Food Science and Nutrition Studies ISSN 2573-1661 (Print) ISSN 2573-167X (Online) Vol. 1, No. 2, 2017 www.scholink.org/ojs/index.php/fsns 61 Comparative Study of Starch Characteristics, In-Vitro Starch Digestibility and Glycemic Index of Some Starchy Foods Consumed in Nigeria Israel Olusegun Otemuyiwa1*, Adedayo Muideen Sanni1 & Emmanuel Ayorinde Oyewumi1 1 Department of Chemistry, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria * Israel Olusegun Otemuyiwa, E-mail: otemuyisegun@oauife.edu.ng Received: June 25, 2017 Accepted: July 8, 2017 Online Published: July 29, 2017 doi:10.22158/fsns.v1n2p61 URL: http://dx.doi.org/10.22158/fsns.v1n2p61 Abstract The study investigated the carbohydrate characteristics and in-vitro starch digestibility of some starchy food consumed in Nigeria. Ten foods samples (cassava, yam, red and white sorghum, rice, plantain, banana, semovita, noodles and bread) were selected. The content of starch, amylose and sugar were determined by colorimetric method, in vitro rate of starch hydrolysis was evaluated by multi-enzyme digestion method over a period of two hours, the glucose released was estimated by colorimetric method and was compared to the reference food (bread). The result showed that the percentage moisture content and total starch ranged from 9.8 to 15.3% and 236 to 248 mg/g, while amylose, Rapidly Digestible Starch (RDS) and Resistance Starch (RS) ranged from 8.41 to 19.2%, 30.8 to 51% and 7.8 to 37.4%, respectively. The in-vitro digestibility study indicated that the equilibrium Concentration (Cα), Kinetic constant (K ), Hydrolysis Index (HI) and Glycemic Index (GI) ranged from 34 to 64.9, and 0.02 to 0.07, 56.6 to 104 and 71 to 96.8, respectively. Positive correlations (P < 0.05) exist between RDS and GI (r = 0.700) and RS and amylose (r = 0.899) The study revealed that, structure of dietary carbohydrate could greatly influenced the Glycemic Index, plantain and noodles with low RDS and low hydrolysis constant may be beneficial in management of diabetes whereas sorghum, semovita, cassava and bread with high RDS, and high GI should be taken sparingly or combined with high protein and low glycemic load foods. Keywords Glycemic Index, Resistant Starch, amylose, in vitro digestion, Nigeria 1. Introduction In recent years it has become evident that significant health risks and benefits are associated with dietary food choice. A particular disease has been associated with carbohydrate foods with high Glycemic Index www.scholink.org/ojs/index.php/fsns Food Science and Nutrition Studies Vol. 1, No. 2, 2017 62 Published by SCHOLINK INC. and high digestibility rate. About 104 millions of people worldwide are being afflicted with the perfect epidemic known as the Diabetes. This figure is increasing daily. Diabetes meets all criteria for a public health disorder (Seal et al., 2003). Nutrition is a significant cornerstone of diabetes care as described in intensive management. The main focus in nutritional management of diabetes is to improve glycemic control by balancing food intake with endogenous and/or exogenous insulin level (Heacock et al., 2004). Historically, attempt has been made to control the glycemic response to food, particularly carbohydrate-containing foods, including use of very low carbohydrate and starvation diets, artificial sweeteners and pharmacological preparations such as fast acting insulin and inhibitors of carbohydrate absorption (Heacock et al., 2004). In Nigeria, the diet of the people is predominantly carbohydrate obtained from either root tuber or cereal grains. There are varieties of food that were consumed which complement one another without the empirical knowledge of their digestion rate and optimum intake that will give sufficient nutrient intake. Foods like carbohydrates for instance should be monitored carefully. There is the need to consider the rate at which these foods digest and be able to predict their Glycemic Index in order to prevent glucose induced ailment. One way to classify the glycemic response of various carbohydrate-containing foods is Glycemic Index (GI). The Glycemic Index (GI) is an in-vitro measurement based on glycemic response to carbohydrate-containing foods. The index allows ranking of carbohydrate foods on the basis of the rate of digestion and absorption (Jenkins et al., 1981; Englyst et al., 1992). In-vitro method has also been used to classify foods based on their digestion characteristics similar to the in vivo situation, and to identify slow release of carbohydrate in foods (Jenkins et al., 1984). The foods with GI values more than 70%, between 56% and 69% and lower than 55% were classified as high, medium, and low GI foods, respectively (Brand-Miller et al., 2003). The study carried out using human subject by Asinobi et al. (2016) to determine the blood sugar response of some traditional fortified staple meals in Nigeria concluded that unripe plantain had the lowest Glycemic Index value with lowest postprandial glucose response. Also Fasanmade and Anyakudo (2007) concluded that yam based food product should be generously used by diabetes patient because of its low Glycemic Index. These experiments were carried out under in-vivo conditions as such none of the researcher addressed the nature and characteristics of the starch present in foods analysed. The digestibility of starch in foods may vary widely (Björck et al., 1994). Hence, a nutritional classification of dietary starch has been proposed, which takes into account both the kinetic component and the completeness of its digestibility, thus comprising Rapidly Digestible (RDS), Slowly Digestible (SDS), and indigestible or resistant fractions (RS) (Englyst et al., 1992). The objectives of this study were to carry out in-vitro digestibility studies of some starchy staple diets consumed in Nigeria, determine the rate of hydrolysis and the starch content characteristics, and also predict the Glycemic Index. The study would provide an insight into the basic cause of epidemics associated with elevated glucose induced type 2-diabetes among Nigerians. www.scholink.org/ojs/index.php/fsns Food Science and Nutrition Studies Vol. 1, No. 2, 2017 63 Published by SCHOLINK INC. 2. Materials and Methods 2.1 Sample and Sample Preparation The samples selected for this study are yam flour, cassava flour, unripe plantain flour, unripe banana flour, flour of white and red sorghum, semovita, rice, noodles and bread. The samples were dried and milled using a locally fabricated mill (Lawood Metals, Osogbo, Nigeria). The milled samples were sieved using a local sieve (aperture size of 0.6 mm) to remove the coarser fragments. All the samples were milled as one batch, mixed thoroughly and sub-samples randomly taken from different parts of each milled sample, mixed together and stored in the freezer until analyzed. 2.2 Analysis of Proximate Composition The proximate composition of the samples (moisture, ash, Crude fibre) were determined by the method of AOAC (2000). 2.3 Determination of Total and Reducing Sugar Content Soluble sugar was extracted from 2.0 g sample with 85% ethanol using soxhlet extractor and refluxed for 2 h as described by Bambridge et al. (1996) Reducing sugar and total sugar were determined from the ethanolic extract by the ferricyanide method (AOAC, 1984). Glucose was used as a standard and the glucose content of the sample was calculated using a linear equation y = 1.6216 - 0.001x (R2 = 0.972). 2.4 Determination of Total Starch The total starch content of the samples was determined on the residue obtained after ethanolic extraction of sugar. Residue (200 mg) was refluxed with 0.7 M HCl for 2.5 h. The acid hydrolysate was neutralized to pH 7.0 using 5.0 M NaOH, pour into 500 mL standard flask and made up to volume with distilled water. The hydrolysate was filtered through a Whatman no. 541 filter paper and the starch was determined as the reducing sugar using the ferricyanide method (Bainbridge et al., 1996). The glucose content was calculated using a glucose standard linear equation and then converted to starch content using the AOAC (1984) equation. 2.5 Determination of Amylose Content Amylose content in rice samples were determined based on the Iodine-binding procedure as described by Thomas et al. (2013). The sample (100 mg) was measured into 100 mL standard flask, 1.0 ml of ethanol (95%) and 9.0 ml of 1.0 M NaOH were added, the mixture was heated on a boiling water bath for 10 min to gelatinize the starch. 5.0 ml of the gelatinized starch solution was transferred to a 100 ml standard flask, 1.0 mL of 1.0 M acetic acid and 2.0 ml of iodine solution were added and made up to volume with distilled water. All the contents were thoroughly vortex mixed and allowed to stand for 20 min. The absorbance was measured at 620 nm using a UV-Spectrophotometer (Model AA-6650, Shimadzu Co. Japan). The amylose content was calculated from the standard curve of potato amylose using the linear equation (R2 = 0.899). 2.6 In-Vitro Starch Hydrolysis The in-vitro starch digestibility was determined by multi-enzyme procedure described by Deepa et al. www.scholink.org/ojs/index.php/fsns Food Science and Nutrition Studies Vol. 1, No. 2, 2017 64 Published by SCHOLINK INC. (2010). The sample (250 mg) was gelatinized in 10 mL distilled water on a hot plate. The gelatinized sample was homogenized with 10 mL of HCl-KCl buffer (pH 1.5) using a basic homogenizer (Kika Labortechnik 725, Janke and Kukel GmbH & Co., Stanfen Germany) at 9500 rpm for 1 min and the homogenate was then digested with 20 mg of pepsin (Sigma; CAS 2001/75-6, code 10132561, 666 iu/mg, porcine gastric mucosa) solution (prepared by adding 1.0 g of pepsin/10 mL of HCl-KCl buffer) for 1 h in a shaking water bath at 37oC. The pH of the digestate was adjusted to 6.9 and the volume made to 25 ml using Tris-maleate buffer (pH 6.9). Then 5.0 mL of α-amylase (2.6 IU in 5 ml buffer pH 6.9) was added to the digestate which was incubated at 37oC in a shaking water bath. One ml of sample aliquots was collected at intervals of 30 min for 180 min, the enzyme activity in the aliquot withdrawn was inactivated by immediately placing the tube in a boiling water bath maintained at 100oC for 5 min and then refrigerated till the end of the incubation period, To these aliquots, 3 ml of 0.4 M sodium acetate buffer (pH 4.75) and 60 µl amyloglucosidase (Sigma, No;10105-5GF,70 ui/mg. Aspegilius niger) were added and incubated at 60oC for 5 min to hydrolyse the starch to glucose. The glucose released was determined using dinitrosalicylic acid (Miller, 1959). The concentration of glucose was calculated from the linear equation of glucose standard (R2 = 0.980) and glucose was converted into starch by multiplying with 0.9. All the experiments were conducted thrice and with triplicate analysis each. The rate of starch digestion was expressed as the percentage of TS hydrolyzed at different times. The digestibility curve for each food sample was fitted into the first-order equation (Grandfeidt et al., 1992). )1( kt t eCC    where Ct is the percentage of starch hydrolyzed at time t (min), C∞ is the equilibrium starch hydrolysis after 180 min, k is a pseudo-first order rate constant. The parameters, k and Cα were estimated for each sample based on the data obtained from starch hydrolysis procedure using Microsoft Excel Software. Hydrolysis Index (HI) was obtained by dividing the area under the hydrolysis curve of the sample by the corresponding area of a reference food (white bread) expressed as a percentage (Grandfeidt et al., 1992). Glycemic Index (GI) was estimated using the equation of Goni et al. (1997). )549.0(71.39 HIGI  2.7 Determination of Rapidly Digestible, Slowly Digestible and Resistance Starch The method of Han et al. (2007) was used to estimate the rapidly digestible, slowly digestible and resistance starch. The Rapidly Digestible Starch (RDS) is the fraction of starch hydrolyzed within 30 min of incubation, Slowly Digestible Starch (SDS) is the fraction hydrolyzed between 30 to 180 min and the fraction that remained un-hydrolyzed after 180 min is regarded as the Resistant Starch. 2.8 Statistical Analysis Analyses were carried out in triplicate for each determination and the results were expressed as mean www.scholink.org/ojs/index.php/fsns Food Science and Nutrition Studies Vol. 1, No. 2, 2017 65 Published by SCHOLINK INC. and standard deviation. The data were subjected to analysis of variance, and pearson correlation coefficient and the levels of significant difference was performed. GraphPad InStat version 3.06 for Windows 2003 was used for statistical analysis. 3. Results and Discussion The results of starch and sugar and chemical characteristics of the starch were presented in Table 1. The moisture content of the local foods ranged from 8.2 to 15.3%, the highest value was found in yam flour, these values compares with 12 to 14% predicted as optimum moisture content for storage of flour foods and for obtaining quality product during milling (Souilah et al., 2014). The fibre content ranged from 0.48% in rice to 4.9% in noodles. The fibre content of rice is expectedly low compared to others, this is because it rice has been subjected to the process of milling and polishing in which the outer layer (bran) containing fibre has been completely removed. Though flours of yam, cassava, plantain and sorghum were sieved during processing but still contain high fibre content which could be adduced to pore size of the sieve. Diets with a high content of fiber, have a positive effect on health since their consumption has been related to a decreased incidence of several types of diseases as due to its beneficial effects like increasing the volume of fecal bulk, decreasing the time of intestinal transit, lowering cholesterol and glycemic levels, and stimulating the proliferation of the intestinal flora (Dingra et al., 2012; Souilah et al., 2014). The total and reducing sugar content (Table 1) ranged from 5.8 to 25.6 mg/g and 1.2 to 12.1 mg/g, respectively. The sugar content of banana flour was higher (total and reducing sugar) and this could be attributed to glucose release resulting from the activity of endogenous enzymes during processing of the flour The presence of sugar will help improve taste of the food products. The total starch ranged from 222 to 293 mg/g, Plantain recorded the highest starch, the starch was not significantly different (P < 0.05) among banana, yam, sorghum and cassava flours. Amylose and amylopectin ranged from 8.41 to 26% and 74 to 92.4%, respectively. Sorghum and cassava flour recorded the least values for amylose though according to amylose classification in food (Juliano et al., 1981), these samples could be categorise as having intermediated amylose content. The implication of this amylose level is that these foods is expected to be soft and not sticky and will not become hard when cooling. Heating of starch in the presence of water will lead to gelatinisation that makes starch more easily digested, however after cooling amylose tend to recrystallise and form retrograded amylose which is inaccessible to enzymatic hydrolysis. Amylose and amylopectin are important in determining the structure of a carbohydrate food which may have a profound effect on starch digestibility. The mechanism of how an increased amylose/amylopectin ratio affect glycemic response is that linear amylose chain form a compact structure that limit enzyme accessibility and rate of amylosis (Halistrom, 2011). Amylopectin on the other hand with its branched structure is less ordered and therefore more easily digested. www.scholink.org/ojs/index.php/fsns Food Science and Nutrition Studies Vol. 1, No. 2, 2017 66 Published by SCHOLINK INC. The structural characteristics of digestible starch showed that Rapidly Digestible Starch (RDS), Slowly Digestible Starch (SDS) and Resistant Starch (RS) ranged from 31.8 to 55.2%, 8.9 to 40.2% and 7.8 to 37.4%, respectively. Flours of cassava, banana, sorghum and bread were higher in RDS while low value was reported for plantain and noodles. Also the highest value for RS was found in rice (46.5%) followed by noodles and plantain and low values were found in white sorghum and yam flour though yam had high SDS. In foods, RS could corresponds to the physically inaccessible starches entrapped in cellular matrix or are native uncooked granules, the crystallinity of which makes them scarcely susceptible to hydrolysis or retrograded starch (Englist et al., 1999). RS has also been shown to have positive effects on colonic health by increasing faecal bulk and by generating Short Chain Fatty Acids (SCFAs) such as butyric acid, which is the main energy source for colonocytes and may therefore, have a protective role in inflammatory bowel diseases and colon cancer (Hallstrom et al., 2011). Several factors can explain the difference found in the Resistant Starch quantities, some of which are: interaction of starch with different components present in the food system such as proteins, fats; botanical source of starch; and storage conditions (Perera et al., 2010). From the results, it was observed that noodles recorded high value for fibre as well as Resistant Starch this could be from two sources; through enrichment of the ingredient with soluble dietary fibre or through heat processing of starch that lead to formation of retrograded starch which hinders enzymatic hydrolysis of starch (Englist et al., 1992). Table 1. Total Starch, Rapidly Digested Starch (RDS), Slowly Digested Starch (SDS), Resistance Starch (RS), Amylose and Amylopectin Content of Starchy Food (Expressed as% of Total Starch) Sample Moisture Total Starch RDS % SDS % RS % Amylose % Amylopect in % Fiber % Total sugar mg/g Red sugar mg/g Plaintain flour 11.3 ± 0.1 c 239 ± 13 c 31.8 ± 1.0 f 19.2 ± 0.4 f 31.4 ± 0.5 c 17.1 ± 0.3 c 82.9 ± 0.3 c 1.3 ± 0.02 b 10.5 ± 0.5 e 8.45 ± 1.4 b Banana flour 9.8 ± 0.2 d 242 ± 0.9 b 53.7 ± 0.4 b 8.9 ± 0.5 g 37.4 ± 1.2 b 17.7 ± 0.3 c 82.3 ± 0.3 c 1.4 ± 0.05 b 24.6 ± 0.6 ab 12.1 ± 0.6 a Yam Flour 15.3 ± 0.3 a 248 ± 1.0 b 44.3 ± 0.2 d 47.9 ± 1.6 a 7.8 ± 0.1 h 14.5 ± 0.2 d 85.5 ± 0.2 b 3.22 ± 0.8 a 21.1 ± 1.4 b 1.45 ± 0.5 e Red Sorghu 12.7 ± 1.1 b 246 ± 0.9 b 51.6 ± 0.6 bc 33.3 ± 0.2 c 15.1 ± 0.6 f 14.4 ± 0.3 d 85.6 ± 0.3 b 2.29 ± 0.09 a 15.6 ± 0.26 d 2.6 ± 0.07 d white Sorghum 13.1 ± 0.6 b 241 ± 10 c 49.4 ± 0.2 c 40.2 ± 1.5 b 10.4 ± 0.2 gh 8.41 ± 0.7 e 92.4 ± 0.5 a 2.0 ± 0.07 ab 17.1 ± 0.22 c 2.2 ± 0.07 d Cassava flour 12.5 ± 1.0 b 248 ± 2.0 b 55.2 ± 1.0 a 22.6 ± 0.1 e 22.2 ± 0.4 e 12.8 ± 1.1 d 87.8 ± 2.0 b 3.6 ± 0.3 a 10.08 ± 0.05 e 1.83 ± 0.05 e Rice 8.2 ± 0.3 e 222 ± 0.14 c 38.0 ± 0.3 e 16.5 ± 0.2 f 46.5 ± 0.9 a 26.0 ± 0.8 a 74.0 ± 0.6 e 0.48 ± 0.01 c 10.9 ± 0.02 e 1.17 ± 0.13 e Semovita 10.2 ± 1.2 cd 237 ± 8.0 c 43.8 ± 0.3 d 27.9 ± 1.4 d 28.3 ± 1.0 d 17.4 ± 0.2 c 82.6 ± 0.2 c 2.4 ± 0.01 a 25.6 ± 0.03 a 2.71 ± 0.1 d Noodles 12.4 ± 0.8 b 236 ± 5.0 c 30.8 ± 0.8 f 35.4 ± 0.8 c 33.8 ± 0.6 c 19.2 ± 0.6 b 80.8 ± 4.0 d 4.9 ± 0.9 a 5.8 ± 0.62 f 5.3 ± 0.007 c Bread 10.6 ± 0.3 cd 293 ± 5.0 a 49.0 ± 1.0 c 21.0 ± 1.0 ef 30.0 ± 2.0 c 21.0 ± 0.2 b 79.0 ± 0.8 d 1.2 ± 0.02 b 17.8 ± 0.67 c 6.53 ± 0.5 b Note. Mean ± SD—mean and standard deviation of triplicate analysis. Values in the same column with same superscript are not significantly different P ≤ 0.05. 1 Rapidly digestible starch; 2 Slowly digestible starch; 3 Resistance starch. www.scholink.org/ojs/index.php/fsns Food Science and Nutrition Studies Vol. 1, No. 2, 2017 67 Published by SCHOLINK INC. The results of in-vitro digestibility and the kinetic parameter were presented in Figure 1 and Table 2, the results indicated that the equilibrium Concentration (Cα), kinetic constant (K), hydrolysis index (HI) and Glycemic Index (GI) ranged from 34 to 64.9, and 0.02 to 0.07, 56.6 to 104 and 71 to 96.8, respectively. With the exception of yam flour and noodles that recorded low hydrolysis index, all sample had both high hydrolysis and Glycemic Index. Correlation coefficient (Table 3) showed that there is a positive correlation HI and RDS (r = 0.700), GI and RDS (r = 0.701) whereas fibre was negatively correlated with GI (r = -0.624) and HI (r = -0.628). Figure 1. Starch Hydrolysis Curve of Some Starch Foods in Nigeria Table 2. Kinetic Parameters of In-Vitro Starch Hydrolysis of Starchy Foods * white bread—control sample. Mean and standard deviation of triplicate analysis. Cα-equilibrium concentration, K-rate constant, AUC-Area Under Curve, HI-Hydrolysis Index, GI-Glycemic Index. Values within the same column with different superscripts are significantly different at P ≤ 0.05. Sample Cα K AUC HI(%) GI (%) Plaintain flour 44.7 ± 1.0d 0.03 ± 0.001c 6606 ± 12c 73.01 ± 0.9d 79.8 ± 2.1c Banana flour 60.0 ± 3.0b 0.07 ± 0.002a 9442 ± 22a 104 ± 10a 96.8 ± 1.1a Yam Flour 43.7 ± 0.8 d 0.02 ± 0.001d 5911 ± 10g 65.32 ± 0.8e 75.6 ± 1.2d Semovita 55.2 ± 0.7c 0.05 ± 0.001b 8800 ± 2.9d 97.2 ± 0.5c 93.0 ± 2.8b White Sorghum flour 61.9 ± 0.1ab 0.07 ± 0.001a 9014 ± 23c 99.6 ± 1.4b 94.4 ± 3.0ab Red Sorghum flour 64.9 ± 2.0a 0.07 ± 0.004a 9113 ± 25b 100 ± 6.0b 94.8 ± 4.0a Cassava flour 60.0 ± 0.6b 0.07 ± 0.009a 9403 ± 15a 104 ± 0.7a 96.8 ± 1.2a Rice 58.6 ± 0.5c 0.03 ± 0.001c 8603 ± 9.0e 95.1 ± 2.6c 91.9 ± 0.5b Noodles 39.0 ± 0.4e 0.02 ± 0.002d 5123 ± 8.5h 56.6 ± 0.8f 71.0 ± 0.8e Bread* 54.5 ± 0.3c 0.07 ± 0.001a 9048 ± 12h 100 ± 0.0b 94.6 ± 0.3ab www.scholink.org/ojs/index.php/fsns Food Science and Nutrition Studies Vol. 1, No. 2, 2017 68 Published by SCHOLINK INC. The kinetic constant K of amylolysis has been proposed as a reliable index of the inherent susceptibility of flour starches to amylase hydrolysis (Goni et al., 1997; Frei et al., 2003). From the results, the hydrolysis constant of plantain flour, yam flour and noodles is low (k = 0.02), the content of RSD was lower and RS was higher than in bread, this implied that the rate at which they will digest may take a longer time which may not adversely affect the blood sugar. This observation agrees with the report from in-vivo study that yam and unripe plantain had low Glycemic Index (Fasanmade & Anyakuro, 2007; Asinobi et al., 2016). Banana flour unlike plantain recorded high hydrolysis constant (K = 0.07) which is the same as bread, the flour recoded high RDS and sugar content which may result from enzymatic degradation (endogenous enzymes ) of starch that led to ripening. Sorghum flour (red and while cultivars) also recorded high hydrolysis rate which is higher than bread. The hydrolysis constant in cassava flour also did not differ from that of bread, processing of cassava into flour involve grating, soaking and fermentation to make pulp free of cyanide (a toxic compound that is lethal), during fermentation starch is broken down by enzymes an aerobically to sugar which is rapidly released when it is consumed as food. The Glycemic Index is even higher than white bread (reference food) From this study, it was discovered that the Glycemic Index of cassava flour, banana flour, semovita and sorghum flour (red and white cultivars) were higher whereas those of plantain flour, yam flour and noodles were lower compared to bread which was taking as standard high Glycemic Index food. 4. Conclusion The study revealed that starchy foods which are staple diets among Nigerians are high glycemic load foods and that structure of dietary carbohydrate could greatly influenced the Glycemic Index of the foods, therefore, foods like plantain and noodles with low RDS and low hydrolysis constant may be beneficial in management of diabetes whereas sorghum, semovita, cassava and bread which contain high content of rapidly digestible starch should be taken sparingly. Hence, in order to effectively reduce the high incidence of type 2-diabetes, these foods should be complimented with high protein sources. References AOAC. (2000). Official Methods of Analysis, Association of Official Analytical Chemists (AOAC). Washington DC. Asinobi, C., Uzoagba, H., Mba-Anyadioha, A., & Johnkennedy N. (2016). Glycemic Index of some traditional fortified staple meals on the postprandial blood glucose responses of Nigerian undergraduate students: An open-label study. Functional Foods in Health and Disease, 6(7), 414-424. Bainbridge, Z., Tomlins, K., Wellings, K., & Westby, A. (1996). Method of assessing Quality Charactristics of non grain starch staples Part 3, Laboratory Methods, Natural Resources www.scholink.org/ojs/index.php/fsns Food Science and Nutrition Studies Vol. 1, No. 2, 2017 69 Published by SCHOLINK INC. Institute Chatham, UK. Björck, I. M., Granfeldt, Y., Liljeberg, H., Tovar, J., & Asp, N. G. (1994). Food properties affecting the digestion and absorption of carbohydrates. American Journal of Clinical Nutrition, 59, 699S-705S. Brand-Miller, J., Wolever, T. M. S., Foster-Powell, K., & Colagiuri, S. (2003). The new glucose revolution (2 nd ed.). Marlowe & Company, New York, NY. Deepa, G., Singh, V., & Maidu, K. A. (2010). Comparative study on starch digestibility Glycemic Index and resistance starch of pigmented (“Njavara” and “Jyothi”) and a non pigment (“IR 64”) rice varieties. Journal of food science and technology, 47(6), 644-649. https://doi.org/10.1007/s13197-010-0106-1 Dhingra, D., Michael, M., Rajput, H., & Patil, R. T. (2012). Dietary fibre in foods: A review. Journal of food science and technology, 49(3), 255-266. https://doi.org/10.1007/s13197-011-0365-5 Englyst, H., Kingman, S., & Cummings, J. (1992). Classification and measurement of nutritionally important starch fractions. European Journal of Clinical Nutrition, 46, S33-S50. Englyst, K. N., Englyst, H. N., Hudson, G. J., Cole, T. J., & Cummings, J. H. (1999). Rapidly available glucose in foods: An in vitro measurement that reflects the glycemic response. American Journal of Clinical Nutrition, 69, 448-454. Fasanmade, A. A., & Anyakudo, M. C. (2007). Glycemic Indices of Selected Nigerian Flour Meal Products in Male Type 2 Diabetic Subjects. Diabetologia Croatica, 36-42. Frei, M., Siddhuraju, P., & Becker, K. (2003). Studies on the in vitro starch digestibility and Glycemic Index of six different indigenous rice cultivars from the Philippines. Food Chemistry, 83, 395-402. https://doi.org/10.1016/S0308-8146(03)00101-8 Goni, I., Garcia-Alonsa, A., & Saura-Calixto, F. (1997). A starch hydrolysis procedure to estimate Glycemic Index. Nutrition Research, 17, 427-437. https://doi.org/10.1016/S0271-5317(97)00010-9 Grandfeidt, H. N., Veenstra, J., & Hudson, G. J. (1992). Measurement of Rapidly Available Glucose (RAG) in plant foods: A potential in in-vitro predictor of the glycemic responses. British Journal of Nutrition, 46, 649-660. Hallstrom, E., Sestili, E. F., Lafiandra, D., Bjorck, L., & Ostman, E. (2011). A novel wheat variety with elevated content of amylose increases Resistant Starch formation and may beneficially influence glycaemia in healthy subjects. Food and Nutrition Research, 55, 70-74. https://doi.org/10.3402/fnr.v55i0.7074 Han, X. Z., Ao, Z., Janaswamy, S., Jane, J.-L., Chandrasekaran, R., & Hamaker, B. R. (2006). Development of a low glycemic maize starch: Preparation and characterization. Biomacromolecules, 7, 1162-1168. https://doi.org/10.1021/bm050991e Heacock, P. M., Hertzler, S. R., & Wolf, B. (2004). The glycemic, insulinemic, and breath hydrogen responses in humans to a food starch esteriwed by 1-octenyl succinic anhydride. Nutrition Research, 24, 581-692. https://doi.org/10.1016/j.nutres.2003.10.015 www.scholink.org/ojs/index.php/fsns Food Science and Nutrition Studies Vol. 1, No. 2, 2017 70 Published by SCHOLINK INC. Jenkins, D. J. A. et al. (1981). Glycemic Index of foods: A physiological basis for carbohydrate exchange. American Journal of Clinical Nutrition, 34, 362-366. Jenkins, D. J. A. et al. (1984). The relationship between glycemic response, digestibility and factors influencing the dietary habits of diabetics. American Journal of Clinical Nutrition, 40, 1175-1191. Juliano, B. O., Perez C. M., & Blackney, A. B. (1981). International cooperative testingon the amylose content of milled rice. Starch, 33(5), 157-182. https://doi.org/10.1002/star.19810330504 Miller, G. I. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 51, 126-129. https://doi.org/10.1021/ac60147a030 Perera, A., Meda, V., & Tyler, R. T. (2010). Resistant Starch: A review of analytical protocols for determining Resistant Starch and of factors affecting the Resistant Starch content of foods. Food Research International, 43, 1959-1974. https://doi.org/10.1016/j.foodres.2010.06.003 Seal, C. J., Daly, M. E., Thomas, L. C., Bal, W., Birkett, A. M., JeVcoat, R., & Mathers, J. C. (2003). Postprandial carbohydrate metabolism in healthy subjects and those with type 2 diabetes fed starches with slow and rapid hydrolysis rates determined in-vitro. British Journal of Nutrition, 90, 863-864. https://doi.org/10.1079/BJN2003972 Souilah, R., Djabali, D., Belhadi, B., Mokrane, H., Boudries, N., & Nadjemi, B. (2014). In vitro starch digestion in sorghum flour from Algerian cultivars. Food Science and Nutrition, 2(3), 251-259. https://doi.org/10.1002/fsn3.104 Thomas, R., Wan-Nadiah, W. A., & Rajeev, B. (2013). Physiochemical properties, proximate composition, and cooking qualities of locally grown and imported rice varieties marketed in Penang, Malaysia. International Food Research Journal, 20(3), 1345-1351.