id	sid	tid	token	lemma	pos
fnp-5497	1	1	an	an	DET
fnp-5497	1	2	optimized	optimize	VERB
fnp-5497	1	3	filter	filter	NOUN
fnp-5497	1	4	trap	trap	NOUN
fnp-5497	1	5	assay	assay	NOUN
fnp-5497	1	6	for	for	ADP
fnp-5497	1	7	detecting	detect	VERB
fnp-5497	1	8	recombinant	recombinant	ADJ
fnp-5497	1	9	authentic	authentic	ADJ
fnp-5497	1	10	tau	tau	PROPN
fnp-5497	1	11	fibrils	fibril	NOUN
fnp-5497	1	12	feel	feel	VERB
fnp-5497	1	13	free	free	ADJ
fnp-5497	1	14	to	to	PART
fnp-5497	1	15	add	add	VERB
fnp-5497	1	16	comments	comment	NOUN
fnp-5497	1	17	by	by	ADP
fnp-5497	1	18	clicking	click	VERB
fnp-5497	1	19	these	these	DET
fnp-5497	1	20	icons	icon	NOUN
fnp-5497	1	21	on	on	ADP
fnp-5497	1	22	the	the	DET
fnp-5497	1	23	sidebar	sidebar	NOUN
fnp-5497	1	24	free	free	ADJ
fnp-5497	1	25	neuropathology	neuropathology	NOUN
fnp-5497	1	26	5:15	5:15	NUM
fnp-5497	1	27	(	(	PUNCT
fnp-5497	1	28	2024	2024	NUM
fnp-5497	1	29	)	)	PUNCT
fnp-5497	1	30	methods	method	NOUN
fnp-5497	1	31	an	an	DET
fnp-5497	1	32	optimized	optimize	VERB
fnp-5497	1	33	filter	filter	NOUN
fnp-5497	1	34	trap	trap	NOUN
fnp-5497	1	35	assay	assay	NOUN
fnp-5497	1	36	for	for	ADP
fnp-5497	1	37	detecting	detect	VERB
fnp-5497	1	38	recombinant	recombinant	ADJ
fnp-5497	1	39	authentic	authentic	ADJ
fnp-5497	1	40	tau	tau	PROPN
fnp-5497	1	41	fibrils	fibril	VERB
fnp-5497	1	42	allison	allison	PROPN
fnp-5497	1	43	r.	r.	PROPN
fnp-5497	1	44	balaj1	balaj1	PROPN
fnp-5497	1	45	,	,	PUNCT
fnp-5497	1	46	thomas	thomas	PROPN
fnp-5497	1	47	l.	l.	PROPN
fnp-5497	1	48	rothstein1	rothstein1	PROPN
fnp-5497	1	49	,	,	PUNCT
fnp-5497	1	50	hiroaki	hiroaki	PROPN
fnp-5497	1	51	kaku1	kaku1	PROPN
fnp-5497	1	52	department	department	PROPN
fnp-5497	1	53	of	of	ADP
fnp-5497	1	54	investigative	investigative	ADJ
fnp-5497	1	55	medicine	medicine	NOUN
fnp-5497	1	56	,	,	PUNCT
fnp-5497	2	1	western	western	PROPN
fnp-5497	2	2	michigan	michigan	PROPN
fnp-5497	2	3	university	university	PROPN
fnp-5497	2	4	homer	homer	NOUN
fnp-5497	2	5	stryker	stryker	PROPN
fnp-5497	2	6	m.d	m.d	PROPN
fnp-5497	2	7	.	.	PROPN
fnp-5497	2	8	school	school	NOUN
fnp-5497	2	9	of	of	ADP
fnp-5497	2	10	medicine	medicine	NOUN
fnp-5497	2	11	,	,	PUNCT
fnp-5497	2	12	kalamazoo	kalamazoo	PROPN
fnp-5497	2	13	,	,	PUNCT
fnp-5497	2	14	mi	mi	PROPN
fnp-5497	2	15	,	,	PUNCT
fnp-5497	2	16	usa	usa	PROPN
fnp-5497	2	17	corresponding	corresponding	PROPN
fnp-5497	2	18	author	author	NOUN
fnp-5497	2	19	:	:	PUNCT
fnp-5497	2	20	hiroaki	hiroaki	PROPN
fnp-5497	2	21	kaku	kaku	PROPN
fnp-5497	2	22	·	·	PUNCT
fnp-5497	2	23	department	department	PROPN
fnp-5497	2	24	of	of	ADP
fnp-5497	2	25	investigative	investigative	ADJ
fnp-5497	2	26	medicine	medicine	NOUN
fnp-5497	2	27	·	·	PUNCT
fnp-5497	2	28	western	western	ADJ
fnp-5497	2	29	michigan	michigan	PROPN
fnp-5497	2	30	university	university	PROPN
fnp-5497	2	31	homer	homer	NOUN
fnp-5497	2	32	stryker	stryker	PROPN
fnp-5497	2	33	m.d	m.d	PROPN
fnp-5497	2	34	.	.	PROPN
fnp-5497	2	35	school	school	NOUN
fnp-5497	2	36	of	of	ADP
fnp-5497	2	37	medicine	medicine	PROPN
fnp-5497	2	38	·	·	PUNCT
fnp-5497	2	39	kalamazoo	kalamazoo	PROPN
fnp-5497	2	40	,	,	PUNCT
fnp-5497	2	41	mi	mi	PROPN
fnp-5497	2	42	·	·	PROPN
fnp-5497	2	43	usa	usa	PROPN
fnp-5497	2	44	hiroaki.kaku@wmed.edu	hiroaki.kaku@wmed.edu	PROPN
fnp-5497	2	45	additional	additional	ADJ
fnp-5497	2	46	resources	resource	NOUN
fnp-5497	2	47	and	and	CCONJ
fnp-5497	2	48	electronic	electronic	ADJ
fnp-5497	2	49	supplementary	supplementary	ADJ
fnp-5497	2	50	material	material	NOUN
fnp-5497	2	51	:	:	PUNCT
fnp-5497	2	52	supplementary	supplementary	ADJ
fnp-5497	2	53	material	material	NOUN
fnp-5497	2	54	submitted	submit	VERB
fnp-5497	2	55	:	:	PUNCT
fnp-5497	2	56	23	23	NUM
fnp-5497	2	57	april	april	PROPN
fnp-5497	2	58	2024	2024	NUM
fnp-5497	2	59	accepted	accept	VERB
fnp-5497	2	60	:	:	PUNCT
fnp-5497	2	61	14	14	NUM
fnp-5497	2	62	july	july	PROPN
fnp-5497	2	63	2024	2024	NUM
fnp-5497	2	64	copyedited	copyedit	VERB
fnp-5497	2	65	by	by	ADP
fnp-5497	2	66	:	:	PUNCT
fnp-5497	2	67	georg	georg	NOUN
fnp-5497	2	68	haase	haase	NOUN
fnp-5497	2	69	published	publish	VERB
fnp-5497	2	70	:	:	PUNCT
fnp-5497	2	71	02	02	NUM
fnp-5497	2	72	august	august	PROPN
fnp-5497	2	73	2024	2024	NUM
fnp-5497	2	74	https://doi.org/10.17879/freeneuropathology-2024-5497	https://doi.org/10.17879/freeneuropathology-2024-5497	NOUN
fnp-5497	2	75	keywords	keyword	NOUN
fnp-5497	2	76	:	:	PUNCT
fnp-5497	2	77	truncated	truncate	VERB
fnp-5497	2	78	tau	tau	PROPN
fnp-5497	2	79	fibrils	fibril	NOUN
fnp-5497	2	80	,	,	PUNCT
fnp-5497	2	81	filter	filter	NOUN
fnp-5497	2	82	trap	trap	NOUN
fnp-5497	2	83	assay	assay	NOUN
fnp-5497	2	84	,	,	PUNCT
fnp-5497	2	85	sarkosyl	sarkosyl	NOUN
fnp-5497	2	86	,	,	PUNCT
fnp-5497	2	87	tauopathies	tauopathie	NOUN
fnp-5497	2	88	,	,	PUNCT
fnp-5497	2	89	protein	protein	NOUN
fnp-5497	2	90	aggregation	aggregation	NOUN
fnp-5497	2	91	abstract	abstract	VERB
fnp-5497	2	92	the	the	DET
fnp-5497	2	93	development	development	NOUN
fnp-5497	2	94	and	and	CCONJ
fnp-5497	2	95	optimization	optimization	NOUN
fnp-5497	2	96	of	of	ADP
fnp-5497	2	97	the	the	DET
fnp-5497	2	98	filter	filter	NOUN
fnp-5497	2	99	trap	trap	NOUN
fnp-5497	2	100	assay	assay	NOUN
fnp-5497	2	101	(	(	PUNCT
fnp-5497	2	102	fta	fta	PROPN
fnp-5497	2	103	)	)	PUNCT
fnp-5497	2	104	for	for	ADP
fnp-5497	2	105	the	the	DET
fnp-5497	2	106	detection	detection	NOUN
fnp-5497	2	107	of	of	ADP
fnp-5497	2	108	authentic	authentic	ADJ
fnp-5497	2	109	tau	tau	NOUN
fnp-5497	2	110	fibrils	fibril	NOUN
fnp-5497	2	111	in	in	ADP
fnp-5497	2	112	vitro	vitro	X
fnp-5497	2	113	mark	mark	VERB
fnp-5497	2	114	a	a	DET
fnp-5497	2	115	pivotal	pivotal	ADJ
fnp-5497	2	116	advancement	advancement	NOUN
fnp-5497	2	117	in	in	ADP
fnp-5497	2	118	the	the	DET
fnp-5497	2	119	realm	realm	NOUN
fnp-5497	2	120	of	of	ADP
fnp-5497	2	121	tauopathy	tauopathy	ADJ
fnp-5497	2	122	research	research	NOUN
fnp-5497	2	123	,	,	PUNCT
fnp-5497	2	124	particularly	particularly	ADV
fnp-5497	2	125	by	by	ADP
fnp-5497	2	126	addressing	address	VERB
fnp-5497	2	127	the	the	DET
fnp-5497	2	128	limitations	limitation	NOUN
fnp-5497	2	129	of	of	ADP
fnp-5497	2	130	using	use	VERB
fnp-5497	2	131	polyanion	polyanion	NOUN
fnp-5497	2	132	-	-	PUNCT
fnp-5497	2	133	induced	induce	VERB
fnp-5497	2	134	tau	tau	PROPN
fnp-5497	2	135	fibrils	fibril	NOUN
fnp-5497	2	136	,	,	PUNCT
fnp-5497	2	137	which	which	PRON
fnp-5497	2	138	structurally	structurally	ADV
fnp-5497	2	139	differ	differ	VERB
fnp-5497	2	140	from	from	ADP
fnp-5497	2	141	those	those	PRON
fnp-5497	2	142	isolated	isolate	VERB
fnp-5497	2	143	from	from	ADP
fnp-5497	2	144	tauopathy	tauopathy	ADJ
fnp-5497	2	145	patients	patient	NOUN
fnp-5497	2	146	.	.	PUNCT
fnp-5497	3	1	recently	recently	ADV
fnp-5497	3	2	it	it	PRON
fnp-5497	3	3	has	have	AUX
fnp-5497	3	4	been	be	AUX
fnp-5497	3	5	shown	show	VERB
fnp-5497	3	6	that	that	SCONJ
fnp-5497	3	7	truncated	truncate	VERB
fnp-5497	3	8	tau	tau	PROPN
fnp-5497	3	9	fragment	fragment	NOUN
fnp-5497	3	10	(	(	PUNCT
fnp-5497	3	11	297	297	NUM
fnp-5497	3	12	-	-	SYM
fnp-5497	3	13	391	391	NUM
fnp-5497	3	14	)	)	PUNCT
fnp-5497	3	15	,	,	PUNCT
fnp-5497	3	16	also	also	ADV
fnp-5497	3	17	termed	term	VERB
fnp-5497	3	18	dgae	dgae	NOUN
fnp-5497	3	19	,	,	PUNCT
fnp-5497	3	20	can	can	AUX
fnp-5497	3	21	form	form	VERB
fnp-5497	3	22	authentic	authentic	ADJ
fnp-5497	3	23	tau	tau	NOUN
fnp-5497	3	24	fibrils	fibril	NOUN
fnp-5497	3	25	in	in	ADP
fnp-5497	3	26	the	the	DET
fnp-5497	3	27	absence	absence	NOUN
fnp-5497	3	28	of	of	ADP
fnp-5497	3	29	polyanions	polyanion	NOUN
fnp-5497	3	30	.	.	PUNCT
fnp-5497	4	1	this	this	DET
fnp-5497	4	2	study	study	NOUN
fnp-5497	4	3	introduces	introduce	VERB
fnp-5497	4	4	a	a	DET
fnp-5497	4	5	refined	refined	ADJ
fnp-5497	4	6	protocol	protocol	NOUN
fnp-5497	4	7	that	that	PRON
fnp-5497	4	8	reliably	reliably	ADV
fnp-5497	4	9	detects	detect	VERB
fnp-5497	4	10	authentic	authentic	ADJ
fnp-5497	4	11	tau	tau	NOUN
fnp-5497	4	12	fibrils	fibril	VERB
fnp-5497	4	13	in	in	ADP
fnp-5497	4	14	a	a	DET
fnp-5497	4	15	physiologically	physiologically	ADV
fnp-5497	4	16	relevant	relevant	ADJ
fnp-5497	4	17	framework	framework	NOUN
fnp-5497	4	18	,	,	PUNCT
fnp-5497	4	19	utilizing	utilize	VERB
fnp-5497	4	20	nitrocellulose	nitrocellulose	NOUN
fnp-5497	4	21	membranes	membrane	NOUN
fnp-5497	4	22	to	to	PART
fnp-5497	4	23	achieve	achieve	VERB
fnp-5497	4	24	heightened	heightened	ADJ
fnp-5497	4	25	sensitivity	sensitivity	NOUN
fnp-5497	4	26	.	.	PUNCT
fnp-5497	5	1	our	our	PRON
fnp-5497	5	2	investigation	investigation	NOUN
fnp-5497	5	3	highlights	highlight	VERB
fnp-5497	5	4	the	the	DET
fnp-5497	5	5	superior	superior	ADJ
fnp-5497	5	6	efficacy	efficacy	NOUN
fnp-5497	5	7	of	of	ADP
fnp-5497	5	8	sarkosyl	sarkosyl	NOUN
fnp-5497	5	9	,	,	PUNCT
fnp-5497	5	10	an	an	DET
fnp-5497	5	11	anionic	anionic	ADJ
fnp-5497	5	12	surfactant	surfactant	NOUN
fnp-5497	5	13	traditionally	traditionally	ADV
fnp-5497	5	14	used	use	VERB
fnp-5497	5	15	to	to	PART
fnp-5497	5	16	prepare	prepare	VERB
fnp-5497	5	17	protein	protein	NOUN
fnp-5497	5	18	lysates	lysate	NOUN
fnp-5497	5	19	from	from	ADP
fnp-5497	5	20	brains	brain	NOUN
fnp-5497	5	21	and	and	CCONJ
fnp-5497	5	22	cultured	cultured	ADJ
fnp-5497	5	23	neurons	neuron	NOUN
fnp-5497	5	24	,	,	PUNCT
fnp-5497	5	25	in	in	ADP
fnp-5497	5	26	preserving	preserve	VERB
fnp-5497	5	27	the	the	DET
fnp-5497	5	28	aggregated	aggregated	ADJ
fnp-5497	5	29	state	state	NOUN
fnp-5497	5	30	of	of	ADP
fnp-5497	5	31	tau	tau	PROPN
fnp-5497	5	32	dgae	dgae	NOUN
fnp-5497	5	33	fibrils	fibril	NOUN
fnp-5497	5	34	in	in	ADP
fnp-5497	5	35	vitro	vitro	NOUN
fnp-5497	5	36	,	,	PUNCT
fnp-5497	5	37	underscoring	underscore	VERB
fnp-5497	5	38	its	its	PRON
fnp-5497	5	39	potential	potential	NOUN
fnp-5497	5	40	for	for	ADP
fnp-5497	5	41	further	further	ADJ
fnp-5497	5	42	exploratory	exploratory	ADJ
fnp-5497	5	43	studies	study	NOUN
fnp-5497	5	44	.	.	PUNCT
fnp-5497	6	1	by	by	ADP
fnp-5497	6	2	offering	offer	VERB
fnp-5497	6	3	a	a	DET
fnp-5497	6	4	user	user	NOUN
fnp-5497	6	5	-	-	PUNCT
fnp-5497	6	6	friendly	friendly	ADJ
fnp-5497	6	7	and	and	CCONJ
fnp-5497	6	8	economically	economically	ADV
fnp-5497	6	9	feasible	feasible	ADJ
fnp-5497	6	10	approach	approach	NOUN
fnp-5497	6	11	,	,	PUNCT
fnp-5497	6	12	this	this	DET
fnp-5497	6	13	technique	technique	NOUN
fnp-5497	6	14	enables	enable	VERB
fnp-5497	6	15	a	a	DET
fnp-5497	6	16	broad	broad	ADJ
fnp-5497	6	17	range	range	NOUN
fnp-5497	6	18	of	of	ADP
fnp-5497	6	19	laboratories	laboratory	NOUN
fnp-5497	6	20	to	to	PART
fnp-5497	6	21	measure	measure	VERB
fnp-5497	6	22	the	the	DET
fnp-5497	6	23	presence	presence	NOUN
fnp-5497	6	24	of	of	ADP
fnp-5497	6	25	authentic	authentic	ADJ
fnp-5497	6	26	tau	tau	PROPN
fnp-5497	6	27	fibrils	fibril	NOUN
fnp-5497	6	28	.	.	PUNCT
fnp-5497	7	1	this	this	DET
fnp-5497	7	2	methodological	methodological	ADJ
fnp-5497	7	3	enhancement	enhancement	NOUN
fnp-5497	7	4	propels	propel	VERB
fnp-5497	7	5	our	our	PRON
fnp-5497	7	6	understanding	understanding	NOUN
fnp-5497	7	7	of	of	ADP
fnp-5497	7	8	tauopathies	tauopathie	NOUN
fnp-5497	7	9	forward	forward	ADV
fnp-5497	7	10	and	and	CCONJ
fnp-5497	7	11	bridges	bridge	VERB
fnp-5497	7	12	the	the	DET
fnp-5497	7	13	gap	gap	NOUN
fnp-5497	7	14	between	between	ADP
fnp-5497	7	15	basic	basic	ADJ
fnp-5497	7	16	research	research	NOUN
fnp-5497	7	17	and	and	CCONJ
fnp-5497	7	18	advanced	advanced	ADJ
fnp-5497	7	19	structural	structural	ADJ
fnp-5497	7	20	analyses	analysis	NOUN
fnp-5497	7	21	,	,	PUNCT
fnp-5497	7	22	enriching	enrich	VERB
fnp-5497	7	23	the	the	DET
fnp-5497	7	24	scientific	scientific	ADJ
fnp-5497	7	25	community	community	NOUN
fnp-5497	7	26	's	's	PART
fnp-5497	7	27	methodologies	methodology	NOUN
fnp-5497	7	28	for	for	ADP
fnp-5497	7	29	studying	study	VERB
fnp-5497	7	30	neurodegenerative	neurodegenerative	ADJ
fnp-5497	7	31	disorders	disorder	NOUN
fnp-5497	7	32	.	.	PUNCT
fnp-5497	8	1	introduction	introduction	NOUN
fnp-5497	8	2	the	the	DET
fnp-5497	8	3	microtubule	microtubule	NOUN
fnp-5497	8	4	-	-	PUNCT
fnp-5497	8	5	associated	associate	VERB
fnp-5497	8	6	protein	protein	NOUN
fnp-5497	8	7	tau	tau	PROPN
fnp-5497	8	8	forms	form	VERB
fnp-5497	8	9	disease	disease	NOUN
fnp-5497	8	10	-	-	PUNCT
fnp-5497	8	11	specific	specific	ADJ
fnp-5497	8	12	filamentous	filamentous	ADJ
fnp-5497	8	13	aggregates	aggregate	NOUN
fnp-5497	8	14	in	in	ADP
fnp-5497	8	15	tauopathies	tauopathie	NOUN
fnp-5497	8	16	,	,	PUNCT
fnp-5497	8	17	including	include	VERB
fnp-5497	8	18	alzheimer	alzheimer	PROPN
fnp-5497	8	19	’s	’s	PART
fnp-5497	8	20	diseases	disease	NOUN
fnp-5497	8	21	(	(	PUNCT
fnp-5497	8	22	ad	ad	NOUN
fnp-5497	8	23	)	)	PUNCT
fnp-5497	8	24	.	.	PUNCT
fnp-5497	9	1	recent	recent	ADJ
fnp-5497	9	2	advances	advance	NOUN
fnp-5497	9	3	in	in	ADP
fnp-5497	9	4	cryo	cryo	NOUN
fnp-5497	9	5	-	-	PUNCT
fnp-5497	9	6	electron	electron	NOUN
fnp-5497	9	7	microscopy	microscopy	NOUN
fnp-5497	9	8	(	(	PUNCT
fnp-5497	9	9	cryo	cryo	NOUN
fnp-5497	9	10	-	-	PUNCT
fnp-5497	9	11	em	em	PROPN
fnp-5497	9	12	)	)	PUNCT
fnp-5497	9	13	have	have	AUX
fnp-5497	9	14	made	make	VERB
fnp-5497	9	15	it	it	PRON
fnp-5497	9	16	a	a	DET
fnp-5497	9	17	powerful	powerful	ADJ
fnp-5497	9	18	tool	tool	NOUN
fnp-5497	9	19	for	for	ADP
fnp-5497	9	20	determining	determine	VERB
fnp-5497	9	21	the	the	DET
fnp-5497	9	22	3d	3d	NUM
fnp-5497	9	23	structure	structure	NOUN
fnp-5497	9	24	of	of	ADP
fnp-5497	9	25	various	various	ADJ
fnp-5497	9	26	proteins	protein	NOUN
fnp-5497	9	27	,	,	PUNCT
fnp-5497	9	28	including	include	VERB
fnp-5497	9	29	tau	tau	PROPN
fnp-5497	9	30	aggregates	aggregate	NOUN
fnp-5497	9	31	.	.	PUNCT
fnp-5497	10	1	the	the	DET
fnp-5497	10	2	structure	structure	NOUN
fnp-5497	10	3	of	of	ADP
fnp-5497	10	4	aggregated	aggregate	VERB
fnp-5497	10	5	tau	tau	PROPN
fnp-5497	10	6	from	from	ADP
fnp-5497	10	7	ad	ad	NOUN
fnp-5497	10	8	patients	patient	NOUN
fnp-5497	10	9	was	be	AUX
fnp-5497	10	10	determined	determine	VERB
fnp-5497	10	11	:	:	PUNCT
fnp-5497	10	12	tau	tau	PROPN
fnp-5497	10	13	filament	filament	NOUN
fnp-5497	10	14	cores	core	NOUN
fnp-5497	10	15	are	be	AUX
fnp-5497	10	16	made	make	VERB
fnp-5497	10	17	of	of	ADP
fnp-5497	10	18	two	two	NUM
fnp-5497	10	19	identical	identical	ADJ
fnp-5497	10	20	protofilaments	protofilament	NOUN
fnp-5497	10	21	comprising	comprise	VERB
fnp-5497	10	22	residues	residue	NOUN
fnp-5497	10	23	306	306	NUM
fnp-5497	10	24	-	-	SYM
fnp-5497	10	25	378	378	NUM
fnp-5497	10	26	of	of	ADP
fnp-5497	10	27	tau	tau	PROPN
fnp-5497	10	28	,	,	PUNCT
fnp-5497	10	29	which	which	PRON
fnp-5497	10	30	adopt	adopt	VERB
fnp-5497	10	31	a	a	DET
fnp-5497	10	32	combined	combine	VERB
fnp-5497	10	33	cross	cross	NOUN
fnp-5497	10	34	-	-	NOUN
fnp-5497	10	35	β	β	ADJ
fnp-5497	10	36	/	/	SYM
fnp-5497	10	37	β	β	NOUN
fnp-5497	10	38	-	-	PUNCT
fnp-5497	10	39	helix	helix	ADJ
fnp-5497	10	40	structure	structure	NOUN
fnp-5497	10	41	and	and	CCONJ
fnp-5497	10	42	define	define	VERB
fnp-5497	10	43	the	the	DET
fnp-5497	10	44	seed	seed	NOUN
fnp-5497	10	45	for	for	ADP
fnp-5497	10	46	tau	tau	PROPN
fnp-5497	10	47	aggregation1	aggregation1	PROPN
fnp-5497	10	48	.	.	PUNCT
fnp-5497	11	1	to	to	PART
fnp-5497	11	2	model	model	VERB
fnp-5497	11	3	tau	tau	PROPN
fnp-5497	11	4	aggregation	aggregation	NOUN
fnp-5497	11	5	in	in	ADP
fnp-5497	11	6	vitro	vitro	X
fnp-5497	11	7	,	,	PUNCT
fnp-5497	11	8	polyanions	polyanion	NOUN
fnp-5497	11	9	,	,	PUNCT
fnp-5497	11	10	such	such	ADJ
fnp-5497	11	11	as	as	ADP
fnp-5497	11	12	heparin	heparin	NOUN
fnp-5497	11	13	,	,	PUNCT
fnp-5497	11	14	have	have	AUX
fnp-5497	11	15	been	be	AUX
fnp-5497	11	16	widely	widely	ADV
fnp-5497	11	17	used	use	VERB
fnp-5497	11	18	to	to	PART
fnp-5497	11	19	mimic	mimic	VERB
fnp-5497	11	20	tau	tau	PROPN
fnp-5497	11	21	aggregation2,3	aggregation2,3	PROPN
fnp-5497	11	22	.	.	PUNCT
fnp-5497	12	1	however	however	ADV
fnp-5497	12	2	,	,	PUNCT
fnp-5497	12	3	the	the	DET
fnp-5497	12	4	3d	3d	NOUN
fnp-5497	12	5	structure	structure	NOUN
fnp-5497	12	6	of	of	ADP
fnp-5497	12	7	heparin	heparin	NOUN
fnp-5497	12	8	-	-	PUNCT
fnp-5497	12	9	induced	induce	VERB
fnp-5497	12	10	tau	tau	NOUN
fnp-5497	12	11	fibrils	fibril	NOUN
fnp-5497	12	12	is	be	AUX
fnp-5497	12	13	quite	quite	ADV
fnp-5497	12	14	different	different	ADJ
fnp-5497	12	15	from	from	ADP
fnp-5497	12	16	those	those	PRON
fnp-5497	12	17	found	find	VERB
fnp-5497	12	18	in	in	ADP
fnp-5497	12	19	patients	patient	NOUN
fnp-5497	12	20	.	.	PUNCT
fnp-5497	13	1	unlike	unlike	ADP
fnp-5497	13	2	the	the	DET
fnp-5497	13	3	uniform	uniform	ADJ
fnp-5497	13	4	structure	structure	NOUN
fnp-5497	13	5	observed	observe	VERB
fnp-5497	13	6	in	in	ADP
fnp-5497	13	7	disease	disease	NOUN
fnp-5497	13	8	-	-	PUNCT
fnp-5497	13	9	related	relate	VERB
fnp-5497	13	10	tau	tau	NOUN
fnp-5497	13	11	,	,	PUNCT
fnp-5497	13	12	heparin	heparin	NOUN
fnp-5497	13	13	-	-	PUNCT
fnp-5497	13	14	induced	induce	VERB
fnp-5497	13	15	tau	tau	PROPN
fnp-5497	13	16	fibrils	fibril	NOUN
fnp-5497	13	17	are	be	AUX
fnp-5497	13	18	a	a	DET
fnp-5497	13	19	mixture	mixture	NOUN
fnp-5497	13	20	of	of	ADP
fnp-5497	13	21	three	three	NUM
fnp-5497	13	22	distinct	distinct	ADJ
fnp-5497	13	23	structures	structure	NOUN
fnp-5497	13	24	:	:	PUNCT
fnp-5497	13	25	snake	snake	NOUN
fnp-5497	13	26	,	,	PUNCT
fnp-5497	13	27	twister	twister	NOUN
fnp-5497	13	28	,	,	PUNCT
fnp-5497	13	29	and	and	CCONJ
fnp-5497	13	30	jagged4	jagged4	X
fnp-5497	13	31	.	.	PUNCT
fnp-5497	14	1	thus	thus	ADV
fnp-5497	14	2	,	,	PUNCT
fnp-5497	14	3	heparin	heparin	NOUN
fnp-5497	14	4	-	-	PUNCT
fnp-5497	14	5	induced	induce	VERB
fnp-5497	14	6	tau	tau	PROPN
fnp-5497	14	7	aggregates	aggregate	NOUN
fnp-5497	14	8	may	may	AUX
fnp-5497	14	9	not	not	PART
fnp-5497	14	10	represent	represent	VERB
fnp-5497	14	11	a	a	DET
fnp-5497	14	12	physiological	physiological	ADJ
fnp-5497	14	13	model	model	NOUN
fnp-5497	14	14	for	for	ADP
fnp-5497	14	15	in	in	ADP
fnp-5497	14	16	vitro	vitro	X
fnp-5497	14	17	studies	study	NOUN
fnp-5497	14	18	.	.	PUNCT
fnp-5497	15	1	this	this	PRON
fnp-5497	15	2	raises	raise	VERB
fnp-5497	15	3	important	important	ADJ
fnp-5497	15	4	considerations	consideration	NOUN
fnp-5497	15	5	about	about	ADP
fnp-5497	15	6	the	the	DET
fnp-5497	15	7	relevance	relevance	NOUN
fnp-5497	15	8	and	and	CCONJ
fnp-5497	15	9	accuracy	accuracy	NOUN
fnp-5497	15	10	of	of	ADP
fnp-5497	15	11	using	use	VERB
fnp-5497	15	12	heparin	heparin	NOUN
fnp-5497	15	13	-	-	PUNCT
fnp-5497	15	14	induced	induce	VERB
fnp-5497	15	15	models	model	NOUN
fnp-5497	15	16	in	in	ADP
fnp-5497	15	17	tau	tau	PROPN
fnp-5497	15	18	aggregation	aggregation	NOUN
fnp-5497	15	19	research	research	NOUN
fnp-5497	15	20	.	.	PUNCT
fnp-5497	16	1	to	to	PART
fnp-5497	16	2	overcome	overcome	VERB
fnp-5497	16	3	this	this	DET
fnp-5497	16	4	issue	issue	NOUN
fnp-5497	16	5	,	,	PUNCT
fnp-5497	16	6	methods	method	NOUN
fnp-5497	16	7	to	to	PART
fnp-5497	16	8	generate	generate	VERB
fnp-5497	16	9	tau	tau	PROPN
fnp-5497	16	10	aggregates	aggregate	NOUN
fnp-5497	16	11	without	without	ADP
fnp-5497	16	12	the	the	DET
fnp-5497	16	13	need	need	NOUN
fnp-5497	16	14	for	for	SCONJ
fnp-5497	16	15	polyanions	polyanion	NOUN
fnp-5497	16	16	were	be	AUX
fnp-5497	16	17	identified	identify	VERB
fnp-5497	16	18	using	use	VERB
fnp-5497	16	19	the	the	DET
fnp-5497	16	20	aggregation	aggregation	NOUN
fnp-5497	16	21	core	core	NOUN
fnp-5497	16	22	region	region	NOUN
fnp-5497	16	23	,	,	PUNCT
fnp-5497	16	24	such	such	ADJ
fnp-5497	16	25	as	as	ADP
fnp-5497	16	26	truncated	truncated	ADJ
fnp-5497	16	27	tau	tau	PROPN
fnp-5497	16	28	297	297	NUM
fnp-5497	16	29	-	-	SYM
fnp-5497	16	30	391	391	NUM
fnp-5497	16	31	,	,	PUNCT
fnp-5497	16	32	termed	term	VERB
fnp-5497	16	33	dgae	dgae	NOUN
fnp-5497	16	34	tau5,6	tau5,6	PRON
fnp-5497	16	35	.	.	PUNCT
fnp-5497	17	1	this	this	DET
fnp-5497	17	2	breakthrough	breakthrough	NOUN
fnp-5497	17	3	offers	offer	VERB
fnp-5497	17	4	new	new	ADJ
fnp-5497	17	5	avenues	avenue	NOUN
fnp-5497	17	6	for	for	ADP
fnp-5497	17	7	studying	study	VERB
fnp-5497	17	8	tau	tau	PROPN
fnp-5497	17	9	aggregation	aggregation	NOUN
fnp-5497	17	10	more	more	ADV
fnp-5497	17	11	physiologically	physiologically	ADV
fnp-5497	17	12	.	.	PUNCT
fnp-5497	18	1	the	the	DET
fnp-5497	18	2	filter	filter	NOUN
fnp-5497	18	3	trap	trap	NOUN
fnp-5497	18	4	assay	assay	NOUN
fnp-5497	18	5	(	(	PUNCT
fnp-5497	18	6	fta	fta	PROPN
fnp-5497	18	7	)	)	PUNCT
fnp-5497	18	8	,	,	PUNCT
fnp-5497	18	9	also	also	ADV
fnp-5497	18	10	known	know	VERB
fnp-5497	18	11	as	as	ADP
fnp-5497	18	12	the	the	DET
fnp-5497	18	13	filter	filter	NOUN
fnp-5497	18	14	retardation	retardation	NOUN
fnp-5497	18	15	assay	assay	NOUN
fnp-5497	18	16	,	,	PUNCT
fnp-5497	18	17	is	be	AUX
fnp-5497	18	18	a	a	DET
fnp-5497	18	19	widely	widely	ADV
fnp-5497	18	20	recognized	recognize	VERB
fnp-5497	18	21	,	,	PUNCT
fnp-5497	18	22	user	user	NOUN
fnp-5497	18	23	-	-	PUNCT
fnp-5497	18	24	friendly	friendly	ADJ
fnp-5497	18	25	in	in	ADP
fnp-5497	18	26	vitro	vitro	X
fnp-5497	18	27	method	method	NOUN
fnp-5497	18	28	for	for	ADP
fnp-5497	18	29	detecting	detect	VERB
fnp-5497	18	30	protein	protein	NOUN
fnp-5497	18	31	aggregates	aggregate	NOUN
fnp-5497	18	32	including	include	VERB
fnp-5497	18	33	polyanion	polyanion	NOUN
fnp-5497	18	34	-	-	PUNCT
fnp-5497	18	35	induced	induce	VERB
fnp-5497	18	36	tau	tau	PROPN
fnp-5497	18	37	aggregates	aggregate	NOUN
fnp-5497	18	38	.	.	PUNCT
fnp-5497	19	1	given	give	VERB
fnp-5497	19	2	the	the	DET
fnp-5497	19	3	structural	structural	ADJ
fnp-5497	19	4	differences	difference	NOUN
fnp-5497	19	5	between	between	ADP
fnp-5497	19	6	polyanion	polyanion	NOUN
fnp-5497	19	7	-	-	PUNCT
fnp-5497	19	8	induced	induce	VERB
fnp-5497	19	9	tau	tau	NOUN
fnp-5497	19	10	aggregates	aggregate	NOUN
fnp-5497	19	11	and	and	CCONJ
fnp-5497	19	12	those	those	PRON
fnp-5497	19	13	extracted	extract	VERB
fnp-5497	19	14	from	from	ADP
fnp-5497	19	15	tauopathy	tauopathy	ADJ
fnp-5497	19	16	patients	patient	NOUN
fnp-5497	19	17	,	,	PUNCT
fnp-5497	19	18	work	work	VERB
fnp-5497	19	19	on	on	ADP
fnp-5497	19	20	the	the	DET
fnp-5497	19	21	aggregation	aggregation	NOUN
fnp-5497	19	22	of	of	ADP
fnp-5497	19	23	the	the	DET
fnp-5497	19	24	dgae	dgae	NOUN
fnp-5497	19	25	tau	tau	PROPN
fnp-5497	19	26	variant	variant	NOUN
fnp-5497	19	27	is	be	AUX
fnp-5497	19	28	expected	expect	VERB
fnp-5497	19	29	to	to	PART
fnp-5497	19	30	increase	increase	VERB
fnp-5497	19	31	.	.	PUNCT
fnp-5497	20	1	a	a	DET
fnp-5497	20	2	common	common	ADJ
fnp-5497	20	3	approach	approach	NOUN
fnp-5497	20	4	to	to	PART
fnp-5497	20	5	assess	assess	VERB
fnp-5497	20	6	dgae	dgae	NOUN
fnp-5497	20	7	tau	tau	PROPN
fnp-5497	20	8	aggregation	aggregation	NOUN
fnp-5497	20	9	involves	involve	VERB
fnp-5497	20	10	the	the	DET
fnp-5497	20	11	use	use	NOUN
fnp-5497	20	12	of	of	ADP
fnp-5497	20	13	thioflavin	thioflavin	PROPN
fnp-5497	20	14	t	t	PROPN
fnp-5497	20	15	(	(	PUNCT
fnp-5497	20	16	tht	tht	NOUN
fnp-5497	20	17	)	)	PUNCT
fnp-5497	20	18	fluorescence	fluorescence	NOUN
fnp-5497	20	19	assays	assay	NOUN
fnp-5497	20	20	.	.	PUNCT
fnp-5497	21	1	however	however	ADV
fnp-5497	21	2	,	,	PUNCT
fnp-5497	21	3	these	these	DET
fnp-5497	21	4	fluorescence	fluorescence	NOUN
fnp-5497	21	5	-	-	PUNCT
fnp-5497	21	6	based	base	VERB
fnp-5497	21	7	assays	assay	NOUN
fnp-5497	21	8	sometimes	sometimes	ADV
fnp-5497	21	9	yield	yield	VERB
fnp-5497	21	10	inconsistent	inconsistent	ADJ
fnp-5497	21	11	results	result	NOUN
fnp-5497	21	12	,	,	PUNCT
fnp-5497	21	13	necessitating	necessitate	VERB
fnp-5497	21	14	alternative	alternative	ADJ
fnp-5497	21	15	methods	method	NOUN
fnp-5497	21	16	to	to	PART
fnp-5497	21	17	verify	verify	VERB
fnp-5497	21	18	findings	finding	NOUN
fnp-5497	21	19	.	.	PUNCT
fnp-5497	22	1	in	in	ADP
fnp-5497	22	2	this	this	DET
fnp-5497	22	3	context	context	NOUN
fnp-5497	22	4	,	,	PUNCT
fnp-5497	22	5	fta	fta	PROPN
fnp-5497	22	6	,	,	PUNCT
fnp-5497	22	7	which	which	PRON
fnp-5497	22	8	utilizes	utilize	VERB
fnp-5497	22	9	a	a	DET
fnp-5497	22	10	distinct	distinct	ADJ
fnp-5497	22	11	mechanism	mechanism	NOUN
fnp-5497	22	12	for	for	ADP
fnp-5497	22	13	detecting	detect	VERB
fnp-5497	22	14	tau	tau	PROPN
fnp-5497	22	15	aggregates	aggregate	NOUN
fnp-5497	22	16	,	,	PUNCT
fnp-5497	22	17	emerges	emerge	VERB
fnp-5497	22	18	as	as	ADP
fnp-5497	22	19	an	an	DET
fnp-5497	22	20	essential	essential	ADJ
fnp-5497	22	21	complementary	complementary	ADJ
fnp-5497	22	22	technique	technique	NOUN
fnp-5497	22	23	.	.	PUNCT
fnp-5497	23	1	while	while	SCONJ
fnp-5497	23	2	fta	fta	PROPN
fnp-5497	23	3	detection	detection	NOUN
fnp-5497	23	4	methods	method	NOUN
fnp-5497	23	5	for	for	ADP
fnp-5497	23	6	polyanion	polyanion	NOUN
fnp-5497	23	7	-	-	PUNCT
fnp-5497	23	8	induced	induce	VERB
fnp-5497	23	9	tau	tau	PROPN
fnp-5497	23	10	aggregates	aggregate	NOUN
fnp-5497	23	11	have	have	AUX
fnp-5497	23	12	been	be	AUX
fnp-5497	23	13	previously	previously	ADV
fnp-5497	23	14	established	establish	VERB
fnp-5497	23	15	,	,	PUNCT
fnp-5497	23	16	the	the	DET
fnp-5497	23	17	methods	method	NOUN
fnp-5497	23	18	for	for	ADP
fnp-5497	23	19	detecting	detect	VERB
fnp-5497	23	20	authentic	authentic	ADJ
fnp-5497	23	21	tau	tau	PROPN
fnp-5497	23	22	fibrils	fibril	NOUN
fnp-5497	23	23	remain	remain	VERB
fnp-5497	23	24	largely	largely	ADV
fnp-5497	23	25	unexplored7	unexplored7	ADJ
fnp-5497	23	26	.	.	PUNCT
fnp-5497	24	1	the	the	DET
fnp-5497	24	2	notable	notable	ADJ
fnp-5497	24	3	structural	structural	ADJ
fnp-5497	24	4	differences	difference	NOUN
fnp-5497	24	5	between	between	ADP
fnp-5497	24	6	these	these	DET
fnp-5497	24	7	fibrils	fibril	NOUN
fnp-5497	24	8	suggest	suggest	VERB
fnp-5497	24	9	distinct	distinct	ADJ
fnp-5497	24	10	biochemical	biochemical	ADJ
fnp-5497	24	11	characteristics	characteristic	NOUN
fnp-5497	24	12	.	.	PUNCT
fnp-5497	25	1	herein	herein	NOUN
fnp-5497	25	2	,	,	PUNCT
fnp-5497	25	3	we	we	PRON
fnp-5497	25	4	present	present	VERB
fnp-5497	25	5	an	an	DET
fnp-5497	25	6	optimized	optimize	VERB
fnp-5497	25	7	fta	fta	NOUN
fnp-5497	25	8	protocol	protocol	NOUN
fnp-5497	25	9	for	for	ADP
fnp-5497	25	10	analyzing	analyze	VERB
fnp-5497	25	11	dgae	dgae	NOUN
fnp-5497	25	12	tau	tau	PROPN
fnp-5497	25	13	aggregates	aggregate	NOUN
fnp-5497	25	14	.	.	PUNCT
fnp-5497	26	1	this	this	DET
fnp-5497	26	2	protocol	protocol	NOUN
fnp-5497	26	3	is	be	AUX
fnp-5497	26	4	designed	design	VERB
fnp-5497	26	5	to	to	PART
fnp-5497	26	6	provide	provide	VERB
fnp-5497	26	7	more	more	ADV
fnp-5497	26	8	reliable	reliable	ADJ
fnp-5497	26	9	and	and	CCONJ
fnp-5497	26	10	reproducible	reproducible	ADJ
fnp-5497	26	11	results	result	NOUN
fnp-5497	26	12	than	than	ADP
fnp-5497	26	13	previous	previous	ADJ
fnp-5497	26	14	protocols	protocol	NOUN
fnp-5497	26	15	,	,	PUNCT
fnp-5497	26	16	thereby	thereby	ADV
fnp-5497	26	17	enhancing	enhance	VERB
fnp-5497	26	18	the	the	DET
fnp-5497	26	19	study	study	NOUN
fnp-5497	26	20	of	of	ADP
fnp-5497	26	21	tau	tau	PROPN
fnp-5497	26	22	aggregation	aggregation	NOUN
fnp-5497	26	23	dynamics	dynamic	NOUN
fnp-5497	26	24	in	in	ADP
fnp-5497	26	25	vitro	vitro	X
fnp-5497	26	26	and	and	CCONJ
fnp-5497	26	27	contributing	contribute	VERB
fnp-5497	26	28	to	to	ADP
fnp-5497	26	29	a	a	DET
fnp-5497	26	30	deeper	deep	ADJ
fnp-5497	26	31	understanding	understanding	NOUN
fnp-5497	26	32	of	of	ADP
fnp-5497	26	33	tauopathies	tauopathie	NOUN
fnp-5497	26	34	.	.	PUNCT
fnp-5497	27	1	results	result	NOUN
fnp-5497	27	2	initially	initially	ADV
fnp-5497	27	3	,	,	PUNCT
fnp-5497	27	4	we	we	PRON
fnp-5497	27	5	followed	follow	VERB
fnp-5497	27	6	the	the	DET
fnp-5497	27	7	fta	fta	PROPN
fnp-5497	27	8	protocol	protocol	NOUN
fnp-5497	27	9	published	publish	VERB
fnp-5497	27	10	in	in	ADP
fnp-5497	27	11	20087	20087	NUM
fnp-5497	27	12	.	.	PUNCT
fnp-5497	28	1	according	accord	VERB
fnp-5497	28	2	to	to	ADP
fnp-5497	28	3	this	this	DET
fnp-5497	28	4	protocol	protocol	NOUN
fnp-5497	28	5	,	,	PUNCT
fnp-5497	28	6	tau	tau	PROPN
fnp-5497	28	7	protein	protein	NOUN
fnp-5497	28	8	samples	sample	NOUN
fnp-5497	28	9	are	be	AUX
fnp-5497	28	10	diluted	dilute	VERB
fnp-5497	28	11	in	in	ADP
fnp-5497	28	12	2	2	NUM
fnp-5497	28	13	 	 	SPACE
fnp-5497	28	14	%	%	NOUN
fnp-5497	28	15	(	(	PUNCT
fnp-5497	28	16	w	w	NOUN
fnp-5497	28	17	/	/	SYM
fnp-5497	28	18	v	v	NOUN
fnp-5497	28	19	)	)	PUNCT
fnp-5497	28	20	sodium	sodium	NOUN
fnp-5497	28	21	dodecyl	dodecyl	NOUN
fnp-5497	28	22	sulfate	sulfate	NOUN
fnp-5497	28	23	(	(	PUNCT
fnp-5497	28	24	sds	sds	PROPN
fnp-5497	28	25	)	)	PUNCT
fnp-5497	28	26	and	and	CCONJ
fnp-5497	28	27	the	the	DET
fnp-5497	28	28	fta	fta	PROPN
fnp-5497	28	29	is	be	AUX
fnp-5497	28	30	conducted	conduct	VERB
fnp-5497	28	31	using	use	VERB
fnp-5497	28	32	three	three	NUM
fnp-5497	28	33	types	type	NOUN
fnp-5497	28	34	of	of	ADP
fnp-5497	28	35	membranes	membrane	NOUN
fnp-5497	28	36	:	:	PUNCT
fnp-5497	28	37	nitrocellulose	nitrocellulose	NOUN
fnp-5497	28	38	,	,	PUNCT
fnp-5497	28	39	cellulose	cellulose	NOUN
fnp-5497	28	40	acetate	acetate	NOUN
fnp-5497	28	41	,	,	PUNCT
fnp-5497	28	42	and	and	CCONJ
fnp-5497	28	43	polyvinylidene	polyvinylidene	NOUN
fnp-5497	28	44	fluoride	fluoride	NOUN
fnp-5497	28	45	(	(	PUNCT
fnp-5497	28	46	pvdf)7	pvdf)7	PROPN
fnp-5497	28	47	.	.	PUNCT
fnp-5497	29	1	our	our	PRON
fnp-5497	29	2	findings	finding	NOUN
fnp-5497	29	3	indicated	indicate	VERB
fnp-5497	29	4	that	that	SCONJ
fnp-5497	29	5	the	the	DET
fnp-5497	29	6	nitrocellulose	nitrocellulose	NOUN
fnp-5497	29	7	membrane	membrane	NOUN
fnp-5497	29	8	yielded	yield	VERB
fnp-5497	29	9	the	the	DET
fnp-5497	29	10	highest	high	ADJ
fnp-5497	29	11	sensitivity	sensitivity	NOUN
fnp-5497	29	12	.	.	PUNCT
fnp-5497	30	1	notably	notably	ADV
fnp-5497	30	2	,	,	PUNCT
fnp-5497	30	3	the	the	DET
fnp-5497	30	4	sensitivity	sensitivity	NOUN
fnp-5497	30	5	of	of	ADP
fnp-5497	30	6	dgae	dgae	NOUN
fnp-5497	30	7	tau	tau	PROPN
fnp-5497	30	8	fibrils	fibril	VERB
fnp-5497	30	9	in	in	ADP
fnp-5497	30	10	the	the	DET
fnp-5497	30	11	fta	fta	NOUN
fnp-5497	30	12	diminished	diminish	VERB
fnp-5497	30	13	when	when	SCONJ
fnp-5497	30	14	diluted	dilute	VERB
fnp-5497	30	15	in	in	ADP
fnp-5497	30	16	2	2	NUM
fnp-5497	30	17	 	 	SPACE
fnp-5497	30	18	%	%	NOUN
fnp-5497	30	19	sds	sds	NOUN
fnp-5497	30	20	(	(	PUNCT
fnp-5497	30	21	figure	figure	NOUN
fnp-5497	30	22	 	 	SPACE
fnp-5497	30	23	1a	1a	NOUN
fnp-5497	30	24	)	)	PUNCT
fnp-5497	30	25	.	.	PUNCT
fnp-5497	31	1	subsequently	subsequently	ADV
fnp-5497	31	2	,	,	PUNCT
fnp-5497	31	3	we	we	PRON
fnp-5497	31	4	explored	explore	VERB
fnp-5497	31	5	fta	fta	NOUN
fnp-5497	31	6	using	use	VERB
fnp-5497	31	7	various	various	ADJ
fnp-5497	31	8	solutions	solution	NOUN
fnp-5497	31	9	,	,	PUNCT
fnp-5497	31	10	such	such	ADJ
fnp-5497	31	11	as	as	ADP
fnp-5497	31	12	phosphate	phosphate	ADJ
fnp-5497	31	13	buffered	buffer	VERB
fnp-5497	31	14	saline	saline	NOUN
fnp-5497	31	15	(	(	PUNCT
fnp-5497	31	16	pbs	pbs	PROPN
fnp-5497	31	17	)	)	PUNCT
fnp-5497	31	18	,	,	PUNCT
fnp-5497	31	19	1	1	NUM
fnp-5497	31	20	 	 	SPACE
fnp-5497	31	21	%	%	NOUN
fnp-5497	31	22	(	(	PUNCT
fnp-5497	31	23	w	w	NOUN
fnp-5497	31	24	/	/	SYM
fnp-5497	31	25	v	v	NOUN
fnp-5497	31	26	)	)	PUNCT
fnp-5497	31	27	sarkosyl	sarkosyl	NOUN
fnp-5497	31	28	,	,	PUNCT
fnp-5497	31	29	and	and	CCONJ
fnp-5497	31	30	assembly	assembly	NOUN
fnp-5497	31	31	/	/	SYM
fnp-5497	31	32	hepes	hepe	NOUN
fnp-5497	31	33	(	(	PUNCT
fnp-5497	31	34	4-(2	4-(2	PROPN
fnp-5497	31	35	-	-	PUNCT
fnp-5497	31	36	hydroxyethyl)-1	hydroxyethyl)-1	PROPN
fnp-5497	31	37	-	-	PUNCT
fnp-5497	31	38	piperazine	piperazine	NOUN
fnp-5497	31	39	ethanesulfonic	ethanesulfonic	ADJ
fnp-5497	31	40	acid	acid	NOUN
fnp-5497	31	41	)	)	PUNCT
fnp-5497	31	42	buffer	buffer	NOUN
fnp-5497	31	43	,	,	PUNCT
fnp-5497	31	44	all	all	PRON
fnp-5497	31	45	of	of	ADP
fnp-5497	31	46	which	which	PRON
fnp-5497	31	47	have	have	AUX
fnp-5497	31	48	been	be	AUX
fnp-5497	31	49	previously	previously	ADV
fnp-5497	31	50	documented	document	VERB
fnp-5497	31	51	for	for	ADP
fnp-5497	31	52	suspending	suspend	VERB
fnp-5497	31	53	whole	whole	ADJ
fnp-5497	31	54	tau	tau	PROPN
fnp-5497	31	55	fibrils6,8,9	fibrils6,8,9	NOUN
fnp-5497	31	56	.	.	PUNCT
fnp-5497	32	1	tau	tau	PROPN
fnp-5497	32	2	dgae	dgae	NOUN
fnp-5497	32	3	fibrils	fibril	NOUN
fnp-5497	32	4	maintained	maintain	VERB
fnp-5497	32	5	their	their	PRON
fnp-5497	32	6	aggregated	aggregate	VERB
fnp-5497	32	7	form	form	NOUN
fnp-5497	32	8	more	more	ADV
fnp-5497	32	9	effectively	effectively	ADV
fnp-5497	32	10	when	when	SCONJ
fnp-5497	32	11	diluted	dilute	VERB
fnp-5497	32	12	in	in	ADP
fnp-5497	32	13	1	1	NUM
fnp-5497	32	14	 	 	SPACE
fnp-5497	32	15	%	%	NOUN
fnp-5497	32	16	sarkosyl	sarkosyl	NOUN
fnp-5497	32	17	(	(	PUNCT
fnp-5497	32	18	figure	figure	VERB
fnp-5497	32	19	 	 	SPACE
fnp-5497	32	20	1a	1a	NOUN
fnp-5497	32	21	)	)	PUNCT
fnp-5497	32	22	.	.	PUNCT
fnp-5497	33	1	no	no	DET
fnp-5497	33	2	significant	significant	ADJ
fnp-5497	33	3	difference	difference	NOUN
fnp-5497	33	4	in	in	ADP
fnp-5497	33	5	sensitivity	sensitivity	NOUN
fnp-5497	33	6	was	be	AUX
fnp-5497	33	7	observed	observe	VERB
fnp-5497	33	8	between	between	ADP
fnp-5497	33	9	nitrocellulose	nitrocellulose	NOUN
fnp-5497	33	10	membranes	membrane	NOUN
fnp-5497	33	11	with	with	ADP
fnp-5497	33	12	pore	pore	ADJ
fnp-5497	33	13	sizes	size	NOUN
fnp-5497	33	14	of	of	ADP
fnp-5497	33	15	0.2	0.2	NUM
fnp-5497	33	16	 	 	SPACE
fnp-5497	33	17	μm	μm	NUM
fnp-5497	33	18	and	and	CCONJ
fnp-5497	33	19	0.45	0.45	NUM
fnp-5497	33	20	 	 	SPACE
fnp-5497	33	21	μm	μm	NOUN
fnp-5497	33	22	(	(	PUNCT
fnp-5497	33	23	figure	figure	NOUN
fnp-5497	33	24	 	 	SPACE
fnp-5497	33	25	1a	1a	NOUN
fnp-5497	33	26	)	)	PUNCT
fnp-5497	33	27	.	.	PUNCT
fnp-5497	34	1	as	as	ADV
fnp-5497	34	2	little	little	ADJ
fnp-5497	34	3	as	as	ADP
fnp-5497	34	4	7.8	7.8	NUM
fnp-5497	34	5	 	 	SPACE
fnp-5497	34	6	ng	ng	PROPN
fnp-5497	34	7	of	of	ADP
fnp-5497	34	8	dgae	dgae	NOUN
fnp-5497	34	9	fibrils	fibril	NOUN
fnp-5497	34	10	could	could	AUX
fnp-5497	34	11	be	be	AUX
fnp-5497	34	12	detected	detect	VERB
fnp-5497	34	13	using	use	VERB
fnp-5497	34	14	a	a	DET
fnp-5497	34	15	0.45	0.45	NUM
fnp-5497	34	16	 	 	SPACE
fnp-5497	34	17	μm	μm	NUM
fnp-5497	34	18	pore	pore	ADJ
fnp-5497	34	19	size	size	NOUN
fnp-5497	34	20	nitrocellulose	nitrocellulose	NOUN
fnp-5497	34	21	membrane	membrane	NOUN
fnp-5497	34	22	when	when	SCONJ
fnp-5497	34	23	diluted	dilute	VERB
fnp-5497	34	24	in	in	ADP
fnp-5497	34	25	1	1	NUM
fnp-5497	34	26	 	 	SPACE
fnp-5497	34	27	%	%	NOUN
fnp-5497	34	28	sarkosyl	sarkosyl	NOUN
fnp-5497	34	29	(	(	PUNCT
fnp-5497	34	30	figures	figure	NOUN
fnp-5497	34	31	 	 	SPACE
fnp-5497	34	32	1b	1b	NUM
fnp-5497	34	33	and	and	CCONJ
fnp-5497	34	34	1c	1c	NOUN
fnp-5497	34	35	)	)	PUNCT
fnp-5497	34	36	.	.	PUNCT
fnp-5497	35	1	figure	figure	NOUN
fnp-5497	35	2	1	1	NUM
fnp-5497	35	3	.	.	PUNCT
fnp-5497	35	4	optimal	optimal	ADJ
fnp-5497	35	5	sensitivity	sensitivity	NOUN
fnp-5497	35	6	in	in	ADP
fnp-5497	35	7	detecting	detect	VERB
fnp-5497	35	8	dgae	dgae	NOUN
fnp-5497	35	9	tau	tau	PROPN
fnp-5497	35	10	fibrils	fibril	NOUN
fnp-5497	35	11	is	be	AUX
fnp-5497	35	12	achieved	achieve	VERB
fnp-5497	35	13	using	use	VERB
fnp-5497	35	14	a	a	DET
fnp-5497	35	15	1	1	NUM
fnp-5497	35	16	 	 	SPACE
fnp-5497	35	17	%	%	NOUN
fnp-5497	35	18	sarkosyl	sarkosyl	NOUN
fnp-5497	35	19	solution	solution	NOUN
fnp-5497	35	20	in	in	ADP
fnp-5497	35	21	conjunction	conjunction	NOUN
fnp-5497	35	22	with	with	ADP
fnp-5497	35	23	a	a	DET
fnp-5497	35	24	nitrocellulose	nitrocellulose	NOUN
fnp-5497	35	25	membrane	membrane	NOUN
fnp-5497	35	26	a.	a.	NOUN
fnp-5497	35	27	dgae	dgae	PROPN
fnp-5497	35	28	tau	tau	PROPN
fnp-5497	35	29	protein	protein	NOUN
fnp-5497	35	30	(	(	PUNCT
fnp-5497	35	31	100	100	NUM
fnp-5497	35	32	 	 	SPACE
fnp-5497	35	33	ng	ng	PROPN
fnp-5497	35	34	)	)	PUNCT
fnp-5497	35	35	was	be	AUX
fnp-5497	35	36	applied	apply	VERB
fnp-5497	35	37	in	in	ADP
fnp-5497	35	38	duplicate	duplicate	NOUN
fnp-5497	35	39	per	per	ADP
fnp-5497	35	40	well	well	ADV
fnp-5497	35	41	(	(	PUNCT
fnp-5497	35	42	50	50	NUM
fnp-5497	35	43	 	 	SPACE
fnp-5497	35	44	μl	μl	ADJ
fnp-5497	35	45	/	/	SYM
fnp-5497	35	46	well	well	NOUN
fnp-5497	35	47	)	)	PUNCT
fnp-5497	35	48	to	to	ADP
fnp-5497	35	49	nitrocellulose	nitrocellulose	NOUN
fnp-5497	35	50	membranes	membrane	NOUN
fnp-5497	35	51	and	and	CCONJ
fnp-5497	35	52	pvdf	pvdf	PROPN
fnp-5497	35	53	membranes	membrane	NOUN
fnp-5497	35	54	pre	pre	VERB
fnp-5497	35	55	-	-	VERB
fnp-5497	35	56	treated	treat	VERB
fnp-5497	35	57	with	with	ADP
fnp-5497	35	58	methanol	methanol	NOUN
fnp-5497	35	59	for	for	ADP
fnp-5497	35	60	30	30	NUM
fnp-5497	35	61	seconds	second	NOUN
fnp-5497	35	62	using	use	VERB
fnp-5497	35	63	a	a	DET
fnp-5497	35	64	96	96	NUM
fnp-5497	35	65	-	-	PUNCT
fnp-5497	35	66	well	well	NOUN
fnp-5497	35	67	dot	dot	NOUN
fnp-5497	35	68	-	-	PUNCT
fnp-5497	35	69	blot	blot	NOUN
fnp-5497	35	70	apparatus	apparatus	NOUN
fnp-5497	35	71	and	and	CCONJ
fnp-5497	35	72	visualized	visualize	VERB
fnp-5497	35	73	with	with	ADP
fnp-5497	35	74	an	an	DET
fnp-5497	35	75	anti	anti	ADJ
fnp-5497	35	76	-	-	ADJ
fnp-5497	35	77	dgae	dgae	ADJ
fnp-5497	35	78	antibody	antibody	NOUN
fnp-5497	35	79	.	.	PUNCT
fnp-5497	36	1	similar	similar	ADJ
fnp-5497	36	2	results	result	NOUN
fnp-5497	36	3	were	be	AUX
fnp-5497	36	4	obtained	obtain	VERB
fnp-5497	36	5	from	from	ADP
fnp-5497	36	6	at	at	ADV
fnp-5497	36	7	least	least	ADV
fnp-5497	36	8	three	three	NUM
fnp-5497	36	9	independent	independent	ADJ
fnp-5497	36	10	experiments	experiment	NOUN
fnp-5497	36	11	.	.	PUNCT
fnp-5497	37	1	b.	b.	PROPN
fnp-5497	38	1	different	different	ADJ
fnp-5497	38	2	amounts	amount	NOUN
fnp-5497	38	3	of	of	ADP
fnp-5497	38	4	dgae	dgae	NOUN
fnp-5497	38	5	tau	tau	PROPN
fnp-5497	38	6	protein	protein	NOUN
fnp-5497	38	7	were	be	AUX
fnp-5497	38	8	processed	process	VERB
fnp-5497	38	9	similarly	similarly	ADV
fnp-5497	38	10	,	,	PUNCT
fnp-5497	38	11	representative	representative	NOUN
fnp-5497	38	12	of	of	ADP
fnp-5497	38	13	three	three	NUM
fnp-5497	38	14	independent	independent	ADJ
fnp-5497	38	15	experiments	experiment	NOUN
fnp-5497	38	16	.	.	PUNCT
fnp-5497	39	1	c.	c.	PROPN
fnp-5497	39	2	densitometry	densitometry	PROPN
fnp-5497	39	3	analysis	analysis	NOUN
fnp-5497	39	4	was	be	AUX
fnp-5497	39	5	performed	perform	VERB
fnp-5497	39	6	using	use	VERB
fnp-5497	39	7	bio	bio	PROPN
fnp-5497	39	8	-	-	PROPN
fnp-5497	39	9	rad	rad	ADJ
fnp-5497	39	10	 	 	SPACE
fnp-5497	39	11	imagelab	imagelab	NOUN
fnp-5497	39	12	 	 	SPACE
fnp-5497	39	13	6.1	6.1	NUM
fnp-5497	39	14	,	,	PUNCT
fnp-5497	39	15	with	with	ADP
fnp-5497	39	16	results	result	NOUN
fnp-5497	39	17	normalized	normalize	VERB
fnp-5497	39	18	to	to	ADP
fnp-5497	39	19	the	the	DET
fnp-5497	39	20	highest	high	ADJ
fnp-5497	39	21	tau	tau	PROPN
fnp-5497	39	22	fibril	fibril	ADJ
fnp-5497	39	23	concentration	concentration	NOUN
fnp-5497	39	24	(	(	PUNCT
fnp-5497	39	25	1000	1000	NUM
fnp-5497	39	26	 	 	SPACE
fnp-5497	39	27	ng	ng	PROPN
fnp-5497	39	28	)	)	PUNCT
fnp-5497	39	29	in	in	ADP
fnp-5497	39	30	1	1	NUM
fnp-5497	39	31	 	 	SPACE
fnp-5497	39	32	%	%	NOUN
fnp-5497	39	33	sarkosyl	sarkosyl	NOUN
fnp-5497	39	34	from	from	ADP
fnp-5497	39	35	three	three	NUM
fnp-5497	39	36	independent	independent	ADJ
fnp-5497	39	37	experiments	experiment	NOUN
fnp-5497	39	38	.	.	PUNCT
fnp-5497	40	1	using	use	VERB
fnp-5497	40	2	sds	sds	NOUN
fnp-5497	40	3	-	-	PUNCT
fnp-5497	40	4	page	page	NOUN
fnp-5497	40	5	we	we	PRON
fnp-5497	40	6	found	find	VERB
fnp-5497	40	7	that	that	SCONJ
fnp-5497	40	8	the	the	DET
fnp-5497	40	9	majority	majority	NOUN
fnp-5497	40	10	of	of	ADP
fnp-5497	40	11	dgae	dgae	NOUN
fnp-5497	40	12	tau	tau	PROPN
fnp-5497	40	13	aggregates	aggregate	NOUN
fnp-5497	40	14	were	be	AUX
fnp-5497	40	15	reduced	reduce	VERB
fnp-5497	40	16	to	to	ADP
fnp-5497	40	17	monomers	monomer	NOUN
fnp-5497	40	18	when	when	SCONJ
fnp-5497	40	19	treated	treat	VERB
fnp-5497	40	20	with	with	ADP
fnp-5497	40	21	laemmli	laemmli	NOUN
fnp-5497	40	22	sample	sample	NOUN
fnp-5497	40	23	buffers	buffer	NOUN
fnp-5497	40	24	containing	contain	VERB
fnp-5497	40	25	sds	sds	PROPN
fnp-5497	40	26	or	or	CCONJ
fnp-5497	40	27	lithium	lithium	NOUN
fnp-5497	40	28	dodecyl	dodecyl	PROPN
fnp-5497	40	29	phosphate	phosphate	NOUN
fnp-5497	40	30	(	(	PUNCT
fnp-5497	40	31	lds	lds	PROPN
fnp-5497	40	32	)	)	PUNCT
fnp-5497	40	33	,	,	PUNCT
fnp-5497	40	34	regardless	regardless	ADV
fnp-5497	40	35	of	of	ADP
fnp-5497	40	36	heat	heat	NOUN
fnp-5497	40	37	denaturation	denaturation	NOUN
fnp-5497	40	38	and	and	CCONJ
fnp-5497	40	39	reducing	reduce	VERB
fnp-5497	40	40	processes	process	NOUN
fnp-5497	40	41	(	(	PUNCT
fnp-5497	40	42	figure	figure	NOUN
fnp-5497	40	43	 	 	SPACE
fnp-5497	40	44	2a	2a	NUM
fnp-5497	40	45	)	)	PUNCT
fnp-5497	40	46	.	.	PUNCT
fnp-5497	41	1	to	to	PART
fnp-5497	41	2	corroborate	corroborate	VERB
fnp-5497	41	3	our	our	PRON
fnp-5497	41	4	observations	observation	NOUN
fnp-5497	41	5	,	,	PUNCT
fnp-5497	41	6	we	we	PRON
fnp-5497	41	7	subjected	subject	VERB
fnp-5497	41	8	protein	protein	NOUN
fnp-5497	41	9	samples	sample	NOUN
fnp-5497	41	10	,	,	PUNCT
fnp-5497	41	11	prepared	prepare	VERB
fnp-5497	41	12	in	in	ADP
fnp-5497	41	13	pbs	pbs	PROPN
fnp-5497	41	14	,	,	PUNCT
fnp-5497	41	15	sds	sds	PROPN
fnp-5497	41	16	,	,	PUNCT
fnp-5497	41	17	and	and	CCONJ
fnp-5497	41	18	sarkosyl	sarkosyl	NOUN
fnp-5497	41	19	,	,	PUNCT
fnp-5497	41	20	to	to	ADP
fnp-5497	41	21	native	native	ADJ
fnp-5497	41	22	-	-	PUNCT
fnp-5497	41	23	gel	gel	NOUN
fnp-5497	41	24	page	page	NOUN
fnp-5497	41	25	followed	follow	VERB
fnp-5497	41	26	by	by	ADP
fnp-5497	41	27	western	western	ADJ
fnp-5497	41	28	blotting	blotting	NOUN
fnp-5497	41	29	.	.	PUNCT
fnp-5497	42	1	both	both	CCONJ
fnp-5497	42	2	the	the	DET
fnp-5497	42	3	monomeric	monomeric	ADJ
fnp-5497	42	4	and	and	CCONJ
fnp-5497	42	5	fibrillar	fibrillar	ADJ
fnp-5497	42	6	forms	form	NOUN
fnp-5497	42	7	of	of	ADP
fnp-5497	42	8	tau	tau	PROPN
fnp-5497	42	9	dgae	dgae	PROPN
fnp-5497	42	10	protein	protein	NOUN
fnp-5497	42	11	,	,	PUNCT
fnp-5497	42	12	when	when	SCONJ
fnp-5497	42	13	in	in	ADP
fnp-5497	42	14	pbs	pbs	PROPN
fnp-5497	42	15	,	,	PUNCT
fnp-5497	42	16	remained	remain	VERB
fnp-5497	42	17	in	in	ADP
fnp-5497	42	18	the	the	DET
fnp-5497	42	19	wells	well	NOUN
fnp-5497	42	20	,	,	PUNCT
fnp-5497	42	21	presumably	presumably	ADV
fnp-5497	42	22	due	due	ADP
fnp-5497	42	23	to	to	ADP
fnp-5497	42	24	the	the	DET
fnp-5497	42	25	high	high	ADJ
fnp-5497	42	26	basic	basic	ADJ
fnp-5497	42	27	nature	nature	NOUN
fnp-5497	42	28	of	of	ADP
fnp-5497	42	29	tau	tau	PROPN
fnp-5497	42	30	dgae	dgae	PROPN
fnp-5497	42	31	,	,	PUNCT
fnp-5497	42	32	which	which	PRON
fnp-5497	42	33	has	have	VERB
fnp-5497	42	34	a	a	DET
fnp-5497	42	35	theoretical	theoretical	ADJ
fnp-5497	42	36	isoelectric	isoelectric	ADJ
fnp-5497	42	37	point	point	NOUN
fnp-5497	42	38	of	of	ADP
fnp-5497	42	39	9.59	9.59	NUM
fnp-5497	42	40	and	and	CCONJ
fnp-5497	42	41	thus	thus	ADV
fnp-5497	42	42	would	would	AUX
fnp-5497	42	43	be	be	AUX
fnp-5497	42	44	positively	positively	ADV
fnp-5497	42	45	charged	charge	VERB
fnp-5497	42	46	in	in	ADP
fnp-5497	42	47	the	the	DET
fnp-5497	42	48	absence	absence	NOUN
fnp-5497	42	49	of	of	ADP
fnp-5497	42	50	an	an	DET
fnp-5497	42	51	anionic	anionic	ADJ
fnp-5497	42	52	surfactant10	surfactant10	NOUN
fnp-5497	42	53	.	.	PUNCT
fnp-5497	43	1	when	when	SCONJ
fnp-5497	43	2	subjected	subject	VERB
fnp-5497	43	3	to	to	ADP
fnp-5497	43	4	anionic	anionic	ADJ
fnp-5497	43	5	detergents	detergent	NOUN
fnp-5497	43	6	,	,	PUNCT
fnp-5497	43	7	the	the	DET
fnp-5497	43	8	dgae	dgae	NOUN
fnp-5497	43	9	tau	tau	PROPN
fnp-5497	43	10	proteins	protein	NOUN
fnp-5497	43	11	displayed	display	VERB
fnp-5497	43	12	distinct	distinct	ADJ
fnp-5497	43	13	behaviors	behavior	NOUN
fnp-5497	43	14	:	:	PUNCT
fnp-5497	43	15	in	in	ADP
fnp-5497	43	16	sds	sds	PROPN
fnp-5497	43	17	,	,	PUNCT
fnp-5497	43	18	the	the	DET
fnp-5497	43	19	fibrils	fibril	NOUN
fnp-5497	43	20	predominantly	predominantly	ADV
fnp-5497	43	21	were	be	AUX
fnp-5497	43	22	broken	break	VERB
fnp-5497	43	23	down	down	ADP
fnp-5497	43	24	into	into	ADP
fnp-5497	43	25	monomers	monomer	NOUN
fnp-5497	43	26	,	,	PUNCT
fnp-5497	43	27	whereas	whereas	SCONJ
fnp-5497	43	28	in	in	ADP
fnp-5497	43	29	sarkosyl	sarkosyl	NOUN
fnp-5497	43	30	,	,	PUNCT
fnp-5497	43	31	they	they	PRON
fnp-5497	43	32	retained	retain	VERB
fnp-5497	43	33	their	their	PRON
fnp-5497	43	34	fibrillar	fibrillar	ADJ
fnp-5497	43	35	integrity	integrity	NOUN
fnp-5497	43	36	(	(	PUNCT
fnp-5497	43	37	figure	figure	NOUN
fnp-5497	43	38	 	 	SPACE
fnp-5497	43	39	2b	2b	NOUN
fnp-5497	43	40	)	)	PUNCT
fnp-5497	43	41	.	.	PUNCT
fnp-5497	44	1	figure	figure	NOUN
fnp-5497	44	2	2	2	NUM
fnp-5497	44	3	.	.	PUNCT
fnp-5497	45	1	the	the	DET
fnp-5497	45	2	majority	majority	NOUN
fnp-5497	45	3	of	of	ADP
fnp-5497	45	4	dgae	dgae	NOUN
fnp-5497	45	5	tau	tau	PROPN
fnp-5497	45	6	aggregates	aggregate	NOUN
fnp-5497	45	7	are	be	AUX
fnp-5497	45	8	reduced	reduce	VERB
fnp-5497	45	9	to	to	ADP
fnp-5497	45	10	monomers	monomer	NOUN
fnp-5497	45	11	when	when	SCONJ
fnp-5497	45	12	treated	treat	VERB
fnp-5497	45	13	with	with	ADP
fnp-5497	45	14	sds	sds	PROPN
fnp-5497	45	15	a.	a.	NOUN
fnp-5497	45	16	dgae	dgae	PROPN
fnp-5497	45	17	tau	tau	PROPN
fnp-5497	45	18	protein	protein	NOUN
fnp-5497	45	19	(	(	PUNCT
fnp-5497	45	20	100	100	NUM
fnp-5497	45	21	 	 	SPACE
fnp-5497	45	22	ng	ng	PROPN
fnp-5497	45	23	/	/	SYM
fnp-5497	45	24	lane	lane	NOUN
fnp-5497	45	25	)	)	PUNCT
fnp-5497	45	26	was	be	AUX
fnp-5497	45	27	treated	treat	VERB
fnp-5497	45	28	with	with	ADP
fnp-5497	45	29	laemmli	laemmli	NOUN
fnp-5497	45	30	buffer	buffer	NOUN
fnp-5497	45	31	containing	contain	VERB
fnp-5497	45	32	sds	sds	NOUN
fnp-5497	45	33	or	or	CCONJ
fnp-5497	45	34	lithium	lithium	NOUN
fnp-5497	45	35	dodecyl	dodecyl	PROPN
fnp-5497	45	36	sulfate	sulfate	NOUN
fnp-5497	45	37	(	(	PUNCT
fnp-5497	45	38	lds	lds	PROPN
fnp-5497	45	39	)	)	PUNCT
fnp-5497	45	40	,	,	PUNCT
fnp-5497	45	41	with	with	ADP
fnp-5497	45	42	and	and	CCONJ
fnp-5497	45	43	without	without	ADP
fnp-5497	45	44	100	100	NUM
fnp-5497	45	45	 	 	SPACE
fnp-5497	45	46	mm	mm	PROPN
fnp-5497	45	47	dithiothreitol	dithiothreitol	NOUN
fnp-5497	45	48	(	(	PUNCT
fnp-5497	45	49	dtt	dtt	NOUN
fnp-5497	45	50	)	)	PUNCT
fnp-5497	45	51	,	,	PUNCT
fnp-5497	45	52	and	and	CCONJ
fnp-5497	45	53	processed	process	VERB
fnp-5497	45	54	both	both	CCONJ
fnp-5497	45	55	unboiled	unboiled	ADJ
fnp-5497	45	56	and	and	CCONJ
fnp-5497	45	57	boiled	boil	VERB
fnp-5497	45	58	for	for	ADP
fnp-5497	45	59	10	10	NUM
fnp-5497	45	60	 	 	SPACE
fnp-5497	45	61	minutes	minute	NOUN
fnp-5497	45	62	.	.	PUNCT
fnp-5497	46	1	proteins	protein	NOUN
fnp-5497	46	2	were	be	AUX
fnp-5497	46	3	then	then	ADV
fnp-5497	46	4	subjected	subject	VERB
fnp-5497	46	5	to	to	ADP
fnp-5497	46	6	sds	sds	NOUN
fnp-5497	46	7	-	-	PUNCT
fnp-5497	46	8	page	page	NOUN
fnp-5497	46	9	and	and	CCONJ
fnp-5497	46	10	visualized	visualize	VERB
fnp-5497	46	11	using	use	VERB
fnp-5497	46	12	coomassie	coomassie	ADJ
fnp-5497	46	13	brilliant	brilliant	ADJ
fnp-5497	46	14	blue	blue	ADJ
fnp-5497	46	15	staining	staining	NOUN
fnp-5497	46	16	.	.	PUNCT
fnp-5497	47	1	b.	b.	NOUN
fnp-5497	47	2	samples	sample	NOUN
fnp-5497	47	3	in	in	ADP
fnp-5497	47	4	pbs	pbs	PROPN
fnp-5497	47	5	,	,	PUNCT
fnp-5497	47	6	2	2	NUM
fnp-5497	47	7	 	 	SPACE
fnp-5497	47	8	%	%	NOUN
fnp-5497	47	9	sds	sds	NOUN
fnp-5497	47	10	or	or	CCONJ
fnp-5497	47	11	1	1	NUM
fnp-5497	47	12	 	 	SPACE
fnp-5497	47	13	%	%	NOUN
fnp-5497	47	14	sarkosyl	sarkosyl	NOUN
fnp-5497	47	15	(	(	PUNCT
fnp-5497	47	16	100	100	NUM
fnp-5497	47	17	 	 	SPACE
fnp-5497	47	18	ng	ng	PROPN
fnp-5497	47	19	/	/	SYM
fnp-5497	47	20	lane	lane	NOUN
fnp-5497	47	21	)	)	PUNCT
fnp-5497	47	22	were	be	AUX
fnp-5497	47	23	analyzed	analyze	VERB
fnp-5497	47	24	using	use	VERB
fnp-5497	47	25	native	native	ADJ
fnp-5497	47	26	page	page	NOUN
fnp-5497	47	27	and	and	CCONJ
fnp-5497	47	28	western	western	ADJ
fnp-5497	47	29	blotting	blotting	NOUN
fnp-5497	47	30	,	,	PUNCT
fnp-5497	47	31	detecting	detect	VERB
fnp-5497	47	32	tau	tau	PROPN
fnp-5497	47	33	protein	protein	NOUN
fnp-5497	47	34	with	with	ADP
fnp-5497	47	35	an	an	DET
fnp-5497	47	36	anti	anti	ADJ
fnp-5497	47	37	-	-	ADJ
fnp-5497	47	38	dgae	dgae	ADJ
fnp-5497	47	39	tau	tau	NOUN
fnp-5497	47	40	antibody	antibody	NOUN
fnp-5497	47	41	.	.	PUNCT
fnp-5497	48	1	data	datum	NOUN
fnp-5497	48	2	shown	show	VERB
fnp-5497	48	3	are	be	AUX
fnp-5497	48	4	representative	representative	NOUN
fnp-5497	48	5	of	of	ADP
fnp-5497	48	6	two	two	NUM
fnp-5497	48	7	independent	independent	ADJ
fnp-5497	48	8	experiments	experiment	NOUN
fnp-5497	48	9	.	.	PUNCT
fnp-5497	49	1	taken	take	VERB
fnp-5497	49	2	together	together	ADV
fnp-5497	49	3	,	,	PUNCT
fnp-5497	49	4	these	these	DET
fnp-5497	49	5	data	datum	NOUN
fnp-5497	49	6	imply	imply	VERB
fnp-5497	49	7	that	that	SCONJ
fnp-5497	49	8	for	for	ADP
fnp-5497	49	9	effective	effective	ADJ
fnp-5497	49	10	fta	fta	PROPN
fnp-5497	49	11	detection	detection	NOUN
fnp-5497	49	12	,	,	PUNCT
fnp-5497	49	13	tau	tau	PROPN
fnp-5497	49	14	dgae	dgae	NOUN
fnp-5497	49	15	samples	sample	NOUN
fnp-5497	49	16	should	should	AUX
fnp-5497	49	17	be	be	AUX
fnp-5497	49	18	diluted	dilute	VERB
fnp-5497	49	19	in	in	ADP
fnp-5497	49	20	sarkosyl	sarkosyl	NOUN
fnp-5497	49	21	that	that	PRON
fnp-5497	49	22	preserves	preserve	VERB
fnp-5497	49	23	their	their	PRON
fnp-5497	49	24	aggregated	aggregated	ADJ
fnp-5497	49	25	state	state	NOUN
fnp-5497	49	26	.	.	PUNCT
fnp-5497	50	1	discussion	discussion	NOUN
fnp-5497	50	2	the	the	DET
fnp-5497	50	3	introduction	introduction	NOUN
fnp-5497	50	4	of	of	ADP
fnp-5497	50	5	truncated	truncated	ADJ
fnp-5497	50	6	tau	tau	PROPN
fnp-5497	50	7	variants	variant	NOUN
fnp-5497	50	8	such	such	ADJ
fnp-5497	50	9	as	as	ADP
fnp-5497	50	10	dgae	dgae	PROPN
fnp-5497	50	11	tau	tau	PROPN
fnp-5497	50	12	offers	offer	VERB
fnp-5497	50	13	a	a	DET
fnp-5497	50	14	more	more	ADV
fnp-5497	50	15	representative	representative	NOUN
fnp-5497	50	16	in	in	ADP
fnp-5497	50	17	vitro	vitro	X
fnp-5497	50	18	model	model	NOUN
fnp-5497	50	19	for	for	ADP
fnp-5497	50	20	studying	study	VERB
fnp-5497	50	21	tau	tau	PROPN
fnp-5497	50	22	aggregation	aggregation	NOUN
fnp-5497	50	23	as	as	SCONJ
fnp-5497	50	24	compared	compare	VERB
fnp-5497	50	25	polyanion	polyanion	PROPN
fnp-5497	50	26	tau	tau	PROPN
fnp-5497	50	27	aggregation	aggregation	NOUN
fnp-5497	50	28	.	.	PUNCT
fnp-5497	51	1	we	we	PRON
fnp-5497	51	2	optimized	optimize	VERB
fnp-5497	51	3	a	a	DET
fnp-5497	51	4	protocol	protocol	NOUN
fnp-5497	51	5	for	for	ADP
fnp-5497	51	6	the	the	DET
fnp-5497	51	7	fta	fta	PROPN
fnp-5497	51	8	using	use	VERB
fnp-5497	51	9	dgae	dgae	NOUN
fnp-5497	51	10	tau	tau	PROPN
fnp-5497	51	11	aggregates	aggregate	NOUN
fnp-5497	51	12	,	,	PUNCT
fnp-5497	51	13	representing	represent	VERB
fnp-5497	51	14	a	a	DET
fnp-5497	51	15	notable	notable	ADJ
fnp-5497	51	16	stride	stride	NOUN
fnp-5497	51	17	towards	towards	ADP
fnp-5497	51	18	more	more	ADV
fnp-5497	51	19	accurate	accurate	ADJ
fnp-5497	51	20	and	and	CCONJ
fnp-5497	51	21	physiologically	physiologically	ADV
fnp-5497	51	22	relevant	relevant	ADJ
fnp-5497	51	23	in	in	ADP
fnp-5497	51	24	vitro	vitro	X
fnp-5497	51	25	models	model	NOUN
fnp-5497	51	26	.	.	PUNCT
fnp-5497	52	1	the	the	DET
fnp-5497	52	2	use	use	NOUN
fnp-5497	52	3	of	of	ADP
fnp-5497	52	4	nitrocellulose	nitrocellulose	NOUN
fnp-5497	52	5	membranes	membrane	NOUN
fnp-5497	52	6	in	in	ADP
fnp-5497	52	7	the	the	DET
fnp-5497	52	8	fta	fta	PROPN
fnp-5497	52	9	emerged	emerge	VERB
fnp-5497	52	10	as	as	ADP
fnp-5497	52	11	a	a	DET
fnp-5497	52	12	key	key	ADJ
fnp-5497	52	13	factor	factor	NOUN
fnp-5497	52	14	in	in	ADP
fnp-5497	52	15	achieving	achieve	VERB
fnp-5497	52	16	high	high	ADJ
fnp-5497	52	17	sensitivity	sensitivity	NOUN
fnp-5497	52	18	in	in	ADP
fnp-5497	52	19	detecting	detect	VERB
fnp-5497	52	20	tau	tau	PROPN
fnp-5497	52	21	aggregates	aggregate	NOUN
fnp-5497	52	22	,	,	PUNCT
fnp-5497	52	23	thereby	thereby	ADV
fnp-5497	52	24	providing	provide	VERB
fnp-5497	52	25	a	a	DET
fnp-5497	52	26	robust	robust	ADJ
fnp-5497	52	27	alternative	alternative	NOUN
fnp-5497	52	28	to	to	ADP
fnp-5497	52	29	tht	tht	NOUN
fnp-5497	52	30	fluorescence	fluorescence	ADJ
fnp-5497	52	31	assays	assay	NOUN
fnp-5497	52	32	,	,	PUNCT
fnp-5497	52	33	which	which	PRON
fnp-5497	52	34	are	be	AUX
fnp-5497	52	35	prone	prone	ADJ
fnp-5497	52	36	to	to	AUX
fnp-5497	52	37	inconsistency	inconsistency	VERB
fnp-5497	52	38	.	.	PUNCT
fnp-5497	53	1	moreover	moreover	ADV
fnp-5497	53	2	,	,	PUNCT
fnp-5497	53	3	our	our	PRON
fnp-5497	53	4	exploration	exploration	NOUN
fnp-5497	53	5	of	of	ADP
fnp-5497	53	6	different	different	ADJ
fnp-5497	53	7	solvents	solvent	NOUN
fnp-5497	53	8	such	such	ADJ
fnp-5497	53	9	as	as	ADP
fnp-5497	53	10	pbs	pbs	PROPN
fnp-5497	53	11	,	,	PUNCT
fnp-5497	53	12	1	1	NUM
fnp-5497	53	13	 	 	SPACE
fnp-5497	53	14	%	%	NOUN
fnp-5497	53	15	sarkosyl	sarkosyl	NOUN
fnp-5497	53	16	,	,	PUNCT
fnp-5497	53	17	and	and	CCONJ
fnp-5497	53	18	assembly	assembly	NOUN
fnp-5497	53	19	/	/	SYM
fnp-5497	53	20	hepes	hepe	NOUN
fnp-5497	53	21	buffer	buffer	NOUN
fnp-5497	53	22	for	for	SCONJ
fnp-5497	53	23	fta	fta	PROPN
fnp-5497	53	24	further	far	ADV
fnp-5497	53	25	refines	refine	VERB
fnp-5497	53	26	the	the	DET
fnp-5497	53	27	approach	approach	NOUN
fnp-5497	53	28	to	to	PART
fnp-5497	53	29	study	study	VERB
fnp-5497	53	30	tau	tau	PROPN
fnp-5497	53	31	aggregation	aggregation	NOUN
fnp-5497	53	32	.	.	PUNCT
fnp-5497	54	1	although	although	SCONJ
fnp-5497	54	2	sarkosyl	sarkosyl	NOUN
fnp-5497	54	3	has	have	AUX
fnp-5497	54	4	traditionally	traditionally	ADV
fnp-5497	54	5	been	be	AUX
fnp-5497	54	6	used	use	VERB
fnp-5497	54	7	to	to	PART
fnp-5497	54	8	prepare	prepare	VERB
fnp-5497	54	9	lysates	lysate	NOUN
fnp-5497	54	10	from	from	ADP
fnp-5497	54	11	brain	brain	NOUN
fnp-5497	54	12	tissues	tissue	NOUN
fnp-5497	54	13	and	and	CCONJ
fnp-5497	54	14	from	from	ADP
fnp-5497	54	15	cultured	cultured	ADJ
fnp-5497	54	16	neurons	neuron	NOUN
fnp-5497	54	17	to	to	PART
fnp-5497	54	18	detect	detect	VERB
fnp-5497	54	19	tau	tau	PROPN
fnp-5497	54	20	aggregates	aggregate	NOUN
fnp-5497	54	21	,	,	PUNCT
fnp-5497	54	22	the	the	DET
fnp-5497	54	23	effectiveness	effectiveness	NOUN
fnp-5497	54	24	of	of	ADP
fnp-5497	54	25	sarkosyl	sarkosyl	NOUN
fnp-5497	54	26	in	in	ADP
fnp-5497	54	27	maintaining	maintain	VERB
fnp-5497	54	28	the	the	DET
fnp-5497	54	29	aggregated	aggregated	ADJ
fnp-5497	54	30	state	state	NOUN
fnp-5497	54	31	of	of	ADP
fnp-5497	54	32	tau	tau	PROPN
fnp-5497	54	33	dgae	dgae	NOUN
fnp-5497	54	34	fibrils	fibril	NOUN
fnp-5497	54	35	in	in	ADP
fnp-5497	54	36	vitro	vitro	X
fnp-5497	54	37	presents	present	VERB
fnp-5497	54	38	a	a	DET
fnp-5497	54	39	promising	promising	ADJ
fnp-5497	54	40	avenue	avenue	NOUN
fnp-5497	54	41	for	for	ADP
fnp-5497	54	42	future	future	ADJ
fnp-5497	54	43	studies	study	NOUN
fnp-5497	54	44	.	.	PUNCT
fnp-5497	55	1	the	the	DET
fnp-5497	55	2	underlying	underlying	ADJ
fnp-5497	55	3	mechanism	mechanism	NOUN
fnp-5497	55	4	for	for	ADP
fnp-5497	55	5	the	the	DET
fnp-5497	55	6	enhanced	enhanced	ADJ
fnp-5497	55	7	efficiency	efficiency	NOUN
fnp-5497	55	8	of	of	ADP
fnp-5497	55	9	sarkosyl	sarkosyl	NOUN
fnp-5497	55	10	in	in	ADP
fnp-5497	55	11	detecting	detect	VERB
fnp-5497	55	12	tau	tau	PROPN
fnp-5497	55	13	dgae	dgae	NOUN
fnp-5497	55	14	aggregates	aggregate	NOUN
fnp-5497	55	15	through	through	ADP
fnp-5497	55	16	fta	fta	PROPN
fnp-5497	55	17	compared	compare	VERB
fnp-5497	55	18	to	to	ADP
fnp-5497	55	19	pbs	pbs	PROPN
fnp-5497	55	20	and	and	CCONJ
fnp-5497	55	21	assembly	assembly	PROPN
fnp-5497	55	22	/	/	SYM
fnp-5497	55	23	hepes	hepe	NOUN
fnp-5497	55	24	buffer	buffer	NOUN
fnp-5497	55	25	remains	remain	VERB
fnp-5497	55	26	elusive	elusive	ADJ
fnp-5497	55	27	.	.	PUNCT
fnp-5497	56	1	we	we	PRON
fnp-5497	56	2	speculate	speculate	VERB
fnp-5497	56	3	that	that	SCONJ
fnp-5497	56	4	the	the	DET
fnp-5497	56	5	unique	unique	ADJ
fnp-5497	56	6	physicochemical	physicochemical	ADJ
fnp-5497	56	7	properties	property	NOUN
fnp-5497	56	8	of	of	ADP
fnp-5497	56	9	sarkosyl	sarkosyl	NOUN
fnp-5497	56	10	may	may	AUX
fnp-5497	56	11	interact	interact	VERB
fnp-5497	56	12	with	with	ADP
fnp-5497	56	13	tau	tau	PROPN
fnp-5497	56	14	aggregates	aggregate	NOUN
fnp-5497	56	15	in	in	ADP
fnp-5497	56	16	a	a	DET
fnp-5497	56	17	manner	manner	NOUN
fnp-5497	56	18	that	that	PRON
fnp-5497	56	19	is	be	AUX
fnp-5497	56	20	less	less	ADV
fnp-5497	56	21	denaturing	denaturing	NOUN
fnp-5497	56	22	than	than	ADP
fnp-5497	56	23	sds	sds	PROPN
fnp-5497	56	24	,	,	PUNCT
fnp-5497	56	25	or	or	CCONJ
fnp-5497	56	26	promotes	promote	VERB
fnp-5497	56	27	the	the	DET
fnp-5497	56	28	stabilization	stabilization	NOUN
fnp-5497	56	29	of	of	ADP
fnp-5497	56	30	tau	tau	PROPN
fnp-5497	56	31	aggregates	aggregate	NOUN
fnp-5497	56	32	,	,	PUNCT
fnp-5497	56	33	thus	thus	ADV
fnp-5497	56	34	facilitating	facilitate	VERB
fnp-5497	56	35	their	their	PRON
fnp-5497	56	36	capture	capture	NOUN
fnp-5497	56	37	on	on	ADP
fnp-5497	56	38	the	the	DET
fnp-5497	56	39	membrane	membrane	NOUN
fnp-5497	56	40	.	.	PUNCT
fnp-5497	57	1	further	further	ADJ
fnp-5497	57	2	investigations	investigation	NOUN
fnp-5497	57	3	are	be	AUX
fnp-5497	57	4	required	require	VERB
fnp-5497	57	5	to	to	PART
fnp-5497	57	6	delineate	delineate	VERB
fnp-5497	57	7	the	the	DET
fnp-5497	57	8	specific	specific	ADJ
fnp-5497	57	9	molecular	molecular	ADJ
fnp-5497	57	10	interactions	interaction	NOUN
fnp-5497	57	11	and	and	CCONJ
fnp-5497	57	12	biophysical	biophysical	ADJ
fnp-5497	57	13	changes	change	NOUN
fnp-5497	57	14	induced	induce	VERB
fnp-5497	57	15	by	by	ADP
fnp-5497	57	16	sarkosyl	sarkosyl	NOUN
fnp-5497	57	17	,	,	PUNCT
fnp-5497	57	18	which	which	PRON
fnp-5497	57	19	may	may	AUX
fnp-5497	57	20	offer	offer	VERB
fnp-5497	57	21	insights	insight	NOUN
fnp-5497	57	22	into	into	ADP
fnp-5497	57	23	its	its	PRON
fnp-5497	57	24	preferential	preferential	ADJ
fnp-5497	57	25	efficacy	efficacy	NOUN
fnp-5497	57	26	and	and	CCONJ
fnp-5497	57	27	may	may	AUX
fnp-5497	57	28	potentially	potentially	ADV
fnp-5497	57	29	uncover	uncover	VERB
fnp-5497	57	30	novel	novel	ADJ
fnp-5497	57	31	aspects	aspect	NOUN
fnp-5497	57	32	of	of	ADP
fnp-5497	57	33	tau	tau	PROPN
fnp-5497	57	34	aggregation	aggregation	NOUN
fnp-5497	57	35	dynamics	dynamic	NOUN
fnp-5497	57	36	.	.	PUNCT
fnp-5497	58	1	thus	thus	ADV
fnp-5497	58	2	,	,	PUNCT
fnp-5497	58	3	the	the	DET
fnp-5497	58	4	assay	assay	NOUN
fnp-5497	58	5	we	we	PRON
fnp-5497	58	6	developed	develop	VERB
fnp-5497	58	7	in	in	ADP
fnp-5497	58	8	this	this	DET
fnp-5497	58	9	study	study	NOUN
fnp-5497	58	10	is	be	AUX
fnp-5497	58	11	especially	especially	ADV
fnp-5497	58	12	relevant	relevant	ADJ
fnp-5497	58	13	given	give	VERB
fnp-5497	58	14	the	the	DET
fnp-5497	58	15	need	need	NOUN
fnp-5497	58	16	for	for	ADP
fnp-5497	58	17	more	more	ADV
fnp-5497	58	18	reliable	reliable	ADJ
fnp-5497	58	19	and	and	CCONJ
fnp-5497	58	20	reproducible	reproducible	ADJ
fnp-5497	58	21	methods	method	NOUN
fnp-5497	58	22	in	in	ADP
fnp-5497	58	23	tauopathy	tauopathy	ADJ
fnp-5497	58	24	research	research	NOUN
fnp-5497	58	25	,	,	PUNCT
fnp-5497	58	26	particularly	particularly	ADV
fnp-5497	58	27	in	in	ADP
fnp-5497	58	28	view	view	NOUN
fnp-5497	58	29	of	of	ADP
fnp-5497	58	30	its	its	PRON
fnp-5497	58	31	simple	simple	ADJ
fnp-5497	58	32	,	,	PUNCT
fnp-5497	58	33	efficient	efficient	ADJ
fnp-5497	58	34	,	,	PUNCT
fnp-5497	58	35	user	user	NOUN
fnp-5497	58	36	-	-	PUNCT
fnp-5497	58	37	friendly	friendly	ADJ
fnp-5497	58	38	and	and	CCONJ
fnp-5497	58	39	cost	cost	NOUN
fnp-5497	58	40	-	-	PUNCT
fnp-5497	58	41	effective	effective	ADJ
fnp-5497	58	42	approach	approach	NOUN
fnp-5497	58	43	.	.	PUNCT
fnp-5497	59	1	this	this	DET
fnp-5497	59	2	ease	ease	NOUN
fnp-5497	59	3	of	of	ADP
fnp-5497	59	4	use	use	NOUN
fnp-5497	59	5	and	and	CCONJ
fnp-5497	59	6	affordability	affordability	NOUN
fnp-5497	59	7	make	make	VERB
fnp-5497	59	8	fta	fta	PROPN
fnp-5497	59	9	an	an	DET
fnp-5497	59	10	accessible	accessible	ADJ
fnp-5497	59	11	tool	tool	NOUN
fnp-5497	59	12	for	for	ADP
fnp-5497	59	13	laboratories	laboratory	NOUN
fnp-5497	59	14	with	with	ADP
fnp-5497	59	15	varying	vary	VERB
fnp-5497	59	16	resources	resource	NOUN
fnp-5497	59	17	,	,	PUNCT
fnp-5497	59	18	democratizing	democratize	VERB
fnp-5497	59	19	research	research	NOUN
fnp-5497	59	20	in	in	ADP
fnp-5497	59	21	neurodegenerative	neurodegenerative	ADJ
fnp-5497	59	22	diseases	disease	NOUN
fnp-5497	59	23	.	.	PUNCT
fnp-5497	60	1	this	this	DET
fnp-5497	60	2	method	method	NOUN
fnp-5497	60	3	offers	offer	VERB
fnp-5497	60	4	a	a	DET
fnp-5497	60	5	practical	practical	ADJ
fnp-5497	60	6	alternative	alternative	NOUN
fnp-5497	60	7	to	to	ADP
fnp-5497	60	8	more	more	ADV
fnp-5497	60	9	complex	complex	ADJ
fnp-5497	60	10	and	and	CCONJ
fnp-5497	60	11	expensive	expensive	ADJ
fnp-5497	60	12	techniques	technique	NOUN
fnp-5497	60	13	,	,	PUNCT
fnp-5497	60	14	such	such	ADJ
fnp-5497	60	15	as	as	ADP
fnp-5497	60	16	em	em	PRON
fnp-5497	60	17	.	.	PUNCT
fnp-5497	61	1	while	while	SCONJ
fnp-5497	61	2	cryo	cryo	PROPN
fnp-5497	61	3	-	-	PUNCT
fnp-5497	61	4	em	em	PRON
fnp-5497	61	5	provides	provide	VERB
fnp-5497	61	6	unparalleled	unparalleled	ADJ
fnp-5497	61	7	detail	detail	NOUN
fnp-5497	61	8	in	in	ADP
fnp-5497	61	9	structural	structural	ADJ
fnp-5497	61	10	analysis	analysis	NOUN
fnp-5497	61	11	,	,	PUNCT
fnp-5497	61	12	its	its	PRON
fnp-5497	61	13	high	high	ADJ
fnp-5497	61	14	cost	cost	NOUN
fnp-5497	61	15	and	and	CCONJ
fnp-5497	61	16	technical	technical	ADJ
fnp-5497	61	17	requirements	requirement	NOUN
fnp-5497	61	18	can	can	AUX
fnp-5497	61	19	be	be	AUX
fnp-5497	61	20	prohibitive	prohibitive	ADJ
fnp-5497	61	21	.	.	PUNCT
fnp-5497	62	1	in	in	ADP
fnp-5497	62	2	contrast	contrast	NOUN
fnp-5497	62	3	,	,	PUNCT
fnp-5497	62	4	the	the	DET
fnp-5497	62	5	fta	fta	PROPN
fnp-5497	62	6	protocol	protocol	NOUN
fnp-5497	62	7	,	,	PUNCT
fnp-5497	62	8	especially	especially	ADV
fnp-5497	62	9	with	with	ADP
fnp-5497	62	10	the	the	DET
fnp-5497	62	11	use	use	NOUN
fnp-5497	62	12	of	of	ADP
fnp-5497	62	13	nitrocellulose	nitrocellulose	NOUN
fnp-5497	62	14	membranes	membrane	NOUN
fnp-5497	62	15	,	,	PUNCT
fnp-5497	62	16	presents	present	VERB
fnp-5497	62	17	a	a	DET
fnp-5497	62	18	straightforward	straightforward	ADJ
fnp-5497	62	19	and	and	CCONJ
fnp-5497	62	20	economical	economical	ADJ
fnp-5497	62	21	solution	solution	NOUN
fnp-5497	62	22	for	for	ADP
fnp-5497	62	23	initial	initial	ADJ
fnp-5497	62	24	screening	screening	NOUN
fnp-5497	62	25	and	and	CCONJ
fnp-5497	62	26	analysis	analysis	NOUN
fnp-5497	62	27	of	of	ADP
fnp-5497	62	28	authentic	authentic	ADJ
fnp-5497	62	29	tau	tau	PROPN
fnp-5497	62	30	aggregates	aggregate	NOUN
fnp-5497	62	31	.	.	PUNCT
fnp-5497	63	1	this	this	DET
fnp-5497	63	2	approach	approach	NOUN
fnp-5497	63	3	not	not	PART
fnp-5497	63	4	only	only	ADV
fnp-5497	63	5	facilitates	facilitate	VERB
fnp-5497	63	6	routine	routine	ADJ
fnp-5497	63	7	laboratory	laboratory	NOUN
fnp-5497	63	8	investigations	investigation	NOUN
fnp-5497	63	9	but	but	CCONJ
fnp-5497	63	10	may	may	AUX
fnp-5497	63	11	also	also	ADV
fnp-5497	63	12	serve	serve	VERB
fnp-5497	63	13	as	as	ADP
fnp-5497	63	14	a	a	DET
fnp-5497	63	15	critical	critical	ADJ
fnp-5497	63	16	preliminary	preliminary	ADJ
fnp-5497	63	17	screening	screening	NOUN
fnp-5497	63	18	step	step	NOUN
fnp-5497	63	19	before	before	ADP
fnp-5497	63	20	employing	employ	VERB
fnp-5497	63	21	more	more	ADV
fnp-5497	63	22	sophisticated	sophisticated	ADJ
fnp-5497	63	23	methods	method	NOUN
fnp-5497	63	24	.	.	PUNCT
fnp-5497	64	1	the	the	DET
fnp-5497	64	2	capacity	capacity	NOUN
fnp-5497	64	3	to	to	PART
fnp-5497	64	4	detect	detect	VERB
fnp-5497	64	5	minimal	minimal	ADJ
fnp-5497	64	6	quantities	quantity	NOUN
fnp-5497	64	7	of	of	ADP
fnp-5497	64	8	dgae	dgae	NOUN
fnp-5497	64	9	fibrils	fibril	NOUN
fnp-5497	64	10	(	(	PUNCT
fnp-5497	64	11	as	as	ADV
fnp-5497	64	12	low	low	ADJ
fnp-5497	64	13	as	as	ADP
fnp-5497	64	14	7.8	7.8	NUM
fnp-5497	64	15	 	 	SPACE
fnp-5497	64	16	ng	ng	PROPN
fnp-5497	64	17	)	)	PUNCT
fnp-5497	64	18	using	use	VERB
fnp-5497	64	19	affordable	affordable	ADJ
fnp-5497	64	20	materials	material	NOUN
fnp-5497	64	21	underscores	underscore	VERB
fnp-5497	64	22	its	its	PRON
fnp-5497	64	23	practicality	practicality	NOUN
fnp-5497	64	24	for	for	ADP
fnp-5497	64	25	widespread	widespread	ADJ
fnp-5497	64	26	application	application	NOUN
fnp-5497	64	27	.	.	PUNCT
fnp-5497	65	1	the	the	DET
fnp-5497	65	2	flexibility	flexibility	NOUN
fnp-5497	65	3	of	of	ADP
fnp-5497	65	4	the	the	DET
fnp-5497	65	5	method	method	NOUN
fnp-5497	65	6	,	,	PUNCT
fnp-5497	65	7	demonstrated	demonstrate	VERB
fnp-5497	65	8	by	by	ADP
fnp-5497	65	9	the	the	DET
fnp-5497	65	10	effective	effective	ADJ
fnp-5497	65	11	use	use	NOUN
fnp-5497	65	12	of	of	ADP
fnp-5497	65	13	different	different	ADJ
fnp-5497	65	14	solvents	solvent	NOUN
fnp-5497	65	15	like	like	ADP
fnp-5497	65	16	sarkosyl	sarkosyl	NOUN
fnp-5497	65	17	,	,	PUNCT
fnp-5497	65	18	adds	add	VERB
fnp-5497	65	19	to	to	ADP
fnp-5497	65	20	its	its	PRON
fnp-5497	65	21	appeal	appeal	NOUN
fnp-5497	65	22	in	in	ADP
fnp-5497	65	23	diverse	diverse	ADJ
fnp-5497	65	24	research	research	NOUN
fnp-5497	65	25	settings	setting	NOUN
fnp-5497	65	26	.	.	PUNCT
fnp-5497	66	1	this	this	DET
fnp-5497	66	2	study	study	NOUN
fnp-5497	66	3	therefore	therefore	ADV
fnp-5497	66	4	positions	position	VERB
fnp-5497	66	5	the	the	DET
fnp-5497	66	6	optimized	optimize	VERB
fnp-5497	66	7	fta	fta	PROPN
fnp-5497	66	8	protocol	protocol	NOUN
fnp-5497	66	9	as	as	ADP
fnp-5497	66	10	an	an	DET
fnp-5497	66	11	excellent	excellent	ADJ
fnp-5497	66	12	tool	tool	NOUN
fnp-5497	66	13	in	in	ADP
fnp-5497	66	14	the	the	DET
fnp-5497	66	15	arsenal	arsenal	NOUN
fnp-5497	66	16	of	of	ADP
fnp-5497	66	17	in	in	ADP
fnp-5497	66	18	vitro	vitro	X
fnp-5497	66	19	cell	cell	NOUN
fnp-5497	66	20	-	-	PUNCT
fnp-5497	66	21	free	free	ADJ
fnp-5497	66	22	tauopathy	tauopathy	NOUN
fnp-5497	66	23	research	research	NOUN
fnp-5497	66	24	,	,	PUNCT
fnp-5497	66	25	enabling	enable	VERB
fnp-5497	66	26	more	more	ADJ
fnp-5497	66	27	laboratories	laboratory	NOUN
fnp-5497	66	28	to	to	PART
fnp-5497	66	29	engage	engage	VERB
fnp-5497	66	30	in	in	ADP
fnp-5497	66	31	meaningful	meaningful	ADJ
fnp-5497	66	32	investigation	investigation	NOUN
fnp-5497	66	33	of	of	ADP
fnp-5497	66	34	tau	tau	PROPN
fnp-5497	66	35	aggregation	aggregation	NOUN
fnp-5497	66	36	.	.	PUNCT
fnp-5497	67	1	it	it	PRON
fnp-5497	67	2	paves	pave	VERB
fnp-5497	67	3	the	the	DET
fnp-5497	67	4	way	way	NOUN
fnp-5497	67	5	for	for	SCONJ
fnp-5497	67	6	the	the	DET
fnp-5497	67	7	use	use	NOUN
fnp-5497	67	8	of	of	ADP
fnp-5497	67	9	more	more	ADV
fnp-5497	67	10	intricate	intricate	ADJ
fnp-5497	67	11	techniques	technique	NOUN
fnp-5497	67	12	such	such	ADJ
fnp-5497	67	13	as	as	ADP
fnp-5497	67	14	em	em	PRON
fnp-5497	67	15	to	to	PART
fnp-5497	67	16	further	far	ADV
fnp-5497	67	17	elucidate	elucidate	VERB
fnp-5497	67	18	the	the	DET
fnp-5497	67	19	complex	complex	ADJ
fnp-5497	67	20	mechanisms	mechanism	NOUN
fnp-5497	67	21	underlying	underlie	VERB
fnp-5497	67	22	tauopathies	tauopathie	NOUN
fnp-5497	67	23	.	.	PUNCT
fnp-5497	68	1	by	by	ADP
fnp-5497	68	2	providing	provide	VERB
fnp-5497	68	3	a	a	DET
fnp-5497	68	4	cost	cost	NOUN
fnp-5497	68	5	-	-	PUNCT
fnp-5497	68	6	effective	effective	ADJ
fnp-5497	68	7	and	and	CCONJ
fnp-5497	68	8	user	user	NOUN
fnp-5497	68	9	-	-	PUNCT
fnp-5497	68	10	friendly	friendly	ADJ
fnp-5497	68	11	method	method	NOUN
fnp-5497	68	12	,	,	PUNCT
fnp-5497	68	13	we	we	PRON
fnp-5497	68	14	bridge	bridge	VERB
fnp-5497	68	15	the	the	DET
fnp-5497	68	16	gap	gap	NOUN
fnp-5497	68	17	between	between	ADP
fnp-5497	68	18	basic	basic	ADJ
fnp-5497	68	19	research	research	NOUN
fnp-5497	68	20	and	and	CCONJ
fnp-5497	68	21	advanced	advanced	ADJ
fnp-5497	68	22	structural	structural	ADJ
fnp-5497	68	23	analysis	analysis	NOUN
fnp-5497	68	24	,	,	PUNCT
fnp-5497	68	25	fostering	foster	VERB
fnp-5497	68	26	a	a	DET
fnp-5497	68	27	more	more	ADV
fnp-5497	68	28	comprehensive	comprehensive	ADJ
fnp-5497	68	29	understanding	understanding	NOUN
fnp-5497	68	30	of	of	ADP
fnp-5497	68	31	tauopathies	tauopathie	NOUN
fnp-5497	68	32	.	.	PUNCT
fnp-5497	69	1	author	author	NOUN
fnp-5497	69	2	contributions	contribution	NOUN
fnp-5497	69	3	conceptualization	conceptualization	NOUN
fnp-5497	69	4	,	,	PUNCT
fnp-5497	69	5	h.k	h.k	PROPN
fnp-5497	69	6	.	.	PROPN
fnp-5497	69	7	;	;	PUNCT
fnp-5497	69	8	software	software	NOUN
fnp-5497	69	9	,	,	PUNCT
fnp-5497	69	10	h.k	h.k	PROPN
fnp-5497	69	11	.	.	PROPN
fnp-5497	69	12	;	;	PUNCT
fnp-5497	69	13	investigation	investigation	NOUN
fnp-5497	69	14	,	,	PUNCT
fnp-5497	69	15	a.r.b	a.r.b	NOUN
fnp-5497	69	16	.	.	PROPN
fnp-5497	69	17	and	and	CCONJ
fnp-5497	69	18	h.k	h.k	PROPN
fnp-5497	69	19	.	.	PROPN
fnp-5497	69	20	;	;	PUNCT
fnp-5497	69	21	resources	resource	NOUN
fnp-5497	69	22	,	,	PUNCT
fnp-5497	69	23	h.k	h.k	PROPN
fnp-5497	69	24	.	.	PROPN
fnp-5497	69	25	;	;	PUNCT
fnp-5497	69	26	data	datum	NOUN
fnp-5497	69	27	curation	curation	NOUN
fnp-5497	69	28	,	,	PUNCT
fnp-5497	69	29	a.r.b	a.r.b	NOUN
fnp-5497	69	30	.	.	PROPN
fnp-5497	69	31	and	and	CCONJ
fnp-5497	69	32	h.k	h.k	PROPN
fnp-5497	69	33	.	.	PROPN
fnp-5497	69	34	;	;	PUNCT
fnp-5497	69	35	writing	write	VERB
fnp-5497	69	36	original	original	ADJ
fnp-5497	69	37	draft	draft	NOUN
fnp-5497	69	38	preparation	preparation	NOUN
fnp-5497	69	39	,	,	PUNCT
fnp-5497	69	40	h.k	h.k	PROPN
fnp-5497	69	41	.	.	PROPN
fnp-5497	69	42	;	;	PUNCT
fnp-5497	69	43	writing	write	VERB
fnp-5497	69	44	review	review	NOUN
fnp-5497	69	45	and	and	CCONJ
fnp-5497	69	46	editing	editing	NOUN
fnp-5497	69	47	,	,	PUNCT
fnp-5497	69	48	a.r.b	a.r.b	NOUN
fnp-5497	69	49	.	.	PROPN
fnp-5497	69	50	,	,	PUNCT
fnp-5497	69	51	t.l.r	t.l.r	PROPN
fnp-5497	69	52	.	.	PUNCT
fnp-5497	69	53	and	and	CCONJ
fnp-5497	69	54	h.k	h.k	PROPN
fnp-5497	69	55	.	.	PROPN
fnp-5497	69	56	;	;	PUNCT
fnp-5497	69	57	visualization	visualization	NOUN
fnp-5497	69	58	,	,	PUNCT
fnp-5497	69	59	h.k	h.k	PROPN
fnp-5497	69	60	.	.	PROPN
fnp-5497	69	61	;	;	PUNCT
fnp-5497	69	62	supervision	supervision	NOUN
fnp-5497	69	63	,	,	PUNCT
fnp-5497	69	64	h.k	h.k	PROPN
fnp-5497	69	65	.	.	PROPN
fnp-5497	69	66	;	;	PUNCT
fnp-5497	69	67	project	project	NOUN
fnp-5497	69	68	administration	administration	NOUN
fnp-5497	69	69	,	,	PUNCT
fnp-5497	69	70	h.k	h.k	PROPN
fnp-5497	69	71	.	.	PROPN
fnp-5497	69	72	;	;	PUNCT
fnp-5497	69	73	funding	funding	NOUN
fnp-5497	69	74	acquisition	acquisition	NOUN
fnp-5497	69	75	,	,	PUNCT
fnp-5497	69	76	t.l.r	t.l.r	NOUN
fnp-5497	69	77	.	.	PUNCT
fnp-5497	69	78	and	and	CCONJ
fnp-5497	69	79	h.k	h.k	PROPN
fnp-5497	69	80	.	.	PROPN
fnp-5497	69	81	acknowledgments	acknowledgment	NOUN
fnp-5497	69	82	we	we	PRON
fnp-5497	69	83	are	be	AUX
fnp-5497	69	84	grateful	grateful	ADJ
fnp-5497	69	85	to	to	ADP
fnp-5497	69	86	our	our	PRON
fnp-5497	69	87	colleagues	colleague	NOUN
fnp-5497	69	88	for	for	ADP
fnp-5497	69	89	helpful	helpful	ADJ
fnp-5497	69	90	discussions	discussion	NOUN
fnp-5497	69	91	and	and	CCONJ
fnp-5497	69	92	technical	technical	ADJ
fnp-5497	69	93	assistance	assistance	NOUN
fnp-5497	69	94	throughout	throughout	ADP
fnp-5497	69	95	the	the	DET
fnp-5497	69	96	course	course	NOUN
fnp-5497	69	97	of	of	ADP
fnp-5497	69	98	this	this	DET
fnp-5497	69	99	study	study	NOUN
fnp-5497	69	100	.	.	PUNCT
fnp-5497	70	1	this	this	DET
fnp-5497	70	2	work	work	NOUN
fnp-5497	70	3	was	be	AUX
fnp-5497	70	4	supported	support	VERB
fnp-5497	70	5	by	by	ADP
fnp-5497	70	6	the	the	DET
fnp-5497	70	7	national	national	PROPN
fnp-5497	70	8	institute	institute	PROPN
fnp-5497	70	9	on	on	ADP
fnp-5497	70	10	aging	aging	NOUN
fnp-5497	70	11	of	of	ADP
fnp-5497	70	12	the	the	DET
fnp-5497	70	13	national	national	PROPN
fnp-5497	70	14	institutes	institutes	PROPN
fnp-5497	70	15	of	of	ADP
fnp-5497	70	16	health	health	PROPN
fnp-5497	70	17	(	(	PUNCT
fnp-5497	70	18	nih	nih	PROPN
fnp-5497	70	19	)	)	PUNCT
fnp-5497	70	20	under	under	ADP
fnp-5497	70	21	award	award	NOUN
fnp-5497	70	22	number	number	NOUN
fnp-5497	70	23	r03	r03	NOUN
fnp-5497	70	24	 	 	SPACE
fnp-5497	70	25	ag072148	ag072148	PROPN
fnp-5497	70	26	.	.	PUNCT
fnp-5497	71	1	data	datum	NOUN
fnp-5497	71	2	availability	availability	NOUN
fnp-5497	71	3	statement	statement	NOUN
fnp-5497	71	4	the	the	DET
fnp-5497	71	5	datasets	dataset	NOUN
fnp-5497	71	6	generated	generate	VERB
fnp-5497	71	7	and/or	and/or	CCONJ
fnp-5497	71	8	analyzed	analyze	VERB
fnp-5497	71	9	during	during	ADP
fnp-5497	71	10	the	the	DET
fnp-5497	71	11	current	current	ADJ
fnp-5497	71	12	study	study	NOUN
fnp-5497	71	13	are	be	AUX
fnp-5497	71	14	available	available	ADJ
fnp-5497	71	15	on	on	ADP
fnp-5497	71	16	demand	demand	NOUN
fnp-5497	71	17	.	.	PUNCT
fnp-5497	72	1	conflicts	conflict	NOUN
fnp-5497	72	2	of	of	ADP
fnp-5497	72	3	interest	interest	NOUN
fnp-5497	72	4	statement	statement	NOUN
fnp-5497	72	5	the	the	DET
fnp-5497	72	6	authors	author	NOUN
fnp-5497	72	7	declare	declare	VERB
fnp-5497	72	8	no	no	DET
fnp-5497	72	9	conflicts	conflict	NOUN
fnp-5497	72	10	of	of	ADP
fnp-5497	72	11	interest	interest	NOUN
fnp-5497	72	12	.	.	PUNCT
fnp-5497	73	1	funding	funding	NOUN
fnp-5497	73	2	statement	statement	NOUN
fnp-5497	73	3	this	this	DET
fnp-5497	73	4	work	work	NOUN
fnp-5497	73	5	was	be	AUX
fnp-5497	73	6	supported	support	VERB
fnp-5497	73	7	by	by	ADP
fnp-5497	73	8	the	the	DET
fnp-5497	73	9	pilot	pilot	NOUN
fnp-5497	73	10	research	research	NOUN
fnp-5497	73	11	project	project	NOUN
fnp-5497	73	12	grant	grant	NOUN
fnp-5497	73	13	awarded	award	VERB
fnp-5497	73	14	by	by	ADP
fnp-5497	73	15	the	the	DET
fnp-5497	73	16	western	western	PROPN
fnp-5497	73	17	michigan	michigan	PROPN
fnp-5497	73	18	university	university	PROPN
fnp-5497	73	19	homer	homer	NOUN
fnp-5497	73	20	stryker	stryker	PROPN
fnp-5497	73	21	m.d	m.d	PROPN
fnp-5497	73	22	.	.	PROPN
fnp-5497	73	23	school	school	NOUN
fnp-5497	73	24	of	of	ADP
fnp-5497	73	25	medicine	medicine	NOUN
fnp-5497	73	26	,	,	PUNCT
fnp-5497	73	27	and	and	CCONJ
fnp-5497	73	28	public	public	ADJ
fnp-5497	73	29	health	health	NOUN
fnp-5497	73	30	service	service	NOUN
fnp-5497	73	31	grant	grant	NOUN
fnp-5497	73	32	ag072148	ag072148	PROPN
fnp-5497	73	33	awarded	award	VERB
fnp-5497	73	34	by	by	ADP
fnp-5497	73	35	the	the	DET
fnp-5497	73	36	national	national	PROPN
fnp-5497	73	37	institutes	institutes	PROPN
fnp-5497	73	38	of	of	ADP
fnp-5497	73	39	health	health	NOUN
fnp-5497	73	40	.	.	PUNCT
fnp-5497	74	1	references	reference	NOUN
fnp-5497	74	2	1	1	NUM
fnp-5497	74	3	.	.	PUNCT
fnp-5497	74	4	fitzpatrick	fitzpatrick	PROPN
fnp-5497	74	5	,	,	PUNCT
fnp-5497	75	1	a.	a.	PROPN
fnp-5497	75	2	w.	w.	PROPN
fnp-5497	75	3	p.	p.	PROPN
fnp-5497	75	4	et	et	PROPN
fnp-5497	76	1	al	al	PROPN
fnp-5497	76	2	.	.	PUNCT
fnp-5497	76	3	cryo	cryo	PROPN
fnp-5497	76	4	-	-	PUNCT
fnp-5497	76	5	em	em	PRON
fnp-5497	76	6	structures	structure	NOUN
fnp-5497	76	7	of	of	ADP
fnp-5497	76	8	tau	tau	PROPN
fnp-5497	76	9	filaments	filament	NOUN
fnp-5497	76	10	from	from	ADP
fnp-5497	76	11	alzheimer	alzheimer	PROPN
fnp-5497	76	12	's	's	PART
fnp-5497	76	13	disease	disease	NOUN
fnp-5497	76	14	.	.	PUNCT
fnp-5497	77	1	nature	nature	NOUN
fnp-5497	77	2	547	547	NUM
fnp-5497	77	3	,	,	PUNCT
fnp-5497	77	4	185	185	NUM
fnp-5497	77	5	-	-	SYM
fnp-5497	77	6	190	190	NUM
fnp-5497	77	7	,	,	PUNCT
fnp-5497	77	8	https://doi.org/10.1038/nature23002	https://doi.org/10.1038/nature23002	X
fnp-5497	77	9	(	(	PUNCT
fnp-5497	77	10	2017	2017	NUM
fnp-5497	77	11	)	)	PUNCT
fnp-5497	77	12	.	.	PUNCT
fnp-5497	78	1	2	2	X
fnp-5497	78	2	.	.	X
fnp-5497	78	3	friedhoff	friedhoff	NOUN
fnp-5497	78	4	,	,	PUNCT
fnp-5497	78	5	p.	p.	NOUN
fnp-5497	78	6	,	,	PUNCT
fnp-5497	78	7	schneider	schneider	PROPN
fnp-5497	78	8	,	,	PUNCT
fnp-5497	78	9	a.	a.	PROPN
fnp-5497	78	10	,	,	PUNCT
fnp-5497	78	11	mandelkow	mandelkow	PROPN
fnp-5497	78	12	,	,	PUNCT
fnp-5497	78	13	e.	e.	PROPN
fnp-5497	78	14	m.	m.	PROPN
fnp-5497	78	15	&	&	CCONJ
fnp-5497	78	16	mandelkow	mandelkow	PROPN
fnp-5497	78	17	,	,	PUNCT
fnp-5497	78	18	e.	e.	PROPN
fnp-5497	78	19	rapid	rapid	PROPN
fnp-5497	78	20	assembly	assembly	NOUN
fnp-5497	78	21	of	of	ADP
fnp-5497	78	22	alzheimer	alzheimer	PROPN
fnp-5497	78	23	-	-	PUNCT
fnp-5497	78	24	like	like	ADJ
fnp-5497	78	25	paired	pair	VERB
fnp-5497	78	26	helical	helical	ADJ
fnp-5497	78	27	filaments	filament	NOUN
fnp-5497	78	28	from	from	ADP
fnp-5497	78	29	microtubule	microtubule	NOUN
fnp-5497	78	30	-	-	PUNCT
fnp-5497	78	31	associated	associate	VERB
fnp-5497	78	32	protein	protein	NOUN
fnp-5497	78	33	tau	tau	PROPN
fnp-5497	78	34	monitored	monitor	VERB
fnp-5497	78	35	by	by	ADP
fnp-5497	78	36	fluorescence	fluorescence	NOUN
fnp-5497	78	37	in	in	ADP
fnp-5497	78	38	solution	solution	NOUN
fnp-5497	78	39	.	.	PUNCT
fnp-5497	79	1	biochemistry	biochemistry	NOUN
fnp-5497	79	2	37	37	NUM
fnp-5497	79	3	,	,	PUNCT
fnp-5497	79	4	10223	10223	NUM
fnp-5497	79	5	-	-	SYM
fnp-5497	79	6	10230	10230	NUM
fnp-5497	79	7	,	,	PUNCT
fnp-5497	79	8	https://doi.org/10.1021/bi980537d	https://doi.org/10.1021/bi980537d	X
fnp-5497	79	9	(	(	PUNCT
fnp-5497	79	10	1998	1998	NUM
fnp-5497	79	11	)	)	PUNCT
fnp-5497	79	12	.	.	PUNCT
fnp-5497	80	1	3	3	X
fnp-5497	80	2	.	.	X
fnp-5497	80	3	montgomery	montgomery	PROPN
fnp-5497	80	4	,	,	PUNCT
fnp-5497	80	5	k.	k.	PROPN
fnp-5497	81	1	m.	m.	PROPN
fnp-5497	81	2	et	et	PROPN
fnp-5497	81	3	al	al	PROPN
fnp-5497	81	4	.	.	PUNCT
fnp-5497	82	1	chemical	chemical	PROPN
fnp-5497	82	2	features	feature	NOUN
fnp-5497	82	3	of	of	ADP
fnp-5497	82	4	polyanions	polyanion	NOUN
fnp-5497	82	5	modulate	modulate	VERB
fnp-5497	82	6	tau	tau	PROPN
fnp-5497	82	7	aggregation	aggregation	NOUN
fnp-5497	82	8	and	and	CCONJ
fnp-5497	82	9	conformational	conformational	ADJ
fnp-5497	82	10	states	state	NOUN
fnp-5497	82	11	.	.	PUNCT
fnp-5497	83	1	j	j	PROPN
fnp-5497	83	2	am	be	AUX
fnp-5497	83	3	chem	chem	NOUN
fnp-5497	83	4	soc	soc	NOUN
fnp-5497	83	5	145	145	NUM
fnp-5497	83	6	,	,	PUNCT
fnp-5497	83	7	3926	3926	NUM
fnp-5497	83	8	-	-	SYM
fnp-5497	83	9	3936	3936	NUM
fnp-5497	83	10	,	,	PUNCT
fnp-5497	83	11	https://doi.org/10.1021/jacs.2c08004	https://doi.org/10.1021/jacs.2c08004	NOUN
fnp-5497	83	12	(	(	PUNCT
fnp-5497	83	13	2023	2023	NUM
fnp-5497	83	14	)	)	PUNCT
fnp-5497	83	15	.	.	PUNCT
fnp-5497	84	1	4	4	X
fnp-5497	84	2	.	.	X
fnp-5497	84	3	zhang	zhang	PROPN
fnp-5497	84	4	,	,	PUNCT
fnp-5497	84	5	w.	w.	PROPN
fnp-5497	84	6	et	et	PROPN
fnp-5497	84	7	al	al	PROPN
fnp-5497	84	8	.	.	PROPN
fnp-5497	84	9	heparin	heparin	NOUN
fnp-5497	84	10	-	-	PUNCT
fnp-5497	84	11	induced	induce	VERB
fnp-5497	84	12	tau	tau	PROPN
fnp-5497	84	13	filaments	filament	NOUN
fnp-5497	84	14	are	be	AUX
fnp-5497	84	15	polymorphic	polymorphic	ADJ
fnp-5497	84	16	and	and	CCONJ
fnp-5497	84	17	differ	differ	VERB
fnp-5497	84	18	from	from	ADP
fnp-5497	84	19	those	those	PRON
fnp-5497	84	20	in	in	ADP
fnp-5497	84	21	alzheimer	alzheimer	PROPN
fnp-5497	84	22	's	's	PART
fnp-5497	84	23	and	and	CCONJ
fnp-5497	84	24	pick	pick	NOUN
fnp-5497	84	25	's	's	PART
fnp-5497	84	26	diseases	disease	NOUN
fnp-5497	84	27	.	.	PUNCT
fnp-5497	85	1	elife	elife	NOUN
fnp-5497	85	2	8	8	NUM
fnp-5497	85	3	,	,	PUNCT
fnp-5497	85	4	https://doi.org/10.7554/elife.43584	https://doi.org/10.7554/elife.43584	NOUN
fnp-5497	85	5	(	(	PUNCT
fnp-5497	85	6	2019	2019	NUM
fnp-5497	85	7	)	)	PUNCT
fnp-5497	85	8	.	.	PUNCT
fnp-5497	86	1	5	5	X
fnp-5497	86	2	.	.	X
fnp-5497	86	3	al	al	PROPN
fnp-5497	86	4	-	-	PUNCT
fnp-5497	86	5	hilaly	hilaly	PROPN
fnp-5497	86	6	,	,	PUNCT
fnp-5497	86	7	y.	y.	PROPN
fnp-5497	86	8	k.	k.	PROPN
fnp-5497	86	9	et	et	PROPN
fnp-5497	87	1	al	al	PROPN
fnp-5497	87	2	.	.	PROPN
fnp-5497	88	1	tau	tau	PROPN
fnp-5497	88	2	(	(	PUNCT
fnp-5497	88	3	297	297	NUM
fnp-5497	88	4	-	-	SYM
fnp-5497	88	5	391	391	NUM
fnp-5497	88	6	)	)	PUNCT
fnp-5497	88	7	forms	form	VERB
fnp-5497	88	8	filaments	filament	NOUN
fnp-5497	88	9	that	that	PRON
fnp-5497	88	10	structurally	structurally	ADV
fnp-5497	88	11	mimic	mimic	VERB
fnp-5497	88	12	the	the	DET
fnp-5497	88	13	core	core	NOUN
fnp-5497	88	14	of	of	ADP
fnp-5497	88	15	paired	pair	VERB
fnp-5497	88	16	helical	helical	ADJ
fnp-5497	88	17	filaments	filament	NOUN
fnp-5497	88	18	in	in	ADP
fnp-5497	88	19	alzheimer	alzheimer	PROPN
fnp-5497	88	20	's	's	PART
fnp-5497	88	21	disease	disease	NOUN
fnp-5497	88	22	brain	brain	NOUN
fnp-5497	88	23	.	.	PUNCT
fnp-5497	89	1	febs	febs	PROPN
fnp-5497	89	2	lett	lett	PROPN
fnp-5497	89	3	594	594	NUM
fnp-5497	89	4	,	,	PUNCT
fnp-5497	89	5	944	944	NUM
fnp-5497	89	6	-	-	SYM
fnp-5497	89	7	950	950	NUM
fnp-5497	89	8	,	,	PUNCT
fnp-5497	89	9	https://doi.org/10.1002/1873-3468.13675	https://doi.org/10.1002/1873-3468.13675	PROPN
fnp-5497	89	10	(	(	PUNCT
fnp-5497	89	11	2020	2020	NUM
fnp-5497	89	12	)	)	PUNCT
fnp-5497	89	13	.	.	PUNCT
fnp-5497	90	1	6	6	X
fnp-5497	90	2	.	.	X
fnp-5497	90	3	lovestam	lovestam	PROPN
fnp-5497	90	4	,	,	PUNCT
fnp-5497	90	5	s.	s.	PROPN
fnp-5497	90	6	et	et	PROPN
fnp-5497	90	7	al	al	PROPN
fnp-5497	90	8	.	.	PROPN
fnp-5497	90	9	assembly	assembly	PROPN
fnp-5497	90	10	of	of	ADP
fnp-5497	90	11	recombinant	recombinant	ADJ
fnp-5497	90	12	tau	tau	PROPN
fnp-5497	90	13	into	into	ADP
fnp-5497	90	14	filaments	filament	NOUN
fnp-5497	90	15	identical	identical	ADJ
fnp-5497	90	16	to	to	ADP
fnp-5497	90	17	those	those	PRON
fnp-5497	90	18	of	of	ADP
fnp-5497	90	19	alzheimer	alzheimer	PROPN
fnp-5497	90	20	's	's	PART
fnp-5497	90	21	disease	disease	NOUN
fnp-5497	90	22	and	and	CCONJ
fnp-5497	90	23	chronic	chronic	ADJ
fnp-5497	90	24	traumatic	traumatic	ADJ
fnp-5497	90	25	encephalopathy	encephalopathy	NOUN
fnp-5497	90	26	.	.	PUNCT
fnp-5497	91	1	elife	elife	NOUN
fnp-5497	91	2	11	11	NUM
fnp-5497	91	3	,	,	PUNCT
fnp-5497	91	4	https://doi.org/10.7554/elife.76494	https://doi.org/10.7554/elife.76494	X
fnp-5497	91	5	(	(	PUNCT
fnp-5497	91	6	2022	2022	NUM
fnp-5497	91	7	)	)	PUNCT
fnp-5497	91	8	.	.	PUNCT
fnp-5497	92	1	7	7	X
fnp-5497	92	2	.	.	X
fnp-5497	92	3	chang	chang	PROPN
fnp-5497	92	4	,	,	PUNCT
fnp-5497	92	5	e.	e.	PROPN
fnp-5497	92	6	&	&	CCONJ
fnp-5497	92	7	kuret	kuret	PROPN
fnp-5497	92	8	,	,	PUNCT
fnp-5497	92	9	j.	j.	PROPN
fnp-5497	92	10	detection	detection	PROPN
fnp-5497	92	11	and	and	CCONJ
fnp-5497	92	12	quantification	quantification	NOUN
fnp-5497	92	13	of	of	ADP
fnp-5497	92	14	tau	tau	PROPN
fnp-5497	92	15	aggregation	aggregation	NOUN
fnp-5497	92	16	using	use	VERB
fnp-5497	92	17	a	a	DET
fnp-5497	92	18	membrane	membrane	NOUN
fnp-5497	92	19	filter	filter	NOUN
fnp-5497	92	20	assay	assay	NOUN
fnp-5497	92	21	.	.	PUNCT
fnp-5497	93	1	anal	anal	ADJ
fnp-5497	93	2	biochem	biochem	NOUN
fnp-5497	93	3	373	373	NUM
fnp-5497	93	4	,	,	PUNCT
fnp-5497	93	5	330	330	NUM
fnp-5497	93	6	-	-	SYM
fnp-5497	93	7	336	336	NUM
fnp-5497	93	8	,	,	PUNCT
fnp-5497	93	9	https://doi.org/10.1016/j.ab.2007.09.015	https://doi.org/10.1016/j.ab.2007.09.015	PROPN
fnp-5497	93	10	(	(	PUNCT
fnp-5497	93	11	2008	2008	NUM
fnp-5497	93	12	)	)	PUNCT
fnp-5497	93	13	.	.	PUNCT
fnp-5497	94	1	8	8	X
fnp-5497	94	2	.	.	X
fnp-5497	94	3	greenberg	greenberg	PROPN
fnp-5497	94	4	,	,	PUNCT
fnp-5497	94	5	s.	s.	PROPN
fnp-5497	94	6	g.	g.	PROPN
fnp-5497	94	7	&	&	CCONJ
fnp-5497	94	8	davies	davy	NOUN
fnp-5497	94	9	,	,	PUNCT
fnp-5497	94	10	p.	p.	NOUN
fnp-5497	94	11	a	a	DET
fnp-5497	94	12	preparation	preparation	NOUN
fnp-5497	94	13	of	of	ADP
fnp-5497	94	14	alzheimer	alzheimer	PROPN
fnp-5497	94	15	paired	pair	VERB
fnp-5497	94	16	helical	helical	ADJ
fnp-5497	94	17	filaments	filament	NOUN
fnp-5497	94	18	that	that	PRON
fnp-5497	94	19	displays	display	VERB
fnp-5497	94	20	distinct	distinct	ADJ
fnp-5497	94	21	tau	tau	NOUN
fnp-5497	94	22	proteins	protein	NOUN
fnp-5497	94	23	by	by	ADP
fnp-5497	94	24	polyacrylamide	polyacrylamide	ADJ
fnp-5497	94	25	gel	gel	NOUN
fnp-5497	94	26	electrophoresis	electrophoresis	NOUN
fnp-5497	94	27	.	.	PUNCT
fnp-5497	95	1	proc	proc	PROPN
fnp-5497	95	2	natl	natl	PROPN
fnp-5497	95	3	acad	acad	PROPN
fnp-5497	95	4	sci	sci	PROPN
fnp-5497	95	5	u	u	PROPN
fnp-5497	95	6	 	 	SPACE
fnp-5497	95	7	s	s	VERB
fnp-5497	95	8	 	 	SPACE
fnp-5497	95	9	a	a	DET
fnp-5497	95	10	87	87	NUM
fnp-5497	95	11	,	,	PUNCT
fnp-5497	95	12	5827	5827	NUM
fnp-5497	95	13	-	-	SYM
fnp-5497	95	14	5831	5831	NUM
fnp-5497	95	15	,	,	PUNCT
fnp-5497	95	16	https://doi.org/10.1073/pnas.87.15.5827	https://doi.org/10.1073/pnas.87.15.5827	PROPN
fnp-5497	95	17	(	(	PUNCT
fnp-5497	95	18	1990	1990	NUM
fnp-5497	95	19	)	)	PUNCT
fnp-5497	95	20	.	.	PUNCT
fnp-5497	96	1	9	9	X
fnp-5497	96	2	.	.	X
fnp-5497	96	3	desantis	desantis	PROPN
fnp-5497	96	4	,	,	PUNCT
fnp-5497	96	5	m.	m.	PROPN
fnp-5497	97	1	e.	e.	PROPN
fnp-5497	97	2	et	et	PROPN
fnp-5497	97	3	al	al	PROPN
fnp-5497	97	4	.	.	PROPN
fnp-5497	98	1	operational	operational	ADJ
fnp-5497	98	2	plasticity	plasticity	NOUN
fnp-5497	98	3	enables	enable	VERB
fnp-5497	98	4	hsp104	hsp104	PROPN
fnp-5497	98	5	to	to	PART
fnp-5497	98	6	disaggregate	disaggregate	VERB
fnp-5497	98	7	diverse	diverse	ADJ
fnp-5497	98	8	amyloid	amyloid	NOUN
fnp-5497	98	9	and	and	CCONJ
fnp-5497	98	10	nonamyloid	nonamyloid	ADJ
fnp-5497	98	11	clients	client	NOUN
fnp-5497	98	12	.	.	PUNCT
fnp-5497	99	1	cell	cell	NOUN
fnp-5497	99	2	151	151	NUM
fnp-5497	99	3	,	,	PUNCT
fnp-5497	99	4	778	778	NUM
fnp-5497	99	5	-	-	SYM
fnp-5497	99	6	793	793	NUM
fnp-5497	99	7	,	,	PUNCT
fnp-5497	99	8	https://doi.org/10.1016/j.cell.2012.09.038	https://doi.org/10.1016/j.cell.2012.09.038	NUM
fnp-5497	99	9	(	(	PUNCT
fnp-5497	99	10	2012	2012	NUM
fnp-5497	99	11	)	)	PUNCT
fnp-5497	99	12	.	.	PUNCT
fnp-5497	100	1	10	10	NUM
fnp-5497	100	2	.	.	X
fnp-5497	100	3	gasteiger	gasteiger	NOUN
fnp-5497	100	4	,	,	PUNCT
fnp-5497	100	5	e.	e.	PROPN
fnp-5497	100	6	et	et	PROPN
fnp-5497	100	7	al	al	PROPN
fnp-5497	100	8	.	.	PROPN
fnp-5497	100	9	expasy	expasy	PROPN
fnp-5497	100	10	:	:	PUNCT
fnp-5497	100	11	the	the	DET
fnp-5497	100	12	proteomics	proteomic	NOUN
fnp-5497	100	13	server	server	NOUN
fnp-5497	100	14	for	for	ADP
fnp-5497	100	15	in	in	ADP
fnp-5497	100	16	-	-	PUNCT
fnp-5497	100	17	depth	depth	NOUN
fnp-5497	100	18	protein	protein	NOUN
fnp-5497	100	19	knowledge	knowledge	NOUN
fnp-5497	100	20	and	and	CCONJ
fnp-5497	100	21	analysis	analysis	NOUN
fnp-5497	100	22	.	.	PUNCT
fnp-5497	101	1	nucleic	nucleic	ADJ
fnp-5497	101	2	acids	acid	NOUN
fnp-5497	101	3	res	re	NOUN
fnp-5497	101	4	31	31	NUM
fnp-5497	101	5	,	,	PUNCT
fnp-5497	101	6	3784	3784	NUM
fnp-5497	101	7	-	-	SYM
fnp-5497	101	8	3788	3788	NUM
fnp-5497	101	9	,	,	PUNCT
fnp-5497	101	10	https://doi.org/10.1093/nar/gkg563	https://doi.org/10.1093/nar/gkg563	PROPN
fnp-5497	101	11	(	(	PUNCT
fnp-5497	101	12	2003	2003	NUM
fnp-5497	101	13	)	)	PUNCT
fnp-5497	101	14	.	.	PUNCT
fnp-5497	102	1	copyright	copyright	NOUN
fnp-5497	102	2	:	:	PUNCT
fnp-5497	102	3	©	©	PROPN
fnp-5497	102	4	2024	2024	NUM
fnp-5497	102	5	the	the	DET
fnp-5497	102	6	author(s	author(s	NOUN
fnp-5497	102	7	)	)	PUNCT
fnp-5497	102	8	.	.	PUNCT
fnp-5497	103	1	this	this	PRON
fnp-5497	103	2	is	be	AUX
fnp-5497	103	3	an	an	DET
fnp-5497	103	4	open	open	ADJ
fnp-5497	103	5	access	access	NOUN
fnp-5497	103	6	article	article	NOUN
fnp-5497	103	7	distributed	distribute	VERB
fnp-5497	103	8	under	under	ADP
fnp-5497	103	9	the	the	DET
fnp-5497	103	10	terms	term	NOUN
fnp-5497	103	11	of	of	ADP
fnp-5497	103	12	the	the	DET
fnp-5497	103	13	creative	creative	ADJ
fnp-5497	103	14	commons	common	NOUN
fnp-5497	103	15	attribution	attribution	NOUN
fnp-5497	103	16	4.0	4.0	NUM
fnp-5497	103	17	international	international	ADJ
fnp-5497	103	18	license	license	NOUN
fnp-5497	103	19	(	(	PUNCT
fnp-5497	103	20	https://creativecommons.org/licenses/by/4.0/	https://creativecommons.org/licenses/by/4.0/	PROPN
fnp-5497	103	21	)	)	PUNCT
fnp-5497	103	22	,	,	PUNCT
fnp-5497	103	23	which	which	PRON
fnp-5497	103	24	permits	permit	VERB
fnp-5497	103	25	unrestricted	unrestricted	ADJ
fnp-5497	103	26	use	use	NOUN
fnp-5497	103	27	,	,	PUNCT
fnp-5497	103	28	distribution	distribution	NOUN
fnp-5497	103	29	,	,	PUNCT
fnp-5497	103	30	and	and	CCONJ
fnp-5497	103	31	reproduction	reproduction	NOUN
fnp-5497	103	32	in	in	ADP
fnp-5497	103	33	any	any	DET
fnp-5497	103	34	medium	medium	NOUN
fnp-5497	103	35	,	,	PUNCT
fnp-5497	103	36	provided	provide	VERB
fnp-5497	103	37	the	the	DET
fnp-5497	103	38	original	original	ADJ
fnp-5497	103	39	author	author	NOUN
fnp-5497	103	40	and	and	CCONJ
fnp-5497	103	41	source	source	NOUN
fnp-5497	103	42	are	be	AUX
fnp-5497	103	43	credited	credit	VERB
fnp-5497	103	44	,	,	PUNCT
fnp-5497	103	45	a	a	DET
fnp-5497	103	46	link	link	NOUN
fnp-5497	103	47	to	to	ADP
fnp-5497	103	48	the	the	DET
fnp-5497	103	49	creative	creative	ADJ
fnp-5497	103	50	commons	common	NOUN
fnp-5497	103	51	license	license	NOUN
fnp-5497	103	52	is	be	AUX
fnp-5497	103	53	provided	provide	VERB
fnp-5497	103	54	,	,	PUNCT
fnp-5497	103	55	and	and	CCONJ
fnp-5497	103	56	any	any	DET
fnp-5497	103	57	changes	change	NOUN
fnp-5497	103	58	are	be	AUX
fnp-5497	103	59	indicated	indicate	VERB
fnp-5497	103	60	.	.	PUNCT
fnp-5497	104	1	the	the	DET
fnp-5497	104	2	creative	creative	ADJ
fnp-5497	104	3	commons	common	NOUN
fnp-5497	104	4	public	public	ADJ
fnp-5497	104	5	domain	domain	NOUN
fnp-5497	104	6	dedication	dedication	NOUN
fnp-5497	104	7	waiver	waiver	NOUN
fnp-5497	104	8	(	(	PUNCT
fnp-5497	104	9	https://creativecommons.org/publicdomain/zero/1.0/	https://creativecommons.org/publicdomain/zero/1.0/	PROPN
fnp-5497	104	10	)	)	PUNCT
fnp-5497	104	11	applies	apply	VERB
fnp-5497	104	12	to	to	ADP
fnp-5497	104	13	the	the	DET
fnp-5497	104	14	data	datum	NOUN
fnp-5497	104	15	made	make	VERB
fnp-5497	104	16	available	available	ADJ
fnp-5497	104	17	in	in	ADP
fnp-5497	104	18	this	this	DET
fnp-5497	104	19	article	article	NOUN
fnp-5497	104	20	,	,	PUNCT
fnp-5497	104	21	unless	unless	SCONJ
fnp-5497	104	22	otherwise	otherwise	ADV
fnp-5497	104	23	stated	state	VERB
fnp-5497	104	24	.	.	PUNCT