id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
fnp-4584	Boluda, Susana; Mokhtari, Karima; Mégarbane, Bruno; Annane, Djillali; Mathon, Bertrand; Cao, Albert; Adam, Clovis; Androuin, Alexandre; Bielle, Franck; Brochier, Guy; Charlotte, Frédéric; Chougar, Lydia; El Hachimi, Khalid Hamid; Eloit, Marc; Haïk, Stéphane; Hervé, Dominique; Kasri, Amal; Leducq, Valentin; Lehéricy, Stéphane; Levavasseur, Etienne; Lobsiger, Christian; Lorin de La Grandmaison, Geoffroy; Malet, Isabelle; Malissin, Isabelle; Marot, Stéphane; Marty, Serge; Pérot, Philippe; Plu, Isabelle; Prigent, Annick; Stimmer, Lev; Potier, Marie-Claude; Marcelin, Anne-Geneviève; Delatour, Benoît; Duyckaerts, Charles; Seilhean, Danielle	Golgi localization of SARS-CoV-2 spike protein and interaction with furin in cerebral COVID-19 microangiopathy: a clue to the central nervous system involvement?	2023		.htm	application/xhtml+xml	9977	571	55	Cell Culture To improve our understanding of the interaction of the virus with endothelial cells and furin, cultures of four cell lines were used: human endothelial cells derived from Induced pluripotent stem cells (iPSC-d-ECs), a human cerebral microvessel endothelial cell line (hCMEC/D3), a Human Umbilical Vein Endothelial Cell line (HUVEC) and a primate kidney cell line (Vero E6 cells). While no viral particle or viral RNA was found in the brain, the SARS-CoV-2 spike protein was detected in the Golgi apparatus of brain endothelial cells where it closely associated with furin, a host protease known to play a key role in virus replication.	cache/fnp-4584.htm	txt/fnp-4584.txt