112 33 is be AUX bam-4823 112 34 transferred transfer VERB bam-4823 112 35 to to ADP bam-4823 112 36 the the DET bam-4823 112 37 cryo cryo NOUN bam-4823 112 38 - - PUNCT bam-4823 112 39 holder holder NOUN bam-4823 112 40 ( ( PUNCT bam-4823 112 41 fig fig NOUN bam-4823 112 42 . . PUNCT bam-4823 113 1 1 1 NUM bam-4823 113 2 g g NOUN bam-4823 113 3 ) ) PUNCT bam-4823 113 4 . . PUNCT bam-4823 114 1 we we PRON bam-4823 114 2 have have AUX bam-4823 114 3 modified modify VERB bam-4823 114 4 the the DET bam-4823 114 5 tip tip NOUN bam-4823 114 6 of of ADP bam-4823 114 7 a a DET bam-4823 114 8 gatan gatan PROPN bam-4823 114 9 model model NOUN bam-4823 114 10 626 626 NUM bam-4823 114 11 cryo cryo NOUN bam-4823 114 12 - - PUNCT bam-4823 114 13 holder holder NOUN bam-4823 114 14 ( ( PUNCT bam-4823 114 15 gatan gatan PROPN bam-4823 114 16 , , PUNCT bam-4823 114 17 inc inc PROPN bam-4823 114 18 . . PROPN bam-4823 114 19 , , PUNCT bam-4823 114 20 pleasanton pleasanton PROPN bam-4823 114 21 , , PUNCT bam-4823 114 22 ca ca NOUN bam-4823 114 23 ) ) PUNCT bam-4823 114 24 to to PART bam-4823 114 25 accept accept VERB bam-4823 114 26 the the DET bam-4823 114 27 ish ish NOUN bam-4823 114 28 instead instead ADV bam-4823 114 29 of of ADP bam-4823 114 30 the the DET bam-4823 114 31 usual usual ADJ bam-4823 114 32 em em PRON bam-4823 114 33 grid grid NOUN bam-4823 114 34 . . PUNCT bam-4823 115 1 cryo cryo PROPN bam-4823 115 2 - - PUNCT bam-4823 115 3 em em PROPN bam-4823 115 4 and and CCONJ bam-4823 115 5 collection collection NOUN bam-4823 115 6 of of ADP bam-4823 115 7 the the DET bam-4823 115 8 tilt tilt NOUN bam-4823 115 9 series series NOUN bam-4823 115 10 for for ADP bam-4823 115 11 tomography tomography NOUN bam-4823 115 12 is be AUX bam-4823 115 13 conducted conduct VERB bam-4823 115 14 according accord VERB bam-4823 115 15 to to ADP bam-4823 115 16 standard standard ADJ bam-4823 115 17 procedures procedure NOUN bam-4823 115 18 . . PUNCT bam-4823 116 1 cryo cryo PROPN bam-4823 116 2 - - PUNCT bam-4823 116 3 tem tem PROPN bam-4823 116 4 and and CCONJ bam-4823 116 5 cryo cryo NOUN bam-4823 116 6 - - PUNCT bam-4823 116 7 et et NOUN bam-4823 116 8 of of ADP bam-4823 116 9 fib fib ADJ bam-4823 116 10 - - PUNCT bam-4823 116 11 milled mill VERB bam-4823 116 12 skeletal skeletal ADJ bam-4823 116 13 muscle muscle NOUN bam-4823 116 14 . . PUNCT bam-4823 117 1 the the DET bam-4823 117 2 skeletal skeletal ADJ bam-4823 117 3 muscle muscle NOUN bam-4823 117 4 triad triad ADJ bam-4823 117 5 junction junction NOUN bam-4823 117 6 would would AUX bam-4823 117 7 appear appear VERB bam-4823 117 8 to to PART bam-4823 117 9 be be AUX bam-4823 117 10 well well ADV bam-4823 117 11 - - PUNCT bam-4823 117 12 suited suit VERB bam-4823 117 13 for for ADP bam-4823 117 14 application application NOUN bam-4823 117 15 of of ADP bam-4823 117 16 our our PRON bam-4823 117 17 method method NOUN bam-4823 117 18 of of ADP bam-4823 117 19 applying apply VERB bam-4823 117 20 fib fib NOUN bam-4823 117 21 milling milling NOUN bam-4823 117 22 to to ADP bam-4823 117 23 specimens specimen NOUN bam-4823 117 24 for for ADP bam-4823 117 25 cryo cryo NOUN bam-4823 117 26 - - PUNCT bam-4823 117 27 et et NOUN bam-4823 117 28 . . PUNCT bam-4823 118 1 firstly firstly ADV bam-4823 118 2 , , PUNCT bam-4823 118 3 from from ADP bam-4823 118 4 a a DET bam-4823 118 5 practical practical ADJ bam-4823 118 6 viewpoint viewpoint NOUN bam-4823 118 7 , , PUNCT bam-4823 118 8 muscle muscle NOUN bam-4823 118 9 is be AUX bam-4823 118 10 constructed construct VERB bam-4823 118 11 of of ADP bam-4823 118 12 repeating repeat VERB bam-4823 118 13 units unit NOUN bam-4823 118 14 ( ( PUNCT bam-4823 118 15 sarcomeres sarcomere NOUN bam-4823 118 16 ) ) PUNCT bam-4823 118 17 , , PUNCT bam-4823 118 18 thus thus ADV bam-4823 118 19 essentially essentially ADV bam-4823 118 20 any any DET bam-4823 118 21 electron electron NOUN bam-4823 118 22 micrograph micrograph NOUN bam-4823 118 23 of of ADP bam-4823 118 24 a a DET bam-4823 118 25 longitudinal longitudinal ADJ bam-4823 118 26 cryosection cryosection NOUN bam-4823 118 27 will will AUX bam-4823 118 28 contain contain VERB bam-4823 118 29 several several ADJ bam-4823 118 30 triad triad ADJ bam-4823 118 31 junctional junctional ADJ bam-4823 118 32 regions region NOUN bam-4823 118 33 . . PUNCT bam-4823 119 1 this this PRON bam-4823 119 2 is be AUX bam-4823 119 3 important important ADJ bam-4823 119 4 because because SCONJ bam-4823 119 5 one one NUM bam-4823 119 6 of of ADP bam-4823 119 7 the the DET bam-4823 119 8 current current ADJ bam-4823 119 9 limitations limitation NOUN bam-4823 119 10 of of ADP bam-4823 119 11 cryo cryo NOUN bam-4823 119 12 - - PUNCT bam-4823 119 13 et et NOUN bam-4823 119 14 as as SCONJ bam-4823 119 15 applied apply VERB bam-4823 119 16 to to ADP bam-4823 119 17 vitreous vitreous ADJ bam-4823 119 18 cryosections cryosection NOUN bam-4823 119 19 of of ADP bam-4823 119 20 eukaryotic eukaryotic ADJ bam-4823 119 21 cells cell NOUN bam-4823 119 22 is be AUX bam-4823 119 23 that that SCONJ bam-4823 119 24 a a DET bam-4823 119 25 substructure substructure NOUN bam-4823 119 26 of of ADP bam-4823 119 27 interest interest NOUN bam-4823 119 28 is be AUX bam-4823 119 29 usually usually ADV bam-4823 119 30 only only ADV bam-4823 119 31 detectable detectable ADJ bam-4823 119 32 after after SCONJ bam-4823 119 33 the the DET bam-4823 119 34 tomographic tomographic ADJ bam-4823 119 35 data datum NOUN bam-4823 119 36 is be AUX bam-4823 119 37 collected collect VERB bam-4823 119 38 . . PUNCT bam-4823 120 1 consequently consequently ADV bam-4823 120 2 , , PUNCT bam-4823 120 3 small small ADJ bam-4823 120 4 - - PUNCT bam-4823 120 5 copy copy NOUN bam-4823 120 6 - - PUNCT bam-4823 120 7 number number NOUN bam-4823 120 8 structures structure NOUN bam-4823 120 9 are be AUX bam-4823 120 10 likely likely ADJ bam-4823 120 11 not not PART bam-4823 120 12 to to PART bam-4823 120 13 be be AUX bam-4823 120 14 present present ADJ bam-4823 120 15 in in ADP bam-4823 120 16 the the DET bam-4823 120 17 reconstructed reconstructed ADJ bam-4823 120 18 volume volume NOUN bam-4823 120 19 , , PUNCT bam-4823 120 20 which which PRON bam-4823 120 21 typically typically ADV bam-4823 120 22 represents represent VERB bam-4823 120 23 a a DET bam-4823 120 24 tiny tiny ADJ bam-4823 120 25 fraction fraction NOUN bam-4823 120 26 of of ADP bam-4823 120 27 the the DET bam-4823 120 28 total total ADJ bam-4823 120 29 cell cell NOUN bam-4823 120 30 volume volume NOUN bam-4823 120 31 . . PUNCT bam-4823 121 1 secondly secondly ADV bam-4823 121 2 , , PUNCT bam-4823 121 3 from from ADP bam-4823 121 4 the the DET bam-4823 121 5 viewpoint viewpoint NOUN bam-4823 121 6 of of ADP bam-4823 121 7 structural structural ADJ bam-4823 121 8 biology biology NOUN bam-4823 121 9 , , PUNCT bam-4823 121 10 cryo cryo NOUN bam-4823 121 11 - - PUNCT bam-4823 121 12 et et PROPN bam-4823 121 13 has have VERB bam-4823 121 14 the the DET bam-4823 121 15 potential potential NOUN bam-4823 121 16 to to PART bam-4823 121 17 reveal reveal VERB bam-4823 121 18 details detail NOUN bam-4823 121 19 of of ADP bam-4823 121 20 the the DET bam-4823 121 21 triad triad ADJ bam-4823 121 22 junction junction NOUN bam-4823 121 23 at at ADP bam-4823 121 24 an an DET bam-4823 121 25 unprecedented unprecedented ADJ bam-4823 121 26 level level NOUN bam-4823 121 27 . . PUNCT bam-4823 122 1 although although SCONJ bam-4823 122 2 some some PRON bam-4823 122 3 of of ADP bam-4823 122 4 the the DET bam-4823 122 5 triad triad NOUN bam-4823 122 6 ’s ’s PART bam-4823 122 7 major major ADJ bam-4823 122 8 structural structural ADJ bam-4823 122 9 features feature NOUN bam-4823 122 10 , , PUNCT bam-4823 122 11 such such ADJ bam-4823 122 12 as as ADP bam-4823 122 13 the the DET bam-4823 122 14 spatial spatial ADJ bam-4823 122 15 distribution distribution NOUN bam-4823 122 16 of of ADP bam-4823 122 17 ryrs ryr NOUN bam-4823 122 18 in in ADP bam-4823 122 19 the the DET bam-4823 122 20 terminal terminal ADJ bam-4823 122 21 cisternae cisternae NOUN bam-4823 122 22 region region NOUN bam-4823 122 23 of of ADP bam-4823 122 24 the the DET bam-4823 122 25 sarcoplasmic sarcoplasmic NOUN bam-4823 122 26 reticulum reticulum NOUN bam-4823 122 27 and and CCONJ bam-4823 122 28 that that PRON bam-4823 122 29 of of ADP bam-4823 122 30 the the DET bam-4823 122 31 dhprs dhprs NOUN bam-4823 122 32 in in ADP bam-4823 122 33 the the DET bam-4823 122 34 apposed appose VERB bam-4823 122 35 transverse transverse NOUN bam-4823 122 36 tubule tubule NOUN bam-4823 122 37 , , PUNCT bam-4823 122 38 have have AUX bam-4823 122 39 been be AUX bam-4823 122 40 gleaned glean VERB bam-4823 122 41 from from ADP bam-4823 122 42 classical classical ADJ bam-4823 122 43 electron electron NOUN bam-4823 122 44 microscopy microscopy NOUN bam-4823 122 45 , , PUNCT bam-4823 122 46 the the DET bam-4823 122 47 molecular molecular ADJ bam-4823 122 48 details detail NOUN bam-4823 122 49 of of ADP bam-4823 122 50 the the DET bam-4823 122 51 interaction interaction NOUN bam-4823 122 52 between between ADP bam-4823 122 53 the the DET bam-4823 122 54 ryr ryr NOUN bam-4823 122 55 and and CCONJ bam-4823 122 56 the the DET bam-4823 122 57 dhprs dhprs NOUN bam-4823 122 58 remain remain VERB bam-4823 122 59 unknown unknown ADJ bam-4823 122 60 , , PUNCT bam-4823 122 61 as as SCONJ bam-4823 122 62 are be AUX bam-4823 122 63 the the DET bam-4823 122 64 locations location NOUN bam-4823 122 65 and and CCONJ bam-4823 122 66 intermolecular intermolecular ADJ bam-4823 122 67 interactions interaction NOUN bam-4823 122 68 among among ADP bam-4823 122 69 the the DET bam-4823 122 70 numerous numerous ADJ bam-4823 122 71 other other ADJ bam-4823 122 72 proteins protein NOUN bam-4823 122 73 that that PRON bam-4823 122 74 contribute contribute VERB bam-4823 122 75 to to ADP bam-4823 122 76 the the DET bam-4823 122 77 triad triad ADJ bam-4823 122 78 junction junction NOUN bam-4823 122 79 . . PUNCT bam-4823 123 1 figure figure NOUN bam-4823 123 2 2 2 NUM bam-4823 123 3 shows show VERB bam-4823 123 4 some some PRON bam-4823 123 5 of of ADP bam-4823 123 6 our our PRON bam-4823 123 7 initial initial ADJ bam-4823 123 8 micrographs micrograph NOUN bam-4823 123 9 from from ADP bam-4823 123 10 fib fib ADJ bam-4823 123 11 - - PUNCT bam-4823 123 12 milled mill VERB bam-4823 123 13 fast fast ADJ bam-4823 123 14 - - PUNCT bam-4823 123 15 twitch twitch NOUN bam-4823 123 16 toadfish toadfish ADJ bam-4823 123 17 swim swim NOUN bam-4823 123 18 bladder bladder NOUN bam-4823 123 19 , , PUNCT bam-4823 123 20 which which PRON bam-4823 123 21 has have AUX bam-4823 123 22 previously previously ADV bam-4823 123 23 been be AUX bam-4823 123 24 extensively extensively ADV bam-4823 123 25 characterized characterize VERB bam-4823 123 26 by by ADP bam-4823 123 27 tem tem PROPN bam-4823 123 28 of of ADP bam-4823 123 29 conventional conventional ADJ bam-4823 123 30 thin thin ADJ bam-4823 123 31 - - PUNCT bam-4823 123 32 section section NOUN bam-4823 123 33 and and CCONJ bam-4823 123 34 freeze freeze ADJ bam-4823 123 35 - - PUNCT bam-4823 123 36 fracture fracture NOUN bam-4823 123 37 specimens specimen NOUN bam-4823 123 38 by by ADP bam-4823 123 39 dr dr PROPN bam-4823 123 40 . . PROPN bam-4823 123 41 c. c. PROPN bam-4823 123 42 franzini franzini PROPN bam-4823 123 43 - - PUNCT bam-4823 123 44 armstrong armstrong PROPN bam-4823 123 45 , , PUNCT bam-4823 123 46 who who PRON bam-4823 123 47 assisted assist VERB bam-4823 123 48 us we PRON bam-4823 123 49 with with ADP bam-4823 123 50 the the DET bam-4823 123 51 preparation preparation NOUN bam-4823 123 52 of of ADP bam-4823 123 53 the the DET bam-4823 123 54 swimbladder swimbladder NOUN bam-4823 123 55 muscle muscle NOUN bam-4823 123 56 . . PUNCT bam-4823 124 1 24 24 NUM bam-4823 124 2 for for ADP bam-4823 124 3 convenience convenience NOUN bam-4823 124 4 , , PUNCT bam-4823 124 5 for for ADP bam-4823 124 6 our our PRON bam-4823 124 7 initial initial ADJ bam-4823 124 8 work work NOUN bam-4823 124 9 to to PART bam-4823 124 10 demonstrate demonstrate VERB bam-4823 124 11 feasibility feasibility NOUN bam-4823 124 12 of of ADP bam-4823 124 13 fib fib NOUN bam-4823 124 14 - - PUNCT bam-4823 124 15 milling milling NOUN bam-4823 124 16 for for ADP bam-4823 124 17 cryo cryo NOUN bam-4823 124 18 - - PUNCT bam-4823 124 19 et et PROPN bam-4823 124 20 , , PUNCT bam-4823 124 21 the the DET bam-4823 124 22 muscle muscle NOUN bam-4823 124 23 was be AUX bam-4823 124 24 fixed fix VERB bam-4823 124 25 with with ADP bam-4823 124 26 2.5 2.5 NUM bam-4823 124 27 % % NOUN bam-4823 124 28 glutaraldehyde glutaraldehyde NOUN bam-4823 124 29 , , PUNCT bam-4823 124 30 but but CCONJ bam-4823 124 31 the the DET bam-4823 124 32 specimens specimen NOUN bam-4823 124 33 remained remain VERB bam-4823 124 34 fully fully ADV bam-4823 124 35 hydrated hydrated ADJ bam-4823 124 36 and and CCONJ bam-4823 124 37 no no DET bam-4823 124 38 heavy heavy ADJ bam-4823 124 39 metal metal NOUN bam-4823 124 40 stains stain NOUN bam-4823 124 41 were be AUX bam-4823 124 42 used use VERB bam-4823 124 43 . . PUNCT bam-4823 125 1 in in ADP bam-4823 125 2 longitudinal longitudinal ADJ bam-4823 125 3 sections section NOUN bam-4823 125 4 , , PUNCT bam-4823 125 5 as as SCONJ bam-4823 125 6 expected expect VERB bam-4823 125 7 , , PUNCT bam-4823 125 8 triad triad VERB bam-4823 125 9 skeletal skeletal ADJ bam-4823 125 10 muscle muscle NOUN bam-4823 125 11 triad triad VERB bam-4823 125 12 by by ADP bam-4823 125 13 fib fib NOUN bam-4823 125 14 milling milling NOUN bam-4823 125 15 and and CCONJ bam-4823 125 16 cryo cryo NOUN bam-4823 125 17 - - PUNCT bam-4823 125 18 et et PROPN bam-4823 125 19 eur eur PROPN bam-4823 125 20 j j PROPN bam-4823 125 21 transl transl PROPN bam-4823 125 22 myol myol PROPN bam-4823 125 23 basic basic ADJ bam-4823 125 24 appl appl NOUN bam-4823 125 25 myol myol NOUN bam-4823 125 26 2015 2015 NUM bam-4823 125 27 ; ; PUNCT bam-4823 125 28 25 25 NUM bam-4823 125 29 ( ( PUNCT bam-4823 125 30 1 1 NUM bam-4823 125 31 ): ): PUNCT bam-4823 125 32 49 49 NUM bam-4823 125 33 - - SYM bam-4823 125 34 56 56 NUM bam-4823 125 35 54 54 NUM bam-4823 125 36 junctions junction NOUN bam-4823 125 37 are be AUX bam-4823 125 38 prevalent prevalent ADJ bam-4823 125 39 , , PUNCT bam-4823 125 40 and and CCONJ bam-4823 125 41 the the DET bam-4823 125 42 major major ADJ bam-4823 125 43 structural structural ADJ bam-4823 125 44 features feature NOUN bam-4823 125 45 agree agree VERB bam-4823 125 46 remarkably remarkably ADV bam-4823 125 47 well well ADV bam-4823 125 48 with with ADP bam-4823 125 49 those those PRON bam-4823 125 50 described describe VERB bam-4823 125 51 previously previously ADV bam-4823 125 52 , , PUNCT bam-4823 125 53 albeit albeit SCONJ bam-4823 125 54 at at ADP bam-4823 125 55 reduced reduced ADJ bam-4823 125 56 contrast contrast NOUN bam-4823 125 57 because because SCONJ bam-4823 125 58 we we PRON bam-4823 125 59 are be AUX bam-4823 125 60 imaging image VERB bam-4823 125 61 native native ADJ bam-4823 125 62 muscle muscle NOUN bam-4823 125 63 . . PUNCT bam-4823 126 1 in in ADP bam-4823 126 2 certain certain ADJ bam-4823 126 3 orientations orientation NOUN bam-4823 126 4 , , PUNCT bam-4823 126 5 the the DET bam-4823 126 6 ryanodine ryanodine NOUN bam-4823 126 7 receptors receptor NOUN bam-4823 126 8 , , PUNCT bam-4823 126 9 whose whose DET bam-4823 126 10 cytoplasmic cytoplasmic ADJ bam-4823 126 11 regions region NOUN bam-4823 126 12 ( ( PUNCT bam-4823 126 13 also also ADV bam-4823 126 14 known know VERB bam-4823 126 15 as as ADP bam-4823 126 16 foot foot NOUN bam-4823 126 17 structures structure NOUN bam-4823 126 18 ) ) PUNCT bam-4823 126 19 bridge bridge NOUN bam-4823 126 20 the the DET bam-4823 126 21 gap gap NOUN bam-4823 126 22 between between ADP bam-4823 126 23 the the DET bam-4823 126 24 junctional junctional ADJ bam-4823 126 25 sr sr PROPN bam-4823 126 26 membrane membrane NOUN bam-4823 126 27 and and CCONJ bam-4823 126 28 the the DET bam-4823 126 29 t t NOUN bam-4823 126 30 - - PUNCT bam-4823 126 31 tubule tubule NOUN bam-4823 126 32 , , PUNCT bam-4823 126 33 are be AUX bam-4823 126 34 very very ADV bam-4823 126 35 well well ADV bam-4823 126 36 resolved resolve VERB bam-4823 126 37 as as ADP bam-4823 126 38 darkly darkly ADV bam-4823 126 39 contrasted contrast VERB bam-4823 126 40 dark dark ADJ bam-4823 126 41 bands band NOUN bam-4823 126 42 that that PRON bam-4823 126 43 sometimes sometimes ADV bam-4823 126 44 are be AUX bam-4823 126 45 bifurcated bifurcate VERB bam-4823 126 46 . . PUNCT bam-4823 127 1 this this DET bam-4823 127 2 appearance appearance NOUN bam-4823 127 3 arises arise VERB bam-4823 127 4 because because SCONJ bam-4823 127 5 the the DET bam-4823 127 6 ryrs ryr NOUN bam-4823 127 7 are be AUX bam-4823 127 8 arranged arrange VERB bam-4823 127 9 as as ADP bam-4823 127 10 two two NUM bam-4823 127 11 side side NOUN bam-4823 127 12 - - PUNCT bam-4823 127 13 by by ADP bam-4823 127 14 - - PUNCT bam-4823 127 15 side side NOUN bam-4823 127 16 rows row NOUN bam-4823 127 17 along along ADP bam-4823 127 18 the the DET bam-4823 127 19 t t NOUN bam-4823 127 20 - - PUNCT bam-4823 127 21 tubule tubule NOUN bam-4823 127 22 . . PUNCT bam-4823 128 1 in in ADP bam-4823 128 2 some some DET bam-4823 128 3 cases case NOUN bam-4823 128 4 , , PUNCT bam-4823 128 5 the the DET bam-4823 128 6 ryrs ryr NOUN bam-4823 128 7 appear appear VERB bam-4823 128 8 to to PART bam-4823 128 9 be be AUX bam-4823 128 10 connected connect VERB bam-4823 128 11 by by ADP bam-4823 128 12 bridges bridge NOUN bam-4823 128 13 of of ADP bam-4823 128 14 density density NOUN bam-4823 128 15 to to ADP bam-4823 128 16 the the DET bam-4823 128 17 ttubule ttubule NOUN bam-4823 128 18 . . PUNCT bam-4823 129 1 however however ADV bam-4823 129 2 , , PUNCT bam-4823 129 3 further further ADJ bam-4823 129 4 study study NOUN bam-4823 129 5 will will AUX bam-4823 129 6 be be AUX bam-4823 129 7 required require VERB bam-4823 129 8 to to PART bam-4823 129 9 discern discern VERB bam-4823 129 10 whether whether SCONJ bam-4823 129 11 these these DET bam-4823 129 12 connections connection NOUN bam-4823 129 13 are be AUX bam-4823 129 14 related relate VERB bam-4823 129 15 to to ADP bam-4823 129 16 the the DET bam-4823 129 17 dihydropyridine dihydropyridine NOUN bam-4823 129 18 receptors receptor NOUN bam-4823 129 19 , , PUNCT bam-4823 129 20 which which PRON bam-4823 129 21 are be AUX bam-4823 129 22 thought think VERB bam-4823 129 23 to to PART bam-4823 129 24 interact interact VERB bam-4823 129 25 and and CCONJ bam-4823 129 26 communicate communicate VERB bam-4823 129 27 directly directly ADV bam-4823 129 28 with with ADP bam-4823 129 29 the the DET bam-4823 129 30 ryrs ryr NOUN bam-4823 129 31 to to PART bam-4823 129 32 carry carry VERB bam-4823 129 33 out out ADP bam-4823 129 34 the the DET bam-4823 129 35 fundamental fundamental ADJ bam-4823 129 36 signal signal NOUN bam-4823 129 37 - - PUNCT bam-4823 129 38 transducing transduce VERB bam-4823 129 39 event event NOUN bam-4823 129 40 of of ADP bam-4823 129 41 excitation excitation NOUN bam-4823 129 42 - - PUNCT bam-4823 129 43 contraction contraction NOUN bam-4823 129 44 . . PUNCT bam-4823 130 1 another another DET bam-4823 130 2 recurring recur VERB bam-4823 130 3 feature feature NOUN bam-4823 130 4 is be AUX bam-4823 130 5 that that SCONJ bam-4823 130 6 the the DET bam-4823 130 7 lumens lumen NOUN bam-4823 130 8 of of ADP bam-4823 130 9 the the DET bam-4823 130 10 t t PROPN bam-4823 130 11 - - PUNCT bam-4823 130 12 tubules tubule NOUN bam-4823 130 13 usually usually ADV bam-4823 130 14 appear appear VERB bam-4823 130 15 to to PART bam-4823 130 16 contain contain VERB bam-4823 130 17 a a DET bam-4823 130 18 substantial substantial ADJ bam-4823 130 19 amount amount NOUN bam-4823 130 20 of of ADP bam-4823 130 21 mass mass NOUN bam-4823 130 22 of of ADP bam-4823 130 23 unknown unknown ADJ bam-4823 130 24 composition composition NOUN bam-4823 130 25 , , PUNCT bam-4823 130 26 but but CCONJ bam-4823 130 27 no no DET bam-4823 130 28 membrane membrane NOUN bam-4823 130 29 associated associate VERB bam-4823 130 30 densities density NOUN bam-4823 130 31 that that PRON bam-4823 130 32 might might AUX bam-4823 130 33 correspond correspond VERB bam-4823 130 34 to to ADP bam-4823 130 35 the the DET bam-4823 130 36 dihydropyridine dihydropyridine NOUN bam-4823 130 37 receptors receptor NOUN bam-4823 130 38 stand stand VERB bam-4823 130 39 out out ADP bam-4823 130 40 from from ADP bam-4823 130 41 the the DET bam-4823 130 42 noise noise NOUN bam-4823 130 43 . . PUNCT bam-4823 131 1 direct direct ADJ bam-4823 131 2 interpretation interpretation NOUN bam-4823 131 3 of of ADP bam-4823 131 4 the the DET bam-4823 131 5 micrographs micrograph NOUN bam-4823 131 6 is be AUX bam-4823 131 7 difficult difficult ADJ bam-4823 131 8 , , PUNCT bam-4823 131 9 not not PART bam-4823 131 10 only only ADV bam-4823 131 11 due due ADJ bam-4823 131 12 to to ADP bam-4823 131 13 the the DET bam-4823 131 14 low low ADJ bam-4823 131 15 contrast contrast NOUN bam-4823 131 16 , , PUNCT bam-4823 131 17 but but CCONJ bam-4823 131 18 also also ADV bam-4823 131 19 because because SCONJ bam-4823 131 20 the the DET bam-4823 131 21 thickness thickness NOUN bam-4823 131 22 of of ADP bam-4823 131 23 the the DET bam-4823 131 24 section section NOUN bam-4823 131 25 ( ( PUNCT bam-4823 131 26 100 100 NUM bam-4823 131 27 - - PUNCT bam-4823 131 28 200 200 NUM bam-4823 131 29 nm nm NOUN bam-4823 131 30 ) ) PUNCT bam-4823 131 31 is be AUX bam-4823 131 32 somewhat somewhat ADV bam-4823 131 33 greater great ADJ bam-4823 131 34 than than SCONJ bam-4823 131 35 is be AUX bam-4823 131 36 usual usual ADJ bam-4823 131 37 for for ADP bam-4823 131 38 conventional conventional ADJ bam-4823 131 39 plastic plastic ADJ bam-4823 131 40 sections section NOUN bam-4823 131 41 , , PUNCT bam-4823 131 42 resulting result VERB bam-4823 131 43 in in ADP bam-4823 131 44 overlap overlap NOUN bam-4823 131 45 of of ADP bam-4823 131 46 structural structural ADJ bam-4823 131 47 features feature NOUN bam-4823 131 48 . . PUNCT bam-4823 132 1 results result NOUN bam-4823 132 2 from from ADP bam-4823 132 3 our our PRON bam-4823 132 4 first first ADJ bam-4823 132 5 attempt attempt NOUN bam-4823 132 6 at at ADP bam-4823 132 7 cryo cryo NOUN bam-4823 132 8 - - PUNCT bam-4823 132 9 et et NOUN bam-4823 132 10 of of ADP bam-4823 132 11 fib fib ADJ bam-4823 132 12 - - PUNCT bam-4823 132 13 milled mill VERB bam-4823 132 14 swimbladder swimbladder NOUN bam-4823 132 15 muscle muscle NOUN bam-4823 132 16 is be AUX bam-4823 132 17 shown show VERB bam-4823 132 18 in in ADP bam-4823 132 19 figure figure NOUN bam-4823 132 20 3 3 NUM bam-4823 132 21 . . NOUN bam-4823 132 22 13 13 NUM bam-4823 132 23 shown show VERB bam-4823 132 24 in in ADP bam-4823 132 25 figure figure NOUN bam-4823 132 26 are be AUX bam-4823 132 27 a a DET bam-4823 132 28 series series NOUN bam-4823 132 29 of of ADP bam-4823 132 30 x x PROPN bam-4823 132 31 - - NOUN bam-4823 132 32 y y NOUN bam-4823 132 33 slices slice NOUN bam-4823 132 34 through through ADP bam-4823 132 35 the the DET bam-4823 132 36 tomogram tomogram NOUN bam-4823 132 37 and and CCONJ bam-4823 132 38 a a DET bam-4823 132 39 three three NUM bam-4823 132 40 - - PUNCT bam-4823 132 41 dimensional dimensional ADJ bam-4823 132 42 representation representation NOUN bam-4823 132 43 that that PRON bam-4823 132 44 shows show VERB bam-4823 132 45 colored colored ADJ bam-4823 132 46 surfaces surface NOUN bam-4823 132 47 for for ADP bam-4823 132 48 the the DET bam-4823 132 49 major major ADJ bam-4823 132 50 recognizable recognizable ADJ bam-4823 132 51 structural structural ADJ bam-4823 132 52 features feature NOUN bam-4823 132 53 ( ( PUNCT bam-4823 132 54 the the DET bam-4823 132 55 sr sr PROPN bam-4823 132 56 and and CCONJ bam-4823 132 57 t t PROPN bam-4823 132 58 - - PUNCT bam-4823 132 59 tubule tubule NOUN bam-4823 132 60 boundaries boundary NOUN bam-4823 132 61 , , PUNCT bam-4823 132 62 ryrs ryr NOUN bam-4823 132 63 , , PUNCT bam-4823 132 64 and and CCONJ bam-4823 132 65 the the DET bam-4823 132 66 luminal luminal ADJ bam-4823 132 67 density density NOUN bam-4823 132 68 in in ADP bam-4823 132 69 the the DET bam-4823 132 70 t t NOUN bam-4823 132 71 - - PUNCT bam-4823 132 72 tubule tubule NOUN bam-4823 132 73 ) ) PUNCT bam-4823 132 74 obtained obtain VERB bam-4823 132 75 by by ADP bam-4823 132 76 manually manually ADV bam-4823 132 77 tracing trace VERB bam-4823 132 78 of of ADP bam-4823 132 79 the the DET bam-4823 132 80 visible visible ADJ bam-4823 132 81 features feature NOUN bam-4823 132 82 in in ADP bam-4823 132 83 each each DET bam-4823 132 84 xyplane xyplane NOUN bam-4823 132 85 slice slice NOUN bam-4823 132 86 of of ADP bam-4823 132 87 the the DET bam-4823 132 88 tomogram tomogram NOUN bam-4823 132 89 ( ( PUNCT bam-4823 132 90 fig fig NOUN bam-4823 132 91 . . PUNCT bam-4823 133 1 3a 3a NUM bam-4823 133 2 , , PUNCT bam-4823 133 3 b b NOUN bam-4823 133 4 , , PUNCT bam-4823 133 5 respectively respectively ADV bam-4823 133 6 ) ) PUNCT bam-4823 133 7 . . PUNCT bam-4823 134 1 figure figure VERB bam-4823 134 2 3c 3c NUM bam-4823 134 3 depicts depict VERB bam-4823 134 4 an an DET bam-4823 134 5 orientation orientation NOUN bam-4823 134 6 of of ADP bam-4823 134 7 a a DET bam-4823 134 8 portion portion NOUN bam-4823 134 9 of of ADP bam-4823 134 10 the the DET bam-4823 134 11 surface surface NOUN bam-4823 134 12 model model NOUN bam-4823 134 13 such such ADJ bam-4823 134 14 that that SCONJ bam-4823 134 15 the the DET bam-4823 134 16 arrangement arrangement NOUN bam-4823 134 17 of of ADP bam-4823 134 18 ryrs ryr NOUN bam-4823 134 19 on on ADP bam-4823 134 20 one one NUM bam-4823 134 21 of of ADP bam-4823 134 22 the the DET bam-4823 134 23 sr sr PROPN bam-4823 134 24 junctional junctional ADJ bam-4823 134 25 faces face NOUN bam-4823 134 26 is be AUX bam-4823 134 27 revealed reveal VERB bam-4823 134 28 . . PUNCT bam-4823 135 1 although although SCONJ bam-4823 135 2 the the DET bam-4823 135 3 individual individual ADJ bam-4823 135 4 ryrs ryr NOUN bam-4823 135 5 are be AUX bam-4823 135 6 not not PART bam-4823 135 7 resolved resolve VERB bam-4823 135 8 from from ADP bam-4823 135 9 one one NUM bam-4823 135 10 another another DET bam-4823 135 11 , , PUNCT bam-4823 135 12 there there PRON bam-4823 135 13 is be VERB bam-4823 135 14 clear clear ADJ bam-4823 135 15 evidence evidence NOUN bam-4823 135 16 for for ADP bam-4823 135 17 two two NUM bam-4823 135 18 rows row NOUN bam-4823 135 19 of of ADP bam-4823 135 20 ryrs ryr NOUN bam-4823 135 21 in in ADP bam-4823 135 22 accordance accordance NOUN bam-4823 135 23 with with ADP bam-4823 135 24 previous previous ADJ bam-4823 135 25 findings finding NOUN bam-4823 135 26 . . PUNCT bam-4823 136 1 the the DET bam-4823 136 2 fib fib ADJ bam-4823 136 3 - - PUNCT bam-4823 136 4 milled mill VERB bam-4823 136 5 section section NOUN bam-4823 136 6 for for ADP bam-4823 136 7 this this DET bam-4823 136 8 experiment experiment NOUN bam-4823 136 9 was be AUX bam-4823 136 10 about about ADV bam-4823 136 11 100 100 NUM bam-4823 136 12 nanometers nanometer NOUN bam-4823 136 13 thick thick ADJ bam-4823 136 14 , , PUNCT bam-4823 136 15 sufficient sufficient ADJ bam-4823 136 16 to to PART bam-4823 136 17 contain contain VERB bam-4823 136 18 about about ADV bam-4823 136 19 three three NUM bam-4823 136 20 ryrs ryr NOUN bam-4823 136 21 from from ADP bam-4823 136 22 each each PRON bam-4823 136 23 of of ADP bam-4823 136 24 the the DET bam-4823 136 25 two two NUM bam-4823 136 26 rows row NOUN bam-4823 136 27 , , PUNCT bam-4823 136 28 and and CCONJ bam-4823 136 29 the the DET bam-4823 136 30 contoured contoured ADJ bam-4823 136 31 model model NOUN bam-4823 136 32 appears appear VERB bam-4823 136 33 to to PART bam-4823 136 34 contain contain VERB bam-4823 136 35 six six NUM bam-4823 136 36 ryrs ryr NOUN bam-4823 136 37 ( ( PUNCT bam-4823 136 38 numbered number VERB bam-4823 136 39 1 1 NUM bam-4823 136 40 - - SYM bam-4823 136 41 6 6 NUM bam-4823 136 42 in in ADP bam-4823 136 43 fig fig NOUN bam-4823 136 44 . . PUNCT bam-4823 137 1 3a 3a NUM bam-4823 137 2 ) ) PUNCT bam-4823 137 3 , , PUNCT bam-4823 137 4 although although SCONJ bam-4823 137 5 those those PRON bam-4823 137 6 at at ADP bam-4823 137 7 the the DET bam-4823 137 8 top top NOUN bam-4823 137 9 of of ADP bam-4823 137 10 the the DET bam-4823 137 11 panel panel NOUN bam-4823 137 12 appear appear VERB bam-4823 137 13 to to PART bam-4823 137 14 be be AUX bam-4823 137 15 truncated truncate VERB bam-4823 137 16 . . PUNCT bam-4823 138 1 the the DET bam-4823 138 2 square square ADV bam-4823 138 3 - - PUNCT bam-4823 138 4 shaped shape VERB bam-4823 138 5 outline outline NOUN bam-4823 138 6 of of ADP bam-4823 138 7 the the DET bam-4823 138 8 ryrs ryr NOUN bam-4823 138 9 , , PUNCT bam-4823 138 10 whose whose DET bam-4823 138 11 overall overall ADJ bam-4823 138 12 structure structure NOUN bam-4823 138 13 is be AUX bam-4823 138 14 known know VERB bam-4823 138 15 from from ADP bam-4823 138 16 previous previous ADJ bam-4823 138 17 work work NOUN bam-4823 138 18 , , PUNCT bam-4823 138 19 is be AUX bam-4823 138 20 barely barely ADV bam-4823 138 21 detectable detectable ADJ bam-4823 138 22 due due ADJ bam-4823 138 23 the the DET bam-4823 138 24 high high ADJ bam-4823 138 25 noise noise NOUN bam-4823 138 26 level level NOUN bam-4823 138 27 . . PUNCT bam-4823 139 1 poor poor ADJ bam-4823 139 2 contrast contrast NOUN bam-4823 139 3 is be AUX bam-4823 139 4 an an DET bam-4823 139 5 inherent inherent ADJ bam-4823 139 6 problem problem NOUN bam-4823 139 7 in in ADP bam-4823 139 8 cryo cryo NOUN bam-4823 139 9 - - PUNCT bam-4823 139 10 et et NOUN bam-4823 139 11 of of ADP bam-4823 139 12 native native ADJ bam-4823 139 13 cells cell NOUN bam-4823 139 14 , , PUNCT bam-4823 139 15 and and CCONJ bam-4823 139 16 is be AUX bam-4823 139 17 further far ADV bam-4823 139 18 compounded compound VERB bam-4823 139 19 , , PUNCT bam-4823 139 20 in in ADP bam-4823 139 21 this this DET bam-4823 139 22 direction direction NOUN bam-4823 139 23 of of ADP bam-4823 139 24 view view NOUN bam-4823 139 25 , , PUNCT bam-4823 139 26 by by ADP bam-4823 139 27 the the DET bam-4823 139 28 missing miss VERB bam-4823 139 29 wedge wedge NOUN bam-4823 139 30 of of ADP bam-4823 139 31 structural structural ADJ bam-4823 139 32 information information NOUN bam-4823 139 33 that that PRON bam-4823 139 34 arises arise VERB bam-4823 139 35 from from ADP bam-4823 139 36 the the DET bam-4823 139 37 limited limited ADJ bam-4823 139 38 tilt tilt NOUN bam-4823 139 39 range range NOUN bam-4823 139 40 in in ADP bam-4823 139 41 the the DET bam-4823 139 42 microscope microscope NOUN bam-4823 139 43 , , PUNCT bam-4823 139 44 in in ADP bam-4823 139 45 this this DET bam-4823 139 46 case case NOUN bam-4823 139 47 ±60 ±60 NOUN bam-4823 139 48 degrees degree NOUN bam-4823 139 49 . . PUNCT bam-4823 140 1 an an DET bam-4823 140 2 additional additional ADJ bam-4823 140 3 unknown unknown NOUN bam-4823 140 4 is be AUX bam-4823 140 5 the the DET bam-4823 140 6 extent extent NOUN bam-4823 140 7 to to PART bam-4823 140 8 which which PRON bam-4823 140 9 additional additional ADJ bam-4823 140 10 proteins protein NOUN bam-4823 140 11 , , PUNCT bam-4823 140 12 for for ADP bam-4823 140 13 instance instance NOUN bam-4823 140 14 subunits subunit NOUN bam-4823 140 15 of of ADP bam-4823 140 16 dhpr dhpr NOUN bam-4823 140 17 , , PUNCT bam-4823 140 18 are be AUX bam-4823 140 19 packed pack VERB bam-4823 140 20 in in ADP bam-4823 140 21 between between ADP bam-4823 140 22 the the DET bam-4823 140 23 ryrs ryr NOUN bam-4823 140 24 which which PRON bam-4823 140 25 at at ADP bam-4823 140 26 low low ADJ bam-4823 140 27 resolution resolution NOUN bam-4823 140 28 could could AUX bam-4823 140 29 confuse confuse VERB bam-4823 140 30 interpretation interpretation NOUN bam-4823 140 31 of of ADP bam-4823 140 32 the the DET bam-4823 140 33 images image NOUN bam-4823 140 34 . . PUNCT bam-4823 141 1 as as ADP bam-4823 141 2 more more ADJ bam-4823 141 3 , , PUNCT bam-4823 141 4 and and CCONJ bam-4823 141 5 better well ADJ bam-4823 141 6 , , PUNCT bam-4823 141 7 tomograms tomogram NOUN bam-4823 141 8 are be AUX bam-4823 141 9 obtained obtain VERB bam-4823 141 10 , , PUNCT bam-4823 141 11 and and CCONJ bam-4823 141 12 subtomogram subtomogram NOUN bam-4823 141 13 averaging averaging NOUN bam-4823 141 14 is be AUX bam-4823 141 15 applied apply VERB bam-4823 141 16 to to ADP bam-4823 141 17 subvolumes subvolume NOUN bam-4823 141 18 containing contain VERB bam-4823 141 19 ryrs ryr NOUN bam-4823 141 20 , , PUNCT bam-4823 141 21 the the DET bam-4823 141 22 structural structural ADJ bam-4823 141 23 organization organization NOUN bam-4823 141 24 of of ADP bam-4823 141 25 the the DET bam-4823 141 26 triad triad PROPN bam-4823 141 27 ’s ’s PART bam-4823 141 28 components component NOUN bam-4823 141 29 should should AUX bam-4823 141 30 become become VERB bam-4823 141 31 much much ADV bam-4823 141 32 better well ADV bam-4823 141 33 resolved resolve VERB bam-4823 141 34 . . PUNCT bam-4823 142 1 outlook outlook NOUN bam-4823 142 2 presently presently ADV bam-4823 142 3 , , PUNCT bam-4823 142 4 the the DET bam-4823 142 5 main main ADJ bam-4823 142 6 drawback drawback NOUN bam-4823 142 7 of of ADP bam-4823 142 8 cryo cryo NOUN bam-4823 142 9 - - PUNCT bam-4823 142 10 fib fib ADJ bam-4823 142 11 preparation preparation NOUN bam-4823 142 12 is be AUX bam-4823 142 13 low low ADJ bam-4823 142 14 throughput throughput NOUN bam-4823 142 15 , , PUNCT bam-4823 142 16 arising arise VERB bam-4823 142 17 at at ADP bam-4823 142 18 several several ADJ bam-4823 142 19 steps step NOUN bam-4823 142 20 in in ADP bam-4823 142 21 the the DET bam-4823 142 22 process process NOUN bam-4823 142 23 . . PUNCT bam-4823 143 1 even even ADV bam-4823 143 2 when when SCONJ bam-4823 143 3 all all PRON bam-4823 143 4 of of ADP bam-4823 143 5 the the DET bam-4823 143 6 steps step NOUN bam-4823 143 7 described describe VERB bam-4823 143 8 above above ADV bam-4823 143 9 are be AUX bam-4823 143 10 successful successful ADJ bam-4823 143 11 , , PUNCT bam-4823 143 12 only only ADV bam-4823 143 13 about about ADV bam-4823 143 14 eight eight NUM bam-4823 143 15 useful useful ADJ bam-4823 143 16 10 10 NUM bam-4823 143 17 µm µm NOUN bam-4823 143 18 x x SYM bam-4823 143 19 20 20 NUM bam-4823 143 20 µm µm ADP bam-4823 143 21 lamellae lamellae NOUN bam-4823 143 22 are be AUX bam-4823 143 23 produced produce VERB bam-4823 143 24 in in ADP bam-4823 143 25 each each DET bam-4823 143 26 specimen speciman NOUN bam-4823 143 27 , , PUNCT bam-4823 143 28 yielding yield VERB bam-4823 143 29 a a DET bam-4823 143 30 total total ADJ bam-4823 143 31 area area NOUN bam-4823 143 32 of of ADP bam-4823 143 33 about about ADV bam-4823 143 34 1600 1600 NUM bam-4823 143 35 µm µm SYM bam-4823 143 36 2 2 NUM bam-4823 143 37 suitable suitable ADJ bam-4823 143 38 for for ADP bam-4823 143 39 tomographic tomographic ADJ bam-4823 143 40 data datum NOUN bam-4823 143 41 collection collection NOUN bam-4823 143 42 . . PUNCT bam-4823 144 1 this this DET bam-4823 144 2 area area NOUN bam-4823 144 3 corresponds correspond VERB bam-4823 144 4 to to ADP bam-4823 144 5 only only ADV bam-4823 144 6 about about ADP bam-4823 144 7 fig fig NOUN bam-4823 144 8 3 3 NUM bam-4823 144 9 . . PUNCT bam-4823 145 1 cryo cryo NOUN bam-4823 145 2 - - PUNCT bam-4823 145 3 et et PROPN bam-4823 145 4 of of ADP bam-4823 145 5 fib fib ADJ bam-4823 145 6 - - PUNCT bam-4823 145 7 milled mill VERB bam-4823 145 8 toadfish toadfish ADJ bam-4823 145 9 swimbladder swimbladder NOUN bam-4823 145 10 muscle muscle NOUN bam-4823 145 11 . . PUNCT bam-4823 146 1 13 13 NUM bam-4823 146 2 a. a. NOUN bam-4823 146 3 x x PROPN bam-4823 146 4 - - PUNCT bam-4823 146 5 y y PROPN bam-4823 146 6 slices slice NOUN bam-4823 146 7 through through ADP bam-4823 146 8 the the DET bam-4823 146 9 tomogram tomogram NOUN bam-4823 146 10 determined determine VERB bam-4823 146 11 for for ADP bam-4823 146 12 the the DET bam-4823 146 13 triad triad NOUN bam-4823 146 14 shown show VERB bam-4823 146 15 in in ADP bam-4823 146 16 fig fig NOUN bam-4823 146 17 . . PUNCT bam-4823 147 1 2b 2b NUM bam-4823 147 2 ( ( PUNCT bam-4823 147 3 section section NOUN bam-4823 147 4 thickness thickness NOUN bam-4823 147 5 ̴100 ̴100 PROPN bam-4823 147 6 nm nm NOUN bam-4823 147 7 ) ) PUNCT bam-4823 147 8 . . PUNCT bam-4823 148 1 slices slice NOUN bam-4823 148 2 are be AUX bam-4823 148 3 1 1 NUM bam-4823 148 4 nm nm ADV bam-4823 148 5 thick thick ADJ bam-4823 148 6 , , PUNCT bam-4823 148 7 spaced space VERB bam-4823 148 8 at at ADP bam-4823 148 9 10 10 NUM bam-4823 148 10 nm nm NOUN bam-4823 148 11 intervals interval NOUN bam-4823 148 12 along along ADP bam-4823 148 13 z. z. PROPN bam-4823 148 14 b. b. PROPN bam-4823 148 15 manually manually ADV bam-4823 148 16 segmented segment VERB bam-4823 148 17 model model NOUN bam-4823 148 18 in in ADP bam-4823 148 19 the the DET bam-4823 148 20 region region NOUN bam-4823 148 21 of of ADP bam-4823 148 22 the the DET bam-4823 148 23 triad triad ADJ bam-4823 148 24 junction junction NOUN bam-4823 148 25 . . PUNCT bam-4823 149 1 green green ADJ bam-4823 149 2 , , PUNCT bam-4823 149 3 t t NOUN bam-4823 149 4 - - PUNCT bam-4823 149 5 tubule tubule NOUN bam-4823 149 6 ; ; PUNCT bam-4823 149 7 blue blue ADJ bam-4823 149 8 , , PUNCT bam-4823 149 9 density density NOUN bam-4823 149 10 containing contain VERB bam-4823 149 11 ryanodine ryanodine NOUN bam-4823 149 12 receptors receptor NOUN bam-4823 149 13 ; ; PUNCT bam-4823 149 14 yellow yellow ADJ bam-4823 149 15 , , PUNCT bam-4823 149 16 sr sr PROPN bam-4823 149 17 membrane membrane NOUN bam-4823 149 18 ; ; PUNCT bam-4823 149 19 purple purple ADJ bam-4823 149 20 , , PUNCT bam-4823 149 21 unidentified unidentified ADJ bam-4823 149 22 mass mass NOUN bam-4823 149 23 density density NOUN bam-4823 149 24 in in ADP bam-4823 149 25 lumen luman NOUN bam-4823 149 26 of of ADP bam-4823 149 27 t t NOUN bam-4823 149 28 - - PUNCT bam-4823 149 29 tubule tubule NOUN bam-4823 149 30 . . PUNCT bam-4823 150 1 c. c. NOUN bam-4823 150 2 view view NOUN bam-4823 150 3 onto onto ADP bam-4823 150 4 ryr ryr NOUN bam-4823 150 5 - - PUNCT bam-4823 150 6 bearing bear VERB bam-4823 150 7 surface surface NOUN bam-4823 150 8 of of ADP bam-4823 150 9 sr sr PROPN bam-4823 150 10 ( ( PUNCT bam-4823 150 11 from from ADP bam-4823 150 12 upper upper PROPN bam-4823 150 13 sr sr PROPN bam-4823 150 14 in in ADP bam-4823 150 15 b b PROPN bam-4823 150 16 ) ) PUNCT bam-4823 150 17 showing show VERB bam-4823 150 18 4 4 NUM bam-4823 150 19 - - SYM bam-4823 150 20 6 6 NUM bam-4823 150 21 ryrs ryr NOUN bam-4823 150 22 ( ( PUNCT bam-4823 150 23 numbered number VERB bam-4823 150 24 ) ) PUNCT bam-4823 150 25 , , PUNCT bam-4823 150 26 which which PRON bam-4823 150 27 are be AUX bam-4823 150 28 not not PART bam-4823 150 29 clearly clearly ADV bam-4823 150 30 resolved resolve VERB bam-4823 150 31 from from ADP bam-4823 150 32 one one NUM bam-4823 150 33 another another DET bam-4823 150 34 . . PUNCT bam-4823 151 1 parts part NOUN bam-4823 151 2 of of ADP bam-4823 151 3 a a DET bam-4823 151 4 adapted adapt VERB bam-4823 151 5 from from ADP bam-4823 151 6 reference reference NOUN bam-4823 151 7 . . PUNCT bam-4823 152 1 13 13 NUM bam-4823 152 2 skeletal skeletal ADJ bam-4823 152 3 muscle muscle NOUN bam-4823 152 4 triad triad VERB bam-4823 152 5 by by ADP bam-4823 152 6 fib fib NOUN bam-4823 152 7 milling milling NOUN bam-4823 152 8 and and CCONJ bam-4823 152 9 cryo cryo NOUN bam-4823 152 10 - - PUNCT bam-4823 152 11 et et PROPN bam-4823 152 12 eur eur PROPN bam-4823 152 13 j j PROPN bam-4823 152 14 transl transl PROPN bam-4823 152 15 myol myol PROPN bam-4823 152 16 basic basic ADJ bam-4823 152 17 appl appl NOUN bam-4823 152 18 myol myol NOUN bam-4823 152 19 2015 2015 NUM bam-4823 152 20 ; ; PUNCT bam-4823 152 21 25 25 NUM bam-4823 152 22 ( ( PUNCT bam-4823 152 23 1 1 NUM bam-4823 152 24 ): ): PUNCT bam-4823 152 25 49 49 NUM bam-4823 152 26 - - SYM bam-4823 152 27 56 56 NUM bam-4823 152 28 55 55 NUM bam-4823 152 29 20 20 NUM bam-4823 152 30 % % NOUN bam-4823 152 31 of of ADP bam-4823 152 32 the the DET bam-4823 152 33 area area NOUN bam-4823 152 34 of of ADP bam-4823 152 35 a a DET bam-4823 152 36 single single ADJ bam-4823 152 37 grid grid NOUN bam-4823 152 38 square square NOUN bam-4823 152 39 of of ADP bam-4823 152 40 a a DET bam-4823 152 41 200 200 NUM bam-4823 152 42 - - PUNCT bam-4823 152 43 mesh mesh NOUN bam-4823 152 44 grid grid NOUN bam-4823 152 45 which which PRON bam-4823 152 46 contains contain VERB bam-4823 152 47 about about ADP bam-4823 152 48 50 50 NUM bam-4823 152 49 grid grid NOUN bam-4823 152 50 squares square NOUN bam-4823 152 51 suitable suitable ADJ bam-4823 152 52 for for ADP bam-4823 152 53 full full ADJ bam-4823 152 54 - - PUNCT bam-4823 152 55 tilt tilt NOUN bam-4823 152 56 - - PUNCT bam-4823 152 57 range range NOUN bam-4823 152 58 imaging imaging NOUN bam-4823 152 59 . . PUNCT bam-4823 153 1 clearly clearly ADV bam-4823 153 2 , , PUNCT bam-4823 153 3 it it PRON bam-4823 153 4 is be AUX bam-4823 153 5 essential essential ADJ bam-4823 153 6 that that SCONJ bam-4823 153 7 most most ADV bam-4823 153 8 milled mill VERB bam-4823 153 9 areas area NOUN bam-4823 153 10 contain contain VERB bam-4823 153 11 the the DET bam-4823 153 12 structure(s structure(s NOUN bam-4823 153 13 ) ) PUNCT bam-4823 153 14 of of ADP bam-4823 153 15 interest interest NOUN bam-4823 153 16 , , PUNCT bam-4823 153 17 which which PRON bam-4823 153 18 is be AUX bam-4823 153 19 indeed indeed ADV bam-4823 153 20 the the DET bam-4823 153 21 case case NOUN bam-4823 153 22 for for ADP bam-4823 153 23 muscle muscle NOUN bam-4823 153 24 tissue tissue NOUN bam-4823 153 25 . . PUNCT bam-4823 154 1 however however ADV bam-4823 154 2 , , PUNCT bam-4823 154 3 for for ADP bam-4823 154 4 some some DET bam-4823 154 5 specimens specimen NOUN bam-4823 154 6 this this PRON bam-4823 154 7 is be AUX bam-4823 154 8 not not PART bam-4823 154 9 achievable achievable ADJ bam-4823 154 10 because because SCONJ bam-4823 154 11 structures structure NOUN bam-4823 154 12 at at ADP bam-4823 154 13 the the DET bam-4823 154 14 surface surface NOUN bam-4823 154 15 of of ADP bam-4823 154 16 a a DET bam-4823 154 17 vitreously vitreously ADV bam-4823 154 18 frozen frozen ADJ bam-4823 154 19 sample sample NOUN bam-4823 154 20 are be AUX bam-4823 154 21 very very ADV bam-4823 154 22 poorly poorly ADV bam-4823 154 23 imaged image VERB bam-4823 154 24 by by ADP bam-4823 154 25 the the DET bam-4823 154 26 electron electron NOUN bam-4823 154 27 or or CCONJ bam-4823 154 28 ion ion NOUN bam-4823 154 29 beam beam NOUN bam-4823 154 30 , , PUNCT bam-4823 154 31 and and CCONJ bam-4823 154 32 structures structure NOUN bam-4823 154 33 in in ADP bam-4823 154 34 the the DET bam-4823 154 35 depth depth NOUN bam-4823 154 36 of of ADP bam-4823 154 37 a a DET bam-4823 154 38 sample sample NOUN bam-4823 154 39 can can AUX bam-4823 154 40 not not PART bam-4823 154 41 be be AUX bam-4823 154 42 directly directly ADV bam-4823 154 43 located locate VERB bam-4823 154 44 at at ADV bam-4823 154 45 all all ADV bam-4823 154 46 . . PUNCT bam-4823 155 1 in in ADP bam-4823 155 2 our our PRON bam-4823 155 3 case case NOUN bam-4823 155 4 , , PUNCT bam-4823 155 5 it it PRON bam-4823 155 6 is be AUX bam-4823 155 7 feasible feasible ADJ bam-4823 155 8 to to PART bam-4823 155 9 obtain obtain VERB bam-4823 155 10 10 10 NUM bam-4823 155 11 or or CCONJ bam-4823 155 12 more more ADJ bam-4823 155 13 tomograms tomogram NOUN bam-4823 155 14 per per ADP bam-4823 155 15 successful successful ADJ bam-4823 155 16 fibmilling fibmille VERB bam-4823 155 17 session session NOUN bam-4823 155 18 , , PUNCT bam-4823 155 19 with with ADP bam-4823 155 20 each each DET bam-4823 155 21 tomogram tomogram NOUN bam-4823 155 22 containing contain VERB bam-4823 155 23 5 5 NUM bam-4823 155 24 - - SYM bam-4823 155 25 6 6 NUM bam-4823 155 26 triad triad ADJ bam-4823 155 27 junctions junction NOUN bam-4823 155 28 . . PUNCT bam-4823 156 1 in in ADP bam-4823 156 2 contrast contrast NOUN bam-4823 156 3 to to ADP bam-4823 156 4 this this DET bam-4823 156 5 situation situation NOUN bam-4823 156 6 , , PUNCT bam-4823 156 7 cryo cryo NOUN bam-4823 156 8 - - PUNCT bam-4823 156 9 et et NOUN bam-4823 156 10 of of ADP bam-4823 156 11 bacteria bacteria NOUN bam-4823 156 12 and and CCONJ bam-4823 156 13 small small ADJ bam-4823 156 14 cells cell NOUN bam-4823 156 15 that that PRON bam-4823 156 16 can can AUX bam-4823 156 17 be be AUX bam-4823 156 18 deposited deposit VERB bam-4823 156 19 over over ADP bam-4823 156 20 the the DET bam-4823 156 21 entire entire ADJ bam-4823 156 22 surface surface NOUN bam-4823 156 23 of of ADP bam-4823 156 24 a a DET bam-4823 156 25 grid grid NOUN bam-4823 156 26 , , PUNCT bam-4823 156 27 and and CCONJ bam-4823 156 28 which which PRON bam-4823 156 29 do do AUX bam-4823 156 30 not not PART bam-4823 156 31 require require VERB bam-4823 156 32 fib fib ADJ bam-4823 156 33 milling milling NOUN bam-4823 156 34 , , PUNCT bam-4823 156 35 can can AUX bam-4823 156 36 yield yield VERB bam-4823 156 37 tens ten NOUN bam-4823 156 38 to to PART bam-4823 156 39 hundreds hundred NOUN bam-4823 156 40 of of ADP bam-4823 156 41 tomograms tomogram NOUN bam-4823 156 42 from from ADP bam-4823 156 43 a a DET bam-4823 156 44 single single ADJ bam-4823 156 45 grid grid NOUN bam-4823 156 46 , , PUNCT bam-4823 156 47 by by ADP bam-4823 156 48 means mean NOUN bam-4823 156 49 of of ADP bam-4823 156 50 automated automate VERB bam-4823 156 51 microscopy microscopy NOUN bam-4823 156 52 . . PUNCT bam-4823 157 1 25,26 25,26 NUM bam-4823 157 2 much much ADJ bam-4823 157 3 effort effort NOUN bam-4823 157 4 will will AUX bam-4823 157 5 be be AUX bam-4823 157 6 required require VERB bam-4823 157 7 to to PART bam-4823 157 8 improve improve VERB bam-4823 157 9 the the DET bam-4823 157 10 efficiency efficiency NOUN bam-4823 157 11 of of ADP bam-4823 157 12 cryo cryo NOUN bam-4823 157 13 - - PUNCT bam-4823 157 14 fib fib NOUN bam-4823 157 15 milling milling NOUN bam-4823 157 16 to to ADP bam-4823 157 17 the the DET bam-4823 157 18 point point NOUN bam-4823 157 19 where where SCONJ bam-4823 157 20 it it PRON bam-4823 157 21 is be AUX bam-4823 157 22 practiced practice VERB bam-4823 157 23 routinely routinely ADV bam-4823 157 24 . . PUNCT bam-4823 158 1 the the DET bam-4823 158 2 problem problem NOUN bam-4823 158 3 of of ADP bam-4823 158 4 identifying identify VERB bam-4823 158 5 regions region NOUN bam-4823 158 6 of of ADP bam-4823 158 7 the the DET bam-4823 158 8 frozen frozen ADJ bam-4823 158 9 specimen speciman NOUN bam-4823 158 10 that that PRON bam-4823 158 11 contain contain VERB bam-4823 158 12 the the DET bam-4823 158 13 regions region NOUN bam-4823 158 14 of of ADP bam-4823 158 15 interest interest NOUN bam-4823 158 16 is be AUX bam-4823 158 17 being be AUX bam-4823 158 18 addressed address VERB bam-4823 158 19 by by ADP bam-4823 158 20 correlative correlative ADJ bam-4823 158 21 light light ADJ bam-4823 158 22 microscopy microscopy NOUN bam-4823 158 23 . . PUNCT bam-4823 159 1 the the DET bam-4823 159 2 rationale rationale NOUN bam-4823 159 3 is be AUX bam-4823 159 4 to to PART bam-4823 159 5 label label VERB bam-4823 159 6 the the DET bam-4823 159 7 structure structure NOUN bam-4823 159 8 of of ADP bam-4823 159 9 interest interest NOUN bam-4823 159 10 with with ADP bam-4823 159 11 a a DET bam-4823 159 12 fluorescent fluorescent ADJ bam-4823 159 13 tag tag NOUN bam-4823 159 14 , , PUNCT bam-4823 159 15 image image VERB bam-4823 159 16 it it PRON bam-4823 159 17 in in ADP bam-4823 159 18 3 3 NUM bam-4823 159 19 - - SYM bam-4823 159 20 d d NOUN bam-4823 159 21 by by ADP bam-4823 159 22 confocal confocal ADJ bam-4823 159 23 fluorescence fluorescence NOUN bam-4823 159 24 microscopy microscopy NOUN bam-4823 159 25 in in ADP bam-4823 159 26 the the DET bam-4823 159 27 frozen frozen ADJ bam-4823 159 28 state state NOUN bam-4823 159 29 , , PUNCT bam-4823 159 30 and and CCONJ bam-4823 159 31 then then ADV bam-4823 159 32 translate translate VERB bam-4823 159 33 the the DET bam-4823 159 34 coordinates coordinate NOUN bam-4823 159 35 to to ADP bam-4823 159 36 the the DET bam-4823 159 37 fib fib NOUN bam-4823 159 38 - - PUNCT bam-4823 159 39 sem sem NOUN bam-4823 159 40 for for ADP bam-4823 159 41 milling milling NOUN bam-4823 159 42 . . PUNCT bam-4823 160 1 21,27 21,27 NUM bam-4823 160 2 it it PRON bam-4823 160 3 may may AUX bam-4823 160 4 also also ADV bam-4823 160 5 be be AUX bam-4823 160 6 possible possible ADJ bam-4823 160 7 to to PART bam-4823 160 8 immediately immediately ADV bam-4823 160 9 check check VERB bam-4823 160 10 finished finish VERB bam-4823 160 11 lamellae lamellae NOUN bam-4823 160 12 for for ADP bam-4823 160 13 presence presence NOUN bam-4823 160 14 of of ADP bam-4823 160 15 a a DET bam-4823 160 16 desired desire VERB bam-4823 160 17 structure structure NOUN bam-4823 160 18 by by ADP bam-4823 160 19 use use NOUN bam-4823 160 20 of of ADP bam-4823 160 21 a a DET bam-4823 160 22 scanning scanning NOUN bam-4823 160 23 - - PUNCT bam-4823 160 24 transmission transmission NOUN bam-4823 160 25 detector detector NOUN bam-4823 160 26 in in ADP bam-4823 160 27 the the DET bam-4823 160 28 fib fib ADJ bam-4823 160 29 - - PUNCT bam-4823 160 30 sem sem NOUN bam-4823 160 31 instrument instrument NOUN bam-4823 160 32 . . PUNCT bam-4823 161 1 29 29 NUM bam-4823 162 1 with with ADP bam-4823 162 2 regard regard NOUN bam-4823 162 3 to to ADP bam-4823 162 4 improving improve VERB bam-4823 162 5 the the DET bam-4823 162 6 efficiency efficiency NOUN bam-4823 162 7 of of ADP bam-4823 162 8 the the DET bam-4823 162 9 fib fib NOUN bam-4823 162 10 milling milling NOUN bam-4823 162 11 itself itself PRON bam-4823 162 12 , , PUNCT bam-4823 162 13 the the DET bam-4823 162 14 specimen speciman NOUN bam-4823 162 15 configuration configuration NOUN bam-4823 162 16 and and CCONJ bam-4823 162 17 pre pre ADJ bam-4823 162 18 - - ADJ bam-4823 162 19 trimming trim VERB bam-4823 162 20 geometry geometry NOUN bam-4823 162 21 that that PRON bam-4823 162 22 we we PRON bam-4823 162 23 use use VERB bam-4823 162 24 lends lend VERB bam-4823 162 25 itself itself PRON bam-4823 162 26 to to ADP bam-4823 162 27 automated automate VERB bam-4823 162 28 production production NOUN bam-4823 162 29 of of ADP bam-4823 162 30 the the DET bam-4823 162 31 lamellae lamellae NOUN bam-4823 162 32 , , PUNCT bam-4823 162 33 once once SCONJ bam-4823 162 34 the the DET bam-4823 162 35 sample sample NOUN bam-4823 162 36 is be AUX bam-4823 162 37 aligned align VERB bam-4823 162 38 in in ADP bam-4823 162 39 the the DET bam-4823 162 40 fib fib ADJ bam-4823 162 41 - - PUNCT bam-4823 162 42 sem sem NOUN bam-4823 162 43 instrument instrument NOUN bam-4823 162 44 . . PUNCT bam-4823 163 1 despite despite SCONJ bam-4823 163 2 the the DET bam-4823 163 3 limitations limitation NOUN bam-4823 163 4 of of ADP bam-4823 163 5 fib fib ADJ bam-4823 163 6 - - PUNCT bam-4823 163 7 milling mill VERB bam-4823 163 8 applications application NOUN bam-4823 163 9 to to ADP bam-4823 163 10 tissues tissue NOUN bam-4823 163 11 and and CCONJ bam-4823 163 12 cells cell NOUN bam-4823 163 13 , , PUNCT bam-4823 163 14 current current ADJ bam-4823 163 15 technology technology NOUN bam-4823 163 16 should should AUX bam-4823 163 17 allow allow VERB bam-4823 163 18 progress progress NOUN bam-4823 163 19 to to PART bam-4823 163 20 made made VERB bam-4823 163 21 on on ADP bam-4823 163 22 favorable favorable ADJ bam-4823 163 23 specimens specimen NOUN bam-4823 163 24 , , PUNCT bam-4823 163 25 such such ADJ bam-4823 163 26 as as ADP bam-4823 163 27 the the DET bam-4823 163 28 triad triad ADJ bam-4823 163 29 junction junction NOUN bam-4823 163 30 discussed discuss VERB bam-4823 163 31 here here ADV bam-4823 163 32 . . PUNCT bam-4823 164 1 already already ADV bam-4823 164 2 , , PUNCT bam-4823 164 3 it it PRON bam-4823 164 4 should should AUX bam-4823 164 5 be be AUX bam-4823 164 6 feasible feasible ADJ bam-4823 164 7 to to PART bam-4823 164 8 acquire acquire VERB bam-4823 164 9 , , PUNCT bam-4823 164 10 over over ADP bam-4823 164 11 a a DET bam-4823 164 12 period period NOUN bam-4823 164 13 of of ADP bam-4823 164 14 months month NOUN bam-4823 164 15 , , PUNCT bam-4823 164 16 100 100 NUM bam-4823 164 17 or or CCONJ bam-4823 164 18 more more ADJ bam-4823 164 19 triad triad ADJ bam-4823 164 20 - - PUNCT bam-4823 164 21 junction junction NOUN bam-4823 164 22 sub sub NOUN bam-4823 164 23 - - NOUN bam-4823 164 24 tomograms tomogram NOUN bam-4823 164 25 from from ADP bam-4823 164 26 wellpreserved wellpreserve VERB bam-4823 164 27 , , PUNCT bam-4823 164 28 thin thin ADJ bam-4823 164 29 ( ( PUNCT bam-4823 164 30 100 100 NUM bam-4823 164 31 - - PUNCT bam-4823 164 32 200 200 NUM bam-4823 164 33 nm nm NOUN bam-4823 164 34 ) ) PUNCT bam-4823 164 35 lamellae lamellae NOUN bam-4823 164 36 . . PUNCT bam-4823 165 1 this this DET bam-4823 165 2 data datum NOUN bam-4823 165 3 should should AUX bam-4823 165 4 be be AUX bam-4823 165 5 sufficient sufficient ADJ bam-4823 165 6 to to PART bam-4823 165 7 begin begin VERB bam-4823 165 8 realizing realize VERB bam-4823 165 9 the the DET bam-4823 165 10 full full ADJ bam-4823 165 11 and and CCONJ bam-4823 165 12 evolving evolve VERB bam-4823 165 13 potential potential NOUN bam-4823 165 14 of of ADP bam-4823 165 15 cryo cryo NOUN bam-4823 165 16 - - PUNCT bam-4823 165 17 electron electron NOUN bam-4823 165 18 tomography tomography NOUN bam-4823 165 19 , , PUNCT bam-4823 165 20 as as SCONJ bam-4823 165 21 exemplified exemplify VERB bam-4823 165 22 by by ADP bam-4823 165 23 recent recent ADJ bam-4823 165 24 studies study NOUN bam-4823 165 25 of of ADP bam-4823 165 26 thin thin ADJ bam-4823 165 27 ( ( PUNCT bam-4823 165 28 i.e. i.e. X bam-4823 165 29 not not PART bam-4823 165 30 requiring require VERB bam-4823 165 31 fib fib NOUN bam-4823 165 32 ) ) PUNCT bam-4823 165 33 , , PUNCT bam-4823 165 34 plunge plunge NOUN bam-4823 165 35 - - PUNCT bam-4823 165 36 frozen frozen ADJ bam-4823 165 37 single single ADJ bam-4823 165 38 cells cell NOUN bam-4823 165 39 . . PUNCT bam-4823 166 1 5,6,28 5,6,28 NUM bam-4823 166 2 currently currently ADV bam-4823 166 3 , , PUNCT bam-4823 166 4 a a DET bam-4823 166 5 limitation limitation NOUN bam-4823 166 6 of of ADP bam-4823 166 7 cryo cryo NOUN bam-4823 166 8 - - PUNCT bam-4823 166 9 et et NOUN bam-4823 166 10 is be AUX bam-4823 166 11 that that SCONJ bam-4823 166 12 generally generally ADV bam-4823 166 13 applicable applicable ADJ bam-4823 166 14 strategies strategy NOUN bam-4823 166 15 for for ADP bam-4823 166 16 labeling labeling NOUN bam-4823 166 17 proteins protein NOUN bam-4823 166 18 of of ADP bam-4823 166 19 interest interest NOUN bam-4823 166 20 have have AUX bam-4823 166 21 not not PART bam-4823 166 22 been be AUX bam-4823 166 23 perfected perfect VERB bam-4823 166 24 6 6 NUM bam-4823 166 25 . . PUNCT bam-4823 167 1 however however ADV bam-4823 167 2 , , PUNCT bam-4823 167 3 by by ADP bam-4823 167 4 means mean NOUN bam-4823 167 5 of of ADP bam-4823 167 6 sub sub ADJ bam-4823 167 7 - - ADJ bam-4823 167 8 tomogram tomogram ADJ bam-4823 167 9 averaging averaging NOUN bam-4823 167 10 , , PUNCT bam-4823 167 11 large large ADJ bam-4823 167 12 macromolecular macromolecular NOUN bam-4823 167 13 complexes complex NOUN bam-4823 167 14 , , PUNCT bam-4823 167 15 such such ADJ bam-4823 167 16 as as ADP bam-4823 167 17 ryanodine ryanodine NOUN bam-4823 167 18 receptors receptor NOUN bam-4823 167 19 that that PRON bam-4823 167 20 are be AUX bam-4823 167 21 identifiable identifiable ADJ bam-4823 167 22 from from ADP bam-4823 167 23 their their PRON bam-4823 167 24 known know VERB bam-4823 167 25 structures structure NOUN bam-4823 167 26 , , PUNCT bam-4823 167 27 can can AUX bam-4823 167 28 be be AUX bam-4823 167 29 extracted extract VERB bam-4823 167 30 from from ADP bam-4823 167 31 tomograms tomogram NOUN bam-4823 167 32 and and CCONJ bam-4823 167 33 combined combine VERB bam-4823 167 34 to to PART bam-4823 167 35 produce produce VERB bam-4823 167 36 3d 3d NUM bam-4823 167 37 reconstructions reconstruction NOUN bam-4823 167 38 having have VERB bam-4823 167 39 resolutions resolution NOUN bam-4823 167 40 of of ADP bam-4823 167 41 2 2 NUM bam-4823 167 42 - - SYM bam-4823 167 43 4 4 NUM bam-4823 167 44 nm nm NOUN bam-4823 167 45 , , PUNCT bam-4823 167 46 thereby thereby ADV bam-4823 167 47 revealing reveal VERB bam-4823 167 48 their their PRON bam-4823 167 49 structural structural ADJ bam-4823 167 50 state state NOUN bam-4823 167 51 in in ADP bam-4823 167 52 an an PRON bam-4823 167 53 in in ADP bam-4823 167 54 situ situ ADJ bam-4823 167 55 environment environment NOUN bam-4823 167 56 . . PUNCT bam-4823 168 1 when when SCONJ bam-4823 168 2 applied apply VERB bam-4823 168 3 to to ADP bam-4823 168 4 ryrs ryr NOUN bam-4823 168 5 in in ADP bam-4823 168 6 triads triad NOUN bam-4823 168 7 , , PUNCT bam-4823 168 8 structural structural ADJ bam-4823 168 9 information information NOUN bam-4823 168 10 on on ADP bam-4823 168 11 proteins protein NOUN bam-4823 168 12 that that PRON bam-4823 168 13 interact interact VERB bam-4823 168 14 with with ADP bam-4823 168 15 ryrs ryr NOUN bam-4823 168 16 , , PUNCT bam-4823 168 17 such such ADJ bam-4823 168 18 as as ADP bam-4823 168 19 calmodulin calmodulin NOUN bam-4823 168 20 , , PUNCT bam-4823 168 21 the the DET bam-4823 168 22 dihydropyridine dihydropyridine NOUN bam-4823 168 23 receptor receptor NOUN bam-4823 168 24 , , PUNCT bam-4823 168 25 and and CCONJ bam-4823 168 26 triadin triadin NOUN bam-4823 168 27 / / SYM bam-4823 168 28 calsequestrin calsequestrin PROPN bam-4823 168 29 should should AUX bam-4823 168 30 be be AUX bam-4823 168 31 resolved resolve VERB bam-4823 168 32 . . PUNCT bam-4823 169 1 this this PRON bam-4823 169 2 would would AUX bam-4823 169 3 certainly certainly ADV bam-4823 169 4 advance advance VERB bam-4823 169 5 current current ADJ bam-4823 169 6 understanding understanding NOUN bam-4823 169 7 of of ADP bam-4823 169 8 structure structure NOUN bam-4823 169 9 - - PUNCT bam-4823 169 10 function function NOUN bam-4823 169 11 relationships relationship NOUN bam-4823 169 12 for for ADP bam-4823 169 13 the the DET bam-4823 169 14 triad triad ADJ bam-4823 169 15 junction junction NOUN bam-4823 169 16 . . PUNCT bam-4823 170 1 acknowledgement acknowledgement NOUN bam-4823 170 2 the the DET bam-4823 170 3 authors author NOUN bam-4823 170 4 thank thank VERB bam-4823 170 5 dr dr PROPN bam-4823 170 6 . . PROPN bam-4823 170 7 hongli hongli PROPN bam-4823 170 8 hu hu PROPN bam-4823 171 1 for for ADP bam-4823 171 2 help help NOUN bam-4823 171 3 with with ADP bam-4823 171 4 preparing prepare VERB bam-4823 171 5 figure figure NOUN bam-4823 171 6 3 3 NUM bam-4823 171 7 . . PUNCT bam-4823 172 1 this this DET bam-4823 172 2 work work NOUN bam-4823 172 3 was be AUX bam-4823 172 4 supported support VERB bam-4823 172 5 by by ADP bam-4823 172 6 nih nih PROPN bam-4823 172 7 grants grant NOUN bam-4823 172 8 r01ar40615 r01ar40615 NOUN bam-4823 172 9 ( ( PUNCT bam-4823 172 10 to to ADP bam-4823 172 11 t. t. PROPN bam-4823 172 12 wagenknecht wagenknecht PROPN bam-4823 172 13 ) ) PUNCT bam-4823 172 14 and and CCONJ bam-4823 172 15 r01gm097010 r01gm097010 PROPN bam-4823 172 16 ( ( PUNCT bam-4823 172 17 to to PART bam-4823 172 18 m. m. PROPN bam-4823 172 19 marko marko PROPN bam-4823 172 20 ) ) PUNCT bam-4823 172 21 . . PUNCT bam-4823 173 1 microscopy microscopy NOUN bam-4823 173 2 infrastructure infrastructure NOUN bam-4823 173 3 was be AUX bam-4823 173 4 provided provide VERB bam-4823 173 5 by by ADP bam-4823 173 6 the the DET bam-4823 173 7 wadsworth wadsworth NOUN bam-4823 173 8 center center NOUN bam-4823 173 9 ’s ’s PART bam-4823 173 10 3d 3d PROPN bam-4823 173 11 - - PUNCT bam-4823 173 12 em em PRON bam-4823 173 13 facility facility NOUN bam-4823 173 14 . . PUNCT bam-4823 174 1 contributions contribution NOUN bam-4823 174 2 of of ADP bam-4823 174 3 authors author NOUN bam-4823 174 4 this this DET bam-4823 174 5 review review NOUN bam-4823 174 6 was be AUX bam-4823 174 7 written write VERB bam-4823 174 8 jointly jointly ADV bam-4823 174 9 by by ADP bam-4823 174 10 the the DET bam-4823 174 11 three three NUM bam-4823 174 12 authors author NOUN bam-4823 174 13 . . PUNCT bam-4823 175 1 corresponding correspond VERB bam-4823 175 2 author author NOUN bam-4823 175 3 t. t. PROPN bam-4823 175 4 wagenknecht wagenknecht PROPN bam-4823 175 5 , , PUNCT bam-4823 175 6 biggs biggs PROPN bam-4823 175 7 laboratory laboratory PROPN bam-4823 175 8 , , PUNCT bam-4823 175 9 wadsworth wadsworth NOUN bam-4823 175 10 center center NOUN bam-4823 175 11 , , PUNCT bam-4823 175 12 new new PROPN bam-4823 175 13 york york PROPN bam-4823 175 14 state state PROPN bam-4823 175 15 department department PROPN bam-4823 175 16 of of ADP bam-4823 175 17 health health NOUN bam-4823 175 18 , , PUNCT bam-4823 175 19 empire empire NOUN bam-4823 175 20 state state PROPN bam-4823 175 21 plaza plaza PROPN bam-4823 175 22 , , PUNCT bam-4823 175 23 p.o p.o PROPN bam-4823 175 24 . . PROPN bam-4823 175 25 box box PROPN bam-4823 175 26 509 509 NUM bam-4823 175 27 , , PUNCT bam-4823 175 28 albany albany PROPN bam-4823 175 29 , , PUNCT bam-4823 175 30 ny ny PROPN bam-4823 175 31 12201 12201 NUM bam-4823 175 32 - - SYM bam-4823 175 33 0509 0509 NUM bam-4823 175 34 e e NOUN bam-4823 175 35 - - NOUN bam-4823 175 36 mail mail NOUN bam-4823 175 37 : : PUNCT bam-4823 175 38 terence.wagenknecht@health.ny.gov terence.wagenknecht@health.ny.gov NUM bam-4823 175 39 chyongere chyongere ADJ bam-4823 175 40 hsieh hsieh PROPN bam-4823 175 41 : : PUNCT bam-4823 175 42 chongere.hsieh@health.ny.gov chongere.hsieh@health.ny.gov PROPN bam-4823 175 43 michael michael PROPN bam-4823 175 44 marko marko PROPN bam-4823 175 45 : : PUNCT bam-4823 175 46 michael.marko@health.ny.gov michael.marko@health.ny.gov NUM bam-4823 175 47 references reference NOUN bam-4823 175 48 1 1 NUM bam-4823 175 49 . . PUNCT bam-4823 175 50 franzini franzini PROPN bam-4823 175 51 - - PUNCT bam-4823 175 52 armstrong armstrong PROPN bam-4823 175 53 c. c. PROPN bam-4823 175 54 the the DET bam-4823 175 55 sarcoplasmic sarcoplasmic NOUN bam-4823 175 56 reticulum reticulum NOUN bam-4823 175 57 and and CCONJ bam-4823 175 58 the the DET bam-4823 175 59 control control NOUN bam-4823 175 60 of of ADP bam-4823 175 61 muscle muscle NOUN bam-4823 175 62 contraction contraction NOUN bam-4823 175 63 . . PUNCT bam-4823 176 1 faseb faseb PROPN bam-4823 176 2 journal journal PROPN bam-4823 176 3 1999;13:266 1999;13:266 PROPN bam-4823 176 4 - - SYM bam-4823 176 5 70 70 NUM bam-4823 176 6 . . PUNCT bam-4823 177 1 2 2 NUM bam-4823 177 2 . . NUM bam-4823 177 3 wagenknecht wagenknecht PROPN bam-4823 177 4 t t PROPN bam-4823 177 5 , , PUNCT bam-4823 177 6 hsieh hsieh PROPN bam-4823 177 7 ce ce PROPN bam-4823 177 8 , , PUNCT bam-4823 177 9 rath rath PROPN bam-4823 177 10 bk bk PROPN bam-4823 177 11 , , PUNCT bam-4823 177 12 et et PROPN bam-4823 177 13 al al PROPN bam-4823 177 14 . . PROPN bam-4823 177 15 electron electron PROPN bam-4823 177 16 tomography tomography NOUN bam-4823 177 17 of of ADP bam-4823 177 18 frozen frozen ADJ bam-4823 177 19 - - PUNCT bam-4823 177 20 hydrated hydrated ADJ bam-4823 177 21 isolated isolated ADJ bam-4823 177 22 triad triad NOUN bam-4823 177 23 junctions junction NOUN bam-4823 177 24 . . PUNCT bam-4823 178 1 biophys biophy NOUN bam-4823 178 2 j j PROPN bam-4823 178 3 2002;83:2491 2002;83:2491 NUM bam-4823 178 4 - - SYM bam-4823 178 5 501 501 NUM bam-4823 178 6 . . PUNCT bam-4823 179 1 3 3 X bam-4823 179 2 . . X bam-4823 179 3 hamilton hamilton PROPN bam-4823 179 4 sl sl PROPN bam-4823 179 5 , , PUNCT bam-4823 179 6 serysheva serysheva PROPN bam-4823 179 7 ii ii PROPN bam-4823 179 8 . . PROPN bam-4823 179 9 ryanodine ryanodine PROPN bam-4823 179 10 receptor receptor NOUN bam-4823 179 11 structure structure NOUN bam-4823 179 12 : : PUNCT bam-4823 179 13 progress progress NOUN bam-4823 179 14 and and CCONJ bam-4823 179 15 challenges challenge NOUN bam-4823 179 16 . . PUNCT bam-4823 180 1 j. j. PROPN bam-4823 180 2 biol biol PROPN bam-4823 180 3 . . PUNCT bam-4823 181 1 chem chem NOUN bam-4823 181 2 2009 2009 NUM bam-4823 181 3 , , PUNCT bam-4823 181 4 284 284 NUM bam-4823 181 5 , , PUNCT bam-4823 181 6 4047 4047 NUM bam-4823 181 7 - - SYM bam-4823 181 8 51 51 NUM bam-4823 181 9 . . NOUN bam-4823 181 10 4 4 NUM bam-4823 181 11 . . X bam-4823 182 1 wang wang PROPN bam-4823 182 2 mc mc PROPN bam-4823 182 3 , , PUNCT bam-4823 182 4 dolphin dolphin PROPN bam-4823 182 5 a a PRON bam-4823 182 6 , , PUNCT bam-4823 182 7 kitmitto kitmitto PROPN bam-4823 182 8 a. a. PROPN bam-4823 182 9 l l PROPN bam-4823 182 10 - - PUNCT bam-4823 182 11 type type NOUN bam-4823 182 12 voltage voltage NOUN bam-4823 182 13 - - PUNCT bam-4823 182 14 gated gate VERB bam-4823 182 15 calcium calcium NOUN bam-4823 182 16 channels channel NOUN bam-4823 182 17 : : PUNCT bam-4823 182 18 understanding understand VERB bam-4823 182 19 function function NOUN bam-4823 182 20 through through ADP bam-4823 182 21 structure structure NOUN bam-4823 182 22 . . PUNCT bam-4823 183 1 febs febs PROPN bam-4823 183 2 lett lett PROPN bam-4823 183 3 2004;564:245 2004;564:245 NUM bam-4823 183 4 - - SYM bam-4823 183 5 50 50 NUM bam-4823 183 6 . . PUNCT bam-4823 183 7 5 5 NUM bam-4823 183 8 . . X bam-4823 183 9 briggs briggs PROPN bam-4823 183 10 jag jag PROPN bam-4823 183 11 . . PUNCT bam-4823 184 1 structural structural ADJ bam-4823 184 2 biology biology NOUN bam-4823 184 3 in in ADP bam-4823 184 4 situ situ NOUN bam-4823 184 5 the the DET bam-4823 184 6 potential potential NOUN bam-4823 184 7 of of ADP bam-4823 184 8 subtomogram subtomogram NOUN bam-4823 184 9 averaging averaging NOUN bam-4823 184 10 . . PUNCT bam-4823 185 1 curr curr PROPN bam-4823 185 2 opinion opinion PROPN bam-4823 185 3 struct struct NOUN bam-4823 185 4 biol biol PROPN bam-4823 185 5 2013;23:261 2013;23:261 PROPN bam-4823 185 6 - - PUNCT bam-4823 185 7 7 7 NUM bam-4823 185 8 . . NOUN bam-4823 185 9 6 6 NUM bam-4823 185 10 . . X bam-4823 186 1 hoenger hoenger NOUN bam-4823 186 2 a. a. PROPN bam-4823 186 3 high high ADJ bam-4823 186 4 - - PUNCT bam-4823 186 5 resolution resolution NOUN bam-4823 186 6 cryo cryo NOUN bam-4823 186 7 - - PUNCT bam-4823 186 8 electron electron NOUN bam-4823 186 9 microscopy microscopy NOUN bam-4823 186 10 on on ADP bam-4823 186 11 macromolecular macromolecular NOUN bam-4823 186 12 complexes complex NOUN bam-4823 186 13 and and CCONJ bam-4823 186 14 cell cell NOUN bam-4823 186 15 organelles organelle NOUN bam-4823 186 16 . . PUNCT bam-4823 187 1 protoplasma protoplasma NOUN bam-4823 187 2 2014;251:417 2014;251:417 NUM bam-4823 187 3 - - SYM bam-4823 187 4 27 27 NUM bam-4823 187 5 . . PUNCT bam-4823 188 1 7 7 X bam-4823 188 2 . . X bam-4823 188 3 mcdowall mcdowall PROPN bam-4823 189 1 aw aw INTJ bam-4823 189 2 , , PUNCT bam-4823 189 3 chang chang PROPN bam-4823 189 4 jj jj PROPN bam-4823 189 5 , , PUNCT bam-4823 189 6 freeman freeman PROPN bam-4823 189 7 r. r. PROPN bam-4823 189 8 , , PUNCT bam-4823 189 9 et et PROPN bam-4823 189 10 al al PROPN bam-4823 189 11 . . PROPN bam-4823 189 12 electron electron PROPN bam-4823 189 13 microscopy microscopy NOUN bam-4823 189 14 of of ADP bam-4823 189 15 frozen frozen ADJ bam-4823 189 16 hydrated hydrated ADJ bam-4823 189 17 sections section NOUN bam-4823 189 18 of of ADP bam-4823 189 19 vitreous vitreous ADJ bam-4823 189 20 ice ice NOUN bam-4823 189 21 and and CCONJ bam-4823 189 22 vitrified vitrify VERB bam-4823 189 23 biological biological ADJ bam-4823 189 24 samples sample NOUN bam-4823 189 25 . . PUNCT bam-4823 190 1 j j PROPN bam-4823 190 2 microsc microsc PROPN bam-4823 190 3 1983;131:1 1983;131:1 NUM bam-4823 190 4 - - SYM bam-4823 190 5 9 9 NUM bam-4823 190 6 . . NOUN bam-4823 190 7 8 8 NUM bam-4823 190 8 . . PUNCT bam-4823 191 1 al al PROPN bam-4823 191 2 - - PUNCT bam-4823 191 3 amoudi amoudi PROPN bam-4823 191 4 a a PRON bam-4823 191 5 , , PUNCT bam-4823 191 6 dubochet dubochet PROPN bam-4823 191 7 j j PROPN bam-4823 191 8 , , PUNCT bam-4823 191 9 gnaegi gnaegi PROPN bam-4823 191 10 h h NOUN bam-4823 191 11 , , PUNCT bam-4823 191 12 et et PROPN bam-4823 191 13 al al PROPN bam-4823 191 14 . . PUNCT bam-4823 192 1 an an DET bam-4823 192 2 oscillating oscillate VERB bam-4823 192 3 cryo cryo NOUN bam-4823 192 4 - - PUNCT bam-4823 192 5 knife knife NOUN bam-4823 192 6 reduces reduce VERB bam-4823 192 7 cutting cut VERB bam-4823 192 8 - - PUNCT bam-4823 192 9 induced induce VERB bam-4823 192 10 deformation deformation NOUN bam-4823 192 11 of of ADP bam-4823 192 12 vitreous vitreous ADJ bam-4823 192 13 ultrathin ultrathin ADJ bam-4823 192 14 sections section NOUN bam-4823 192 15 . . PUNCT bam-4823 193 1 j j PROPN bam-4823 193 2 microsc microsc PROPN bam-4823 193 3 2003;212:26 2003;212:26 NUM bam-4823 193 4 - - SYM bam-4823 193 5 33 33 NUM bam-4823 193 6 . . NOUN bam-4823 193 7 9 9 NUM bam-4823 193 8 . . X bam-4823 194 1 al al PROPN bam-4823 194 2 - - PUNCT bam-4823 194 3 amoudi amoudi PROPN bam-4823 194 4 a a PRON bam-4823 194 5 , , PUNCT bam-4823 194 6 norlean norlean PROPN bam-4823 194 7 lpo lpo PROPN bam-4823 194 8 , , PUNCT bam-4823 194 9 dubochet dubochet NOUN bam-4823 194 10 j. j. PROPN bam-4823 194 11 cryoelectron cryoelectron PROPN bam-4823 194 12 microscopy microscopy NOUN bam-4823 194 13 of of ADP bam-4823 194 14 vitreous vitreous ADJ bam-4823 194 15 sections section NOUN bam-4823 194 16 of of ADP bam-4823 194 17 native native ADJ bam-4823 194 18 biological biological ADJ bam-4823 194 19 cells cell NOUN bam-4823 194 20 and and CCONJ bam-4823 194 21 tissues tissue NOUN bam-4823 194 22 . . PUNCT bam-4823 195 1 j j PROPN bam-4823 195 2 struct struct VERB bam-4823 195 3 biol biol PROPN bam-4823 195 4 2004;148:131 2004;148:131 NUM bam-4823 195 5 - - SYM bam-4823 195 6 5 5 NUM bam-4823 195 7 . . NOUN bam-4823 195 8 10 10 NUM bam-4823 195 9 . . PUNCT bam-4823 196 1 al al PROPN bam-4823 196 2 - - PUNCT bam-4823 196 3 amoudi amoudi PROPN bam-4823 196 4 a a PRON bam-4823 196 5 , , PUNCT bam-4823 196 6 studer studer NOUN bam-4823 196 7 d d PROPN bam-4823 196 8 , , PUNCT bam-4823 196 9 dubochet dubochet NOUN bam-4823 196 10 j. j. PROPN bam-4823 196 11 cutting cut VERB bam-4823 196 12 artefacts artefact NOUN bam-4823 196 13 and and CCONJ bam-4823 196 14 cutting cut VERB bam-4823 196 15 process process NOUN bam-4823 196 16 in in ADP bam-4823 196 17 vitreous vitreous ADJ bam-4823 196 18 sections section NOUN bam-4823 196 19 for for ADP bam-4823 196 20 cryo cryo NOUN bam-4823 196 21 - - PUNCT bam-4823 196 22 electron electron NOUN bam-4823 196 23 microscopy microscopy NOUN bam-4823 196 24 . . PUNCT bam-4823 197 1 j j PROPN bam-4823 197 2 struct struct NOUN bam-4823 197 3 biol biol PROPN bam-4823 197 4 2005;150:109 2005;150:109 NUM bam-4823 197 5 - - SYM bam-4823 197 6 21 21 NUM bam-4823 197 7 . . PUNCT bam-4823 198 1 mailto:terence.wagenknecht@health.ny.gov mailto:terence.wagenknecht@health.ny.gov ADJ bam-4823 198 2 skeletal skeletal ADJ bam-4823 198 3 muscle muscle NOUN bam-4823 198 4 triad triad VERB bam-4823 198 5 by by ADP bam-4823 198 6 fib fib NOUN bam-4823 198 7 milling milling NOUN bam-4823 198 8 and and CCONJ bam-4823 198 9 cryo cryo NOUN bam-4823 198 10 - - PUNCT bam-4823 198 11 et et PROPN bam-4823 198 12 eur eur PROPN bam-4823 198 13 j j PROPN bam-4823 198 14 transl transl PROPN bam-4823 198 15 myol myol PROPN bam-4823 198 16 basic basic ADJ bam-4823 198 17 appl appl NOUN bam-4823 198 18 myol myol NOUN bam-4823 198 19 2015 2015 NUM bam-4823 198 20 ; ; PUNCT bam-4823 198 21 25 25 NUM bam-4823 198 22 ( ( PUNCT bam-4823 198 23 1 1 NUM bam-4823 198 24 ): ): PUNCT bam-4823 198 25 49 49 NUM bam-4823 198 26 - - SYM bam-4823 198 27 56 56 NUM bam-4823 198 28 56 56 NUM bam-4823 198 29 11 11 NUM bam-4823 198 30 . . PUNCT bam-4823 199 1 hsieh hsieh PROPN bam-4823 199 2 ce ce PROPN bam-4823 199 3 , , PUNCT bam-4823 199 4 leith leith PROPN bam-4823 199 5 a a PROPN bam-4823 199 6 , , PUNCT bam-4823 199 7 mannella mannella NOUN bam-4823 199 8 ca can AUX bam-4823 199 9 , , PUNCT bam-4823 199 10 et et PROPN bam-4823 199 11 al al PROPN bam-4823 199 12 . . PROPN bam-4823 200 1 towards towards ADP bam-4823 200 2 high high ADJ bam-4823 200 3 - - PUNCT bam-4823 200 4 resolution resolution NOUN bam-4823 200 5 three three NUM bam-4823 200 6 - - PUNCT bam-4823 200 7 dimensional dimensional ADJ bam-4823 200 8 imaging imaging NOUN bam-4823 200 9 of of ADP bam-4823 200 10 native native ADJ bam-4823 200 11 mammalian mammalian ADJ bam-4823 200 12 tissue tissue NOUN bam-4823 200 13 : : PUNCT bam-4823 200 14 electron electron NOUN bam-4823 200 15 tomography tomography NOUN bam-4823 200 16 of of ADP bam-4823 200 17 frozen frozen ADJ bam-4823 200 18 - - PUNCT bam-4823 200 19 hydrated hydrated ADJ bam-4823 200 20 rat rat NOUN bam-4823 200 21 liver liver NOUN bam-4823 200 22 sections section NOUN bam-4823 200 23 . . PUNCT bam-4823 201 1 j j PROPN bam-4823 201 2 struct struct NOUN bam-4823 201 3 biol biol PROPN bam-4823 201 4 2006;153 2006;153 NUM bam-4823 201 5 : : PUNCT bam-4823 201 6 1 1 NUM bam-4823 201 7 - - SYM bam-4823 201 8 13 13 NUM bam-4823 201 9 . . PUNCT bam-4823 201 10 12 12 NUM bam-4823 201 11 . . PUNCT bam-4823 202 1 pierson pierson PROPN bam-4823 202 2 j j PROPN bam-4823 202 3 , , PUNCT bam-4823 202 4 fernandez fernandez PROPN bam-4823 202 5 jj jj PROPN bam-4823 202 6 , , PUNCT bam-4823 202 7 bos bos PROPN bam-4823 202 8 e e PROPN bam-4823 202 9 , , PUNCT bam-4823 202 10 et et PROPN bam-4823 202 11 al al PROPN bam-4823 202 12 . . PUNCT bam-4823 202 13 improving improve VERB bam-4823 202 14 the the DET bam-4823 202 15 technique technique NOUN bam-4823 202 16 of of ADP bam-4823 202 17 vitreous vitreous ADJ bam-4823 202 18 cryo cryo NOUN bam-4823 202 19 - - PUNCT bam-4823 202 20 sectioning section VERB bam-4823 202 21 for for ADP bam-4823 202 22 cryo cryo NOUN bam-4823 202 23 - - PUNCT bam-4823 202 24 electron electron NOUN bam-4823 202 25 tomography tomography NOUN bam-4823 202 26 : : PUNCT bam-4823 202 27 electrostatic electrostatic ADJ bam-4823 202 28 charging charging NOUN bam-4823 202 29 for for ADP bam-4823 202 30 section section NOUN bam-4823 202 31 attachment attachment NOUN bam-4823 202 32 and and CCONJ bam-4823 202 33 implementation implementation NOUN bam-4823 202 34 of of ADP bam-4823 202 35 an an DET bam-4823 202 36 anti anti ADJ bam-4823 202 37 - - ADJ bam-4823 202 38 contamination contamination ADJ bam-4823 202 39 glove glove NOUN bam-4823 202 40 box box PROPN bam-4823 202 41 . . PUNCT bam-4823 203 1 j j PROPN bam-4823 203 2 struct struct VERB bam-4823 203 3 biol biol NOUN bam-4823 203 4 2010;169:219 2010;169:219 NUM bam-4823 203 5 - - SYM bam-4823 203 6 25 25 NUM bam-4823 203 7 . . NOUN bam-4823 203 8 13 13 NUM bam-4823 203 9 . . PUNCT bam-4823 204 1 hsieh hsieh PROPN bam-4823 204 2 c c PROPN bam-4823 204 3 , , PUNCT bam-4823 204 4 schmelzer schmelzer PROPN bam-4823 204 5 t t PROPN bam-4823 204 6 , , PUNCT bam-4823 204 7 kishchenko kishchenko PROPN bam-4823 204 8 g g PROPN bam-4823 204 9 , , PUNCT bam-4823 204 10 et et PROPN bam-4823 204 11 al al PROPN bam-4823 204 12 . . PROPN bam-4823 205 1 practical practical ADJ bam-4823 205 2 workflow workflow NOUN bam-4823 205 3 for for ADP bam-4823 205 4 cryo cryo NOUN bam-4823 205 5 focused focus VERB bam-4823 205 6 - - PUNCT bam-4823 205 7 ion ion NOUN bam-4823 205 8 - - PUNCT bam-4823 205 9 beam beam NOUN bam-4823 205 10 milling milling NOUN bam-4823 205 11 of of ADP bam-4823 205 12 tissues tissue NOUN bam-4823 205 13 and and CCONJ bam-4823 205 14 cells cell NOUN bam-4823 205 15 for for ADP bam-4823 205 16 cryo cryo NOUN bam-4823 205 17 - - PUNCT bam-4823 205 18 tem tem PROPN bam-4823 205 19 tomography tomography NOUN bam-4823 205 20 . . PUNCT bam-4823 206 1 j j PROPN bam-4823 206 2 struct struct VERB bam-4823 206 3 biol biol PROPN bam-4823 206 4 2014;185:32 2014;185:32 PROPN bam-4823 206 5 - - PUNCT bam-4823 206 6 41 41 NUM bam-4823 206 7 . . PUNCT bam-4823 207 1 14 14 NUM bam-4823 207 2 . . PUNCT bam-4823 208 1 marko marko PROPN bam-4823 208 2 m m PROPN bam-4823 208 3 , , PUNCT bam-4823 208 4 hsieh hsieh PROPN bam-4823 208 5 c c PROPN bam-4823 208 6 , , PUNCT bam-4823 208 7 moberlychan moberlychan PROPN bam-4823 208 8 wj wj PROPN bam-4823 208 9 , , PUNCT bam-4823 208 10 et et PROPN bam-4823 208 11 al al PROPN bam-4823 208 12 . . PUNCT bam-4823 209 1 feasibility feasibility NOUN bam-4823 209 2 of of ADP bam-4823 209 3 focused focused ADJ bam-4823 209 4 ion ion NOUN bam-4823 209 5 beam beam NOUN bam-4823 209 6 miling mile VERB bam-4823 209 7 for for ADP bam-4823 209 8 preparation preparation NOUN bam-4823 209 9 of of ADP bam-4823 209 10 tem tem PROPN bam-4823 209 11 specimens specimen NOUN bam-4823 209 12 of of ADP bam-4823 209 13 biological biological ADJ bam-4823 209 14 material material NOUN bam-4823 209 15 embedded embed VERB bam-4823 209 16 in in ADP bam-4823 209 17 vitreous vitreous ADJ bam-4823 209 18 ice ice NOUN bam-4823 209 19 . . PUNCT bam-4823 210 1 microsc microsc ADJ bam-4823 210 2 microanal microanal ADJ bam-4823 210 3 2005 2005 NUM bam-4823 210 4 ; ; PUNCT bam-4823 210 5 11 11 NUM bam-4823 210 6 ( ( PUNCT bam-4823 210 7 suppl suppl NOUN bam-4823 210 8 2 2 NUM bam-4823 210 9 ): ): PUNCT bam-4823 210 10 802c-802d 802c-802d NUM bam-4823 210 11 . . X bam-4823 210 12 15 15 NUM bam-4823 210 13 . . PUNCT bam-4823 211 1 marko marko PROPN bam-4823 211 2 m m PROPN bam-4823 211 3 , , PUNCT bam-4823 211 4 hsieh hsieh PROPN bam-4823 211 5 c c NOUN bam-4823 211 6 , , PUNCT bam-4823 211 7 schalek schalek NOUN bam-4823 211 8 r r NOUN bam-4823 211 9 , , PUNCT bam-4823 211 10 et et PROPN bam-4823 211 11 al al PROPN bam-4823 211 12 . . PROPN bam-4823 211 13 focused focus VERB bam-4823 211 14 - - PUNCT bam-4823 211 15 ionbeam ionbeam NOUN bam-4823 211 16 thinning thinning NOUN bam-4823 211 17 of of ADP bam-4823 211 18 frozen frozen ADJ bam-4823 211 19 - - PUNCT bam-4823 211 20 hydrated hydrated ADJ bam-4823 211 21 biological biological ADJ bam-4823 211 22 specimens specimen NOUN bam-4823 211 23 for for ADP bam-4823 211 24 cryo cryo NOUN bam-4823 211 25 - - PUNCT bam-4823 211 26 electron electron NOUN bam-4823 211 27 microscopy microscopy NOUN bam-4823 211 28 . . PUNCT bam-4823 212 1 nature nature NOUN bam-4823 212 2 methods method NOUN bam-4823 212 3 2007;4:215 2007;4:215 NOUN bam-4823 212 4 - - SYM bam-4823 212 5 17 17 NUM bam-4823 212 6 . . PUNCT bam-4823 213 1 16 16 NUM bam-4823 213 2 . . PUNCT bam-4823 214 1 marko marko PROPN bam-4823 214 2 m m PROPN bam-4823 214 3 , , PUNCT bam-4823 214 4 hsieh hsieh PROPN bam-4823 214 5 c c NOUN bam-4823 214 6 , , PUNCT bam-4823 214 7 moberlychan moberlychan PROPN bam-4823 214 8 w w PROPN bam-4823 214 9 , , PUNCT bam-4823 214 10 et et PROPN bam-4823 214 11 al al PROPN bam-4823 214 12 . . PROPN bam-4823 214 13 focused focus VERB bam-4823 214 14 ion ion NOUN bam-4823 214 15 beam beam NOUN bam-4823 214 16 milling milling NOUN bam-4823 214 17 of of ADP bam-4823 214 18 vitreous vitreous ADJ bam-4823 214 19 water water NOUN bam-4823 214 20 : : PUNCT bam-4823 214 21 prospects prospect NOUN bam-4823 214 22 for for ADP bam-4823 214 23 an an DET bam-4823 214 24 alternative alternative NOUN bam-4823 214 25 to to PART bam-4823 214 26 cryoultramicrotomy cryoultramicrotomy VERB bam-4823 214 27 of of ADP bam-4823 214 28 frozen frozen ADJ bam-4823 214 29 - - PUNCT bam-4823 214 30 hydrated hydrated ADJ bam-4823 214 31 biological biological ADJ bam-4823 214 32 samples sample NOUN bam-4823 214 33 . . PUNCT bam-4823 215 1 j j PROPN bam-4823 215 2 microsc microsc PROPN bam-4823 215 3 2006;222 2006;222 NUM bam-4823 215 4 : : PUNCT bam-4823 215 5 42 42 NUM bam-4823 215 6 - - SYM bam-4823 215 7 47 47 NUM bam-4823 215 8 . . PUNCT bam-4823 215 9 17 17 NUM bam-4823 215 10 . . PUNCT bam-4823 216 1 kizilyaprak kizilyaprak PROPN bam-4823 216 2 c c PROPN bam-4823 216 3 , , PUNCT bam-4823 216 4 daraspe daraspe PROPN bam-4823 216 5 j j PROPN bam-4823 216 6 , , PUNCT bam-4823 216 7 humbel humbel PROPN bam-4823 216 8 bm bm PROPN bam-4823 216 9 . . PROPN bam-4823 216 10 focused focus VERB bam-4823 216 11 ion ion NOUN bam-4823 216 12 beam beam NOUN bam-4823 216 13 scanning scan VERB bam-4823 216 14 electron electron NOUN bam-4823 216 15 microscopy microscopy NOUN bam-4823 216 16 in in ADP bam-4823 216 17 biology biology NOUN bam-4823 216 18 . . PUNCT bam-4823 217 1 j j PROPN bam-4823 217 2 microsc microsc PROPN bam-4823 217 3 2014;254:109 2014;254:109 NUM bam-4823 217 4 - - SYM bam-4823 217 5 14 14 NUM bam-4823 217 6 . . PUNCT bam-4823 217 7 18 18 NUM bam-4823 217 8 . . PUNCT bam-4823 218 1 villa villa PROPN bam-4823 218 2 e e PROPN bam-4823 218 3 , , PUNCT bam-4823 218 4 schaffer schaffer PROPN bam-4823 218 5 m m PROPN bam-4823 218 6 , , PUNCT bam-4823 218 7 plitzko plitzko PROPN bam-4823 218 8 jm jm PROPN bam-4823 218 9 , , PUNCT bam-4823 218 10 baumeister baumeister PROPN bam-4823 218 11 w. w. PROPN bam-4823 218 12 opening open VERB bam-4823 218 13 windows window NOUN bam-4823 218 14 into into ADP bam-4823 218 15 the the DET bam-4823 218 16 cell cell NOUN bam-4823 218 17 : : PUNCT bam-4823 218 18 focused focused ADJ bam-4823 218 19 - - PUNCT bam-4823 218 20 ion ion NOUN bam-4823 218 21 - - PUNCT bam-4823 218 22 beam beam NOUN bam-4823 218 23 milling milling NOUN bam-4823 218 24 for for ADP bam-4823 218 25 cryo cryo NOUN bam-4823 218 26 - - PUNCT bam-4823 218 27 electron electron NOUN bam-4823 218 28 tomography tomography NOUN bam-4823 218 29 . . PUNCT bam-4823 219 1 curr curr PROPN bam-4823 219 2 opin opin PROPN bam-4823 219 3 struct struct VERB bam-4823 219 4 biol biol NOUN bam-4823 219 5 2013;23:771 2013;23:771 NOUN bam-4823 219 6 - - SYM bam-4823 219 7 7 7 NUM bam-4823 219 8 . . NOUN bam-4823 219 9 19 19 NUM bam-4823 219 10 . . PUNCT bam-4823 220 1 rigort rigort PROPN bam-4823 220 2 a a PROPN bam-4823 220 3 , , PUNCT bam-4823 220 4 bauerlein bauerlein NOUN bam-4823 220 5 fjb fjb NOUN bam-4823 220 6 , , PUNCT bam-4823 220 7 leis leis NOUN bam-4823 220 8 a a PRON bam-4823 220 9 , , PUNCT bam-4823 220 10 et et PROPN bam-4823 220 11 al al PROPN bam-4823 220 12 . . PUNCT bam-4823 221 1 micromachining micromachine VERB bam-4823 221 2 tools tool NOUN bam-4823 221 3 and and CCONJ bam-4823 221 4 correlative correlative ADJ bam-4823 221 5 approaches approach NOUN bam-4823 221 6 for for ADP bam-4823 221 7 cellular cellular ADJ bam-4823 221 8 cryo cryo NOUN bam-4823 221 9 - - PUNCT bam-4823 221 10 electron electron NOUN bam-4823 221 11 tomography tomography NOUN bam-4823 221 12 . . PUNCT bam-4823 222 1 j j PROPN bam-4823 222 2 struct struct VERB bam-4823 222 3 biol biol PROPN bam-4823 222 4 2010;172:169 2010;172:169 PROPN bam-4823 222 5 - - SYM bam-4823 222 6 79 79 NUM bam-4823 222 7 . . PUNCT bam-4823 223 1 20 20 NUM bam-4823 223 2 . . PUNCT bam-4823 224 1 rigort rigort PROPN bam-4823 224 2 a a PROPN bam-4823 224 3 , , PUNCT bam-4823 224 4 gunther gunther PROPN bam-4823 224 5 d d PROPN bam-4823 224 6 , , PUNCT bam-4823 224 7 hegerl hegerl NOUN bam-4823 224 8 r r NOUN bam-4823 224 9 , , PUNCT bam-4823 224 10 et et PROPN bam-4823 224 11 al al PROPN bam-4823 224 12 . . PROPN bam-4823 224 13 automated automate VERB bam-4823 224 14 segmentation segmentation NOUN bam-4823 224 15 of of ADP bam-4823 224 16 electron electron NOUN bam-4823 224 17 tomograms tomogram NOUN bam-4823 224 18 for for ADP bam-4823 224 19 a a DET bam-4823 224 20 quantitative quantitative ADJ bam-4823 224 21 description description NOUN bam-4823 224 22 of of ADP bam-4823 224 23 actin actin NOUN bam-4823 224 24 filament filament NOUN bam-4823 224 25 networks network NOUN bam-4823 224 26 . . PUNCT bam-4823 225 1 j j PROPN bam-4823 225 2 struct struct VERB bam-4823 225 3 biol biol PROPN bam-4823 225 4 2012;177:135 2012;177:135 NUM bam-4823 225 5 - - SYM bam-4823 225 6 44 44 NUM bam-4823 225 7 . . PUNCT bam-4823 226 1 21 21 NUM bam-4823 226 2 . . PUNCT bam-4823 226 3 rigort rigort PROPN bam-4823 226 4 a a PROPN bam-4823 226 5 , , PUNCT bam-4823 226 6 villa villa PROPN bam-4823 226 7 e e PROPN bam-4823 226 8 , , PUNCT bam-4823 226 9 bauerlein bauerlein NOUN bam-4823 226 10 fjb fjb NOUN bam-4823 226 11 , , PUNCT bam-4823 226 12 et et PROPN bam-4823 226 13 al al PROPN bam-4823 226 14 . . PROPN bam-4823 226 15 integrative integrative ADJ bam-4823 226 16 approaches approach NOUN bam-4823 226 17 for for ADP bam-4823 226 18 cellular cellular ADJ bam-4823 226 19 cryo cryo NOUN bam-4823 226 20 - - PUNCT bam-4823 226 21 electron electron NOUN bam-4823 226 22 tomography tomography NOUN bam-4823 226 23 : : PUNCT bam-4823 227 1 correlative correlative ADJ bam-4823 227 2 imaging imaging NOUN bam-4823 227 3 and and CCONJ bam-4823 227 4 focused focused ADJ bam-4823 227 5 ion ion NOUN bam-4823 227 6 beam beam NOUN bam-4823 227 7 micromachining micromachining NOUN bam-4823 227 8 . . PUNCT bam-4823 228 1 methods method NOUN bam-4823 228 2 in in ADP bam-4823 228 3 cell cell NOUN bam-4823 228 4 biol biol NOUN bam-4823 228 5 2012;111:259 2012;111:259 NUM bam-4823 228 6 - - SYM bam-4823 228 7 81 81 NUM bam-4823 228 8 . . PUNCT bam-4823 229 1 22 22 NUM bam-4823 229 2 . . PUNCT bam-4823 230 1 strunk strunk NOUN bam-4823 230 2 km km PROPN bam-4823 230 3 , , PUNCT bam-4823 230 4 wang wang PROPN bam-4823 230 5 k k PROPN bam-4823 230 6 , , PUNCT bam-4823 230 7 ke ke PROPN bam-4823 230 8 d d PROPN bam-4823 230 9 , , PUNCT bam-4823 230 10 gray gray ADJ bam-4823 230 11 jl jl PROPN bam-4823 230 12 , , PUNCT bam-4823 230 13 zhang zhang PROPN bam-4823 230 14 p. p. PROPN bam-4823 230 15 thinning thinning NOUN bam-4823 230 16 of of ADP bam-4823 230 17 large large ADJ bam-4823 230 18 mammalian mammalian ADJ bam-4823 230 19 cells cell NOUN bam-4823 230 20 for for ADP bam-4823 230 21 cryo cryo NOUN bam-4823 230 22 - - PUNCT bam-4823 230 23 tem tem PROPN bam-4823 230 24 characterization characterization NOUN bam-4823 230 25 by by ADP bam-4823 230 26 cryo cryo NOUN bam-4823 230 27 - - PUNCT bam-4823 230 28 fib fib NOUN bam-4823 230 29 milling milling NOUN bam-4823 230 30 . . PUNCT bam-4823 231 1 j j PROPN bam-4823 231 2 microsc microsc PROPN bam-4823 231 3 2012;247:220 2012;247:220 NUM bam-4823 231 4 - - SYM bam-4823 231 5 7 7 NUM bam-4823 231 6 . . NOUN bam-4823 231 7 23 23 NUM bam-4823 231 8 . . PUNCT bam-4823 232 1 wang wang PROPN bam-4823 232 2 k k PROPN bam-4823 232 3 , , PUNCT bam-4823 232 4 strunk strunk PROPN bam-4823 232 5 k k PROPN bam-4823 232 6 , , PUNCT bam-4823 232 7 zhao zhao PROPN bam-4823 232 8 gp gp NOUN bam-4823 232 9 , , PUNCT bam-4823 232 10 et et PROPN bam-4823 232 11 al al PROPN bam-4823 232 12 . . PROPN bam-4823 233 1 3d 3d NUM bam-4823 233 2 structure structure NOUN bam-4823 233 3 determination determination NOUN bam-4823 233 4 of of ADP bam-4823 233 5 native native ADJ bam-4823 233 6 mammalian mammalian ADJ bam-4823 233 7 cells cell NOUN bam-4823 233 8 using use VERB bam-4823 233 9 cryo cryo NOUN bam-4823 233 10 - - PUNCT bam-4823 233 11 fib fib ADJ bam-4823 233 12 and and CCONJ bam-4823 233 13 cryo cryo NOUN bam-4823 233 14 - - PUNCT bam-4823 233 15 electron electron NOUN bam-4823 233 16 tomography tomography NOUN bam-4823 233 17 . . PUNCT bam-4823 234 1 j j PROPN bam-4823 234 2 struct struct VERB bam-4823 234 3 biol biol PROPN bam-4823 234 4 2012;180:318 2012;180:318 PROPN bam-4823 234 5 - - SYM bam-4823 234 6 26 26 NUM bam-4823 234 7 . . PUNCT bam-4823 234 8 24 24 NUM bam-4823 234 9 . . PUNCT bam-4823 235 1 franzini franzini PROPN bam-4823 235 2 - - PUNCT bam-4823 235 3 armstrong armstrong PROPN bam-4823 235 4 c c PROPN bam-4823 235 5 , , PUNCT bam-4823 235 6 nunzi nunzi PROPN bam-4823 235 7 g. g. PROPN bam-4823 235 8 junctional junctional ADJ bam-4823 235 9 feet foot NOUN bam-4823 235 10 and and CCONJ bam-4823 235 11 particles particle NOUN bam-4823 235 12 in in ADP bam-4823 235 13 the the DET bam-4823 235 14 triads triad NOUN bam-4823 235 15 of of ADP bam-4823 235 16 a a DET bam-4823 235 17 fast fast ADJ bam-4823 235 18 - - PUNCT bam-4823 235 19 twitch twitch NOUN bam-4823 235 20 muscle muscle NOUN bam-4823 235 21 fibre fibre NOUN bam-4823 235 22 . . PUNCT bam-4823 236 1 j j PROPN bam-4823 236 2 muscle muscle NOUN bam-4823 236 3 res re NOUN bam-4823 236 4 cell cell NOUN bam-4823 236 5 motil motil NOUN bam-4823 236 6 1983;4:233 1983;4:233 NUM bam-4823 236 7 - - SYM bam-4823 236 8 52 52 NUM bam-4823 236 9 . . PUNCT bam-4823 237 1 25 25 NUM bam-4823 237 2 . . PUNCT bam-4823 238 1 gan gan PROPN bam-4823 238 2 l l PROPN bam-4823 238 3 , , PUNCT bam-4823 238 4 jensen jensen PROPN bam-4823 238 5 gj gj PROPN bam-4823 238 6 . . PROPN bam-4823 238 7 electron electron PROPN bam-4823 238 8 tomography tomography NOUN bam-4823 238 9 of of ADP bam-4823 238 10 cells cell NOUN bam-4823 238 11 . . PUNCT bam-4823 239 1 q q PROPN bam-4823 239 2 rev rev PROPN bam-4823 239 3 of of ADP bam-4823 239 4 biophys biophy NOUN bam-4823 239 5 2012;45:27 2012;45:27 NUM bam-4823 239 6 - - SYM bam-4823 239 7 56 56 NUM bam-4823 239 8 . . NOUN bam-4823 239 9 26 26 NUM bam-4823 239 10 . . PUNCT bam-4823 239 11 hu hu PROPN bam-4823 240 1 b b PROPN bam-4823 240 2 , , PUNCT bam-4823 240 3 margolin margolin PROPN bam-4823 240 4 w w PROPN bam-4823 240 5 , , PUNCT bam-4823 240 6 molineux molineux PROPN bam-4823 240 7 ij ij NOUN bam-4823 240 8 , , PUNCT bam-4823 240 9 liu liu PROPN bam-4823 240 10 , , PUNCT bam-4823 240 11 j. j. PROPN bam-4823 240 12 the the DET bam-4823 240 13 bacteriophage bacteriophage PROPN bam-4823 240 14 t7 t7 PROPN bam-4823 240 15 virion virion NOUN bam-4823 240 16 undergoes undergo VERB bam-4823 240 17 extensive extensive ADJ bam-4823 240 18 structural structural ADJ bam-4823 240 19 remodeling remodeling NOUN bam-4823 240 20 during during ADP bam-4823 240 21 infection infection NOUN bam-4823 240 22 . . PUNCT bam-4823 241 1 science science NOUN bam-4823 241 2 2013;339:576 2013;339:576 NUM bam-4823 241 3 - - SYM bam-4823 241 4 9 9 NUM bam-4823 241 5 . . NOUN bam-4823 241 6 27 27 NUM bam-4823 241 7 . . PUNCT bam-4823 242 1 fukuda fukuda PROPN bam-4823 242 2 y y PROPN bam-4823 242 3 , , PUNCT bam-4823 242 4 schrod schrod NOUN bam-4823 242 5 n n CCONJ bam-4823 242 6 , , PUNCT bam-4823 242 7 schaffer schaffer PROPN bam-4823 242 8 m m PROPN bam-4823 242 9 , , PUNCT bam-4823 242 10 et et PROPN bam-4823 242 11 al al PROPN bam-4823 242 12 . . PROPN bam-4823 242 13 coordinate coordinate PROPN bam-4823 242 14 transformation transformation NOUN bam-4823 242 15 based base VERB bam-4823 242 16 cryo cryo NOUN bam-4823 242 17 - - PUNCT bam-4823 242 18 correlative correlative ADJ bam-4823 242 19 methods method NOUN bam-4823 242 20 for for ADP bam-4823 242 21 electron electron NOUN bam-4823 242 22 tomography tomography NOUN bam-4823 242 23 and and CCONJ bam-4823 242 24 focused focused ADJ bam-4823 242 25 ion ion NOUN bam-4823 242 26 beam beam NOUN bam-4823 242 27 milling milling NOUN bam-4823 242 28 . . PUNCT bam-4823 243 1 ultramicroscopy ultramicroscopy NOUN bam-4823 243 2 2014;143:15 2014;143:15 NUM bam-4823 243 3 - - SYM bam-4823 243 4 23 23 NUM bam-4823 243 5 . . PUNCT bam-4823 243 6 28 28 NUM bam-4823 243 7 . . PUNCT bam-4823 244 1 lucic lucic PROPN bam-4823 244 2 v v PROPN bam-4823 244 3 , , PUNCT bam-4823 244 4 rigort rigort NOUN bam-4823 244 5 a a NOUN bam-4823 244 6 , , PUNCT bam-4823 244 7 baumeister baumeister PROPN bam-4823 244 8 w. w. PROPN bam-4823 244 9 cryo cryo PROPN bam-4823 244 10 - - PUNCT bam-4823 244 11 electron electron NOUN bam-4823 244 12 tomography tomography NOUN bam-4823 244 13 : : PUNCT bam-4823 244 14 the the DET bam-4823 244 15 challenge challenge NOUN bam-4823 244 16 of of ADP bam-4823 244 17 doing do VERB bam-4823 244 18 structural structural ADJ bam-4823 244 19 biology biology NOUN bam-4823 244 20 in in ADP bam-4823 244 21 situ situ NOUN bam-4823 244 22 . . PUNCT bam-4823 245 1 j j PROPN bam-4823 245 2 cell cell NOUN bam-4823 245 3 biol biol PROPN bam-4823 245 4 2013;202:407 2013;202:407 NUM bam-4823 245 5 - - SYM bam-4823 245 6 19 19 NUM bam-4823 245 7 . . NOUN bam-4823 245 8 29 29 NUM bam-4823 245 9 . . X bam-4823 246 1 de de PROPN bam-4823 246 2 winter winter PROPN bam-4823 246 3 dam dam PROPN bam-4823 246 4 , , PUNCT bam-4823 246 5 mesman mesman PROPN bam-4823 246 6 rj rj PROPN bam-4823 246 7 , , PUNCT bam-4823 246 8 hayles hayles PROPN bam-4823 246 9 mf mf PROPN bam-4823 246 10 . . PROPN bam-4823 246 11 et et PROPN bam-4823 246 12 al al PROPN bam-4823 246 13 . . PROPN bam-4823 247 1 in in ADP bam-4823 247 2 - - PUNCT bam-4823 247 3 situ situ NOUN bam-4823 247 4 integrity integrity NOUN bam-4823 247 5 control control NOUN bam-4823 247 6 of of ADP bam-4823 247 7 frozen frozen ADJ bam-4823 247 8 - - PUNCT bam-4823 247 9 hydrated hydrated ADJ bam-4823 247 10 , , PUNCT bam-4823 247 11 vitreous vitreous ADJ bam-4823 247 12 lamellas lamella NOUN bam-4823 247 13 prepared prepare VERB bam-4823 247 14 by by ADP bam-4823 247 15 the the DET bam-4823 247 16 cryo cryo NOUN bam-4823 247 17 - - PUNCT bam-4823 247 18 focused focus VERB bam-4823 247 19 ion ion NOUN bam-4823 247 20 beam beam NOUN bam-4823 247 21 - - PUNCT bam-4823 247 22 scanning scan VERB bam-4823 247 23 electron electron NOUN bam-4823 247 24 microscope microscope NOUN bam-4823 247 25 . . PUNCT bam-4823 248 1 j j PROPN bam-4823 248 2 struct struct VERB bam-4823 248 3 biol biol PROPN bam-4823 248 4 2013;183:11 2013;183:11 NUM bam-4823 248 5 - - SYM bam-4823 248 6 8 8 NUM