id	sid	tid	token	lemma	pos
fnp-2749	1	1	optimizing	optimize	VERB
fnp-2749	1	2	filter	filter	NOUN
fnp-2749	1	3	trap	trap	NOUN
fnp-2749	1	4	assay	assay	NOUN
fnp-2749	1	5	for	for	ADP
fnp-2749	1	6	the	the	DET
fnp-2749	1	7	detection	detection	NOUN
fnp-2749	1	8	of	of	ADP
fnp-2749	1	9	aggregated	aggregate	VERB
fnp-2749	1	10	alpha	alpha	NOUN
fnp-2749	1	11	-	-	PUNCT
fnp-2749	1	12	synuclein	synuclein	NOUN
fnp-2749	1	13	in	in	ADP
fnp-2749	1	14	brain	brain	NOUN
fnp-2749	1	15	samples	sample	NOUN
fnp-2749	1	16	feel	feel	VERB
fnp-2749	1	17	free	free	ADJ
fnp-2749	1	18	to	to	PART
fnp-2749	1	19	add	add	VERB
fnp-2749	1	20	comments	comment	NOUN
fnp-2749	1	21	by	by	ADP
fnp-2749	1	22	clicking	click	VERB
fnp-2749	1	23	these	these	DET
fnp-2749	1	24	icons	icon	NOUN
fnp-2749	1	25	on	on	ADP
fnp-2749	1	26	the	the	DET
fnp-2749	1	27	sidebar	sidebar	NOUN
fnp-2749	1	28	free	free	ADJ
fnp-2749	1	29	neuropathology	neuropathology	NOUN
fnp-2749	1	30	1:14	1:14	NUM
fnp-2749	1	31	(	(	PUNCT
fnp-2749	1	32	2020	2020	NUM
fnp-2749	1	33	)	)	PUNCT
fnp-2749	1	34	letter	letter	NOUN
fnp-2749	1	35	optimizing	optimize	VERB
fnp-2749	1	36	filter	filter	NOUN
fnp-2749	1	37	trap	trap	NOUN
fnp-2749	1	38	assay	assay	NOUN
fnp-2749	1	39	for	for	ADP
fnp-2749	1	40	the	the	DET
fnp-2749	1	41	detection	detection	NOUN
fnp-2749	1	42	of	of	ADP
fnp-2749	1	43	aggregated	aggregate	VERB
fnp-2749	1	44	alpha	alpha	NOUN
fnp-2749	1	45	-	-	PUNCT
fnp-2749	1	46	synuclein	synuclein	NOUN
fnp-2749	1	47	in	in	ADP
fnp-2749	1	48	brain	brain	NOUN
fnp-2749	1	49	samples	sample	NOUN
fnp-2749	1	50	thibauld	thibauld	AUX
fnp-2749	1	51	oullier	oullier	VERB
fnp-2749	1	52	1	1	NUM
fnp-2749	1	53	*	*	NUM
fnp-2749	1	54	,	,	PUNCT
fnp-2749	1	55	alice	alice	PROPN
fnp-2749	1	56	prigent	prigent	PROPN
fnp-2749	1	57	1	1	NUM
fnp-2749	1	58	*	*	PROPN
fnp-2749	1	59	,	,	PUNCT
fnp-2749	1	60	guillaume	guillaume	PROPN
fnp-2749	1	61	chapelet	chapelet	NOUN
fnp-2749	1	62	1,2	1,2	NUM
fnp-2749	1	63	,	,	PUNCT
fnp-2749	1	64	michel	michel	PROPN
fnp-2749	1	65	neunlist	neunlist	PROPN
fnp-2749	1	66	1	1	NUM
fnp-2749	1	67	,	,	PUNCT
fnp-2749	1	68	franck	franck	ADJ
fnp-2749	1	69	letournel	letournel	PROPN
fnp-2749	1	70	3	3	NUM
fnp-2749	1	71	,	,	PUNCT
fnp-2749	1	72	pascal	pascal	PROPN
fnp-2749	1	73	derkinderen	derkinderen	NOUN
fnp-2749	1	74	1,4	1,4	NUM
fnp-2749	1	75	1	1	NUM
fnp-2749	1	76	université	université	X
fnp-2749	1	77	de	de	X
fnp-2749	1	78	nantes	nante	NOUN
fnp-2749	1	79	,	,	PUNCT
fnp-2749	1	80	inserm	inserm	NOUN
fnp-2749	1	81	,	,	PUNCT
fnp-2749	1	82	tens	ten	NOUN
fnp-2749	1	83	,	,	PUNCT
fnp-2749	1	84	the	the	DET
fnp-2749	1	85	enteric	enteric	ADJ
fnp-2749	1	86	nervous	nervous	ADJ
fnp-2749	1	87	system	system	NOUN
fnp-2749	1	88	in	in	ADP
fnp-2749	1	89	gut	gut	NOUN
fnp-2749	1	90	and	and	CCONJ
fnp-2749	1	91	brain	brain	NOUN
fnp-2749	1	92	diseases	disease	NOUN
fnp-2749	1	93	,	,	PUNCT
fnp-2749	1	94	imad	imad	NOUN
fnp-2749	1	95	,	,	PUNCT
fnp-2749	1	96	nantes	nante	NOUN
fnp-2749	1	97	,	,	PUNCT
fnp-2749	1	98	france	france	PROPN
fnp-2749	1	99	2	2	NUM
fnp-2749	1	100	chu	chu	PROPN
fnp-2749	1	101	nantes	nante	NOUN
fnp-2749	1	102	,	,	PUNCT
fnp-2749	1	103	clinical	clinical	ADJ
fnp-2749	1	104	gerontology	gerontology	NOUN
fnp-2749	1	105	department	department	PROPN
fnp-2749	1	106	,	,	PUNCT
fnp-2749	1	107	nantes	nante	NOUN
fnp-2749	1	108	,	,	PUNCT
fnp-2749	1	109	france	france	PROPN
fnp-2749	1	110	3	3	NUM
fnp-2749	1	111	chu	chu	PROPN
fnp-2749	1	112	angers	anger	NOUN
fnp-2749	1	113	,	,	PUNCT
fnp-2749	1	114	neurobiology	neurobiology	NOUN
fnp-2749	1	115	and	and	CCONJ
fnp-2749	1	116	neuropathology	neuropathology	NOUN
fnp-2749	1	117	laboratory	laboratory	NOUN
fnp-2749	1	118	,	,	PUNCT
fnp-2749	1	119	angers	anger	VERB
fnp-2749	1	120	,	,	PUNCT
fnp-2749	1	121	france	france	PROPN
fnp-2749	1	122	4	4	NUM
fnp-2749	1	123	chu	chu	PROPN
fnp-2749	1	124	nantes	nante	NOUN
fnp-2749	1	125	,	,	PUNCT
fnp-2749	1	126	department	department	NOUN
fnp-2749	1	127	of	of	ADP
fnp-2749	1	128	neurology	neurology	NOUN
fnp-2749	1	129	,	,	PUNCT
fnp-2749	1	130	nantes	nante	NOUN
fnp-2749	1	131	,	,	PUNCT
fnp-2749	1	132	france	france	PROPN
fnp-2749	1	133	*	*	PUNCT
fnp-2749	1	134	these	these	DET
fnp-2749	1	135	authors	author	NOUN
fnp-2749	1	136	contributed	contribute	VERB
fnp-2749	1	137	equally	equally	ADV
fnp-2749	1	138	to	to	ADP
fnp-2749	1	139	this	this	DET
fnp-2749	1	140	work	work	NOUN
fnp-2749	1	141	.	.	PUNCT
fnp-2749	2	1	corresponding	correspond	VERB
fnp-2749	2	2	author	author	NOUN
fnp-2749	2	3	:	:	PUNCT
fnp-2749	2	4	pascal	pascal	PROPN
fnp-2749	2	5	derkinderen	derkinderen	PROPN
fnp-2749	2	6	·	·	PUNCT
fnp-2749	2	7	inserm	inserm	NOUN
fnp-2749	2	8	u1235	u1235	PROPN
fnp-2749	2	9	nantes	nante	NOUN
fnp-2749	2	10	·	·	PUNCT
fnp-2749	2	11	1	1	NUM
fnp-2749	2	12	rue	rue	X
fnp-2749	2	13	gaston	gaston	PROPN
fnp-2749	2	14	veil	veil	PROPN
fnp-2749	2	15	·	·	PUNCT
fnp-2749	2	16	44035	44035	NUM
fnp-2749	2	17	nantes	nante	VERB
fnp-2749	2	18	·	·	PUNCT
fnp-2749	2	19	france	france	PROPN
fnp-2749	2	20	·	·	PUNCT
fnp-2749	3	1	tel	tel	ADP
fnp-2749	3	2	:	:	PUNCT
fnp-2749	3	3	+33(0)240165202	+33(0)240165202	PROPN
fnp-2749	3	4	·	·	PUNCT
fnp-2749	3	5	fax	fax	NOUN
fnp-2749	3	6	:	:	PUNCT
fnp-2749	3	7	+33(0)240165203	+33(0)240165203	PROPN
fnp-2749	3	8	pascal.derkinderen@chu-nantes.fr	pascal.derkinderen@chu-nantes.fr	PROPN
fnp-2749	3	9	;	;	PUNCT
fnp-2749	3	10	derkinderenp@yahoo.fr	derkinderenp@yahoo.fr	PROPN
fnp-2749	3	11	submitted	submit	VERB
fnp-2749	3	12	:	:	PUNCT
fnp-2749	3	13	04	04	NUM
fnp-2749	3	14	april	april	PROPN
fnp-2749	3	15	2020	2020	NUM
fnp-2749	3	16	accepted	accept	VERB
fnp-2749	3	17	:	:	PUNCT
fnp-2749	3	18	26	26	NUM
fnp-2749	3	19	april	april	PROPN
fnp-2749	3	20	2020	2020	NUM
fnp-2749	3	21	copyedited	copyedit	VERB
fnp-2749	3	22	by	by	ADP
fnp-2749	3	23	:	:	PUNCT
fnp-2749	3	24	christian	christian	PROPN
fnp-2749	3	25	thomas	thomas	PROPN
fnp-2749	3	26	published	publish	VERB
fnp-2749	3	27	:	:	PUNCT
fnp-2749	4	1	28	28	NUM
fnp-2749	4	2	april	april	PROPN
fnp-2749	4	3	2020	2020	NUM
fnp-2749	4	4	https://doi.org/10.17879/freeneuropathology-2020-2749	https://doi.org/10.17879/freeneuropathology-2020-2749	PROPN
fnp-2749	4	5	additional	additional	ADJ
fnp-2749	4	6	resources	resource	NOUN
fnp-2749	4	7	and	and	CCONJ
fnp-2749	4	8	electronic	electronic	ADJ
fnp-2749	4	9	supplementary	supplementary	ADJ
fnp-2749	4	10	material	material	NOUN
fnp-2749	4	11	:	:	PUNCT
fnp-2749	4	12	supplementary	supplementary	ADJ
fnp-2749	4	13	material	material	NOUN
fnp-2749	4	14	keywords	keyword	NOUN
fnp-2749	4	15	:	:	PUNCT
fnp-2749	4	16	alpha	alpha	NOUN
fnp-2749	4	17	-	-	PUNCT
fnp-2749	4	18	synuclein	synuclein	NOUN
fnp-2749	4	19	,	,	PUNCT
fnp-2749	4	20	parkinson	parkinson	NOUN
fnp-2749	4	21	’s	’s	PART
fnp-2749	4	22	disease	disease	NOUN
fnp-2749	4	23	,	,	PUNCT
fnp-2749	4	24	filter	filter	NOUN
fnp-2749	4	25	trap	trap	NOUN
fnp-2749	4	26	,	,	PUNCT
fnp-2749	4	27	dot	dot	NOUN
fnp-2749	4	28	blot	blot	NOUN
fnp-2749	4	29	,	,	PUNCT
fnp-2749	4	30	aggregates	aggregate	NOUN
fnp-2749	4	31	over	over	ADP
fnp-2749	4	32	the	the	DET
fnp-2749	4	33	past	past	ADJ
fnp-2749	4	34	two	two	NUM
fnp-2749	4	35	decades	decade	NOUN
fnp-2749	4	36	,	,	PUNCT
fnp-2749	4	37	the	the	DET
fnp-2749	4	38	progress	progress	NOUN
fnp-2749	4	39	in	in	ADP
fnp-2749	4	40	molecular	molecular	ADJ
fnp-2749	4	41	genetics	genetic	NOUN
fnp-2749	4	42	and	and	CCONJ
fnp-2749	4	43	neuropathology	neuropathology	NOUN
fnp-2749	4	44	enabled	enable	VERB
fnp-2749	4	45	a	a	DET
fnp-2749	4	46	better	well	ADJ
fnp-2749	4	47	understanding	understanding	NOUN
fnp-2749	4	48	of	of	ADP
fnp-2749	4	49	the	the	DET
fnp-2749	4	50	pathogenesis	pathogenesis	NOUN
fnp-2749	4	51	of	of	ADP
fnp-2749	4	52	neurodegenerative	neurodegenerative	ADJ
fnp-2749	4	53	disorders	disorder	NOUN
fnp-2749	4	54	.	.	PUNCT
fnp-2749	5	1	the	the	DET
fnp-2749	5	2	observation	observation	NOUN
fnp-2749	5	3	that	that	SCONJ
fnp-2749	5	4	abnormal	abnormal	ADJ
fnp-2749	5	5	protein	protein	NOUN
fnp-2749	5	6	accumulation	accumulation	NOUN
fnp-2749	5	7	is	be	AUX
fnp-2749	5	8	characteristic	characteristic	ADJ
fnp-2749	5	9	of	of	ADP
fnp-2749	5	10	a	a	DET
fnp-2749	5	11	particular	particular	ADJ
fnp-2749	5	12	disease	disease	NOUN
fnp-2749	5	13	has	have	AUX
fnp-2749	5	14	led	lead	VERB
fnp-2749	5	15	to	to	ADP
fnp-2749	5	16	a	a	DET
fnp-2749	5	17	neuropathological	neuropathological	ADJ
fnp-2749	5	18	classification	classification	NOUN
fnp-2749	5	19	according	accord	VERB
fnp-2749	5	20	to	to	ADP
fnp-2749	5	21	the	the	DET
fnp-2749	5	22	composition	composition	NOUN
fnp-2749	5	23	of	of	ADP
fnp-2749	5	24	the	the	DET
fnp-2749	5	25	abnormal	abnormal	ADJ
fnp-2749	5	26	protein	protein	NOUN
fnp-2749	5	27	aggregates	aggregate	NOUN
fnp-2749	5	28	[	[	X
fnp-2749	5	29	1	1	NUM
fnp-2749	5	30	]	]	PUNCT
fnp-2749	5	31	.	.	PUNCT
fnp-2749	6	1	several	several	ADJ
fnp-2749	6	2	methods	method	NOUN
fnp-2749	6	3	have	have	AUX
fnp-2749	6	4	been	be	AUX
fnp-2749	6	5	developed	develop	VERB
fnp-2749	6	6	for	for	ADP
fnp-2749	6	7	the	the	DET
fnp-2749	6	8	detection	detection	NOUN
fnp-2749	6	9	of	of	ADP
fnp-2749	6	10	these	these	DET
fnp-2749	6	11	insoluble	insoluble	ADJ
fnp-2749	6	12	protein	protein	NOUN
fnp-2749	6	13	aggregates	aggregate	NOUN
fnp-2749	6	14	including	include	VERB
fnp-2749	6	15	,	,	PUNCT
fnp-2749	6	16	for	for	ADP
fnp-2749	6	17	instance	instance	NOUN
fnp-2749	6	18	,	,	PUNCT
fnp-2749	6	19	dye	dye	NOUN
fnp-2749	6	20	binding	bind	VERB
fnp-2749	6	21	assays	assay	NOUN
fnp-2749	6	22	and	and	CCONJ
fnp-2749	6	23	immunohistochemistry	immunohistochemistry	NOUN
fnp-2749	6	24	with	with	ADP
fnp-2749	6	25	proteinase	proteinase	PROPN
fnp-2749	6	26	k	k	PROPN
fnp-2749	6	27	pretreatment	pretreatment	NOUN
fnp-2749	6	28	[	[	X
fnp-2749	6	29	1	1	NUM
fnp-2749	6	30	]	]	PUNCT
fnp-2749	6	31	.	.	PUNCT
fnp-2749	7	1	filter	filter	NOUN
fnp-2749	7	2	retardation	retardation	NOUN
fnp-2749	7	3	assay	assay	NOUN
fnp-2749	7	4	is	be	AUX
fnp-2749	7	5	a	a	DET
fnp-2749	7	6	simple	simple	ADJ
fnp-2749	7	7	and	and	CCONJ
fnp-2749	7	8	rapid	rapid	ADJ
fnp-2749	7	9	method	method	NOUN
fnp-2749	7	10	which	which	PRON
fnp-2749	7	11	detects	detect	VERB
fnp-2749	7	12	protein	protein	NOUN
fnp-2749	7	13	aggregates	aggregate	NOUN
fnp-2749	7	14	formed	form	VERB
fnp-2749	7	15	either	either	CCONJ
fnp-2749	7	16	in	in	ADP
fnp-2749	7	17	vivo	vivo	NOUN
fnp-2749	7	18	or	or	CCONJ
fnp-2749	7	19	in	in	ADP
fnp-2749	7	20	vitro	vitro	X
fnp-2749	7	21	,	,	PUNCT
fnp-2749	7	22	including	include	VERB
fnp-2749	7	23	amyloid	amyloid	NOUN
fnp-2749	7	24	-	-	PUNCT
fnp-2749	7	25	beta	beta	NOUN
fnp-2749	7	26	/	/	SYM
fnp-2749	7	27	tau	tau	PROPN
fnp-2749	7	28	aggregates	aggregate	NOUN
fnp-2749	7	29	from	from	ADP
fnp-2749	7	30	alzheimer	alzheimer	PROPN
fnp-2749	7	31	’s	’s	PART
fnp-2749	7	32	disease	disease	NOUN
fnp-2749	7	33	(	(	PUNCT
fnp-2749	7	34	ad	ad	NOUN
fnp-2749	7	35	)	)	PUNCT
fnp-2749	8	1	[	[	X
fnp-2749	8	2	2–4	2–4	X
fnp-2749	8	3	]	]	X
fnp-2749	8	4	and	and	CCONJ
fnp-2749	8	5	polyglutamine	polyglutamine	NOUN
fnp-2749	8	6	expansion	expansion	NOUN
fnp-2749	8	7	from	from	ADP
fnp-2749	8	8	huntington	huntington	PROPN
fnp-2749	8	9	’s	’s	PART
fnp-2749	8	10	disease	disease	NOUN
fnp-2749	8	11	[	[	X
fnp-2749	8	12	5,6	5,6	NUM
fnp-2749	8	13	]	]	PUNCT
fnp-2749	8	14	.	.	PUNCT
fnp-2749	9	1	in	in	ADP
fnp-2749	9	2	this	this	DET
fnp-2749	9	3	assay	assay	NOUN
fnp-2749	9	4	,	,	PUNCT
fnp-2749	9	5	‘	'	PUNCT
fnp-2749	9	6	lysis	lysis	NOUN
fnp-2749	9	7	buffer’-resistant	buffer’-resistant	NOUN
fnp-2749	9	8	protein	protein	NOUN
fnp-2749	9	9	aggregates	aggregate	NOUN
fnp-2749	9	10	,	,	PUNCT
fnp-2749	9	11	but	but	CCONJ
fnp-2749	9	12	not	not	PART
fnp-2749	9	13	soluble	soluble	ADJ
fnp-2749	9	14	monomeric	monomeric	ADJ
fnp-2749	9	15	species	specie	NOUN
fnp-2749	9	16	,	,	PUNCT
fnp-2749	9	17	are	be	AUX
fnp-2749	9	18	retained	retain	VERB
fnp-2749	9	19	by	by	ADP
fnp-2749	9	20	a	a	DET
fnp-2749	9	21	cellulose	cellulose	NOUN
fnp-2749	9	22	acetate	acetate	NOUN
fnp-2749	9	23	membrane	membrane	NOUN
fnp-2749	9	24	and	and	CCONJ
fnp-2749	9	25	subsequently	subsequently	ADV
fnp-2749	9	26	detected	detect	VERB
fnp-2749	9	27	by	by	ADP
fnp-2749	9	28	immunoblotting	immunoblotte	VERB
fnp-2749	9	29	.	.	PUNCT
fnp-2749	10	1	as	as	ADP
fnp-2749	10	2	loading	loading	NOUN
fnp-2749	10	3	control	control	NOUN
fnp-2749	10	4	,	,	PUNCT
fnp-2749	10	5	a	a	DET
fnp-2749	10	6	‘	'	PUNCT
fnp-2749	10	7	classical	classical	ADJ
fnp-2749	10	8	’	'	PUNCT
fnp-2749	10	9	dot	dot	NOUN
fnp-2749	10	10	blot	blot	NOUN
fnp-2749	10	11	with	with	ADP
fnp-2749	10	12	either	either	CCONJ
fnp-2749	10	13	polyvinylidene	polyvinylidene	NOUN
fnp-2749	10	14	fluoride	fluoride	NOUN
fnp-2749	10	15	(	(	PUNCT
fnp-2749	10	16	pvdf	pvdf	PROPN
fnp-2749	10	17	)	)	PUNCT
fnp-2749	10	18	or	or	CCONJ
fnp-2749	10	19	nitrocellulose	nitrocellulose	NOUN
fnp-2749	10	20	membranes	membrane	NOUN
fnp-2749	10	21	is	be	AUX
fnp-2749	10	22	run	run	VERB
fnp-2749	10	23	in	in	ADP
fnp-2749	10	24	parallel	parallel	NOUN
fnp-2749	10	25	with	with	ADP
fnp-2749	10	26	the	the	DET
fnp-2749	10	27	same	same	ADJ
fnp-2749	10	28	lysates	lysate	NOUN
fnp-2749	10	29	.	.	PUNCT
fnp-2749	11	1	in	in	ADP
fnp-2749	11	2	contrast	contrast	NOUN
fnp-2749	11	3	to	to	ADP
fnp-2749	11	4	polyglutamine	polyglutamine	NOUN
fnp-2749	11	5	expansion	expansion	NOUN
fnp-2749	11	6	,	,	PUNCT
fnp-2749	11	7	tau	tau	PROPN
fnp-2749	11	8	and	and	CCONJ
fnp-2749	11	9	beta	beta	NOUN
fnp-2749	11	10	-	-	PUNCT
fnp-2749	11	11	amyloid	amyloid	NOUN
fnp-2749	11	12	,	,	PUNCT
fnp-2749	11	13	there	there	PRON
fnp-2749	11	14	are	be	VERB
fnp-2749	11	15	only	only	ADV
fnp-2749	11	16	few	few	ADJ
fnp-2749	11	17	studies	study	NOUN
fnp-2749	11	18	on	on	ADP
fnp-2749	11	19	using	use	VERB
fnp-2749	11	20	filter	filter	NOUN
fnp-2749	11	21	retardation	retardation	NOUN
fnp-2749	11	22	assays	assay	NOUN
fnp-2749	11	23	for	for	ADP
fnp-2749	11	24	the	the	DET
fnp-2749	11	25	detection	detection	NOUN
fnp-2749	11	26	of	of	ADP
fnp-2749	11	27	alpha	alpha	NOUN
fnp-2749	11	28	-	-	PUNCT
fnp-2749	11	29	synuclein	synuclein	NOUN
fnp-2749	11	30	aggregates	aggregate	NOUN
fnp-2749	11	31	,	,	PUNCT
fnp-2749	11	32	which	which	PRON
fnp-2749	11	33	are	be	AUX
fnp-2749	11	34	characteristic	characteristic	ADJ
fnp-2749	11	35	of	of	ADP
fnp-2749	11	36	parkinson	parkinson	NOUN
fnp-2749	11	37	’s	’s	PART
fnp-2749	11	38	disease	disease	NOUN
fnp-2749	11	39	(	(	PUNCT
fnp-2749	11	40	pd	pd	NOUN
fnp-2749	11	41	)	)	PUNCT
fnp-2749	11	42	and	and	CCONJ
fnp-2749	11	43	dementia	dementia	NOUN
fnp-2749	11	44	with	with	ADP
fnp-2749	11	45	lewy	lewy	ADJ
fnp-2749	11	46	bodies	body	NOUN
fnp-2749	11	47	(	(	PUNCT
fnp-2749	11	48	dlb	dlb	NOUN
fnp-2749	11	49	)	)	PUNCT
fnp-2749	12	1	[	[	X
fnp-2749	12	2	7	7	NUM
fnp-2749	12	3	]	]	PUNCT
fnp-2749	12	4	.	.	PUNCT
fnp-2749	13	1	in	in	ADP
fnp-2749	13	2	addition	addition	NOUN
fnp-2749	13	3	,	,	PUNCT
fnp-2749	13	4	a	a	DET
fnp-2749	13	5	full	full	ADJ
fnp-2749	13	6	characterization	characterization	NOUN
fnp-2749	13	7	of	of	ADP
fnp-2749	13	8	any	any	DET
fnp-2749	13	9	filter	filter	NOUN
fnp-2749	13	10	-	-	PUNCT
fnp-2749	13	11	based	base	VERB
fnp-2749	13	12	assay	assay	NOUN
fnp-2749	13	13	for	for	ADP
fnp-2749	13	14	alpha	alpha	NOUN
fnp-2749	13	15	-	-	PUNCT
fnp-2749	13	16	synuclein	synuclein	NOUN
fnp-2749	13	17	,	,	PUNCT
fnp-2749	13	18	including	include	VERB
fnp-2749	13	19	the	the	DET
fnp-2749	13	20	effect	effect	NOUN
fnp-2749	13	21	of	of	ADP
fnp-2749	13	22	membrane	membrane	NOUN
fnp-2749	13	23	composition	composition	NOUN
fnp-2749	13	24	,	,	PUNCT
fnp-2749	13	25	lysis	lysis	NOUN
fnp-2749	13	26	procedure	procedure	NOUN
fnp-2749	13	27	and	and	CCONJ
fnp-2749	13	28	antibodies	antibody	NOUN
fnp-2749	13	29	has	have	AUX
fnp-2749	13	30	not	not	PART
fnp-2749	13	31	yet	yet	ADV
fnp-2749	13	32	been	be	AUX
fnp-2749	13	33	reported	report	VERB
fnp-2749	13	34	.	.	PUNCT
fnp-2749	14	1	we	we	PRON
fnp-2749	14	2	therefore	therefore	ADV
fnp-2749	14	3	set	set	VERB
fnp-2749	14	4	out	out	ADP
fnp-2749	14	5	the	the	DET
fnp-2749	14	6	current	current	ADJ
fnp-2749	14	7	study	study	NOUN
fnp-2749	14	8	in	in	ADP
fnp-2749	14	9	which	which	PRON
fnp-2749	14	10	we	we	PRON
fnp-2749	14	11	characterize	characterize	VERB
fnp-2749	14	12	a	a	DET
fnp-2749	14	13	vacuum	vacuum	NOUN
fnp-2749	14	14	-	-	PUNCT
fnp-2749	14	15	based	base	VERB
fnp-2749	14	16	96	96	NUM
fnp-2749	14	17	-	-	PUNCT
fnp-2749	14	18	well	well	NOUN
fnp-2749	14	19	format	format	NOUN
fnp-2749	14	20	filter	filter	NOUN
fnp-2749	14	21	assay	assay	VERB
fnp-2749	14	22	for	for	ADP
fnp-2749	14	23	the	the	DET
fnp-2749	14	24	detection	detection	NOUN
fnp-2749	14	25	of	of	ADP
fnp-2749	14	26	aggregated	aggregate	VERB
fnp-2749	14	27	alpha	alpha	NOUN
fnp-2749	14	28	-	-	PUNCT
fnp-2749	14	29	synuclein	synuclein	NOUN
fnp-2749	14	30	in	in	ADP
fnp-2749	14	31	human	human	ADJ
fnp-2749	14	32	brain	brain	NOUN
fnp-2749	14	33	specimens	specimen	NOUN
fnp-2749	14	34	.	.	PUNCT
fnp-2749	15	1	frozen	frozen	ADJ
fnp-2749	15	2	brain	brain	NOUN
fnp-2749	15	3	samples	sample	NOUN
fnp-2749	15	4	from	from	ADP
fnp-2749	15	5	neuropathologically	neuropathologically	ADV
fnp-2749	15	6	confirmed	confirm	VERB
fnp-2749	15	7	cases	case	NOUN
fnp-2749	15	8	of	of	ADP
fnp-2749	15	9	dlb	dlb	NOUN
fnp-2749	15	10	,	,	PUNCT
fnp-2749	15	11	progressive	progressive	ADJ
fnp-2749	15	12	supranuclear	supranuclear	ADJ
fnp-2749	15	13	palsy	palsy	ADJ
fnp-2749	15	14	(	(	PUNCT
fnp-2749	15	15	psp	psp	NOUN
fnp-2749	15	16	)	)	PUNCT
fnp-2749	15	17	and	and	CCONJ
fnp-2749	15	18	ad	ad	NOUN
fnp-2749	15	19	were	be	AUX
fnp-2749	15	20	obtained	obtain	VERB
fnp-2749	15	21	from	from	ADP
fnp-2749	15	22	the	the	DET
fnp-2749	15	23	neuropathology	neuropathology	NOUN
fnp-2749	15	24	department	department	NOUN
fnp-2749	15	25	of	of	ADP
fnp-2749	15	26	angers	anger	NOUN
fnp-2749	15	27	(	(	PUNCT
fnp-2749	15	28	dr	dr	PROPN
fnp-2749	15	29	franck	franck	PROPN
fnp-2749	15	30	letournel	letournel	PROPN
fnp-2749	15	31	)	)	PUNCT
fnp-2749	15	32	.	.	PUNCT
fnp-2749	16	1	brain	brain	NOUN
fnp-2749	16	2	samples	sample	NOUN
fnp-2749	16	3	were	be	AUX
fnp-2749	16	4	lysed	lyse	VERB
fnp-2749	16	5	with	with	ADP
fnp-2749	16	6	the	the	DET
fnp-2749	16	7	“	"	PUNCT
fnp-2749	16	8	precellys	precellys	NOUN
fnp-2749	16	9	24	24	NUM
fnp-2749	16	10	™	™	ADJ
fnp-2749	16	11	”	"	PUNCT
fnp-2749	16	12	tissue	tissue	NOUN
fnp-2749	16	13	homogenizer	homogenizer	NOUN
fnp-2749	16	14	(	(	PUNCT
fnp-2749	16	15	bertin	bertin	PROPN
fnp-2749	16	16	technologies	technology	NOUN
fnp-2749	16	17	,	,	PUNCT
fnp-2749	16	18	cat	cat	NOUN
fnp-2749	16	19	#	#	NOUN
fnp-2749	16	20	p000669	p000669	NUM
fnp-2749	16	21	-	-	PUNCT
fnp-2749	16	22	pr240	pr240	NOUN
fnp-2749	16	23	-	-	PUNCT
fnp-2749	16	24	a	a	PRON
fnp-2749	16	25	,	,	PUNCT
fnp-2749	16	26	montigny	montigny	NOUN
fnp-2749	16	27	-	-	PUNCT
fnp-2749	16	28	le	le	NOUN
fnp-2749	16	29	-	-	PUNCT
fnp-2749	16	30	bretonneux	bretonneux	PROPN
fnp-2749	16	31	,	,	PUNCT
fnp-2749	16	32	france	france	PROPN
fnp-2749	16	33	)	)	PUNCT
fnp-2749	16	34	followed	follow	VERB
fnp-2749	16	35	by	by	ADP
fnp-2749	16	36	a	a	DET
fnp-2749	16	37	brief	brief	ADJ
fnp-2749	16	38	sonication	sonication	NOUN
fnp-2749	16	39	(	(	PUNCT
fnp-2749	16	40	one	one	NUM
fnp-2749	16	41	minute	minute	NOUN
fnp-2749	16	42	,	,	PUNCT
fnp-2749	16	43	5	5	NUM
fnp-2749	16	44	seconds	second	NOUN
fnp-2749	16	45	on	on	ADP
fnp-2749	16	46	,	,	PUNCT
fnp-2749	16	47	5	5	NUM
fnp-2749	16	48	seconds	second	NOUN
fnp-2749	16	49	off	off	ADV
fnp-2749	16	50	,	,	PUNCT
fnp-2749	16	51	amplitude	amplitude	NOUN
fnp-2749	16	52	70	70	NUM
fnp-2749	16	53	%	%	NOUN
fnp-2749	16	54	;	;	PUNCT
fnp-2749	16	55	vcx130	vcx130	NOUN
fnp-2749	16	56	ultrasonic	ultrasonic	ADJ
fnp-2749	16	57	processor	processor	NOUN
fnp-2749	16	58	with	with	ADP
fnp-2749	16	59	a	a	DET
fnp-2749	16	60	6	6	NUM
fnp-2749	16	61	mm	mm	NOUN
fnp-2749	16	62	probe	probe	NOUN
fnp-2749	16	63	,	,	PUNCT
fnp-2749	16	64	sonics	sonics	PROPN
fnp-2749	16	65	and	and	CCONJ
fnp-2749	16	66	materials	material	NOUN
fnp-2749	16	67	,	,	PUNCT
fnp-2749	16	68	newton	newton	PROPN
fnp-2749	16	69	,	,	PUNCT
fnp-2749	16	70	ct	ct	PROPN
fnp-2749	16	71	,	,	PUNCT
fnp-2749	16	72	usa	usa	PROPN
fnp-2749	16	73	)	)	PUNCT
fnp-2749	16	74	either	either	CCONJ
fnp-2749	16	75	in	in	ADP
fnp-2749	16	76	(	(	PUNCT
fnp-2749	16	77	i	i	NOUN
fnp-2749	16	78	)	)	PUNCT
fnp-2749	16	79	radioimmunoprecipitation	radioimmunoprecipitation	NOUN
fnp-2749	16	80	assay	assay	PROPN
fnp-2749	16	81	(	(	PUNCT
fnp-2749	16	82	ripa	ripa	NOUN
fnp-2749	16	83	)	)	PUNCT
fnp-2749	16	84	buffer	buffer	NOUN
fnp-2749	16	85	(	(	PUNCT
fnp-2749	16	86	merck	merck	PROPN
fnp-2749	16	87	millipore	millipore	PROPN
fnp-2749	16	88	,	,	PUNCT
fnp-2749	16	89	cat	cat	NOUN
fnp-2749	16	90	#	#	NOUN
fnp-2749	16	91	20	20	NUM
fnp-2749	16	92	-	-	SYM
fnp-2749	16	93	188	188	NUM
fnp-2749	16	94	,	,	PUNCT
fnp-2749	16	95	molsheim	molsheim	PROPN
fnp-2749	16	96	,	,	PUNCT
fnp-2749	16	97	france	france	PROPN
fnp-2749	16	98	)	)	PUNCT
fnp-2749	16	99	containing	contain	VERB
fnp-2749	16	100	2	2	NUM
fnp-2749	16	101	mm	mm	NOUN
fnp-2749	16	102	orthovanadate	orthovanadate	NOUN
fnp-2749	16	103	(	(	PUNCT
fnp-2749	16	104	sigma	sigma	PROPN
fnp-2749	16	105	,	,	PUNCT
fnp-2749	16	106	cat	cat	NOUN
fnp-2749	16	107	#	#	NOUN
fnp-2749	16	108	s6505	s6505	PROPN
fnp-2749	16	109	,	,	PUNCT
fnp-2749	16	110	saint	saint	PROPN
fnp-2749	16	111	quentin	quentin	PROPN
fnp-2749	16	112	fallavier	fallavier	PROPN
fnp-2749	16	113	,	,	PUNCT
fnp-2749	16	114	france	france	PROPN
fnp-2749	16	115	)	)	PUNCT
fnp-2749	16	116	,	,	PUNCT
fnp-2749	16	117	1	1	NUM
fnp-2749	16	118	%	%	NOUN
fnp-2749	16	119	(	(	PUNCT
fnp-2749	16	120	v	v	NOUN
fnp-2749	16	121	/	/	SYM
fnp-2749	16	122	v	v	NOUN
fnp-2749	16	123	)	)	PUNCT
fnp-2749	16	124	phosphatase	phosphatase	NOUN
fnp-2749	16	125	inhibitor	inhibitor	NOUN
fnp-2749	16	126	cocktail	cocktail	NOUN
fnp-2749	16	127	iii	iii	NOUN
fnp-2749	16	128	(	(	PUNCT
fnp-2749	16	129	sigma	sigma	PROPN
fnp-2749	16	130	,	,	PUNCT
fnp-2749	16	131	cat	cat	NOUN
fnp-2749	16	132	#	#	NOUN
fnp-2749	16	133	p0044	p0044	PROPN
fnp-2749	16	134	)	)	PUNCT
fnp-2749	16	135	and	and	CCONJ
fnp-2749	16	136	a	a	DET
fnp-2749	16	137	protease	protease	NOUN
fnp-2749	16	138	inhibitors	inhibitor	NOUN
fnp-2749	16	139	cocktail	cocktail	NOUN
fnp-2749	16	140	(	(	PUNCT
fnp-2749	16	141	roche	roche	NOUN
fnp-2749	16	142	,	,	PUNCT
fnp-2749	16	143	cat	cat	NOUN
fnp-2749	16	144	#	#	NOUN
fnp-2749	16	145	14424700	14424700	NUM
fnp-2749	16	146	,	,	PUNCT
fnp-2749	16	147	meylan	meylan	NOUN
fnp-2749	16	148	,	,	PUNCT
fnp-2749	16	149	france	france	PROPN
fnp-2749	16	150	)	)	PUNCT
fnp-2749	16	151	at	at	ADP
fnp-2749	16	152	4	4	NUM
fnp-2749	16	153	°	°	NOUN
fnp-2749	16	154	c	c	NOUN
fnp-2749	16	155	;	;	PUNCT
fnp-2749	16	156	(	(	PUNCT
fnp-2749	16	157	ii	ii	NOUN
fnp-2749	16	158	)	)	PUNCT
fnp-2749	16	159	‘	'	PUNCT
fnp-2749	16	160	sodium	sodium	NOUN
fnp-2749	16	161	dodecyl	dodecyl	NOUN
fnp-2749	16	162	sulfate	sulfate	NOUN
fnp-2749	16	163	(	(	PUNCT
fnp-2749	16	164	sds	sds	NOUN
fnp-2749	16	165	)	)	PUNCT
fnp-2749	16	166	buffer	buffer	NOUN
fnp-2749	16	167	’	'	PUNCT
fnp-2749	16	168	containing	contain	VERB
fnp-2749	16	169	1	1	NUM
fnp-2749	16	170	%	%	NOUN
fnp-2749	16	171	(	(	PUNCT
fnp-2749	16	172	w	w	NOUN
fnp-2749	16	173	/	/	SYM
fnp-2749	16	174	v	v	NOUN
fnp-2749	16	175	)	)	PUNCT
fnp-2749	16	176	sds	sds	NOUN
fnp-2749	16	177	,	,	PUNCT
fnp-2749	16	178	2	2	NUM
fnp-2749	16	179	mm	mm	NOUN
fnp-2749	16	180	orthovanadate	orthovanadate	NOUN
fnp-2749	16	181	(	(	PUNCT
fnp-2749	16	182	sigma	sigma	PROPN
fnp-2749	16	183	,	,	PUNCT
fnp-2749	16	184	cat	cat	NOUN
fnp-2749	16	185	#	#	NOUN
fnp-2749	16	186	s6505	s6505	PROPN
fnp-2749	16	187	)	)	PUNCT
fnp-2749	16	188	and	and	CCONJ
fnp-2749	16	189	1	1	NUM
fnp-2749	16	190	%	%	NOUN
fnp-2749	16	191	(	(	PUNCT
fnp-2749	16	192	v	v	NOUN
fnp-2749	16	193	/	/	SYM
fnp-2749	16	194	v	v	NOUN
fnp-2749	16	195	)	)	PUNCT
fnp-2749	16	196	phosphatase	phosphatase	NOUN
fnp-2749	16	197	inhibitor	inhibitor	NOUN
fnp-2749	16	198	cocktail	cocktail	NOUN
fnp-2749	16	199	iii	iii	NOUN
fnp-2749	16	200	(	(	PUNCT
fnp-2749	16	201	sigma	sigma	PROPN
fnp-2749	16	202	,	,	PUNCT
fnp-2749	16	203	cat	cat	NOUN
fnp-2749	16	204	#	#	NOUN
fnp-2749	16	205	p0044	p0044	PROPN
fnp-2749	16	206	)	)	PUNCT
fnp-2749	16	207	;	;	PUNCT
fnp-2749	16	208	(	(	PUNCT
fnp-2749	16	209	iii	iii	NOUN
fnp-2749	16	210	)	)	PUNCT
fnp-2749	16	211	‘	'	PUNCT
fnp-2749	16	212	urea	urea	NOUN
fnp-2749	16	213	buffer	buffer	NOUN
fnp-2749	16	214	’	'	PUNCT
fnp-2749	16	215	containing	contain	VERB
fnp-2749	16	216	7	7	NUM
fnp-2749	16	217	m	m	NOUN
fnp-2749	16	218	urea	urea	NOUN
fnp-2749	16	219	(	(	PUNCT
fnp-2749	16	220	sigma	sigma	PROPN
fnp-2749	16	221	,	,	PUNCT
fnp-2749	16	222	cat	cat	NOUN
fnp-2749	16	223	#	#	NOUN
fnp-2749	16	224	t7875	t7875	NUM
fnp-2749	16	225	)	)	PUNCT
fnp-2749	16	226	,	,	PUNCT
fnp-2749	16	227	2	2	NUM
fnp-2749	16	228	m	m	NOUN
fnp-2749	16	229	thiourea	thiourea	NOUN
fnp-2749	16	230	(	(	PUNCT
fnp-2749	16	231	sigma	sigma	PROPN
fnp-2749	16	232	,	,	PUNCT
fnp-2749	16	233	cat	cat	NOUN
fnp-2749	16	234	#	#	NOUN
fnp-2749	16	235	l3771	l3771	NOUN
fnp-2749	16	236	)	)	PUNCT
fnp-2749	16	237	,	,	PUNCT
fnp-2749	16	238	4	4	NUM
fnp-2749	16	239	%	%	NOUN
fnp-2749	16	240	(	(	PUNCT
fnp-2749	16	241	v	v	NOUN
fnp-2749	16	242	/	/	SYM
fnp-2749	16	243	v	v	NOUN
fnp-2749	16	244	)	)	PUNCT
fnp-2749	16	245	chaps	chap	NOUN
fnp-2749	16	246	(	(	PUNCT
fnp-2749	16	247	sigma	sigma	PROPN
fnp-2749	16	248	,	,	PUNCT
fnp-2749	16	249	cat	cat	NOUN
fnp-2749	16	250	#	#	NOUN
fnp-2749	16	251	c3023	c3023	PROPN
fnp-2749	16	252	)	)	PUNCT
fnp-2749	16	253	,	,	PUNCT
fnp-2749	16	254	2	2	NUM
fnp-2749	16	255	mm	mm	NOUN
fnp-2749	16	256	orthovanadate	orthovanadate	NOUN
fnp-2749	16	257	(	(	PUNCT
fnp-2749	16	258	sigma	sigma	PROPN
fnp-2749	16	259	,	,	PUNCT
fnp-2749	16	260	cat	cat	NOUN
fnp-2749	16	261	#	#	NOUN
fnp-2749	16	262	s6505	s6505	PROPN
fnp-2749	16	263	)	)	PUNCT
fnp-2749	16	264	,	,	PUNCT
fnp-2749	16	265	1	1	NUM
fnp-2749	16	266	%	%	NOUN
fnp-2749	16	267	(	(	PUNCT
fnp-2749	16	268	v	v	NOUN
fnp-2749	16	269	/	/	SYM
fnp-2749	16	270	v	v	NOUN
fnp-2749	16	271	)	)	PUNCT
fnp-2749	16	272	phosphatase	phosphatase	NOUN
fnp-2749	16	273	inhibitor	inhibitor	NOUN
fnp-2749	16	274	cocktail	cocktail	NOUN
fnp-2749	16	275	iii	iii	NOUN
fnp-2749	16	276	(	(	PUNCT
fnp-2749	16	277	sigma	sigma	PROPN
fnp-2749	16	278	,	,	PUNCT
fnp-2749	16	279	cat	cat	NOUN
fnp-2749	16	280	#	#	NOUN
fnp-2749	16	281	p0044	p0044	PROPN
fnp-2749	16	282	)	)	PUNCT
fnp-2749	16	283	and	and	CCONJ
fnp-2749	16	284	a	a	DET
fnp-2749	16	285	protease	protease	NOUN
fnp-2749	16	286	inhibitors	inhibitor	NOUN
fnp-2749	16	287	cocktail	cocktail	NOUN
fnp-2749	16	288	(	(	PUNCT
fnp-2749	16	289	roche	roche	NOUN
fnp-2749	16	290	,	,	PUNCT
fnp-2749	16	291	cat	cat	NOUN
fnp-2749	16	292	#	#	NOUN
fnp-2749	16	293	14424700	14424700	NUM
fnp-2749	16	294	)	)	PUNCT
fnp-2749	16	295	.	.	PUNCT
fnp-2749	17	1	lysates	lysate	NOUN
fnp-2749	17	2	were	be	AUX
fnp-2749	17	3	centrifuged	centrifuge	VERB
fnp-2749	17	4	at	at	ADP
fnp-2749	17	5	10,000	10,000	NUM
fnp-2749	17	6	x	x	SYM
fnp-2749	17	7	g	g	NOUN
fnp-2749	17	8	for	for	ADP
fnp-2749	17	9	5	5	NUM
fnp-2749	17	10	minutes	minute	NOUN
fnp-2749	17	11	;	;	PUNCT
fnp-2749	17	12	supernatants	supernatant	NOUN
fnp-2749	17	13	were	be	AUX
fnp-2749	17	14	recovered	recover	VERB
fnp-2749	17	15	and	and	CCONJ
fnp-2749	17	16	protein	protein	NOUN
fnp-2749	17	17	concentration	concentration	NOUN
fnp-2749	17	18	determined	determine	VERB
fnp-2749	17	19	using	use	VERB
fnp-2749	17	20	the	the	DET
fnp-2749	17	21	pierce	pierce	PROPN
fnp-2749	17	22	bca	bca	PROPN
fnp-2749	17	23	protein	protein	NOUN
fnp-2749	17	24	assay	assay	NOUN
fnp-2749	17	25	kit	kit	NOUN
fnp-2749	17	26	(	(	PUNCT
fnp-2749	17	27	thermofisher	thermofisher	PRON
fnp-2749	17	28	scientific	scientific	ADJ
fnp-2749	17	29	,	,	PUNCT
fnp-2749	17	30	cat	cat	NOUN
fnp-2749	17	31	#	#	NOUN
fnp-2749	17	32	23227	23227	NUM
fnp-2749	17	33	,	,	PUNCT
fnp-2749	17	34	saint	saint	PROPN
fnp-2749	17	35	herblain	herblain	PROPN
fnp-2749	17	36	,	,	PUNCT
fnp-2749	17	37	france	france	PROPN
fnp-2749	17	38	)	)	PUNCT
fnp-2749	17	39	along	along	ADP
fnp-2749	17	40	with	with	ADP
fnp-2749	17	41	the	the	DET
fnp-2749	17	42	varioskan	varioskan	ADJ
fnp-2749	17	43	™	™	ADJ
fnp-2749	17	44	multimode	multimode	NOUN
fnp-2749	17	45	microplate	microplate	NOUN
fnp-2749	17	46	reader	reader	NOUN
fnp-2749	17	47	(	(	PUNCT
fnp-2749	17	48	thermofisher	thermofisher	ADJ
fnp-2749	17	49	scientific	scientific	ADJ
fnp-2749	17	50	,	,	PUNCT
fnp-2749	17	51	cat	cat	NOUN
fnp-2749	17	52	#	#	NOUN
fnp-2749	17	53	vl0l00d0	vl0l00d0	NOUN
fnp-2749	17	54	)	)	PUNCT
fnp-2749	17	55	;	;	PUNCT
fnp-2749	17	56	samples	sample	NOUN
fnp-2749	17	57	lysed	lyse	VERB
fnp-2749	17	58	in	in	ADP
fnp-2749	17	59	urea	urea	NOUN
fnp-2749	17	60	buffer	buffer	NOUN
fnp-2749	17	61	were	be	AUX
fnp-2749	17	62	diluted	dilute	VERB
fnp-2749	17	63	5	5	NUM
fnp-2749	17	64	times	time	NOUN
fnp-2749	17	65	for	for	ADP
fnp-2749	17	66	compatibility	compatibility	NOUN
fnp-2749	17	67	with	with	ADP
fnp-2749	17	68	the	the	DET
fnp-2749	17	69	bca	bca	PROPN
fnp-2749	17	70	assay	assay	PROPN
fnp-2749	17	71	(	(	PUNCT
fnp-2749	17	72	https://assets.thermofisher.com/tfs-assets/lsg/application-notes/tr0068-protein-assay-compatibility.pdf	https://assets.thermofisher.com/tfs-assets/lsg/application-notes/tr0068-protein-assay-compatibility.pdf	PROPN
fnp-2749	17	73	)	)	PUNCT
fnp-2749	17	74	.	.	PUNCT
fnp-2749	18	1	twenty	twenty	NUM
fnp-2749	18	2	µg	µg	NOUN
fnp-2749	18	3	of	of	ADP
fnp-2749	18	4	these	these	DET
fnp-2749	18	5	supernatants	supernatant	NOUN
fnp-2749	18	6	were	be	AUX
fnp-2749	18	7	subjected	subject	VERB
fnp-2749	18	8	to	to	ADP
fnp-2749	18	9	vacuum	vacuum	VERB
fnp-2749	18	10	filtration	filtration	NOUN
fnp-2749	18	11	through	through	ADP
fnp-2749	18	12	a	a	DET
fnp-2749	18	13	96	96	NUM
fnp-2749	18	14	-	-	PUNCT
fnp-2749	18	15	well	well	NOUN
fnp-2749	18	16	dot	dot	NOUN
fnp-2749	18	17	blot	blot	NOUN
fnp-2749	18	18	apparatus	apparatus	NOUN
fnp-2749	18	19	(	(	PUNCT
fnp-2749	18	20	bio	bio	NOUN
fnp-2749	18	21	-	-	PROPN
fnp-2749	18	22	rad	rad	ADJ
fnp-2749	18	23	,	,	PUNCT
fnp-2749	18	24	cat	cat	NOUN
fnp-2749	18	25	#	#	NOUN
fnp-2749	18	26	1706545	1706545	NUM
fnp-2749	18	27	,	,	PUNCT
fnp-2749	18	28	marnes	marne	NOUN
fnp-2749	18	29	-	-	PUNCT
fnp-2749	18	30	la	la	NOUN
fnp-2749	18	31	-	-	PUNCT
fnp-2749	18	32	coquette	coquette	NOUN
fnp-2749	18	33	,	,	PUNCT
fnp-2749	18	34	france	france	PROPN
fnp-2749	18	35	)	)	PUNCT
fnp-2749	18	36	with	with	ADP
fnp-2749	18	37	0.45	0.45	NUM
fnp-2749	18	38	µm	µm	ADP
fnp-2749	18	39	pore	pore	ADJ
fnp-2749	18	40	size	size	NOUN
fnp-2749	18	41	cellulose	cellulose	NOUN
fnp-2749	18	42	acetate	acetate	NOUN
fnp-2749	18	43	(	(	PUNCT
fnp-2749	18	44	sterlitech	sterlitech	NOUN
fnp-2749	18	45	,	,	PUNCT
fnp-2749	18	46	cat	cat	NOUN
fnp-2749	18	47	#	#	NOUN
fnp-2749	18	48	ca0453001	ca0453001	NOUN
fnp-2749	18	49	,	,	PUNCT
fnp-2749	18	50	kent	kent	PROPN
fnp-2749	18	51	,	,	PUNCT
fnp-2749	18	52	usa	usa	PROPN
fnp-2749	18	53	)	)	PUNCT
fnp-2749	18	54	,	,	PUNCT
fnp-2749	18	55	nitrocellulose	nitrocellulose	NOUN
fnp-2749	18	56	(	(	PUNCT
fnp-2749	18	57	thermofisher	thermofisher	PRON
fnp-2749	18	58	scientific	scientific	ADJ
fnp-2749	18	59	,	,	PUNCT
fnp-2749	18	60	cat	cat	NOUN
fnp-2749	18	61	#	#	NOUN
fnp-2749	18	62	77010	77010	NUM
fnp-2749	18	63	)	)	PUNCT
fnp-2749	18	64	or	or	CCONJ
fnp-2749	18	65	pvdf	pvdf	PROPN
fnp-2749	18	66	membranes	membrane	NOUN
fnp-2749	18	67	(	(	PUNCT
fnp-2749	18	68	thermofisher	thermofisher	PRON
fnp-2749	18	69	scientific	scientific	ADJ
fnp-2749	18	70	,	,	PUNCT
fnp-2749	18	71	cat	cat	NOUN
fnp-2749	18	72	#	#	NOUN
fnp-2749	18	73	88518	88518	NUM
fnp-2749	18	74	)	)	PUNCT
fnp-2749	18	75	.	.	PUNCT
fnp-2749	19	1	the	the	DET
fnp-2749	19	2	resultant	resultant	NOUN
fnp-2749	19	3	membranes	membrane	NOUN
fnp-2749	19	4	were	be	AUX
fnp-2749	19	5	blocked	block	VERB
fnp-2749	19	6	for	for	ADP
fnp-2749	19	7	one	one	NUM
fnp-2749	19	8	hour	hour	NOUN
fnp-2749	19	9	at	at	ADP
fnp-2749	19	10	room	room	NOUN
fnp-2749	19	11	temperature	temperature	NOUN
fnp-2749	19	12	in	in	ADP
fnp-2749	19	13	tris	tris	ADJ
fnp-2749	19	14	-	-	PUNCT
fnp-2749	19	15	buffered	buffer	VERB
fnp-2749	19	16	saline	saline	NOUN
fnp-2749	19	17	(	(	PUNCT
fnp-2749	19	18	tbs	tbs	NOUN
fnp-2749	19	19	,	,	PUNCT
fnp-2749	19	20	sigma	sigma	NOUN
fnp-2749	19	21	,	,	PUNCT
fnp-2749	19	22	cat	cat	NOUN
fnp-2749	19	23	#	#	NOUN
fnp-2749	19	24	t5912	t5912	NUM
fnp-2749	19	25	)	)	PUNCT
fnp-2749	19	26	with	with	ADP
fnp-2749	19	27	0.1	0.1	NUM
fnp-2749	19	28	%	%	NOUN
fnp-2749	19	29	(	(	PUNCT
fnp-2749	19	30	v	v	NOUN
fnp-2749	19	31	/	/	SYM
fnp-2749	19	32	v	v	NOUN
fnp-2749	19	33	)	)	PUNCT
fnp-2749	19	34	tween-20	tween-20	PROPN
fnp-2749	19	35	(	(	PUNCT
fnp-2749	19	36	sigma	sigma	PROPN
fnp-2749	19	37	,	,	PUNCT
fnp-2749	19	38	cat	cat	NOUN
fnp-2749	19	39	#	#	NOUN
fnp-2749	19	40	p1379	p1379	PROPN
fnp-2749	19	41	)	)	PUNCT
fnp-2749	19	42	and	and	CCONJ
fnp-2749	19	43	5	5	NUM
fnp-2749	19	44	%	%	NOUN
fnp-2749	19	45	(	(	PUNCT
fnp-2749	19	46	w	w	NOUN
fnp-2749	19	47	/	/	SYM
fnp-2749	19	48	v	v	NOUN
fnp-2749	19	49	)	)	PUNCT
fnp-2749	19	50	non	non	ADJ
fnp-2749	19	51	-	-	ADJ
fnp-2749	19	52	fat	fat	ADJ
fnp-2749	19	53	dry	dry	ADJ
fnp-2749	19	54	milk	milk	NOUN
fnp-2749	19	55	,	,	PUNCT
fnp-2749	19	56	then	then	ADV
fnp-2749	19	57	incubated	incubate	VERB
fnp-2749	19	58	overnight	overnight	ADV
fnp-2749	19	59	at	at	ADP
fnp-2749	19	60	4	4	NUM
fnp-2749	19	61	°	°	ADP
fnp-2749	19	62	c	c	NOUN
fnp-2749	19	63	with	with	ADP
fnp-2749	19	64	the	the	DET
fnp-2749	19	65	primary	primary	ADJ
fnp-2749	19	66	antibodies	antibody	NOUN
fnp-2749	19	67	listed	list	VERB
fnp-2749	19	68	in	in	ADP
fnp-2749	19	69	table	table	NOUN
fnp-2749	19	70	1	1	NUM
fnp-2749	19	71	.	.	PUNCT
fnp-2749	19	72	bound	bind	VERB
fnp-2749	19	73	antibodies	antibody	NOUN
fnp-2749	19	74	were	be	AUX
fnp-2749	19	75	detected	detect	VERB
fnp-2749	19	76	with	with	ADP
fnp-2749	19	77	horseradish	horseradish	ADJ
fnp-2749	19	78	peroxidase	peroxidase	NOUN
fnp-2749	19	79	-	-	PUNCT
fnp-2749	19	80	conjugated	conjugate	VERB
fnp-2749	19	81	anti	anti	ADJ
fnp-2749	19	82	-	-	ADJ
fnp-2749	19	83	rabbit	rabbit	ADJ
fnp-2749	19	84	(	(	PUNCT
fnp-2749	19	85	life	life	NOUN
fnp-2749	19	86	technologies	technology	NOUN
fnp-2749	19	87	cat	cat	NOUN
fnp-2749	19	88	#	#	NOUN
fnp-2749	19	89	31460	31460	NUM
fnp-2749	19	90	,	,	PUNCT
fnp-2749	19	91	diluted	dilute	VERB
fnp-2749	19	92	1:5,000	1:5,000	NUM
fnp-2749	19	93	)	)	PUNCT
fnp-2749	19	94	or	or	CCONJ
fnp-2749	19	95	anti	anti	ADJ
fnp-2749	19	96	-	-	ADJ
fnp-2749	19	97	mouse	mouse	ADJ
fnp-2749	19	98	antibodies	antibody	NOUN
fnp-2749	19	99	(	(	PUNCT
fnp-2749	19	100	sigma	sigma	PROPN
fnp-2749	19	101	,	,	PUNCT
fnp-2749	19	102	cat	cat	NOUN
fnp-2749	19	103	#	#	NOUN
fnp-2749	19	104	a9044	a9044	PROPN
fnp-2749	19	105	,	,	PUNCT
fnp-2749	19	106	diluted	dilute	VERB
fnp-2749	19	107	1:5,000	1:5,000	NUM
fnp-2749	19	108	)	)	PUNCT
fnp-2749	19	109	and	and	CCONJ
fnp-2749	19	110	visualized	visualize	VERB
fnp-2749	19	111	by	by	ADP
fnp-2749	19	112	enhanced	enhance	VERB
fnp-2749	19	113	chemiluminescent	chemiluminescent	PROPN
fnp-2749	19	114	detection	detection	NOUN
fnp-2749	19	115	(	(	PUNCT
fnp-2749	19	116	supersignal	supersignal	ADJ
fnp-2749	19	117	™	™	ADJ
fnp-2749	19	118	west	west	ADJ
fnp-2749	19	119	pico	pico	PROPN
fnp-2749	19	120	plus	plus	CCONJ
fnp-2749	19	121	chemiluminescent	chemiluminescent	NOUN
fnp-2749	19	122	substrate	substrate	NOUN
fnp-2749	19	123	,	,	PUNCT
fnp-2749	19	124	thermofisher	thermofisher	ADJ
fnp-2749	19	125	scientific	scientific	ADJ
fnp-2749	19	126	,	,	PUNCT
fnp-2749	19	127	cat	cat	NOUN
fnp-2749	19	128	#	#	NOUN
fnp-2749	19	129	34580	34580	NUM
fnp-2749	19	130	)	)	PUNCT
fnp-2749	19	131	.	.	PUNCT
fnp-2749	20	1	oligomeric	oligomeric	ADJ
fnp-2749	20	2	aggregates	aggregate	NOUN
fnp-2749	20	3	of	of	ADP
fnp-2749	20	4	alpha	alpha	NOUN
fnp-2749	20	5	-	-	PUNCT
fnp-2749	20	6	synuclein	synuclein	NOUN
fnp-2749	20	7	,	,	PUNCT
fnp-2749	20	8	which	which	PRON
fnp-2749	20	9	were	be	AUX
fnp-2749	20	10	obtained	obtain	VERB
fnp-2749	20	11	by	by	ADP
fnp-2749	20	12	incubating	incubate	VERB
fnp-2749	20	13	monomeric	monomeric	ADJ
fnp-2749	20	14	recombinant	recombinant	ADJ
fnp-2749	20	15	alpha	alpha	NOUN
fnp-2749	20	16	-	-	PUNCT
fnp-2749	20	17	synuclein	synuclein	NOUN
fnp-2749	20	18	(	(	PUNCT
fnp-2749	20	19	sigma	sigma	PROPN
fnp-2749	20	20	,	,	PUNCT
fnp-2749	20	21	cat	cat	NOUN
fnp-2749	20	22	#	#	NOUN
fnp-2749	20	23	575001	575001	NUM
fnp-2749	20	24	)	)	PUNCT
fnp-2749	20	25	in	in	ADP
fnp-2749	20	26	phosphate	phosphate	NOUN
fnp-2749	20	27	-	-	PUNCT
fnp-2749	20	28	buffered	buffer	VERB
fnp-2749	20	29	saline	saline	NOUN
fnp-2749	20	30	under	under	ADP
fnp-2749	20	31	agitation	agitation	NOUN
fnp-2749	20	32	at	at	ADP
fnp-2749	20	33	a	a	DET
fnp-2749	20	34	concentration	concentration	NOUN
fnp-2749	20	35	of	of	ADP
fnp-2749	20	36	10	10	NUM
fnp-2749	20	37	mg	mg	NOUN
fnp-2749	20	38	/	/	SYM
fnp-2749	20	39	ml	ml	NOUN
fnp-2749	20	40	at	at	ADP
fnp-2749	20	41	37	37	NUM
fnp-2749	20	42	°	°	ADP
fnp-2749	20	43	c	c	NOUN
fnp-2749	20	44	for	for	ADP
fnp-2749	20	45	one	one	NUM
fnp-2749	20	46	week	week	NOUN
fnp-2749	20	47	,	,	PUNCT
fnp-2749	20	48	served	serve	VERB
fnp-2749	20	49	as	as	ADP
fnp-2749	20	50	a	a	DET
fnp-2749	20	51	control	control	NOUN
fnp-2749	20	52	[	[	X
fnp-2749	20	53	8	8	NUM
fnp-2749	20	54	]	]	PUNCT
fnp-2749	20	55	.	.	PUNCT
fnp-2749	21	1	table	table	NOUN
fnp-2749	21	2	1	1	NUM
fnp-2749	21	3	.	.	PUNCT
fnp-2749	22	1	alpha	alpha	NOUN
fnp-2749	22	2	-	-	PUNCT
fnp-2749	22	3	synuclein	synuclein	NOUN
fnp-2749	22	4	and	and	CCONJ
fnp-2749	22	5	phospho	phospho	ADJ
fnp-2749	22	6	-	-	PUNCT
fnp-2749	22	7	alpha	alpha	NOUN
fnp-2749	22	8	-	-	PUNCT
fnp-2749	22	9	synuclein	synuclein	NOUN
fnp-2749	22	10	antibodies	antibody	NOUN
fnp-2749	22	11	used	use	VERB
fnp-2749	22	12	in	in	ADP
fnp-2749	22	13	the	the	DET
fnp-2749	22	14	current	current	ADJ
fnp-2749	22	15	study	study	NOUN
fnp-2749	22	16	.	.	PUNCT
fnp-2749	23	1	when	when	SCONJ
fnp-2749	23	2	cellulose	cellulose	NOUN
fnp-2749	23	3	acetate	acetate	NOUN
fnp-2749	23	4	membranes	membrane	NOUN
fnp-2749	23	5	were	be	AUX
fnp-2749	23	6	used	use	VERB
fnp-2749	23	7	,	,	PUNCT
fnp-2749	23	8	recombinant	recombinant	ADJ
fnp-2749	23	9	oligomeric	oligomeric	ADJ
fnp-2749	23	10	alpha	alpha	NOUN
fnp-2749	23	11	-	-	PUNCT
fnp-2749	23	12	synuclein	synuclein	NOUN
fnp-2749	23	13	and	and	CCONJ
fnp-2749	23	14	dlb	dlb	PROPN
fnp-2749	23	15	lysates	lysate	NOUN
fnp-2749	23	16	were	be	AUX
fnp-2749	23	17	barely	barely	ADV
fnp-2749	23	18	or	or	CCONJ
fnp-2749	23	19	not	not	PART
fnp-2749	23	20	detected	detect	VERB
fnp-2749	23	21	by	by	ADP
fnp-2749	23	22	two	two	NUM
fnp-2749	23	23	of	of	ADP
fnp-2749	23	24	the	the	DET
fnp-2749	23	25	total	total	ADJ
fnp-2749	23	26	alpha	alpha	NOUN
fnp-2749	23	27	-	-	PUNCT
fnp-2749	23	28	synuclein	synuclein	NOUN
fnp-2749	23	29	antibodies	antibodie	VERB
fnp-2749	23	30	(	(	PUNCT
fnp-2749	23	31	syn211	syn211	PROPN
fnp-2749	23	32	and	and	CCONJ
fnp-2749	23	33	syn-1	syn-1	NOUN
fnp-2749	23	34	)	)	PUNCT
fnp-2749	23	35	,	,	PUNCT
fnp-2749	23	36	regardless	regardless	ADV
fnp-2749	23	37	of	of	ADP
fnp-2749	23	38	the	the	DET
fnp-2749	23	39	lysis	lysis	NOUN
fnp-2749	23	40	procedure	procedure	NOUN
fnp-2749	23	41	(	(	PUNCT
fnp-2749	23	42	figure	figure	NOUN
fnp-2749	23	43	1a	1a	NOUN
fnp-2749	23	44	)	)	PUNCT
fnp-2749	23	45	.	.	PUNCT
fnp-2749	24	1	in	in	ADP
fnp-2749	24	2	contrast	contrast	NOUN
fnp-2749	24	3	,	,	PUNCT
fnp-2749	24	4	mjfr1	mjfr1	NOUN
fnp-2749	24	5	antibody	antibody	NOUN
fnp-2749	24	6	efficiently	efficiently	ADV
fnp-2749	24	7	detected	detect	VERB
fnp-2749	24	8	both	both	DET
fnp-2749	24	9	oligomeric	oligomeric	ADJ
fnp-2749	24	10	alpha	alpha	NOUN
fnp-2749	24	11	-	-	PUNCT
fnp-2749	24	12	synuclein	synuclein	NOUN
fnp-2749	24	13	and	and	CCONJ
fnp-2749	24	14	aggregated	aggregate	VERB
fnp-2749	24	15	alpha	alpha	NOUN
fnp-2749	24	16	-	-	PUNCT
fnp-2749	24	17	synuclein	synuclein	NOUN
fnp-2749	24	18	from	from	ADP
fnp-2749	24	19	ripa	ripa	NOUN
fnp-2749	24	20	-	-	PUNCT
fnp-2749	24	21	lysed	lyse	VERB
fnp-2749	24	22	dlb	dlb	NOUN
fnp-2749	24	23	samples	sample	NOUN
fnp-2749	24	24	(	(	PUNCT
fnp-2749	24	25	figure	figure	NOUN
fnp-2749	24	26	1a	1a	NOUN
fnp-2749	24	27	)	)	PUNCT
fnp-2749	24	28	.	.	PUNCT
fnp-2749	25	1	no	no	DET
fnp-2749	25	2	specific	specific	ADJ
fnp-2749	25	3	signal	signal	NOUN
fnp-2749	25	4	was	be	AUX
fnp-2749	25	5	observed	observe	VERB
fnp-2749	25	6	with	with	ADP
fnp-2749	25	7	this	this	DET
fnp-2749	25	8	antibody	antibody	NOUN
fnp-2749	25	9	when	when	SCONJ
fnp-2749	25	10	dlb	dlb	NOUN
fnp-2749	25	11	samples	sample	NOUN
fnp-2749	25	12	were	be	AUX
fnp-2749	25	13	lysed	lyse	VERB
fnp-2749	25	14	with	with	ADP
fnp-2749	25	15	sds	sds	PROPN
fnp-2749	25	16	or	or	CCONJ
fnp-2749	25	17	urea	urea	NOUN
fnp-2749	25	18	buffers	buffer	NOUN
fnp-2749	25	19	(	(	PUNCT
fnp-2749	25	20	figure	figure	NOUN
fnp-2749	25	21	1a	1a	NOUN
fnp-2749	25	22	)	)	PUNCT
fnp-2749	25	23	.	.	PUNCT
fnp-2749	26	1	two	two	NUM
fnp-2749	26	2	of	of	ADP
fnp-2749	26	3	the	the	DET
fnp-2749	26	4	phosphospecific	phosphospecific	ADJ
fnp-2749	26	5	antibodies	antibody	NOUN
fnp-2749	26	6	(	(	PUNCT
fnp-2749	26	7	ep1536y	ep1536y	PROPN
fnp-2749	26	8	and	and	CCONJ
fnp-2749	26	9	to	to	ADP
fnp-2749	26	10	a	a	DET
fnp-2749	26	11	lesser	less	ADJ
fnp-2749	26	12	extent	extent	NOUN
fnp-2749	26	13	psyn#64	psyn#64	PROPN
fnp-2749	26	14	)	)	PUNCT
fnp-2749	26	15	detected	detect	VERB
fnp-2749	26	16	aggregated	aggregate	VERB
fnp-2749	26	17	alpha	alpha	NOUN
fnp-2749	26	18	-	-	PUNCT
fnp-2749	26	19	synuclein	synuclein	NOUN
fnp-2749	26	20	in	in	ADP
fnp-2749	26	21	ripa	ripa	ADJ
fnp-2749	26	22	-	-	PUNCT
fnp-2749	26	23	dlb	dlb	NOUN
fnp-2749	26	24	lysates	lysate	NOUN
fnp-2749	26	25	.	.	PUNCT
fnp-2749	27	1	no	no	DET
fnp-2749	27	2	signal	signal	NOUN
fnp-2749	27	3	was	be	AUX
fnp-2749	27	4	observed	observe	VERB
fnp-2749	27	5	when	when	SCONJ
fnp-2749	27	6	psp	psp	NOUN
fnp-2749	27	7	and	and	CCONJ
fnp-2749	27	8	ad	ad	NOUN
fnp-2749	27	9	lysates	lysate	NOUN
fnp-2749	27	10	were	be	AUX
fnp-2749	27	11	filtered	filter	VERB
fnp-2749	27	12	and	and	CCONJ
fnp-2749	27	13	analyzed	analyze	VERB
fnp-2749	27	14	with	with	ADP
fnp-2749	27	15	either	either	PRON
fnp-2749	27	16	mjfr1	mjfr1	PROPN
fnp-2749	27	17	or	or	CCONJ
fnp-2749	27	18	ep1536y	ep1536y	ADJ
fnp-2749	27	19	antibody	antibody	NOUN
fnp-2749	27	20	.	.	PUNCT
fnp-2749	28	1	these	these	DET
fnp-2749	28	2	findings	finding	NOUN
fnp-2749	28	3	show	show	VERB
fnp-2749	28	4	that	that	SCONJ
fnp-2749	28	5	sample	sample	NOUN
fnp-2749	28	6	lysis	lysis	NOUN
fnp-2749	28	7	with	with	ADP
fnp-2749	28	8	ripa	ripa	PROPN
fnp-2749	28	9	sample	sample	NOUN
fnp-2749	28	10	lysis	lysis	NOUN
fnp-2749	28	11	together	together	ADV
fnp-2749	28	12	with	with	ADP
fnp-2749	28	13	mjfr1	mjfr1	PROPN
fnp-2749	28	14	and/or	and/or	CCONJ
fnp-2749	28	15	ep1536y	ep1536y	PROPN
fnp-2749	28	16	immunoblotting	immunoblotting	NOUN
fnp-2749	28	17	is	be	AUX
fnp-2749	28	18	the	the	DET
fnp-2749	28	19	most	most	ADV
fnp-2749	28	20	specific	specific	ADJ
fnp-2749	28	21	and	and	CCONJ
fnp-2749	28	22	reproducible	reproducible	ADJ
fnp-2749	28	23	approach	approach	NOUN
fnp-2749	28	24	.	.	PUNCT
fnp-2749	29	1	we	we	PRON
fnp-2749	29	2	therefore	therefore	ADV
fnp-2749	29	3	used	use	VERB
fnp-2749	29	4	these	these	DET
fnp-2749	29	5	two	two	NUM
fnp-2749	29	6	antibodies	antibody	NOUN
fnp-2749	29	7	and	and	CCONJ
fnp-2749	29	8	ripa	ripa	NOUN
fnp-2749	29	9	-	-	PUNCT
fnp-2749	29	10	lysed	lyse	VERB
fnp-2749	29	11	samples	sample	NOUN
fnp-2749	29	12	to	to	PART
fnp-2749	29	13	perform	perform	VERB
fnp-2749	29	14	additional	additional	ADJ
fnp-2749	29	15	dot	dot	NOUN
fnp-2749	29	16	blots	blot	NOUN
fnp-2749	29	17	with	with	ADP
fnp-2749	29	18	pvdf	pvdf	PROPN
fnp-2749	29	19	or	or	CCONJ
fnp-2749	29	20	nitrocellulose	nitrocellulose	NOUN
fnp-2749	29	21	membranes	membrane	NOUN
fnp-2749	29	22	,	,	PUNCT
fnp-2749	29	23	that	that	PRON
fnp-2749	29	24	could	could	AUX
fnp-2749	29	25	serve	serve	VERB
fnp-2749	29	26	as	as	ADP
fnp-2749	29	27	a	a	DET
fnp-2749	29	28	loading	loading	NOUN
fnp-2749	29	29	control	control	NOUN
fnp-2749	29	30	.	.	PUNCT
fnp-2749	30	1	the	the	DET
fnp-2749	30	2	results	result	NOUN
fnp-2749	30	3	show	show	VERB
fnp-2749	30	4	that	that	SCONJ
fnp-2749	30	5	pvdf	pvdf	PROPN
fnp-2749	30	6	achieved	achieve	VERB
fnp-2749	30	7	better	well	ADJ
fnp-2749	30	8	reproducibility	reproducibility	NOUN
fnp-2749	30	9	and	and	CCONJ
fnp-2749	30	10	specificity	specificity	NOUN
fnp-2749	30	11	for	for	ADP
fnp-2749	30	12	the	the	DET
fnp-2749	30	13	detection	detection	NOUN
fnp-2749	30	14	of	of	ADP
fnp-2749	30	15	total	total	ADJ
fnp-2749	30	16	and	and	CCONJ
fnp-2749	30	17	phosphorylated	phosphorylate	VERB
fnp-2749	30	18	alpha	alpha	NOUN
fnp-2749	30	19	-	-	PUNCT
fnp-2749	30	20	synuclein	synuclein	NOUN
fnp-2749	30	21	than	than	ADP
fnp-2749	30	22	nitrocellulose	nitrocellulose	NOUN
fnp-2749	30	23	membranes	membrane	NOUN
fnp-2749	30	24	(	(	PUNCT
fnp-2749	30	25	figure	figure	NOUN
fnp-2749	30	26	1b	1b	NUM
fnp-2749	30	27	)	)	PUNCT
fnp-2749	30	28	.	.	PUNCT
fnp-2749	31	1	figure	figure	NOUN
fnp-2749	31	2	1	1	NUM
fnp-2749	31	3	.	.	PUNCT
fnp-2749	31	4	results	result	NOUN
fnp-2749	31	5	from	from	ADP
fnp-2749	31	6	the	the	DET
fnp-2749	31	7	filter	filter	NOUN
fnp-2749	31	8	trap	trap	NOUN
fnp-2749	31	9	and	and	CCONJ
fnp-2749	31	10	dot	dot	NOUN
fnp-2749	31	11	blot	blot	NOUN
fnp-2749	31	12	assays	assay	NOUN
fnp-2749	31	13	.	.	PUNCT
fnp-2749	32	1	(	(	PUNCT
fnp-2749	32	2	a	a	X
fnp-2749	32	3	)	)	PUNCT
fnp-2749	32	4	recombinant	recombinant	ADJ
fnp-2749	32	5	oligomeric	oligomeric	ADJ
fnp-2749	32	6	alpha	alpha	NOUN
fnp-2749	32	7	-	-	PUNCT
fnp-2749	32	8	synuclein	synuclein	NOUN
fnp-2749	32	9	(	(	PUNCT
fnp-2749	32	10	oligo	oligo	ADJ
fnp-2749	32	11	)	)	PUNCT
fnp-2749	32	12	and	and	CCONJ
fnp-2749	32	13	samples	sample	NOUN
fnp-2749	32	14	from	from	ADP
fnp-2749	32	15	dlb	dlb	NOUN
fnp-2749	32	16	,	,	PUNCT
fnp-2749	32	17	psp	psp	NOUN
fnp-2749	32	18	and	and	CCONJ
fnp-2749	32	19	ad	ad	NOUN
fnp-2749	32	20	brains	brain	NOUN
fnp-2749	32	21	lysed	lyse	VERB
fnp-2749	32	22	in	in	ADP
fnp-2749	32	23	ripa	ripa	PROPN
fnp-2749	32	24	,	,	PUNCT
fnp-2749	32	25	sds	sds	PROPN
fnp-2749	32	26	and	and	CCONJ
fnp-2749	32	27	urea	urea	NOUN
fnp-2749	32	28	buffers	buffer	NOUN
fnp-2749	32	29	were	be	AUX
fnp-2749	32	30	subjected	subject	VERB
fnp-2749	32	31	to	to	ADP
fnp-2749	32	32	vacuum	vacuum	VERB
fnp-2749	32	33	filtration	filtration	NOUN
fnp-2749	32	34	through	through	ADP
fnp-2749	32	35	a	a	DET
fnp-2749	32	36	96	96	NUM
fnp-2749	32	37	-	-	PUNCT
fnp-2749	32	38	well	well	NOUN
fnp-2749	32	39	dot	dot	NOUN
fnp-2749	32	40	blot	blot	NOUN
fnp-2749	32	41	apparatus	apparatus	NOUN
fnp-2749	32	42	with	with	ADP
fnp-2749	32	43	an	an	DET
fnp-2749	32	44	acetate	acetate	NOUN
fnp-2749	32	45	cellulose	cellulose	NOUN
fnp-2749	32	46	membrane	membrane	NOUN
fnp-2749	32	47	;	;	PUNCT
fnp-2749	32	48	immunoblotting	immunoblotte	VERB
fnp-2749	32	49	(	(	PUNCT
fnp-2749	32	50	ib	ib	NOUN
fnp-2749	32	51	)	)	PUNCT
fnp-2749	32	52	was	be	AUX
fnp-2749	32	53	then	then	ADV
fnp-2749	32	54	performed	perform	VERB
fnp-2749	32	55	with	with	ADP
fnp-2749	32	56	3	3	NUM
fnp-2749	32	57	different	different	ADJ
fnp-2749	32	58	antibodies	antibody	NOUN
fnp-2749	32	59	to	to	PART
fnp-2749	32	60	total	total	VERB
fnp-2749	32	61	alpha	alpha	NOUN
fnp-2749	32	62	-	-	PUNCT
fnp-2749	32	63	synuclein	synuclein	NOUN
fnp-2749	32	64	(	(	PUNCT
fnp-2749	32	65	mjfr1	mjfr1	NOUN
fnp-2749	32	66	,	,	PUNCT
fnp-2749	32	67	syn-1	syn-1	PUNCT
fnp-2749	32	68	and	and	CCONJ
fnp-2749	32	69	syn211	syn211	PROPN
fnp-2749	32	70	)	)	PUNCT
fnp-2749	32	71	and	and	CCONJ
fnp-2749	32	72	3	3	NUM
fnp-2749	32	73	antibodies	antibodie	VERB
fnp-2749	32	74	specific	specific	ADJ
fnp-2749	32	75	for	for	ADP
fnp-2749	32	76	the	the	DET
fnp-2749	32	77	phosphorylated	phosphorylate	VERB
fnp-2749	32	78	form	form	NOUN
fnp-2749	32	79	of	of	ADP
fnp-2749	32	80	the	the	DET
fnp-2749	32	81	protein	protein	NOUN
fnp-2749	32	82	(	(	PUNCT
fnp-2749	32	83	ep1536y	ep1536y	PROPN
fnp-2749	32	84	,	,	PUNCT
fnp-2749	32	85	psyn#64	psyn#64	PROPN
fnp-2749	32	86	and	and	CCONJ
fnp-2749	32	87	phospho	phospho	ADJ
fnp-2749	32	88	s129	s129	PROPN
fnp-2749	32	89	)	)	PUNCT
fnp-2749	32	90	.	.	PUNCT
fnp-2749	33	1	(	(	PUNCT
fnp-2749	33	2	b	b	X
fnp-2749	33	3	)	)	PUNCT
fnp-2749	33	4	recombinant	recombinant	ADJ
fnp-2749	33	5	oligomeric	oligomeric	ADJ
fnp-2749	33	6	alpha	alpha	NOUN
fnp-2749	33	7	-	-	PUNCT
fnp-2749	33	8	synuclein	synuclein	NOUN
fnp-2749	33	9	(	(	PUNCT
fnp-2749	33	10	oligo	oligo	ADJ
fnp-2749	33	11	)	)	PUNCT
fnp-2749	33	12	and	and	CCONJ
fnp-2749	33	13	ripa	ripa	NOUN
fnp-2749	33	14	-	-	PUNCT
fnp-2749	33	15	lysed	lyse	VERB
fnp-2749	33	16	samples	sample	NOUN
fnp-2749	33	17	from	from	ADP
fnp-2749	33	18	dlb	dlb	NOUN
fnp-2749	33	19	,	,	PUNCT
fnp-2749	33	20	psp	psp	NOUN
fnp-2749	33	21	and	and	CCONJ
fnp-2749	33	22	ad	ad	NOUN
fnp-2749	33	23	brains	brain	NOUN
fnp-2749	33	24	were	be	AUX
fnp-2749	33	25	subjected	subject	VERB
fnp-2749	33	26	to	to	ADP
fnp-2749	33	27	vacuum	vacuum	VERB
fnp-2749	33	28	filtration	filtration	NOUN
fnp-2749	33	29	through	through	ADP
fnp-2749	33	30	a	a	DET
fnp-2749	33	31	96	96	NUM
fnp-2749	33	32	-	-	PUNCT
fnp-2749	33	33	well	well	NOUN
fnp-2749	33	34	dot	dot	NOUN
fnp-2749	33	35	blot	blot	NOUN
fnp-2749	33	36	apparatus	apparatus	NOUN
fnp-2749	33	37	with	with	ADP
fnp-2749	33	38	a	a	DET
fnp-2749	33	39	pvdf	pvdf	NOUN
fnp-2749	33	40	or	or	CCONJ
fnp-2749	33	41	a	a	DET
fnp-2749	33	42	nitrocellulose	nitrocellulose	NOUN
fnp-2749	33	43	membrane	membrane	NOUN
fnp-2749	33	44	;	;	PUNCT
fnp-2749	33	45	immunoblotting	immunoblotte	VERB
fnp-2749	33	46	(	(	PUNCT
fnp-2749	33	47	ib	ib	NOUN
fnp-2749	33	48	)	)	PUNCT
fnp-2749	33	49	was	be	AUX
fnp-2749	33	50	then	then	ADV
fnp-2749	33	51	performed	perform	VERB
fnp-2749	33	52	with	with	ADP
fnp-2749	33	53	mjfr1	mjfr1	PROPN
fnp-2749	33	54	or	or	CCONJ
fnp-2749	33	55	ep1536y	ep1536y	PROPN
fnp-2749	33	56	antibodies	antibodie	VERB
fnp-2749	33	57	.	.	PUNCT
fnp-2749	34	1	results	result	NOUN
fnp-2749	34	2	shown	show	VERB
fnp-2749	34	3	are	be	AUX
fnp-2749	34	4	representative	representative	NOUN
fnp-2749	34	5	of	of	ADP
fnp-2749	34	6	3	3	NUM
fnp-2749	34	7	to	to	PART
fnp-2749	34	8	5	5	NUM
fnp-2749	34	9	independent	independent	ADJ
fnp-2749	34	10	experiments	experiment	NOUN
fnp-2749	34	11	.	.	PUNCT
fnp-2749	35	1	the	the	DET
fnp-2749	35	2	results	result	NOUN
fnp-2749	35	3	presented	present	VERB
fnp-2749	35	4	here	here	ADV
fnp-2749	35	5	,	,	PUNCT
fnp-2749	35	6	although	although	SCONJ
fnp-2749	35	7	preliminary	preliminary	ADJ
fnp-2749	35	8	,	,	PUNCT
fnp-2749	35	9	provide	provide	VERB
fnp-2749	35	10	the	the	DET
fnp-2749	35	11	basis	basis	NOUN
fnp-2749	35	12	for	for	ADP
fnp-2749	35	13	reproducible	reproducible	ADJ
fnp-2749	35	14	and	and	CCONJ
fnp-2749	35	15	specific	specific	ADJ
fnp-2749	35	16	detection	detection	NOUN
fnp-2749	35	17	of	of	ADP
fnp-2749	35	18	pathological	pathological	ADJ
fnp-2749	35	19	alpha	alpha	NOUN
fnp-2749	35	20	-	-	PUNCT
fnp-2749	35	21	synuclein	synuclein	NOUN
fnp-2749	35	22	in	in	ADP
fnp-2749	35	23	diseased	diseased	ADJ
fnp-2749	35	24	brains	brain	NOUN
fnp-2749	35	25	using	use	VERB
fnp-2749	35	26	a	a	DET
fnp-2749	35	27	filter	filter	NOUN
fnp-2749	35	28	trap	trap	NOUN
fnp-2749	35	29	assay	assay	NOUN
fnp-2749	35	30	.	.	PUNCT
fnp-2749	36	1	we	we	PRON
fnp-2749	36	2	show	show	VERB
fnp-2749	36	3	that	that	SCONJ
fnp-2749	36	4	the	the	DET
fnp-2749	36	5	selection	selection	NOUN
fnp-2749	36	6	of	of	ADP
fnp-2749	36	7	primary	primary	ADJ
fnp-2749	36	8	antibody	antibody	NOUN
fnp-2749	36	9	and	and	CCONJ
fnp-2749	36	10	lysis	lysis	NOUN
fnp-2749	36	11	buffer	buffer	NOUN
fnp-2749	36	12	has	have	VERB
fnp-2749	36	13	important	important	ADJ
fnp-2749	36	14	effects	effect	NOUN
fnp-2749	36	15	on	on	ADP
fnp-2749	36	16	the	the	DET
fnp-2749	36	17	performance	performance	NOUN
fnp-2749	36	18	of	of	ADP
fnp-2749	36	19	the	the	DET
fnp-2749	36	20	assay	assay	NOUN
fnp-2749	36	21	.	.	PUNCT
fnp-2749	37	1	as	as	ADP
fnp-2749	37	2	an	an	DET
fnp-2749	37	3	optimized	optimize	VERB
fnp-2749	37	4	protocol	protocol	NOUN
fnp-2749	37	5	,	,	PUNCT
fnp-2749	37	6	we	we	PRON
fnp-2749	37	7	suggest	suggest	VERB
fnp-2749	37	8	that	that	SCONJ
fnp-2749	37	9	brain	brain	NOUN
fnp-2749	37	10	homogenates	homogenate	NOUN
fnp-2749	37	11	should	should	AUX
fnp-2749	37	12	be	be	AUX
fnp-2749	37	13	lysed	lyse	VERB
fnp-2749	37	14	in	in	ADP
fnp-2749	37	15	ripa	ripa	ADJ
fnp-2749	37	16	buffer	buffer	NOUN
fnp-2749	37	17	and	and	CCONJ
fnp-2749	37	18	that	that	SCONJ
fnp-2749	37	19	immunoblotting	immunoblotting	NOUN
fnp-2749	37	20	should	should	AUX
fnp-2749	37	21	be	be	AUX
fnp-2749	37	22	performed	perform	VERB
fnp-2749	37	23	with	with	ADP
fnp-2749	37	24	mjfr1	mjfr1	NOUN
fnp-2749	37	25	for	for	ADP
fnp-2749	37	26	the	the	DET
fnp-2749	37	27	detection	detection	NOUN
fnp-2749	37	28	of	of	ADP
fnp-2749	37	29	total	total	ADJ
fnp-2749	37	30	alpha	alpha	NOUN
fnp-2749	37	31	-	-	PUNCT
fnp-2749	37	32	synuclein	synuclein	NOUN
fnp-2749	37	33	and	and	CCONJ
fnp-2749	37	34	ep1536y	ep1536y	PROPN
fnp-2749	37	35	for	for	ADP
fnp-2749	37	36	the	the	DET
fnp-2749	37	37	detection	detection	NOUN
fnp-2749	37	38	of	of	ADP
fnp-2749	37	39	the	the	DET
fnp-2749	37	40	phosphorylated	phosphorylate	VERB
fnp-2749	37	41	form	form	NOUN
fnp-2749	37	42	of	of	ADP
fnp-2749	37	43	the	the	DET
fnp-2749	37	44	protein	protein	NOUN
fnp-2749	37	45	(	(	PUNCT
fnp-2749	37	46	a	a	DET
fnp-2749	37	47	step	step	NOUN
fnp-2749	37	48	by	by	ADP
fnp-2749	37	49	step	step	NOUN
fnp-2749	37	50	standard	standard	ADJ
fnp-2749	37	51	operating	operating	NOUN
fnp-2749	37	52	procedure	procedure	NOUN
fnp-2749	37	53	is	be	AUX
fnp-2749	37	54	provided	provide	VERB
fnp-2749	37	55	as	as	ADP
fnp-2749	37	56	supplementary	supplementary	ADJ
fnp-2749	37	57	material	material	NOUN
fnp-2749	37	58	)	)	PUNCT
fnp-2749	37	59	.	.	PUNCT
fnp-2749	38	1	owing	owe	VERB
fnp-2749	38	2	to	to	ADP
fnp-2749	38	3	the	the	DET
fnp-2749	38	4	high	high	ADJ
fnp-2749	38	5	-	-	PUNCT
fnp-2749	38	6	throughput	throughput	NOUN
fnp-2749	38	7	capability	capability	NOUN
fnp-2749	38	8	of	of	ADP
fnp-2749	38	9	the	the	DET
fnp-2749	38	10	filter	filter	NOUN
fnp-2749	38	11	trap	trap	NOUN
fnp-2749	38	12	assay	assay	NOUN
fnp-2749	38	13	,	,	PUNCT
fnp-2749	38	14	such	such	DET
fnp-2749	38	15	an	an	DET
fnp-2749	38	16	approach	approach	NOUN
fnp-2749	38	17	is	be	AUX
fnp-2749	38	18	not	not	PART
fnp-2749	38	19	only	only	ADV
fnp-2749	38	20	potentially	potentially	ADV
fnp-2749	38	21	interesting	interesting	ADJ
fnp-2749	38	22	for	for	ADP
fnp-2749	38	23	detecting	detect	VERB
fnp-2749	38	24	aggregated	aggregate	VERB
fnp-2749	38	25	alpha	alpha	NOUN
fnp-2749	38	26	-	-	PUNCT
fnp-2749	38	27	synuclein	synuclein	NOUN
fnp-2749	38	28	in	in	ADP
fnp-2749	38	29	post	post	X
fnp-2749	38	30	mortem	mortem	X
fnp-2749	38	31	brain	brain	NOUN
fnp-2749	38	32	samples	sample	NOUN
fnp-2749	38	33	but	but	CCONJ
fnp-2749	38	34	also	also	ADV
fnp-2749	38	35	in	in	ADP
fnp-2749	38	36	the	the	DET
fnp-2749	38	37	peripheral	peripheral	ADJ
fnp-2749	38	38	nervous	nervous	ADJ
fnp-2749	38	39	system	system	NOUN
fnp-2749	38	40	of	of	ADP
fnp-2749	38	41	living	live	VERB
fnp-2749	38	42	pd	pd	PROPN
fnp-2749	38	43	patients	patient	NOUN
fnp-2749	38	44	in	in	ADP
fnp-2749	38	45	order	order	NOUN
fnp-2749	38	46	to	to	PART
fnp-2749	38	47	develop	develop	VERB
fnp-2749	38	48	original	original	ADJ
fnp-2749	38	49	biomarkers	biomarker	NOUN
fnp-2749	38	50	of	of	ADP
fnp-2749	38	51	the	the	DET
fnp-2749	38	52	disease	disease	NOUN
fnp-2749	38	53	[	[	X
fnp-2749	38	54	9	9	NUM
fnp-2749	38	55	]	]	PUNCT
fnp-2749	38	56	.	.	PUNCT
fnp-2749	39	1	references	reference	NOUN
fnp-2749	39	2	1	1	NUM
fnp-2749	39	3	.	.	PUNCT
fnp-2749	39	4	kovacs	kovacs	PROPN
fnp-2749	39	5	gg	gg	PROPN
fnp-2749	39	6	.	.	PUNCT
fnp-2749	40	1	molecular	molecular	ADJ
fnp-2749	40	2	pathological	pathological	ADJ
fnp-2749	40	3	classification	classification	NOUN
fnp-2749	40	4	of	of	ADP
fnp-2749	40	5	neurodegenerative	neurodegenerative	ADJ
fnp-2749	40	6	diseases	disease	NOUN
fnp-2749	40	7	:	:	PUNCT
fnp-2749	40	8	turning	turn	VERB
fnp-2749	40	9	towards	towards	ADP
fnp-2749	40	10	precision	precision	NOUN
fnp-2749	40	11	medicine	medicine	NOUN
fnp-2749	40	12	.	.	PUNCT
fnp-2749	41	1	int	int	PROPN
fnp-2749	41	2	j	j	PROPN
fnp-2749	41	3	mol	mol	ADP
fnp-2749	41	4	sci	sci	PROPN
fnp-2749	41	5	.	.	PUNCT
fnp-2749	42	1	2016;17:189	2016;17:189	X
fnp-2749	42	2	.	.	PUNCT
fnp-2749	42	3	2	2	NUM
fnp-2749	42	4	.	.	X
fnp-2749	42	5	bieschke	bieschke	PROPN
fnp-2749	42	6	j	j	PROPN
fnp-2749	42	7	,	,	PUNCT
fnp-2749	42	8	cohen	cohen	PROPN
fnp-2749	42	9	e	e	PROPN
fnp-2749	42	10	,	,	PUNCT
fnp-2749	42	11	murray	murray	PROPN
fnp-2749	42	12	a	a	PRON
fnp-2749	42	13	,	,	PUNCT
fnp-2749	42	14	dillin	dillin	PROPN
fnp-2749	42	15	a	a	PROPN
fnp-2749	42	16	,	,	PUNCT
fnp-2749	42	17	kelly	kelly	PROPN
fnp-2749	42	18	jw	jw	PROPN
fnp-2749	42	19	.	.	PUNCT
fnp-2749	43	1	a	a	DET
fnp-2749	43	2	kinetic	kinetic	ADJ
fnp-2749	43	3	assessment	assessment	NOUN
fnp-2749	43	4	of	of	ADP
fnp-2749	43	5	the	the	DET
fnp-2749	43	6	c.	c.	PROPN
fnp-2749	43	7	elegans	elegans	PROPN
fnp-2749	43	8	amyloid	amyloid	NOUN
fnp-2749	43	9	disaggregation	disaggregation	NOUN
fnp-2749	43	10	activity	activity	NOUN
fnp-2749	43	11	enables	enable	VERB
fnp-2749	43	12	uncoupling	uncouple	VERB
fnp-2749	43	13	of	of	ADP
fnp-2749	43	14	disassembly	disassembly	NOUN
fnp-2749	43	15	and	and	CCONJ
fnp-2749	43	16	proteolysis	proteolysis	NOUN
fnp-2749	43	17	.	.	PUNCT
fnp-2749	44	1	protein	protein	NOUN
fnp-2749	44	2	sci	sci	PROPN
fnp-2749	44	3	.	.	PUNCT
fnp-2749	44	4	2009;18:2231–41	2009;18:2231–41	NUM
fnp-2749	44	5	.	.	PUNCT
fnp-2749	45	1	3	3	X
fnp-2749	45	2	.	.	X
fnp-2749	45	3	boyé	boyé	NOUN
fnp-2749	45	4	-	-	PUNCT
fnp-2749	45	5	harnasch	harnasch	PROPN
fnp-2749	45	6	m	m	PROPN
fnp-2749	45	7	,	,	PUNCT
fnp-2749	45	8	cullin	cullin	PROPN
fnp-2749	45	9	c.	c.	PROPN
fnp-2749	45	10	a	a	DET
fnp-2749	45	11	novel	novel	NOUN
fnp-2749	45	12	in	in	ADP
fnp-2749	45	13	vitro	vitro	X
fnp-2749	45	14	filter	filter	NOUN
fnp-2749	45	15	trap	trap	NOUN
fnp-2749	45	16	assay	assay	NOUN
fnp-2749	45	17	identifies	identify	VERB
fnp-2749	45	18	tannic	tannic	ADJ
fnp-2749	45	19	acid	acid	NOUN
fnp-2749	45	20	as	as	ADP
fnp-2749	45	21	an	an	DET
fnp-2749	45	22	amyloid	amyloid	NOUN
fnp-2749	45	23	aggregation	aggregation	NOUN
fnp-2749	45	24	inducer	inducer	NOUN
fnp-2749	45	25	for	for	ADP
fnp-2749	45	26	het	het	PROPN
fnp-2749	45	27	-	-	PUNCT
fnp-2749	45	28	s.	s.	PROPN
fnp-2749	45	29	j	j	PROPN
fnp-2749	45	30	biotechnol	biotechnol	PROPN
fnp-2749	45	31	.	.	PUNCT
fnp-2749	46	1	2006;125:222–30	2006;125:222–30	NUM
fnp-2749	46	2	.	.	PUNCT
fnp-2749	47	1	4	4	NUM
fnp-2749	47	2	.	.	X
fnp-2749	47	3	chang	chang	PROPN
fnp-2749	47	4	e	e	PROPN
fnp-2749	47	5	,	,	PUNCT
fnp-2749	47	6	kuret	kuret	PROPN
fnp-2749	47	7	j.	j.	PROPN
fnp-2749	47	8	detection	detection	PROPN
fnp-2749	47	9	and	and	CCONJ
fnp-2749	47	10	quantification	quantification	NOUN
fnp-2749	47	11	of	of	ADP
fnp-2749	47	12	tau	tau	PROPN
fnp-2749	47	13	aggregation	aggregation	NOUN
fnp-2749	47	14	using	use	VERB
fnp-2749	47	15	a	a	DET
fnp-2749	47	16	membrane	membrane	NOUN
fnp-2749	47	17	filter	filter	NOUN
fnp-2749	47	18	assay	assay	NOUN
fnp-2749	47	19	.	.	PUNCT
fnp-2749	48	1	anal	anal	ADJ
fnp-2749	48	2	biochem	biochem	PROPN
fnp-2749	48	3	.	.	PUNCT
fnp-2749	49	1	2008;373:330–6	2008;373:330–6	NOUN
fnp-2749	49	2	.	.	PUNCT
fnp-2749	50	1	5	5	NUM
fnp-2749	50	2	.	.	X
fnp-2749	50	3	novoselova	novoselova	PROPN
fnp-2749	50	4	tv	tv	PROPN
fnp-2749	50	5	,	,	PUNCT
fnp-2749	50	6	margulis	margulis	PROPN
fnp-2749	50	7	ba	ba	PROPN
fnp-2749	50	8	,	,	PUNCT
fnp-2749	50	9	novoselov	novoselov	PROPN
fnp-2749	50	10	ss	ss	PROPN
fnp-2749	50	11	,	,	PUNCT
fnp-2749	50	12	sapozhnikov	sapozhnikov	PROPN
fnp-2749	50	13	am	be	AUX
fnp-2749	50	14	,	,	PUNCT
fnp-2749	50	15	van	van	PROPN
fnp-2749	50	16	der	der	PROPN
fnp-2749	50	17	spuy	spuy	PROPN
fnp-2749	50	18	j	j	PROPN
fnp-2749	50	19	,	,	PUNCT
fnp-2749	50	20	cheetham	cheetham	PROPN
fnp-2749	50	21	me	i	PRON
fnp-2749	50	22	,	,	PUNCT
fnp-2749	50	23	et	et	PROPN
fnp-2749	50	24	al	al	PROPN
fnp-2749	50	25	.	.	PUNCT
fnp-2749	50	26	treatment	treatment	NOUN
fnp-2749	50	27	with	with	ADP
fnp-2749	50	28	extracellular	extracellular	ADJ
fnp-2749	50	29	hsp70	hsp70	NOUN
fnp-2749	50	30	/	/	SYM
fnp-2749	50	31	hsc70	hsc70	ADJ
fnp-2749	50	32	protein	protein	NOUN
fnp-2749	50	33	can	can	AUX
fnp-2749	50	34	reduce	reduce	VERB
fnp-2749	50	35	polyglutamine	polyglutamine	NOUN
fnp-2749	50	36	toxicity	toxicity	NOUN
fnp-2749	50	37	and	and	CCONJ
fnp-2749	50	38	aggregation	aggregation	NOUN
fnp-2749	50	39	.	.	PUNCT
fnp-2749	51	1	j	j	PROPN
fnp-2749	51	2	neurochem	neurochem	PROPN
fnp-2749	51	3	.	.	PUNCT
fnp-2749	52	1	2005;94:597–606	2005;94:597–606	NUM
fnp-2749	52	2	.	.	NOUN
fnp-2749	53	1	6	6	NUM
fnp-2749	53	2	.	.	X
fnp-2749	54	1	van	van	PROPN
fnp-2749	54	2	waarde	waarde	PROPN
fnp-2749	54	3	-	-	PUNCT
fnp-2749	54	4	verhagen	verhagen	PROPN
fnp-2749	54	5	mawh	mawh	NOUN
fnp-2749	54	6	,	,	PUNCT
fnp-2749	54	7	kampinga	kampinga	NOUN
fnp-2749	54	8	hh	hh	PROPN
fnp-2749	54	9	.	.	PROPN
fnp-2749	54	10	measurement	measurement	PROPN
fnp-2749	54	11	of	of	ADP
fnp-2749	54	12	chaperone	chaperone	NOUN
fnp-2749	54	13	-	-	PUNCT
fnp-2749	54	14	mediated	mediate	VERB
fnp-2749	54	15	effects	effect	NOUN
fnp-2749	54	16	on	on	ADP
fnp-2749	54	17	polyglutamine	polyglutamine	NOUN
fnp-2749	54	18	protein	protein	NOUN
fnp-2749	54	19	aggregation	aggregation	NOUN
fnp-2749	54	20	by	by	ADP
fnp-2749	54	21	the	the	DET
fnp-2749	54	22	filter	filter	NOUN
fnp-2749	54	23	trap	trap	NOUN
fnp-2749	54	24	assay	assay	NOUN
fnp-2749	54	25	.	.	PUNCT
fnp-2749	55	1	methods	method	NOUN
fnp-2749	55	2	mol	mol	ADP
fnp-2749	55	3	biol	biol	PROPN
fnp-2749	55	4	.	.	PUNCT
fnp-2749	56	1	2018;1709:59–74	2018;1709:59–74	X
fnp-2749	56	2	.	.	NOUN
fnp-2749	57	1	7	7	NUM
fnp-2749	57	2	.	.	NUM
fnp-2749	57	3	recasens	recasen	NOUN
fnp-2749	57	4	a	a	PRON
fnp-2749	57	5	,	,	PUNCT
fnp-2749	57	6	dehay	dehay	PROPN
fnp-2749	57	7	b	b	PROPN
fnp-2749	57	8	,	,	PUNCT
fnp-2749	57	9	bové	bové	PROPN
fnp-2749	57	10	j	j	NOUN
fnp-2749	57	11	,	,	PUNCT
fnp-2749	57	12	carballo	carballo	NOUN
fnp-2749	57	13	-	-	PUNCT
fnp-2749	57	14	carbajal	carbajal	NOUN
fnp-2749	57	15	i	i	NOUN
fnp-2749	57	16	,	,	PUNCT
fnp-2749	57	17	dovero	dovero	NOUN
fnp-2749	57	18	s	s	PROPN
fnp-2749	57	19	,	,	PUNCT
fnp-2749	57	20	pérez	pérez	NOUN
fnp-2749	57	21	-	-	PUNCT
fnp-2749	57	22	villalba	villalba	NOUN
fnp-2749	57	23	a	a	NOUN
fnp-2749	57	24	,	,	PUNCT
fnp-2749	57	25	et	et	PROPN
fnp-2749	57	26	al	al	PROPN
fnp-2749	57	27	.	.	PROPN
fnp-2749	57	28	lewy	lewy	PROPN
fnp-2749	57	29	body	body	NOUN
fnp-2749	57	30	extracts	extract	NOUN
fnp-2749	57	31	from	from	ADP
fnp-2749	57	32	parkinson	parkinson	NOUN
fnp-2749	57	33	disease	disease	NOUN
fnp-2749	57	34	brains	brain	NOUN
fnp-2749	57	35	trigger	trigger	VERB
fnp-2749	57	36	α	α	NOUN
fnp-2749	57	37	-	-	PUNCT
fnp-2749	57	38	synuclein	synuclein	NOUN
fnp-2749	57	39	pathology	pathology	NOUN
fnp-2749	57	40	and	and	CCONJ
fnp-2749	57	41	neurodegeneration	neurodegeneration	NOUN
fnp-2749	57	42	in	in	ADP
fnp-2749	57	43	mice	mouse	NOUN
fnp-2749	57	44	and	and	CCONJ
fnp-2749	57	45	monkeys	monkey	NOUN
fnp-2749	57	46	.	.	PUNCT
fnp-2749	58	1	ann	ann	PROPN
fnp-2749	58	2	neurol	neurol	PROPN
fnp-2749	58	3	.	.	PUNCT
fnp-2749	59	1	2014;75:351–62	2014;75:351–62	X
fnp-2749	59	2	.	.	NOUN
fnp-2749	59	3	8	8	NUM
fnp-2749	59	4	.	.	X
fnp-2749	59	5	corbillé	corbillé	VERB
fnp-2749	59	6	a	a	DET
fnp-2749	59	7	-	-	PUNCT
fnp-2749	59	8	g	g	NOUN
fnp-2749	59	9	,	,	PUNCT
fnp-2749	59	10	neunlist	neunlist	NOUN
fnp-2749	59	11	m	m	PROPN
fnp-2749	59	12	,	,	PUNCT
fnp-2749	59	13	derkinderen	derkinderen	PROPN
fnp-2749	59	14	p.	p.	PROPN
fnp-2749	59	15	cross	cross	NOUN
fnp-2749	59	16	-	-	ADJ
fnp-2749	59	17	linking	link	VERB
fnp-2749	59	18	for	for	ADP
fnp-2749	59	19	the	the	DET
fnp-2749	59	20	analysis	analysis	NOUN
fnp-2749	59	21	of	of	ADP
fnp-2749	59	22	α	α	NOUN
fnp-2749	59	23	-	-	PUNCT
fnp-2749	59	24	synuclein	synuclein	NOUN
fnp-2749	59	25	in	in	ADP
fnp-2749	59	26	the	the	DET
fnp-2749	59	27	enteric	enteric	ADJ
fnp-2749	59	28	nervous	nervous	ADJ
fnp-2749	59	29	system	system	NOUN
fnp-2749	59	30	.	.	PUNCT
fnp-2749	60	1	j	j	PROPN
fnp-2749	60	2	neurochem	neurochem	PROPN
fnp-2749	60	3	.	.	PUNCT
fnp-2749	61	1	2016;139:839–47	2016;139:839–47	VERB
fnp-2749	61	2	.	.	NOUN
fnp-2749	61	3	9	9	NUM
fnp-2749	61	4	.	.	X
fnp-2749	61	5	lebouvier	lebouvier	PROPN
fnp-2749	61	6	t	t	PROPN
fnp-2749	61	7	,	,	PUNCT
fnp-2749	61	8	tasselli	tasselli	PROPN
fnp-2749	61	9	m	m	PROPN
fnp-2749	61	10	,	,	PUNCT
fnp-2749	61	11	paillusson	paillusson	PROPN
fnp-2749	61	12	s	s	PROPN
fnp-2749	61	13	,	,	PUNCT
fnp-2749	61	14	pouclet	pouclet	ADJ
fnp-2749	61	15	h	h	NOUN
fnp-2749	61	16	,	,	PUNCT
fnp-2749	61	17	neunlist	neunlist	NOUN
fnp-2749	61	18	m	m	PROPN
fnp-2749	61	19	,	,	PUNCT
fnp-2749	61	20	derkinderen	derkinderen	PROPN
fnp-2749	61	21	p.	p.	PROPN
fnp-2749	61	22	biopsable	biopsable	ADJ
fnp-2749	61	23	neural	neural	ADJ
fnp-2749	61	24	tissues	tissue	NOUN
fnp-2749	61	25	:	:	PUNCT
fnp-2749	61	26	toward	toward	ADP
fnp-2749	61	27	new	new	ADJ
fnp-2749	61	28	biomarkers	biomarker	NOUN
fnp-2749	61	29	for	for	ADP
fnp-2749	61	30	parkinson	parkinson	NOUN
fnp-2749	61	31	’s	’s	PART
fnp-2749	61	32	disease	disease	NOUN
fnp-2749	61	33	?	?	PUNCT
fnp-2749	62	1	front	front	ADJ
fnp-2749	62	2	psychiatry	psychiatry	NOUN
fnp-2749	62	3	.	.	PUNCT
fnp-2749	63	1	2010;1:128	2010;1:128	NOUN
fnp-2749	63	2	.	.	PUNCT
fnp-2749	64	1	copyright	copyright	NOUN
fnp-2749	64	2	:	:	PUNCT
fnp-2749	64	3	©	©	PROPN
fnp-2749	64	4	2020	2020	NUM
fnp-2749	64	5	the	the	DET
fnp-2749	64	6	author(s	author(s	NOUN
fnp-2749	64	7	)	)	PUNCT
fnp-2749	64	8	.	.	PUNCT
fnp-2749	65	1	this	this	PRON
fnp-2749	65	2	is	be	AUX
fnp-2749	65	3	an	an	DET
fnp-2749	65	4	open	open	ADJ
fnp-2749	65	5	access	access	NOUN
fnp-2749	65	6	article	article	NOUN
fnp-2749	65	7	distributed	distribute	VERB
fnp-2749	65	8	under	under	ADP
fnp-2749	65	9	the	the	DET
fnp-2749	65	10	terms	term	NOUN
fnp-2749	65	11	of	of	ADP
fnp-2749	65	12	the	the	DET
fnp-2749	65	13	creative	creative	ADJ
fnp-2749	65	14	commons	common	NOUN
fnp-2749	65	15	attribution	attribution	NOUN
fnp-2749	65	16	4.0	4.0	NUM
fnp-2749	65	17	international	international	ADJ
fnp-2749	65	18	license	license	NOUN
fnp-2749	65	19	(	(	PUNCT
fnp-2749	65	20	https://creativecommons.org/licenses/by/4.0/	https://creativecommons.org/licenses/by/4.0/	PROPN
fnp-2749	65	21	)	)	PUNCT
fnp-2749	65	22	,	,	PUNCT
fnp-2749	65	23	which	which	PRON
fnp-2749	65	24	permits	permit	VERB
fnp-2749	65	25	unrestricted	unrestricted	ADJ
fnp-2749	65	26	use	use	NOUN
fnp-2749	65	27	,	,	PUNCT
fnp-2749	65	28	distribution	distribution	NOUN
fnp-2749	65	29	,	,	PUNCT
fnp-2749	65	30	and	and	CCONJ
fnp-2749	65	31	reproduction	reproduction	NOUN
fnp-2749	65	32	in	in	ADP
fnp-2749	65	33	any	any	DET
fnp-2749	65	34	medium	medium	NOUN
fnp-2749	65	35	,	,	PUNCT
fnp-2749	65	36	provided	provide	VERB
fnp-2749	65	37	the	the	DET
fnp-2749	65	38	original	original	ADJ
fnp-2749	65	39	author	author	NOUN
fnp-2749	65	40	and	and	CCONJ
fnp-2749	65	41	source	source	NOUN
fnp-2749	65	42	are	be	AUX
fnp-2749	65	43	credited	credit	VERB
fnp-2749	65	44	,	,	PUNCT
fnp-2749	65	45	a	a	DET
fnp-2749	65	46	link	link	NOUN
fnp-2749	65	47	to	to	ADP
fnp-2749	65	48	the	the	DET
fnp-2749	65	49	creative	creative	ADJ
fnp-2749	65	50	commons	common	NOUN
fnp-2749	65	51	license	license	NOUN
fnp-2749	65	52	is	be	AUX
fnp-2749	65	53	provided	provide	VERB
fnp-2749	65	54	,	,	PUNCT
fnp-2749	65	55	and	and	CCONJ
fnp-2749	65	56	any	any	DET
fnp-2749	65	57	changes	change	NOUN
fnp-2749	65	58	are	be	AUX
fnp-2749	65	59	indicated	indicate	VERB
fnp-2749	65	60	.	.	PUNCT
fnp-2749	66	1	the	the	DET
fnp-2749	66	2	creative	creative	ADJ
fnp-2749	66	3	commons	common	NOUN
fnp-2749	66	4	public	public	ADJ
fnp-2749	66	5	domain	domain	NOUN
fnp-2749	66	6	dedication	dedication	NOUN
fnp-2749	66	7	waiver	waiver	NOUN
fnp-2749	66	8	(	(	PUNCT
fnp-2749	66	9	https://creativecommons.org/publicdomain/zero/1.0/	https://creativecommons.org/publicdomain/zero/1.0/	PROPN
fnp-2749	66	10	)	)	PUNCT
fnp-2749	66	11	applies	apply	VERB
fnp-2749	66	12	to	to	ADP
fnp-2749	66	13	the	the	DET
fnp-2749	66	14	data	datum	NOUN
fnp-2749	66	15	made	make	VERB
fnp-2749	66	16	available	available	ADJ
fnp-2749	66	17	in	in	ADP
fnp-2749	66	18	this	this	DET
fnp-2749	66	19	article	article	NOUN
fnp-2749	66	20	,	,	PUNCT
fnp-2749	66	21	unless	unless	SCONJ
fnp-2749	66	22	otherwise	otherwise	ADV
fnp-2749	66	23	stated	state	VERB
fnp-2749	66	24	.	.	PUNCT