id	sid	tid	token	lemma	pos
fnp-5883	1	1	cryopreservation	cryopreservation	NOUN
fnp-5883	1	2	of	of	ADP
fnp-5883	1	3	brain	brain	NOUN
fnp-5883	1	4	cell	cell	NOUN
fnp-5883	1	5	structure	structure	NOUN
fnp-5883	1	6	:	:	PUNCT
fnp-5883	1	7	a	a	DET
fnp-5883	1	8	review	review	NOUN
fnp-5883	1	9	feel	feel	VERB
fnp-5883	1	10	free	free	ADJ
fnp-5883	1	11	to	to	PART
fnp-5883	1	12	add	add	VERB
fnp-5883	1	13	comments	comment	NOUN
fnp-5883	1	14	by	by	ADP
fnp-5883	1	15	clicking	click	VERB
fnp-5883	1	16	these	these	DET
fnp-5883	1	17	icons	icon	NOUN
fnp-5883	1	18	on	on	ADP
fnp-5883	1	19	the	the	DET
fnp-5883	1	20	sidebar	sidebar	NOUN
fnp-5883	1	21	free	free	ADJ
fnp-5883	1	22	neuropathology	neuropathology	NOUN
fnp-5883	1	23	5:35	5:35	NUM
fnp-5883	1	24	(	(	PUNCT
fnp-5883	1	25	2024	2024	NUM
fnp-5883	1	26	)	)	PUNCT
fnp-5883	1	27	review	review	NOUN
fnp-5883	1	28	cryopreservation	cryopreservation	NOUN
fnp-5883	1	29	of	of	ADP
fnp-5883	1	30	brain	brain	NOUN
fnp-5883	1	31	cell	cell	NOUN
fnp-5883	1	32	structure	structure	NOUN
fnp-5883	1	33	:	:	PUNCT
fnp-5883	1	34	a	a	DET
fnp-5883	1	35	review	review	NOUN
fnp-5883	1	36	andrew	andrew	PROPN
fnp-5883	1	37	t.	t.	PROPN
fnp-5883	1	38	mckenzie1	mckenzie1	PROPN
fnp-5883	1	39	,	,	PUNCT
fnp-5883	1	40	emma	emma	PROPN
fnp-5883	1	41	l.	l.	PROPN
fnp-5883	1	42	thorn2,3	thorn2,3	PROPN
fnp-5883	1	43	,	,	PUNCT
fnp-5883	1	44	oge	oge	PROPN
fnp-5883	1	45	nnadi4	nnadi4	PROPN
fnp-5883	1	46	,	,	PUNCT
fnp-5883	1	47	borys	borys	PROPN
fnp-5883	1	48	wróbel5,6	wróbel5,6	PROPN
fnp-5883	1	49	,	,	PUNCT
fnp-5883	1	50	emil	emil	PROPN
fnp-5883	1	51	kendziorra7	kendziorra7	PROPN
fnp-5883	1	52	,	,	PUNCT
fnp-5883	1	53	kurt	kurt	PROPN
fnp-5883	1	54	farrell2,3	farrell2,3	PROPN
fnp-5883	1	55	,	,	PUNCT
fnp-5883	1	56	john	john	PROPN
fnp-5883	1	57	f.	f.	PROPN
fnp-5883	1	58	crary2,3	crary2,3	PROPN
fnp-5883	1	59	apex	apex	NOUN
fnp-5883	1	60	neuroscience	neuroscience	NOUN
fnp-5883	1	61	,	,	PUNCT
fnp-5883	1	62	salem	salem	NOUN
fnp-5883	1	63	,	,	PUNCT
fnp-5883	1	64	oregon	oregon	PROPN
fnp-5883	1	65	,	,	PUNCT
fnp-5883	1	66	usa	usa	PROPN
fnp-5883	1	67	friedman	friedman	PROPN
fnp-5883	1	68	brain	brain	PROPN
fnp-5883	1	69	institute	institute	PROPN
fnp-5883	1	70	,	,	PUNCT
fnp-5883	1	71	departments	department	NOUN
fnp-5883	1	72	of	of	ADP
fnp-5883	1	73	pathology	pathology	NOUN
fnp-5883	1	74	,	,	PUNCT
fnp-5883	1	75	neuroscience	neuroscience	NOUN
fnp-5883	1	76	,	,	PUNCT
fnp-5883	1	77	and	and	CCONJ
fnp-5883	1	78	artificial	artificial	ADJ
fnp-5883	1	79	intelligence	intelligence	NOUN
fnp-5883	1	80	&	&	CCONJ
fnp-5883	1	81	human	human	PROPN
fnp-5883	1	82	health	health	PROPN
fnp-5883	1	83	,	,	PUNCT
fnp-5883	1	84	icahn	icahn	PROPN
fnp-5883	1	85	school	school	PROPN
fnp-5883	1	86	of	of	ADP
fnp-5883	1	87	medicine	medicine	NOUN
fnp-5883	1	88	at	at	ADP
fnp-5883	1	89	mount	mount	PROPN
fnp-5883	1	90	sinai	sinai	PROPN
fnp-5883	1	91	,	,	PUNCT
fnp-5883	1	92	new	new	PROPN
fnp-5883	1	93	york	york	PROPN
fnp-5883	1	94	,	,	PUNCT
fnp-5883	1	95	new	new	PROPN
fnp-5883	1	96	york	york	PROPN
fnp-5883	1	97	,	,	PUNCT
fnp-5883	1	98	usa	usa	PROPN
fnp-5883	1	99	neuropathology	neuropathology	PROPN
fnp-5883	1	100	brain	brain	PROPN
fnp-5883	1	101	bank	bank	PROPN
fnp-5883	1	102	&	&	CCONJ
fnp-5883	1	103	research	research	PROPN
fnp-5883	1	104	core	core	PROPN
fnp-5883	1	105	and	and	CCONJ
fnp-5883	1	106	ronald	ronald	PROPN
fnp-5883	1	107	m.	m.	PROPN
fnp-5883	1	108	loeb	loeb	PROPN
fnp-5883	1	109	center	center	PROPN
fnp-5883	1	110	for	for	ADP
fnp-5883	1	111	alzheimer	alzheimer	PROPN
fnp-5883	1	112	's	's	PART
fnp-5883	1	113	disease	disease	NOUN
fnp-5883	1	114	,	,	PUNCT
fnp-5883	1	115	icahn	icahn	PROPN
fnp-5883	1	116	school	school	PROPN
fnp-5883	1	117	of	of	ADP
fnp-5883	1	118	medicine	medicine	NOUN
fnp-5883	1	119	at	at	ADP
fnp-5883	1	120	mount	mount	PROPN
fnp-5883	1	121	sinai	sinai	PROPN
fnp-5883	1	122	,	,	PUNCT
fnp-5883	1	123	new	new	PROPN
fnp-5883	1	124	york	york	PROPN
fnp-5883	1	125	,	,	PUNCT
fnp-5883	1	126	new	new	PROPN
fnp-5883	1	127	york	york	PROPN
fnp-5883	1	128	,	,	PUNCT
fnp-5883	1	129	usa	usa	PROPN
fnp-5883	1	130	brain	brain	PROPN
fnp-5883	1	131	preservation	preservation	PROPN
fnp-5883	1	132	foundation	foundation	PROPN
fnp-5883	1	133	,	,	PUNCT
fnp-5883	1	134	ashburn	ashburn	PROPN
fnp-5883	1	135	,	,	PUNCT
fnp-5883	1	136	virginia	virginia	PROPN
fnp-5883	1	137	,	,	PUNCT
fnp-5883	1	138	usa	usa	PROPN
fnp-5883	1	139	european	european	PROPN
fnp-5883	1	140	institute	institute	PROPN
fnp-5883	1	141	for	for	ADP
fnp-5883	1	142	brain	brain	NOUN
fnp-5883	1	143	research	research	NOUN
fnp-5883	1	144	,	,	PUNCT
fnp-5883	1	145	amstelveen	amstelveen	VERB
fnp-5883	1	146	,	,	PUNCT
fnp-5883	1	147	the	the	DET
fnp-5883	1	148	netherlands	netherlands	PROPN
fnp-5883	1	149	biopreservation	biopreservation	PROPN
fnp-5883	1	150	institute	institute	PROPN
fnp-5883	1	151	,	,	PUNCT
fnp-5883	1	152	vancouver	vancouver	PROPN
fnp-5883	1	153	,	,	PUNCT
fnp-5883	1	154	washington	washington	PROPN
fnp-5883	1	155	,	,	PUNCT
fnp-5883	1	156	usa	usa	PROPN
fnp-5883	1	157	european	european	PROPN
fnp-5883	1	158	biostasis	biostasis	PROPN
fnp-5883	1	159	foundation	foundation	PROPN
fnp-5883	1	160	,	,	PUNCT
fnp-5883	1	161	riehen	riehen	PROPN
fnp-5883	1	162	,	,	PUNCT
fnp-5883	1	163	canton	canton	PROPN
fnp-5883	1	164	of	of	ADP
fnp-5883	1	165	basel	basel	PROPN
fnp-5883	1	166	-	-	PUNCT
fnp-5883	1	167	stadt	stadt	NOUN
fnp-5883	1	168	,	,	PUNCT
fnp-5883	1	169	switzerland	switzerland	PROPN
fnp-5883	1	170	corresponding	correspond	VERB
fnp-5883	1	171	authors	author	NOUN
fnp-5883	1	172	:	:	PUNCT
fnp-5883	1	173	andrew	andrew	PROPN
fnp-5883	1	174	t.	t.	PROPN
fnp-5883	1	175	mckenzie	mckenzie	PROPN
fnp-5883	1	176	·	·	PUNCT
fnp-5883	1	177	apex	apex	VERB
fnp-5883	1	178	neuroscience	neuroscience	NOUN
fnp-5883	1	179	·	·	PUNCT
fnp-5883	1	180	3265	3265	NUM
fnp-5883	1	181	marietta	marietta	PROPN
fnp-5883	1	182	st	st	PROPN
fnp-5883	1	183	se	se	PROPN
fnp-5883	1	184	·	·	PUNCT
fnp-5883	1	185	salem	salem	PROPN
fnp-5883	1	186	·	·	PUNCT
fnp-5883	1	187	or	or	CCONJ
fnp-5883	1	188	97317	97317	NUM
fnp-5883	1	189	·	·	PUNCT
fnp-5883	1	190	usa	usa	PROPN
fnp-5883	1	191	amckenzie@apexneuro.org	amckenzie@apexneuro.org	PROPN
fnp-5883	1	192	john	john	PROPN
fnp-5883	1	193	f.	f.	PROPN
fnp-5883	1	194	crary	crary	PROPN
fnp-5883	1	195	·	·	PUNCT
fnp-5883	1	196	friedman	friedman	PROPN
fnp-5883	1	197	brain	brain	PROPN
fnp-5883	1	198	institute	institute	PROPN
fnp-5883	1	199	·	·	PUNCT
fnp-5883	1	200	departments	department	NOUN
fnp-5883	1	201	of	of	ADP
fnp-5883	1	202	pathology	pathology	NOUN
fnp-5883	1	203	,	,	PUNCT
fnp-5883	1	204	neuroscience	neuroscience	NOUN
fnp-5883	1	205	,	,	PUNCT
fnp-5883	1	206	and	and	CCONJ
fnp-5883	1	207	artificial	artificial	ADJ
fnp-5883	1	208	intelligence	intelligence	NOUN
fnp-5883	1	209	&	&	CCONJ
fnp-5883	1	210	human	human	PROPN
fnp-5883	1	211	health	health	PROPN
fnp-5883	1	212	,	,	PUNCT
fnp-5883	1	213	icahn	icahn	PROPN
fnp-5883	1	214	school	school	PROPN
fnp-5883	1	215	of	of	ADP
fnp-5883	1	216	medicine	medicine	NOUN
fnp-5883	1	217	at	at	ADP
fnp-5883	1	218	mount	mount	PROPN
fnp-5883	1	219	sinai	sinai	PROPN
fnp-5883	1	220	·	·	PUNCT
fnp-5883	1	221	1	1	NUM
fnp-5883	1	222	gustave	gustave	X
fnp-5883	1	223	l.	l.	NOUN
fnp-5883	1	224	levy	levy	PROPN
fnp-5883	1	225	place	place	NOUN
fnp-5883	1	226	·	·	PUNCT
fnp-5883	1	227	box	box	NOUN
fnp-5883	1	228	1194	1194	NUM
fnp-5883	1	229	·	·	PUNCT
fnp-5883	1	230	new	new	PROPN
fnp-5883	1	231	york	york	PROPN
fnp-5883	1	232	·	·	PUNCT
fnp-5883	1	233	ny	ny	PROPN
fnp-5883	1	234	10029	10029	NUM
fnp-5883	1	235	·	·	PUNCT
fnp-5883	1	236	usa	usa	PROPN
fnp-5883	1	237	john.crary@mountsinai.org	john.crary@mountsinai.org	ADP
fnp-5883	1	238	additional	additional	ADJ
fnp-5883	1	239	resources	resource	NOUN
fnp-5883	1	240	and	and	CCONJ
fnp-5883	1	241	electronic	electronic	ADJ
fnp-5883	1	242	supplementary	supplementary	ADJ
fnp-5883	1	243	material	material	NOUN
fnp-5883	1	244	:	:	PUNCT
fnp-5883	1	245	supplementary	supplementary	ADJ
fnp-5883	1	246	material	material	NOUN
fnp-5883	1	247	submitted	submit	VERB
fnp-5883	1	248	:	:	PUNCT
fnp-5883	1	249	11	11	NUM
fnp-5883	1	250	september	september	PROPN
fnp-5883	1	251	2024	2024	NUM
fnp-5883	1	252	accepted	accept	VERB
fnp-5883	1	253	:	:	PUNCT
fnp-5883	1	254	19	19	NUM
fnp-5883	1	255	october	october	PROPN
fnp-5883	1	256	2024	2024	NUM
fnp-5883	1	257	copyedited	copyedit	VERB
fnp-5883	1	258	by	by	ADP
fnp-5883	1	259	:	:	PUNCT
fnp-5883	1	260	georg	georg	NOUN
fnp-5883	1	261	haase	haase	NOUN
fnp-5883	1	262	published	publish	VERB
fnp-5883	1	263	:	:	PUNCT
fnp-5883	1	264	11	11	NUM
fnp-5883	1	265	december	december	PROPN
fnp-5883	1	266	2024	2024	NUM
fnp-5883	1	267	https://doi.org/10.17879/freeneuropathology-2024-5883	https://doi.org/10.17879/freeneuropathology-2024-5883	NOUN
fnp-5883	1	268	keywords	keyword	VERB
fnp-5883	1	269	:	:	PUNCT
fnp-5883	1	270	cryopreservation	cryopreservation	NOUN
fnp-5883	1	271	,	,	PUNCT
fnp-5883	1	272	vitrification	vitrification	NOUN
fnp-5883	1	273	,	,	PUNCT
fnp-5883	1	274	brain	brain	NOUN
fnp-5883	1	275	banking	banking	NOUN
fnp-5883	1	276	,	,	PUNCT
fnp-5883	1	277	cryoprotectant	cryoprotectant	NOUN
fnp-5883	1	278	,	,	PUNCT
fnp-5883	1	279	cryofixation	cryofixation	NOUN
fnp-5883	1	280	,	,	PUNCT
fnp-5883	1	281	ice	ice	NOUN
fnp-5883	1	282	artifact	artifact	ADJ
fnp-5883	1	283	abstract	abstract	ADJ
fnp-5883	1	284	cryopreservation	cryopreservation	NOUN
fnp-5883	1	285	,	,	PUNCT
fnp-5883	1	286	the	the	DET
fnp-5883	1	287	preservation	preservation	NOUN
fnp-5883	1	288	of	of	ADP
fnp-5883	1	289	tissues	tissue	NOUN
fnp-5883	1	290	at	at	ADP
fnp-5883	1	291	subzero	subzero	NOUN
fnp-5883	1	292	temperatures	temperature	NOUN
fnp-5883	1	293	,	,	PUNCT
fnp-5883	1	294	is	be	AUX
fnp-5883	1	295	a	a	DET
fnp-5883	1	296	mainstay	mainstay	NOUN
fnp-5883	1	297	of	of	ADP
fnp-5883	1	298	brain	brain	NOUN
fnp-5883	1	299	banking	banking	NOUN
fnp-5883	1	300	that	that	PRON
fnp-5883	1	301	allows	allow	VERB
fnp-5883	1	302	for	for	ADP
fnp-5883	1	303	the	the	DET
fnp-5883	1	304	storage	storage	NOUN
fnp-5883	1	305	of	of	ADP
fnp-5883	1	306	brain	brain	NOUN
fnp-5883	1	307	tissue	tissue	NOUN
fnp-5883	1	308	without	without	ADP
fnp-5883	1	309	the	the	DET
fnp-5883	1	310	use	use	NOUN
fnp-5883	1	311	of	of	ADP
fnp-5883	1	312	chemical	chemical	ADJ
fnp-5883	1	313	fixatives	fixative	NOUN
fnp-5883	1	314	.	.	PUNCT
fnp-5883	2	1	this	this	PRON
fnp-5883	2	2	is	be	AUX
fnp-5883	2	3	particularly	particularly	ADV
fnp-5883	2	4	important	important	ADJ
fnp-5883	2	5	for	for	ADP
fnp-5883	2	6	molecular	molecular	ADJ
fnp-5883	2	7	studies	study	NOUN
fnp-5883	2	8	that	that	PRON
fnp-5883	2	9	are	be	AUX
fnp-5883	2	10	incompatible	incompatible	ADJ
fnp-5883	2	11	with	with	ADP
fnp-5883	2	12	tissue	tissue	NOUN
fnp-5883	2	13	fixation	fixation	NOUN
fnp-5883	2	14	.	.	PUNCT
fnp-5883	3	1	however	however	ADV
fnp-5883	3	2	,	,	PUNCT
fnp-5883	3	3	brain	brain	NOUN
fnp-5883	3	4	tissue	tissue	NOUN
fnp-5883	3	5	is	be	AUX
fnp-5883	3	6	vulnerable	vulnerable	ADJ
fnp-5883	3	7	to	to	ADP
fnp-5883	3	8	various	various	ADJ
fnp-5883	3	9	forms	form	NOUN
fnp-5883	3	10	of	of	ADP
fnp-5883	3	11	damage	damage	NOUN
fnp-5883	3	12	during	during	ADP
fnp-5883	3	13	the	the	DET
fnp-5883	3	14	cryopreservation	cryopreservation	NOUN
fnp-5883	3	15	process	process	NOUN
fnp-5883	3	16	,	,	PUNCT
fnp-5883	3	17	in	in	ADP
fnp-5883	3	18	particular	particular	ADJ
fnp-5883	3	19	due	due	ADP
fnp-5883	3	20	to	to	ADP
fnp-5883	3	21	the	the	DET
fnp-5883	3	22	phase	phase	NOUN
fnp-5883	3	23	transition	transition	NOUN
fnp-5883	3	24	of	of	ADP
fnp-5883	3	25	water	water	NOUN
fnp-5883	3	26	from	from	ADP
fnp-5883	3	27	a	a	DET
fnp-5883	3	28	liquid	liquid	NOUN
fnp-5883	3	29	to	to	ADP
fnp-5883	3	30	a	a	DET
fnp-5883	3	31	solid	solid	ADJ
fnp-5883	3	32	state	state	NOUN
fnp-5883	3	33	with	with	ADP
fnp-5883	3	34	the	the	DET
fnp-5883	3	35	formation	formation	NOUN
fnp-5883	3	36	of	of	ADP
fnp-5883	3	37	ice	ice	NOUN
fnp-5883	3	38	crystals	crystal	NOUN
fnp-5883	3	39	,	,	PUNCT
fnp-5883	3	40	which	which	PRON
fnp-5883	3	41	can	can	AUX
fnp-5883	3	42	disrupt	disrupt	VERB
fnp-5883	3	43	cellular	cellular	ADJ
fnp-5883	3	44	morphology	morphology	NOUN
fnp-5883	3	45	.	.	PUNCT
fnp-5883	4	1	there	there	PRON
fnp-5883	4	2	is	be	VERB
fnp-5883	4	3	a	a	DET
fnp-5883	4	4	critical	critical	ADJ
fnp-5883	4	5	need	need	NOUN
fnp-5883	4	6	to	to	PART
fnp-5883	4	7	characterize	characterize	VERB
fnp-5883	4	8	the	the	DET
fnp-5883	4	9	effects	effect	NOUN
fnp-5883	4	10	of	of	ADP
fnp-5883	4	11	cryopreservation	cryopreservation	NOUN
fnp-5883	4	12	on	on	ADP
fnp-5883	4	13	brain	brain	NOUN
fnp-5883	4	14	cell	cell	NOUN
fnp-5883	4	15	structure	structure	NOUN
fnp-5883	4	16	at	at	ADP
fnp-5883	4	17	the	the	DET
fnp-5883	4	18	microscopic	microscopic	ADJ
fnp-5883	4	19	level	level	NOUN
fnp-5883	4	20	.	.	PUNCT
fnp-5883	5	1	in	in	ADP
fnp-5883	5	2	this	this	DET
fnp-5883	5	3	review	review	NOUN
fnp-5883	5	4	,	,	PUNCT
fnp-5883	5	5	we	we	PRON
fnp-5883	5	6	conducted	conduct	VERB
fnp-5883	5	7	a	a	DET
fnp-5883	5	8	comprehensive	comprehensive	ADJ
fnp-5883	5	9	literature	literature	NOUN
fnp-5883	5	10	search	search	NOUN
fnp-5883	5	11	,	,	PUNCT
fnp-5883	5	12	identifying	identify	VERB
fnp-5883	5	13	97	97	NUM
fnp-5883	5	14	studies	study	NOUN
fnp-5883	5	15	that	that	PRON
fnp-5883	5	16	yielded	yield	VERB
fnp-5883	5	17	146	146	NUM
fnp-5883	5	18	distinct	distinct	ADJ
fnp-5883	5	19	observations	observation	NOUN
fnp-5883	5	20	of	of	ADP
fnp-5883	5	21	the	the	DET
fnp-5883	5	22	effects	effect	NOUN
fnp-5883	5	23	of	of	ADP
fnp-5883	5	24	cryopreservation	cryopreservation	NOUN
fnp-5883	5	25	on	on	ADP
fnp-5883	5	26	neurohistology	neurohistology	NOUN
fnp-5883	5	27	.	.	PUNCT
fnp-5883	6	1	we	we	PRON
fnp-5883	6	2	classified	classify	VERB
fnp-5883	6	3	the	the	DET
fnp-5883	6	4	reviewed	review	VERB
fnp-5883	6	5	studies	study	NOUN
fnp-5883	6	6	into	into	ADP
fnp-5883	6	7	three	three	NUM
fnp-5883	6	8	main	main	ADJ
fnp-5883	6	9	categories	category	NOUN
fnp-5883	6	10	:	:	PUNCT
fnp-5883	6	11	cryofixation	cryofixation	NOUN
fnp-5883	6	12	,	,	PUNCT
fnp-5883	6	13	freezing	freezing	NOUN
fnp-5883	6	14	,	,	PUNCT
fnp-5883	6	15	and	and	CCONJ
fnp-5883	6	16	cryopreservation	cryopreservation	NOUN
fnp-5883	6	17	with	with	ADP
fnp-5883	6	18	cryoprotectants	cryoprotectant	NOUN
fnp-5883	6	19	.	.	PUNCT
fnp-5883	7	1	cryofixation	cryofixation	NOUN
fnp-5883	7	2	techniques	technique	NOUN
fnp-5883	7	3	enable	enable	VERB
fnp-5883	7	4	vitrification	vitrification	NOUN
fnp-5883	7	5	and	and	CCONJ
fnp-5883	7	6	excellent	excellent	ADJ
fnp-5883	7	7	ultrastructural	ultrastructural	ADJ
fnp-5883	7	8	preservation	preservation	NOUN
fnp-5883	7	9	of	of	ADP
fnp-5883	7	10	thin	thin	ADJ
fnp-5883	7	11	tissue	tissue	NOUN
fnp-5883	7	12	samples	sample	NOUN
fnp-5883	7	13	but	but	CCONJ
fnp-5883	7	14	are	be	AUX
fnp-5883	7	15	limited	limit	VERB
fnp-5883	7	16	in	in	ADP
fnp-5883	7	17	terms	term	NOUN
fnp-5883	7	18	of	of	ADP
fnp-5883	7	19	the	the	DET
fnp-5883	7	20	depth	depth	NOUN
fnp-5883	7	21	of	of	ADP
fnp-5883	7	22	tissue	tissue	NOUN
fnp-5883	7	23	that	that	PRON
fnp-5883	7	24	can	can	AUX
fnp-5883	7	25	be	be	AUX
fnp-5883	7	26	preserved	preserve	VERB
fnp-5883	7	27	without	without	ADP
fnp-5883	7	28	ice	ice	NOUN
fnp-5883	7	29	artifacts	artifact	NOUN
fnp-5883	7	30	.	.	PUNCT
fnp-5883	8	1	freezing	freeze	VERB
fnp-5883	8	2	methods	method	NOUN
fnp-5883	8	3	,	,	PUNCT
fnp-5883	8	4	particularly	particularly	ADV
fnp-5883	8	5	when	when	SCONJ
fnp-5883	8	6	applied	apply	VERB
fnp-5883	8	7	to	to	ADP
fnp-5883	8	8	brain	brain	NOUN
fnp-5883	8	9	slices	slice	NOUN
fnp-5883	8	10	,	,	PUNCT
fnp-5883	8	11	can	can	AUX
fnp-5883	8	12	achieve	achieve	VERB
fnp-5883	8	13	rapid	rapid	ADJ
fnp-5883	8	14	cooling	cool	VERB
fnp-5883	8	15	rates	rate	NOUN
fnp-5883	8	16	that	that	PRON
fnp-5883	8	17	result	result	VERB
fnp-5883	8	18	in	in	ADP
fnp-5883	8	19	minimal	minimal	ADJ
fnp-5883	8	20	ice	ice	NOUN
fnp-5883	8	21	artifacts	artifact	NOUN
fnp-5883	8	22	detectable	detectable	ADJ
fnp-5883	8	23	by	by	ADP
fnp-5883	8	24	light	light	ADJ
fnp-5883	8	25	microscopy	microscopy	NOUN
fnp-5883	8	26	.	.	PUNCT
fnp-5883	9	1	cryoprotectant	cryoprotectant	NOUN
fnp-5883	9	2	-	-	PUNCT
fnp-5883	9	3	based	base	VERB
fnp-5883	9	4	approaches	approach	NOUN
fnp-5883	9	5	have	have	VERB
fnp-5883	9	6	the	the	DET
fnp-5883	9	7	potential	potential	NOUN
fnp-5883	9	8	to	to	PART
fnp-5883	9	9	reduce	reduce	VERB
fnp-5883	9	10	ice	ice	NOUN
fnp-5883	9	11	damage	damage	NOUN
fnp-5883	9	12	and	and	CCONJ
fnp-5883	9	13	achieve	achieve	VERB
fnp-5883	9	14	vitrification	vitrification	NOUN
fnp-5883	9	15	.	.	PUNCT
fnp-5883	10	1	for	for	ADP
fnp-5883	10	2	thin	thin	ADJ
fnp-5883	10	3	tissue	tissue	NOUN
fnp-5883	10	4	samples	sample	NOUN
fnp-5883	10	5	,	,	PUNCT
fnp-5883	10	6	immersion	immersion	NOUN
fnp-5883	10	7	in	in	ADP
fnp-5883	10	8	cryoprotectants	cryoprotectant	NOUN
fnp-5883	10	9	has	have	AUX
fnp-5883	10	10	been	be	AUX
fnp-5883	10	11	found	find	VERB
fnp-5883	10	12	to	to	PART
fnp-5883	10	13	be	be	AUX
fnp-5883	10	14	effective	effective	ADJ
fnp-5883	10	15	for	for	ADP
fnp-5883	10	16	structural	structural	ADJ
fnp-5883	10	17	preservation	preservation	NOUN
fnp-5883	10	18	.	.	PUNCT
fnp-5883	11	1	however	however	ADV
fnp-5883	11	2	,	,	PUNCT
fnp-5883	11	3	for	for	ADP
fnp-5883	11	4	larger	large	ADJ
fnp-5883	11	5	samples	sample	NOUN
fnp-5883	11	6	or	or	CCONJ
fnp-5883	11	7	the	the	DET
fnp-5883	11	8	entire	entire	ADJ
fnp-5883	11	9	brain	brain	NOUN
fnp-5883	11	10	,	,	PUNCT
fnp-5883	11	11	perfusion	perfusion	NOUN
fnp-5883	11	12	of	of	ADP
fnp-5883	11	13	cryoprotectants	cryoprotectant	NOUN
fnp-5883	11	14	is	be	AUX
fnp-5883	11	15	necessary	necessary	ADJ
fnp-5883	11	16	to	to	PART
fnp-5883	11	17	perform	perform	VERB
fnp-5883	11	18	rapid	rapid	ADJ
fnp-5883	11	19	distribution	distribution	NOUN
fnp-5883	11	20	,	,	PUNCT
fnp-5883	11	21	and	and	CCONJ
fnp-5883	11	22	this	this	PRON
fnp-5883	11	23	has	have	VERB
fnp-5883	11	24	a	a	DET
fnp-5883	11	25	more	more	ADV
fnp-5883	11	26	limited	limited	ADJ
fnp-5883	11	27	evidence	evidence	NOUN
fnp-5883	11	28	base	base	NOUN
fnp-5883	11	29	.	.	PUNCT
fnp-5883	12	1	in	in	ADP
fnp-5883	12	2	conclusion	conclusion	NOUN
fnp-5883	12	3	,	,	PUNCT
fnp-5883	12	4	while	while	SCONJ
fnp-5883	12	5	current	current	ADJ
fnp-5883	12	6	cryopreservation	cryopreservation	NOUN
fnp-5883	12	7	methods	method	NOUN
fnp-5883	12	8	can	can	AUX
fnp-5883	12	9	provide	provide	VERB
fnp-5883	12	10	sufficient	sufficient	ADJ
fnp-5883	12	11	quality	quality	NOUN
fnp-5883	12	12	for	for	ADP
fnp-5883	12	13	some	some	DET
fnp-5883	12	14	downstream	downstream	ADJ
fnp-5883	12	15	applications	application	NOUN
fnp-5883	12	16	,	,	PUNCT
fnp-5883	12	17	there	there	PRON
fnp-5883	12	18	is	be	VERB
fnp-5883	12	19	a	a	DET
fnp-5883	12	20	need	need	NOUN
fnp-5883	12	21	for	for	ADP
fnp-5883	12	22	improved	improved	ADJ
fnp-5883	12	23	techniques	technique	NOUN
fnp-5883	12	24	that	that	PRON
fnp-5883	12	25	enable	enable	VERB
fnp-5883	12	26	the	the	DET
fnp-5883	12	27	cryopreservation	cryopreservation	NOUN
fnp-5883	12	28	of	of	ADP
fnp-5883	12	29	larger	large	ADJ
fnp-5883	12	30	brain	brain	NOUN
fnp-5883	12	31	tissue	tissue	NOUN
fnp-5883	12	32	samples	sample	NOUN
fnp-5883	12	33	while	while	SCONJ
fnp-5883	12	34	maintaining	maintain	VERB
fnp-5883	12	35	excellent	excellent	ADJ
fnp-5883	12	36	structural	structural	ADJ
fnp-5883	12	37	preservation	preservation	NOUN
fnp-5883	12	38	.	.	PUNCT
fnp-5883	13	1	table	table	NOUN
fnp-5883	13	2	of	of	ADP
fnp-5883	13	3	contents	content	NOUN
fnp-5883	13	4	introduction	introduction	NOUN
fnp-5883	13	5	methods	method	NOUN
fnp-5883	13	6	realist	realist	ADJ
fnp-5883	13	7	synthesis	synthesis	NOUN
fnp-5883	13	8	eligibility	eligibility	NOUN
fnp-5883	13	9	criteria	criterion	NOUN
fnp-5883	13	10	search	search	NOUN
fnp-5883	13	11	methods	method	NOUN
fnp-5883	13	12	and	and	CCONJ
fnp-5883	13	13	study	study	NOUN
fnp-5883	13	14	selection	selection	NOUN
fnp-5883	13	15	data	datum	NOUN
fnp-5883	13	16	extraction	extraction	NOUN
fnp-5883	13	17	qualitative	qualitative	VERB
fnp-5883	13	18	data	datum	NOUN
fnp-5883	13	19	analysis	analysis	NOUN
fnp-5883	13	20	example	example	NOUN
fnp-5883	13	21	frozen	frozen	ADJ
fnp-5883	13	22	brain	brain	NOUN
fnp-5883	13	23	tissue	tissue	NOUN
fnp-5883	13	24	data	datum	NOUN
fnp-5883	13	25	characteristics	characteristic	NOUN
fnp-5883	13	26	of	of	ADP
fnp-5883	13	27	included	include	VERB
fnp-5883	13	28	studies	study	NOUN
fnp-5883	13	29	mechanisms	mechanism	NOUN
fnp-5883	13	30	of	of	ADP
fnp-5883	13	31	brain	brain	NOUN
fnp-5883	13	32	cryopreservation	cryopreservation	NOUN
fnp-5883	13	33	physical	physical	ADJ
fnp-5883	13	34	aspects	aspect	NOUN
fnp-5883	13	35	of	of	ADP
fnp-5883	13	36	cooling	cool	VERB
fnp-5883	13	37	how	how	SCONJ
fnp-5883	13	38	ice	ice	NOUN
fnp-5883	13	39	damages	damage	NOUN
fnp-5883	13	40	biological	biological	ADJ
fnp-5883	13	41	tissue	tissue	NOUN
fnp-5883	13	42	how	how	SCONJ
fnp-5883	13	43	ice	ice	NOUN
fnp-5883	13	44	damage	damage	NOUN
fnp-5883	13	45	can	can	AUX
fnp-5883	13	46	be	be	AUX
fnp-5883	13	47	prevented	prevent	VERB
fnp-5883	13	48	ice	ice	NOUN
fnp-5883	13	49	formation	formation	NOUN
fnp-5883	13	50	during	during	ADP
fnp-5883	13	51	freezing	freeze	VERB
fnp-5883	13	52	procedures	procedure	NOUN
fnp-5883	13	53	eutectic	eutectic	ADJ
fnp-5883	13	54	crystallization	crystallization	NOUN
fnp-5883	13	55	cellular	cellular	ADJ
fnp-5883	13	56	decomposition	decomposition	NOUN
fnp-5883	13	57	fractures	fracture	NOUN
fnp-5883	13	58	summary	summary	NOUN
fnp-5883	13	59	cryofixation	cryofixation	NOUN
fnp-5883	13	60	preservation	preservation	NOUN
fnp-5883	13	61	process	process	NOUN
fnp-5883	13	62	effects	effect	NOUN
fnp-5883	13	63	on	on	ADP
fnp-5883	13	64	tissue	tissue	NOUN
fnp-5883	13	65	morphology	morphology	NOUN
fnp-5883	13	66	ice	ice	NOUN
fnp-5883	13	67	damage	damage	NOUN
fnp-5883	13	68	damage	damage	NOUN
fnp-5883	13	69	by	by	ADP
fnp-5883	13	70	tissue	tissue	NOUN
fnp-5883	13	71	cutting	cutting	NOUN
fnp-5883	13	72	and	and	CCONJ
fnp-5883	13	73	handling	handle	VERB
fnp-5883	13	74	summary	summary	NOUN
fnp-5883	13	75	freezing	freezing	NOUN
fnp-5883	13	76	without	without	ADP
fnp-5883	13	77	cryoprotectants	cryoprotectant	NOUN
fnp-5883	13	78	preservation	preservation	NOUN
fnp-5883	13	79	process	process	NOUN
fnp-5883	13	80	effects	effect	NOUN
fnp-5883	13	81	on	on	ADP
fnp-5883	13	82	tissue	tissue	NOUN
fnp-5883	13	83	morphology	morphology	NOUN
fnp-5883	13	84	comparison	comparison	NOUN
fnp-5883	13	85	of	of	ADP
fnp-5883	13	86	methods	method	NOUN
fnp-5883	13	87	damage	damage	NOUN
fnp-5883	13	88	during	during	ADP
fnp-5883	13	89	tissue	tissue	NOUN
fnp-5883	13	90	rewarming	rewarme	VERB
fnp-5883	13	91	tissue	tissue	NOUN
fnp-5883	13	92	freezing	freezing	NOUN
fnp-5883	13	93	in	in	ADP
fnp-5883	13	94	brain	brain	NOUN
fnp-5883	13	95	banking	banking	NOUN
fnp-5883	13	96	summary	summary	NOUN
fnp-5883	13	97	cryoprotectant	cryoprotectant	NOUN
fnp-5883	13	98	-	-	PUNCT
fnp-5883	13	99	based	base	VERB
fnp-5883	13	100	cryopreservation	cryopreservation	NOUN
fnp-5883	13	101	preservation	preservation	NOUN
fnp-5883	13	102	process	process	NOUN
fnp-5883	13	103	cryoprotectant	cryoprotectant	NOUN
fnp-5883	13	104	immersion	immersion	NOUN
fnp-5883	13	105	cryoprotectant	cryoprotectant	NOUN
fnp-5883	13	106	perfusion	perfusion	NOUN
fnp-5883	13	107	summary	summary	NOUN
fnp-5883	13	108	storage	storage	NOUN
fnp-5883	13	109	temperature	temperature	NOUN
fnp-5883	13	110	and	and	CCONJ
fnp-5883	13	111	length	length	NOUN
fnp-5883	13	112	relationship	relationship	NOUN
fnp-5883	13	113	between	between	ADP
fnp-5883	13	114	ice	ice	NOUN
fnp-5883	13	115	crystal	crystal	NOUN
fnp-5883	13	116	formation	formation	NOUN
fnp-5883	13	117	and	and	CCONJ
fnp-5883	13	118	postmortem	postmortem	NOUN
fnp-5883	13	119	decomposition	decomposition	NOUN
fnp-5883	13	120	effects	effect	NOUN
fnp-5883	13	121	of	of	ADP
fnp-5883	13	122	cryopreservation	cryopreservation	NOUN
fnp-5883	13	123	on	on	ADP
fnp-5883	13	124	biomolecular	biomolecular	ADJ
fnp-5883	13	125	preservation	preservation	NOUN
fnp-5883	13	126	comparison	comparison	NOUN
fnp-5883	13	127	to	to	ADP
fnp-5883	13	128	other	other	ADJ
fnp-5883	13	129	tissues	tissue	NOUN
fnp-5883	13	130	comparison	comparison	NOUN
fnp-5883	13	131	to	to	ADP
fnp-5883	13	132	previous	previous	ADJ
fnp-5883	13	133	reviews	review	NOUN
fnp-5883	13	134	limitations	limitation	NOUN
fnp-5883	13	135	of	of	ADP
fnp-5883	13	136	this	this	DET
fnp-5883	13	137	review	review	NOUN
fnp-5883	13	138	summary	summary	NOUN
fnp-5883	13	139	of	of	ADP
fnp-5883	13	140	cryopreservation	cryopreservation	NOUN
fnp-5883	13	141	methods	method	NOUN
fnp-5883	13	142	discussed	discuss	VERB
fnp-5883	13	143	implications	implication	NOUN
fnp-5883	13	144	for	for	ADP
fnp-5883	13	145	brain	brain	NOUN
fnp-5883	13	146	banking	banking	NOUN
fnp-5883	13	147	areas	area	NOUN
fnp-5883	13	148	for	for	ADP
fnp-5883	13	149	further	further	ADJ
fnp-5883	13	150	research	research	NOUN
fnp-5883	13	151	conclusions	conclusion	NOUN
fnp-5883	13	152	abbreviations	abbreviation	NOUN
fnp-5883	13	153	acknowledgements	acknowledgement	VERB
fnp-5883	13	154	data	datum	NOUN
fnp-5883	13	155	availability	availability	NOUN
fnp-5883	13	156	author	author	NOUN
fnp-5883	13	157	contributions	contribution	NOUN
fnp-5883	13	158	conflicts	conflict	NOUN
fnp-5883	13	159	of	of	ADP
fnp-5883	13	160	interest	interest	NOUN
fnp-5883	13	161	statement	statement	NOUN
fnp-5883	13	162	funding	fund	VERB
fnp-5883	13	163	statement	statement	NOUN
fnp-5883	13	164	supplementary	supplementary	ADJ
fnp-5883	13	165	files	file	NOUN
fnp-5883	13	166	references	reference	NOUN
fnp-5883	13	167	introduction	introduction	NOUN
fnp-5883	13	168	cryopreservation	cryopreservation	NOUN
fnp-5883	13	169	is	be	AUX
fnp-5883	13	170	a	a	DET
fnp-5883	13	171	key	key	ADJ
fnp-5883	13	172	technique	technique	NOUN
fnp-5883	13	173	in	in	ADP
fnp-5883	13	174	contemporary	contemporary	ADJ
fnp-5883	13	175	brain	brain	NOUN
fnp-5883	13	176	banking	banking	NOUN
fnp-5883	13	177	(	(	PUNCT
fnp-5883	13	178	mckee	mckee	PROPN
fnp-5883	13	179	,	,	PUNCT
fnp-5883	13	180	1999	1999	NUM
fnp-5883	13	181	;	;	PUNCT
fnp-5883	13	182	vonsattel	vonsattel	NOUN
fnp-5883	13	183	et	et	PROPN
fnp-5883	13	184	al	al	PROPN
fnp-5883	13	185	.	.	PROPN
fnp-5883	13	186	,	,	PUNCT
fnp-5883	13	187	2008	2008	NUM
fnp-5883	13	188	;	;	PUNCT
fnp-5883	14	1	ramirez	ramirez	PROPN
fnp-5883	14	2	et	et	PROPN
fnp-5883	14	3	al	al	PROPN
fnp-5883	14	4	.	.	PROPN
fnp-5883	14	5	,	,	PUNCT
fnp-5883	14	6	2018	2018	NUM
fnp-5883	14	7	)	)	PUNCT
fnp-5883	14	8	.	.	PUNCT
fnp-5883	15	1	here	here	ADV
fnp-5883	15	2	,	,	PUNCT
fnp-5883	15	3	we	we	PRON
fnp-5883	15	4	define	define	VERB
fnp-5883	15	5	cryopreservation	cryopreservation	NOUN
fnp-5883	15	6	as	as	ADP
fnp-5883	15	7	the	the	DET
fnp-5883	15	8	preservation	preservation	NOUN
fnp-5883	15	9	of	of	ADP
fnp-5883	15	10	tissue	tissue	NOUN
fnp-5883	15	11	at	at	ADP
fnp-5883	15	12	temperatures	temperature	NOUN
fnp-5883	15	13	below	below	ADP
fnp-5883	15	14	0	0	NUM
fnp-5883	15	15	 	 	SPACE
fnp-5883	15	16	°	°	NOUN
fnp-5883	15	17	c	c	NOUN
fnp-5883	15	18	.	.	PUNCT
fnp-5883	16	1	this	this	PRON
fnp-5883	16	2	can	can	AUX
fnp-5883	16	3	allow	allow	VERB
fnp-5883	16	4	for	for	ADP
fnp-5883	16	5	the	the	DET
fnp-5883	16	6	long	long	ADJ
fnp-5883	16	7	-	-	PUNCT
fnp-5883	16	8	term	term	NOUN
fnp-5883	16	9	storage	storage	NOUN
fnp-5883	16	10	of	of	ADP
fnp-5883	16	11	preserved	preserve	VERB
fnp-5883	16	12	brain	brain	NOUN
fnp-5883	16	13	tissue	tissue	NOUN
fnp-5883	16	14	for	for	ADP
fnp-5883	16	15	subsequent	subsequent	ADJ
fnp-5883	16	16	research	research	NOUN
fnp-5883	16	17	,	,	PUNCT
fnp-5883	16	18	where	where	SCONJ
fnp-5883	16	19	it	it	PRON
fnp-5883	16	20	is	be	AUX
fnp-5883	16	21	frequently	frequently	ADV
fnp-5883	16	22	used	use	VERB
fnp-5883	16	23	for	for	ADP
fnp-5883	16	24	biomolecular	biomolecular	ADJ
fnp-5883	16	25	profiling	profiling	NOUN
fnp-5883	16	26	studies	study	NOUN
fnp-5883	16	27	such	such	ADJ
fnp-5883	16	28	as	as	ADP
fnp-5883	16	29	nucleic	nucleic	ADJ
fnp-5883	16	30	acid	acid	NOUN
fnp-5883	16	31	sequencing	sequencing	NOUN
fnp-5883	16	32	or	or	CCONJ
fnp-5883	16	33	metabolomics	metabolomic	NOUN
fnp-5883	16	34	.	.	PUNCT
fnp-5883	17	1	cryopreservation	cryopreservation	NOUN
fnp-5883	17	2	preserves	preserve	VERB
fnp-5883	17	3	tissue	tissue	NOUN
fnp-5883	17	4	by	by	ADP
fnp-5883	17	5	decreasing	decrease	VERB
fnp-5883	17	6	the	the	DET
fnp-5883	17	7	rate	rate	NOUN
fnp-5883	17	8	of	of	ADP
fnp-5883	17	9	molecular	molecular	ADJ
fnp-5883	17	10	motion	motion	NOUN
fnp-5883	17	11	across	across	ADP
fnp-5883	17	12	an	an	DET
fnp-5883	17	13	entire	entire	ADJ
fnp-5883	17	14	biospecimen	biospeciman	NOUN
fnp-5883	17	15	(	(	PUNCT
fnp-5883	17	16	wowk	wowk	PROPN
fnp-5883	17	17	,	,	PUNCT
fnp-5883	17	18	2010	2010	NUM
fnp-5883	17	19	;	;	PUNCT
fnp-5883	17	20	weng	weng	PROPN
fnp-5883	17	21	et	et	PROPN
fnp-5883	17	22	al	al	PROPN
fnp-5883	17	23	.	.	PROPN
fnp-5883	17	24	,	,	PUNCT
fnp-5883	17	25	2019	2019	NUM
fnp-5883	17	26	)	)	PUNCT
fnp-5883	17	27	.	.	PUNCT
fnp-5883	18	1	however	however	ADV
fnp-5883	18	2	,	,	PUNCT
fnp-5883	18	3	cryopreservation	cryopreservation	NOUN
fnp-5883	18	4	can	can	AUX
fnp-5883	18	5	cause	cause	VERB
fnp-5883	18	6	several	several	ADJ
fnp-5883	18	7	types	type	NOUN
fnp-5883	18	8	of	of	ADP
fnp-5883	18	9	structural	structural	ADJ
fnp-5883	18	10	artifacts	artifact	NOUN
fnp-5883	18	11	,	,	PUNCT
fnp-5883	18	12	including	include	VERB
fnp-5883	18	13	ice	ice	NOUN
fnp-5883	18	14	formation	formation	NOUN
fnp-5883	18	15	,	,	PUNCT
fnp-5883	18	16	dehydration	dehydration	NOUN
fnp-5883	18	17	,	,	PUNCT
fnp-5883	18	18	displacement	displacement	NOUN
fnp-5883	18	19	of	of	ADP
fnp-5883	18	20	tissue	tissue	NOUN
fnp-5883	18	21	structures	structure	NOUN
fnp-5883	18	22	,	,	PUNCT
fnp-5883	18	23	and	and	CCONJ
fnp-5883	18	24	local	local	ADJ
fnp-5883	18	25	aggregation	aggregation	NOUN
fnp-5883	18	26	of	of	ADP
fnp-5883	18	27	biomolecules	biomolecule	NOUN
fnp-5883	18	28	(	(	PUNCT
fnp-5883	18	29	rosene	rosene	NOUN
fnp-5883	18	30	and	and	CCONJ
fnp-5883	18	31	rhodes	rhode	NOUN
fnp-5883	18	32	,	,	PUNCT
fnp-5883	18	33	1990	1990	NUM
fnp-5883	18	34	;	;	PUNCT
fnp-5883	18	35	jain	jain	PROPN
fnp-5883	18	36	et	et	PROPN
fnp-5883	18	37	al	al	PROPN
fnp-5883	18	38	.	.	PROPN
fnp-5883	18	39	,	,	PUNCT
fnp-5883	18	40	2021	2021	NUM
fnp-5883	18	41	)	)	PUNCT
fnp-5883	18	42	.	.	PUNCT
fnp-5883	19	1	these	these	DET
fnp-5883	19	2	artifacts	artifact	NOUN
fnp-5883	19	3	can	can	AUX
fnp-5883	19	4	be	be	AUX
fnp-5883	19	5	ameliorated	ameliorate	VERB
fnp-5883	19	6	by	by	ADP
fnp-5883	19	7	vitrification	vitrification	NOUN
fnp-5883	19	8	,	,	PUNCT
fnp-5883	19	9	wherein	wherein	ADJ
fnp-5883	19	10	tissue	tissue	NOUN
fnp-5883	19	11	is	be	AUX
fnp-5883	19	12	cooled	cool	VERB
fnp-5883	19	13	to	to	PART
fnp-5883	19	14	form	form	VERB
fnp-5883	19	15	a	a	DET
fnp-5883	19	16	glass	glass	NOUN
fnp-5883	19	17	-	-	PUNCT
fnp-5883	19	18	like	like	ADJ
fnp-5883	19	19	amorphous	amorphous	ADJ
fnp-5883	19	20	solid	solid	ADJ
fnp-5883	19	21	without	without	ADP
fnp-5883	19	22	ice	ice	NOUN
fnp-5883	19	23	crystal	crystal	NOUN
fnp-5883	19	24	formation	formation	NOUN
fnp-5883	19	25	,	,	PUNCT
fnp-5883	19	26	although	although	SCONJ
fnp-5883	19	27	this	this	PRON
fnp-5883	19	28	can	can	AUX
fnp-5883	19	29	be	be	AUX
fnp-5883	19	30	difficult	difficult	ADJ
fnp-5883	19	31	to	to	PART
fnp-5883	19	32	achieve	achieve	VERB
fnp-5883	19	33	in	in	ADP
fnp-5883	19	34	the	the	DET
fnp-5883	19	35	large	large	ADJ
fnp-5883	19	36	tissue	tissue	NOUN
fnp-5883	19	37	volumes	volume	NOUN
fnp-5883	19	38	typical	typical	ADJ
fnp-5883	19	39	of	of	ADP
fnp-5883	19	40	brain	brain	NOUN
fnp-5883	19	41	banking	banking	NOUN
fnp-5883	19	42	(	(	PUNCT
fnp-5883	19	43	wowk	wowk	NOUN
fnp-5883	19	44	,	,	PUNCT
fnp-5883	19	45	2010	2010	NUM
fnp-5883	19	46	)	)	PUNCT
fnp-5883	19	47	.	.	PUNCT
fnp-5883	20	1	advances	advance	NOUN
fnp-5883	20	2	in	in	ADP
fnp-5883	20	3	refrigeration	refrigeration	NOUN
fnp-5883	20	4	,	,	PUNCT
fnp-5883	20	5	cryogen	cryogen	NOUN
fnp-5883	20	6	production	production	NOUN
fnp-5883	20	7	,	,	PUNCT
fnp-5883	20	8	cryobiology	cryobiology	NOUN
fnp-5883	20	9	,	,	PUNCT
fnp-5883	20	10	and	and	CCONJ
fnp-5883	20	11	their	their	PRON
fnp-5883	20	12	application	application	NOUN
fnp-5883	20	13	in	in	ADP
fnp-5883	20	14	brain	brain	NOUN
fnp-5883	20	15	banking	banking	NOUN
fnp-5883	20	16	over	over	ADP
fnp-5883	20	17	the	the	DET
fnp-5883	20	18	last	last	ADJ
fnp-5883	20	19	several	several	ADJ
fnp-5883	20	20	decades	decade	NOUN
fnp-5883	20	21	have	have	AUX
fnp-5883	20	22	allowed	allow	VERB
fnp-5883	20	23	a	a	DET
fnp-5883	20	24	set	set	NOUN
fnp-5883	20	25	of	of	ADP
fnp-5883	20	26	best	good	ADJ
fnp-5883	20	27	practices	practice	NOUN
fnp-5883	20	28	to	to	PART
fnp-5883	20	29	emerge	emerge	VERB
fnp-5883	20	30	for	for	ADP
fnp-5883	20	31	how	how	SCONJ
fnp-5883	20	32	brain	brain	NOUN
fnp-5883	20	33	tissue	tissue	NOUN
fnp-5883	20	34	should	should	AUX
fnp-5883	20	35	be	be	AUX
fnp-5883	20	36	cryopreserved	cryopreserve	VERB
fnp-5883	20	37	.	.	PUNCT
fnp-5883	21	1	for	for	ADP
fnp-5883	21	2	example	example	NOUN
fnp-5883	21	3	,	,	PUNCT
fnp-5883	21	4	many	many	ADJ
fnp-5883	21	5	brain	brain	NOUN
fnp-5883	21	6	banks	bank	NOUN
fnp-5883	21	7	rapidly	rapidly	ADV
fnp-5883	21	8	cool	cool	ADJ
fnp-5883	21	9	slabs	slab	NOUN
fnp-5883	21	10	of	of	ADP
fnp-5883	21	11	fresh	fresh	ADJ
fnp-5883	21	12	brain	brain	NOUN
fnp-5883	21	13	tissue	tissue	NOUN
fnp-5883	21	14	between	between	ADP
fnp-5883	21	15	cooled	cool	VERB
fnp-5883	21	16	metal	metal	NOUN
fnp-5883	21	17	plates	plate	NOUN
fnp-5883	21	18	(	(	PUNCT
fnp-5883	21	19	jones	jones	PROPN
fnp-5883	21	20	et	et	PROPN
fnp-5883	21	21	al	al	PROPN
fnp-5883	21	22	.	.	PROPN
fnp-5883	21	23	,	,	PUNCT
fnp-5883	21	24	1992	1992	NUM
fnp-5883	21	25	;	;	PUNCT
fnp-5883	21	26	vonsattel	vonsattel	NOUN
fnp-5883	21	27	et	et	PROPN
fnp-5883	21	28	al	al	PROPN
fnp-5883	21	29	.	.	PROPN
fnp-5883	21	30	,	,	PUNCT
fnp-5883	21	31	2008	2008	NUM
fnp-5883	21	32	)	)	PUNCT
fnp-5883	21	33	.	.	PUNCT
fnp-5883	22	1	despite	despite	SCONJ
fnp-5883	22	2	these	these	DET
fnp-5883	22	3	advances	advance	NOUN
fnp-5883	22	4	,	,	PUNCT
fnp-5883	22	5	better	well	ADJ
fnp-5883	22	6	cryopreservation	cryopreservation	NOUN
fnp-5883	22	7	solutions	solution	NOUN
fnp-5883	22	8	are	be	AUX
fnp-5883	22	9	clearly	clearly	ADV
fnp-5883	22	10	needed	need	VERB
fnp-5883	22	11	for	for	ADP
fnp-5883	22	12	many	many	ADJ
fnp-5883	22	13	potential	potential	ADJ
fnp-5883	22	14	research	research	NOUN
fnp-5883	22	15	applications	application	NOUN
fnp-5883	22	16	.	.	PUNCT
fnp-5883	23	1	as	as	ADP
fnp-5883	23	2	a	a	DET
fnp-5883	23	3	result	result	NOUN
fnp-5883	23	4	,	,	PUNCT
fnp-5883	23	5	there	there	PRON
fnp-5883	23	6	is	be	VERB
fnp-5883	23	7	a	a	DET
fnp-5883	23	8	critical	critical	ADJ
fnp-5883	23	9	need	need	NOUN
fnp-5883	23	10	to	to	PART
fnp-5883	23	11	examine	examine	VERB
fnp-5883	23	12	the	the	DET
fnp-5883	23	13	effects	effect	NOUN
fnp-5883	23	14	of	of	ADP
fnp-5883	23	15	cryopreservation	cryopreservation	NOUN
fnp-5883	23	16	on	on	ADP
fnp-5883	23	17	brain	brain	NOUN
fnp-5883	23	18	cell	cell	NOUN
fnp-5883	23	19	structure	structure	NOUN
fnp-5883	23	20	,	,	PUNCT
fnp-5883	23	21	to	to	PART
fnp-5883	23	22	better	well	ADV
fnp-5883	23	23	understand	understand	VERB
fnp-5883	23	24	what	what	PRON
fnp-5883	23	25	the	the	DET
fnp-5883	23	26	state	state	NOUN
fnp-5883	23	27	-	-	PUNCT
fnp-5883	23	28	of	of	ADP
fnp-5883	23	29	-	-	PUNCT
fnp-5883	23	30	the	the	DET
fnp-5883	23	31	-	-	PUNCT
fnp-5883	23	32	art	art	NOUN
fnp-5883	23	33	methods	method	NOUN
fnp-5883	23	34	are	be	AUX
fnp-5883	23	35	and	and	CCONJ
fnp-5883	23	36	how	how	SCONJ
fnp-5883	23	37	they	they	PRON
fnp-5883	23	38	could	could	AUX
fnp-5883	23	39	potentially	potentially	ADV
fnp-5883	23	40	be	be	AUX
fnp-5883	23	41	improved	improve	VERB
fnp-5883	23	42	upon	upon	SCONJ
fnp-5883	23	43	.	.	PUNCT
fnp-5883	24	1	our	our	PRON
fnp-5883	24	2	review	review	NOUN
fnp-5883	24	3	focuses	focus	VERB
fnp-5883	24	4	on	on	ADP
fnp-5883	24	5	unfixed	unfixed	ADJ
fnp-5883	24	6	tissue	tissue	NOUN
fnp-5883	24	7	.	.	PUNCT
fnp-5883	25	1	however	however	ADV
fnp-5883	25	2	,	,	PUNCT
fnp-5883	25	3	we	we	PRON
fnp-5883	25	4	note	note	VERB
fnp-5883	25	5	that	that	SCONJ
fnp-5883	25	6	there	there	PRON
fnp-5883	25	7	is	be	VERB
fnp-5883	25	8	an	an	DET
fnp-5883	25	9	extensive	extensive	ADJ
fnp-5883	25	10	literature	literature	NOUN
fnp-5883	25	11	showing	show	VERB
fnp-5883	25	12	that	that	SCONJ
fnp-5883	25	13	brain	brain	NOUN
fnp-5883	25	14	tissue	tissue	NOUN
fnp-5883	25	15	,	,	PUNCT
fnp-5883	25	16	which	which	PRON
fnp-5883	25	17	is	be	AUX
fnp-5883	25	18	pre	pre	ADJ
fnp-5883	25	19	-	-	VERB
fnp-5883	25	20	fixed	fix	VERB
fnp-5883	25	21	with	with	ADP
fnp-5883	25	22	chemicals	chemical	NOUN
fnp-5883	25	23	can	can	AUX
fnp-5883	25	24	be	be	AUX
fnp-5883	25	25	effectively	effectively	ADV
fnp-5883	25	26	cryopreserved	cryopreserve	VERB
fnp-5883	25	27	with	with	ADP
fnp-5883	25	28	the	the	DET
fnp-5883	25	29	use	use	NOUN
fnp-5883	25	30	of	of	ADP
fnp-5883	25	31	cryoprotectants	cryoprotectant	NOUN
fnp-5883	25	32	(	(	PUNCT
fnp-5883	25	33	watson	watson	PROPN
fnp-5883	25	34	et	et	PROPN
fnp-5883	25	35	al	al	PROPN
fnp-5883	25	36	.	.	PROPN
fnp-5883	25	37	,	,	PUNCT
fnp-5883	25	38	1986	1986	NUM
fnp-5883	25	39	;	;	PUNCT
fnp-5883	25	40	meissner	meissner	PROPN
fnp-5883	25	41	and	and	CCONJ
fnp-5883	25	42	schwarz	schwarz	NOUN
fnp-5883	25	43	,	,	PUNCT
fnp-5883	25	44	1990	1990	NUM
fnp-5883	25	45	;	;	PUNCT
fnp-5883	25	46	otubo	otubo	PROPN
fnp-5883	25	47	et	et	PROPN
fnp-5883	25	48	al	al	PROPN
fnp-5883	25	49	.	.	PROPN
fnp-5883	25	50	,	,	PUNCT
fnp-5883	25	51	2021	2021	NUM
fnp-5883	25	52	)	)	PUNCT
fnp-5883	25	53	.	.	PUNCT
fnp-5883	26	1	chemical	chemical	NOUN
fnp-5883	26	2	fixation	fixation	NOUN
fnp-5883	26	3	aids	aid	NOUN
fnp-5883	26	4	in	in	ADP
fnp-5883	26	5	the	the	DET
fnp-5883	26	6	process	process	NOUN
fnp-5883	26	7	of	of	ADP
fnp-5883	26	8	cryoprotection	cryoprotection	NOUN
fnp-5883	26	9	because	because	SCONJ
fnp-5883	26	10	it	it	PRON
fnp-5883	26	11	prevents	prevent	VERB
fnp-5883	26	12	decomposition	decomposition	NOUN
fnp-5883	26	13	during	during	ADP
fnp-5883	26	14	cryoprotectant	cryoprotectant	NOUN
fnp-5883	26	15	diffusion	diffusion	NOUN
fnp-5883	26	16	,	,	PUNCT
fnp-5883	26	17	it	it	PRON
fnp-5883	26	18	mitigates	mitigate	VERB
fnp-5883	26	19	damage	damage	NOUN
fnp-5883	26	20	due	due	ADP
fnp-5883	26	21	to	to	ADP
fnp-5883	26	22	dehydration	dehydration	NOUN
fnp-5883	26	23	,	,	PUNCT
fnp-5883	26	24	and	and	CCONJ
fnp-5883	26	25	it	it	PRON
fnp-5883	26	26	stabilizes	stabilize	VERB
fnp-5883	26	27	biomolecules	biomolecule	NOUN
fnp-5883	26	28	in	in	ADP
fnp-5883	26	29	place	place	NOUN
fnp-5883	26	30	(	(	PUNCT
fnp-5883	26	31	paljärvi	paljärvi	VERB
fnp-5883	26	32	et	et	PROPN
fnp-5883	26	33	al	al	PROPN
fnp-5883	26	34	.	.	PROPN
fnp-5883	26	35	,	,	PUNCT
fnp-5883	26	36	1979	1979	NUM
fnp-5883	26	37	;	;	PUNCT
fnp-5883	26	38	robards	robard	NOUN
fnp-5883	26	39	and	and	CCONJ
fnp-5883	26	40	sleytr	sleytr	NOUN
fnp-5883	26	41	,	,	PUNCT
fnp-5883	26	42	1985	1985	NUM
fnp-5883	26	43	,	,	PUNCT
fnp-5883	26	44	p.	p.	NOUN
fnp-5883	26	45	 	 	SPACE
fnp-5883	26	46	88	88	NUM
fnp-5883	26	47	;	;	PUNCT
fnp-5883	26	48	meissner	meissner	NOUN
fnp-5883	26	49	and	and	CCONJ
fnp-5883	26	50	schwarz	schwarz	NOUN
fnp-5883	26	51	,	,	PUNCT
fnp-5883	26	52	1990	1990	NUM
fnp-5883	26	53	)	)	PUNCT
fnp-5883	26	54	.	.	PUNCT
fnp-5883	27	1	perfusion	perfusion	NOUN
fnp-5883	27	2	with	with	ADP
fnp-5883	27	3	fixatives	fixative	NOUN
fnp-5883	27	4	has	have	AUX
fnp-5883	27	5	also	also	ADV
fnp-5883	27	6	been	be	AUX
fnp-5883	27	7	shown	show	VERB
fnp-5883	27	8	to	to	PART
fnp-5883	27	9	help	help	VERB
fnp-5883	27	10	with	with	ADP
fnp-5883	27	11	subsequent	subsequent	ADJ
fnp-5883	27	12	perfusion	perfusion	NOUN
fnp-5883	27	13	of	of	ADP
fnp-5883	27	14	cryoprotectant	cryoprotectant	NOUN
fnp-5883	27	15	,	,	PUNCT
fnp-5883	27	16	possibly	possibly	ADV
fnp-5883	27	17	because	because	SCONJ
fnp-5883	27	18	fixation	fixation	NOUN
fnp-5883	27	19	helps	help	VERB
fnp-5883	27	20	to	to	PART
fnp-5883	27	21	stabilize	stabilize	VERB
fnp-5883	27	22	blood	blood	NOUN
fnp-5883	27	23	vessels	vessel	NOUN
fnp-5883	27	24	(	(	PUNCT
fnp-5883	27	25	mcintyre	mcintyre	PROPN
fnp-5883	27	26	and	and	CCONJ
fnp-5883	27	27	fahy	fahy	PROPN
fnp-5883	27	28	,	,	PUNCT
fnp-5883	27	29	2015	2015	NUM
fnp-5883	27	30	)	)	PUNCT
fnp-5883	27	31	.	.	PUNCT
fnp-5883	28	1	with	with	ADP
fnp-5883	28	2	the	the	DET
fnp-5883	28	3	use	use	NOUN
fnp-5883	28	4	of	of	ADP
fnp-5883	28	5	sufficient	sufficient	ADJ
fnp-5883	28	6	cryoprotection	cryoprotection	NOUN
fnp-5883	28	7	,	,	PUNCT
fnp-5883	28	8	the	the	DET
fnp-5883	28	9	tissue	tissue	NOUN
fnp-5883	28	10	can	can	AUX
fnp-5883	28	11	be	be	AUX
fnp-5883	28	12	vitrified	vitrify	VERB
fnp-5883	28	13	,	,	PUNCT
fnp-5883	28	14	entirely	entirely	ADV
fnp-5883	28	15	preventing	prevent	VERB
fnp-5883	28	16	ice	ice	NOUN
fnp-5883	28	17	formation	formation	NOUN
fnp-5883	28	18	and	and	CCONJ
fnp-5883	28	19	associated	associate	VERB
fnp-5883	28	20	ice	ice	NOUN
fnp-5883	28	21	artifacts	artifact	NOUN
fnp-5883	28	22	(	(	PUNCT
fnp-5883	28	23	mcintyre	mcintyre	PROPN
fnp-5883	28	24	and	and	CCONJ
fnp-5883	28	25	fahy	fahy	PROPN
fnp-5883	28	26	,	,	PUNCT
fnp-5883	28	27	2015	2015	NUM
fnp-5883	28	28	)	)	PUNCT
fnp-5883	28	29	.	.	PUNCT
fnp-5883	29	1	chemical	chemical	NOUN
fnp-5883	29	2	fixation	fixation	NOUN
fnp-5883	29	3	prevents	prevent	VERB
fnp-5883	29	4	cellular	cellular	ADJ
fnp-5883	29	5	damage	damage	NOUN
fnp-5883	29	6	during	during	ADP
fnp-5883	29	7	cryoprotection	cryoprotection	NOUN
fnp-5883	29	8	,	,	PUNCT
fnp-5883	29	9	aids	aid	NOUN
fnp-5883	29	10	with	with	ADP
fnp-5883	29	11	the	the	DET
fnp-5883	29	12	membrane	membrane	NOUN
fnp-5883	29	13	permeability	permeability	NOUN
fnp-5883	29	14	of	of	ADP
fnp-5883	29	15	cryoprotectants	cryoprotectant	NOUN
fnp-5883	29	16	,	,	PUNCT
fnp-5883	29	17	and	and	CCONJ
fnp-5883	29	18	may	may	AUX
fnp-5883	29	19	help	help	VERB
fnp-5883	29	20	stabilize	stabilize	VERB
fnp-5883	29	21	membrane	membrane	NOUN
fnp-5883	29	22	structure	structure	NOUN
fnp-5883	29	23	by	by	ADP
fnp-5883	29	24	“	"	PUNCT
fnp-5883	29	25	hardening	harden	VERB
fnp-5883	29	26	”	"	PUNCT
fnp-5883	29	27	the	the	DET
fnp-5883	29	28	lipids	lipid	NOUN
fnp-5883	29	29	(	(	PUNCT
fnp-5883	29	30	moor	moor	PROPN
fnp-5883	29	31	et	et	PROPN
fnp-5883	29	32	al	al	PROPN
fnp-5883	29	33	.	.	PROPN
fnp-5883	29	34	,	,	PUNCT
fnp-5883	29	35	1980	1980	NUM
fnp-5883	29	36	)	)	PUNCT
fnp-5883	29	37	.	.	PUNCT
fnp-5883	30	1	there	there	PRON
fnp-5883	30	2	are	be	VERB
fnp-5883	30	3	methods	method	NOUN
fnp-5883	30	4	available	available	ADJ
fnp-5883	30	5	in	in	ADP
fnp-5883	30	6	practical	practical	ADJ
fnp-5883	30	7	brain	brain	NOUN
fnp-5883	30	8	banking	banking	NOUN
fnp-5883	30	9	settings	setting	NOUN
fnp-5883	30	10	for	for	ADP
fnp-5883	30	11	fixation	fixation	NOUN
fnp-5883	30	12	followed	follow	VERB
fnp-5883	30	13	by	by	ADP
fnp-5883	30	14	cryopreservation	cryopreservation	NOUN
fnp-5883	30	15	and	and	CCONJ
fnp-5883	30	16	long	long	ADJ
fnp-5883	30	17	-	-	PUNCT
fnp-5883	30	18	term	term	NOUN
fnp-5883	30	19	storage	storage	NOUN
fnp-5883	30	20	(	(	PUNCT
fnp-5883	30	21	estrada	estrada	PROPN
fnp-5883	30	22	et	et	PROPN
fnp-5883	30	23	al	al	PROPN
fnp-5883	30	24	.	.	PROPN
fnp-5883	30	25	,	,	PUNCT
fnp-5883	30	26	2017	2017	NUM
fnp-5883	30	27	;	;	PUNCT
fnp-5883	30	28	kumarasami	kumarasami	NOUN
fnp-5883	30	29	et	et	PROPN
fnp-5883	30	30	al	al	PROPN
fnp-5883	30	31	.	.	PROPN
fnp-5883	30	32	,	,	PUNCT
fnp-5883	30	33	2023	2023	NUM
fnp-5883	30	34	;	;	PUNCT
fnp-5883	30	35	gasperoni	gasperoni	PROPN
fnp-5883	30	36	and	and	CCONJ
fnp-5883	30	37	dworkin	dworkin	PROPN
fnp-5883	30	38	,	,	PUNCT
fnp-5883	30	39	2024	2024	NUM
fnp-5883	30	40	)	)	PUNCT
fnp-5883	30	41	.	.	PUNCT
fnp-5883	31	1	however	however	ADV
fnp-5883	31	2	,	,	PUNCT
fnp-5883	31	3	for	for	ADP
fnp-5883	31	4	some	some	DET
fnp-5883	31	5	research	research	NOUN
fnp-5883	31	6	applications	application	NOUN
fnp-5883	31	7	,	,	PUNCT
fnp-5883	31	8	fresh	fresh	ADJ
fnp-5883	31	9	tissue	tissue	NOUN
fnp-5883	31	10	is	be	AUX
fnp-5883	31	11	preferable	preferable	ADJ
fnp-5883	31	12	to	to	ADP
fnp-5883	31	13	fixed	fix	VERB
fnp-5883	31	14	tissue	tissue	NOUN
fnp-5883	31	15	.	.	PUNCT
fnp-5883	32	1	therefore	therefore	ADV
fnp-5883	32	2	,	,	PUNCT
fnp-5883	32	3	in	in	ADP
fnp-5883	32	4	this	this	DET
fnp-5883	32	5	review	review	NOUN
fnp-5883	32	6	,	,	PUNCT
fnp-5883	32	7	we	we	PRON
fnp-5883	32	8	focus	focus	VERB
fnp-5883	32	9	on	on	ADP
fnp-5883	32	10	the	the	DET
fnp-5883	32	11	cryopreservation	cryopreservation	NOUN
fnp-5883	32	12	of	of	ADP
fnp-5883	32	13	unfixed	unfixed	ADJ
fnp-5883	32	14	brain	brain	NOUN
fnp-5883	32	15	tissue	tissue	NOUN
fnp-5883	32	16	.	.	PUNCT
fnp-5883	33	1	in	in	ADP
fnp-5883	33	2	this	this	DET
fnp-5883	33	3	review	review	NOUN
fnp-5883	33	4	,	,	PUNCT
fnp-5883	33	5	we	we	PRON
fnp-5883	33	6	examine	examine	VERB
fnp-5883	33	7	existing	exist	VERB
fnp-5883	33	8	data	datum	NOUN
fnp-5883	33	9	on	on	ADP
fnp-5883	33	10	the	the	DET
fnp-5883	33	11	cryopreservation	cryopreservation	NOUN
fnp-5883	33	12	of	of	ADP
fnp-5883	33	13	brain	brain	NOUN
fnp-5883	33	14	tissue	tissue	NOUN
fnp-5883	33	15	,	,	PUNCT
fnp-5883	33	16	identifying	identify	VERB
fnp-5883	33	17	previous	previous	ADJ
fnp-5883	33	18	methods	method	NOUN
fnp-5883	33	19	used	use	VERB
fnp-5883	33	20	and	and	CCONJ
fnp-5883	33	21	their	their	PRON
fnp-5883	33	22	impacts	impact	NOUN
fnp-5883	33	23	on	on	ADP
fnp-5883	33	24	histologic	histologic	ADJ
fnp-5883	33	25	integrity	integrity	NOUN
fnp-5883	33	26	.	.	PUNCT
fnp-5883	34	1	in	in	ADP
fnp-5883	34	2	the	the	DET
fnp-5883	34	3	studies	study	NOUN
fnp-5883	34	4	we	we	PRON
fnp-5883	34	5	identified	identify	VERB
fnp-5883	34	6	,	,	PUNCT
fnp-5883	34	7	the	the	DET
fnp-5883	34	8	most	most	ADV
fnp-5883	34	9	common	common	ADJ
fnp-5883	34	10	long	long	ADJ
fnp-5883	34	11	-	-	PUNCT
fnp-5883	34	12	term	term	NOUN
fnp-5883	34	13	storage	storage	NOUN
fnp-5883	34	14	temperatures	temperature	NOUN
fnp-5883	34	15	range	range	VERB
fnp-5883	34	16	from	from	ADP
fnp-5883	34	17	‑20	‑20	PRON
fnp-5883	34	18	 	 	SPACE
fnp-5883	34	19	°	°	ADP
fnp-5883	34	20	c	c	NOUN
fnp-5883	34	21	to	to	ADP
fnp-5883	34	22	‑196	‑196	PROPN
fnp-5883	34	23	 	 	SPACE
fnp-5883	34	24	°	°	ADP
fnp-5883	34	25	c	c	NOUN
fnp-5883	34	26	(	(	PUNCT
fnp-5883	34	27	liquid	liquid	ADJ
fnp-5883	34	28	nitrogen	nitrogen	NOUN
fnp-5883	34	29	temperature	temperature	NOUN
fnp-5883	34	30	)	)	PUNCT
fnp-5883	34	31	.	.	PUNCT
fnp-5883	35	1	we	we	PRON
fnp-5883	35	2	include	include	VERB
fnp-5883	35	3	approaches	approach	NOUN
fnp-5883	35	4	that	that	PRON
fnp-5883	35	5	attempt	attempt	VERB
fnp-5883	35	6	to	to	PART
fnp-5883	35	7	freeze	freeze	VERB
fnp-5883	35	8	tissue	tissue	NOUN
fnp-5883	35	9	while	while	SCONJ
fnp-5883	35	10	tolerating	tolerate	VERB
fnp-5883	35	11	some	some	DET
fnp-5883	35	12	degree	degree	NOUN
fnp-5883	35	13	of	of	ADP
fnp-5883	35	14	ice	ice	NOUN
fnp-5883	35	15	formation	formation	NOUN
fnp-5883	35	16	as	as	ADV
fnp-5883	35	17	well	well	ADV
fnp-5883	35	18	as	as	ADP
fnp-5883	35	19	approaches	approach	NOUN
fnp-5883	35	20	that	that	PRON
fnp-5883	35	21	attempt	attempt	VERB
fnp-5883	35	22	to	to	PART
fnp-5883	35	23	vitrify	vitrify	VERB
fnp-5883	35	24	tissue	tissue	NOUN
fnp-5883	35	25	and	and	CCONJ
fnp-5883	35	26	thereby	thereby	ADV
fnp-5883	35	27	avoid	avoid	VERB
fnp-5883	35	28	ice	ice	NOUN
fnp-5883	35	29	formation	formation	NOUN
fnp-5883	35	30	.	.	PUNCT
fnp-5883	36	1	we	we	PRON
fnp-5883	36	2	focus	focus	VERB
fnp-5883	36	3	on	on	ADP
fnp-5883	36	4	the	the	DET
fnp-5883	36	5	effects	effect	NOUN
fnp-5883	36	6	of	of	ADP
fnp-5883	36	7	cryopreservation	cryopreservation	NOUN
fnp-5883	36	8	on	on	ADP
fnp-5883	36	9	brain	brain	NOUN
fnp-5883	36	10	structure	structure	NOUN
fnp-5883	36	11	as	as	SCONJ
fnp-5883	36	12	identified	identify	VERB
fnp-5883	36	13	via	via	ADP
fnp-5883	36	14	microscopy	microscopy	NOUN
fnp-5883	36	15	,	,	PUNCT
fnp-5883	36	16	rather	rather	ADV
fnp-5883	36	17	than	than	ADP
fnp-5883	36	18	the	the	DET
fnp-5883	36	19	features	feature	NOUN
fnp-5883	36	20	of	of	ADP
fnp-5883	36	21	brain	brain	NOUN
fnp-5883	36	22	tissue	tissue	NOUN
fnp-5883	36	23	identified	identify	VERB
fnp-5883	36	24	by	by	ADP
fnp-5883	36	25	neuroimaging	neuroimaging	NOUN
fnp-5883	36	26	,	,	PUNCT
fnp-5883	36	27	electrophysiology	electrophysiology	NOUN
fnp-5883	36	28	,	,	PUNCT
fnp-5883	36	29	cell	cell	NOUN
fnp-5883	36	30	culture	culture	NOUN
fnp-5883	36	31	,	,	PUNCT
fnp-5883	36	32	or	or	CCONJ
fnp-5883	36	33	dissociated	dissociate	VERB
fnp-5883	36	34	biomolecular	biomolecular	ADJ
fnp-5883	36	35	profiling	profiling	NOUN
fnp-5883	36	36	.	.	PUNCT
fnp-5883	37	1	we	we	PRON
fnp-5883	37	2	discuss	discuss	VERB
fnp-5883	37	3	the	the	DET
fnp-5883	37	4	mechanisms	mechanism	NOUN
fnp-5883	37	5	of	of	ADP
fnp-5883	37	6	tissue	tissue	NOUN
fnp-5883	37	7	damage	damage	NOUN
fnp-5883	37	8	in	in	ADP
fnp-5883	37	9	cryopreservation	cryopreservation	NOUN
fnp-5883	37	10	,	,	PUNCT
fnp-5883	37	11	the	the	DET
fnp-5883	37	12	efficacy	efficacy	NOUN
fnp-5883	37	13	of	of	ADP
fnp-5883	37	14	different	different	ADJ
fnp-5883	37	15	cooling	cool	VERB
fnp-5883	37	16	methods	method	NOUN
fnp-5883	37	17	,	,	PUNCT
fnp-5883	37	18	the	the	DET
fnp-5883	37	19	use	use	NOUN
fnp-5883	37	20	of	of	ADP
fnp-5883	37	21	chemical	chemical	ADJ
fnp-5883	37	22	cryoprotectants	cryoprotectant	NOUN
fnp-5883	37	23	to	to	PART
fnp-5883	37	24	decrease	decrease	VERB
fnp-5883	37	25	ice	ice	NOUN
fnp-5883	37	26	crystal	crystal	NOUN
fnp-5883	37	27	formation	formation	NOUN
fnp-5883	37	28	,	,	PUNCT
fnp-5883	37	29	and	and	CCONJ
fnp-5883	37	30	other	other	ADJ
fnp-5883	37	31	factors	factor	NOUN
fnp-5883	37	32	that	that	PRON
fnp-5883	37	33	affect	affect	VERB
fnp-5883	37	34	variability	variability	NOUN
fnp-5883	37	35	in	in	ADP
fnp-5883	37	36	cryopreservation	cryopreservation	NOUN
fnp-5883	37	37	outcomes	outcome	NOUN
fnp-5883	37	38	between	between	ADP
fnp-5883	37	39	studies	study	NOUN
fnp-5883	37	40	.	.	PUNCT
fnp-5883	38	1	through	through	ADP
fnp-5883	38	2	this	this	DET
fnp-5883	38	3	review	review	NOUN
fnp-5883	38	4	,	,	PUNCT
fnp-5883	38	5	we	we	PRON
fnp-5883	38	6	aim	aim	VERB
fnp-5883	38	7	to	to	PART
fnp-5883	38	8	illuminate	illuminate	VERB
fnp-5883	38	9	the	the	DET
fnp-5883	38	10	current	current	ADJ
fnp-5883	38	11	landscape	landscape	NOUN
fnp-5883	38	12	of	of	ADP
fnp-5883	38	13	cryopreservation	cryopreservation	NOUN
fnp-5883	38	14	techniques	technique	NOUN
fnp-5883	38	15	,	,	PUNCT
fnp-5883	38	16	identify	identify	VERB
fnp-5883	38	17	gaps	gap	NOUN
fnp-5883	38	18	in	in	ADP
fnp-5883	38	19	knowledge	knowledge	NOUN
fnp-5883	38	20	,	,	PUNCT
fnp-5883	38	21	and	and	CCONJ
fnp-5883	38	22	discuss	discuss	VERB
fnp-5883	38	23	potential	potential	ADJ
fnp-5883	38	24	avenues	avenue	NOUN
fnp-5883	38	25	for	for	ADP
fnp-5883	38	26	improvements	improvement	NOUN
fnp-5883	38	27	.	.	PUNCT
fnp-5883	39	1	this	this	DET
fnp-5883	39	2	review	review	NOUN
fnp-5883	39	3	may	may	AUX
fnp-5883	39	4	be	be	AUX
fnp-5883	39	5	useful	useful	ADJ
fnp-5883	39	6	for	for	ADP
fnp-5883	39	7	individuals	individual	NOUN
fnp-5883	39	8	banking	bank	VERB
fnp-5883	39	9	brain	brain	NOUN
fnp-5883	39	10	tissue	tissue	NOUN
fnp-5883	39	11	via	via	ADP
fnp-5883	39	12	cryopreservation	cryopreservation	NOUN
fnp-5883	39	13	,	,	PUNCT
fnp-5883	39	14	by	by	ADP
fnp-5883	39	15	offering	offer	VERB
fnp-5883	39	16	a	a	DET
fnp-5883	39	17	summary	summary	NOUN
fnp-5883	39	18	of	of	ADP
fnp-5883	39	19	the	the	DET
fnp-5883	39	20	upsides	upside	NOUN
fnp-5883	39	21	and	and	CCONJ
fnp-5883	39	22	downsides	downside	NOUN
fnp-5883	39	23	of	of	ADP
fnp-5883	39	24	the	the	DET
fnp-5883	39	25	different	different	ADJ
fnp-5883	39	26	methods	method	NOUN
fnp-5883	39	27	available	available	ADJ
fnp-5883	39	28	.	.	PUNCT
fnp-5883	40	1	in	in	ADP
fnp-5883	40	2	addition	addition	NOUN
fnp-5883	40	3	,	,	PUNCT
fnp-5883	40	4	researchers	researcher	NOUN
fnp-5883	40	5	who	who	PRON
fnp-5883	40	6	analyze	analyze	VERB
fnp-5883	40	7	cryopreserved	cryopreserved	ADJ
fnp-5883	40	8	brain	brain	NOUN
fnp-5883	40	9	tissue	tissue	NOUN
fnp-5883	40	10	may	may	AUX
fnp-5883	40	11	find	find	VERB
fnp-5883	40	12	this	this	DET
fnp-5883	40	13	review	review	NOUN
fnp-5883	40	14	valuable	valuable	ADJ
fnp-5883	40	15	,	,	PUNCT
fnp-5883	40	16	as	as	SCONJ
fnp-5883	40	17	it	it	PRON
fnp-5883	40	18	summarizes	summarize	VERB
fnp-5883	40	19	our	our	PRON
fnp-5883	40	20	current	current	ADJ
fnp-5883	40	21	knowledge	knowledge	NOUN
fnp-5883	40	22	about	about	ADP
fnp-5883	40	23	the	the	DET
fnp-5883	40	24	expected	expect	VERB
fnp-5883	40	25	burden	burden	NOUN
fnp-5883	40	26	of	of	ADP
fnp-5883	40	27	freezing	freeze	VERB
fnp-5883	40	28	artifacts	artifact	NOUN
fnp-5883	40	29	,	,	PUNCT
fnp-5883	40	30	as	as	ADV
fnp-5883	40	31	well	well	ADV
fnp-5883	40	32	as	as	ADP
fnp-5883	40	33	which	which	DET
fnp-5883	40	34	downstream	downstream	ADJ
fnp-5883	40	35	research	research	NOUN
fnp-5883	40	36	applications	application	NOUN
fnp-5883	40	37	may	may	AUX
fnp-5883	40	38	be	be	AUX
fnp-5883	40	39	the	the	DET
fnp-5883	40	40	most	most	ADV
fnp-5883	40	41	compatible	compatible	ADJ
fnp-5883	40	42	with	with	ADP
fnp-5883	40	43	cryopreserved	cryopreserved	ADJ
fnp-5883	40	44	brain	brain	NOUN
fnp-5883	40	45	tissue	tissue	NOUN
fnp-5883	40	46	.	.	PUNCT
fnp-5883	41	1	methods	method	NOUN
fnp-5883	41	2	realist	realist	ADJ
fnp-5883	41	3	synthesis	synthesis	NOUN
fnp-5883	41	4	we	we	PRON
fnp-5883	41	5	carried	carry	VERB
fnp-5883	41	6	out	out	ADP
fnp-5883	41	7	a	a	DET
fnp-5883	41	8	realist	realist	ADJ
fnp-5883	41	9	synthesis	synthesis	NOUN
fnp-5883	41	10	review	review	NOUN
fnp-5883	41	11	aimed	aim	VERB
fnp-5883	41	12	at	at	ADP
fnp-5883	41	13	establishing	establish	VERB
fnp-5883	41	14	a	a	DET
fnp-5883	41	15	theoretical	theoretical	ADJ
fnp-5883	41	16	understanding	understanding	NOUN
fnp-5883	41	17	of	of	ADP
fnp-5883	41	18	cryopreservation	cryopreservation	NOUN
fnp-5883	41	19	.	.	PUNCT
fnp-5883	42	1	this	this	DET
fnp-5883	42	2	method	method	NOUN
fnp-5883	42	3	integrates	integrate	VERB
fnp-5883	42	4	elements	element	NOUN
fnp-5883	42	5	of	of	ADP
fnp-5883	42	6	a	a	DET
fnp-5883	42	7	systematic	systematic	ADJ
fnp-5883	42	8	review	review	NOUN
fnp-5883	42	9	with	with	ADP
fnp-5883	42	10	an	an	DET
fnp-5883	42	11	emphasis	emphasis	NOUN
fnp-5883	42	12	on	on	ADP
fnp-5883	42	13	theory	theory	NOUN
fnp-5883	42	14	and	and	CCONJ
fnp-5883	42	15	practical	practical	ADJ
fnp-5883	42	16	application	application	NOUN
fnp-5883	42	17	(	(	PUNCT
fnp-5883	42	18	wong	wong	PROPN
fnp-5883	42	19	et	et	PROPN
fnp-5883	42	20	al	al	PROPN
fnp-5883	42	21	.	.	PROPN
fnp-5883	42	22	,	,	PUNCT
fnp-5883	42	23	2013	2013	NUM
fnp-5883	42	24	)	)	PUNCT
fnp-5883	42	25	.	.	PUNCT
fnp-5883	43	1	we	we	PRON
fnp-5883	43	2	chose	choose	VERB
fnp-5883	43	3	this	this	DET
fnp-5883	43	4	approach	approach	NOUN
fnp-5883	43	5	due	due	ADP
fnp-5883	43	6	to	to	ADP
fnp-5883	43	7	the	the	DET
fnp-5883	43	8	wide	wide	ADV
fnp-5883	43	9	-	-	PUNCT
fnp-5883	43	10	ranging	range	VERB
fnp-5883	43	11	methods	method	NOUN
fnp-5883	43	12	used	use	VERB
fnp-5883	43	13	in	in	ADP
fnp-5883	43	14	cryopreservation	cryopreservation	NOUN
fnp-5883	43	15	of	of	ADP
fnp-5883	43	16	brain	brain	NOUN
fnp-5883	43	17	tissue	tissue	NOUN
fnp-5883	43	18	.	.	PUNCT
fnp-5883	44	1	we	we	PRON
fnp-5883	44	2	report	report	VERB
fnp-5883	44	3	our	our	PRON
fnp-5883	44	4	adherence	adherence	NOUN
fnp-5883	44	5	to	to	ADP
fnp-5883	44	6	the	the	DET
fnp-5883	44	7	rameses	ramese	NOUN
fnp-5883	44	8	standards	standard	NOUN
fnp-5883	44	9	(	(	PUNCT
fnp-5883	44	10	supplementary	supplementary	ADJ
fnp-5883	44	11	file	file	NOUN
fnp-5883	44	12	 	 	SPACE
fnp-5883	44	13	1	1	NUM
fnp-5883	44	14	)	)	PUNCT
fnp-5883	44	15	(	(	PUNCT
fnp-5883	44	16	wong	wong	PROPN
fnp-5883	44	17	et	et	PROPN
fnp-5883	44	18	al	al	PROPN
fnp-5883	44	19	.	.	PROPN
fnp-5883	44	20	,	,	PUNCT
fnp-5883	44	21	2013	2013	NUM
fnp-5883	44	22	)	)	PUNCT
fnp-5883	44	23	.	.	PUNCT
fnp-5883	45	1	before	before	ADP
fnp-5883	45	2	formalizing	formalize	VERB
fnp-5883	45	3	our	our	PRON
fnp-5883	45	4	search	search	NOUN
fnp-5883	45	5	methodology	methodology	NOUN
fnp-5883	45	6	,	,	PUNCT
fnp-5883	45	7	we	we	PRON
fnp-5883	45	8	first	first	ADV
fnp-5883	45	9	scoped	scope	VERB
fnp-5883	45	10	the	the	DET
fnp-5883	45	11	literature	literature	NOUN
fnp-5883	45	12	by	by	ADP
fnp-5883	45	13	ad	ad	X
fnp-5883	45	14	hoc	hoc	X
fnp-5883	45	15	searches	search	NOUN
fnp-5883	45	16	of	of	ADP
fnp-5883	45	17	pubmed	pubmed	PROPN
fnp-5883	45	18	,	,	PUNCT
fnp-5883	45	19	google	google	PROPN
fnp-5883	45	20	,	,	PUNCT
fnp-5883	45	21	google	google	NOUN
fnp-5883	45	22	scholar	scholar	NOUN
fnp-5883	45	23	,	,	PUNCT
fnp-5883	45	24	and	and	CCONJ
fnp-5883	45	25	by	by	ADP
fnp-5883	45	26	holding	hold	VERB
fnp-5883	45	27	discussions	discussion	NOUN
fnp-5883	45	28	among	among	ADP
fnp-5883	45	29	the	the	DET
fnp-5883	45	30	authors	author	NOUN
fnp-5883	45	31	.	.	PUNCT
fnp-5883	46	1	the	the	DET
fnp-5883	46	2	review	review	NOUN
fnp-5883	46	3	protocol	protocol	NOUN
fnp-5883	46	4	was	be	AUX
fnp-5883	46	5	preregistered	preregistere	VERB
fnp-5883	46	6	here	here	ADV
fnp-5883	46	7	:	:	PUNCT
fnp-5883	46	8	https://osf.io/y6hv2	https://osf.io/y6hv2	PROPN
fnp-5883	46	9	.	.	PUNCT
fnp-5883	47	1	eligibility	eligibility	NOUN
fnp-5883	47	2	criteria	criterion	NOUN
fnp-5883	47	3	to	to	PART
fnp-5883	47	4	be	be	AUX
fnp-5883	47	5	included	include	VERB
fnp-5883	47	6	in	in	ADP
fnp-5883	47	7	this	this	DET
fnp-5883	47	8	review	review	NOUN
fnp-5883	47	9	,	,	PUNCT
fnp-5883	47	10	studies	study	NOUN
fnp-5883	47	11	needed	need	VERB
fnp-5883	47	12	to	to	PART
fnp-5883	47	13	report	report	VERB
fnp-5883	47	14	original	original	ADJ
fnp-5883	47	15	empirical	empirical	ADJ
fnp-5883	47	16	data	datum	NOUN
fnp-5883	47	17	relevant	relevant	ADJ
fnp-5883	47	18	to	to	ADP
fnp-5883	47	19	the	the	DET
fnp-5883	47	20	effects	effect	NOUN
fnp-5883	47	21	of	of	ADP
fnp-5883	47	22	unfixed	unfixed	ADJ
fnp-5883	47	23	brain	brain	NOUN
fnp-5883	47	24	tissue	tissue	NOUN
fnp-5883	47	25	cryopreservation	cryopreservation	NOUN
fnp-5883	47	26	on	on	ADP
fnp-5883	47	27	histologic	histologic	ADJ
fnp-5883	47	28	integrity	integrity	NOUN
fnp-5883	47	29	.	.	PUNCT
fnp-5883	48	1	we	we	PRON
fnp-5883	48	2	included	include	VERB
fnp-5883	48	3	studies	study	NOUN
fnp-5883	48	4	that	that	PRON
fnp-5883	48	5	met	meet	VERB
fnp-5883	48	6	the	the	DET
fnp-5883	48	7	following	follow	VERB
fnp-5883	48	8	two	two	NUM
fnp-5883	48	9	criteria	criterion	NOUN
fnp-5883	48	10	:	:	PUNCT
fnp-5883	48	11	(	(	PUNCT
fnp-5883	48	12	1	1	X
fnp-5883	48	13	)	)	PUNCT
fnp-5883	48	14	examined	examine	VERB
fnp-5883	48	15	the	the	DET
fnp-5883	48	16	cryopreservation	cryopreservation	NOUN
fnp-5883	48	17	of	of	ADP
fnp-5883	48	18	unfixed	unfixed	ADJ
fnp-5883	48	19	brain	brain	NOUN
fnp-5883	48	20	or	or	CCONJ
fnp-5883	48	21	other	other	ADJ
fnp-5883	48	22	nervous	nervous	ADJ
fnp-5883	48	23	system	system	NOUN
fnp-5883	48	24	tissue	tissue	NOUN
fnp-5883	48	25	.	.	PUNCT
fnp-5883	49	1	isolated	isolated	ADJ
fnp-5883	49	2	cells	cell	NOUN
fnp-5883	49	3	,	,	PUNCT
fnp-5883	49	4	sub	sub	ADJ
fnp-5883	49	5	-	-	ADJ
fnp-5883	49	6	cellular	cellular	ADJ
fnp-5883	49	7	structures	structure	NOUN
fnp-5883	49	8	,	,	PUNCT
fnp-5883	49	9	clusters	cluster	NOUN
fnp-5883	49	10	of	of	ADP
fnp-5883	49	11	cells	cell	NOUN
fnp-5883	49	12	,	,	PUNCT
fnp-5883	49	13	or	or	CCONJ
fnp-5883	49	14	organoids	organoid	NOUN
fnp-5883	49	15	alone	alone	ADV
fnp-5883	49	16	were	be	AUX
fnp-5883	49	17	not	not	PART
fnp-5883	49	18	sufficient	sufficient	ADJ
fnp-5883	49	19	.	.	PUNCT
fnp-5883	50	1	(	(	PUNCT
fnp-5883	50	2	2	2	X
fnp-5883	50	3	)	)	PUNCT
fnp-5883	50	4	assessed	assess	VERB
fnp-5883	50	5	histological	histological	ADJ
fnp-5883	50	6	and/or	and/or	CCONJ
fnp-5883	50	7	morphological	morphological	ADJ
fnp-5883	50	8	preservation	preservation	NOUN
fnp-5883	50	9	through	through	ADP
fnp-5883	50	10	microscopy	microscopy	NOUN
fnp-5883	50	11	or	or	CCONJ
fnp-5883	50	12	other	other	ADJ
fnp-5883	50	13	structural	structural	ADJ
fnp-5883	50	14	analysis	analysis	NOUN
fnp-5883	50	15	methods	method	NOUN
fnp-5883	50	16	.	.	PUNCT
fnp-5883	51	1	through	through	ADP
fnp-5883	51	2	this	this	DET
fnp-5883	51	3	criterion	criterion	NOUN
fnp-5883	51	4	,	,	PUNCT
fnp-5883	51	5	we	we	PRON
fnp-5883	51	6	focused	focus	VERB
fnp-5883	51	7	on	on	ADP
fnp-5883	51	8	structural	structural	ADJ
fnp-5883	51	9	and	and	CCONJ
fnp-5883	51	10	morphological	morphological	ADJ
fnp-5883	51	11	integrity	integrity	NOUN
fnp-5883	51	12	rather	rather	ADV
fnp-5883	51	13	than	than	ADP
fnp-5883	51	14	functional	functional	ADJ
fnp-5883	51	15	or	or	CCONJ
fnp-5883	51	16	biomolecular	biomolecular	ADJ
fnp-5883	51	17	preservation	preservation	NOUN
fnp-5883	51	18	alone	alone	ADV
fnp-5883	51	19	.	.	PUNCT
fnp-5883	52	1	both	both	DET
fnp-5883	52	2	human	human	ADJ
fnp-5883	52	3	and	and	CCONJ
fnp-5883	52	4	non	non	ADJ
fnp-5883	52	5	-	-	ADJ
fnp-5883	52	6	human	human	ADJ
fnp-5883	52	7	animal	animal	NOUN
fnp-5883	52	8	studies	study	NOUN
fnp-5883	52	9	were	be	AUX
fnp-5883	52	10	eligible	eligible	ADJ
fnp-5883	52	11	for	for	ADP
fnp-5883	52	12	inclusion	inclusion	NOUN
fnp-5883	52	13	.	.	PUNCT
fnp-5883	53	1	if	if	SCONJ
fnp-5883	53	2	multiple	multiple	ADJ
fnp-5883	53	3	publications	publication	NOUN
fnp-5883	53	4	report	report	VERB
fnp-5883	53	5	on	on	ADP
fnp-5883	53	6	the	the	DET
fnp-5883	53	7	same	same	ADJ
fnp-5883	53	8	underlying	underlying	ADJ
fnp-5883	53	9	data	datum	NOUN
fnp-5883	53	10	,	,	PUNCT
fnp-5883	53	11	we	we	PRON
fnp-5883	53	12	only	only	ADV
fnp-5883	53	13	included	include	VERB
fnp-5883	53	14	the	the	DET
fnp-5883	53	15	study	study	NOUN
fnp-5883	53	16	that	that	PRON
fnp-5883	53	17	drew	draw	VERB
fnp-5883	53	18	the	the	DET
fnp-5883	53	19	most	most	ADV
fnp-5883	53	20	relevant	relevant	ADJ
fnp-5883	53	21	conclusions	conclusion	NOUN
fnp-5883	53	22	about	about	ADP
fnp-5883	53	23	the	the	DET
fnp-5883	53	24	effects	effect	NOUN
fnp-5883	53	25	on	on	ADP
fnp-5883	53	26	cryopreservation	cryopreservation	NOUN
fnp-5883	53	27	.	.	PUNCT
fnp-5883	54	1	when	when	SCONJ
fnp-5883	54	2	a	a	DET
fnp-5883	54	3	single	single	ADJ
fnp-5883	54	4	study	study	NOUN
fnp-5883	54	5	reported	report	VERB
fnp-5883	54	6	multiple	multiple	ADJ
fnp-5883	54	7	independent	independent	ADJ
fnp-5883	54	8	observations	observation	NOUN
fnp-5883	54	9	about	about	ADP
fnp-5883	54	10	the	the	DET
fnp-5883	54	11	effects	effect	NOUN
fnp-5883	54	12	of	of	ADP
fnp-5883	54	13	cryopreservation	cryopreservation	NOUN
fnp-5883	54	14	,	,	PUNCT
fnp-5883	54	15	such	such	ADJ
fnp-5883	54	16	as	as	ADP
fnp-5883	54	17	a	a	DET
fnp-5883	54	18	different	different	ADJ
fnp-5883	54	19	type	type	NOUN
fnp-5883	54	20	of	of	ADP
fnp-5883	54	21	brain	brain	NOUN
fnp-5883	54	22	sample	sample	NOUN
fnp-5883	54	23	,	,	PUNCT
fnp-5883	54	24	cooling	cool	VERB
fnp-5883	54	25	rate	rate	NOUN
fnp-5883	54	26	,	,	PUNCT
fnp-5883	54	27	or	or	CCONJ
fnp-5883	54	28	rewarming	rewarming	NOUN
fnp-5883	54	29	method	method	NOUN
fnp-5883	54	30	,	,	PUNCT
fnp-5883	54	31	they	they	PRON
fnp-5883	54	32	were	be	AUX
fnp-5883	54	33	each	each	PRON
fnp-5883	54	34	extracted	extract	VERB
fnp-5883	54	35	from	from	ADP
fnp-5883	54	36	the	the	DET
fnp-5883	54	37	study	study	NOUN
fnp-5883	54	38	as	as	ADP
fnp-5883	54	39	separate	separate	ADJ
fnp-5883	54	40	data	datum	NOUN
fnp-5883	54	41	points	point	NOUN
fnp-5883	54	42	.	.	PUNCT
fnp-5883	55	1	search	search	NOUN
fnp-5883	55	2	methods	method	NOUN
fnp-5883	55	3	and	and	CCONJ
fnp-5883	55	4	study	study	NOUN
fnp-5883	55	5	selection	selection	NOUN
fnp-5883	55	6	we	we	PRON
fnp-5883	55	7	searched	search	VERB
fnp-5883	55	8	pubmed	pubmed	PROPN
fnp-5883	55	9	to	to	PART
fnp-5883	55	10	identify	identify	VERB
fnp-5883	55	11	relevant	relevant	ADJ
fnp-5883	55	12	studies	study	NOUN
fnp-5883	55	13	with	with	ADP
fnp-5883	55	14	keywords	keyword	NOUN
fnp-5883	55	15	related	relate	VERB
fnp-5883	55	16	to	to	ADP
fnp-5883	55	17	cryopreservation	cryopreservation	NOUN
fnp-5883	55	18	of	of	ADP
fnp-5883	55	19	brain	brain	NOUN
fnp-5883	55	20	tissue	tissue	NOUN
fnp-5883	55	21	.	.	PUNCT
fnp-5883	56	1	we	we	PRON
fnp-5883	56	2	searched	search	VERB
fnp-5883	56	3	the	the	DET
fnp-5883	56	4	pubmed	pubmed	PROPN
fnp-5883	56	5	database	database	NOUN
fnp-5883	56	6	from	from	ADP
fnp-5883	56	7	its	its	PRON
fnp-5883	56	8	inception	inception	NOUN
fnp-5883	56	9	to	to	ADP
fnp-5883	56	10	april	april	PROPN
fnp-5883	56	11	18th	18th	NOUN
fnp-5883	56	12	,	,	PUNCT
fnp-5883	56	13	2024	2024	NUM
fnp-5883	56	14	,	,	PUNCT
fnp-5883	56	15	using	use	VERB
fnp-5883	56	16	the	the	DET
fnp-5883	56	17	following	follow	VERB
fnp-5883	56	18	search	search	NOUN
fnp-5883	56	19	query	query	NOUN
fnp-5883	56	20	:	:	PUNCT
fnp-5883	56	21	«	«	PUNCT
fnp-5883	56	22	(	(	PUNCT
fnp-5883	56	23	freezing[tiab	freezing[tiab	X
fnp-5883	56	24	]	]	PUNCT
fnp-5883	56	25	or	or	CCONJ
fnp-5883	56	26	ice[tiab	ice[tiab	NOUN
fnp-5883	56	27	]	]	PUNCT
fnp-5883	56	28	or	or	CCONJ
fnp-5883	56	29	cryopreservation[tiab	cryopreservation[tiab	NOUN
fnp-5883	56	30	]	]	PUNCT
fnp-5883	56	31	or	or	CCONJ
fnp-5883	56	32	"	"	PUNCT
fnp-5883	56	33	cryopreservation"[mesh	cryopreservation"[mesh	PROPN
fnp-5883	56	34	]	]	PUNCT
fnp-5883	56	35	)	)	PUNCT
fnp-5883	56	36	and	and	CCONJ
fnp-5883	56	37	(	(	PUNCT
fnp-5883	56	38	histology[tiab	histology[tiab	X
fnp-5883	56	39	]	]	PUNCT
fnp-5883	56	40	or	or	CCONJ
fnp-5883	56	41	microscopy[tiab	microscopy[tiab	NOUN
fnp-5883	56	42	]	]	PUNCT
fnp-5883	56	43	or	or	CCONJ
fnp-5883	56	44	ultrastructure[tiab	ultrastructure[tiab	NOUN
fnp-5883	56	45	]	]	PUNCT
fnp-5883	56	46	or	or	CCONJ
fnp-5883	56	47	"	"	PUNCT
fnp-5883	56	48	electron	electron	NOUN
fnp-5883	56	49	microscopy"[tiab	microscopy"[tiab	PROPN
fnp-5883	56	50	]	]	PUNCT
fnp-5883	56	51	or	or	CCONJ
fnp-5883	56	52	"	"	PUNCT
fnp-5883	56	53	ultrastructure"[subheading	ultrastructure"[subheading	PROPN
fnp-5883	56	54	]	]	PUNCT
fnp-5883	56	55	)	)	PUNCT
fnp-5883	56	56	and	and	CCONJ
fnp-5883	56	57	(	(	PUNCT
fnp-5883	56	58	brain[tiab	brain[tiab	NOUN
fnp-5883	56	59	]	]	PUNCT
fnp-5883	56	60	or	or	CCONJ
fnp-5883	56	61	"	"	PUNCT
fnp-5883	56	62	central	central	ADJ
fnp-5883	56	63	nervous	nervous	ADJ
fnp-5883	56	64	system"[tiab	system"[tiab	PROPN
fnp-5883	56	65	]	]	PUNCT
fnp-5883	56	66	or	or	CCONJ
fnp-5883	56	67	"	"	PUNCT
fnp-5883	56	68	brain"[mesh	brain"[mesh	PROPN
fnp-5883	56	69	]	]	PUNCT
fnp-5883	56	70	)	)	PUNCT
fnp-5883	56	71	»	»	X
fnp-5883	56	72	.	.	PUNCT
fnp-5883	57	1	one	one	NUM
fnp-5883	57	2	reviewer	reviewer	NOUN
fnp-5883	57	3	screened	screen	VERB
fnp-5883	57	4	the	the	DET
fnp-5883	57	5	titles	title	NOUN
fnp-5883	57	6	and	and	CCONJ
fnp-5883	57	7	abstracts	abstract	NOUN
fnp-5883	57	8	and	and	CCONJ
fnp-5883	57	9	identified	identify	VERB
fnp-5883	57	10	studies	study	NOUN
fnp-5883	57	11	for	for	ADP
fnp-5883	57	12	full	full	ADJ
fnp-5883	57	13	-	-	PUNCT
fnp-5883	57	14	text	text	NOUN
fnp-5883	57	15	review	review	NOUN
fnp-5883	57	16	based	base	VERB
fnp-5883	57	17	on	on	ADP
fnp-5883	57	18	the	the	DET
fnp-5883	57	19	eligibility	eligibility	NOUN
fnp-5883	57	20	criteria	criterion	NOUN
fnp-5883	57	21	(	(	PUNCT
fnp-5883	57	22	supplementary	supplementary	ADJ
fnp-5883	57	23	file	file	NOUN
fnp-5883	57	24	 	 	SPACE
fnp-5883	57	25	2	2	NUM
fnp-5883	57	26	)	)	PUNCT
fnp-5883	57	27	.	.	PUNCT
fnp-5883	58	1	next	next	ADV
fnp-5883	58	2	,	,	PUNCT
fnp-5883	58	3	one	one	NUM
fnp-5883	58	4	reviewer	reviewer	NOUN
fnp-5883	58	5	evaluated	evaluate	VERB
fnp-5883	58	6	the	the	DET
fnp-5883	58	7	full	full	ADJ
fnp-5883	58	8	texts	text	NOUN
fnp-5883	58	9	and	and	CCONJ
fnp-5883	58	10	selected	select	VERB
fnp-5883	58	11	studies	study	NOUN
fnp-5883	58	12	to	to	PART
fnp-5883	58	13	include	include	VERB
fnp-5883	58	14	(	(	PUNCT
fnp-5883	58	15	supplementary	supplementary	ADJ
fnp-5883	58	16	file	file	NOUN
fnp-5883	58	17	 	 	SPACE
fnp-5883	58	18	3	3	NUM
fnp-5883	58	19	)	)	PUNCT
fnp-5883	58	20	.	.	PUNCT
fnp-5883	59	1	to	to	PART
fnp-5883	59	2	find	find	VERB
fnp-5883	59	3	additional	additional	ADJ
fnp-5883	59	4	eligible	eligible	ADJ
fnp-5883	59	5	studies	study	NOUN
fnp-5883	59	6	,	,	PUNCT
fnp-5883	59	7	we	we	PRON
fnp-5883	59	8	also	also	ADV
fnp-5883	59	9	(	(	PUNCT
fnp-5883	59	10	a	a	X
fnp-5883	59	11	)	)	PUNCT
fnp-5883	59	12	hand	hand	NOUN
fnp-5883	59	13	-	-	PUNCT
fnp-5883	59	14	searched	search	VERB
fnp-5883	59	15	the	the	DET
fnp-5883	59	16	reference	reference	NOUN
fnp-5883	59	17	lists	list	NOUN
fnp-5883	59	18	and	and	CCONJ
fnp-5883	59	19	referencing	reference	VERB
fnp-5883	59	20	studies	study	NOUN
fnp-5883	59	21	of	of	ADP
fnp-5883	59	22	the	the	DET
fnp-5883	59	23	most	most	ADV
fnp-5883	59	24	relevant	relevant	ADJ
fnp-5883	59	25	papers	paper	NOUN
fnp-5883	59	26	and	and	CCONJ
fnp-5883	59	27	(	(	PUNCT
fnp-5883	59	28	b	b	X
fnp-5883	59	29	)	)	PUNCT
fnp-5883	59	30	performed	perform	VERB
fnp-5883	59	31	additional	additional	ADJ
fnp-5883	59	32	ad	ad	X
fnp-5883	59	33	hoc	hoc	X
fnp-5883	59	34	searches	search	NOUN
fnp-5883	59	35	of	of	ADP
fnp-5883	59	36	the	the	DET
fnp-5883	59	37	literature	literature	NOUN
fnp-5883	59	38	relevant	relevant	ADJ
fnp-5883	59	39	to	to	ADP
fnp-5883	59	40	this	this	DET
fnp-5883	59	41	topic	topic	NOUN
fnp-5883	59	42	.	.	PUNCT
fnp-5883	60	1	any	any	DET
fnp-5883	60	2	studies	study	NOUN
fnp-5883	60	3	meeting	meet	VERB
fnp-5883	60	4	the	the	DET
fnp-5883	60	5	inclusion	inclusion	NOUN
fnp-5883	60	6	criteria	criterion	NOUN
fnp-5883	60	7	that	that	PRON
fnp-5883	60	8	were	be	AUX
fnp-5883	60	9	identified	identify	VERB
fnp-5883	60	10	outside	outside	ADP
fnp-5883	60	11	the	the	DET
fnp-5883	60	12	formal	formal	ADJ
fnp-5883	60	13	search	search	NOUN
fnp-5883	60	14	process	process	NOUN
fnp-5883	60	15	,	,	PUNCT
fnp-5883	60	16	such	such	ADJ
fnp-5883	60	17	as	as	ADP
fnp-5883	60	18	in	in	ADP
fnp-5883	60	19	the	the	DET
fnp-5883	60	20	initial	initial	ADJ
fnp-5883	60	21	literature	literature	NOUN
fnp-5883	60	22	scoping	scoping	NOUN
fnp-5883	60	23	process	process	NOUN
fnp-5883	60	24	,	,	PUNCT
fnp-5883	60	25	were	be	AUX
fnp-5883	60	26	also	also	ADV
fnp-5883	60	27	included	include	VERB
fnp-5883	60	28	.	.	PUNCT
fnp-5883	61	1	data	datum	NOUN
fnp-5883	61	2	extraction	extraction	NOUN
fnp-5883	61	3	for	for	ADP
fnp-5883	61	4	each	each	DET
fnp-5883	61	5	included	include	VERB
fnp-5883	61	6	study	study	NOUN
fnp-5883	61	7	,	,	PUNCT
fnp-5883	61	8	we	we	PRON
fnp-5883	61	9	extracted	extract	VERB
fnp-5883	61	10	relevant	relevant	ADJ
fnp-5883	61	11	data	datum	NOUN
fnp-5883	61	12	:	:	PUNCT
fnp-5883	61	13	species	specie	NOUN
fnp-5883	61	14	;	;	PUNCT
fnp-5883	61	15	specimen	speciman	NOUN
fnp-5883	61	16	type	type	NOUN
fnp-5883	61	17	;	;	PUNCT
fnp-5883	61	18	cryoprotectant	cryoprotectant	NOUN
fnp-5883	61	19	,	,	PUNCT
fnp-5883	61	20	if	if	SCONJ
fnp-5883	61	21	any	any	PRON
fnp-5883	61	22	reported	report	VERB
fnp-5883	61	23	;	;	PUNCT
fnp-5883	61	24	cooling	cool	VERB
fnp-5883	61	25	method	method	NOUN
fnp-5883	61	26	;	;	PUNCT
fnp-5883	61	27	storage	storage	NOUN
fnp-5883	61	28	temperature	temperature	NOUN
fnp-5883	61	29	;	;	PUNCT
fnp-5883	61	30	storage	storage	NOUN
fnp-5883	61	31	length	length	NOUN
fnp-5883	61	32	;	;	PUNCT
fnp-5883	61	33	rewarming	rewarming	NOUN
fnp-5883	61	34	method	method	NOUN
fnp-5883	61	35	;	;	PUNCT
fnp-5883	61	36	visualization	visualization	NOUN
fnp-5883	61	37	method	method	NOUN
fnp-5883	61	38	;	;	PUNCT
fnp-5883	61	39	any	any	DET
fnp-5883	61	40	additional	additional	ADJ
fnp-5883	61	41	outcome	outcome	NOUN
fnp-5883	61	42	specifier	specifi	ADJ
fnp-5883	61	43	reported	report	VERB
fnp-5883	61	44	;	;	PUNCT
fnp-5883	61	45	the	the	DET
fnp-5883	61	46	histological	histological	ADJ
fnp-5883	61	47	or	or	CCONJ
fnp-5883	61	48	morphological	morphological	ADJ
fnp-5883	61	49	outcome(s	outcome(s	NOUN
fnp-5883	61	50	)	)	PUNCT
fnp-5883	61	51	described	describe	VERB
fnp-5883	61	52	;	;	PUNCT
fnp-5883	61	53	and	and	CCONJ
fnp-5883	61	54	any	any	DET
fnp-5883	61	55	relevant	relevant	ADJ
fnp-5883	61	56	quote	quote	NOUN
fnp-5883	61	57	or	or	CCONJ
fnp-5883	61	58	quotes	quote	NOUN
fnp-5883	61	59	.	.	PUNCT
fnp-5883	62	1	we	we	PRON
fnp-5883	62	2	treated	treat	VERB
fnp-5883	62	3	each	each	DET
fnp-5883	62	4	distinct	distinct	ADJ
fnp-5883	62	5	cryopreservation	cryopreservation	NOUN
fnp-5883	62	6	condition	condition	NOUN
fnp-5883	62	7	,	,	PUNCT
fnp-5883	62	8	tissue	tissue	NOUN
fnp-5883	62	9	type	type	NOUN
fnp-5883	62	10	,	,	PUNCT
fnp-5883	62	11	visualization	visualization	NOUN
fnp-5883	62	12	method	method	NOUN
fnp-5883	62	13	,	,	PUNCT
fnp-5883	62	14	or	or	CCONJ
fnp-5883	62	15	other	other	ADJ
fnp-5883	62	16	methodologic	methodologic	ADJ
fnp-5883	62	17	difference	difference	NOUN
fnp-5883	62	18	reported	report	VERB
fnp-5883	62	19	in	in	ADP
fnp-5883	62	20	a	a	DET
fnp-5883	62	21	study	study	NOUN
fnp-5883	62	22	as	as	ADP
fnp-5883	62	23	a	a	DET
fnp-5883	62	24	separate	separate	ADJ
fnp-5883	62	25	“	"	PUNCT
fnp-5883	62	26	observation	observation	NOUN
fnp-5883	62	27	”	"	PUNCT
fnp-5883	62	28	.	.	PUNCT
fnp-5883	63	1	for	for	ADP
fnp-5883	63	2	example	example	NOUN
fnp-5883	63	3	,	,	PUNCT
fnp-5883	63	4	if	if	SCONJ
fnp-5883	63	5	a	a	DET
fnp-5883	63	6	study	study	NOUN
fnp-5883	63	7	reported	report	VERB
fnp-5883	63	8	outcomes	outcome	NOUN
fnp-5883	63	9	for	for	ADP
fnp-5883	63	10	tissue	tissue	NOUN
fnp-5883	63	11	cooled	cool	VERB
fnp-5883	63	12	at	at	ADP
fnp-5883	63	13	two	two	NUM
fnp-5883	63	14	different	different	ADJ
fnp-5883	63	15	rates	rate	NOUN
fnp-5883	63	16	,	,	PUNCT
fnp-5883	63	17	these	these	PRON
fnp-5883	63	18	were	be	AUX
fnp-5883	63	19	recorded	record	VERB
fnp-5883	63	20	as	as	ADP
fnp-5883	63	21	two	two	NUM
fnp-5883	63	22	observations	observation	NOUN
fnp-5883	63	23	.	.	PUNCT
fnp-5883	64	1	however	however	ADV
fnp-5883	64	2	,	,	PUNCT
fnp-5883	64	3	minor	minor	ADJ
fnp-5883	64	4	variations	variation	NOUN
fnp-5883	64	5	in	in	ADP
fnp-5883	64	6	otherwise	otherwise	ADV
fnp-5883	64	7	highly	highly	ADV
fnp-5883	64	8	similar	similar	ADJ
fnp-5883	64	9	cryopreservation	cryopreservation	NOUN
fnp-5883	64	10	conditions	condition	NOUN
fnp-5883	64	11	or	or	CCONJ
fnp-5883	64	12	visualization	visualization	NOUN
fnp-5883	64	13	methods	method	NOUN
fnp-5883	64	14	from	from	ADP
fnp-5883	64	15	the	the	DET
fnp-5883	64	16	same	same	ADJ
fnp-5883	64	17	study	study	NOUN
fnp-5883	64	18	were	be	AUX
fnp-5883	64	19	grouped	group	VERB
fnp-5883	64	20	into	into	ADP
fnp-5883	64	21	a	a	DET
fnp-5883	64	22	single	single	ADJ
fnp-5883	64	23	observation	observation	NOUN
fnp-5883	64	24	.	.	PUNCT
fnp-5883	65	1	the	the	DET
fnp-5883	65	2	determination	determination	NOUN
fnp-5883	65	3	of	of	ADP
fnp-5883	65	4	whether	whether	SCONJ
fnp-5883	65	5	two	two	NUM
fnp-5883	65	6	similar	similar	ADJ
fnp-5883	65	7	cryopreservation	cryopreservation	NOUN
fnp-5883	65	8	conditions	condition	NOUN
fnp-5883	65	9	or	or	CCONJ
fnp-5883	65	10	visualization	visualization	NOUN
fnp-5883	65	11	methods	method	NOUN
fnp-5883	65	12	should	should	AUX
fnp-5883	65	13	be	be	AUX
fnp-5883	65	14	grouped	group	VERB
fnp-5883	65	15	into	into	ADP
fnp-5883	65	16	one	one	NUM
fnp-5883	65	17	observation	observation	NOUN
fnp-5883	65	18	or	or	CCONJ
fnp-5883	65	19	separated	separate	VERB
fnp-5883	65	20	was	be	AUX
fnp-5883	65	21	based	base	VERB
fnp-5883	65	22	on	on	ADP
fnp-5883	65	23	an	an	DET
fnp-5883	65	24	assessment	assessment	NOUN
fnp-5883	65	25	of	of	ADP
fnp-5883	65	26	the	the	DET
fnp-5883	65	27	level	level	NOUN
fnp-5883	65	28	of	of	ADP
fnp-5883	65	29	detail	detail	NOUN
fnp-5883	65	30	and	and	CCONJ
fnp-5883	65	31	independence	independence	NOUN
fnp-5883	65	32	reported	report	VERB
fnp-5883	65	33	for	for	ADP
fnp-5883	65	34	each	each	PRON
fnp-5883	65	35	in	in	ADP
fnp-5883	65	36	the	the	DET
fnp-5883	65	37	text	text	NOUN
fnp-5883	65	38	.	.	PUNCT
fnp-5883	66	1	for	for	ADP
fnp-5883	66	2	each	each	DET
fnp-5883	66	3	study	study	NOUN
fnp-5883	66	4	,	,	PUNCT
fnp-5883	66	5	one	one	NUM
fnp-5883	66	6	reviewer	reviewer	NOUN
fnp-5883	66	7	performed	perform	VERB
fnp-5883	66	8	the	the	DET
fnp-5883	66	9	data	datum	NOUN
fnp-5883	66	10	extraction	extraction	NOUN
fnp-5883	66	11	.	.	PUNCT
fnp-5883	67	1	on	on	ADP
fnp-5883	67	2	randomly	randomly	ADV
fnp-5883	67	3	selected	select	VERB
fnp-5883	67	4	articles	article	NOUN
fnp-5883	67	5	,	,	PUNCT
fnp-5883	67	6	chosen	choose	VERB
fnp-5883	67	7	via	via	ADP
fnp-5883	67	8	https://www.random.org/	https://www.random.org/	NOUN
fnp-5883	67	9	,	,	PUNCT
fnp-5883	67	10	one	one	NUM
fnp-5883	67	11	other	other	ADJ
fnp-5883	67	12	reviewer	reviewer	NOUN
fnp-5883	67	13	then	then	ADV
fnp-5883	67	14	evaluated	evaluate	VERB
fnp-5883	67	15	the	the	DET
fnp-5883	67	16	extracted	extract	VERB
fnp-5883	67	17	data	datum	NOUN
fnp-5883	67	18	.	.	PUNCT
fnp-5883	68	1	this	this	DET
fnp-5883	68	2	independent	independent	ADJ
fnp-5883	68	3	evaluation	evaluation	NOUN
fnp-5883	68	4	process	process	NOUN
fnp-5883	68	5	was	be	AUX
fnp-5883	68	6	performed	perform	VERB
fnp-5883	68	7	on	on	ADP
fnp-5883	68	8	n	n	CCONJ
fnp-5883	68	9	 	 	SPACE
fnp-5883	68	10	=	=	NOUN
fnp-5883	68	11	 	 	SPACE
fnp-5883	68	12	20	20	NUM
fnp-5883	68	13	(	(	PUNCT
fnp-5883	68	14	21	21	NUM
fnp-5883	68	15	 	 	SPACE
fnp-5883	68	16	%	%	NOUN
fnp-5883	68	17	)	)	PUNCT
fnp-5883	68	18	of	of	ADP
fnp-5883	68	19	the	the	DET
fnp-5883	68	20	studies	study	NOUN
fnp-5883	68	21	and	and	CCONJ
fnp-5883	68	22	did	do	AUX
fnp-5883	68	23	not	not	PART
fnp-5883	68	24	identify	identify	VERB
fnp-5883	68	25	any	any	DET
fnp-5883	68	26	major	major	ADJ
fnp-5883	68	27	discrepancies	discrepancy	NOUN
fnp-5883	68	28	in	in	ADP
fnp-5883	68	29	the	the	DET
fnp-5883	68	30	extracted	extract	VERB
fnp-5883	68	31	data	datum	NOUN
fnp-5883	68	32	.	.	PUNCT
fnp-5883	69	1	the	the	DET
fnp-5883	69	2	extracted	extract	VERB
fnp-5883	69	3	data	data	NOUN
fnp-5883	69	4	table	table	NOUN
fnp-5883	69	5	is	be	AUX
fnp-5883	69	6	available	available	ADJ
fnp-5883	69	7	here	here	ADV
fnp-5883	69	8	:	:	PUNCT
fnp-5883	69	9	https://github.com/andymckenzie/cryopreservation_review	https://github.com/andymckenzie/cryopreservation_review	NOUN
fnp-5883	69	10	.	.	PUNCT
fnp-5883	70	1	qualitative	qualitative	ADJ
fnp-5883	70	2	data	datum	NOUN
fnp-5883	70	3	analysis	analysis	NOUN
fnp-5883	70	4	due	due	ADP
fnp-5883	70	5	to	to	ADP
fnp-5883	70	6	the	the	DET
fnp-5883	70	7	wide	wide	ADJ
fnp-5883	70	8	range	range	NOUN
fnp-5883	70	9	of	of	ADP
fnp-5883	70	10	cryopreservation	cryopreservation	NOUN
fnp-5883	70	11	techniques	technique	NOUN
fnp-5883	70	12	and	and	CCONJ
fnp-5883	70	13	outcomes	outcome	NOUN
fnp-5883	70	14	reported	report	VERB
fnp-5883	70	15	,	,	PUNCT
fnp-5883	70	16	we	we	PRON
fnp-5883	70	17	performed	perform	VERB
fnp-5883	70	18	a	a	DET
fnp-5883	70	19	qualitative	qualitative	ADJ
fnp-5883	70	20	synthesis	synthesis	NOUN
fnp-5883	70	21	rather	rather	ADV
fnp-5883	70	22	than	than	ADP
fnp-5883	70	23	attempting	attempt	VERB
fnp-5883	70	24	formal	formal	ADJ
fnp-5883	70	25	quantitative	quantitative	ADJ
fnp-5883	70	26	meta	meta	ADJ
fnp-5883	70	27	-	-	PUNCT
fnp-5883	70	28	analysis	analysis	NOUN
fnp-5883	70	29	or	or	CCONJ
fnp-5883	70	30	grading	grading	NOUN
fnp-5883	70	31	of	of	ADP
fnp-5883	70	32	outcomes	outcome	NOUN
fnp-5883	70	33	.	.	PUNCT
fnp-5883	71	1	we	we	PRON
fnp-5883	71	2	drew	draw	VERB
fnp-5883	71	3	insights	insight	NOUN
fnp-5883	71	4	from	from	ADP
fnp-5883	71	5	the	the	DET
fnp-5883	71	6	extracted	extract	VERB
fnp-5883	71	7	data	datum	NOUN
fnp-5883	71	8	to	to	PART
fnp-5883	71	9	categorize	categorize	VERB
fnp-5883	71	10	studies	study	NOUN
fnp-5883	71	11	and	and	CCONJ
fnp-5883	71	12	identify	identify	VERB
fnp-5883	71	13	key	key	ADJ
fnp-5883	71	14	themes	theme	NOUN
fnp-5883	71	15	.	.	PUNCT
fnp-5883	72	1	we	we	PRON
fnp-5883	72	2	categorized	categorize	VERB
fnp-5883	72	3	the	the	DET
fnp-5883	72	4	included	include	VERB
fnp-5883	72	5	studies	study	NOUN
fnp-5883	72	6	into	into	ADP
fnp-5883	72	7	three	three	NUM
fnp-5883	72	8	partially	partially	ADV
fnp-5883	72	9	overlapping	overlap	VERB
fnp-5883	72	10	types	type	NOUN
fnp-5883	72	11	based	base	VERB
fnp-5883	72	12	on	on	ADP
fnp-5883	72	13	the	the	DET
fnp-5883	72	14	cryopreservation	cryopreservation	NOUN
fnp-5883	72	15	approach	approach	NOUN
fnp-5883	72	16	used	use	VERB
fnp-5883	72	17	:	:	PUNCT
fnp-5883	72	18	(	(	PUNCT
fnp-5883	72	19	1	1	X
fnp-5883	72	20	)	)	PUNCT
fnp-5883	72	21	cryofixation	cryofixation	NOUN
fnp-5883	72	22	,	,	PUNCT
fnp-5883	72	23	(	(	PUNCT
fnp-5883	72	24	2	2	X
fnp-5883	72	25	)	)	PUNCT
fnp-5883	72	26	freezing	freezing	NOUN
fnp-5883	72	27	,	,	PUNCT
fnp-5883	72	28	and	and	CCONJ
fnp-5883	72	29	(	(	PUNCT
fnp-5883	72	30	3	3	X
fnp-5883	72	31	)	)	PUNCT
fnp-5883	72	32	cryopreservation	cryopreservation	NOUN
fnp-5883	72	33	with	with	ADP
fnp-5883	72	34	cryoprotectants	cryoprotectant	NOUN
fnp-5883	72	35	.	.	PUNCT
fnp-5883	73	1	we	we	PRON
fnp-5883	73	2	focused	focus	VERB
fnp-5883	73	3	on	on	ADP
fnp-5883	73	4	comparing	compare	VERB
fnp-5883	73	5	histologic	histologic	ADJ
fnp-5883	73	6	outcomes	outcome	NOUN
fnp-5883	73	7	between	between	ADP
fnp-5883	73	8	different	different	ADJ
fnp-5883	73	9	methods	method	NOUN
fnp-5883	73	10	of	of	ADP
fnp-5883	73	11	cryopreserving	cryopreserve	VERB
fnp-5883	73	12	brain	brain	NOUN
fnp-5883	73	13	tissue	tissue	NOUN
fnp-5883	73	14	,	,	PUNCT
fnp-5883	73	15	as	as	ADV
fnp-5883	73	16	well	well	ADV
fnp-5883	73	17	as	as	ADP
fnp-5883	73	18	identifying	identify	VERB
fnp-5883	73	19	mechanistic	mechanistic	ADJ
fnp-5883	73	20	insights	insight	NOUN
fnp-5883	73	21	into	into	ADP
fnp-5883	73	22	the	the	DET
fnp-5883	73	23	causes	cause	NOUN
fnp-5883	73	24	of	of	ADP
fnp-5883	73	25	these	these	DET
fnp-5883	73	26	outcomes	outcome	NOUN
fnp-5883	73	27	.	.	PUNCT
fnp-5883	74	1	example	example	NOUN
fnp-5883	74	2	frozen	frozen	ADJ
fnp-5883	74	3	brain	brain	NOUN
fnp-5883	74	4	tissue	tissue	NOUN
fnp-5883	74	5	data	data	VERB
fnp-5883	74	6	the	the	DET
fnp-5883	74	7	brain	brain	NOUN
fnp-5883	74	8	tissue	tissue	NOUN
fnp-5883	74	9	used	use	VERB
fnp-5883	74	10	was	be	AUX
fnp-5883	74	11	obtained	obtain	VERB
fnp-5883	74	12	and	and	CCONJ
fnp-5883	74	13	de	de	VERB
fnp-5883	74	14	-	-	VERB
fnp-5883	74	15	identified	identify	VERB
fnp-5883	74	16	at	at	ADP
fnp-5883	74	17	the	the	DET
fnp-5883	74	18	icahn	icahn	PROPN
fnp-5883	74	19	school	school	PROPN
fnp-5883	74	20	of	of	ADP
fnp-5883	74	21	medicine	medicine	NOUN
fnp-5883	74	22	at	at	ADP
fnp-5883	74	23	mount	mount	PROPN
fnp-5883	74	24	sinai	sinai	PROPN
fnp-5883	74	25	in	in	ADP
fnp-5883	74	26	accordance	accordance	NOUN
fnp-5883	74	27	with	with	ADP
fnp-5883	74	28	its	its	PRON
fnp-5883	74	29	policies	policy	NOUN
fnp-5883	74	30	,	,	PUNCT
fnp-5883	74	31	regulations	regulation	NOUN
fnp-5883	74	32	,	,	PUNCT
fnp-5883	74	33	and	and	CCONJ
fnp-5883	74	34	institutional	institutional	ADJ
fnp-5883	74	35	review	review	NOUN
fnp-5883	74	36	board	board	NOUN
fnp-5883	74	37	recommendations	recommendation	NOUN
fnp-5883	74	38	.	.	PUNCT
fnp-5883	75	1	all	all	DET
fnp-5883	75	2	brain	brain	NOUN
fnp-5883	75	3	tissue	tissue	NOUN
fnp-5883	75	4	processing	processing	NOUN
fnp-5883	75	5	was	be	AUX
fnp-5883	75	6	performed	perform	VERB
fnp-5883	75	7	in	in	ADP
fnp-5883	75	8	the	the	DET
fnp-5883	75	9	neuropathology	neuropathology	NOUN
fnp-5883	75	10	brain	brain	PROPN
fnp-5883	75	11	bank	bank	PROPN
fnp-5883	75	12	&	&	CCONJ
fnp-5883	75	13	research	research	PROPN
fnp-5883	75	14	core	core	NOUN
fnp-5883	75	15	.	.	PUNCT
fnp-5883	76	1	the	the	DET
fnp-5883	76	2	brain	brain	NOUN
fnp-5883	76	3	donor	donor	NOUN
fnp-5883	76	4	was	be	AUX
fnp-5883	76	5	female	female	ADJ
fnp-5883	76	6	and	and	CCONJ
fnp-5883	76	7	was	be	AUX
fnp-5883	76	8	76	76	NUM
fnp-5883	76	9	years	year	NOUN
fnp-5883	76	10	old	old	ADJ
fnp-5883	76	11	at	at	ADP
fnp-5883	76	12	the	the	DET
fnp-5883	76	13	time	time	NOUN
fnp-5883	76	14	of	of	ADP
fnp-5883	76	15	death	death	NOUN
fnp-5883	76	16	.	.	PUNCT
fnp-5883	77	1	the	the	DET
fnp-5883	77	2	postmortem	postmortem	NOUN
fnp-5883	77	3	interval	interval	NOUN
fnp-5883	77	4	from	from	ADP
fnp-5883	77	5	time	time	NOUN
fnp-5883	77	6	of	of	ADP
fnp-5883	77	7	death	death	NOUN
fnp-5883	77	8	to	to	ADP
fnp-5883	77	9	freezing	freeze	VERB
fnp-5883	77	10	was	be	AUX
fnp-5883	77	11	70.5	70.5	NUM
fnp-5883	77	12	hours	hour	NOUN
fnp-5883	77	13	.	.	PUNCT
fnp-5883	78	1	the	the	DET
fnp-5883	78	2	brain	brain	NOUN
fnp-5883	78	3	was	be	AUX
fnp-5883	78	4	removed	remove	VERB
fnp-5883	78	5	from	from	ADP
fnp-5883	78	6	the	the	DET
fnp-5883	78	7	skull	skull	NOUN
fnp-5883	78	8	and	and	CCONJ
fnp-5883	78	9	placed	place	VERB
fnp-5883	78	10	in	in	ADP
fnp-5883	78	11	a	a	DET
fnp-5883	78	12	cooler	cooler	NOUN
fnp-5883	78	13	for	for	ADP
fnp-5883	78	14	transport	transport	NOUN
fnp-5883	78	15	from	from	ADP
fnp-5883	78	16	the	the	DET
fnp-5883	78	17	autopsy	autopsy	ADJ
fnp-5883	78	18	suite	suite	NOUN
fnp-5883	78	19	to	to	ADP
fnp-5883	78	20	the	the	DET
fnp-5883	78	21	brain	brain	NOUN
fnp-5883	78	22	bank	bank	NOUN
fnp-5883	78	23	.	.	PUNCT
fnp-5883	79	1	the	the	DET
fnp-5883	79	2	brain	brain	NOUN
fnp-5883	79	3	was	be	AUX
fnp-5883	79	4	bisected	bisect	VERB
fnp-5883	79	5	into	into	ADP
fnp-5883	79	6	two	two	NUM
fnp-5883	79	7	hemispheres	hemisphere	NOUN
fnp-5883	79	8	.	.	PUNCT
fnp-5883	80	1	the	the	DET
fnp-5883	80	2	right	right	ADJ
fnp-5883	80	3	hemisphere	hemisphere	ADV
fnp-5883	80	4	was	be	AUX
fnp-5883	80	5	immersion	immersion	NOUN
fnp-5883	80	6	fixed	fix	VERB
fnp-5883	80	7	in	in	ADP
fnp-5883	80	8	toto	toto	NOUN
fnp-5883	80	9	in	in	ADP
fnp-5883	80	10	10	10	NUM
fnp-5883	80	11	 	 	SPACE
fnp-5883	80	12	%	%	NOUN
fnp-5883	80	13	neutral	neutral	ADJ
fnp-5883	80	14	buffered	buffer	VERB
fnp-5883	80	15	formalin	formalin	NOUN
fnp-5883	80	16	,	,	PUNCT
fnp-5883	80	17	while	while	SCONJ
fnp-5883	80	18	the	the	DET
fnp-5883	80	19	left	leave	VERB
fnp-5883	80	20	hemisphere	hemisphere	ADV
fnp-5883	80	21	was	be	AUX
fnp-5883	80	22	sectioned	section	VERB
fnp-5883	80	23	fresh	fresh	ADV
fnp-5883	80	24	into	into	ADP
fnp-5883	80	25	approximately	approximately	ADV
fnp-5883	80	26	0.5	0.5	NUM
fnp-5883	80	27	 	 	SPACE
fnp-5883	80	28	cm	cm	NOUN
fnp-5883	80	29	coronal	coronal	ADJ
fnp-5883	80	30	slices	slice	NOUN
fnp-5883	80	31	.	.	PUNCT
fnp-5883	81	1	these	these	DET
fnp-5883	81	2	fresh	fresh	ADJ
fnp-5883	81	3	slices	slice	NOUN
fnp-5883	81	4	were	be	AUX
fnp-5883	81	5	parcellated	parcellate	VERB
fnp-5883	81	6	into	into	ADP
fnp-5883	81	7	approximately	approximately	ADV
fnp-5883	81	8	2	2	NUM
fnp-5883	81	9	 	 	SPACE
fnp-5883	81	10	x	x	NOUN
fnp-5883	81	11	 	 	SPACE
fnp-5883	81	12	2	2	NUM
fnp-5883	81	13	 	 	SPACE
fnp-5883	81	14	cm	cm	NOUN
fnp-5883	81	15	blocks	block	NOUN
fnp-5883	81	16	,	,	PUNCT
fnp-5883	81	17	and	and	CCONJ
fnp-5883	81	18	then	then	ADV
fnp-5883	81	19	cryopreserved	cryopreserve	VERB
fnp-5883	81	20	between	between	ADP
fnp-5883	81	21	teflon	teflon	NOUN
fnp-5883	81	22	-	-	PUNCT
fnp-5883	81	23	coated	coat	VERB
fnp-5883	81	24	metal	metal	NOUN
fnp-5883	81	25	plates	plate	NOUN
fnp-5883	81	26	cooled	cool	VERB
fnp-5883	81	27	at	at	ADP
fnp-5883	81	28	‑80	‑80	NOUN
fnp-5883	81	29	 	 	SPACE
fnp-5883	81	30	°	°	ADP
fnp-5883	81	31	c	c	NOUN
fnp-5883	81	32	.	.	PUNCT
fnp-5883	82	1	the	the	DET
fnp-5883	82	2	tissue	tissue	NOUN
fnp-5883	82	3	was	be	AUX
fnp-5883	82	4	stored	store	VERB
fnp-5883	82	5	at	at	ADP
fnp-5883	82	6	‑80	‑80	NOUN
fnp-5883	82	7	 	 	SPACE
fnp-5883	82	8	°	°	ADP
fnp-5883	82	9	c	c	NOUN
fnp-5883	82	10	for	for	ADP
fnp-5883	82	11	5.3	5.3	NUM
fnp-5883	82	12	years	year	NOUN
fnp-5883	82	13	.	.	PUNCT
fnp-5883	83	1	one	one	NUM
fnp-5883	83	2	frozen	frozen	ADJ
fnp-5883	83	3	tissue	tissue	NOUN
fnp-5883	83	4	block	block	NOUN
fnp-5883	83	5	of	of	ADP
fnp-5883	83	6	the	the	DET
fnp-5883	83	7	lateral	lateral	ADJ
fnp-5883	83	8	occipital	occipital	ADJ
fnp-5883	83	9	cortex	cortex	NOUN
fnp-5883	83	10	was	be	AUX
fnp-5883	83	11	then	then	ADV
fnp-5883	83	12	moved	move	VERB
fnp-5883	83	13	to	to	ADP
fnp-5883	83	14	a	a	DET
fnp-5883	83	15	‑20	‑20	ADJ
fnp-5883	83	16	 	 	SPACE
fnp-5883	83	17	°	°	ADP
fnp-5883	83	18	c	c	NOUN
fnp-5883	83	19	freezer	freezer	NOUN
fnp-5883	83	20	for	for	ADP
fnp-5883	83	21	6	6	NUM
fnp-5883	83	22	hours	hour	NOUN
fnp-5883	83	23	and	and	CCONJ
fnp-5883	83	24	10	10	NUM
fnp-5883	83	25	minutes	minute	NOUN
fnp-5883	83	26	.	.	PUNCT
fnp-5883	84	1	formalin	formalin	NOUN
fnp-5883	84	2	fixative	fixative	NOUN
fnp-5883	84	3	was	be	AUX
fnp-5883	84	4	also	also	ADV
fnp-5883	84	5	frozen	freeze	VERB
fnp-5883	84	6	at	at	ADP
fnp-5883	84	7	‑20	‑20	PRON
fnp-5883	84	8	 	 	SPACE
fnp-5883	84	9	°	°	NOUN
fnp-5883	84	10	c	c	NOUN
fnp-5883	84	11	and	and	CCONJ
fnp-5883	84	12	then	then	ADV
fnp-5883	84	13	moved	move	VERB
fnp-5883	84	14	to	to	ADP
fnp-5883	84	15	4	4	NUM
fnp-5883	84	16	 	 	SPACE
fnp-5883	84	17	°	°	NOUN
fnp-5883	84	18	c	c	NOUN
fnp-5883	84	19	at	at	ADP
fnp-5883	84	20	the	the	DET
fnp-5883	84	21	same	same	ADJ
fnp-5883	84	22	time	time	NOUN
fnp-5883	84	23	as	as	ADP
fnp-5883	84	24	the	the	DET
fnp-5883	84	25	tissue	tissue	NOUN
fnp-5883	84	26	.	.	PUNCT
fnp-5883	85	1	the	the	DET
fnp-5883	85	2	tissue	tissue	NOUN
fnp-5883	85	3	was	be	AUX
fnp-5883	85	4	placed	place	VERB
fnp-5883	85	5	in	in	ADP
fnp-5883	85	6	the	the	DET
fnp-5883	85	7	slushy	slushy	ADJ
fnp-5883	85	8	consistency	consistency	NOUN
fnp-5883	85	9	formalin	formalin	NOUN
fnp-5883	85	10	at	at	ADP
fnp-5883	85	11	4	4	NUM
fnp-5883	85	12	 	 	SPACE
fnp-5883	85	13	°	°	NOUN
fnp-5883	85	14	c	c	NOUN
fnp-5883	85	15	for	for	ADP
fnp-5883	85	16	18	18	NUM
fnp-5883	85	17	hours	hour	NOUN
fnp-5883	85	18	before	before	ADP
fnp-5883	85	19	being	be	AUX
fnp-5883	85	20	moved	move	VERB
fnp-5883	85	21	to	to	PART
fnp-5883	85	22	phosphate	phosphate	VERB
fnp-5883	85	23	-	-	PUNCT
fnp-5883	85	24	buffered	buffer	VERB
fnp-5883	85	25	saline	saline	NOUN
fnp-5883	85	26	(	(	PUNCT
fnp-5883	85	27	pbs	pbs	PROPN
fnp-5883	85	28	)	)	PUNCT
fnp-5883	85	29	containing	contain	VERB
fnp-5883	85	30	0.1	0.1	NUM
fnp-5883	85	31	 	 	SPACE
fnp-5883	85	32	%	%	NOUN
fnp-5883	85	33	sodium	sodium	NOUN
fnp-5883	85	34	azide	azide	NOUN
fnp-5883	85	35	at	at	ADP
fnp-5883	85	36	room	room	NOUN
fnp-5883	85	37	temperature	temperature	NOUN
fnp-5883	85	38	for	for	ADP
fnp-5883	85	39	4	4	NUM
fnp-5883	85	40	hours	hour	NOUN
fnp-5883	85	41	and	and	CCONJ
fnp-5883	85	42	45	45	NUM
fnp-5883	85	43	minutes	minute	NOUN
fnp-5883	85	44	.	.	PUNCT
fnp-5883	86	1	it	it	PRON
fnp-5883	86	2	was	be	AUX
fnp-5883	86	3	then	then	ADV
fnp-5883	86	4	placed	place	VERB
fnp-5883	86	5	in	in	ADP
fnp-5883	86	6	room	room	NOUN
fnp-5883	86	7	temperature	temperature	NOUN
fnp-5883	86	8	formalin	formalin	NOUN
fnp-5883	86	9	for	for	ADP
fnp-5883	86	10	55	55	NUM
fnp-5883	86	11	minutes	minute	NOUN
fnp-5883	86	12	before	before	ADP
fnp-5883	86	13	being	be	AUX
fnp-5883	86	14	processed	process	VERB
fnp-5883	86	15	for	for	ADP
fnp-5883	86	16	histology	histology	NOUN
fnp-5883	86	17	.	.	PUNCT
fnp-5883	87	1	frozen	freeze	VERB
fnp-5883	87	2	-	-	PUNCT
fnp-5883	87	3	thawed	thaw	VERB
fnp-5883	87	4	tissue	tissue	NOUN
fnp-5883	87	5	from	from	ADP
fnp-5883	87	6	the	the	DET
fnp-5883	87	7	lateral	lateral	ADJ
fnp-5883	87	8	occipital	occipital	ADJ
fnp-5883	87	9	cortex	cortex	NOUN
fnp-5883	87	10	region	region	NOUN
fnp-5883	87	11	was	be	AUX
fnp-5883	87	12	compared	compare	VERB
fnp-5883	87	13	to	to	PART
fnp-5883	87	14	immersion	immersion	VERB
fnp-5883	87	15	fixed	fix	VERB
fnp-5883	87	16	tissue	tissue	NOUN
fnp-5883	87	17	from	from	ADP
fnp-5883	87	18	the	the	DET
fnp-5883	87	19	calcarine	calcarine	NOUN
fnp-5883	87	20	cortex	cortex	NOUN
fnp-5883	87	21	of	of	ADP
fnp-5883	87	22	the	the	DET
fnp-5883	87	23	contralateral	contralateral	ADJ
fnp-5883	87	24	hemisphere	hemisphere	NOUN
fnp-5883	87	25	,	,	PUNCT
fnp-5883	87	26	which	which	PRON
fnp-5883	87	27	was	be	AUX
fnp-5883	87	28	fixed	fix	VERB
fnp-5883	87	29	in	in	ADP
fnp-5883	87	30	toto	toto	NOUN
fnp-5883	87	31	for	for	ADP
fnp-5883	87	32	37	37	NUM
fnp-5883	87	33	days	day	NOUN
fnp-5883	87	34	.	.	PUNCT
fnp-5883	88	1	the	the	DET
fnp-5883	88	2	fixed	fixed	ADJ
fnp-5883	88	3	tissue	tissue	NOUN
fnp-5883	88	4	was	be	AUX
fnp-5883	88	5	paraffin	paraffin	NOUN
fnp-5883	88	6	embedded	embed	VERB
fnp-5883	88	7	,	,	PUNCT
fnp-5883	88	8	and	and	CCONJ
fnp-5883	88	9	a	a	DET
fnp-5883	88	10	microtome	microtome	NOUN
fnp-5883	88	11	was	be	AUX
fnp-5883	88	12	used	use	VERB
fnp-5883	88	13	to	to	PART
fnp-5883	88	14	prepare	prepare	VERB
fnp-5883	88	15	consecutive	consecutive	ADJ
fnp-5883	88	16	sections	section	NOUN
fnp-5883	88	17	with	with	ADP
fnp-5883	88	18	a	a	DET
fnp-5883	88	19	thickness	thickness	NOUN
fnp-5883	88	20	of	of	ADP
fnp-5883	88	21	5	5	NUM
fnp-5883	88	22	 	 	SPACE
fnp-5883	88	23	µm	µm	NOUN
fnp-5883	88	24	,	,	PUNCT
fnp-5883	88	25	as	as	SCONJ
fnp-5883	88	26	previously	previously	ADV
fnp-5883	88	27	described	describe	VERB
fnp-5883	88	28	(	(	PUNCT
fnp-5883	88	29	mckenzie	mckenzie	PROPN
fnp-5883	88	30	et	et	PROPN
fnp-5883	88	31	al	al	PROPN
fnp-5883	88	32	.	.	PROPN
fnp-5883	88	33	,	,	PUNCT
fnp-5883	88	34	2022	2022	NUM
fnp-5883	88	35	)	)	PUNCT
fnp-5883	88	36	.	.	PUNCT
fnp-5883	89	1	these	these	DET
fnp-5883	89	2	sections	section	NOUN
fnp-5883	89	3	were	be	AUX
fnp-5883	89	4	then	then	ADV
fnp-5883	89	5	placed	place	VERB
fnp-5883	89	6	on	on	ADP
fnp-5883	89	7	glass	glass	NOUN
fnp-5883	89	8	slides	slide	NOUN
fnp-5883	89	9	,	,	PUNCT
fnp-5883	89	10	deparaffinized	deparaffinize	VERB
fnp-5883	89	11	,	,	PUNCT
fnp-5883	89	12	and	and	CCONJ
fnp-5883	89	13	stained	stain	VERB
fnp-5883	89	14	with	with	ADP
fnp-5883	89	15	hematoxylin	hematoxylin	NOUN
fnp-5883	89	16	and	and	CCONJ
fnp-5883	89	17	eosin	eosin	NOUN
fnp-5883	89	18	,	,	PUNCT
fnp-5883	89	19	followed	follow	VERB
fnp-5883	89	20	by	by	ADP
fnp-5883	89	21	imaging	image	VERB
fnp-5883	89	22	with	with	ADP
fnp-5883	89	23	a	a	DET
fnp-5883	89	24	digital	digital	ADJ
fnp-5883	89	25	slide	slide	NOUN
fnp-5883	89	26	scanner	scanner	NOUN
fnp-5883	89	27	.	.	PUNCT
fnp-5883	90	1	characteristics	characteristic	NOUN
fnp-5883	90	2	of	of	ADP
fnp-5883	90	3	included	include	VERB
fnp-5883	90	4	studies	study	NOUN
fnp-5883	90	5	through	through	ADP
fnp-5883	90	6	our	our	PRON
fnp-5883	90	7	formal	formal	ADJ
fnp-5883	90	8	search	search	NOUN
fnp-5883	90	9	procedure	procedure	NOUN
fnp-5883	90	10	,	,	PUNCT
fnp-5883	90	11	we	we	PRON
fnp-5883	90	12	screened	screen	VERB
fnp-5883	90	13	402	402	NUM
fnp-5883	90	14	abstracts	abstract	NOUN
fnp-5883	90	15	,	,	PUNCT
fnp-5883	90	16	assessed	assess	VERB
fnp-5883	90	17	108	108	NUM
fnp-5883	90	18	full	full	ADJ
fnp-5883	90	19	-	-	PUNCT
fnp-5883	90	20	text	text	NOUN
fnp-5883	90	21	articles	article	NOUN
fnp-5883	90	22	,	,	PUNCT
fnp-5883	90	23	and	and	CCONJ
fnp-5883	90	24	identified	identify	VERB
fnp-5883	90	25	50	50	NUM
fnp-5883	90	26	studies	study	NOUN
fnp-5883	90	27	meeting	meet	VERB
fnp-5883	90	28	our	our	PRON
fnp-5883	90	29	inclusion	inclusion	NOUN
fnp-5883	90	30	criteria	criterion	NOUN
fnp-5883	90	31	(	(	PUNCT
fnp-5883	90	32	figure	figure	NOUN
fnp-5883	90	33	 	 	SPACE
fnp-5883	90	34	1	1	NUM
fnp-5883	90	35	)	)	PUNCT
fnp-5883	90	36	.	.	PUNCT
fnp-5883	91	1	an	an	DET
fnp-5883	91	2	additional	additional	ADJ
fnp-5883	91	3	47	47	NUM
fnp-5883	91	4	studies	study	NOUN
fnp-5883	91	5	were	be	AUX
fnp-5883	91	6	included	include	VERB
fnp-5883	91	7	based	base	VERB
fnp-5883	91	8	on	on	ADP
fnp-5883	91	9	citation	citation	NOUN
fnp-5883	91	10	analysis	analysis	NOUN
fnp-5883	91	11	or	or	CCONJ
fnp-5883	91	12	supplementary	supplementary	ADJ
fnp-5883	91	13	ad	ad	NOUN
fnp-5883	91	14	hoc	hoc	X
fnp-5883	91	15	searches	search	NOUN
fnp-5883	91	16	,	,	PUNCT
fnp-5883	91	17	yielding	yield	VERB
fnp-5883	91	18	a	a	DET
fnp-5883	91	19	total	total	NOUN
fnp-5883	91	20	of	of	ADP
fnp-5883	91	21	97	97	NUM
fnp-5883	91	22	studies	study	NOUN
fnp-5883	91	23	for	for	ADP
fnp-5883	91	24	this	this	DET
fnp-5883	91	25	review	review	NOUN
fnp-5883	91	26	.	.	PUNCT
fnp-5883	92	1	we	we	PRON
fnp-5883	92	2	categorized	categorize	VERB
fnp-5883	92	3	the	the	DET
fnp-5883	92	4	included	include	VERB
fnp-5883	92	5	studies	study	NOUN
fnp-5883	92	6	into	into	ADP
fnp-5883	92	7	three	three	NUM
fnp-5883	92	8	partially	partially	ADV
fnp-5883	92	9	overlapping	overlap	VERB
fnp-5883	92	10	types	type	NOUN
fnp-5883	92	11	based	base	VERB
fnp-5883	92	12	on	on	ADP
fnp-5883	92	13	the	the	DET
fnp-5883	92	14	cryopreservation	cryopreservation	NOUN
fnp-5883	92	15	approach	approach	NOUN
fnp-5883	92	16	:	:	PUNCT
fnp-5883	92	17	(	(	PUNCT
fnp-5883	92	18	1	1	X
fnp-5883	92	19	)	)	PUNCT
fnp-5883	92	20	cryofixation	cryofixation	NOUN
fnp-5883	92	21	studies	study	NOUN
fnp-5883	92	22	,	,	PUNCT
fnp-5883	92	23	which	which	PRON
fnp-5883	92	24	used	use	VERB
fnp-5883	92	25	ultrarapid	ultrarapid	NOUN
fnp-5883	92	26	cooling	cool	VERB
fnp-5883	92	27	methods	method	NOUN
fnp-5883	92	28	like	like	ADP
fnp-5883	92	29	high	high	ADJ
fnp-5883	92	30	-	-	PUNCT
fnp-5883	92	31	pressure	pressure	NOUN
fnp-5883	92	32	freezing	freezing	NOUN
fnp-5883	92	33	or	or	CCONJ
fnp-5883	92	34	plunge	plunge	NOUN
fnp-5883	92	35	freezing	freezing	NOUN
fnp-5883	92	36	to	to	PART
fnp-5883	92	37	achieve	achieve	VERB
fnp-5883	92	38	vitrification	vitrification	NOUN
fnp-5883	92	39	of	of	ADP
fnp-5883	92	40	at	at	ADP
fnp-5883	92	41	least	least	ADJ
fnp-5883	92	42	part	part	NOUN
fnp-5883	92	43	of	of	ADP
fnp-5883	92	44	the	the	DET
fnp-5883	92	45	tissue	tissue	NOUN
fnp-5883	92	46	;	;	PUNCT
fnp-5883	92	47	(	(	PUNCT
fnp-5883	92	48	2	2	X
fnp-5883	92	49	)	)	PUNCT
fnp-5883	92	50	freezing	freeze	VERB
fnp-5883	92	51	studies	study	NOUN
fnp-5883	92	52	,	,	PUNCT
fnp-5883	92	53	which	which	PRON
fnp-5883	92	54	cooled	cool	VERB
fnp-5883	92	55	tissue	tissue	NOUN
fnp-5883	92	56	without	without	ADP
fnp-5883	92	57	cryoprotectants	cryoprotectant	NOUN
fnp-5883	92	58	at	at	ADP
fnp-5883	92	59	various	various	ADJ
fnp-5883	92	60	rates	rate	NOUN
fnp-5883	92	61	,	,	PUNCT
fnp-5883	92	62	allowing	allow	VERB
fnp-5883	92	63	some	some	DET
fnp-5883	92	64	degree	degree	NOUN
fnp-5883	92	65	of	of	ADP
fnp-5883	92	66	ice	ice	NOUN
fnp-5883	92	67	crystal	crystal	NOUN
fnp-5883	92	68	formation	formation	NOUN
fnp-5883	92	69	;	;	PUNCT
fnp-5883	92	70	and	and	CCONJ
fnp-5883	92	71	(	(	PUNCT
fnp-5883	92	72	3	3	X
fnp-5883	92	73	)	)	PUNCT
fnp-5883	92	74	cryopreservation	cryopreservation	NOUN
fnp-5883	92	75	with	with	ADP
fnp-5883	92	76	cryoprotectants	cryoprotectant	NOUN
fnp-5883	92	77	studies	study	NOUN
fnp-5883	92	78	,	,	PUNCT
fnp-5883	92	79	which	which	PRON
fnp-5883	92	80	used	use	VERB
fnp-5883	92	81	substances	substance	NOUN
fnp-5883	92	82	like	like	ADP
fnp-5883	92	83	dmso	dmso	NOUN
fnp-5883	92	84	or	or	CCONJ
fnp-5883	92	85	glycerol	glycerol	NOUN
fnp-5883	92	86	to	to	PART
fnp-5883	92	87	reduce	reduce	VERB
fnp-5883	92	88	ice	ice	NOUN
fnp-5883	92	89	crystal	crystal	NOUN
fnp-5883	92	90	formation	formation	NOUN
fnp-5883	92	91	during	during	ADP
fnp-5883	92	92	the	the	DET
fnp-5883	92	93	cooling	cool	VERB
fnp-5883	92	94	process	process	NOUN
fnp-5883	92	95	.	.	PUNCT
fnp-5883	93	1	the	the	DET
fnp-5883	93	2	studies	study	NOUN
fnp-5883	93	3	were	be	AUX
fnp-5883	93	4	performed	perform	VERB
fnp-5883	93	5	on	on	ADP
fnp-5883	93	6	tissue	tissue	NOUN
fnp-5883	93	7	from	from	ADP
fnp-5883	93	8	various	various	ADJ
fnp-5883	93	9	species	specie	NOUN
fnp-5883	93	10	,	,	PUNCT
fnp-5883	93	11	including	include	VERB
fnp-5883	93	12	humans	human	NOUN
fnp-5883	93	13	,	,	PUNCT
fnp-5883	93	14	rats	rat	NOUN
fnp-5883	93	15	,	,	PUNCT
fnp-5883	93	16	and	and	CCONJ
fnp-5883	93	17	mice	mouse	NOUN
fnp-5883	93	18	.	.	PUNCT
fnp-5883	94	1	before	before	ADP
fnp-5883	94	2	discussing	discuss	VERB
fnp-5883	94	3	the	the	DET
fnp-5883	94	4	outcomes	outcome	NOUN
fnp-5883	94	5	reported	report	VERB
fnp-5883	94	6	in	in	ADP
fnp-5883	94	7	these	these	DET
fnp-5883	94	8	studies	study	NOUN
fnp-5883	94	9	,	,	PUNCT
fnp-5883	94	10	we	we	PRON
fnp-5883	94	11	first	first	ADV
fnp-5883	94	12	review	review	VERB
fnp-5883	94	13	the	the	DET
fnp-5883	94	14	mechanistic	mechanistic	ADJ
fnp-5883	94	15	aspects	aspect	NOUN
fnp-5883	94	16	of	of	ADP
fnp-5883	94	17	cryopreservation	cryopreservation	NOUN
fnp-5883	94	18	that	that	PRON
fnp-5883	94	19	provide	provide	VERB
fnp-5883	94	20	important	important	ADJ
fnp-5883	94	21	context	context	NOUN
fnp-5883	94	22	for	for	ADP
fnp-5883	94	23	interpreting	interpret	VERB
fnp-5883	94	24	the	the	DET
fnp-5883	94	25	results	result	NOUN
fnp-5883	94	26	.	.	PUNCT
fnp-5883	95	1	figure	figure	VERB
fnp-5883	95	2	 	 	SPACE
fnp-5883	95	3	1	1	NUM
fnp-5883	95	4	.	.	PUNCT
fnp-5883	95	5	study	study	NOUN
fnp-5883	95	6	selection	selection	NOUN
fnp-5883	95	7	flow	flow	NOUN
fnp-5883	95	8	diagram	diagram	NOUN
fnp-5883	95	9	.	.	PUNCT
fnp-5883	96	1	studies	study	NOUN
fnp-5883	96	2	were	be	AUX
fnp-5883	96	3	screened	screen	VERB
fnp-5883	96	4	and	and	CCONJ
fnp-5883	96	5	selected	select	VERB
fnp-5883	96	6	using	use	VERB
fnp-5883	96	7	the	the	DET
fnp-5883	96	8	web	web	NOUN
fnp-5883	96	9	-	-	PUNCT
fnp-5883	96	10	based	base	VERB
fnp-5883	96	11	software	software	NOUN
fnp-5883	96	12	rayyan	rayyan	NOUN
fnp-5883	96	13	.	.	PUNCT
fnp-5883	97	1	mechanisms	mechanism	NOUN
fnp-5883	97	2	of	of	ADP
fnp-5883	97	3	brain	brain	NOUN
fnp-5883	97	4	cryopreservation	cryopreservation	NOUN
fnp-5883	97	5	physical	physical	ADJ
fnp-5883	97	6	aspects	aspect	NOUN
fnp-5883	97	7	of	of	ADP
fnp-5883	97	8	cooling	cool	VERB
fnp-5883	97	9	to	to	PART
fnp-5883	97	10	understand	understand	VERB
fnp-5883	97	11	the	the	DET
fnp-5883	97	12	effects	effect	NOUN
fnp-5883	97	13	of	of	ADP
fnp-5883	97	14	cryopreservation	cryopreservation	NOUN
fnp-5883	97	15	on	on	ADP
fnp-5883	97	16	brain	brain	NOUN
fnp-5883	97	17	cell	cell	NOUN
fnp-5883	97	18	structure	structure	NOUN
fnp-5883	97	19	,	,	PUNCT
fnp-5883	97	20	it	it	PRON
fnp-5883	97	21	is	be	AUX
fnp-5883	97	22	helpful	helpful	ADJ
fnp-5883	97	23	to	to	PART
fnp-5883	97	24	build	build	VERB
fnp-5883	97	25	up	up	ADP
fnp-5883	97	26	a	a	DET
fnp-5883	97	27	basic	basic	ADJ
fnp-5883	97	28	understanding	understanding	NOUN
fnp-5883	97	29	of	of	ADP
fnp-5883	97	30	what	what	PRON
fnp-5883	97	31	happens	happen	VERB
fnp-5883	97	32	during	during	ADP
fnp-5883	97	33	cryopreservation	cryopreservation	NOUN
fnp-5883	97	34	of	of	ADP
fnp-5883	97	35	brain	brain	NOUN
fnp-5883	97	36	tissue	tissue	NOUN
fnp-5883	97	37	(	(	PUNCT
fnp-5883	97	38	mcgrath	mcgrath	PROPN
fnp-5883	97	39	,	,	PUNCT
fnp-5883	97	40	1985	1985	NUM
fnp-5883	97	41	;	;	PUNCT
fnp-5883	97	42	karlsson	karlsson	PROPN
fnp-5883	97	43	and	and	CCONJ
fnp-5883	97	44	toner	toner	NOUN
fnp-5883	97	45	,	,	PUNCT
fnp-5883	97	46	1996	1996	NUM
fnp-5883	97	47	;	;	PUNCT
fnp-5883	97	48	wowk	wowk	PROPN
fnp-5883	97	49	,	,	PUNCT
fnp-5883	97	50	2010	2010	NUM
fnp-5883	97	51	)	)	PUNCT
fnp-5883	97	52	.	.	PUNCT
fnp-5883	98	1	note	note	VERB
fnp-5883	98	2	that	that	SCONJ
fnp-5883	98	3	this	this	DET
fnp-5883	98	4	section	section	NOUN
fnp-5883	98	5	is	be	AUX
fnp-5883	98	6	not	not	PART
fnp-5883	98	7	meant	mean	VERB
fnp-5883	98	8	to	to	PART
fnp-5883	98	9	be	be	AUX
fnp-5883	98	10	a	a	DET
fnp-5883	98	11	comprehensive	comprehensive	ADJ
fnp-5883	98	12	summary	summary	NOUN
fnp-5883	98	13	of	of	ADP
fnp-5883	98	14	this	this	DET
fnp-5883	98	15	complex	complex	ADJ
fnp-5883	98	16	topic	topic	NOUN
fnp-5883	98	17	,	,	PUNCT
fnp-5883	98	18	but	but	CCONJ
fnp-5883	98	19	rather	rather	ADV
fnp-5883	98	20	to	to	PART
fnp-5883	98	21	provide	provide	VERB
fnp-5883	98	22	a	a	DET
fnp-5883	98	23	context	context	NOUN
fnp-5883	98	24	for	for	ADP
fnp-5883	98	25	the	the	DET
fnp-5883	98	26	observed	observe	VERB
fnp-5883	98	27	phenomena	phenomenon	NOUN
fnp-5883	98	28	and	and	CCONJ
fnp-5883	98	29	outcomes	outcome	NOUN
fnp-5883	98	30	in	in	ADP
fnp-5883	98	31	the	the	DET
fnp-5883	98	32	empirical	empirical	ADJ
fnp-5883	98	33	studies	study	NOUN
fnp-5883	98	34	we	we	PRON
fnp-5883	98	35	reviewed	review	VERB
fnp-5883	98	36	.	.	PUNCT
fnp-5883	99	1	the	the	DET
fnp-5883	99	2	reason	reason	NOUN
fnp-5883	99	3	that	that	SCONJ
fnp-5883	99	4	cooling	cooling	NOUN
fnp-5883	99	5	can	can	AUX
fnp-5883	99	6	act	act	VERB
fnp-5883	99	7	as	as	ADP
fnp-5883	99	8	a	a	DET
fnp-5883	99	9	preservation	preservation	NOUN
fnp-5883	99	10	method	method	NOUN
fnp-5883	99	11	is	be	AUX
fnp-5883	99	12	that	that	SCONJ
fnp-5883	99	13	as	as	SCONJ
fnp-5883	99	14	temperature	temperature	NOUN
fnp-5883	99	15	decreases	decrease	VERB
fnp-5883	99	16	,	,	PUNCT
fnp-5883	99	17	the	the	DET
fnp-5883	99	18	kinetic	kinetic	ADJ
fnp-5883	99	19	energy	energy	NOUN
fnp-5883	99	20	of	of	ADP
fnp-5883	99	21	molecules	molecule	NOUN
fnp-5883	99	22	is	be	AUX
fnp-5883	99	23	reduced	reduce	VERB
fnp-5883	99	24	,	,	PUNCT
fnp-5883	99	25	leading	lead	VERB
fnp-5883	99	26	to	to	ADP
fnp-5883	99	27	slower	slow	ADJ
fnp-5883	99	28	molecular	molecular	ADJ
fnp-5883	99	29	motion	motion	NOUN
fnp-5883	99	30	and	and	CCONJ
fnp-5883	99	31	chemical	chemical	NOUN
fnp-5883	99	32	reactions	reaction	NOUN
fnp-5883	99	33	.	.	PUNCT
fnp-5883	100	1	at	at	ADP
fnp-5883	100	2	low	low	ADJ
fnp-5883	100	3	enough	enough	ADJ
fnp-5883	100	4	temperatures	temperature	NOUN
fnp-5883	100	5	,	,	PUNCT
fnp-5883	100	6	a	a	DET
fnp-5883	100	7	specimen	speciman	NOUN
fnp-5883	100	8	,	,	PUNCT
fnp-5883	100	9	or	or	CCONJ
fnp-5883	100	10	parts	part	NOUN
fnp-5883	100	11	of	of	ADP
fnp-5883	100	12	a	a	DET
fnp-5883	100	13	specimen	speciman	NOUN
fnp-5883	100	14	,	,	PUNCT
fnp-5883	100	15	will	will	AUX
fnp-5883	100	16	convert	convert	VERB
fnp-5883	100	17	from	from	ADP
fnp-5883	100	18	a	a	DET
fnp-5883	100	19	viscous	viscous	ADJ
fnp-5883	100	20	liquid	liquid	NOUN
fnp-5883	100	21	to	to	ADP
fnp-5883	100	22	a	a	DET
fnp-5883	100	23	glass	glass	NOUN
fnp-5883	100	24	-	-	PUNCT
fnp-5883	100	25	like	like	ADJ
fnp-5883	100	26	state	state	NOUN
fnp-5883	100	27	,	,	PUNCT
fnp-5883	100	28	in	in	ADP
fnp-5883	100	29	the	the	DET
fnp-5883	100	30	process	process	NOUN
fnp-5883	100	31	of	of	ADP
fnp-5883	100	32	vitrification	vitrification	NOUN
fnp-5883	100	33	(	(	PUNCT
fnp-5883	100	34	fahy	fahy	PROPN
fnp-5883	100	35	and	and	CCONJ
fnp-5883	100	36	wowk	wowk	NOUN
fnp-5883	100	37	,	,	PUNCT
fnp-5883	100	38	2021	2021	NUM
fnp-5883	100	39	)	)	PUNCT
fnp-5883	100	40	.	.	PUNCT
fnp-5883	101	1	this	this	PRON
fnp-5883	101	2	effectively	effectively	ADV
fnp-5883	101	3	“	"	PUNCT
fnp-5883	101	4	pauses	pause	VERB
fnp-5883	101	5	”	"	PUNCT
fnp-5883	101	6	biological	biological	ADJ
fnp-5883	101	7	processes	process	NOUN
fnp-5883	101	8	,	,	PUNCT
fnp-5883	101	9	including	include	VERB
fnp-5883	101	10	those	those	PRON
fnp-5883	101	11	that	that	PRON
fnp-5883	101	12	lead	lead	VERB
fnp-5883	101	13	to	to	ADP
fnp-5883	101	14	cell	cell	NOUN
fnp-5883	101	15	death	death	NOUN
fnp-5883	101	16	and	and	CCONJ
fnp-5883	101	17	tissue	tissue	NOUN
fnp-5883	101	18	decay	decay	NOUN
fnp-5883	101	19	(	(	PUNCT
fnp-5883	101	20	wowk	wowk	NOUN
fnp-5883	101	21	,	,	PUNCT
fnp-5883	101	22	2010	2010	NUM
fnp-5883	101	23	)	)	PUNCT
fnp-5883	101	24	.	.	PUNCT
fnp-5883	102	1	however	however	ADV
fnp-5883	102	2	,	,	PUNCT
fnp-5883	102	3	as	as	SCONJ
fnp-5883	102	4	the	the	DET
fnp-5883	102	5	temperature	temperature	NOUN
fnp-5883	102	6	decreases	decrease	VERB
fnp-5883	102	7	below	below	ADP
fnp-5883	102	8	the	the	DET
fnp-5883	102	9	freezing	freezing	ADJ
fnp-5883	102	10	point	point	NOUN
fnp-5883	102	11	of	of	ADP
fnp-5883	102	12	water	water	NOUN
fnp-5883	102	13	,	,	PUNCT
fnp-5883	102	14	water	water	NOUN
fnp-5883	102	15	molecules	molecule	NOUN
fnp-5883	102	16	also	also	ADV
fnp-5883	102	17	have	have	VERB
fnp-5883	102	18	a	a	DET
fnp-5883	102	19	thermodynamic	thermodynamic	ADJ
fnp-5883	102	20	tendency	tendency	NOUN
fnp-5883	102	21	to	to	PART
fnp-5883	102	22	spontaneously	spontaneously	ADV
fnp-5883	102	23	rearrange	rearrange	VERB
fnp-5883	102	24	into	into	ADP
fnp-5883	102	25	ice	ice	NOUN
fnp-5883	102	26	crystals	crystal	NOUN
fnp-5883	102	27	,	,	PUNCT
fnp-5883	102	28	which	which	PRON
fnp-5883	102	29	cause	cause	VERB
fnp-5883	102	30	damage	damage	NOUN
fnp-5883	102	31	to	to	ADP
fnp-5883	102	32	the	the	DET
fnp-5883	102	33	tissue	tissue	NOUN
fnp-5883	102	34	.	.	PUNCT
fnp-5883	103	1	in	in	ADP
fnp-5883	103	2	biological	biological	ADJ
fnp-5883	103	3	tissues	tissue	NOUN
fnp-5883	103	4	,	,	PUNCT
fnp-5883	103	5	there	there	PRON
fnp-5883	103	6	is	be	VERB
fnp-5883	103	7	a	a	DET
fnp-5883	103	8	further	further	ADJ
fnp-5883	103	9	complication	complication	NOUN
fnp-5883	103	10	,	,	PUNCT
fnp-5883	103	11	which	which	PRON
fnp-5883	103	12	is	be	AUX
fnp-5883	103	13	the	the	DET
fnp-5883	103	14	potential	potential	NOUN
fnp-5883	103	15	for	for	SCONJ
fnp-5883	103	16	non	non	ADJ
fnp-5883	103	17	-	-	ADJ
fnp-5883	103	18	water	water	ADJ
fnp-5883	103	19	substances	substance	NOUN
fnp-5883	103	20	to	to	PART
fnp-5883	103	21	crystallize	crystallize	VERB
fnp-5883	103	22	,	,	PUNCT
fnp-5883	103	23	such	such	ADJ
fnp-5883	103	24	as	as	ADP
fnp-5883	103	25	in	in	ADP
fnp-5883	103	26	the	the	DET
fnp-5883	103	27	process	process	NOUN
fnp-5883	103	28	of	of	ADP
fnp-5883	103	29	eutectic	eutectic	ADJ
fnp-5883	103	30	crystallization	crystallization	NOUN
fnp-5883	103	31	.	.	PUNCT
fnp-5883	104	1	we	we	PRON
fnp-5883	104	2	will	will	AUX
fnp-5883	104	3	discuss	discuss	VERB
fnp-5883	104	4	the	the	DET
fnp-5883	104	5	effects	effect	NOUN
fnp-5883	104	6	of	of	ADP
fnp-5883	104	7	ice	ice	NOUN
fnp-5883	104	8	formation	formation	NOUN
fnp-5883	104	9	first	first	ADV
fnp-5883	104	10	.	.	PUNCT
fnp-5883	105	1	how	how	SCONJ
fnp-5883	105	2	ice	ice	NOUN
fnp-5883	105	3	damages	damage	VERB
fnp-5883	105	4	biological	biological	ADJ
fnp-5883	105	5	tissue	tissue	NOUN
fnp-5883	105	6	ice	ice	NOUN
fnp-5883	105	7	formation	formation	NOUN
fnp-5883	105	8	leads	lead	VERB
fnp-5883	105	9	to	to	ADP
fnp-5883	105	10	damage	damage	NOUN
fnp-5883	105	11	to	to	ADP
fnp-5883	105	12	brain	brain	NOUN
fnp-5883	105	13	cell	cell	NOUN
fnp-5883	105	14	structure	structure	NOUN
fnp-5883	105	15	through	through	ADP
fnp-5883	105	16	several	several	ADJ
fnp-5883	105	17	mechanisms	mechanism	NOUN
fnp-5883	105	18	.	.	PUNCT
fnp-5883	106	1	these	these	DET
fnp-5883	106	2	forms	form	NOUN
fnp-5883	106	3	of	of	ADP
fnp-5883	106	4	damage	damage	NOUN
fnp-5883	106	5	can	can	AUX
fnp-5883	106	6	collectivity	collectivity	NOUN
fnp-5883	106	7	be	be	AUX
fnp-5883	106	8	called	call	VERB
fnp-5883	106	9	“	"	PUNCT
fnp-5883	106	10	freezing	freezing	ADJ
fnp-5883	106	11	injury	injury	NOUN
fnp-5883	106	12	”	"	PUNCT
fnp-5883	106	13	.	.	PUNCT
fnp-5883	107	1	first	first	ADV
fnp-5883	107	2	,	,	PUNCT
fnp-5883	107	3	formed	form	VERB
fnp-5883	107	4	ice	ice	NOUN
fnp-5883	107	5	crystals	crystal	NOUN
fnp-5883	107	6	can	can	AUX
fnp-5883	107	7	cause	cause	VERB
fnp-5883	107	8	mechanical	mechanical	ADJ
fnp-5883	107	9	damage	damage	NOUN
fnp-5883	107	10	to	to	ADP
fnp-5883	107	11	local	local	ADJ
fnp-5883	107	12	structures	structure	NOUN
fnp-5883	107	13	as	as	SCONJ
fnp-5883	107	14	they	they	PRON
fnp-5883	107	15	expand	expand	VERB
fnp-5883	107	16	,	,	PUNCT
fnp-5883	107	17	such	such	ADJ
fnp-5883	107	18	as	as	ADP
fnp-5883	107	19	causing	cause	VERB
fnp-5883	107	20	the	the	DET
fnp-5883	107	21	displacement	displacement	NOUN
fnp-5883	107	22	or	or	CCONJ
fnp-5883	107	23	rupture	rupture	NOUN
fnp-5883	107	24	of	of	ADP
fnp-5883	107	25	cell	cell	NOUN
fnp-5883	107	26	and	and	CCONJ
fnp-5883	107	27	organelle	organelle	NOUN
fnp-5883	107	28	membranes	membrane	NOUN
fnp-5883	107	29	(	(	PUNCT
fnp-5883	107	30	fahy	fahy	PROPN
fnp-5883	107	31	and	and	CCONJ
fnp-5883	107	32	wowk	wowk	NOUN
fnp-5883	107	33	,	,	PUNCT
fnp-5883	107	34	2021	2021	NUM
fnp-5883	107	35	)	)	PUNCT
fnp-5883	107	36	.	.	PUNCT
fnp-5883	108	1	second	second	ADJ
fnp-5883	108	2	,	,	PUNCT
fnp-5883	108	3	as	as	SCONJ
fnp-5883	108	4	water	water	NOUN
fnp-5883	108	5	is	be	AUX
fnp-5883	108	6	drawn	draw	VERB
fnp-5883	108	7	towards	towards	ADP
fnp-5883	108	8	growing	grow	VERB
fnp-5883	108	9	ice	ice	NOUN
fnp-5883	108	10	crystals	crystal	NOUN
fnp-5883	108	11	,	,	PUNCT
fnp-5883	108	12	this	this	PRON
fnp-5883	108	13	causes	cause	VERB
fnp-5883	108	14	large	large	ADJ
fnp-5883	108	15	stresses	stress	NOUN
fnp-5883	108	16	to	to	PART
fnp-5883	108	17	build	build	VERB
fnp-5883	108	18	up	up	ADP
fnp-5883	108	19	near	near	ADP
fnp-5883	108	20	the	the	DET
fnp-5883	108	21	ice	ice	NOUN
fnp-5883	108	22	-	-	PUNCT
fnp-5883	108	23	water	water	NOUN
fnp-5883	108	24	interface	interface	NOUN
fnp-5883	108	25	,	,	PUNCT
fnp-5883	108	26	which	which	PRON
fnp-5883	108	27	is	be	AUX
fnp-5883	108	28	a	a	DET
fnp-5883	108	29	phenomenon	phenomenon	NOUN
fnp-5883	108	30	known	know	VERB
fnp-5883	108	31	as	as	ADP
fnp-5883	108	32	cryosuction	cryosuction	NOUN
fnp-5883	108	33	(	(	PUNCT
fnp-5883	108	34	gerber	gerber	PROPN
fnp-5883	108	35	et	et	PROPN
fnp-5883	108	36	al	al	PROPN
fnp-5883	108	37	.	.	PROPN
fnp-5883	108	38	,	,	PUNCT
fnp-5883	108	39	2022	2022	NUM
fnp-5883	108	40	)	)	PUNCT
fnp-5883	108	41	.	.	PUNCT
fnp-5883	109	1	this	this	DET
fnp-5883	109	2	stress	stress	NOUN
fnp-5883	109	3	is	be	AUX
fnp-5883	109	4	associated	associate	VERB
fnp-5883	109	5	with	with	ADP
fnp-5883	109	6	high	high	ADJ
fnp-5883	109	7	local	local	ADJ
fnp-5883	109	8	pressures	pressure	NOUN
fnp-5883	109	9	of	of	ADP
fnp-5883	109	10	around	around	ADV
fnp-5883	109	11	1	1	NUM
fnp-5883	109	12	 	 	SPACE
fnp-5883	109	13	mpa	mpa	NOUN
fnp-5883	109	14	per	per	ADP
fnp-5883	109	15	degree	degree	NOUN
fnp-5883	109	16	of	of	ADP
fnp-5883	109	17	temperature	temperature	NOUN
fnp-5883	109	18	below	below	ADP
fnp-5883	109	19	the	the	DET
fnp-5883	109	20	freezing	freezing	NOUN
fnp-5883	109	21	point	point	NOUN
fnp-5883	109	22	,	,	PUNCT
fnp-5883	109	23	leading	lead	VERB
fnp-5883	109	24	to	to	ADP
fnp-5883	109	25	potential	potential	ADJ
fnp-5883	109	26	alterations	alteration	NOUN
fnp-5883	109	27	of	of	ADP
fnp-5883	109	28	biomolecular	biomolecular	ADJ
fnp-5883	109	29	distributions	distribution	NOUN
fnp-5883	109	30	in	in	ADP
fnp-5883	109	31	the	the	DET
fnp-5883	109	32	local	local	ADJ
fnp-5883	109	33	area	area	NOUN
fnp-5883	109	34	.	.	PUNCT
fnp-5883	110	1	third	third	ADJ
fnp-5883	110	2	,	,	PUNCT
fnp-5883	110	3	as	as	ADV
fnp-5883	110	4	extracellular	extracellular	ADJ
fnp-5883	110	5	and	and	CCONJ
fnp-5883	110	6	intracellular	intracellular	ADJ
fnp-5883	110	7	water	water	NOUN
fnp-5883	110	8	is	be	AUX
fnp-5883	110	9	removed	remove	VERB
fnp-5883	110	10	and	and	CCONJ
fnp-5883	110	11	cells	cell	NOUN
fnp-5883	110	12	become	become	AUX
fnp-5883	110	13	dehydrated	dehydrated	ADJ
fnp-5883	110	14	,	,	PUNCT
fnp-5883	110	15	high	high	ADJ
fnp-5883	110	16	solute	solute	NOUN
fnp-5883	110	17	concentrations	concentration	NOUN
fnp-5883	110	18	in	in	ADP
fnp-5883	110	19	the	the	DET
fnp-5883	110	20	cells	cell	NOUN
fnp-5883	110	21	lead	lead	VERB
fnp-5883	110	22	to	to	ADP
fnp-5883	110	23	structural	structural	ADJ
fnp-5883	110	24	damage	damage	NOUN
fnp-5883	110	25	(	(	PUNCT
fnp-5883	110	26	fahy	fahy	PROPN
fnp-5883	110	27	and	and	CCONJ
fnp-5883	110	28	wowk	wowk	PROPN
fnp-5883	110	29	,	,	PUNCT
fnp-5883	110	30	2015	2015	NUM
fnp-5883	110	31	)	)	PUNCT
fnp-5883	110	32	.	.	PUNCT
fnp-5883	111	1	damage	damage	NOUN
fnp-5883	111	2	due	due	ADP
fnp-5883	111	3	to	to	ADP
fnp-5883	111	4	high	high	ADJ
fnp-5883	111	5	solute	solute	NOUN
fnp-5883	111	6	concentrations	concentration	NOUN
fnp-5883	111	7	has	have	AUX
fnp-5883	111	8	been	be	AUX
fnp-5883	111	9	called	call	VERB
fnp-5883	111	10	“	"	PUNCT
fnp-5883	111	11	solution	solution	NOUN
fnp-5883	111	12	effects	effect	NOUN
fnp-5883	111	13	”	"	PUNCT
fnp-5883	111	14	(	(	PUNCT
fnp-5883	111	15	mazur	mazur	PROPN
fnp-5883	111	16	et	et	PROPN
fnp-5883	111	17	al	al	PROPN
fnp-5883	111	18	.	.	PROPN
fnp-5883	111	19	,	,	PUNCT
fnp-5883	111	20	1972	1972	NUM
fnp-5883	111	21	)	)	PUNCT
fnp-5883	111	22	.	.	PUNCT
fnp-5883	112	1	for	for	ADP
fnp-5883	112	2	example	example	NOUN
fnp-5883	112	3	,	,	PUNCT
fnp-5883	112	4	solution	solution	NOUN
fnp-5883	112	5	effects	effect	NOUN
fnp-5883	112	6	have	have	AUX
fnp-5883	112	7	been	be	AUX
fnp-5883	112	8	reported	report	VERB
fnp-5883	112	9	to	to	PART
fnp-5883	112	10	cause	cause	VERB
fnp-5883	112	11	the	the	DET
fnp-5883	112	12	denaturation	denaturation	NOUN
fnp-5883	112	13	and	and	CCONJ
fnp-5883	112	14	aggregation	aggregation	NOUN
fnp-5883	112	15	of	of	ADP
fnp-5883	112	16	biomolecules	biomolecule	NOUN
fnp-5883	112	17	(	(	PUNCT
fnp-5883	112	18	li	li	PROPN
fnp-5883	112	19	et	et	PROPN
fnp-5883	112	20	al	al	PROPN
fnp-5883	112	21	.	.	PROPN
fnp-5883	112	22	,	,	PUNCT
fnp-5883	112	23	2023	2023	NUM
fnp-5883	112	24	)	)	PUNCT
fnp-5883	112	25	.	.	PUNCT
fnp-5883	113	1	the	the	DET
fnp-5883	113	2	formation	formation	NOUN
fnp-5883	113	3	of	of	ADP
fnp-5883	113	4	ice	ice	NOUN
fnp-5883	113	5	during	during	ADP
fnp-5883	113	6	freezing	freezing	NOUN
fnp-5883	113	7	can	can	AUX
fnp-5883	113	8	be	be	AUX
fnp-5883	113	9	used	use	VERB
fnp-5883	113	10	to	to	PART
fnp-5883	113	11	permeabilize	permeabilize	VERB
fnp-5883	113	12	cells	cell	NOUN
fnp-5883	113	13	,	,	PUNCT
fnp-5883	113	14	which	which	PRON
fnp-5883	113	15	has	have	AUX
fnp-5883	113	16	been	be	AUX
fnp-5883	113	17	attributed	attribute	VERB
fnp-5883	113	18	to	to	ADP
fnp-5883	113	19	both	both	CCONJ
fnp-5883	113	20	mechanical	mechanical	ADJ
fnp-5883	113	21	disruption	disruption	NOUN
fnp-5883	113	22	of	of	ADP
fnp-5883	113	23	membranes	membrane	NOUN
fnp-5883	113	24	by	by	ADP
fnp-5883	113	25	ice	ice	NOUN
fnp-5883	113	26	and	and	CCONJ
fnp-5883	113	27	osmotic	osmotic	ADJ
fnp-5883	113	28	forces	force	NOUN
fnp-5883	113	29	(	(	PUNCT
fnp-5883	113	30	zhang	zhang	X
fnp-5883	113	31	et	et	PROPN
fnp-5883	113	32	al	al	PROPN
fnp-5883	113	33	.	.	PROPN
fnp-5883	113	34	,	,	PUNCT
fnp-5883	113	35	2016	2016	NUM
fnp-5883	113	36	)	)	PUNCT
fnp-5883	113	37	.	.	PUNCT
fnp-5883	114	1	smaller	small	ADJ
fnp-5883	114	2	ice	ice	NOUN
fnp-5883	114	3	crystals	crystal	NOUN
fnp-5883	114	4	may	may	AUX
fnp-5883	114	5	be	be	AUX
fnp-5883	114	6	particularly	particularly	ADV
fnp-5883	114	7	likely	likely	ADJ
fnp-5883	114	8	to	to	PART
fnp-5883	114	9	cause	cause	VERB
fnp-5883	114	10	the	the	DET
fnp-5883	114	11	membrane	membrane	NOUN
fnp-5883	114	12	damage	damage	NOUN
fnp-5883	114	13	that	that	PRON
fnp-5883	114	14	contributes	contribute	VERB
fnp-5883	114	15	to	to	ADP
fnp-5883	114	16	cell	cell	NOUN
fnp-5883	114	17	permeabilization	permeabilization	NOUN
fnp-5883	114	18	(	(	PUNCT
fnp-5883	114	19	zhang	zhang	X
fnp-5883	114	20	et	et	PROPN
fnp-5883	114	21	al	al	PROPN
fnp-5883	114	22	.	.	PROPN
fnp-5883	114	23	,	,	PUNCT
fnp-5883	114	24	2016	2016	NUM
fnp-5883	114	25	)	)	PUNCT
fnp-5883	114	26	.	.	PUNCT
fnp-5883	115	1	as	as	SCONJ
fnp-5883	115	2	cell	cell	NOUN
fnp-5883	115	3	membranes	membrane	NOUN
fnp-5883	115	4	are	be	AUX
fnp-5883	115	5	permeabilized	permeabilize	VERB
fnp-5883	115	6	,	,	PUNCT
fnp-5883	115	7	this	this	PRON
fnp-5883	115	8	allows	allow	VERB
fnp-5883	115	9	soluble	soluble	ADJ
fnp-5883	115	10	biomolecules	biomolecule	NOUN
fnp-5883	115	11	to	to	PART
fnp-5883	115	12	leak	leak	VERB
fnp-5883	115	13	out	out	ADP
fnp-5883	115	14	of	of	ADP
fnp-5883	115	15	cells	cell	NOUN
fnp-5883	115	16	and	and	CCONJ
fnp-5883	115	17	potentially	potentially	ADV
fnp-5883	115	18	diffuse	diffuse	VERB
fnp-5883	115	19	away	away	ADV
fnp-5883	115	20	from	from	ADP
fnp-5883	115	21	their	their	PRON
fnp-5883	115	22	original	original	ADJ
fnp-5883	115	23	locations	location	NOUN
fnp-5883	115	24	,	,	PUNCT
fnp-5883	115	25	which	which	PRON
fnp-5883	115	26	is	be	AUX
fnp-5883	115	27	another	another	DET
fnp-5883	115	28	form	form	NOUN
fnp-5883	115	29	of	of	ADP
fnp-5883	115	30	damage	damage	NOUN
fnp-5883	115	31	to	to	ADP
fnp-5883	115	32	brain	brain	NOUN
fnp-5883	115	33	cell	cell	NOUN
fnp-5883	115	34	structure	structure	NOUN
fnp-5883	115	35	during	during	ADP
fnp-5883	115	36	cryopreservation	cryopreservation	NOUN
fnp-5883	115	37	(	(	PUNCT
fnp-5883	115	38	rossner	rossner	NOUN
fnp-5883	115	39	et	et	PROPN
fnp-5883	115	40	al	al	PROPN
fnp-5883	115	41	.	.	PROPN
fnp-5883	115	42	,	,	PUNCT
fnp-5883	115	43	2006	2006	NUM
fnp-5883	115	44	;	;	PUNCT
fnp-5883	115	45	notter	notter	PROPN
fnp-5883	115	46	et	et	PROPN
fnp-5883	115	47	al	al	PROPN
fnp-5883	115	48	.	.	PROPN
fnp-5883	115	49	,	,	PUNCT
fnp-5883	115	50	2014	2014	NUM
fnp-5883	115	51	;	;	PUNCT
fnp-5883	115	52	gellhaar	gellhaar	NOUN
fnp-5883	115	53	et	et	NOUN
fnp-5883	115	54	al	al	PROPN
fnp-5883	115	55	.	.	PROPN
fnp-5883	115	56	,	,	PUNCT
fnp-5883	115	57	2017	2017	NUM
fnp-5883	115	58	)	)	PUNCT
fnp-5883	115	59	.	.	PUNCT
fnp-5883	116	1	the	the	DET
fnp-5883	116	2	location	location	NOUN
fnp-5883	116	3	of	of	ADP
fnp-5883	116	4	ice	ice	NOUN
fnp-5883	116	5	formation	formation	NOUN
fnp-5883	116	6	across	across	ADP
fnp-5883	116	7	tissues	tissue	NOUN
fnp-5883	116	8	is	be	AUX
fnp-5883	116	9	not	not	PART
fnp-5883	116	10	random	random	ADJ
fnp-5883	116	11	,	,	PUNCT
fnp-5883	116	12	but	but	CCONJ
fnp-5883	116	13	rather	rather	ADV
fnp-5883	116	14	can	can	AUX
fnp-5883	116	15	be	be	AUX
fnp-5883	116	16	predicted	predict	VERB
fnp-5883	116	17	to	to	ADP
fnp-5883	116	18	some	some	DET
fnp-5883	116	19	degree	degree	NOUN
fnp-5883	116	20	.	.	PUNCT
fnp-5883	117	1	in	in	ADP
fnp-5883	117	2	many	many	ADJ
fnp-5883	117	3	organs	organ	NOUN
fnp-5883	117	4	,	,	PUNCT
fnp-5883	117	5	ice	ice	NOUN
fnp-5883	117	6	formation	formation	NOUN
fnp-5883	117	7	tends	tend	VERB
fnp-5883	117	8	to	to	PART
fnp-5883	117	9	propagate	propagate	VERB
fnp-5883	117	10	along	along	ADP
fnp-5883	117	11	the	the	DET
fnp-5883	117	12	vascular	vascular	ADJ
fnp-5883	117	13	system	system	NOUN
fnp-5883	117	14	,	,	PUNCT
fnp-5883	117	15	likely	likely	ADJ
fnp-5883	117	16	because	because	SCONJ
fnp-5883	117	17	there	there	PRON
fnp-5883	117	18	is	be	VERB
fnp-5883	117	19	no	no	DET
fnp-5883	117	20	barrier	barrier	NOUN
fnp-5883	117	21	to	to	ADP
fnp-5883	117	22	crystal	crystal	NOUN
fnp-5883	117	23	growth	growth	NOUN
fnp-5883	117	24	in	in	ADP
fnp-5883	117	25	these	these	DET
fnp-5883	117	26	areas	area	NOUN
fnp-5883	117	27	(	(	PUNCT
fnp-5883	117	28	rubinsky	rubinsky	NOUN
fnp-5883	117	29	and	and	CCONJ
fnp-5883	117	30	pegg	pegg	NOUN
fnp-5883	117	31	,	,	PUNCT
fnp-5883	117	32	1988	1988	NUM
fnp-5883	117	33	)	)	PUNCT
fnp-5883	117	34	.	.	PUNCT
fnp-5883	118	1	when	when	SCONJ
fnp-5883	118	2	these	these	DET
fnp-5883	118	3	tissues	tissue	NOUN
fnp-5883	118	4	are	be	AUX
fnp-5883	118	5	examined	examine	VERB
fnp-5883	118	6	under	under	ADP
fnp-5883	118	7	a	a	DET
fnp-5883	118	8	microscope	microscope	NOUN
fnp-5883	118	9	,	,	PUNCT
fnp-5883	118	10	the	the	DET
fnp-5883	118	11	structural	structural	ADJ
fnp-5883	118	12	changes	change	NOUN
fnp-5883	118	13	caused	cause	VERB
fnp-5883	118	14	by	by	ADP
fnp-5883	118	15	large	large	ADJ
fnp-5883	118	16	ice	ice	NOUN
fnp-5883	118	17	crystal	crystal	NOUN
fnp-5883	118	18	formation	formation	NOUN
fnp-5883	118	19	also	also	ADV
fnp-5883	118	20	follow	follow	VERB
fnp-5883	118	21	predictable	predictable	ADJ
fnp-5883	118	22	patterns	pattern	NOUN
fnp-5883	118	23	.	.	PUNCT
fnp-5883	119	1	upon	upon	SCONJ
fnp-5883	119	2	rewarming	rewarming	NOUN
fnp-5883	119	3	,	,	PUNCT
fnp-5883	119	4	areas	area	NOUN
fnp-5883	119	5	of	of	ADP
fnp-5883	119	6	tissue	tissue	NOUN
fnp-5883	119	7	that	that	PRON
fnp-5883	119	8	previously	previously	ADV
fnp-5883	119	9	underwent	undergo	VERB
fnp-5883	119	10	ice	ice	NOUN
fnp-5883	119	11	formation	formation	NOUN
fnp-5883	119	12	tend	tend	VERB
fnp-5883	119	13	to	to	PART
fnp-5883	119	14	have	have	VERB
fnp-5883	119	15	anisotropic	anisotropic	NOUN
fnp-5883	119	16	spaces	space	NOUN
fnp-5883	119	17	empty	empty	ADJ
fnp-5883	119	18	of	of	ADP
fnp-5883	119	19	tissue	tissue	NOUN
fnp-5883	119	20	,	,	PUNCT
fnp-5883	119	21	indicative	indicative	ADJ
fnp-5883	119	22	of	of	ADP
fnp-5883	119	23	the	the	DET
fnp-5883	119	24	crystalline	crystalline	ADJ
fnp-5883	119	25	structures	structure	NOUN
fnp-5883	119	26	of	of	ADP
fnp-5883	119	27	ice	ice	NOUN
fnp-5883	119	28	when	when	SCONJ
fnp-5883	119	29	visualized	visualize	VERB
fnp-5883	119	30	under	under	ADP
fnp-5883	119	31	a	a	DET
fnp-5883	119	32	microscope	microscope	NOUN
fnp-5883	119	33	.	.	PUNCT
fnp-5883	120	1	these	these	DET
fnp-5883	120	2	ice	ice	NOUN
fnp-5883	120	3	damage	damage	NOUN
fnp-5883	120	4	artifacts	artifact	NOUN
fnp-5883	120	5	have	have	AUX
fnp-5883	120	6	been	be	AUX
fnp-5883	120	7	described	describe	VERB
fnp-5883	120	8	as	as	ADP
fnp-5883	120	9	vacuoles	vacuole	NOUN
fnp-5883	120	10	,	,	PUNCT
fnp-5883	120	11	“	"	PUNCT
fnp-5883	120	12	swiss	swiss	ADJ
fnp-5883	120	13	cheese	cheese	NOUN
fnp-5883	120	14	holes	hole	NOUN
fnp-5883	120	15	,	,	PUNCT
fnp-5883	120	16	”	"	PUNCT
fnp-5883	120	17	or	or	CCONJ
fnp-5883	120	18	as	as	ADP
fnp-5883	120	19	having	have	VERB
fnp-5883	120	20	a	a	DET
fnp-5883	120	21	“	"	PUNCT
fnp-5883	120	22	sponge	sponge	NOUN
fnp-5883	120	23	-	-	PUNCT
fnp-5883	120	24	like	like	ADJ
fnp-5883	120	25	”	"	PUNCT
fnp-5883	120	26	appearance	appearance	NOUN
fnp-5883	120	27	(	(	PUNCT
fnp-5883	120	28	chambers	chamber	NOUN
fnp-5883	120	29	et	et	NOUN
fnp-5883	120	30	al	al	PROPN
fnp-5883	120	31	.	.	PROPN
fnp-5883	120	32	,	,	PUNCT
fnp-5883	120	33	1932	1932	NUM
fnp-5883	120	34	;	;	PUNCT
fnp-5883	120	35	taqi	taqi	PROPN
fnp-5883	120	36	et	et	PROPN
fnp-5883	120	37	al	al	PROPN
fnp-5883	120	38	.	.	PROPN
fnp-5883	120	39	,	,	PUNCT
fnp-5883	120	40	2018	2018	NUM
fnp-5883	120	41	)	)	PUNCT
fnp-5883	120	42	.	.	PUNCT
fnp-5883	121	1	in	in	ADP
fnp-5883	121	2	addition	addition	NOUN
fnp-5883	121	3	to	to	ADP
fnp-5883	121	4	generating	generate	VERB
fnp-5883	121	5	open	open	ADJ
fnp-5883	121	6	spaces	space	NOUN
fnp-5883	121	7	,	,	PUNCT
fnp-5883	121	8	ice	ice	NOUN
fnp-5883	121	9	formation	formation	NOUN
fnp-5883	121	10	can	can	AUX
fnp-5883	121	11	cause	cause	VERB
fnp-5883	121	12	other	other	ADJ
fnp-5883	121	13	structural	structural	ADJ
fnp-5883	121	14	changes	change	NOUN
fnp-5883	121	15	,	,	PUNCT
fnp-5883	121	16	such	such	ADJ
fnp-5883	121	17	as	as	ADP
fnp-5883	121	18	disrupting	disrupt	VERB
fnp-5883	121	19	membrane	membrane	NOUN
fnp-5883	121	20	structures	structure	NOUN
fnp-5883	121	21	or	or	CCONJ
fnp-5883	121	22	creating	create	VERB
fnp-5883	121	23	a	a	DET
fnp-5883	121	24	web	web	NOUN
fnp-5883	121	25	-	-	ADJ
fnp-5883	121	26	like	like	ADJ
fnp-5883	121	27	pattern	pattern	NOUN
fnp-5883	121	28	(	(	PUNCT
fnp-5883	121	29	reticulation	reticulation	NOUN
fnp-5883	121	30	)	)	PUNCT
fnp-5883	121	31	in	in	ADP
fnp-5883	121	32	the	the	DET
fnp-5883	121	33	nucleus	nucleus	NOUN
fnp-5883	121	34	and	and	CCONJ
fnp-5883	121	35	cytoplasm	cytoplasm	ADJ
fnp-5883	121	36	(	(	PUNCT
fnp-5883	121	37	möbius	möbius	PROPN
fnp-5883	121	38	et	et	PROPN
fnp-5883	121	39	al	al	PROPN
fnp-5883	121	40	.	.	PROPN
fnp-5883	121	41	,	,	PUNCT
fnp-5883	121	42	2010	2010	NUM
fnp-5883	121	43	)	)	PUNCT
fnp-5883	121	44	.	.	PUNCT
fnp-5883	122	1	this	this	DET
fnp-5883	122	2	reticulation	reticulation	NOUN
fnp-5883	122	3	can	can	AUX
fnp-5883	122	4	look	look	VERB
fnp-5883	122	5	like	like	ADP
fnp-5883	122	6	irregularly	irregularly	ADV
fnp-5883	122	7	shaped	shape	VERB
fnp-5883	122	8	dark	dark	ADJ
fnp-5883	122	9	strands	strand	NOUN
fnp-5883	122	10	when	when	SCONJ
fnp-5883	122	11	viewed	view	VERB
fnp-5883	122	12	under	under	ADP
fnp-5883	122	13	an	an	DET
fnp-5883	122	14	electron	electron	NOUN
fnp-5883	122	15	microscope	microscope	NOUN
fnp-5883	122	16	.	.	PUNCT
fnp-5883	123	1	after	after	ADP
fnp-5883	123	2	severe	severe	ADJ
fnp-5883	123	3	ice	ice	NOUN
fnp-5883	123	4	damage	damage	NOUN
fnp-5883	123	5	,	,	PUNCT
fnp-5883	123	6	the	the	DET
fnp-5883	123	7	tissue	tissue	NOUN
fnp-5883	123	8	can	can	AUX
fnp-5883	123	9	be	be	AUX
fnp-5883	123	10	dramatically	dramatically	ADV
fnp-5883	123	11	modified	modify	VERB
fnp-5883	123	12	.	.	PUNCT
fnp-5883	124	1	it	it	PRON
fnp-5883	124	2	has	have	AUX
fnp-5883	124	3	been	be	AUX
fnp-5883	124	4	reported	report	VERB
fnp-5883	124	5	that	that	SCONJ
fnp-5883	124	6	large	large	ADJ
fnp-5883	124	7	ice	ice	NOUN
fnp-5883	124	8	crystals	crystal	NOUN
fnp-5883	124	9	can	can	AUX
fnp-5883	124	10	distort	distort	VERB
fnp-5883	124	11	cell	cell	NOUN
fnp-5883	124	12	membranes	membrane	NOUN
fnp-5883	124	13	and	and	CCONJ
fnp-5883	124	14	organelles	organelle	NOUN
fnp-5883	124	15	to	to	ADP
fnp-5883	124	16	such	such	DET
fnp-5883	124	17	an	an	DET
fnp-5883	124	18	extent	extent	NOUN
fnp-5883	124	19	that	that	SCONJ
fnp-5883	124	20	the	the	DET
fnp-5883	124	21	pre	pre	ADJ
fnp-5883	124	22	-	-	ADJ
fnp-5883	124	23	existing	exist	VERB
fnp-5883	124	24	structural	structural	ADJ
fnp-5883	124	25	configuration	configuration	NOUN
fnp-5883	124	26	is	be	AUX
fnp-5883	124	27	unrecognizable	unrecognizable	ADJ
fnp-5883	124	28	and	and	CCONJ
fnp-5883	124	29	the	the	DET
fnp-5883	124	30	underlying	underlie	VERB
fnp-5883	124	31	structure	structure	NOUN
fnp-5883	124	32	of	of	ADP
fnp-5883	124	33	the	the	DET
fnp-5883	124	34	specimen	speciman	NOUN
fnp-5883	124	35	appears	appear	VERB
fnp-5883	124	36	to	to	PART
fnp-5883	124	37	be	be	AUX
fnp-5883	124	38	destroyed	destroy	VERB
fnp-5883	124	39	(	(	PUNCT
fnp-5883	124	40	ryan	ryan	PROPN
fnp-5883	124	41	,	,	PUNCT
fnp-5883	124	42	1991	1991	NUM
fnp-5883	124	43	)	)	PUNCT
fnp-5883	124	44	.	.	PUNCT
fnp-5883	125	1	how	how	SCONJ
fnp-5883	125	2	ice	ice	NOUN
fnp-5883	125	3	damage	damage	NOUN
fnp-5883	125	4	can	can	AUX
fnp-5883	125	5	be	be	AUX
fnp-5883	125	6	prevented	prevent	VERB
fnp-5883	125	7	one	one	NUM
fnp-5883	125	8	method	method	NOUN
fnp-5883	125	9	available	available	ADJ
fnp-5883	125	10	to	to	PART
fnp-5883	125	11	entirely	entirely	ADV
fnp-5883	125	12	prevent	prevent	VERB
fnp-5883	125	13	ice	ice	NOUN
fnp-5883	125	14	formation	formation	NOUN
fnp-5883	125	15	is	be	AUX
fnp-5883	125	16	the	the	DET
fnp-5883	125	17	use	use	NOUN
fnp-5883	125	18	of	of	ADP
fnp-5883	125	19	an	an	DET
fnp-5883	125	20	extremely	extremely	ADV
fnp-5883	125	21	rapid	rapid	ADJ
fnp-5883	125	22	cooling	cool	VERB
fnp-5883	125	23	rate	rate	NOUN
fnp-5883	125	24	.	.	PUNCT
fnp-5883	126	1	if	if	SCONJ
fnp-5883	126	2	the	the	DET
fnp-5883	126	3	temperature	temperature	NOUN
fnp-5883	126	4	of	of	ADP
fnp-5883	126	5	the	the	DET
fnp-5883	126	6	biospecimen	biospeciman	NOUN
fnp-5883	126	7	is	be	AUX
fnp-5883	126	8	reduced	reduce	VERB
fnp-5883	126	9	very	very	ADV
fnp-5883	126	10	quickly	quickly	ADV
fnp-5883	126	11	,	,	PUNCT
fnp-5883	126	12	ice	ice	NOUN
fnp-5883	126	13	does	do	AUX
fnp-5883	126	14	not	not	PART
fnp-5883	126	15	have	have	VERB
fnp-5883	126	16	the	the	DET
fnp-5883	126	17	time	time	NOUN
fnp-5883	126	18	to	to	PART
fnp-5883	126	19	form	form	VERB
fnp-5883	126	20	or	or	CCONJ
fnp-5883	126	21	grow	grow	VERB
fnp-5883	126	22	to	to	ADP
fnp-5883	126	23	a	a	DET
fnp-5883	126	24	meaningful	meaningful	ADJ
fnp-5883	126	25	degree	degree	NOUN
fnp-5883	126	26	.	.	PUNCT
fnp-5883	127	1	the	the	DET
fnp-5883	127	2	minimum	minimum	ADJ
fnp-5883	127	3	rate	rate	NOUN
fnp-5883	127	4	of	of	ADP
fnp-5883	127	5	cooling	cool	VERB
fnp-5883	127	6	necessary	necessary	ADJ
fnp-5883	127	7	to	to	PART
fnp-5883	127	8	cool	cool	VERB
fnp-5883	127	9	a	a	DET
fnp-5883	127	10	sample	sample	NOUN
fnp-5883	127	11	fast	fast	ADV
fnp-5883	127	12	enough	enough	ADV
fnp-5883	127	13	to	to	PART
fnp-5883	127	14	“	"	PUNCT
fnp-5883	127	15	outrun	outrun	VERB
fnp-5883	127	16	”	"	PUNCT
fnp-5883	127	17	the	the	DET
fnp-5883	127	18	speed	speed	NOUN
fnp-5883	127	19	of	of	ADP
fnp-5883	127	20	ice	ice	NOUN
fnp-5883	127	21	formation	formation	NOUN
fnp-5883	127	22	and	and	CCONJ
fnp-5883	127	23	achieve	achieve	VERB
fnp-5883	127	24	vitrification	vitrification	NOUN
fnp-5883	127	25	is	be	AUX
fnp-5883	127	26	called	call	VERB
fnp-5883	127	27	the	the	DET
fnp-5883	127	28	critical	critical	ADJ
fnp-5883	127	29	cooling	cool	VERB
fnp-5883	127	30	rate	rate	NOUN
fnp-5883	127	31	(	(	PUNCT
fnp-5883	127	32	fahy	fahy	PROPN
fnp-5883	127	33	and	and	CCONJ
fnp-5883	127	34	wowk	wowk	NOUN
fnp-5883	127	35	,	,	PUNCT
fnp-5883	127	36	2021	2021	NUM
fnp-5883	127	37	)	)	PUNCT
fnp-5883	127	38	.	.	PUNCT
fnp-5883	128	1	because	because	SCONJ
fnp-5883	128	2	the	the	DET
fnp-5883	128	3	required	required	ADJ
fnp-5883	128	4	cooling	cool	VERB
fnp-5883	128	5	rate	rate	NOUN
fnp-5883	128	6	is	be	AUX
fnp-5883	128	7	very	very	ADV
fnp-5883	128	8	rapid	rapid	ADJ
fnp-5883	128	9	,	,	PUNCT
fnp-5883	128	10	this	this	PRON
fnp-5883	128	11	generally	generally	ADV
fnp-5883	128	12	requires	require	VERB
fnp-5883	128	13	exposing	expose	VERB
fnp-5883	128	14	very	very	ADV
fnp-5883	128	15	small	small	ADJ
fnp-5883	128	16	samples	sample	NOUN
fnp-5883	128	17	of	of	ADP
fnp-5883	128	18	cells	cell	NOUN
fnp-5883	128	19	or	or	CCONJ
fnp-5883	128	20	tissues	tissue	NOUN
fnp-5883	128	21	to	to	ADP
fnp-5883	128	22	extremely	extremely	ADV
fnp-5883	128	23	cold	cold	ADJ
fnp-5883	128	24	temperatures	temperature	NOUN
fnp-5883	128	25	.	.	PUNCT
fnp-5883	129	1	in	in	ADP
fnp-5883	129	2	larger	large	ADJ
fnp-5883	129	3	samples	sample	NOUN
fnp-5883	129	4	,	,	PUNCT
fnp-5883	129	5	it	it	PRON
fnp-5883	129	6	is	be	AUX
fnp-5883	129	7	not	not	PART
fnp-5883	129	8	possible	possible	ADJ
fnp-5883	129	9	to	to	PART
fnp-5883	129	10	perform	perform	VERB
fnp-5883	129	11	this	this	PRON
fnp-5883	129	12	,	,	PUNCT
fnp-5883	129	13	because	because	SCONJ
fnp-5883	129	14	the	the	DET
fnp-5883	129	15	slow	slow	ADJ
fnp-5883	129	16	rate	rate	NOUN
fnp-5883	129	17	of	of	ADP
fnp-5883	129	18	dissipation	dissipation	NOUN
fnp-5883	129	19	of	of	ADP
fnp-5883	129	20	heat	heat	NOUN
fnp-5883	129	21	by	by	ADP
fnp-5883	129	22	convection	convection	NOUN
fnp-5883	129	23	in	in	ADP
fnp-5883	129	24	biological	biological	ADJ
fnp-5883	129	25	tissue	tissue	NOUN
fnp-5883	129	26	prevents	prevent	VERB
fnp-5883	129	27	it	it	PRON
fnp-5883	129	28	.	.	PUNCT
fnp-5883	130	1	for	for	ADP
fnp-5883	130	2	example	example	NOUN
fnp-5883	130	3	,	,	PUNCT
fnp-5883	130	4	one	one	NUM
fnp-5883	130	5	source	source	NOUN
fnp-5883	130	6	reports	report	VERB
fnp-5883	130	7	that	that	SCONJ
fnp-5883	130	8	an	an	DET
fnp-5883	130	9	infinitely	infinitely	ADV
fnp-5883	130	10	fast	fast	ADJ
fnp-5883	130	11	cooling	cool	VERB
fnp-5883	130	12	rate	rate	NOUN
fnp-5883	130	13	at	at	ADP
fnp-5883	130	14	the	the	DET
fnp-5883	130	15	surface	surface	NOUN
fnp-5883	130	16	of	of	ADP
fnp-5883	130	17	an	an	DET
fnp-5883	130	18	aqueous	aqueous	ADJ
fnp-5883	130	19	sample	sample	NOUN
fnp-5883	130	20	would	would	AUX
fnp-5883	130	21	lead	lead	VERB
fnp-5883	130	22	to	to	ADP
fnp-5883	130	23	a	a	DET
fnp-5883	130	24	cooling	cool	VERB
fnp-5883	130	25	rate	rate	NOUN
fnp-5883	130	26	of	of	ADP
fnp-5883	130	27	around	around	ADP
fnp-5883	130	28	5000	5000	NUM
fnp-5883	130	29	 	 	SPACE
fnp-5883	130	30	k	k	X
fnp-5883	130	31	/	/	SYM
fnp-5883	130	32	s	s	X
fnp-5883	130	33	at	at	ADP
fnp-5883	130	34	the	the	DET
fnp-5883	130	35	depth	depth	NOUN
fnp-5883	130	36	of	of	ADP
fnp-5883	130	37	100	100	NUM
fnp-5883	130	38	 	 	SPACE
fnp-5883	130	39	µm	µm	NOUN
fnp-5883	130	40	,	,	PUNCT
fnp-5883	130	41	which	which	PRON
fnp-5883	130	42	is	be	AUX
fnp-5883	130	43	not	not	PART
fnp-5883	130	44	sufficiently	sufficiently	ADV
fnp-5883	130	45	fast	fast	ADJ
fnp-5883	130	46	for	for	ADP
fnp-5883	130	47	vitrification	vitrification	NOUN
fnp-5883	130	48	(	(	PUNCT
fnp-5883	130	49	studer	studer	NOUN
fnp-5883	130	50	et	et	NOUN
fnp-5883	130	51	al	al	PROPN
fnp-5883	130	52	.	.	PROPN
fnp-5883	130	53	,	,	PUNCT
fnp-5883	130	54	2014	2014	NUM
fnp-5883	130	55	)	)	PUNCT
fnp-5883	130	56	.	.	PUNCT
fnp-5883	131	1	another	another	DET
fnp-5883	131	2	method	method	NOUN
fnp-5883	131	3	to	to	PART
fnp-5883	131	4	prevent	prevent	VERB
fnp-5883	131	5	ice	ice	NOUN
fnp-5883	131	6	formation	formation	NOUN
fnp-5883	131	7	is	be	AUX
fnp-5883	131	8	to	to	PART
fnp-5883	131	9	add	add	VERB
fnp-5883	131	10	cryoprotectants	cryoprotectant	NOUN
fnp-5883	131	11	to	to	ADP
fnp-5883	131	12	the	the	DET
fnp-5883	131	13	tissue	tissue	NOUN
fnp-5883	131	14	.	.	PUNCT
fnp-5883	132	1	cryoprotectants	cryoprotectant	NOUN
fnp-5883	132	2	are	be	AUX
fnp-5883	132	3	chemicals	chemical	NOUN
fnp-5883	132	4	that	that	PRON
fnp-5883	132	5	decrease	decrease	VERB
fnp-5883	132	6	or	or	CCONJ
fnp-5883	132	7	prevent	prevent	VERB
fnp-5883	132	8	freezing	freeze	VERB
fnp-5883	132	9	injury	injury	NOUN
fnp-5883	132	10	during	during	ADP
fnp-5883	132	11	cryopreservation	cryopreservation	NOUN
fnp-5883	132	12	(	(	PUNCT
fnp-5883	132	13	fahy	fahy	PROPN
fnp-5883	132	14	and	and	CCONJ
fnp-5883	132	15	wowk	wowk	PROPN
fnp-5883	132	16	,	,	PUNCT
fnp-5883	132	17	2015	2015	NUM
fnp-5883	132	18	)	)	PUNCT
fnp-5883	132	19	.	.	PUNCT
fnp-5883	133	1	cryoprotectants	cryoprotectant	NOUN
fnp-5883	133	2	can	can	AUX
fnp-5883	133	3	aid	aid	VERB
fnp-5883	133	4	in	in	ADP
fnp-5883	133	5	vitrification	vitrification	NOUN
fnp-5883	133	6	by	by	ADP
fnp-5883	133	7	directly	directly	ADV
fnp-5883	133	8	forming	form	VERB
fnp-5883	133	9	hydrogen	hydrogen	NOUN
fnp-5883	133	10	bonds	bond	NOUN
fnp-5883	133	11	with	with	ADP
fnp-5883	133	12	water	water	NOUN
fnp-5883	133	13	molecules	molecule	NOUN
fnp-5883	133	14	(	(	PUNCT
fnp-5883	133	15	lee	lee	PROPN
fnp-5883	133	16	and	and	CCONJ
fnp-5883	133	17	baiz	baiz	ADJ
fnp-5883	133	18	,	,	PUNCT
fnp-5883	133	19	2022	2022	NUM
fnp-5883	133	20	)	)	PUNCT
fnp-5883	133	21	,	,	PUNCT
fnp-5883	133	22	causing	cause	VERB
fnp-5883	133	23	freezing	freezing	NOUN
fnp-5883	133	24	point	point	NOUN
fnp-5883	133	25	depression	depression	NOUN
fnp-5883	133	26	as	as	ADP
fnp-5883	133	27	a	a	DET
fnp-5883	133	28	colligative	colligative	ADJ
fnp-5883	133	29	effect	effect	NOUN
fnp-5883	133	30	and/or	and/or	CCONJ
fnp-5883	133	31	increasing	increase	VERB
fnp-5883	133	32	the	the	DET
fnp-5883	133	33	viscosity	viscosity	NOUN
fnp-5883	133	34	of	of	ADP
fnp-5883	133	35	the	the	DET
fnp-5883	133	36	tissue	tissue	NOUN
fnp-5883	133	37	.	.	PUNCT
fnp-5883	134	1	although	although	SCONJ
fnp-5883	134	2	there	there	PRON
fnp-5883	134	3	are	be	VERB
fnp-5883	134	4	endogenous	endogenous	ADJ
fnp-5883	134	5	chemicals	chemical	NOUN
fnp-5883	134	6	in	in	ADP
fnp-5883	134	7	tissues	tissue	NOUN
fnp-5883	134	8	that	that	PRON
fnp-5883	134	9	act	act	VERB
fnp-5883	134	10	as	as	ADP
fnp-5883	134	11	cryoprotectants	cryoprotectant	NOUN
fnp-5883	134	12	,	,	PUNCT
fnp-5883	134	13	the	the	DET
fnp-5883	134	14	term	term	NOUN
fnp-5883	134	15	“	"	PUNCT
fnp-5883	134	16	cryoprotectant	cryoprotectant	NOUN
fnp-5883	134	17	”	"	PUNCT
fnp-5883	134	18	usually	usually	ADV
fnp-5883	134	19	refers	refer	VERB
fnp-5883	134	20	to	to	ADP
fnp-5883	134	21	an	an	DET
fnp-5883	134	22	external	external	ADJ
fnp-5883	134	23	chemical	chemical	NOUN
fnp-5883	134	24	that	that	PRON
fnp-5883	134	25	is	be	AUX
fnp-5883	134	26	added	add	VERB
fnp-5883	134	27	to	to	ADP
fnp-5883	134	28	a	a	DET
fnp-5883	134	29	tissue	tissue	NOUN
fnp-5883	134	30	for	for	ADP
fnp-5883	134	31	this	this	DET
fnp-5883	134	32	purpose	purpose	NOUN
fnp-5883	134	33	.	.	PUNCT
fnp-5883	135	1	these	these	DET
fnp-5883	135	2	substances	substance	NOUN
fnp-5883	135	3	often	often	ADV
fnp-5883	135	4	replace	replace	VERB
fnp-5883	135	5	water	water	NOUN
fnp-5883	135	6	as	as	ADP
fnp-5883	135	7	the	the	DET
fnp-5883	135	8	tissue	tissue	NOUN
fnp-5883	135	9	solvent	solvent	NOUN
fnp-5883	135	10	.	.	PUNCT
fnp-5883	136	1	examples	example	NOUN
fnp-5883	136	2	of	of	ADP
fnp-5883	136	3	cryoprotectants	cryoprotectant	NOUN
fnp-5883	136	4	include	include	VERB
fnp-5883	136	5	small	small	ADJ
fnp-5883	136	6	molecules	molecule	NOUN
fnp-5883	136	7	such	such	ADJ
fnp-5883	136	8	as	as	ADP
fnp-5883	136	9	glycerol	glycerol	NOUN
fnp-5883	136	10	,	,	PUNCT
fnp-5883	136	11	ethylene	ethylene	NOUN
fnp-5883	136	12	glycol	glycol	NOUN
fnp-5883	136	13	,	,	PUNCT
fnp-5883	136	14	dimethyl	dimethyl	NOUN
fnp-5883	136	15	sulfoxide	sulfoxide	NOUN
fnp-5883	136	16	(	(	PUNCT
fnp-5883	136	17	dmso	dmso	NOUN
fnp-5883	136	18	)	)	PUNCT
fnp-5883	136	19	,	,	PUNCT
fnp-5883	136	20	or	or	CCONJ
fnp-5883	136	21	ice	ice	NOUN
fnp-5883	136	22	blockers	blocker	NOUN
fnp-5883	136	23	such	such	ADJ
fnp-5883	136	24	as	as	ADP
fnp-5883	136	25	antifreeze	antifreeze	ADJ
fnp-5883	136	26	proteins	protein	NOUN
fnp-5883	136	27	(	(	PUNCT
fnp-5883	136	28	fahy	fahy	PROPN
fnp-5883	136	29	and	and	CCONJ
fnp-5883	136	30	wowk	wowk	PROPN
fnp-5883	136	31	,	,	PUNCT
fnp-5883	136	32	2015	2015	NUM
fnp-5883	136	33	)	)	PUNCT
fnp-5883	136	34	.	.	PUNCT
fnp-5883	137	1	notably	notably	ADV
fnp-5883	137	2	,	,	PUNCT
fnp-5883	137	3	cryoprotectants	cryoprotectant	NOUN
fnp-5883	137	4	and	and	CCONJ
fnp-5883	137	5	faster	fast	ADV
fnp-5883	137	6	cooling	cool	VERB
fnp-5883	137	7	rates	rate	NOUN
fnp-5883	137	8	can	can	AUX
fnp-5883	137	9	work	work	VERB
fnp-5883	137	10	together	together	ADV
fnp-5883	137	11	to	to	PART
fnp-5883	137	12	protect	protect	VERB
fnp-5883	137	13	a	a	DET
fnp-5883	137	14	tissue	tissue	NOUN
fnp-5883	137	15	from	from	ADP
fnp-5883	137	16	ice	ice	NOUN
fnp-5883	137	17	damage	damage	NOUN
fnp-5883	137	18	during	during	ADP
fnp-5883	137	19	cryopreservation	cryopreservation	NOUN
fnp-5883	137	20	.	.	PUNCT
fnp-5883	138	1	with	with	ADP
fnp-5883	138	2	a	a	DET
fnp-5883	138	3	higher	high	ADJ
fnp-5883	138	4	concentration	concentration	NOUN
fnp-5883	138	5	of	of	ADP
fnp-5883	138	6	cryoprotectants	cryoprotectant	NOUN
fnp-5883	138	7	,	,	PUNCT
fnp-5883	138	8	a	a	DET
fnp-5883	138	9	lower	low	ADJ
fnp-5883	138	10	cooling	cool	VERB
fnp-5883	138	11	rate	rate	NOUN
fnp-5883	138	12	is	be	AUX
fnp-5883	138	13	necessary	necessary	ADJ
fnp-5883	138	14	to	to	PART
fnp-5883	138	15	prevent	prevent	VERB
fnp-5883	138	16	ice	ice	NOUN
fnp-5883	138	17	damage	damage	NOUN
fnp-5883	138	18	,	,	PUNCT
fnp-5883	138	19	and	and	CCONJ
fnp-5883	138	20	vice	vice	ADV
fnp-5883	138	21	versa	versa	ADV
fnp-5883	138	22	(	(	PUNCT
fnp-5883	138	23	wowk	wowk	PROPN
fnp-5883	138	24	,	,	PUNCT
fnp-5883	138	25	2010	2010	NUM
fnp-5883	138	26	)	)	PUNCT
fnp-5883	138	27	.	.	PUNCT
fnp-5883	139	1	by	by	ADP
fnp-5883	139	2	preventing	prevent	VERB
fnp-5883	139	3	ice	ice	NOUN
fnp-5883	139	4	formation	formation	NOUN
fnp-5883	139	5	,	,	PUNCT
fnp-5883	139	6	both	both	CCONJ
fnp-5883	139	7	cryoprotectants	cryoprotectant	NOUN
fnp-5883	139	8	and	and	CCONJ
fnp-5883	139	9	faster	fast	ADV
fnp-5883	139	10	cooling	cool	VERB
fnp-5883	139	11	rates	rate	NOUN
fnp-5883	139	12	are	be	AUX
fnp-5883	139	13	expected	expect	VERB
fnp-5883	139	14	to	to	PART
fnp-5883	139	15	improve	improve	VERB
fnp-5883	139	16	structural	structural	ADJ
fnp-5883	139	17	preservation	preservation	NOUN
fnp-5883	139	18	quality	quality	NOUN
fnp-5883	139	19	during	during	ADP
fnp-5883	139	20	cryopreservation	cryopreservation	NOUN
fnp-5883	139	21	.	.	PUNCT
fnp-5883	140	1	however	however	ADV
fnp-5883	140	2	,	,	PUNCT
fnp-5883	140	3	the	the	DET
fnp-5883	140	4	introduction	introduction	NOUN
fnp-5883	140	5	of	of	ADP
fnp-5883	140	6	cryoprotectants	cryoprotectant	NOUN
fnp-5883	140	7	can	can	AUX
fnp-5883	140	8	also	also	ADV
fnp-5883	140	9	alter	alter	VERB
fnp-5883	140	10	cellular	cellular	ADJ
fnp-5883	140	11	and	and	CCONJ
fnp-5883	140	12	molecular	molecular	ADJ
fnp-5883	140	13	structures	structure	NOUN
fnp-5883	140	14	(	(	PUNCT
fnp-5883	140	15	robards	robard	NOUN
fnp-5883	140	16	and	and	CCONJ
fnp-5883	140	17	sleytr	sleytr	NOUN
fnp-5883	140	18	,	,	PUNCT
fnp-5883	140	19	1985	1985	NUM
fnp-5883	140	20	,	,	PUNCT
fnp-5883	140	21	p.	p.	NOUN
fnp-5883	140	22	 	 	SPACE
fnp-5883	140	23	77	77	NUM
fnp-5883	140	24	)	)	PUNCT
fnp-5883	140	25	.	.	PUNCT
fnp-5883	141	1	most	most	ADV
fnp-5883	141	2	commonly	commonly	ADV
fnp-5883	141	3	,	,	PUNCT
fnp-5883	141	4	the	the	DET
fnp-5883	141	5	high	high	ADJ
fnp-5883	141	6	concentration	concentration	NOUN
fnp-5883	141	7	of	of	ADP
fnp-5883	141	8	cryoprotectants	cryoprotectant	NOUN
fnp-5883	141	9	needed	need	VERB
fnp-5883	141	10	to	to	PART
fnp-5883	141	11	prevent	prevent	VERB
fnp-5883	141	12	ice	ice	NOUN
fnp-5883	141	13	formation	formation	NOUN
fnp-5883	141	14	leads	lead	VERB
fnp-5883	141	15	to	to	ADP
fnp-5883	141	16	cell	cell	NOUN
fnp-5883	141	17	shrinkage	shrinkage	NOUN
fnp-5883	141	18	and/or	and/or	CCONJ
fnp-5883	141	19	osmotic	osmotic	ADJ
fnp-5883	141	20	stress	stress	NOUN
fnp-5883	141	21	,	,	PUNCT
fnp-5883	141	22	particularly	particularly	ADV
fnp-5883	141	23	if	if	SCONJ
fnp-5883	141	24	the	the	DET
fnp-5883	141	25	addition	addition	NOUN
fnp-5883	141	26	of	of	ADP
fnp-5883	141	27	cryoprotectants	cryoprotectant	NOUN
fnp-5883	141	28	is	be	AUX
fnp-5883	141	29	not	not	PART
fnp-5883	141	30	performed	perform	VERB
fnp-5883	141	31	in	in	ADP
fnp-5883	141	32	a	a	DET
fnp-5883	141	33	graded	grade	VERB
fnp-5883	141	34	manner	manner	NOUN
fnp-5883	141	35	.	.	PUNCT
fnp-5883	142	1	to	to	PART
fnp-5883	142	2	minimize	minimize	VERB
fnp-5883	142	3	damage	damage	NOUN
fnp-5883	142	4	from	from	ADP
fnp-5883	142	5	osmotic	osmotic	ADJ
fnp-5883	142	6	stress	stress	NOUN
fnp-5883	142	7	,	,	PUNCT
fnp-5883	142	8	cryoprotectants	cryoprotectant	NOUN
fnp-5883	142	9	are	be	AUX
fnp-5883	142	10	typically	typically	ADV
fnp-5883	142	11	added	add	VERB
fnp-5883	142	12	slowly	slowly	ADV
fnp-5883	142	13	,	,	PUNCT
fnp-5883	142	14	allowing	allow	VERB
fnp-5883	142	15	cells	cell	NOUN
fnp-5883	142	16	time	time	NOUN
fnp-5883	142	17	to	to	PART
fnp-5883	142	18	equilibrate	equilibrate	VERB
fnp-5883	142	19	and	and	CCONJ
fnp-5883	142	20	adjust	adjust	VERB
fnp-5883	142	21	to	to	ADP
fnp-5883	142	22	the	the	DET
fnp-5883	142	23	changing	change	VERB
fnp-5883	142	24	osmotic	osmotic	ADJ
fnp-5883	142	25	environment	environment	NOUN
fnp-5883	142	26	.	.	PUNCT
fnp-5883	143	1	in	in	ADP
fnp-5883	143	2	larger	large	ADJ
fnp-5883	143	3	samples	sample	NOUN
fnp-5883	143	4	,	,	PUNCT
fnp-5883	143	5	the	the	DET
fnp-5883	143	6	slow	slow	ADJ
fnp-5883	143	7	addition	addition	NOUN
fnp-5883	143	8	of	of	ADP
fnp-5883	143	9	cryoprotectants	cryoprotectant	NOUN
fnp-5883	143	10	via	via	ADP
fnp-5883	143	11	immersion	immersion	NOUN
fnp-5883	143	12	also	also	ADV
fnp-5883	143	13	renders	render	VERB
fnp-5883	143	14	the	the	DET
fnp-5883	143	15	tissue	tissue	NOUN
fnp-5883	143	16	vulnerable	vulnerable	ADJ
fnp-5883	143	17	to	to	ADP
fnp-5883	143	18	decomposition	decomposition	NOUN
fnp-5883	143	19	during	during	ADP
fnp-5883	143	20	this	this	DET
fnp-5883	143	21	process	process	NOUN
fnp-5883	143	22	.	.	PUNCT
fnp-5883	144	1	one	one	NUM
fnp-5883	144	2	way	way	NOUN
fnp-5883	144	3	to	to	PART
fnp-5883	144	4	minimize	minimize	VERB
fnp-5883	144	5	the	the	DET
fnp-5883	144	6	time	time	NOUN
fnp-5883	144	7	of	of	ADP
fnp-5883	144	8	cellular	cellular	ADJ
fnp-5883	144	9	decomposition	decomposition	NOUN
fnp-5883	144	10	is	be	AUX
fnp-5883	144	11	to	to	PART
fnp-5883	144	12	perfuse	perfuse	VERB
fnp-5883	144	13	cryoprotectants	cryoprotectant	NOUN
fnp-5883	144	14	through	through	ADP
fnp-5883	144	15	the	the	DET
fnp-5883	144	16	blood	blood	NOUN
fnp-5883	144	17	vessels	vessel	NOUN
fnp-5883	144	18	of	of	ADP
fnp-5883	144	19	the	the	DET
fnp-5883	144	20	brain	brain	NOUN
fnp-5883	144	21	,	,	PUNCT
fnp-5883	144	22	which	which	PRON
fnp-5883	144	23	allows	allow	VERB
fnp-5883	144	24	the	the	DET
fnp-5883	144	25	cryoprotectants	cryoprotectant	NOUN
fnp-5883	144	26	to	to	PART
fnp-5883	144	27	extravasate	extravasate	VERB
fnp-5883	144	28	into	into	ADP
fnp-5883	144	29	the	the	DET
fnp-5883	144	30	tissue	tissue	NOUN
fnp-5883	144	31	.	.	PUNCT
fnp-5883	145	1	however	however	ADV
fnp-5883	145	2	,	,	PUNCT
fnp-5883	145	3	perfusion	perfusion	NOUN
fnp-5883	145	4	of	of	ADP
fnp-5883	145	5	the	the	DET
fnp-5883	145	6	postmortem	postmortem	NOUN
fnp-5883	145	7	brain	brain	NOUN
fnp-5883	145	8	is	be	AUX
fnp-5883	145	9	a	a	DET
fnp-5883	145	10	challenging	challenging	ADJ
fnp-5883	145	11	problem	problem	NOUN
fnp-5883	145	12	,	,	PUNCT
fnp-5883	145	13	especially	especially	ADV
fnp-5883	145	14	in	in	ADP
fnp-5883	145	15	the	the	DET
fnp-5883	145	16	setting	setting	NOUN
fnp-5883	145	17	of	of	ADP
fnp-5883	145	18	extended	extended	ADJ
fnp-5883	145	19	periods	period	NOUN
fnp-5883	145	20	without	without	ADP
fnp-5883	145	21	blood	blood	NOUN
fnp-5883	145	22	flow	flow	NOUN
fnp-5883	145	23	(	(	PUNCT
fnp-5883	145	24	mcfadden	mcfadden	PROPN
fnp-5883	145	25	et	et	PROPN
fnp-5883	145	26	al	al	PROPN
fnp-5883	145	27	.	.	PROPN
fnp-5883	145	28	,	,	PUNCT
fnp-5883	145	29	2019	2019	NUM
fnp-5883	145	30	)	)	PUNCT
fnp-5883	145	31	.	.	PUNCT
fnp-5883	146	1	ice	ice	NOUN
fnp-5883	146	2	formation	formation	NOUN
fnp-5883	146	3	during	during	ADP
fnp-5883	146	4	freezing	freeze	VERB
fnp-5883	146	5	procedures	procedure	NOUN
fnp-5883	146	6	in	in	ADP
fnp-5883	146	7	the	the	DET
fnp-5883	146	8	absence	absence	NOUN
fnp-5883	146	9	of	of	ADP
fnp-5883	146	10	cryoprotectants	cryoprotectant	NOUN
fnp-5883	146	11	,	,	PUNCT
fnp-5883	146	12	complete	complete	ADJ
fnp-5883	146	13	vitrification	vitrification	NOUN
fnp-5883	146	14	is	be	AUX
fnp-5883	146	15	not	not	PART
fnp-5883	146	16	achievable	achievable	ADJ
fnp-5883	146	17	for	for	ADP
fnp-5883	146	18	larger	large	ADJ
fnp-5883	146	19	samples	sample	NOUN
fnp-5883	146	20	.	.	PUNCT
fnp-5883	147	1	therefore	therefore	ADV
fnp-5883	147	2	,	,	PUNCT
fnp-5883	147	3	ice	ice	NOUN
fnp-5883	147	4	crystals	crystal	NOUN
fnp-5883	147	5	will	will	AUX
fnp-5883	147	6	form	form	VERB
fnp-5883	147	7	.	.	PUNCT
fnp-5883	148	1	the	the	DET
fnp-5883	148	2	total	total	ADJ
fnp-5883	148	3	amount	amount	NOUN
fnp-5883	148	4	of	of	ADP
fnp-5883	148	5	ice	ice	NOUN
fnp-5883	148	6	formed	form	VERB
fnp-5883	148	7	is	be	AUX
fnp-5883	148	8	primarily	primarily	ADV
fnp-5883	148	9	dependent	dependent	ADJ
fnp-5883	148	10	on	on	ADP
fnp-5883	148	11	the	the	DET
fnp-5883	148	12	water	water	NOUN
fnp-5883	148	13	content	content	NOUN
fnp-5883	148	14	of	of	ADP
fnp-5883	148	15	the	the	DET
fnp-5883	148	16	tissue	tissue	NOUN
fnp-5883	148	17	and	and	CCONJ
fnp-5883	148	18	the	the	DET
fnp-5883	148	19	final	final	ADJ
fnp-5883	148	20	temperature	temperature	NOUN
fnp-5883	148	21	reached	reach	VERB
fnp-5883	148	22	,	,	PUNCT
fnp-5883	148	23	rather	rather	ADV
fnp-5883	148	24	than	than	ADP
fnp-5883	148	25	on	on	ADP
fnp-5883	148	26	the	the	DET
fnp-5883	148	27	cooling	cool	VERB
fnp-5883	148	28	rate	rate	NOUN
fnp-5883	148	29	.	.	PUNCT
fnp-5883	149	1	for	for	ADP
fnp-5883	149	2	example	example	NOUN
fnp-5883	149	3	,	,	PUNCT
fnp-5883	149	4	in	in	ADP
fnp-5883	149	5	one	one	NUM
fnp-5883	149	6	study	study	NOUN
fnp-5883	149	7	of	of	ADP
fnp-5883	149	8	frozen	frozen	ADJ
fnp-5883	149	9	sponge	sponge	NOUN
fnp-5883	149	10	cake	cake	NOUN
fnp-5883	149	11	,	,	PUNCT
fnp-5883	149	12	the	the	DET
fnp-5883	149	13	total	total	ADJ
fnp-5883	149	14	ice	ice	NOUN
fnp-5883	149	15	volume	volume	NOUN
fnp-5883	149	16	fraction	fraction	NOUN
fnp-5883	149	17	–	–	PUNCT
fnp-5883	149	18	about	about	ADV
fnp-5883	149	19	60	60	NUM
fnp-5883	149	20	 	 	SPACE
fnp-5883	149	21	%	%	NOUN
fnp-5883	149	22	–	–	PUNCT
fnp-5883	149	23	was	be	AUX
fnp-5883	149	24	the	the	DET
fnp-5883	149	25	same	same	ADJ
fnp-5883	149	26	in	in	ADP
fnp-5883	149	27	samples	sample	NOUN
fnp-5883	149	28	frozen	freeze	VERB
fnp-5883	149	29	with	with	ADP
fnp-5883	149	30	fast	fast	ADJ
fnp-5883	149	31	(	(	PUNCT
fnp-5883	149	32	17.2	17.2	NUM
fnp-5883	149	33	 	 	SPACE
fnp-5883	149	34	°	°	ADP
fnp-5883	149	35	c	c	NOUN
fnp-5883	149	36	/	/	SYM
fnp-5883	149	37	min	min	NOUN
fnp-5883	149	38	)	)	PUNCT
fnp-5883	149	39	and	and	CCONJ
fnp-5883	149	40	slow	slow	ADJ
fnp-5883	149	41	(	(	PUNCT
fnp-5883	149	42	0.3	0.3	NUM
fnp-5883	149	43	 	 	SPACE
fnp-5883	149	44	°	°	PROPN
fnp-5883	149	45	c	c	NOUN
fnp-5883	149	46	/	/	SYM
fnp-5883	149	47	min	min	NOUN
fnp-5883	149	48	)	)	PUNCT
fnp-5883	149	49	cooling	cool	VERB
fnp-5883	149	50	rates	rate	NOUN
fnp-5883	149	51	(	(	PUNCT
fnp-5883	149	52	zennoune	zennoune	NOUN
fnp-5883	149	53	et	et	PROPN
fnp-5883	149	54	al	al	PROPN
fnp-5883	149	55	.	.	PROPN
fnp-5883	149	56	,	,	PUNCT
fnp-5883	149	57	2022	2022	NUM
fnp-5883	149	58	)	)	PUNCT
fnp-5883	149	59	.	.	PUNCT
fnp-5883	150	1	however	however	ADV
fnp-5883	150	2	,	,	PUNCT
fnp-5883	150	3	the	the	DET
fnp-5883	150	4	distribution	distribution	NOUN
fnp-5883	150	5	and	and	CCONJ
fnp-5883	150	6	size	size	NOUN
fnp-5883	150	7	of	of	ADP
fnp-5883	150	8	ice	ice	NOUN
fnp-5883	150	9	crystals	crystal	NOUN
fnp-5883	150	10	can	can	AUX
fnp-5883	150	11	differ	differ	VERB
fnp-5883	150	12	substantially	substantially	ADV
fnp-5883	150	13	based	base	VERB
fnp-5883	150	14	on	on	ADP
fnp-5883	150	15	the	the	DET
fnp-5883	150	16	cooling	cool	VERB
fnp-5883	150	17	rate	rate	NOUN
fnp-5883	150	18	,	,	PUNCT
fnp-5883	150	19	which	which	PRON
fnp-5883	150	20	can	can	AUX
fnp-5883	150	21	have	have	VERB
fnp-5883	150	22	important	important	ADJ
fnp-5883	150	23	effects	effect	NOUN
fnp-5883	150	24	on	on	ADP
fnp-5883	150	25	microstructural	microstructural	ADJ
fnp-5883	150	26	preservation	preservation	NOUN
fnp-5883	150	27	.	.	PUNCT
fnp-5883	151	1	relatively	relatively	ADV
fnp-5883	151	2	faster	fast	ADV
fnp-5883	151	3	cooling	cool	VERB
fnp-5883	151	4	results	result	NOUN
fnp-5883	151	5	in	in	ADP
fnp-5883	151	6	the	the	DET
fnp-5883	151	7	formation	formation	NOUN
fnp-5883	151	8	of	of	ADP
fnp-5883	151	9	numerous	numerous	ADJ
fnp-5883	151	10	but	but	CCONJ
fnp-5883	151	11	smaller	small	ADJ
fnp-5883	151	12	ice	ice	NOUN
fnp-5883	151	13	crystals	crystal	NOUN
fnp-5883	151	14	,	,	PUNCT
fnp-5883	151	15	which	which	PRON
fnp-5883	151	16	are	be	AUX
fnp-5883	151	17	less	less	ADV
fnp-5883	151	18	disruptive	disruptive	ADJ
fnp-5883	151	19	to	to	ADP
fnp-5883	151	20	cellular	cellular	ADJ
fnp-5883	151	21	structures	structure	NOUN
fnp-5883	151	22	(	(	PUNCT
fnp-5883	151	23	pérez	pérez	NOUN
fnp-5883	151	24	-	-	PUNCT
fnp-5883	151	25	bermúdez	bermúdez	PROPN
fnp-5883	151	26	et	et	PROPN
fnp-5883	151	27	al	al	PROPN
fnp-5883	151	28	.	.	PROPN
fnp-5883	151	29	,	,	PUNCT
fnp-5883	151	30	2023	2023	NUM
fnp-5883	151	31	)	)	PUNCT
fnp-5883	151	32	.	.	PUNCT
fnp-5883	152	1	this	this	PRON
fnp-5883	152	2	is	be	AUX
fnp-5883	152	3	because	because	SCONJ
fnp-5883	152	4	rapid	rapid	ADJ
fnp-5883	152	5	cooling	cooling	NOUN
fnp-5883	152	6	reduces	reduce	VERB
fnp-5883	152	7	the	the	DET
fnp-5883	152	8	time	time	NOUN
fnp-5883	152	9	available	available	ADJ
fnp-5883	152	10	for	for	ADP
fnp-5883	152	11	ice	ice	NOUN
fnp-5883	152	12	crystal	crystal	NOUN
fnp-5883	152	13	growth	growth	NOUN
fnp-5883	152	14	and	and	CCONJ
fnp-5883	152	15	rearrangement	rearrangement	NOUN
fnp-5883	152	16	.	.	PUNCT
fnp-5883	153	1	smaller	small	ADJ
fnp-5883	153	2	,	,	PUNCT
fnp-5883	153	3	more	more	ADV
fnp-5883	153	4	numerous	numerous	ADJ
fnp-5883	153	5	ice	ice	NOUN
fnp-5883	153	6	crystals	crystal	NOUN
fnp-5883	153	7	that	that	PRON
fnp-5883	153	8	form	form	VERB
fnp-5883	153	9	during	during	ADP
fnp-5883	153	10	rapid	rapid	ADJ
fnp-5883	153	11	cooling	cooling	NOUN
fnp-5883	153	12	are	be	AUX
fnp-5883	153	13	generally	generally	ADV
fnp-5883	153	14	found	find	VERB
fnp-5883	153	15	to	to	PART
fnp-5883	153	16	be	be	AUX
fnp-5883	153	17	less	less	ADV
fnp-5883	153	18	damaging	damaging	ADJ
fnp-5883	153	19	to	to	ADP
fnp-5883	153	20	tissue	tissue	NOUN
fnp-5883	153	21	morphology	morphology	NOUN
fnp-5883	153	22	than	than	ADP
fnp-5883	153	23	larger	large	ADJ
fnp-5883	153	24	crystals	crystal	NOUN
fnp-5883	153	25	that	that	PRON
fnp-5883	153	26	form	form	VERB
fnp-5883	153	27	during	during	ADP
fnp-5883	153	28	slow	slow	ADJ
fnp-5883	153	29	cooling	cooling	NOUN
fnp-5883	153	30	.	.	PUNCT
fnp-5883	154	1	notably	notably	ADV
fnp-5883	154	2	,	,	PUNCT
fnp-5883	154	3	sufficiently	sufficiently	ADV
fnp-5883	154	4	small	small	ADJ
fnp-5883	154	5	ice	ice	NOUN
fnp-5883	154	6	crystals	crystal	NOUN
fnp-5883	154	7	are	be	AUX
fnp-5883	154	8	not	not	PART
fnp-5883	154	9	expected	expect	VERB
fnp-5883	154	10	to	to	PART
fnp-5883	154	11	be	be	AUX
fnp-5883	154	12	visible	visible	ADJ
fnp-5883	154	13	via	via	ADP
fnp-5883	154	14	light	light	ADJ
fnp-5883	154	15	microscopy	microscopy	NOUN
fnp-5883	154	16	(	(	PUNCT
fnp-5883	154	17	szabo	szabo	PROPN
fnp-5883	154	18	and	and	CCONJ
fnp-5883	154	19	burg	burg	PROPN
fnp-5883	154	20	,	,	PUNCT
fnp-5883	154	21	2024	2024	NUM
fnp-5883	154	22	)	)	PUNCT
fnp-5883	154	23	.	.	PUNCT
fnp-5883	155	1	it	it	PRON
fnp-5883	155	2	is	be	AUX
fnp-5883	155	3	also	also	ADV
fnp-5883	155	4	helpful	helpful	ADJ
fnp-5883	155	5	to	to	PART
fnp-5883	155	6	understand	understand	VERB
fnp-5883	155	7	mechanistically	mechanistically	ADV
fnp-5883	155	8	what	what	PRON
fnp-5883	155	9	occurs	occur	VERB
fnp-5883	155	10	during	during	ADP
fnp-5883	155	11	slower	slow	ADJ
fnp-5883	155	12	cooling	cool	VERB
fnp-5883	155	13	processes	process	NOUN
fnp-5883	155	14	without	without	ADP
fnp-5883	155	15	a	a	DET
fnp-5883	155	16	sufficient	sufficient	ADJ
fnp-5883	155	17	concentration	concentration	NOUN
fnp-5883	155	18	of	of	ADP
fnp-5883	155	19	cryoprotectants	cryoprotectant	NOUN
fnp-5883	155	20	to	to	PART
fnp-5883	155	21	vitrify	vitrify	VERB
fnp-5883	155	22	the	the	DET
fnp-5883	155	23	entire	entire	ADJ
fnp-5883	155	24	tissue	tissue	NOUN
fnp-5883	155	25	.	.	PUNCT
fnp-5883	156	1	in	in	ADP
fnp-5883	156	2	this	this	DET
fnp-5883	156	3	situation	situation	NOUN
fnp-5883	156	4	,	,	PUNCT
fnp-5883	156	5	if	if	SCONJ
fnp-5883	156	6	the	the	DET
fnp-5883	156	7	temperature	temperature	NOUN
fnp-5883	156	8	is	be	AUX
fnp-5883	156	9	lowered	lower	VERB
fnp-5883	156	10	to	to	ADP
fnp-5883	156	11	below	below	ADP
fnp-5883	156	12	the	the	DET
fnp-5883	156	13	glass	glass	NOUN
fnp-5883	156	14	transition	transition	NOUN
fnp-5883	156	15	temperature	temperature	NOUN
fnp-5883	156	16	,	,	PUNCT
fnp-5883	156	17	then	then	ADV
fnp-5883	156	18	vitrification	vitrification	NOUN
fnp-5883	156	19	is	be	AUX
fnp-5883	156	20	still	still	ADV
fnp-5883	156	21	expected	expect	VERB
fnp-5883	156	22	to	to	PART
fnp-5883	156	23	occur	occur	VERB
fnp-5883	156	24	in	in	ADP
fnp-5883	156	25	localized	localized	ADJ
fnp-5883	156	26	areas	area	NOUN
fnp-5883	156	27	between	between	ADP
fnp-5883	156	28	pockets	pocket	NOUN
fnp-5883	156	29	of	of	ADP
fnp-5883	156	30	ice	ice	NOUN
fnp-5883	156	31	crystals	crystal	NOUN
fnp-5883	156	32	.	.	PUNCT
fnp-5883	157	1	this	this	PRON
fnp-5883	157	2	is	be	AUX
fnp-5883	157	3	because	because	SCONJ
fnp-5883	157	4	as	as	SCONJ
fnp-5883	157	5	ice	ice	NOUN
fnp-5883	157	6	formation	formation	NOUN
fnp-5883	157	7	progresses	progress	VERB
fnp-5883	157	8	during	during	ADP
fnp-5883	157	9	cooling	cool	VERB
fnp-5883	157	10	,	,	PUNCT
fnp-5883	157	11	the	the	DET
fnp-5883	157	12	concentration	concentration	NOUN
fnp-5883	157	13	of	of	ADP
fnp-5883	157	14	solutes	solute	NOUN
fnp-5883	157	15	in	in	ADP
fnp-5883	157	16	the	the	DET
fnp-5883	157	17	remaining	remain	VERB
fnp-5883	157	18	unfrozen	unfrozen	NOUN
fnp-5883	157	19	areas	area	NOUN
fnp-5883	157	20	eventually	eventually	ADV
fnp-5883	157	21	becomes	become	VERB
fnp-5883	157	22	high	high	ADJ
fnp-5883	157	23	enough	enough	ADV
fnp-5883	157	24	to	to	PART
fnp-5883	157	25	cause	cause	VERB
fnp-5883	157	26	these	these	DET
fnp-5883	157	27	areas	area	NOUN
fnp-5883	157	28	to	to	PART
fnp-5883	157	29	vitrify	vitrify	VERB
fnp-5883	157	30	when	when	SCONJ
fnp-5883	157	31	the	the	DET
fnp-5883	157	32	temperature	temperature	NOUN
fnp-5883	157	33	reaches	reach	VERB
fnp-5883	157	34	the	the	DET
fnp-5883	157	35	glass	glass	NOUN
fnp-5883	157	36	transition	transition	NOUN
fnp-5883	157	37	temperature	temperature	NOUN
fnp-5883	157	38	(	(	PUNCT
fnp-5883	157	39	wowk	wowk	NOUN
fnp-5883	157	40	,	,	PUNCT
fnp-5883	157	41	2010	2010	NUM
fnp-5883	157	42	)	)	PUNCT
fnp-5883	157	43	.	.	PUNCT
fnp-5883	158	1	localized	localize	VERB
fnp-5883	158	2	vitrification	vitrification	NOUN
fnp-5883	158	3	within	within	ADP
fnp-5883	158	4	confined	confine	VERB
fnp-5883	158	5	spaces	space	NOUN
fnp-5883	158	6	during	during	ADP
fnp-5883	158	7	slow	slow	ADJ
fnp-5883	158	8	cooling	cooling	NOUN
fnp-5883	158	9	is	be	AUX
fnp-5883	158	10	particularly	particularly	ADV
fnp-5883	158	11	relevant	relevant	ADJ
fnp-5883	158	12	for	for	ADP
fnp-5883	158	13	intracellular	intracellular	ADJ
fnp-5883	158	14	spaces	space	NOUN
fnp-5883	158	15	,	,	PUNCT
fnp-5883	158	16	which	which	PRON
fnp-5883	158	17	are	be	AUX
fnp-5883	158	18	densely	densely	ADV
fnp-5883	158	19	packed	pack	VERB
fnp-5883	158	20	with	with	ADP
fnp-5883	158	21	proteins	protein	NOUN
fnp-5883	158	22	and	and	CCONJ
fnp-5883	158	23	other	other	ADJ
fnp-5883	158	24	biomolecules	biomolecule	NOUN
fnp-5883	158	25	(	(	PUNCT
fnp-5883	158	26	clarke	clarke	PROPN
fnp-5883	158	27	et	et	PROPN
fnp-5883	158	28	al	al	PROPN
fnp-5883	158	29	.	.	PROPN
fnp-5883	158	30	,	,	PUNCT
fnp-5883	158	31	2013	2013	NUM
fnp-5883	158	32	)	)	PUNCT
fnp-5883	158	33	.	.	PUNCT
fnp-5883	159	1	cryoprotectants	cryoprotectant	NOUN
fnp-5883	159	2	can	can	AUX
fnp-5883	159	3	protect	protect	VERB
fnp-5883	159	4	cells	cell	NOUN
fnp-5883	159	5	from	from	ADP
fnp-5883	159	6	morphological	morphological	ADJ
fnp-5883	159	7	damage	damage	NOUN
fnp-5883	159	8	during	during	ADP
fnp-5883	159	9	this	this	DET
fnp-5883	159	10	process	process	NOUN
fnp-5883	159	11	by	by	ADP
fnp-5883	159	12	limiting	limit	VERB
fnp-5883	159	13	ice	ice	NOUN
fnp-5883	159	14	formation	formation	NOUN
fnp-5883	159	15	and	and	CCONJ
fnp-5883	159	16	preventing	prevent	VERB
fnp-5883	159	17	harmful	harmful	ADJ
fnp-5883	159	18	,	,	PUNCT
fnp-5883	159	19	elevated	elevated	ADJ
fnp-5883	159	20	salt	salt	NOUN
fnp-5883	159	21	concentrations	concentration	NOUN
fnp-5883	159	22	.	.	PUNCT
fnp-5883	160	1	for	for	ADP
fnp-5883	160	2	example	example	NOUN
fnp-5883	160	3	,	,	PUNCT
fnp-5883	160	4	in	in	ADP
fnp-5883	160	5	a	a	DET
fnp-5883	160	6	study	study	NOUN
fnp-5883	160	7	of	of	ADP
fnp-5883	160	8	slowly	slowly	ADV
fnp-5883	160	9	cooling	cool	VERB
fnp-5883	160	10	jurkat	jurkat	PROPN
fnp-5883	160	11	cells	cell	NOUN
fnp-5883	160	12	(	(	PUNCT
fnp-5883	160	13	an	an	DET
fnp-5883	160	14	immortalized	immortalize	VERB
fnp-5883	160	15	t	t	NOUN
fnp-5883	160	16	cell	cell	NOUN
fnp-5883	160	17	line	line	NOUN
fnp-5883	160	18	)	)	PUNCT
fnp-5883	160	19	in	in	ADP
fnp-5883	160	20	the	the	DET
fnp-5883	160	21	presence	presence	NOUN
fnp-5883	160	22	of	of	ADP
fnp-5883	160	23	dmso	dmso	NOUN
fnp-5883	160	24	,	,	PUNCT
fnp-5883	160	25	an	an	DET
fnp-5883	160	26	endothermic	endothermic	ADJ
fnp-5883	160	27	event	event	NOUN
fnp-5883	160	28	was	be	AUX
fnp-5883	160	29	observed	observe	VERB
fnp-5883	160	30	around	around	ADP
fnp-5883	160	31	‑47	‑47	NOUN
fnp-5883	160	32	 	 	SPACE
fnp-5883	160	33	°	°	ADP
fnp-5883	160	34	c	c	NOUN
fnp-5883	160	35	,	,	PUNCT
fnp-5883	160	36	which	which	PRON
fnp-5883	160	37	was	be	AUX
fnp-5883	160	38	attributed	attribute	VERB
fnp-5883	160	39	to	to	ADP
fnp-5883	160	40	a	a	DET
fnp-5883	160	41	colloidal	colloidal	NOUN
fnp-5883	160	42	-	-	PUNCT
fnp-5883	160	43	like	like	ADJ
fnp-5883	160	44	glass	glass	NOUN
fnp-5883	160	45	transition	transition	NOUN
fnp-5883	160	46	in	in	ADP
fnp-5883	160	47	the	the	DET
fnp-5883	160	48	cytoplasm	cytoplasm	NOUN
fnp-5883	160	49	resulting	result	VERB
fnp-5883	160	50	from	from	ADP
fnp-5883	160	51	cellular	cellular	ADJ
fnp-5883	160	52	dehydration	dehydration	NOUN
fnp-5883	160	53	and	and	CCONJ
fnp-5883	160	54	increased	increase	VERB
fnp-5883	160	55	intracellular	intracellular	ADJ
fnp-5883	160	56	macromolecular	macromolecular	NOUN
fnp-5883	160	57	crowding	crowd	VERB
fnp-5883	160	58	(	(	PUNCT
fnp-5883	160	59	meneghel	meneghel	PROPN
fnp-5883	160	60	et	et	PROPN
fnp-5883	160	61	al	al	PROPN
fnp-5883	160	62	.	.	PROPN
fnp-5883	160	63	,	,	PUNCT
fnp-5883	160	64	2019	2019	NUM
fnp-5883	160	65	)	)	PUNCT
fnp-5883	160	66	.	.	PUNCT
fnp-5883	161	1	the	the	DET
fnp-5883	161	2	specific	specific	ADJ
fnp-5883	161	3	temperature	temperature	NOUN
fnp-5883	161	4	of	of	ADP
fnp-5883	161	5	any	any	DET
fnp-5883	161	6	intracellular	intracellular	ADJ
fnp-5883	161	7	glass	glass	NOUN
fnp-5883	161	8	transition	transition	NOUN
fnp-5883	161	9	will	will	AUX
fnp-5883	161	10	vary	vary	VERB
fnp-5883	161	11	depending	depend	VERB
fnp-5883	161	12	on	on	ADP
fnp-5883	161	13	many	many	ADJ
fnp-5883	161	14	factors	factor	NOUN
fnp-5883	161	15	,	,	PUNCT
fnp-5883	161	16	such	such	ADJ
fnp-5883	161	17	as	as	ADP
fnp-5883	161	18	cell	cell	NOUN
fnp-5883	161	19	type	type	NOUN
fnp-5883	161	20	,	,	PUNCT
fnp-5883	161	21	protein	protein	NOUN
fnp-5883	161	22	content	content	NOUN
fnp-5883	161	23	,	,	PUNCT
fnp-5883	161	24	solute	solute	ADJ
fnp-5883	161	25	concentration	concentration	NOUN
fnp-5883	161	26	,	,	PUNCT
fnp-5883	161	27	and	and	CCONJ
fnp-5883	161	28	the	the	DET
fnp-5883	161	29	concentration	concentration	NOUN
fnp-5883	161	30	of	of	ADP
fnp-5883	161	31	any	any	DET
fnp-5883	161	32	cryoprotectants	cryoprotectant	NOUN
fnp-5883	161	33	.	.	PUNCT
fnp-5883	162	1	in	in	ADP
fnp-5883	162	2	the	the	DET
fnp-5883	162	3	context	context	NOUN
fnp-5883	162	4	of	of	ADP
fnp-5883	162	5	brain	brain	NOUN
fnp-5883	162	6	tissue	tissue	NOUN
fnp-5883	162	7	,	,	PUNCT
fnp-5883	162	8	even	even	ADV
fnp-5883	162	9	if	if	SCONJ
fnp-5883	162	10	some	some	DET
fnp-5883	162	11	degree	degree	NOUN
fnp-5883	162	12	of	of	ADP
fnp-5883	162	13	intracellular	intracellular	ADJ
fnp-5883	162	14	vitrification	vitrification	NOUN
fnp-5883	162	15	occurs	occur	VERB
fnp-5883	162	16	,	,	PUNCT
fnp-5883	162	17	the	the	DET
fnp-5883	162	18	fine	fine	ADJ
fnp-5883	162	19	cell	cell	NOUN
fnp-5883	162	20	processes	process	NOUN
fnp-5883	162	21	or	or	CCONJ
fnp-5883	162	22	cell	cell	NOUN
fnp-5883	162	23	-	-	PUNCT
fnp-5883	162	24	cell	cell	NOUN
fnp-5883	162	25	connections	connection	NOUN
fnp-5883	162	26	may	may	AUX
fnp-5883	162	27	be	be	AUX
fnp-5883	162	28	mechanically	mechanically	ADV
fnp-5883	162	29	damaged	damage	VERB
fnp-5883	162	30	if	if	SCONJ
fnp-5883	162	31	extracellular	extracellular	ADJ
fnp-5883	162	32	ice	ice	NOUN
fnp-5883	162	33	is	be	AUX
fnp-5883	162	34	formed	form	VERB
fnp-5883	162	35	.	.	PUNCT
fnp-5883	163	1	eutectic	eutectic	ADJ
fnp-5883	163	2	crystallization	crystallization	NOUN
fnp-5883	163	3	although	although	SCONJ
fnp-5883	163	4	not	not	PART
fnp-5883	163	5	commonly	commonly	ADV
fnp-5883	163	6	discussed	discuss	VERB
fnp-5883	163	7	,	,	PUNCT
fnp-5883	163	8	we	we	PRON
fnp-5883	163	9	note	note	VERB
fnp-5883	163	10	for	for	ADP
fnp-5883	163	11	completeness	completeness	NOUN
fnp-5883	163	12	that	that	PRON
fnp-5883	163	13	eutectic	eutectic	ADJ
fnp-5883	163	14	crystallization	crystallization	NOUN
fnp-5883	163	15	can	can	AUX
fnp-5883	163	16	occur	occur	VERB
fnp-5883	163	17	during	during	ADP
fnp-5883	163	18	cryopreservation	cryopreservation	NOUN
fnp-5883	163	19	in	in	ADP
fnp-5883	163	20	addition	addition	NOUN
fnp-5883	163	21	to	to	ADP
fnp-5883	163	22	the	the	DET
fnp-5883	163	23	crystallization	crystallization	NOUN
fnp-5883	163	24	of	of	ADP
fnp-5883	163	25	water	water	NOUN
fnp-5883	163	26	forming	form	VERB
fnp-5883	163	27	ice	ice	NOUN
fnp-5883	163	28	.	.	PUNCT
fnp-5883	164	1	a	a	DET
fnp-5883	164	2	eutectic	eutectic	ADJ
fnp-5883	164	3	system	system	NOUN
fnp-5883	164	4	is	be	AUX
fnp-5883	164	5	a	a	DET
fnp-5883	164	6	mixture	mixture	NOUN
fnp-5883	164	7	of	of	ADP
fnp-5883	164	8	substances	substance	NOUN
fnp-5883	164	9	that	that	PRON
fnp-5883	164	10	has	have	VERB
fnp-5883	164	11	a	a	DET
fnp-5883	164	12	melting	melting	NOUN
fnp-5883	164	13	point	point	NOUN
fnp-5883	164	14	below	below	ADP
fnp-5883	164	15	either	either	PRON
fnp-5883	164	16	of	of	ADP
fnp-5883	164	17	the	the	DET
fnp-5883	164	18	original	original	ADJ
fnp-5883	164	19	substances	substance	NOUN
fnp-5883	164	20	.	.	PUNCT
fnp-5883	165	1	when	when	SCONJ
fnp-5883	165	2	the	the	DET
fnp-5883	165	3	melting	melting	NOUN
fnp-5883	165	4	point	point	NOUN
fnp-5883	165	5	of	of	ADP
fnp-5883	165	6	this	this	DET
fnp-5883	165	7	mixture	mixture	NOUN
fnp-5883	165	8	is	be	AUX
fnp-5883	165	9	reached	reach	VERB
fnp-5883	165	10	,	,	PUNCT
fnp-5883	165	11	the	the	DET
fnp-5883	165	12	eutectic	eutectic	ADJ
fnp-5883	165	13	system	system	NOUN
fnp-5883	165	14	can	can	AUX
fnp-5883	165	15	solidify	solidify	VERB
fnp-5883	165	16	.	.	PUNCT
fnp-5883	166	1	in	in	ADP
fnp-5883	166	2	practical	practical	ADJ
fnp-5883	166	3	terms	term	NOUN
fnp-5883	166	4	,	,	PUNCT
fnp-5883	166	5	sodium	sodium	NOUN
fnp-5883	166	6	chloride	chloride	NOUN
fnp-5883	166	7	tends	tend	VERB
fnp-5883	166	8	to	to	PART
fnp-5883	166	9	be	be	AUX
fnp-5883	166	10	the	the	DET
fnp-5883	166	11	main	main	ADJ
fnp-5883	166	12	relevant	relevant	ADJ
fnp-5883	166	13	electrolyte	electrolyte	NOUN
fnp-5883	166	14	in	in	ADP
fnp-5883	166	15	biological	biological	ADJ
fnp-5883	166	16	tissues	tissue	NOUN
fnp-5883	166	17	like	like	ADP
fnp-5883	166	18	the	the	DET
fnp-5883	166	19	brain	brain	NOUN
fnp-5883	166	20	(	(	PUNCT
fnp-5883	166	21	han	han	NOUN
fnp-5883	166	22	and	and	CCONJ
fnp-5883	166	23	bischof	bischof	NOUN
fnp-5883	166	24	,	,	PUNCT
fnp-5883	166	25	2004	2004	NUM
fnp-5883	166	26	)	)	PUNCT
fnp-5883	166	27	.	.	PUNCT
fnp-5883	167	1	as	as	ADP
fnp-5883	167	2	ice	ice	NOUN
fnp-5883	167	3	forms	form	NOUN
fnp-5883	167	4	during	during	ADP
fnp-5883	167	5	cryopreservation	cryopreservation	NOUN
fnp-5883	167	6	,	,	PUNCT
fnp-5883	167	7	the	the	DET
fnp-5883	167	8	water	water	NOUN
fnp-5883	167	9	-	-	PUNCT
fnp-5883	167	10	sodium	sodium	NOUN
fnp-5883	167	11	chloride	chloride	NOUN
fnp-5883	167	12	solution	solution	NOUN
fnp-5883	167	13	increases	increase	VERB
fnp-5883	167	14	in	in	ADP
fnp-5883	167	15	concentration	concentration	NOUN
fnp-5883	167	16	and	and	CCONJ
fnp-5883	167	17	it	it	PRON
fnp-5883	167	18	can	can	AUX
fnp-5883	167	19	form	form	VERB
fnp-5883	167	20	a	a	DET
fnp-5883	167	21	eutectic	eutectic	ADJ
fnp-5883	167	22	system	system	NOUN
fnp-5883	167	23	because	because	SCONJ
fnp-5883	167	24	the	the	DET
fnp-5883	167	25	mixture	mixture	NOUN
fnp-5883	167	26	has	have	VERB
fnp-5883	167	27	a	a	DET
fnp-5883	167	28	melting	melting	NOUN
fnp-5883	167	29	point	point	NOUN
fnp-5883	167	30	lower	low	ADJ
fnp-5883	167	31	than	than	ADP
fnp-5883	167	32	either	either	PRON
fnp-5883	167	33	of	of	ADP
fnp-5883	167	34	the	the	DET
fnp-5883	167	35	individual	individual	ADJ
fnp-5883	167	36	components	component	NOUN
fnp-5883	167	37	.	.	PUNCT
fnp-5883	168	1	in	in	ADP
fnp-5883	168	2	one	one	NUM
fnp-5883	168	3	study	study	NOUN
fnp-5883	168	4	,	,	PUNCT
fnp-5883	168	5	eutectic	eutectic	ADJ
fnp-5883	168	6	crystallization	crystallization	NOUN
fnp-5883	168	7	of	of	ADP
fnp-5883	168	8	water	water	NOUN
fnp-5883	168	9	-	-	PUNCT
fnp-5883	168	10	sodium	sodium	NOUN
fnp-5883	168	11	chloride	chloride	NOUN
fnp-5883	168	12	solutions	solution	NOUN
fnp-5883	168	13	–	–	PUNCT
fnp-5883	168	14	forming	form	VERB
fnp-5883	168	15	hydrohalite	hydrohalite	NOUN
fnp-5883	168	16	–	–	PUNCT
fnp-5883	168	17	occurred	occur	VERB
fnp-5883	168	18	at	at	ADP
fnp-5883	168	19	approximately	approximately	ADV
fnp-5883	168	20	‑30	‑30	NUM
fnp-5883	168	21	 	 	SPACE
fnp-5883	168	22	°	°	NOUN
fnp-5883	168	23	c	c	NOUN
fnp-5883	168	24	,	,	PUNCT
fnp-5883	168	25	but	but	CCONJ
fnp-5883	168	26	this	this	PRON
fnp-5883	168	27	may	may	AUX
fnp-5883	168	28	differ	differ	VERB
fnp-5883	168	29	based	base	VERB
fnp-5883	168	30	on	on	ADP
fnp-5883	168	31	the	the	DET
fnp-5883	168	32	cooling	cool	VERB
fnp-5883	168	33	rate	rate	NOUN
fnp-5883	168	34	(	(	PUNCT
fnp-5883	168	35	han	han	NOUN
fnp-5883	168	36	and	and	CCONJ
fnp-5883	168	37	bischof	bischof	NOUN
fnp-5883	168	38	,	,	PUNCT
fnp-5883	168	39	2004	2004	NUM
fnp-5883	168	40	)	)	PUNCT
fnp-5883	168	41	.	.	PUNCT
fnp-5883	169	1	in	in	ADP
fnp-5883	169	2	yeast	yeast	NOUN
fnp-5883	169	3	cells	cell	NOUN
fnp-5883	169	4	,	,	PUNCT
fnp-5883	169	5	hydrohalite	hydrohalite	NOUN
fnp-5883	169	6	has	have	AUX
fnp-5883	169	7	been	be	AUX
fnp-5883	169	8	found	find	VERB
fnp-5883	169	9	primarily	primarily	ADV
fnp-5883	169	10	around	around	ADP
fnp-5883	169	11	cell	cell	NOUN
fnp-5883	169	12	membranes	membrane	NOUN
fnp-5883	169	13	(	(	PUNCT
fnp-5883	169	14	okotrub	okotrub	VERB
fnp-5883	169	15	and	and	CCONJ
fnp-5883	169	16	surovtsev	surovtsev	NOUN
fnp-5883	169	17	,	,	PUNCT
fnp-5883	169	18	2013	2013	NUM
fnp-5883	169	19	)	)	PUNCT
fnp-5883	169	20	.	.	PUNCT
fnp-5883	170	1	such	such	ADJ
fnp-5883	170	2	eutectic	eutectic	ADJ
fnp-5883	170	3	crystallization	crystallization	NOUN
fnp-5883	170	4	can	can	AUX
fnp-5883	170	5	cause	cause	VERB
fnp-5883	170	6	damage	damage	NOUN
fnp-5883	170	7	to	to	ADP
fnp-5883	170	8	cellular	cellular	ADJ
fnp-5883	170	9	structures	structure	NOUN
fnp-5883	170	10	beyond	beyond	ADP
fnp-5883	170	11	that	that	PRON
fnp-5883	170	12	caused	cause	VERB
fnp-5883	170	13	by	by	ADP
fnp-5883	170	14	ice	ice	NOUN
fnp-5883	170	15	formation	formation	NOUN
fnp-5883	170	16	alone	alone	ADV
fnp-5883	170	17	(	(	PUNCT
fnp-5883	170	18	han	han	NOUN
fnp-5883	170	19	and	and	CCONJ
fnp-5883	170	20	bischof	bischof	NOUN
fnp-5883	170	21	,	,	PUNCT
fnp-5883	170	22	2004	2004	NUM
fnp-5883	170	23	;	;	PUNCT
fnp-5883	170	24	klbik	klbik	PROPN
fnp-5883	170	25	et	et	PROPN
fnp-5883	170	26	al	al	PROPN
fnp-5883	170	27	.	.	PROPN
fnp-5883	170	28	,	,	PUNCT
fnp-5883	170	29	2022	2022	NUM
fnp-5883	170	30	)	)	PUNCT
fnp-5883	170	31	.	.	PUNCT
fnp-5883	171	1	it	it	PRON
fnp-5883	171	2	is	be	AUX
fnp-5883	171	3	unclear	unclear	ADJ
fnp-5883	171	4	how	how	SCONJ
fnp-5883	171	5	much	much	ADJ
fnp-5883	171	6	eutectic	eutectic	ADJ
fnp-5883	171	7	crystallization	crystallization	NOUN
fnp-5883	171	8	occurs	occur	VERB
fnp-5883	171	9	in	in	ADP
fnp-5883	171	10	brain	brain	NOUN
fnp-5883	171	11	tissue	tissue	NOUN
fnp-5883	171	12	during	during	ADP
fnp-5883	171	13	cryopreservation	cryopreservation	NOUN
fnp-5883	171	14	.	.	PUNCT
fnp-5883	172	1	however	however	ADV
fnp-5883	172	2	,	,	PUNCT
fnp-5883	172	3	it	it	PRON
fnp-5883	172	4	theoretically	theoretically	ADV
fnp-5883	172	5	could	could	AUX
fnp-5883	172	6	pose	pose	VERB
fnp-5883	172	7	additional	additional	ADJ
fnp-5883	172	8	challenges	challenge	NOUN
fnp-5883	172	9	for	for	ADP
fnp-5883	172	10	maintaining	maintain	VERB
fnp-5883	172	11	cellular	cellular	ADJ
fnp-5883	172	12	morphology	morphology	NOUN
fnp-5883	172	13	.	.	PUNCT
fnp-5883	173	1	notably	notably	ADV
fnp-5883	173	2	,	,	PUNCT
fnp-5883	173	3	the	the	DET
fnp-5883	173	4	use	use	NOUN
fnp-5883	173	5	of	of	ADP
fnp-5883	173	6	cryoprotectants	cryoprotectant	NOUN
fnp-5883	173	7	or	or	CCONJ
fnp-5883	173	8	the	the	DET
fnp-5883	173	9	application	application	NOUN
fnp-5883	173	10	of	of	ADP
fnp-5883	173	11	very	very	ADV
fnp-5883	173	12	rapid	rapid	ADJ
fnp-5883	173	13	freezing	freezing	NOUN
fnp-5883	173	14	techniques	technique	NOUN
fnp-5883	173	15	can	can	AUX
fnp-5883	173	16	potentially	potentially	ADV
fnp-5883	173	17	prevent	prevent	VERB
fnp-5883	173	18	eutectic	eutectic	ADJ
fnp-5883	173	19	crystallization	crystallization	NOUN
fnp-5883	173	20	.	.	PUNCT
fnp-5883	174	1	cellular	cellular	ADJ
fnp-5883	174	2	decomposition	decomposition	NOUN
fnp-5883	174	3	another	another	DET
fnp-5883	174	4	issue	issue	NOUN
fnp-5883	174	5	during	during	ADP
fnp-5883	174	6	cryopreservation	cryopreservation	NOUN
fnp-5883	174	7	is	be	AUX
fnp-5883	174	8	cellular	cellular	ADJ
fnp-5883	174	9	decomposition	decomposition	NOUN
fnp-5883	174	10	by	by	ADP
fnp-5883	174	11	autolysis	autolysis	NOUN
fnp-5883	174	12	(	(	PUNCT
fnp-5883	174	13	self	self	NOUN
fnp-5883	174	14	-	-	PUNCT
fnp-5883	174	15	digestion	digestion	NOUN
fnp-5883	174	16	by	by	ADP
fnp-5883	174	17	cellular	cellular	ADJ
fnp-5883	174	18	enzymes	enzyme	NOUN
fnp-5883	174	19	)	)	PUNCT
fnp-5883	174	20	and	and	CCONJ
fnp-5883	174	21	putrefaction	putrefaction	NOUN
fnp-5883	174	22	(	(	PUNCT
fnp-5883	174	23	bacterial	bacterial	ADJ
fnp-5883	174	24	breakdown	breakdown	NOUN
fnp-5883	174	25	of	of	ADP
fnp-5883	174	26	tissues	tissue	NOUN
fnp-5883	174	27	)	)	PUNCT
fnp-5883	174	28	.	.	PUNCT
fnp-5883	175	1	these	these	DET
fnp-5883	175	2	processes	process	NOUN
fnp-5883	175	3	can	can	AUX
fnp-5883	175	4	occur	occur	VERB
fnp-5883	175	5	during	during	ADP
fnp-5883	175	6	cooling	cool	VERB
fnp-5883	175	7	and	and	CCONJ
fnp-5883	175	8	thawing	thawing	NOUN
fnp-5883	175	9	of	of	ADP
fnp-5883	175	10	large	large	ADJ
fnp-5883	175	11	brain	brain	NOUN
fnp-5883	175	12	tissue	tissue	NOUN
fnp-5883	175	13	samples	sample	NOUN
fnp-5883	175	14	because	because	SCONJ
fnp-5883	175	15	they	they	PRON
fnp-5883	175	16	do	do	AUX
fnp-5883	175	17	not	not	PART
fnp-5883	175	18	cool	cool	VERB
fnp-5883	175	19	instantaneously	instantaneously	ADV
fnp-5883	175	20	,	,	PUNCT
fnp-5883	175	21	due	due	ADP
fnp-5883	175	22	to	to	ADP
fnp-5883	175	23	the	the	DET
fnp-5883	175	24	limits	limit	NOUN
fnp-5883	175	25	of	of	ADP
fnp-5883	175	26	convective	convective	ADJ
fnp-5883	175	27	heat	heat	NOUN
fnp-5883	175	28	transfer	transfer	NOUN
fnp-5883	175	29	(	(	PUNCT
fnp-5883	175	30	pontén	pontén	NOUN
fnp-5883	175	31	et	et	PROPN
fnp-5883	175	32	al	al	PROPN
fnp-5883	175	33	.	.	PROPN
fnp-5883	175	34	,	,	PUNCT
fnp-5883	175	35	1973	1973	NUM
fnp-5883	175	36	)	)	PUNCT
fnp-5883	175	37	.	.	PUNCT
fnp-5883	176	1	autolysis	autolysis	NOUN
fnp-5883	176	2	begins	begin	VERB
fnp-5883	176	3	immediately	immediately	ADV
fnp-5883	176	4	after	after	ADP
fnp-5883	176	5	death	death	NOUN
fnp-5883	176	6	when	when	SCONJ
fnp-5883	176	7	cellular	cellular	ADJ
fnp-5883	176	8	enzymes	enzyme	NOUN
fnp-5883	176	9	are	be	AUX
fnp-5883	176	10	released	release	VERB
fnp-5883	176	11	,	,	PUNCT
fnp-5883	176	12	while	while	SCONJ
fnp-5883	176	13	putrefaction	putrefaction	NOUN
fnp-5883	176	14	typically	typically	ADV
fnp-5883	176	15	starts	start	VERB
fnp-5883	176	16	days	day	NOUN
fnp-5883	176	17	later	later	ADV
fnp-5883	176	18	when	when	SCONJ
fnp-5883	176	19	bacteria	bacteria	NOUN
fnp-5883	176	20	proliferate	proliferate	VERB
fnp-5883	176	21	.	.	PUNCT
fnp-5883	177	1	importantly	importantly	ADV
fnp-5883	177	2	,	,	PUNCT
fnp-5883	177	3	cellular	cellular	ADJ
fnp-5883	177	4	decomposition	decomposition	NOUN
fnp-5883	177	5	is	be	AUX
fnp-5883	177	6	dramatically	dramatically	ADV
fnp-5883	177	7	inhibited	inhibit	VERB
fnp-5883	177	8	by	by	ADP
fnp-5883	177	9	lower	low	ADJ
fnp-5883	177	10	temperatures	temperature	NOUN
fnp-5883	177	11	(	(	PUNCT
fnp-5883	177	12	krassner	krassner	NOUN
fnp-5883	177	13	et	et	PROPN
fnp-5883	177	14	al	al	PROPN
fnp-5883	177	15	.	.	PROPN
fnp-5883	177	16	,	,	PUNCT
fnp-5883	177	17	2023	2023	NUM
fnp-5883	177	18	)	)	PUNCT
fnp-5883	177	19	.	.	PUNCT
fnp-5883	178	1	this	this	PRON
fnp-5883	178	2	is	be	AUX
fnp-5883	178	3	another	another	DET
fnp-5883	178	4	reason	reason	NOUN
fnp-5883	178	5	that	that	PRON
fnp-5883	178	6	swift	swift	ADJ
fnp-5883	178	7	cooling	cooling	NOUN
fnp-5883	178	8	is	be	AUX
fnp-5883	178	9	critical	critical	ADJ
fnp-5883	178	10	in	in	ADP
fnp-5883	178	11	cryopreservation	cryopreservation	NOUN
fnp-5883	178	12	protocols	protocol	NOUN
fnp-5883	178	13	.	.	PUNCT
fnp-5883	179	1	fractures	fracture	VERB
fnp-5883	179	2	one	one	NUM
fnp-5883	179	3	downside	downside	NOUN
fnp-5883	179	4	of	of	ADP
fnp-5883	179	5	cooling	cool	VERB
fnp-5883	179	6	to	to	ADP
fnp-5883	179	7	very	very	ADV
fnp-5883	179	8	low	low	ADJ
fnp-5883	179	9	temperatures	temperature	NOUN
fnp-5883	179	10	is	be	AUX
fnp-5883	179	11	the	the	DET
fnp-5883	179	12	possibility	possibility	NOUN
fnp-5883	179	13	of	of	ADP
fnp-5883	179	14	fractures	fracture	NOUN
fnp-5883	179	15	.	.	PUNCT
fnp-5883	180	1	fracturing	fracturing	NOUN
fnp-5883	180	2	can	can	AUX
fnp-5883	180	3	occur	occur	VERB
fnp-5883	180	4	in	in	ADP
fnp-5883	180	5	tissues	tissue	NOUN
fnp-5883	180	6	or	or	CCONJ
fnp-5883	180	7	organs	organ	NOUN
fnp-5883	180	8	that	that	PRON
fnp-5883	180	9	are	be	AUX
fnp-5883	180	10	vitrified	vitrify	VERB
fnp-5883	180	11	or	or	CCONJ
fnp-5883	180	12	frozen	frozen	ADJ
fnp-5883	180	13	.	.	PUNCT
fnp-5883	181	1	it	it	PRON
fnp-5883	181	2	has	have	AUX
fnp-5883	181	3	been	be	AUX
fnp-5883	181	4	attributed	attribute	VERB
fnp-5883	181	5	to	to	ADP
fnp-5883	181	6	thermal	thermal	ADJ
fnp-5883	181	7	gradients	gradient	NOUN
fnp-5883	181	8	–	–	PUNCT
fnp-5883	181	9	i.e.	i.e.	X
fnp-5883	181	10	differences	difference	NOUN
fnp-5883	181	11	in	in	ADP
fnp-5883	181	12	temperature	temperature	NOUN
fnp-5883	181	13	in	in	ADP
fnp-5883	181	14	different	different	ADJ
fnp-5883	181	15	parts	part	NOUN
fnp-5883	181	16	of	of	ADP
fnp-5883	181	17	the	the	DET
fnp-5883	181	18	organ	organ	NOUN
fnp-5883	181	19	(	(	PUNCT
fnp-5883	181	20	woods	wood	NOUN
fnp-5883	181	21	and	and	CCONJ
fnp-5883	181	22	mullen	mullen	NOUN
fnp-5883	181	23	,	,	PUNCT
fnp-5883	181	24	2016	2016	NUM
fnp-5883	181	25	)	)	PUNCT
fnp-5883	181	26	.	.	PUNCT
fnp-5883	182	1	it	it	PRON
fnp-5883	182	2	seems	seem	VERB
fnp-5883	182	3	to	to	PART
fnp-5883	182	4	be	be	AUX
fnp-5883	182	5	more	more	ADV
fnp-5883	182	6	common	common	ADJ
fnp-5883	182	7	in	in	ADP
fnp-5883	182	8	larger	large	ADJ
fnp-5883	182	9	specimens	specimen	NOUN
fnp-5883	182	10	.	.	PUNCT
fnp-5883	183	1	one	one	NUM
fnp-5883	183	2	study	study	NOUN
fnp-5883	183	3	found	find	VERB
fnp-5883	183	4	that	that	SCONJ
fnp-5883	183	5	brain	brain	NOUN
fnp-5883	183	6	tissue	tissue	NOUN
fnp-5883	183	7	blocks	block	NOUN
fnp-5883	183	8	of	of	ADP
fnp-5883	183	9	larger	large	ADJ
fnp-5883	183	10	than	than	ADP
fnp-5883	183	11	2.5	2.5	NUM
fnp-5883	183	12	to	to	PART
fnp-5883	183	13	3.0	3.0	NUM
fnp-5883	183	14	 	 	SPACE
fnp-5883	183	15	cm	cm	NOUN
fnp-5883	183	16	tend	tend	VERB
fnp-5883	183	17	to	to	PART
fnp-5883	183	18	fragment	fragment	VERB
fnp-5883	183	19	when	when	SCONJ
fnp-5883	183	20	immersed	immerse	VERB
fnp-5883	183	21	in	in	ADP
fnp-5883	183	22	liquid	liquid	ADJ
fnp-5883	183	23	nitrogen	nitrogen	NOUN
fnp-5883	183	24	-	-	PUNCT
fnp-5883	183	25	cooled	cool	VERB
fnp-5883	183	26	liquid	liquid	ADJ
fnp-5883	183	27	isopentane	isopentane	NOUN
fnp-5883	183	28	(	(	PUNCT
fnp-5883	183	29	vonsattel	vonsattel	NOUN
fnp-5883	183	30	et	et	PROPN
fnp-5883	183	31	al	al	PROPN
fnp-5883	183	32	.	.	PROPN
fnp-5883	183	33	,	,	PUNCT
fnp-5883	183	34	2008	2008	NUM
fnp-5883	183	35	)	)	PUNCT
fnp-5883	183	36	.	.	PUNCT
fnp-5883	184	1	fracturing	fracturing	NOUN
fnp-5883	184	2	has	have	AUX
fnp-5883	184	3	also	also	ADV
fnp-5883	184	4	been	be	AUX
fnp-5883	184	5	reported	report	VERB
fnp-5883	184	6	when	when	SCONJ
fnp-5883	184	7	whole	whole	ADJ
fnp-5883	184	8	rodent	rodent	ADJ
fnp-5883	184	9	brains	brain	NOUN
fnp-5883	184	10	are	be	AUX
fnp-5883	184	11	cooled	cool	VERB
fnp-5883	184	12	in	in	ADP
fnp-5883	184	13	liquid	liquid	ADJ
fnp-5883	184	14	nitrogen	nitrogen	NOUN
fnp-5883	184	15	(	(	PUNCT
fnp-5883	184	16	peters	peters	PROPN
fnp-5883	184	17	,	,	PUNCT
fnp-5883	184	18	2010	2010	NUM
fnp-5883	184	19	)	)	PUNCT
fnp-5883	184	20	.	.	PUNCT
fnp-5883	185	1	fractures	fracture	NOUN
fnp-5883	185	2	are	be	AUX
fnp-5883	185	3	more	more	ADV
fnp-5883	185	4	likely	likely	ADJ
fnp-5883	185	5	to	to	PART
fnp-5883	185	6	occur	occur	VERB
fnp-5883	185	7	at	at	ADP
fnp-5883	185	8	lower	low	ADJ
fnp-5883	185	9	temperatures	temperature	NOUN
fnp-5883	185	10	,	,	PUNCT
fnp-5883	185	11	as	as	SCONJ
fnp-5883	185	12	thermal	thermal	ADJ
fnp-5883	185	13	stress	stress	NOUN
fnp-5883	185	14	accumulates	accumulate	VERB
fnp-5883	185	15	.	.	PUNCT
fnp-5883	186	1	one	one	NUM
fnp-5883	186	2	study	study	NOUN
fnp-5883	186	3	found	find	VERB
fnp-5883	186	4	that	that	SCONJ
fnp-5883	186	5	thermal	thermal	ADJ
fnp-5883	186	6	stress	stress	NOUN
fnp-5883	186	7	in	in	ADP
fnp-5883	186	8	a	a	DET
fnp-5883	186	9	cryoprotective	cryoprotective	ADJ
fnp-5883	186	10	solution	solution	NOUN
fnp-5883	186	11	is	be	AUX
fnp-5883	186	12	negligible	negligible	ADJ
fnp-5883	186	13	until	until	ADP
fnp-5883	186	14	a	a	DET
fnp-5883	186	15	temperature	temperature	NOUN
fnp-5883	186	16	of	of	ADP
fnp-5883	186	17	around	around	ADV
fnp-5883	186	18	5–10	5–10	PROPN
fnp-5883	186	19	 	 	SPACE
fnp-5883	186	20	°	°	ADP
fnp-5883	186	21	c	c	NOUN
fnp-5883	186	22	above	above	ADP
fnp-5883	186	23	the	the	DET
fnp-5883	186	24	glass	glass	NOUN
fnp-5883	186	25	transition	transition	NOUN
fnp-5883	186	26	temperature	temperature	NOUN
fnp-5883	186	27	,	,	PUNCT
fnp-5883	186	28	depending	depend	VERB
fnp-5883	186	29	on	on	ADP
fnp-5883	186	30	the	the	DET
fnp-5883	186	31	viscosity	viscosity	NOUN
fnp-5883	186	32	of	of	ADP
fnp-5883	186	33	the	the	DET
fnp-5883	186	34	solution	solution	NOUN
fnp-5883	186	35	(	(	PUNCT
fnp-5883	186	36	rabin	rabin	PROPN
fnp-5883	186	37	et	et	PROPN
fnp-5883	186	38	al	al	PROPN
fnp-5883	186	39	.	.	PROPN
fnp-5883	186	40	,	,	PUNCT
fnp-5883	186	41	2006	2006	NUM
fnp-5883	186	42	)	)	PUNCT
fnp-5883	186	43	.	.	PUNCT
fnp-5883	187	1	slower	slow	ADJ
fnp-5883	187	2	cooling	cool	VERB
fnp-5883	187	3	rates	rate	NOUN
fnp-5883	187	4	allow	allow	VERB
fnp-5883	187	5	more	more	ADJ
fnp-5883	187	6	time	time	NOUN
fnp-5883	187	7	for	for	SCONJ
fnp-5883	187	8	temperature	temperature	NOUN
fnp-5883	187	9	to	to	PART
fnp-5883	187	10	equilibrate	equilibrate	VERB
fnp-5883	187	11	across	across	ADP
fnp-5883	187	12	different	different	ADJ
fnp-5883	187	13	areas	area	NOUN
fnp-5883	187	14	of	of	ADP
fnp-5883	187	15	the	the	DET
fnp-5883	187	16	organ	organ	NOUN
fnp-5883	187	17	or	or	CCONJ
fnp-5883	187	18	tissue	tissue	NOUN
fnp-5883	187	19	,	,	PUNCT
fnp-5883	187	20	lowering	lower	VERB
fnp-5883	187	21	the	the	DET
fnp-5883	187	22	magnitude	magnitude	NOUN
fnp-5883	187	23	of	of	ADP
fnp-5883	187	24	thermal	thermal	ADJ
fnp-5883	187	25	gradients	gradient	NOUN
fnp-5883	187	26	.	.	PUNCT
fnp-5883	188	1	while	while	SCONJ
fnp-5883	188	2	slower	slower	ADJ
fnp-5883	188	3	cooling	cool	VERB
fnp-5883	188	4	rates	rate	NOUN
fnp-5883	188	5	are	be	AUX
fnp-5883	188	6	associated	associate	VERB
fnp-5883	188	7	with	with	ADP
fnp-5883	188	8	lower	low	ADJ
fnp-5883	188	9	fracture	fracture	NOUN
fnp-5883	188	10	rates	rate	NOUN
fnp-5883	188	11	,	,	PUNCT
fnp-5883	188	12	this	this	DET
fnp-5883	188	13	effect	effect	NOUN
fnp-5883	188	14	appears	appear	VERB
fnp-5883	188	15	to	to	PART
fnp-5883	188	16	be	be	AUX
fnp-5883	188	17	relatively	relatively	ADV
fnp-5883	188	18	modest	modest	ADJ
fnp-5883	188	19	.	.	PUNCT
fnp-5883	189	1	for	for	ADP
fnp-5883	189	2	example	example	NOUN
fnp-5883	189	3	,	,	PUNCT
fnp-5883	189	4	in	in	ADP
fnp-5883	189	5	one	one	NUM
fnp-5883	189	6	study	study	NOUN
fnp-5883	189	7	,	,	PUNCT
fnp-5883	189	8	increasing	increase	VERB
fnp-5883	189	9	the	the	DET
fnp-5883	189	10	cooling	cool	VERB
fnp-5883	189	11	rate	rate	NOUN
fnp-5883	189	12	by	by	ADP
fnp-5883	189	13	three	three	NUM
fnp-5883	189	14	orders	order	NOUN
fnp-5883	189	15	of	of	ADP
fnp-5883	189	16	magnitude	magnitude	NOUN
fnp-5883	189	17	,	,	PUNCT
fnp-5883	189	18	from	from	ADP
fnp-5883	189	19	0.5	0.5	NUM
fnp-5883	189	20	 	 	SPACE
fnp-5883	189	21	°	°	PROPN
fnp-5883	189	22	c	c	NOUN
fnp-5883	189	23	/	/	SYM
fnp-5883	189	24	min	min	NOUN
fnp-5883	189	25	to	to	ADP
fnp-5883	189	26	500	500	NUM
fnp-5883	189	27	 	 	SPACE
fnp-5883	189	28	°	°	PROPN
fnp-5883	189	29	c	c	PROPN
fnp-5883	189	30	/	/	SYM
fnp-5883	189	31	min	min	NOUN
fnp-5883	189	32	,	,	PUNCT
fnp-5883	189	33	decreased	decrease	VERB
fnp-5883	189	34	the	the	DET
fnp-5883	189	35	temperature	temperature	NOUN
fnp-5883	189	36	at	at	ADP
fnp-5883	189	37	which	which	PRON
fnp-5883	189	38	thermal	thermal	ADJ
fnp-5883	189	39	stress	stress	NOUN
fnp-5883	189	40	began	begin	VERB
fnp-5883	189	41	to	to	PART
fnp-5883	189	42	build	build	VERB
fnp-5883	189	43	up	up	ADP
fnp-5883	189	44	and	and	CCONJ
fnp-5883	189	45	fractures	fracture	NOUN
fnp-5883	189	46	began	begin	VERB
fnp-5883	189	47	to	to	PART
fnp-5883	189	48	occur	occur	VERB
fnp-5883	189	49	by	by	ADP
fnp-5883	189	50	only	only	ADV
fnp-5883	189	51	7.2	7.2	NUM
fnp-5883	189	52	 	 	SPACE
fnp-5883	189	53	°	°	PROPN
fnp-5883	189	54	c	c	NOUN
fnp-5883	189	55	(	(	PUNCT
fnp-5883	189	56	rabin	rabin	PROPN
fnp-5883	189	57	et	et	PROPN
fnp-5883	189	58	al	al	PROPN
fnp-5883	189	59	.	.	PROPN
fnp-5883	189	60	,	,	PUNCT
fnp-5883	189	61	2006	2006	NUM
fnp-5883	189	62	)	)	PUNCT
fnp-5883	189	63	.	.	PUNCT
fnp-5883	190	1	the	the	DET
fnp-5883	190	2	formation	formation	NOUN
fnp-5883	190	3	of	of	ADP
fnp-5883	190	4	brittle	brittle	ADJ
fnp-5883	190	5	ice	ice	NOUN
fnp-5883	190	6	crystals	crystal	NOUN
fnp-5883	190	7	in	in	ADP
fnp-5883	190	8	tissue	tissue	NOUN
fnp-5883	190	9	can	can	AUX
fnp-5883	190	10	not	not	PART
fnp-5883	190	11	only	only	ADV
fnp-5883	190	12	enhance	enhance	VERB
fnp-5883	190	13	fracturing	fracture	VERB
fnp-5883	190	14	during	during	ADP
fnp-5883	190	15	cooling	cooling	NOUN
fnp-5883	190	16	or	or	CCONJ
fnp-5883	190	17	storage	storage	NOUN
fnp-5883	190	18	,	,	PUNCT
fnp-5883	190	19	but	but	CCONJ
fnp-5883	190	20	also	also	ADV
fnp-5883	190	21	during	during	ADP
fnp-5883	190	22	subsequent	subsequent	ADJ
fnp-5883	190	23	sectioning	sectioning	NOUN
fnp-5883	190	24	,	,	PUNCT
fnp-5883	190	25	posing	pose	VERB
fnp-5883	190	26	a	a	DET
fnp-5883	190	27	challenge	challenge	NOUN
fnp-5883	190	28	for	for	ADP
fnp-5883	190	29	sample	sample	NOUN
fnp-5883	190	30	preparation	preparation	NOUN
fnp-5883	190	31	of	of	ADP
fnp-5883	190	32	cryopreserved	cryopreserved	ADJ
fnp-5883	190	33	tissue	tissue	NOUN
fnp-5883	190	34	(	(	PUNCT
fnp-5883	190	35	marshall	marshall	NOUN
fnp-5883	190	36	,	,	PUNCT
fnp-5883	190	37	1940	1940	NUM
fnp-5883	190	38	)	)	PUNCT
fnp-5883	190	39	.	.	PUNCT
fnp-5883	191	1	summary	summary	NOUN
fnp-5883	191	2	most	most	ADJ
fnp-5883	191	3	of	of	ADP
fnp-5883	191	4	the	the	DET
fnp-5883	191	5	structural	structural	ADJ
fnp-5883	191	6	damage	damage	NOUN
fnp-5883	191	7	that	that	PRON
fnp-5883	191	8	occurs	occur	VERB
fnp-5883	191	9	during	during	ADP
fnp-5883	191	10	cryopreservation	cryopreservation	NOUN
fnp-5883	191	11	of	of	ADP
fnp-5883	191	12	brain	brain	NOUN
fnp-5883	191	13	tissue	tissue	NOUN
fnp-5883	191	14	is	be	AUX
fnp-5883	191	15	related	relate	VERB
fnp-5883	191	16	to	to	ADP
fnp-5883	191	17	ice	ice	NOUN
fnp-5883	191	18	formation	formation	NOUN
fnp-5883	191	19	(	(	PUNCT
fnp-5883	191	20	table	table	NOUN
fnp-5883	191	21	 	 	SPACE
fnp-5883	191	22	1	1	NUM
fnp-5883	191	23	)	)	PUNCT
fnp-5883	191	24	.	.	PUNCT
fnp-5883	192	1	in	in	ADP
fnp-5883	192	2	addition	addition	NOUN
fnp-5883	192	3	,	,	PUNCT
fnp-5883	192	4	there	there	PRON
fnp-5883	192	5	are	be	VERB
fnp-5883	192	6	other	other	ADJ
fnp-5883	192	7	forms	form	NOUN
fnp-5883	192	8	of	of	ADP
fnp-5883	192	9	structural	structural	ADJ
fnp-5883	192	10	damage	damage	NOUN
fnp-5883	192	11	,	,	PUNCT
fnp-5883	192	12	such	such	ADJ
fnp-5883	192	13	as	as	ADP
fnp-5883	192	14	eutectic	eutectic	ADJ
fnp-5883	192	15	crystallization	crystallization	NOUN
fnp-5883	192	16	,	,	PUNCT
fnp-5883	192	17	cellular	cellular	ADJ
fnp-5883	192	18	decomposition	decomposition	NOUN
fnp-5883	192	19	,	,	PUNCT
fnp-5883	192	20	and	and	CCONJ
fnp-5883	192	21	fractures	fracture	NOUN
fnp-5883	192	22	that	that	PRON
fnp-5883	192	23	can	can	AUX
fnp-5883	192	24	be	be	AUX
fnp-5883	192	25	independent	independent	ADJ
fnp-5883	192	26	of	of	ADP
fnp-5883	192	27	ice	ice	NOUN
fnp-5883	192	28	formation	formation	NOUN
fnp-5883	192	29	.	.	PUNCT
fnp-5883	193	1	mechanism	mechanism	NOUN
fnp-5883	193	2	description	description	NOUN
fnp-5883	193	3	mechanical	mechanical	ADJ
fnp-5883	193	4	ice	ice	NOUN
fnp-5883	193	5	damage	damage	NOUN
fnp-5883	193	6	ice	ice	NOUN
fnp-5883	193	7	crystals	crystal	NOUN
fnp-5883	193	8	can	can	AUX
fnp-5883	193	9	cause	cause	VERB
fnp-5883	193	10	displacement	displacement	NOUN
fnp-5883	193	11	or	or	CCONJ
fnp-5883	193	12	rupture	rupture	NOUN
fnp-5883	193	13	of	of	ADP
fnp-5883	193	14	cell	cell	NOUN
fnp-5883	193	15	and	and	CCONJ
fnp-5883	193	16	organelle	organelle	NOUN
fnp-5883	193	17	membranes	membrane	NOUN
fnp-5883	193	18	cryosuction	cryosuction	NOUN
fnp-5883	193	19	water	water	NOUN
fnp-5883	193	20	drawn	draw	VERB
fnp-5883	193	21	towards	towards	ADP
fnp-5883	193	22	growing	grow	VERB
fnp-5883	193	23	ice	ice	NOUN
fnp-5883	193	24	crystals	crystal	NOUN
fnp-5883	193	25	causes	cause	VERB
fnp-5883	193	26	high	high	ADJ
fnp-5883	193	27	local	local	ADJ
fnp-5883	193	28	pressures	pressure	NOUN
fnp-5883	193	29	,	,	PUNCT
fnp-5883	193	30	leading	lead	VERB
fnp-5883	193	31	to	to	ADP
fnp-5883	193	32	potential	potential	ADJ
fnp-5883	193	33	alterations	alteration	NOUN
fnp-5883	193	34	of	of	ADP
fnp-5883	193	35	biomolecular	biomolecular	ADJ
fnp-5883	193	36	distributions	distribution	NOUN
fnp-5883	193	37	dehydration	dehydration	NOUN
fnp-5883	193	38	-	-	PUNCT
fnp-5883	193	39	associated	associate	VERB
fnp-5883	193	40	damage	damage	NOUN
fnp-5883	193	41	rapid	rapid	ADJ
fnp-5883	193	42	removal	removal	NOUN
fnp-5883	193	43	of	of	ADP
fnp-5883	193	44	water	water	NOUN
fnp-5883	193	45	,	,	PUNCT
fnp-5883	193	46	leads	lead	VERB
fnp-5883	193	47	to	to	ADP
fnp-5883	193	48	osmotic	osmotic	ADJ
fnp-5883	193	49	stress	stress	NOUN
fnp-5883	193	50	,	,	PUNCT
fnp-5883	193	51	cell	cell	NOUN
fnp-5883	193	52	dehydration	dehydration	NOUN
fnp-5883	193	53	,	,	PUNCT
fnp-5883	193	54	and	and	CCONJ
fnp-5883	193	55	potentially	potentially	ADV
fnp-5883	193	56	anisotropic	anisotropic	NOUN
fnp-5883	193	57	shrinkage	shrinkage	NOUN
fnp-5883	193	58	solution	solution	NOUN
fnp-5883	193	59	effects	effect	NOUN
fnp-5883	193	60	high	high	ADJ
fnp-5883	193	61	solute	solute	ADJ
fnp-5883	193	62	concentrations	concentration	NOUN
fnp-5883	193	63	during	during	ADP
fnp-5883	193	64	freezing	freezing	NOUN
fnp-5883	193	65	can	can	AUX
fnp-5883	193	66	cause	cause	VERB
fnp-5883	193	67	biomolecular	biomolecular	ADJ
fnp-5883	193	68	denaturation	denaturation	NOUN
fnp-5883	193	69	and	and	CCONJ
fnp-5883	193	70	aggregation	aggregation	NOUN
fnp-5883	193	71	,	,	PUNCT
fnp-5883	193	72	as	as	ADV
fnp-5883	193	73	well	well	ADV
fnp-5883	193	74	as	as	ADP
fnp-5883	193	75	other	other	ADJ
fnp-5883	193	76	forms	form	NOUN
fnp-5883	193	77	of	of	ADP
fnp-5883	193	78	damage	damage	NOUN
fnp-5883	193	79	cell	cell	NOUN
fnp-5883	193	80	membrane	membrane	NOUN
fnp-5883	193	81	permeabilization	permeabilization	NOUN
fnp-5883	193	82	if	if	SCONJ
fnp-5883	193	83	cell	cell	NOUN
fnp-5883	193	84	membranes	membrane	NOUN
fnp-5883	193	85	are	be	AUX
fnp-5883	193	86	damaged	damage	VERB
fnp-5883	193	87	during	during	ADP
fnp-5883	193	88	cryopreservation	cryopreservation	NOUN
fnp-5883	193	89	,	,	PUNCT
fnp-5883	193	90	they	they	PRON
fnp-5883	193	91	can	can	AUX
fnp-5883	193	92	be	be	AUX
fnp-5883	193	93	permeabilized	permeabilize	VERB
fnp-5883	193	94	,	,	PUNCT
fnp-5883	193	95	leaking	leak	VERB
fnp-5883	193	96	intracellular	intracellular	ADJ
fnp-5883	193	97	biomolecules	biomolecule	NOUN
fnp-5883	193	98	into	into	ADP
fnp-5883	193	99	the	the	DET
fnp-5883	193	100	local	local	ADJ
fnp-5883	193	101	area	area	NOUN
fnp-5883	193	102	cryoprotectant	cryoprotectant	NOUN
fnp-5883	193	103	toxicity	toxicity	NOUN
fnp-5883	193	104	cryoprotectants	cryoprotectant	NOUN
fnp-5883	193	105	,	,	PUNCT
fnp-5883	193	106	especially	especially	ADV
fnp-5883	193	107	if	if	SCONJ
fnp-5883	193	108	used	use	VERB
fnp-5883	193	109	at	at	ADP
fnp-5883	193	110	high	high	ADJ
fnp-5883	193	111	concentrations	concentration	NOUN
fnp-5883	193	112	and/or	and/or	CCONJ
fnp-5883	193	113	introduced	introduce	VERB
fnp-5883	193	114	rapidly	rapidly	ADV
fnp-5883	193	115	,	,	PUNCT
fnp-5883	193	116	can	can	AUX
fnp-5883	193	117	cause	cause	VERB
fnp-5883	193	118	osmotic	osmotic	ADJ
fnp-5883	193	119	stress	stress	NOUN
fnp-5883	193	120	and	and	CCONJ
fnp-5883	193	121	potentially	potentially	ADV
fnp-5883	193	122	other	other	ADJ
fnp-5883	193	123	forms	form	NOUN
fnp-5883	193	124	of	of	ADP
fnp-5883	193	125	toxicity	toxicity	NOUN
fnp-5883	193	126	eutectic	eutectic	ADJ
fnp-5883	193	127	crystallization	crystallization	NOUN
fnp-5883	193	128	formation	formation	NOUN
fnp-5883	193	129	of	of	ADP
fnp-5883	193	130	eutectic	eutectic	ADJ
fnp-5883	193	131	crystals	crystal	NOUN
fnp-5883	193	132	,	,	PUNCT
fnp-5883	193	133	such	such	ADJ
fnp-5883	193	134	as	as	ADP
fnp-5883	193	135	those	those	PRON
fnp-5883	193	136	formed	form	VERB
fnp-5883	193	137	by	by	ADP
fnp-5883	193	138	water	water	NOUN
fnp-5883	193	139	-	-	PUNCT
fnp-5883	193	140	sodium	sodium	NOUN
fnp-5883	193	141	chloride	chloride	NOUN
fnp-5883	193	142	,	,	PUNCT
fnp-5883	193	143	can	can	AUX
fnp-5883	193	144	cause	cause	VERB
fnp-5883	193	145	damage	damage	NOUN
fnp-5883	193	146	to	to	ADP
fnp-5883	193	147	cellular	cellular	ADJ
fnp-5883	193	148	structures	structure	NOUN
fnp-5883	193	149	cellular	cellular	ADJ
fnp-5883	193	150	decomposition	decomposition	NOUN
fnp-5883	193	151	regions	region	NOUN
fnp-5883	193	152	of	of	ADP
fnp-5883	193	153	the	the	DET
fnp-5883	193	154	tissue	tissue	NOUN
fnp-5883	193	155	that	that	PRON
fnp-5883	193	156	have	have	AUX
fnp-5883	193	157	not	not	PART
fnp-5883	193	158	yet	yet	ADV
fnp-5883	193	159	cooled	cool	VERB
fnp-5883	193	160	to	to	ADP
fnp-5883	193	161	sufficiently	sufficiently	ADV
fnp-5883	193	162	low	low	ADJ
fnp-5883	193	163	temperatures	temperature	NOUN
fnp-5883	193	164	may	may	AUX
fnp-5883	193	165	undergo	undergo	VERB
fnp-5883	193	166	autolysis	autolysis	NOUN
fnp-5883	193	167	or	or	CCONJ
fnp-5883	193	168	putrefaction	putrefaction	NOUN
fnp-5883	193	169	fractures	fracture	VERB
fnp-5883	193	170	susceptibility	susceptibility	NOUN
fnp-5883	193	171	to	to	ADP
fnp-5883	193	172	fractures	fracture	NOUN
fnp-5883	193	173	during	during	ADP
fnp-5883	193	174	cooling	cool	VERB
fnp-5883	193	175	and	and	CCONJ
fnp-5883	193	176	storage	storage	NOUN
fnp-5883	193	177	table	table	NOUN
fnp-5883	193	178	 	 	SPACE
fnp-5883	193	179	1	1	NUM
fnp-5883	193	180	.	.	PUNCT
fnp-5883	194	1	summary	summary	NOUN
fnp-5883	194	2	of	of	ADP
fnp-5883	194	3	mechanisms	mechanism	NOUN
fnp-5883	194	4	of	of	ADP
fnp-5883	194	5	damage	damage	NOUN
fnp-5883	194	6	during	during	ADP
fnp-5883	194	7	cryopreservation	cryopreservation	NOUN
fnp-5883	194	8	of	of	ADP
fnp-5883	194	9	brain	brain	NOUN
fnp-5883	194	10	tissue	tissue	NOUN
fnp-5883	194	11	.	.	PUNCT
fnp-5883	195	1	cryofixation	cryofixation	NOUN
fnp-5883	195	2	preservation	preservation	NOUN
fnp-5883	195	3	process	process	NOUN
fnp-5883	195	4	cryofixation	cryofixation	NOUN
fnp-5883	195	5	refers	refer	VERB
fnp-5883	195	6	to	to	ADP
fnp-5883	195	7	the	the	DET
fnp-5883	195	8	ultrarapid	ultrarapid	NOUN
fnp-5883	195	9	cooling	cooling	NOUN
fnp-5883	195	10	of	of	ADP
fnp-5883	195	11	tissue	tissue	NOUN
fnp-5883	195	12	that	that	PRON
fnp-5883	195	13	is	be	AUX
fnp-5883	195	14	fast	fast	ADJ
fnp-5883	195	15	enough	enough	ADV
fnp-5883	195	16	to	to	PART
fnp-5883	195	17	prevent	prevent	VERB
fnp-5883	195	18	ice	ice	NOUN
fnp-5883	195	19	formation	formation	NOUN
fnp-5883	195	20	during	during	ADP
fnp-5883	195	21	cryopreservation	cryopreservation	NOUN
fnp-5883	195	22	(	(	PUNCT
fnp-5883	195	23	gilkey	gilkey	NOUN
fnp-5883	195	24	and	and	CCONJ
fnp-5883	195	25	staehelin	staehelin	NOUN
fnp-5883	195	26	,	,	PUNCT
fnp-5883	195	27	1986	1986	NUM
fnp-5883	195	28	)	)	PUNCT
fnp-5883	195	29	.	.	PUNCT
fnp-5883	196	1	major	major	ADJ
fnp-5883	196	2	types	type	NOUN
fnp-5883	196	3	include	include	VERB
fnp-5883	196	4	(	(	PUNCT
fnp-5883	196	5	a	a	X
fnp-5883	196	6	)	)	PUNCT
fnp-5883	196	7	plunge	plunge	NOUN
fnp-5883	196	8	freezing	freezing	NOUN
fnp-5883	196	9	,	,	PUNCT
fnp-5883	196	10	which	which	PRON
fnp-5883	196	11	directly	directly	ADV
fnp-5883	196	12	immerses	immerse	VERB
fnp-5883	196	13	a	a	DET
fnp-5883	196	14	sample	sample	NOUN
fnp-5883	196	15	into	into	ADP
fnp-5883	196	16	a	a	DET
fnp-5883	196	17	liquid	liquid	ADJ
fnp-5883	196	18	cryogen	cryogen	NOUN
fnp-5883	196	19	,	,	PUNCT
fnp-5883	196	20	(	(	PUNCT
fnp-5883	196	21	b	b	NOUN
fnp-5883	196	22	)	)	PUNCT
fnp-5883	196	23	propane	propane	NOUN
fnp-5883	196	24	jet	jet	NOUN
fnp-5883	196	25	freezing	freezing	NOUN
fnp-5883	196	26	,	,	PUNCT
fnp-5883	196	27	which	which	PRON
fnp-5883	196	28	sprays	spray	VERB
fnp-5883	196	29	a	a	DET
fnp-5883	196	30	liquid	liquid	ADJ
fnp-5883	196	31	cryogen	cryogen	NOUN
fnp-5883	196	32	onto	onto	ADP
fnp-5883	196	33	a	a	DET
fnp-5883	196	34	sample	sample	NOUN
fnp-5883	196	35	,	,	PUNCT
fnp-5883	196	36	(	(	PUNCT
fnp-5883	196	37	c	c	X
fnp-5883	196	38	)	)	PUNCT
fnp-5883	196	39	cold	cold	ADJ
fnp-5883	196	40	metal	metal	NOUN
fnp-5883	196	41	block	block	NOUN
fnp-5883	196	42	freezing	freezing	NOUN
fnp-5883	196	43	,	,	PUNCT
fnp-5883	196	44	which	which	PRON
fnp-5883	196	45	rapidly	rapidly	ADV
fnp-5883	196	46	brings	bring	VERB
fnp-5883	196	47	the	the	DET
fnp-5883	196	48	sample	sample	NOUN
fnp-5883	196	49	into	into	ADP
fnp-5883	196	50	contact	contact	NOUN
fnp-5883	196	51	with	with	ADP
fnp-5883	196	52	a	a	DET
fnp-5883	196	53	pre	pre	ADJ
fnp-5883	196	54	-	-	ADJ
fnp-5883	196	55	cooled	cooled	ADJ
fnp-5883	196	56	metal	metal	NOUN
fnp-5883	196	57	surface	surface	NOUN
fnp-5883	196	58	,	,	PUNCT
fnp-5883	196	59	and	and	CCONJ
fnp-5883	196	60	(	(	PUNCT
fnp-5883	196	61	d	d	NOUN
fnp-5883	196	62	)	)	PUNCT
fnp-5883	196	63	high	high	ADJ
fnp-5883	196	64	-	-	PUNCT
fnp-5883	196	65	pressure	pressure	NOUN
fnp-5883	196	66	freezing	freezing	NOUN
fnp-5883	196	67	,	,	PUNCT
fnp-5883	196	68	which	which	PRON
fnp-5883	196	69	performs	perform	VERB
fnp-5883	196	70	the	the	DET
fnp-5883	196	71	cooling	cooling	NOUN
fnp-5883	196	72	in	in	ADP
fnp-5883	196	73	a	a	DET
fnp-5883	196	74	high	high	ADJ
fnp-5883	196	75	pressure	pressure	NOUN
fnp-5883	196	76	system	system	NOUN
fnp-5883	196	77	to	to	PART
fnp-5883	196	78	help	help	VERB
fnp-5883	196	79	suppress	suppress	VERB
fnp-5883	196	80	ice	ice	NOUN
fnp-5883	196	81	formation	formation	NOUN
fnp-5883	196	82	(	(	PUNCT
fnp-5883	196	83	gilkey	gilkey	NOUN
fnp-5883	196	84	and	and	CCONJ
fnp-5883	196	85	staehelin	staehelin	NOUN
fnp-5883	196	86	,	,	PUNCT
fnp-5883	196	87	1986	1986	NUM
fnp-5883	196	88	)	)	PUNCT
fnp-5883	196	89	.	.	PUNCT
fnp-5883	197	1	one	one	NUM
fnp-5883	197	2	source	source	NOUN
fnp-5883	197	3	reports	report	VERB
fnp-5883	197	4	that	that	SCONJ
fnp-5883	197	5	,	,	PUNCT
fnp-5883	197	6	in	in	ADP
fnp-5883	197	7	tissues	tissue	NOUN
fnp-5883	197	8	without	without	ADP
fnp-5883	197	9	any	any	DET
fnp-5883	197	10	cryoprotectants	cryoprotectant	NOUN
fnp-5883	197	11	added	add	VERB
fnp-5883	197	12	,	,	PUNCT
fnp-5883	197	13	vitrification	vitrification	NOUN
fnp-5883	197	14	will	will	AUX
fnp-5883	197	15	result	result	VERB
fnp-5883	197	16	when	when	SCONJ
fnp-5883	197	17	it	it	PRON
fnp-5883	197	18	is	be	AUX
fnp-5883	197	19	cooled	cool	VERB
fnp-5883	197	20	at	at	ADP
fnp-5883	197	21	a	a	DET
fnp-5883	197	22	rate	rate	NOUN
fnp-5883	197	23	faster	fast	ADJ
fnp-5883	197	24	than	than	ADP
fnp-5883	197	25	200,000	200,000	NUM
fnp-5883	197	26	 	 	SPACE
fnp-5883	197	27	k	k	PROPN
fnp-5883	197	28	/	/	SYM
fnp-5883	197	29	s	s	X
fnp-5883	197	30	(	(	PUNCT
fnp-5883	197	31	studer	studer	NOUN
fnp-5883	197	32	et	et	NOUN
fnp-5883	197	33	al	al	PROPN
fnp-5883	197	34	.	.	PROPN
fnp-5883	197	35	,	,	PUNCT
fnp-5883	197	36	2014	2014	NUM
fnp-5883	197	37	)	)	PUNCT
fnp-5883	197	38	.	.	PUNCT
fnp-5883	198	1	by	by	ADP
fnp-5883	198	2	increasing	increase	VERB
fnp-5883	198	3	the	the	DET
fnp-5883	198	4	pressure	pressure	NOUN
fnp-5883	198	5	to	to	ADP
fnp-5883	198	6	around	around	ADP
fnp-5883	198	7	2000	2000	NUM
fnp-5883	198	8	 	 	SPACE
fnp-5883	198	9	bar	bar	NOUN
fnp-5883	198	10	,	,	PUNCT
fnp-5883	198	11	high	high	ADJ
fnp-5883	198	12	-	-	PUNCT
fnp-5883	198	13	pressure	pressure	NOUN
fnp-5883	198	14	freezing	freezing	NOUN
fnp-5883	198	15	lowers	lower	VERB
fnp-5883	198	16	the	the	DET
fnp-5883	198	17	freezing	freezing	ADJ
fnp-5883	198	18	point	point	NOUN
fnp-5883	198	19	of	of	ADP
fnp-5883	198	20	water	water	NOUN
fnp-5883	198	21	to	to	ADP
fnp-5883	198	22	‑22	‑22	NOUN
fnp-5883	198	23	 	 	SPACE
fnp-5883	198	24	°	°	ADP
fnp-5883	198	25	c	c	NOUN
fnp-5883	198	26	,	,	PUNCT
fnp-5883	198	27	which	which	PRON
fnp-5883	198	28	reduces	reduce	VERB
fnp-5883	198	29	the	the	DET
fnp-5883	198	30	critical	critical	ADJ
fnp-5883	198	31	cooling	cool	VERB
fnp-5883	198	32	rate	rate	NOUN
fnp-5883	198	33	for	for	ADP
fnp-5883	198	34	ice	ice	NOUN
fnp-5883	198	35	formation	formation	NOUN
fnp-5883	198	36	by	by	ADP
fnp-5883	198	37	a	a	DET
fnp-5883	198	38	factor	factor	NOUN
fnp-5883	198	39	of	of	ADP
fnp-5883	198	40	100	100	NUM
fnp-5883	198	41	(	(	PUNCT
fnp-5883	198	42	studer	studer	NOUN
fnp-5883	198	43	et	et	NOUN
fnp-5883	198	44	al	al	PROPN
fnp-5883	198	45	.	.	PROPN
fnp-5883	198	46	,	,	PUNCT
fnp-5883	198	47	2014	2014	NUM
fnp-5883	198	48	)	)	PUNCT
fnp-5883	198	49	.	.	PUNCT
fnp-5883	199	1	the	the	DET
fnp-5883	199	2	vitrification	vitrification	NOUN
fnp-5883	199	3	depth	depth	NOUN
fnp-5883	199	4	of	of	ADP
fnp-5883	199	5	a	a	DET
fnp-5883	199	6	cryofixation	cryofixation	NOUN
fnp-5883	199	7	procedure	procedure	NOUN
fnp-5883	199	8	is	be	AUX
fnp-5883	199	9	the	the	DET
fnp-5883	199	10	distance	distance	NOUN
fnp-5883	199	11	from	from	ADP
fnp-5883	199	12	the	the	DET
fnp-5883	199	13	surface	surface	NOUN
fnp-5883	199	14	of	of	ADP
fnp-5883	199	15	the	the	DET
fnp-5883	199	16	tissue	tissue	NOUN
fnp-5883	199	17	that	that	PRON
fnp-5883	199	18	can	can	AUX
fnp-5883	199	19	be	be	AUX
fnp-5883	199	20	vitrified	vitrify	VERB
fnp-5883	199	21	.	.	PUNCT
fnp-5883	200	1	with	with	ADP
fnp-5883	200	2	the	the	DET
fnp-5883	200	3	use	use	NOUN
fnp-5883	200	4	of	of	ADP
fnp-5883	200	5	plunge	plunge	NOUN
fnp-5883	200	6	freezing	freezing	NOUN
fnp-5883	200	7	or	or	CCONJ
fnp-5883	200	8	cold	cold	ADJ
fnp-5883	200	9	metal	metal	NOUN
fnp-5883	200	10	block	block	NOUN
fnp-5883	200	11	freezing	freezing	NOUN
fnp-5883	200	12	,	,	PUNCT
fnp-5883	200	13	the	the	DET
fnp-5883	200	14	vitrification	vitrification	NOUN
fnp-5883	200	15	depth	depth	NOUN
fnp-5883	200	16	is	be	AUX
fnp-5883	200	17	usually	usually	ADV
fnp-5883	200	18	approximately	approximately	ADV
fnp-5883	200	19	10–20	10–20	NUM
fnp-5883	200	20	 	 	SPACE
fnp-5883	200	21	µm	µm	NOUN
fnp-5883	200	22	,	,	PUNCT
fnp-5883	200	23	but	but	CCONJ
fnp-5883	200	24	this	this	PRON
fnp-5883	200	25	can	can	AUX
fnp-5883	200	26	be	be	AUX
fnp-5883	200	27	increased	increase	VERB
fnp-5883	200	28	10	10	NUM
fnp-5883	200	29	-	-	ADJ
fnp-5883	200	30	fold	fold	ADJ
fnp-5883	200	31	to	to	ADP
fnp-5883	200	32	samples	sample	NOUN
fnp-5883	200	33	around	around	ADP
fnp-5883	200	34	200	200	NUM
fnp-5883	200	35	 	 	SPACE
fnp-5883	200	36	µm	µm	NOUN
fnp-5883	200	37	thick	thick	ADJ
fnp-5883	200	38	with	with	ADP
fnp-5883	200	39	the	the	DET
fnp-5883	200	40	use	use	NOUN
fnp-5883	200	41	of	of	ADP
fnp-5883	200	42	high	high	ADJ
fnp-5883	200	43	-	-	PUNCT
fnp-5883	200	44	pressure	pressure	NOUN
fnp-5883	200	45	freezing	freezing	NOUN
fnp-5883	200	46	(	(	PUNCT
fnp-5883	200	47	studer	studer	NOUN
fnp-5883	200	48	et	et	NOUN
fnp-5883	200	49	al	al	PROPN
fnp-5883	200	50	.	.	PROPN
fnp-5883	200	51	,	,	PUNCT
fnp-5883	200	52	2014	2014	NUM
fnp-5883	200	53	)	)	PUNCT
fnp-5883	200	54	.	.	PUNCT
fnp-5883	201	1	while	while	SCONJ
fnp-5883	201	2	increased	increase	VERB
fnp-5883	201	3	pressure	pressure	NOUN
fnp-5883	201	4	to	to	ADP
fnp-5883	201	5	this	this	DET
fnp-5883	201	6	degree	degree	NOUN
fnp-5883	201	7	can	can	AUX
fnp-5883	201	8	be	be	AUX
fnp-5883	201	9	lethal	lethal	ADJ
fnp-5883	201	10	,	,	PUNCT
fnp-5883	201	11	it	it	PRON
fnp-5883	201	12	can	can	AUX
fnp-5883	201	13	be	be	AUX
fnp-5883	201	14	kept	keep	VERB
fnp-5883	201	15	to	to	ADP
fnp-5883	201	16	a	a	DET
fnp-5883	201	17	very	very	ADV
fnp-5883	201	18	short	short	ADJ
fnp-5883	201	19	period	period	NOUN
fnp-5883	201	20	of	of	ADP
fnp-5883	201	21	less	less	ADJ
fnp-5883	201	22	than	than	ADP
fnp-5883	201	23	0.1	0.1	NUM
fnp-5883	201	24	seconds	second	NOUN
fnp-5883	201	25	during	during	ADP
fnp-5883	201	26	the	the	DET
fnp-5883	201	27	high	high	ADJ
fnp-5883	201	28	-	-	PUNCT
fnp-5883	201	29	pressure	pressure	NOUN
fnp-5883	201	30	freezing	freezing	NOUN
fnp-5883	201	31	procedure	procedure	NOUN
fnp-5883	201	32	(	(	PUNCT
fnp-5883	201	33	moor	moor	PROPN
fnp-5883	201	34	et	et	PROPN
fnp-5883	201	35	al	al	PROPN
fnp-5883	201	36	.	.	PROPN
fnp-5883	201	37	,	,	PUNCT
fnp-5883	201	38	1980	1980	NUM
fnp-5883	201	39	)	)	PUNCT
fnp-5883	201	40	.	.	PUNCT
fnp-5883	202	1	as	as	ADP
fnp-5883	202	2	a	a	DET
fnp-5883	202	3	result	result	NOUN
fnp-5883	202	4	,	,	PUNCT
fnp-5883	202	5	the	the	DET
fnp-5883	202	6	increased	increase	VERB
fnp-5883	202	7	pressure	pressure	NOUN
fnp-5883	202	8	is	be	AUX
fnp-5883	202	9	expected	expect	VERB
fnp-5883	202	10	to	to	PART
fnp-5883	202	11	have	have	VERB
fnp-5883	202	12	minimal	minimal	ADJ
fnp-5883	202	13	effect	effect	NOUN
fnp-5883	202	14	on	on	ADP
fnp-5883	202	15	the	the	DET
fnp-5883	202	16	tissue	tissue	NOUN
fnp-5883	202	17	,	,	PUNCT
fnp-5883	202	18	although	although	SCONJ
fnp-5883	202	19	it	it	PRON
fnp-5883	202	20	has	have	AUX
fnp-5883	202	21	been	be	AUX
fnp-5883	202	22	reported	report	VERB
fnp-5883	202	23	to	to	PART
fnp-5883	202	24	affect	affect	VERB
fnp-5883	202	25	the	the	DET
fnp-5883	202	26	structure	structure	NOUN
fnp-5883	202	27	of	of	ADP
fnp-5883	202	28	some	some	DET
fnp-5883	202	29	biomolecules	biomolecule	NOUN
fnp-5883	202	30	(	(	PUNCT
fnp-5883	202	31	vanhecke	vanhecke	NOUN
fnp-5883	202	32	et	et	PROPN
fnp-5883	202	33	al	al	PROPN
fnp-5883	202	34	.	.	PROPN
fnp-5883	202	35	,	,	PUNCT
fnp-5883	202	36	2010	2010	NUM
fnp-5883	202	37	)	)	PUNCT
fnp-5883	202	38	.	.	PUNCT
fnp-5883	203	1	occasionally	occasionally	ADV
fnp-5883	203	2	,	,	PUNCT
fnp-5883	203	3	cryoprotectants	cryoprotectant	NOUN
fnp-5883	203	4	are	be	AUX
fnp-5883	203	5	also	also	ADV
fnp-5883	203	6	used	use	VERB
fnp-5883	203	7	to	to	PART
fnp-5883	203	8	aid	aid	VERB
fnp-5883	203	9	in	in	ADP
fnp-5883	203	10	the	the	DET
fnp-5883	203	11	process	process	NOUN
fnp-5883	203	12	of	of	ADP
fnp-5883	203	13	cryofixation	cryofixation	NOUN
fnp-5883	203	14	.	.	PUNCT
fnp-5883	204	1	this	this	PRON
fnp-5883	204	2	helps	help	VERB
fnp-5883	204	3	to	to	PART
fnp-5883	204	4	prevent	prevent	VERB
fnp-5883	204	5	ice	ice	NOUN
fnp-5883	204	6	damage	damage	NOUN
fnp-5883	204	7	,	,	PUNCT
fnp-5883	204	8	but	but	CCONJ
fnp-5883	204	9	can	can	AUX
fnp-5883	204	10	lead	lead	VERB
fnp-5883	204	11	to	to	ADP
fnp-5883	204	12	structural	structural	ADJ
fnp-5883	204	13	alterations	alteration	NOUN
fnp-5883	204	14	of	of	ADP
fnp-5883	204	15	their	their	PRON
fnp-5883	204	16	own	own	ADJ
fnp-5883	204	17	.	.	PUNCT
fnp-5883	205	1	as	as	ADP
fnp-5883	205	2	a	a	DET
fnp-5883	205	3	result	result	NOUN
fnp-5883	205	4	,	,	PUNCT
fnp-5883	205	5	cryoprotectants	cryoprotectant	NOUN
fnp-5883	205	6	are	be	AUX
fnp-5883	205	7	only	only	ADV
fnp-5883	205	8	sometimes	sometimes	ADV
fnp-5883	205	9	used	use	VERB
fnp-5883	205	10	in	in	ADP
fnp-5883	205	11	cryofixation	cryofixation	NOUN
fnp-5883	205	12	,	,	PUNCT
fnp-5883	205	13	depending	depend	VERB
fnp-5883	205	14	on	on	ADP
fnp-5883	205	15	the	the	DET
fnp-5883	205	16	goals	goal	NOUN
fnp-5883	205	17	of	of	ADP
fnp-5883	205	18	the	the	DET
fnp-5883	205	19	experiment	experiment	NOUN
fnp-5883	205	20	.	.	PUNCT
fnp-5883	206	1	notably	notably	ADV
fnp-5883	206	2	,	,	PUNCT
fnp-5883	206	3	potential	potential	ADJ
fnp-5883	206	4	damage	damage	NOUN
fnp-5883	206	5	during	during	ADP
fnp-5883	206	6	rewarming	rewarming	NOUN
fnp-5883	206	7	is	be	AUX
fnp-5883	206	8	often	often	ADV
fnp-5883	206	9	avoided	avoid	VERB
fnp-5883	206	10	entirely	entirely	ADV
fnp-5883	206	11	for	for	ADP
fnp-5883	206	12	cryofixation	cryofixation	NOUN
fnp-5883	206	13	studies	study	NOUN
fnp-5883	206	14	because	because	SCONJ
fnp-5883	206	15	investigators	investigator	NOUN
fnp-5883	206	16	frequently	frequently	ADV
fnp-5883	206	17	use	use	VERB
fnp-5883	206	18	freeze	freeze	NOUN
fnp-5883	206	19	substitution	substitution	NOUN
fnp-5883	206	20	,	,	PUNCT
fnp-5883	206	21	or	or	CCONJ
fnp-5883	206	22	less	less	ADV
fnp-5883	206	23	commonly	commonly	ADV
fnp-5883	206	24	freeze	freeze	VERB
fnp-5883	206	25	fracture	fracture	NOUN
fnp-5883	206	26	,	,	PUNCT
fnp-5883	206	27	to	to	PART
fnp-5883	206	28	avoid	avoid	VERB
fnp-5883	206	29	this	this	DET
fnp-5883	206	30	issue	issue	NOUN
fnp-5883	206	31	.	.	PUNCT
fnp-5883	207	1	effects	effect	NOUN
fnp-5883	207	2	on	on	ADP
fnp-5883	207	3	tissue	tissue	NOUN
fnp-5883	207	4	morphology	morphology	NOUN
fnp-5883	207	5	the	the	DET
fnp-5883	207	6	majority	majority	NOUN
fnp-5883	207	7	of	of	ADP
fnp-5883	207	8	studies	study	NOUN
fnp-5883	207	9	we	we	PRON
fnp-5883	207	10	identified	identify	VERB
fnp-5883	207	11	found	find	VERB
fnp-5883	207	12	that	that	SCONJ
fnp-5883	207	13	cryofixation	cryofixation	NOUN
fnp-5883	207	14	,	,	PUNCT
fnp-5883	207	15	and	and	CCONJ
fnp-5883	207	16	especially	especially	ADV
fnp-5883	207	17	high	high	ADJ
fnp-5883	207	18	-	-	PUNCT
fnp-5883	207	19	pressure	pressure	NOUN
fnp-5883	207	20	freezing	freezing	NOUN
fnp-5883	207	21	,	,	PUNCT
fnp-5883	207	22	can	can	AUX
fnp-5883	207	23	enable	enable	VERB
fnp-5883	207	24	pristine	pristine	ADJ
fnp-5883	207	25	ultrastructural	ultrastructural	ADJ
fnp-5883	207	26	preservation	preservation	NOUN
fnp-5883	207	27	of	of	ADP
fnp-5883	207	28	brain	brain	NOUN
fnp-5883	207	29	tissue	tissue	NOUN
fnp-5883	207	30	in	in	ADP
fnp-5883	207	31	the	the	DET
fnp-5883	207	32	areas	area	NOUN
fnp-5883	207	33	that	that	PRON
fnp-5883	207	34	are	be	AUX
fnp-5883	207	35	vitrified	vitrify	VERB
fnp-5883	207	36	(	(	PUNCT
fnp-5883	207	37	table	table	NOUN
fnp-5883	207	38	 	 	SPACE
fnp-5883	207	39	2	2	NUM
fnp-5883	207	40	;	;	PUNCT
fnp-5883	207	41	supplementary	supplementary	ADJ
fnp-5883	207	42	file	file	NOUN
fnp-5883	207	43	 	 	SPACE
fnp-5883	207	44	4	4	NUM
fnp-5883	207	45	)	)	PUNCT
fnp-5883	207	46	(	(	PUNCT
fnp-5883	207	47	benson	benson	PROPN
fnp-5883	207	48	et	et	PROPN
fnp-5883	207	49	al	al	PROPN
fnp-5883	207	50	.	.	PROPN
fnp-5883	207	51	,	,	PUNCT
fnp-5883	207	52	1985	1985	NUM
fnp-5883	207	53	;	;	PUNCT
fnp-5883	207	54	guo	guo	PROPN
fnp-5883	207	55	et	et	PROPN
fnp-5883	207	56	al	al	PROPN
fnp-5883	207	57	.	.	PROPN
fnp-5883	207	58	,	,	PUNCT
fnp-5883	207	59	2020	2020	NUM
fnp-5883	207	60	)	)	PUNCT
fnp-5883	207	61	.	.	PUNCT
fnp-5883	208	1	cryofixation	cryofixation	NOUN
fnp-5883	208	2	preserves	preserve	VERB
fnp-5883	208	3	membranes	membrane	NOUN
fnp-5883	208	4	,	,	PUNCT
fnp-5883	208	5	organelles	organelle	NOUN
fnp-5883	208	6	,	,	PUNCT
fnp-5883	208	7	synapses	synapsis	NOUN
fnp-5883	208	8	,	,	PUNCT
fnp-5883	208	9	and	and	CCONJ
fnp-5883	208	10	other	other	ADJ
fnp-5883	208	11	neural	neural	ADJ
fnp-5883	208	12	structures	structure	NOUN
fnp-5883	208	13	with	with	ADP
fnp-5883	208	14	life	life	NOUN
fnp-5883	208	15	-	-	PUNCT
fnp-5883	208	16	like	like	ADJ
fnp-5883	208	17	morphology	morphology	NOUN
fnp-5883	208	18	.	.	PUNCT
fnp-5883	209	1	indeed	indeed	ADV
fnp-5883	209	2	,	,	PUNCT
fnp-5883	209	3	vitrification	vitrification	NOUN
fnp-5883	209	4	by	by	ADP
fnp-5883	209	5	cryofixation	cryofixation	NOUN
fnp-5883	209	6	is	be	AUX
fnp-5883	209	7	considered	consider	VERB
fnp-5883	209	8	the	the	DET
fnp-5883	209	9	best	good	ADJ
fnp-5883	209	10	method	method	NOUN
fnp-5883	209	11	for	for	ADP
fnp-5883	209	12	structural	structural	ADJ
fnp-5883	209	13	preservation	preservation	NOUN
fnp-5883	209	14	,	,	PUNCT
fnp-5883	209	15	superior	superior	ADJ
fnp-5883	209	16	to	to	ADP
fnp-5883	209	17	chemical	chemical	NOUN
fnp-5883	209	18	fixation	fixation	NOUN
fnp-5883	209	19	,	,	PUNCT
fnp-5883	209	20	because	because	SCONJ
fnp-5883	209	21	it	it	PRON
fnp-5883	209	22	can	can	AUX
fnp-5883	209	23	preserve	preserve	VERB
fnp-5883	209	24	tissue	tissue	NOUN
fnp-5883	209	25	within	within	ADP
fnp-5883	209	26	milliseconds	millisecond	NOUN
fnp-5883	209	27	,	,	PUNCT
fnp-5883	209	28	rather	rather	ADV
fnp-5883	209	29	than	than	ADP
fnp-5883	209	30	chemical	chemical	ADJ
fnp-5883	209	31	fixation	fixation	NOUN
fnp-5883	209	32	,	,	PUNCT
fnp-5883	209	33	which	which	PRON
fnp-5883	209	34	takes	take	VERB
fnp-5883	209	35	at	at	ADV
fnp-5883	209	36	least	least	ADJ
fnp-5883	209	37	seconds	second	NOUN
fnp-5883	209	38	to	to	ADP
fnp-5883	209	39	minutes	minute	NOUN
fnp-5883	209	40	(	(	PUNCT
fnp-5883	209	41	sosinsky	sosinsky	PROPN
fnp-5883	209	42	et	et	PROPN
fnp-5883	209	43	al	al	PROPN
fnp-5883	209	44	.	.	PROPN
fnp-5883	209	45	,	,	PUNCT
fnp-5883	209	46	2008	2008	NUM
fnp-5883	209	47	)	)	PUNCT
fnp-5883	209	48	.	.	PUNCT
fnp-5883	210	1	sample	sample	NOUN
fnp-5883	210	2	type	type	NOUN
fnp-5883	210	3	(	(	PUNCT
fnp-5883	210	4	study	study	NOUN
fnp-5883	210	5	)	)	PUNCT
fnp-5883	210	6	cooling	cool	VERB
fnp-5883	210	7	method	method	NOUN
fnp-5883	210	8	visualization	visualization	NOUN
fnp-5883	210	9	method	method	NOUN
fnp-5883	210	10	key	key	ADJ
fnp-5883	210	11	findings	finding	NOUN
fnp-5883	210	12	slabs	slab	NOUN
fnp-5883	210	13	of	of	ADP
fnp-5883	210	14	mouse	mouse	NOUN
fnp-5883	210	15	cerebellar	cerebellar	ADJ
fnp-5883	210	16	cortex	cortex	NOUN
fnp-5883	210	17	(	(	PUNCT
fnp-5883	210	18	heuser	heuser	X
fnp-5883	210	19	et	et	PROPN
fnp-5883	210	20	al	al	PROPN
fnp-5883	210	21	.	.	PROPN
fnp-5883	210	22	,	,	PUNCT
fnp-5883	210	23	1976	1976	NUM
fnp-5883	210	24	)	)	PUNCT
fnp-5883	210	25	pressing	press	VERB
fnp-5883	210	26	tissue	tissue	NOUN
fnp-5883	210	27	against	against	ADP
fnp-5883	210	28	copper	copper	NOUN
fnp-5883	210	29	block	block	NOUN
fnp-5883	210	30	cooled	cool	VERB
fnp-5883	210	31	to	to	ADP
fnp-5883	210	32	liquid	liquid	ADJ
fnp-5883	210	33	helium	helium	NOUN
fnp-5883	210	34	temperature	temperature	NOUN
fnp-5883	210	35	freeze	freeze	NOUN
fnp-5883	210	36	-	-	PUNCT
fnp-5883	210	37	fracture	fracture	NOUN
fnp-5883	210	38	electron	electron	NOUN
fnp-5883	210	39	microscopy	microscopy	NOUN
fnp-5883	210	40	first	first	ADV
fnp-5883	210	41	20	20	NUM
fnp-5883	210	42	 	 	SPACE
fnp-5883	210	43	µm	µm	NOUN
fnp-5883	210	44	has	have	VERB
fnp-5883	210	45	good	good	ADJ
fnp-5883	210	46	ultrastructure	ultrastructure	NOUN
fnp-5883	210	47	;	;	PUNCT
fnp-5883	210	48	deeper	deep	ADJ
fnp-5883	210	49	than	than	ADP
fnp-5883	210	50	20	20	NUM
fnp-5883	210	51	 	 	SPACE
fnp-5883	210	52	µm	µm	NOUN
fnp-5883	210	53	,	,	PUNCT
fnp-5883	210	54	cell	cell	NOUN
fnp-5883	210	55	processes	process	NOUN
fnp-5883	210	56	are	be	AUX
fnp-5883	210	57	distorted	distort	VERB
fnp-5883	210	58	and	and	CCONJ
fnp-5883	210	59	large	large	ADJ
fnp-5883	210	60	ice	ice	NOUN
fnp-5883	210	61	crystals	crystal	NOUN
fnp-5883	210	62	appear	appear	VERB
fnp-5883	210	63	present	present	ADJ
fnp-5883	210	64	3	3	NUM
fnp-5883	210	65	 	 	SPACE
fnp-5883	210	66	mm	mm	NUM
fnp-5883	210	67	mouse	mouse	NOUN
fnp-5883	210	68	cerebellar	cerebellar	PROPN
fnp-5883	210	69	hemisphere	hemisphere	ADJ
fnp-5883	210	70	slices	slice	NOUN
fnp-5883	210	71	(	(	PUNCT
fnp-5883	210	72	landis	landis	PROPN
fnp-5883	210	73	and	and	CCONJ
fnp-5883	210	74	reese	reese	PROPN
fnp-5883	210	75	,	,	PUNCT
fnp-5883	210	76	1983	1983	NUM
fnp-5883	210	77	)	)	PUNCT
fnp-5883	210	78	freezing	freeze	VERB
fnp-5883	210	79	with	with	ADP
fnp-5883	210	80	a	a	DET
fnp-5883	210	81	copper	copper	NOUN
fnp-5883	210	82	block	block	NOUN
fnp-5883	210	83	cooled	cool	VERB
fnp-5883	210	84	with	with	ADP
fnp-5883	210	85	liquid	liquid	ADJ
fnp-5883	210	86	helium	helium	NOUN
fnp-5883	210	87	freeze	freeze	NOUN
fnp-5883	210	88	substitution	substitution	NOUN
fnp-5883	210	89	,	,	PUNCT
fnp-5883	210	90	electron	electron	NOUN
fnp-5883	210	91	microscopy	microscopy	NOUN
fnp-5883	210	92	well	well	ADV
fnp-5883	210	93	preserved	preserve	VERB
fnp-5883	210	94	ultrastructure	ultrastructure	NOUN
fnp-5883	210	95	in	in	ADP
fnp-5883	210	96	surface	surface	NOUN
fnp-5883	210	97	areas	area	NOUN
fnp-5883	210	98	with	with	ADP
fnp-5883	210	99	ability	ability	NOUN
fnp-5883	210	100	to	to	PART
fnp-5883	210	101	detect	detect	VERB
fnp-5883	210	102	filaments	filament	NOUN
fnp-5883	210	103	;	;	PUNCT
fnp-5883	210	104	deeper	deep	ADJ
fnp-5883	210	105	regions	region	NOUN
fnp-5883	210	106	have	have	VERB
fnp-5883	210	107	ice	ice	NOUN
fnp-5883	210	108	damage	damage	NOUN
fnp-5883	210	109	1	1	NUM
fnp-5883	210	110	 	 	SPACE
fnp-5883	210	111	mm	mm	PROPN
fnp-5883	210	112	rat	rat	NOUN
fnp-5883	210	113	cerebral	cerebral	ADJ
fnp-5883	210	114	cortex	cortex	NOUN
fnp-5883	210	115	blocks	block	NOUN
fnp-5883	210	116	(	(	PUNCT
fnp-5883	210	117	linner	linner	NOUN
fnp-5883	210	118	et	et	PROPN
fnp-5883	210	119	al	al	PROPN
fnp-5883	210	120	.	.	PROPN
fnp-5883	210	121	,	,	PUNCT
fnp-5883	210	122	1986	1986	NUM
fnp-5883	210	123	)	)	PUNCT
fnp-5883	210	124	frozen	freeze	VERB
fnp-5883	210	125	against	against	ADP
fnp-5883	210	126	copper	copper	NOUN
fnp-5883	210	127	block	block	NOUN
fnp-5883	210	128	cooled	cool	VERB
fnp-5883	210	129	with	with	ADP
fnp-5883	210	130	liquid	liquid	ADJ
fnp-5883	210	131	nitrogen	nitrogen	NOUN
fnp-5883	210	132	vacuum	vacuum	NOUN
fnp-5883	210	133	drying	drying	NOUN
fnp-5883	210	134	,	,	PUNCT
fnp-5883	210	135	osmication	osmication	NOUN
fnp-5883	210	136	,	,	PUNCT
fnp-5883	210	137	electron	electron	NOUN
fnp-5883	210	138	microscopy	microscopy	NOUN
fnp-5883	210	139	excellent	excellent	ADJ
fnp-5883	210	140	preservation	preservation	NOUN
fnp-5883	210	141	with	with	ADP
fnp-5883	210	142	no	no	DET
fnp-5883	210	143	ice	ice	NOUN
fnp-5883	210	144	damage	damage	NOUN
fnp-5883	210	145	in	in	ADP
fnp-5883	210	146	first	first	ADJ
fnp-5883	210	147	10	10	NUM
fnp-5883	210	148	 	 	SPACE
fnp-5883	210	149	µm	µm	ADP
fnp-5883	210	150	;	;	PUNCT
fnp-5883	210	151	deeper	deep	ADJ
fnp-5883	210	152	,	,	PUNCT
fnp-5883	210	153	ice	ice	NOUN
fnp-5883	210	154	crystals	crystal	NOUN
fnp-5883	210	155	cause	cause	VERB
fnp-5883	210	156	damage	damage	NOUN
fnp-5883	210	157	by	by	ADP
fnp-5883	210	158	physically	physically	ADV
fnp-5883	210	159	compressing	compress	VERB
fnp-5883	210	160	tissue	tissue	NOUN
fnp-5883	210	161	rat	rat	NOUN
fnp-5883	210	162	neurointermediate	neurointermediate	NOUN
fnp-5883	210	163	lobe	lobe	PROPN
fnp-5883	210	164	(	(	PUNCT
fnp-5883	210	165	tian	tian	PROPN
fnp-5883	210	166	et	et	PROPN
fnp-5883	210	167	al	al	PROPN
fnp-5883	210	168	.	.	PROPN
fnp-5883	210	169	,	,	PUNCT
fnp-5883	210	170	1991	1991	NUM
fnp-5883	210	171	)	)	PUNCT
fnp-5883	210	172	falling	fall	VERB
fnp-5883	210	173	onto	onto	ADP
fnp-5883	210	174	copper	copper	NOUN
fnp-5883	210	175	plug	plug	NOUN
fnp-5883	210	176	cooled	cool	VERB
fnp-5883	210	177	with	with	ADP
fnp-5883	210	178	liquid	liquid	ADJ
fnp-5883	210	179	nitrogen	nitrogen	NOUN
fnp-5883	210	180	freeze	freeze	NOUN
fnp-5883	210	181	substitution	substitution	NOUN
fnp-5883	210	182	,	,	PUNCT
fnp-5883	210	183	electron	electron	NOUN
fnp-5883	210	184	microscopy	microscopy	NOUN
fnp-5883	210	185	good	good	ADJ
fnp-5883	210	186	morphology	morphology	NOUN
fnp-5883	210	187	in	in	ADP
fnp-5883	210	188	the	the	DET
fnp-5883	210	189	first	first	ADJ
fnp-5883	210	190	10–12	10–12	NUM
fnp-5883	210	191	 	 	SPACE
fnp-5883	210	192	µm	µm	ADP
fnp-5883	210	193	;	;	PUNCT
fnp-5883	210	194	deeper	deep	ADJ
fnp-5883	210	195	,	,	PUNCT
fnp-5883	210	196	ice	ice	NOUN
fnp-5883	210	197	crystals	crystal	NOUN
fnp-5883	210	198	make	make	VERB
fnp-5883	210	199	membranes	membrane	NOUN
fnp-5883	210	200	indistinct	indistinct	ADJ
fnp-5883	210	201	,	,	PUNCT
fnp-5883	210	202	and	and	CCONJ
fnp-5883	210	203	less	less	ADJ
fnp-5883	210	204	material	material	NOUN
fnp-5883	210	205	in	in	ADP
fnp-5883	210	206	extracellular	extracellular	ADJ
fnp-5883	210	207	space	space	NOUN
fnp-5883	210	208	200	200	NUM
fnp-5883	210	209	 	 	SPACE
fnp-5883	210	210	µm	µm	ADP
fnp-5883	210	211	thick	thick	ADJ
fnp-5883	210	212	sections	section	NOUN
fnp-5883	210	213	of	of	ADP
fnp-5883	210	214	mouse	mouse	NOUN
fnp-5883	210	215	cortex	cortex	NOUN
fnp-5883	210	216	(	(	PUNCT
fnp-5883	210	217	korogod	korogod	NOUN
fnp-5883	210	218	et	et	PROPN
fnp-5883	210	219	al	al	PROPN
fnp-5883	210	220	.	.	PROPN
fnp-5883	210	221	,	,	PUNCT
fnp-5883	210	222	2015	2015	NUM
fnp-5883	210	223	)	)	PUNCT
fnp-5883	210	224	high	high	ADJ
fnp-5883	210	225	-	-	PUNCT
fnp-5883	210	226	pressure	pressure	NOUN
fnp-5883	210	227	freezing	freezing	NOUN
fnp-5883	210	228	,	,	PUNCT
fnp-5883	210	229	storage	storage	NOUN
fnp-5883	210	230	at	at	ADP
fnp-5883	210	231	liquid	liquid	ADJ
fnp-5883	210	232	nitrogen	nitrogen	NOUN
fnp-5883	210	233	temperature	temperature	NOUN
fnp-5883	210	234	freeze	freeze	NOUN
fnp-5883	210	235	substitution	substitution	NOUN
fnp-5883	210	236	,	,	PUNCT
fnp-5883	210	237	electron	electron	NOUN
fnp-5883	210	238	microscopy	microscopy	NOUN
fnp-5883	210	239	at	at	ADP
fnp-5883	210	240	10	10	NUM
fnp-5883	210	241	 	 	SPACE
fnp-5883	210	242	µm	µm	NOUN
fnp-5883	210	243	into	into	ADP
fnp-5883	210	244	the	the	DET
fnp-5883	210	245	sample	sample	NOUN
fnp-5883	210	246	,	,	PUNCT
fnp-5883	210	247	high	high	ADJ
fnp-5883	210	248	quality	quality	NOUN
fnp-5883	210	249	ultrastructure	ultrastructure	NOUN
fnp-5883	210	250	;	;	PUNCT
fnp-5883	210	251	at	at	ADP
fnp-5883	210	252	50	50	NUM
fnp-5883	210	253	 	 	SPACE
fnp-5883	210	254	µm	µm	NOUN
fnp-5883	210	255	,	,	PUNCT
fnp-5883	210	256	cellular	cellular	ADJ
fnp-5883	210	257	elements	element	NOUN
fnp-5883	210	258	disrupted	disrupt	VERB
fnp-5883	210	259	in	in	ADP
fnp-5883	210	260	a	a	DET
fnp-5883	210	261	lattice	lattice	NOUN
fnp-5883	210	262	pattern	pattern	NOUN
fnp-5883	210	263	due	due	ADP
fnp-5883	210	264	to	to	ADP
fnp-5883	210	265	ice	ice	NOUN
fnp-5883	210	266	crystals	crystal	NOUN
fnp-5883	210	267	200	200	NUM
fnp-5883	210	268	 	 	SPACE
fnp-5883	210	269	µm	µm	ADP
fnp-5883	210	270	thick	thick	ADJ
fnp-5883	210	271	sections	section	NOUN
fnp-5883	210	272	of	of	ADP
fnp-5883	210	273	mouse	mouse	NOUN
fnp-5883	210	274	cortex	cortex	NOUN
fnp-5883	210	275	(	(	PUNCT
fnp-5883	210	276	dumoux	dumoux	VERB
fnp-5883	210	277	et	et	PROPN
fnp-5883	210	278	al	al	PROPN
fnp-5883	210	279	.	.	PROPN
fnp-5883	210	280	,	,	PUNCT
fnp-5883	210	281	2023	2023	NUM
fnp-5883	210	282	)	)	PUNCT
fnp-5883	210	283	cryoprotected	cryoprotecte	VERB
fnp-5883	210	284	in	in	ADP
fnp-5883	210	285	20	20	NUM
fnp-5883	210	286	 	 	SPACE
fnp-5883	210	287	%	%	NOUN
fnp-5883	210	288	bovine	bovine	NOUN
fnp-5883	210	289	serum	serum	NOUN
fnp-5883	210	290	albumin	albumin	NOUN
fnp-5883	210	291	,	,	PUNCT
fnp-5883	210	292	then	then	ADV
fnp-5883	210	293	high	high	ADJ
fnp-5883	210	294	-	-	PUNCT
fnp-5883	210	295	pressure	pressure	NOUN
fnp-5883	210	296	freezing	freeze	VERB
fnp-5883	210	297	cryo	cryo	NOUN
fnp-5883	210	298	-	-	PUNCT
fnp-5883	210	299	plasma	plasma	NOUN
fnp-5883	210	300	focused	focus	VERB
fnp-5883	210	301	ion	ion	NOUN
fnp-5883	210	302	beam	beam	NOUN
fnp-5883	210	303	scanning	scan	VERB
fnp-5883	210	304	electron	electron	NOUN
fnp-5883	210	305	microscopy	microscopy	NOUN
fnp-5883	210	306	high	high	ADJ
fnp-5883	210	307	-	-	PUNCT
fnp-5883	210	308	quality	quality	NOUN
fnp-5883	210	309	preservation	preservation	NOUN
fnp-5883	210	310	via	via	ADP
fnp-5883	210	311	vitrification	vitrification	NOUN
fnp-5883	210	312	,	,	PUNCT
fnp-5883	210	313	with	with	ADP
fnp-5883	210	314	structural	structural	ADJ
fnp-5883	210	315	preservation	preservation	NOUN
fnp-5883	210	316	of	of	ADP
fnp-5883	210	317	synapses	synapsis	NOUN
fnp-5883	210	318	,	,	PUNCT
fnp-5883	210	319	myelin	myelin	NOUN
fnp-5883	210	320	,	,	PUNCT
fnp-5883	210	321	and	and	CCONJ
fnp-5883	210	322	mitochondria	mitochondria	PROPN
fnp-5883	210	323	table	table	NOUN
fnp-5883	210	324	 	 	SPACE
fnp-5883	210	325	2	2	NUM
fnp-5883	210	326	.	.	NUM
fnp-5883	210	327	reported	report	VERB
fnp-5883	210	328	histologic	histologic	ADJ
fnp-5883	210	329	effects	effect	NOUN
fnp-5883	210	330	in	in	ADP
fnp-5883	210	331	representative	representative	ADJ
fnp-5883	210	332	observations	observation	NOUN
fnp-5883	210	333	from	from	ADP
fnp-5883	210	334	cryofixation	cryofixation	NOUN
fnp-5883	210	335	studies	study	NOUN
fnp-5883	210	336	.	.	PUNCT
fnp-5883	211	1	there	there	PRON
fnp-5883	211	2	is	be	VERB
fnp-5883	211	3	a	a	DET
fnp-5883	211	4	large	large	ADJ
fnp-5883	211	5	literature	literature	NOUN
fnp-5883	211	6	comparing	compare	VERB
fnp-5883	211	7	vitrification	vitrification	NOUN
fnp-5883	211	8	via	via	ADP
fnp-5883	211	9	cryofixation	cryofixation	NOUN
fnp-5883	211	10	to	to	ADP
fnp-5883	211	11	chemical	chemical	NOUN
fnp-5883	211	12	fixation	fixation	NOUN
fnp-5883	211	13	,	,	PUNCT
fnp-5883	211	14	which	which	PRON
fnp-5883	211	15	are	be	AUX
fnp-5883	211	16	two	two	NUM
fnp-5883	211	17	of	of	ADP
fnp-5883	211	18	the	the	DET
fnp-5883	211	19	major	major	ADJ
fnp-5883	211	20	ways	way	NOUN
fnp-5883	211	21	currently	currently	ADV
fnp-5883	211	22	available	available	ADJ
fnp-5883	211	23	to	to	PART
fnp-5883	211	24	ascertain	ascertain	VERB
fnp-5883	211	25	native	native	ADJ
fnp-5883	211	26	tissue	tissue	NOUN
fnp-5883	211	27	structure	structure	NOUN
fnp-5883	211	28	.	.	PUNCT
fnp-5883	212	1	in	in	ADP
fnp-5883	212	2	general	general	ADJ
fnp-5883	212	3	,	,	PUNCT
fnp-5883	212	4	most	most	ADJ
fnp-5883	212	5	studies	study	NOUN
fnp-5883	212	6	comparing	compare	VERB
fnp-5883	212	7	the	the	DET
fnp-5883	212	8	two	two	NUM
fnp-5883	212	9	methods	method	NOUN
fnp-5883	212	10	have	have	AUX
fnp-5883	212	11	found	find	VERB
fnp-5883	212	12	that	that	SCONJ
fnp-5883	212	13	the	the	DET
fnp-5883	212	14	ultrastructural	ultrastructural	ADJ
fnp-5883	212	15	appearance	appearance	NOUN
fnp-5883	212	16	is	be	AUX
fnp-5883	212	17	largely	largely	ADV
fnp-5883	212	18	similar	similar	ADJ
fnp-5883	212	19	,	,	PUNCT
fnp-5883	212	20	with	with	ADP
fnp-5883	212	21	cryofixation	cryofixation	NOUN
fnp-5883	212	22	sometimes	sometimes	ADV
fnp-5883	212	23	showing	show	VERB
fnp-5883	212	24	subtle	subtle	ADJ
fnp-5883	212	25	improvements	improvement	NOUN
fnp-5883	212	26	in	in	ADP
fnp-5883	212	27	the	the	DET
fnp-5883	212	28	preservation	preservation	NOUN
fnp-5883	212	29	of	of	ADP
fnp-5883	212	30	certain	certain	ADJ
fnp-5883	212	31	features	feature	NOUN
fnp-5883	212	32	(	(	PUNCT
fnp-5883	212	33	landis	landis	PROPN
fnp-5883	212	34	and	and	CCONJ
fnp-5883	212	35	reese	reese	PROPN
fnp-5883	212	36	,	,	PUNCT
fnp-5883	212	37	1981	1981	NUM
fnp-5883	212	38	,	,	PUNCT
fnp-5883	212	39	1983	1983	NUM
fnp-5883	212	40	;	;	PUNCT
fnp-5883	212	41	reger	reger	PROPN
fnp-5883	212	42	and	and	CCONJ
fnp-5883	212	43	escaig	escaig	VERB
fnp-5883	212	44	,	,	PUNCT
fnp-5883	212	45	1988	1988	NUM
fnp-5883	212	46	;	;	PUNCT
fnp-5883	212	47	zhao	zhao	PROPN
fnp-5883	212	48	et	et	PROPN
fnp-5883	212	49	al	al	PROPN
fnp-5883	212	50	.	.	PROPN
fnp-5883	212	51	,	,	PUNCT
fnp-5883	212	52	2012	2012	NUM
fnp-5883	212	53	;	;	PUNCT
fnp-5883	212	54	korogod	korogod	PROPN
fnp-5883	212	55	et	et	PROPN
fnp-5883	212	56	al	al	PROPN
fnp-5883	212	57	.	.	PROPN
fnp-5883	212	58	,	,	PUNCT
fnp-5883	212	59	2015	2015	NUM
fnp-5883	212	60	)	)	PUNCT
fnp-5883	212	61	.	.	PUNCT
fnp-5883	213	1	for	for	ADP
fnp-5883	213	2	example	example	NOUN
fnp-5883	213	3	,	,	PUNCT
fnp-5883	213	4	one	one	NUM
fnp-5883	213	5	study	study	NOUN
fnp-5883	213	6	found	find	VERB
fnp-5883	213	7	that	that	SCONJ
fnp-5883	213	8	cryofixation	cryofixation	NOUN
fnp-5883	213	9	of	of	ADP
fnp-5883	213	10	samples	sample	NOUN
fnp-5883	213	11	by	by	ADP
fnp-5883	213	12	placing	place	VERB
fnp-5883	213	13	them	they	PRON
fnp-5883	213	14	against	against	ADP
fnp-5883	213	15	a	a	DET
fnp-5883	213	16	metal	metal	NOUN
fnp-5883	213	17	surface	surface	NOUN
fnp-5883	213	18	cooled	cool	VERB
fnp-5883	213	19	to	to	ADP
fnp-5883	213	20	‑196	‑196	PROPN
fnp-5883	213	21	 	 	SPACE
fnp-5883	213	22	°	°	SCONJ
fnp-5883	213	23	c	c	NOUN
fnp-5883	213	24	better	well	ADV
fnp-5883	213	25	maintained	maintain	VERB
fnp-5883	213	26	the	the	DET
fnp-5883	213	27	naturally	naturally	ADV
fnp-5883	213	28	smooth	smooth	ADJ
fnp-5883	213	29	contour	contour	NOUN
fnp-5883	213	30	of	of	ADP
fnp-5883	213	31	cell	cell	NOUN
fnp-5883	213	32	processes	process	NOUN
fnp-5883	213	33	and	and	CCONJ
fnp-5883	213	34	organelles	organelle	NOUN
fnp-5883	213	35	(	(	PUNCT
fnp-5883	213	36	hirokawa	hirokawa	PROPN
fnp-5883	213	37	and	and	CCONJ
fnp-5883	213	38	kirino	kirino	PROPN
fnp-5883	213	39	,	,	PUNCT
fnp-5883	213	40	1980	1980	NUM
fnp-5883	213	41	)	)	PUNCT
fnp-5883	213	42	.	.	PUNCT
fnp-5883	214	1	the	the	DET
fnp-5883	214	2	authors	author	NOUN
fnp-5883	214	3	also	also	ADV
fnp-5883	214	4	suggested	suggest	VERB
fnp-5883	214	5	that	that	SCONJ
fnp-5883	214	6	cryofixation	cryofixation	NOUN
fnp-5883	214	7	appears	appear	VERB
fnp-5883	214	8	to	to	PART
fnp-5883	214	9	better	well	ADV
fnp-5883	214	10	preserve	preserve	VERB
fnp-5883	214	11	the	the	DET
fnp-5883	214	12	extracellular	extracellular	ADJ
fnp-5883	214	13	spaces	space	NOUN
fnp-5883	214	14	and	and	CCONJ
fnp-5883	214	15	better	well	ADV
fnp-5883	214	16	retain	retain	VERB
fnp-5883	214	17	water	water	NOUN
fnp-5883	214	18	-	-	PUNCT
fnp-5883	214	19	soluble	soluble	ADJ
fnp-5883	214	20	components	component	NOUN
fnp-5883	214	21	.	.	PUNCT
fnp-5883	215	1	better	well	ADJ
fnp-5883	215	2	maintenance	maintenance	NOUN
fnp-5883	215	3	of	of	ADP
fnp-5883	215	4	water	water	NOUN
fnp-5883	215	5	-	-	PUNCT
fnp-5883	215	6	soluble	soluble	ADJ
fnp-5883	215	7	components	component	NOUN
fnp-5883	215	8	,	,	PUNCT
fnp-5883	215	9	may	may	AUX
fnp-5883	215	10	in	in	ADP
fnp-5883	215	11	turn	turn	NOUN
fnp-5883	215	12	lead	lead	VERB
fnp-5883	215	13	to	to	ADP
fnp-5883	215	14	a	a	DET
fnp-5883	215	15	more	more	ADV
fnp-5883	215	16	natural	natural	ADJ
fnp-5883	215	17	density	density	NOUN
fnp-5883	215	18	of	of	ADP
fnp-5883	215	19	the	the	DET
fnp-5883	215	20	cytoplasm	cytoplasm	NOUN
fnp-5883	215	21	and	and	CCONJ
fnp-5883	215	22	the	the	DET
fnp-5883	215	23	mitochondrial	mitochondrial	ADJ
fnp-5883	215	24	matrix	matrix	NOUN
fnp-5883	215	25	(	(	PUNCT
fnp-5883	215	26	hirokawa	hirokawa	PROPN
fnp-5883	215	27	and	and	CCONJ
fnp-5883	215	28	kirino	kirino	PROPN
fnp-5883	215	29	,	,	PUNCT
fnp-5883	215	30	1980	1980	NUM
fnp-5883	215	31	)	)	PUNCT
fnp-5883	215	32	.	.	PUNCT
fnp-5883	216	1	it	it	PRON
fnp-5883	216	2	has	have	AUX
fnp-5883	216	3	been	be	AUX
fnp-5883	216	4	reported	report	VERB
fnp-5883	216	5	that	that	SCONJ
fnp-5883	216	6	microtubules	microtubule	NOUN
fnp-5883	216	7	are	be	AUX
fnp-5883	216	8	better	well	ADV
fnp-5883	216	9	preserved	preserve	VERB
fnp-5883	216	10	after	after	ADP
fnp-5883	216	11	cryofixation	cryofixation	NOUN
fnp-5883	216	12	(	(	PUNCT
fnp-5883	216	13	moor	moor	PROPN
fnp-5883	216	14	et	et	PROPN
fnp-5883	216	15	al	al	PROPN
fnp-5883	216	16	.	.	PROPN
fnp-5883	216	17	,	,	PUNCT
fnp-5883	216	18	1980	1980	NUM
fnp-5883	216	19	)	)	PUNCT
fnp-5883	216	20	.	.	PUNCT
fnp-5883	217	1	cryofixation	cryofixation	NOUN
fnp-5883	217	2	also	also	ADV
fnp-5883	217	3	leads	lead	VERB
fnp-5883	217	4	to	to	ADP
fnp-5883	217	5	better	well	ADJ
fnp-5883	217	6	preservation	preservation	NOUN
fnp-5883	217	7	of	of	ADP
fnp-5883	217	8	antigenicity	antigenicity	NOUN
fnp-5883	217	9	(	(	PUNCT
fnp-5883	217	10	hisano	hisano	PROPN
fnp-5883	217	11	et	et	PROPN
fnp-5883	217	12	al	al	PROPN
fnp-5883	217	13	.	.	PROPN
fnp-5883	217	14	,	,	PUNCT
fnp-5883	217	15	1986	1986	NUM
fnp-5883	217	16	)	)	PUNCT
fnp-5883	217	17	.	.	PUNCT
fnp-5883	218	1	finally	finally	ADV
fnp-5883	218	2	,	,	PUNCT
fnp-5883	218	3	cryofixation	cryofixation	NOUN
fnp-5883	218	4	likely	likely	ADV
fnp-5883	218	5	preserves	preserve	VERB
fnp-5883	218	6	a	a	DET
fnp-5883	218	7	more	more	ADV
fnp-5883	218	8	natural	natural	ADJ
fnp-5883	218	9	distribution	distribution	NOUN
fnp-5883	218	10	of	of	ADP
fnp-5883	218	11	water	water	NOUN
fnp-5883	218	12	and	and	CCONJ
fnp-5883	218	13	ions	ion	NOUN
fnp-5883	218	14	between	between	ADP
fnp-5883	218	15	intracellular	intracellular	ADJ
fnp-5883	218	16	and	and	CCONJ
fnp-5883	218	17	extracellular	extracellular	ADJ
fnp-5883	218	18	compartments	compartment	NOUN
fnp-5883	218	19	(	(	PUNCT
fnp-5883	218	20	harreveld	harreveld	NOUN
fnp-5883	218	21	and	and	CCONJ
fnp-5883	218	22	fifkova	fifkova	PROPN
fnp-5883	218	23	,	,	PUNCT
fnp-5883	218	24	1975	1975	NUM
fnp-5883	218	25	;	;	PUNCT
fnp-5883	218	26	moor	moor	PROPN
fnp-5883	218	27	et	et	PROPN
fnp-5883	218	28	al	al	PROPN
fnp-5883	218	29	.	.	PROPN
fnp-5883	218	30	,	,	PUNCT
fnp-5883	218	31	1980	1980	NUM
fnp-5883	218	32	)	)	PUNCT
fnp-5883	218	33	.	.	PUNCT
fnp-5883	219	1	however	however	ADV
fnp-5883	219	2	,	,	PUNCT
fnp-5883	219	3	despite	despite	SCONJ
fnp-5883	219	4	these	these	DET
fnp-5883	219	5	benefits	benefit	NOUN
fnp-5883	219	6	,	,	PUNCT
fnp-5883	219	7	the	the	DET
fnp-5883	219	8	differences	difference	NOUN
fnp-5883	219	9	between	between	ADP
fnp-5883	219	10	cryofixation	cryofixation	NOUN
fnp-5883	219	11	and	and	CCONJ
fnp-5883	219	12	chemical	chemical	NOUN
fnp-5883	219	13	fixation	fixation	NOUN
fnp-5883	219	14	are	be	AUX
fnp-5883	219	15	generally	generally	ADV
fnp-5883	219	16	subtle	subtle	ADJ
fnp-5883	219	17	when	when	SCONJ
fnp-5883	219	18	both	both	DET
fnp-5883	219	19	techniques	technique	NOUN
fnp-5883	219	20	are	be	AUX
fnp-5883	219	21	optimally	optimally	ADV
fnp-5883	219	22	performed	perform	VERB
fnp-5883	219	23	.	.	PUNCT
fnp-5883	220	1	indeed	indeed	ADV
fnp-5883	220	2	,	,	PUNCT
fnp-5883	220	3	tissue	tissue	NOUN
fnp-5883	220	4	that	that	PRON
fnp-5883	220	5	is	be	AUX
fnp-5883	220	6	chemically	chemically	ADV
fnp-5883	220	7	fixed	fix	VERB
fnp-5883	220	8	and	and	CCONJ
fnp-5883	220	9	cryoprotected	cryoprotecte	VERB
fnp-5883	220	10	with	with	ADP
fnp-5883	220	11	glycerol	glycerol	NOUN
fnp-5883	220	12	has	have	AUX
fnp-5883	220	13	been	be	AUX
fnp-5883	220	14	used	use	VERB
fnp-5883	220	15	as	as	ADP
fnp-5883	220	16	a	a	DET
fnp-5883	220	17	control	control	NOUN
fnp-5883	220	18	to	to	PART
fnp-5883	220	19	ensure	ensure	VERB
fnp-5883	220	20	that	that	SCONJ
fnp-5883	220	21	the	the	DET
fnp-5883	220	22	cryofixation	cryofixation	NOUN
fnp-5883	220	23	procedure	procedure	NOUN
fnp-5883	220	24	adequately	adequately	ADV
fnp-5883	220	25	preserves	preserve	VERB
fnp-5883	220	26	the	the	DET
fnp-5883	220	27	structure	structure	NOUN
fnp-5883	220	28	of	of	ADP
fnp-5883	220	29	the	the	DET
fnp-5883	220	30	tissue	tissue	NOUN
fnp-5883	220	31	(	(	PUNCT
fnp-5883	220	32	heuser	heuser	NOUN
fnp-5883	220	33	et	et	PROPN
fnp-5883	220	34	al	al	PROPN
fnp-5883	220	35	.	.	PROPN
fnp-5883	220	36	,	,	PUNCT
fnp-5883	220	37	1976	1976	NUM
fnp-5883	220	38	)	)	PUNCT
fnp-5883	220	39	.	.	PUNCT
fnp-5883	221	1	many	many	ADJ
fnp-5883	221	2	of	of	ADP
fnp-5883	221	3	the	the	DET
fnp-5883	221	4	chemical	chemical	NOUN
fnp-5883	221	5	fixation	fixation	NOUN
fnp-5883	221	6	artifacts	artifact	NOUN
fnp-5883	221	7	that	that	PRON
fnp-5883	221	8	have	have	AUX
fnp-5883	221	9	been	be	AUX
fnp-5883	221	10	described	describe	VERB
fnp-5883	221	11	,	,	PUNCT
fnp-5883	221	12	such	such	ADJ
fnp-5883	221	13	as	as	ADP
fnp-5883	221	14	cell	cell	NOUN
fnp-5883	221	15	shrinkage	shrinkage	NOUN
fnp-5883	221	16	and	and	CCONJ
fnp-5883	221	17	membrane	membrane	NOUN
fnp-5883	221	18	discontinuities	discontinuity	NOUN
fnp-5883	221	19	,	,	PUNCT
fnp-5883	221	20	appear	appear	VERB
fnp-5883	221	21	to	to	PART
fnp-5883	221	22	be	be	AUX
fnp-5883	221	23	largely	largely	ADV
fnp-5883	221	24	related	relate	VERB
fnp-5883	221	25	to	to	ADP
fnp-5883	221	26	the	the	DET
fnp-5883	221	27	tissue	tissue	NOUN
fnp-5883	221	28	processing	processing	NOUN
fnp-5883	221	29	steps	step	NOUN
fnp-5883	221	30	after	after	ADP
fnp-5883	221	31	fixation	fixation	NOUN
fnp-5883	221	32	rather	rather	ADV
fnp-5883	221	33	than	than	ADP
fnp-5883	221	34	due	due	ADP
fnp-5883	221	35	to	to	ADP
fnp-5883	221	36	the	the	DET
fnp-5883	221	37	initial	initial	ADJ
fnp-5883	221	38	fixation	fixation	NOUN
fnp-5883	221	39	itself	itself	PRON
fnp-5883	221	40	(	(	PUNCT
fnp-5883	221	41	sosinsky	sosinsky	PROPN
fnp-5883	221	42	et	et	PROPN
fnp-5883	221	43	al	al	PROPN
fnp-5883	221	44	.	.	PROPN
fnp-5883	221	45	,	,	PUNCT
fnp-5883	221	46	2008	2008	NUM
fnp-5883	221	47	)	)	PUNCT
fnp-5883	221	48	.	.	PUNCT
fnp-5883	222	1	ice	ice	NOUN
fnp-5883	222	2	damage	damage	NOUN
fnp-5883	222	3	cryofixation	cryofixation	NOUN
fnp-5883	222	4	can	can	AUX
fnp-5883	222	5	enable	enable	VERB
fnp-5883	222	6	excellent	excellent	ADJ
fnp-5883	222	7	ultrastructural	ultrastructural	ADJ
fnp-5883	222	8	preservation	preservation	NOUN
fnp-5883	222	9	in	in	ADP
fnp-5883	222	10	successfully	successfully	ADV
fnp-5883	222	11	vitrified	vitrify	VERB
fnp-5883	222	12	regions	region	NOUN
fnp-5883	222	13	.	.	PUNCT
fnp-5883	223	1	however	however	ADV
fnp-5883	223	2	,	,	PUNCT
fnp-5883	223	3	it	it	PRON
fnp-5883	223	4	is	be	AUX
fnp-5883	223	5	expected	expect	VERB
fnp-5883	223	6	that	that	SCONJ
fnp-5883	223	7	in	in	ADP
fnp-5883	223	8	sufficiently	sufficiently	ADV
fnp-5883	223	9	deep	deep	ADJ
fnp-5883	223	10	areas	area	NOUN
fnp-5883	223	11	of	of	ADP
fnp-5883	223	12	the	the	DET
fnp-5883	223	13	tissue	tissue	NOUN
fnp-5883	223	14	,	,	PUNCT
fnp-5883	223	15	where	where	SCONJ
fnp-5883	223	16	the	the	DET
fnp-5883	223	17	cooling	cool	VERB
fnp-5883	223	18	rate	rate	NOUN
fnp-5883	223	19	is	be	AUX
fnp-5883	223	20	insufficient	insufficient	ADJ
fnp-5883	223	21	to	to	PART
fnp-5883	223	22	achieve	achieve	VERB
fnp-5883	223	23	vitrification	vitrification	NOUN
fnp-5883	223	24	,	,	PUNCT
fnp-5883	223	25	there	there	PRON
fnp-5883	223	26	will	will	AUX
fnp-5883	223	27	be	be	AUX
fnp-5883	223	28	ice	ice	NOUN
fnp-5883	223	29	crystal	crystal	NOUN
fnp-5883	223	30	formation	formation	NOUN
fnp-5883	223	31	.	.	PUNCT
fnp-5883	224	1	the	the	DET
fnp-5883	224	2	effects	effect	NOUN
fnp-5883	224	3	of	of	ADP
fnp-5883	224	4	ice	ice	NOUN
fnp-5883	224	5	crystals	crystal	NOUN
fnp-5883	224	6	on	on	ADP
fnp-5883	224	7	tissue	tissue	NOUN
fnp-5883	224	8	morphology	morphology	NOUN
fnp-5883	224	9	manifest	manifest	NOUN
fnp-5883	224	10	depending	depend	VERB
fnp-5883	224	11	on	on	ADP
fnp-5883	224	12	the	the	DET
fnp-5883	224	13	depth	depth	NOUN
fnp-5883	224	14	of	of	ADP
fnp-5883	224	15	the	the	DET
fnp-5883	224	16	tissue	tissue	NOUN
fnp-5883	224	17	and	and	CCONJ
fnp-5883	224	18	the	the	DET
fnp-5883	224	19	size	size	NOUN
fnp-5883	224	20	of	of	ADP
fnp-5883	224	21	the	the	DET
fnp-5883	224	22	crystals	crystal	NOUN
fnp-5883	224	23	.	.	PUNCT
fnp-5883	225	1	in	in	ADP
fnp-5883	225	2	the	the	DET
fnp-5883	225	3	areas	area	NOUN
fnp-5883	225	4	that	that	PRON
fnp-5883	225	5	border	border	NOUN
fnp-5883	225	6	vitrified	vitrify	VERB
fnp-5883	225	7	tissue	tissue	NOUN
fnp-5883	225	8	,	,	PUNCT
fnp-5883	225	9	where	where	SCONJ
fnp-5883	225	10	the	the	DET
fnp-5883	225	11	cooling	cool	VERB
fnp-5883	225	12	rate	rate	NOUN
fnp-5883	225	13	becomes	become	VERB
fnp-5883	225	14	too	too	ADV
fnp-5883	225	15	slow	slow	ADJ
fnp-5883	225	16	to	to	PART
fnp-5883	225	17	vitrify	vitrify	VERB
fnp-5883	225	18	the	the	DET
fnp-5883	225	19	tissue	tissue	NOUN
fnp-5883	225	20	,	,	PUNCT
fnp-5883	225	21	small	small	ADJ
fnp-5883	225	22	ice	ice	NOUN
fnp-5883	225	23	crystals	crystal	NOUN
fnp-5883	225	24	and	and	CCONJ
fnp-5883	225	25	more	more	ADV
fnp-5883	225	26	subtle	subtle	ADJ
fnp-5883	225	27	signs	sign	NOUN
fnp-5883	225	28	of	of	ADP
fnp-5883	225	29	suboptimal	suboptimal	ADJ
fnp-5883	225	30	preservation	preservation	NOUN
fnp-5883	225	31	are	be	AUX
fnp-5883	225	32	expected	expect	VERB
fnp-5883	225	33	.	.	PUNCT
fnp-5883	226	1	for	for	ADP
fnp-5883	226	2	example	example	NOUN
fnp-5883	226	3	,	,	PUNCT
fnp-5883	226	4	there	there	PRON
fnp-5883	226	5	may	may	AUX
fnp-5883	226	6	be	be	AUX
fnp-5883	226	7	blebbing	blebbe	VERB
fnp-5883	226	8	of	of	ADP
fnp-5883	226	9	the	the	DET
fnp-5883	226	10	nuclear	nuclear	ADJ
fnp-5883	226	11	envelope	envelope	NOUN
fnp-5883	226	12	or	or	CCONJ
fnp-5883	226	13	alterations	alteration	NOUN
fnp-5883	226	14	of	of	ADP
fnp-5883	226	15	the	the	DET
fnp-5883	226	16	mitochondria	mitochondria	NOUN
fnp-5883	226	17	(	(	PUNCT
fnp-5883	226	18	möbius	möbius	PROPN
fnp-5883	226	19	et	et	PROPN
fnp-5883	226	20	al	al	PROPN
fnp-5883	226	21	.	.	PROPN
fnp-5883	226	22	,	,	PUNCT
fnp-5883	226	23	2010	2010	NUM
fnp-5883	226	24	)	)	PUNCT
fnp-5883	226	25	.	.	PUNCT
fnp-5883	227	1	membranes	membrane	NOUN
fnp-5883	227	2	may	may	AUX
fnp-5883	227	3	not	not	PART
fnp-5883	227	4	show	show	VERB
fnp-5883	227	5	their	their	PRON
fnp-5883	227	6	clear	clear	ADJ
fnp-5883	227	7	expected	expect	VERB
fnp-5883	227	8	structure	structure	NOUN
fnp-5883	227	9	(	(	PUNCT
fnp-5883	227	10	senda	senda	PROPN
fnp-5883	227	11	et	et	PROPN
fnp-5883	227	12	al	al	PROPN
fnp-5883	227	13	.	.	PROPN
fnp-5883	227	14	,	,	PUNCT
fnp-5883	227	15	2005	2005	NUM
fnp-5883	227	16	)	)	PUNCT
fnp-5883	227	17	.	.	PUNCT
fnp-5883	228	1	in	in	ADP
fnp-5883	228	2	deeper	deep	ADJ
fnp-5883	228	3	areas	area	NOUN
fnp-5883	228	4	,	,	PUNCT
fnp-5883	228	5	the	the	DET
fnp-5883	228	6	ice	ice	NOUN
fnp-5883	228	7	crystals	crystal	NOUN
fnp-5883	228	8	are	be	AUX
fnp-5883	228	9	expected	expect	VERB
fnp-5883	228	10	to	to	PART
fnp-5883	228	11	be	be	AUX
fnp-5883	228	12	larger	large	ADJ
fnp-5883	228	13	and	and	CCONJ
fnp-5883	228	14	to	to	PART
fnp-5883	228	15	increasingly	increasingly	ADV
fnp-5883	228	16	distort	distort	VERB
fnp-5883	228	17	cellular	cellular	ADJ
fnp-5883	228	18	structures	structure	NOUN
fnp-5883	228	19	,	,	PUNCT
fnp-5883	228	20	pressing	press	VERB
fnp-5883	228	21	the	the	DET
fnp-5883	228	22	latter	latter	ADJ
fnp-5883	228	23	into	into	ADP
fnp-5883	228	24	one	one	NUM
fnp-5883	228	25	another	another	DET
fnp-5883	228	26	,	,	PUNCT
fnp-5883	228	27	and	and	CCONJ
fnp-5883	228	28	causing	cause	VERB
fnp-5883	228	29	large	large	ADJ
fnp-5883	228	30	areas	area	NOUN
fnp-5883	228	31	to	to	PART
fnp-5883	228	32	be	be	AUX
fnp-5883	228	33	entirely	entirely	ADV
fnp-5883	228	34	devoid	devoid	ADJ
fnp-5883	228	35	of	of	ADP
fnp-5883	228	36	structure	structure	NOUN
fnp-5883	228	37	(	(	PUNCT
fnp-5883	228	38	heuser	heuser	NOUN
fnp-5883	228	39	et	et	PROPN
fnp-5883	228	40	al	al	PROPN
fnp-5883	228	41	.	.	PROPN
fnp-5883	228	42	,	,	PUNCT
fnp-5883	228	43	1976	1976	NUM
fnp-5883	228	44	;	;	PUNCT
fnp-5883	228	45	senda	senda	PROPN
fnp-5883	228	46	et	et	PROPN
fnp-5883	228	47	al	al	PROPN
fnp-5883	228	48	.	.	PROPN
fnp-5883	228	49	,	,	PUNCT
fnp-5883	228	50	2005	2005	NUM
fnp-5883	228	51	)	)	PUNCT
fnp-5883	228	52	.	.	PUNCT
fnp-5883	229	1	several	several	ADJ
fnp-5883	229	2	studies	study	NOUN
fnp-5883	229	3	also	also	ADV
fnp-5883	229	4	describe	describe	VERB
fnp-5883	229	5	ice	ice	NOUN
fnp-5883	229	6	artifacts	artifact	NOUN
fnp-5883	229	7	in	in	ADP
fnp-5883	229	8	deeper	deep	ADJ
fnp-5883	229	9	regions	region	NOUN
fnp-5883	229	10	as	as	ADP
fnp-5883	229	11	causing	cause	VERB
fnp-5883	229	12	reticulation	reticulation	NOUN
fnp-5883	229	13	of	of	ADP
fnp-5883	229	14	intracellular	intracellular	ADJ
fnp-5883	229	15	areas	area	NOUN
fnp-5883	229	16	(	(	PUNCT
fnp-5883	229	17	linner	linner	NOUN
fnp-5883	229	18	et	et	PROPN
fnp-5883	229	19	al	al	PROPN
fnp-5883	229	20	.	.	PROPN
fnp-5883	229	21	,	,	PUNCT
fnp-5883	229	22	1986	1986	NUM
fnp-5883	229	23	;	;	PUNCT
fnp-5883	229	24	siksou	siksou	NOUN
fnp-5883	229	25	et	et	PROPN
fnp-5883	229	26	al	al	PROPN
fnp-5883	229	27	.	.	PROPN
fnp-5883	229	28	,	,	PUNCT
fnp-5883	229	29	2007	2007	NUM
fnp-5883	229	30	;	;	PUNCT
fnp-5883	229	31	möbius	möbius	PROPN
fnp-5883	229	32	et	et	PROPN
fnp-5883	229	33	al	al	PROPN
fnp-5883	229	34	.	.	PROPN
fnp-5883	229	35	,	,	PUNCT
fnp-5883	229	36	2010	2010	NUM
fnp-5883	229	37	)	)	PUNCT
fnp-5883	229	38	.	.	PUNCT
fnp-5883	230	1	damage	damage	NOUN
fnp-5883	230	2	by	by	ADP
fnp-5883	230	3	tissue	tissue	NOUN
fnp-5883	230	4	cutting	cutting	NOUN
fnp-5883	230	5	and	and	CCONJ
fnp-5883	230	6	handling	handling	NOUN
fnp-5883	230	7	to	to	PART
fnp-5883	230	8	isolate	isolate	VERB
fnp-5883	230	9	a	a	DET
fnp-5883	230	10	sample	sample	NOUN
fnp-5883	230	11	that	that	PRON
fnp-5883	230	12	is	be	AUX
fnp-5883	230	13	sufficiently	sufficiently	ADV
fnp-5883	230	14	thin	thin	ADJ
fnp-5883	230	15	for	for	ADP
fnp-5883	230	16	cryofixation	cryofixation	NOUN
fnp-5883	230	17	,	,	PUNCT
fnp-5883	230	18	cutting	cut	VERB
fnp-5883	230	19	the	the	DET
fnp-5883	230	20	tissue	tissue	NOUN
fnp-5883	230	21	is	be	AUX
fnp-5883	230	22	generally	generally	ADV
fnp-5883	230	23	required	require	VERB
fnp-5883	230	24	.	.	PUNCT
fnp-5883	231	1	this	this	PRON
fnp-5883	231	2	in	in	ADP
fnp-5883	231	3	turn	turn	NOUN
fnp-5883	231	4	leads	lead	VERB
fnp-5883	231	5	to	to	PART
fnp-5883	231	6	compression	compression	VERB
fnp-5883	231	7	artifacts	artifact	NOUN
fnp-5883	231	8	near	near	ADP
fnp-5883	231	9	the	the	DET
fnp-5883	231	10	cut	cut	ADJ
fnp-5883	231	11	surface	surface	NOUN
fnp-5883	231	12	of	of	ADP
fnp-5883	231	13	the	the	DET
fnp-5883	231	14	tissue	tissue	NOUN
fnp-5883	231	15	,	,	PUNCT
fnp-5883	231	16	which	which	PRON
fnp-5883	231	17	can	can	AUX
fnp-5883	231	18	be	be	AUX
fnp-5883	231	19	severe	severe	ADJ
fnp-5883	231	20	(	(	PUNCT
fnp-5883	231	21	zuber	zuber	PROPN
fnp-5883	231	22	et	et	PROPN
fnp-5883	231	23	al	al	PROPN
fnp-5883	231	24	.	.	PROPN
fnp-5883	231	25	,	,	PUNCT
fnp-5883	231	26	2005	2005	NUM
fnp-5883	231	27	)	)	PUNCT
fnp-5883	231	28	.	.	PUNCT
fnp-5883	232	1	one	one	NUM
fnp-5883	232	2	study	study	NOUN
fnp-5883	232	3	omitted	omit	VERB
fnp-5883	232	4	the	the	DET
fnp-5883	232	5	first	first	ADJ
fnp-5883	232	6	20	20	NUM
fnp-5883	232	7	 	 	SPACE
fnp-5883	232	8	µm	µm	NOUN
fnp-5883	232	9	of	of	ADP
fnp-5883	232	10	the	the	DET
fnp-5883	232	11	sample	sample	NOUN
fnp-5883	232	12	from	from	ADP
fnp-5883	232	13	their	their	PRON
fnp-5883	232	14	analysis	analysis	NOUN
fnp-5883	232	15	as	as	ADP
fnp-5883	232	16	a	a	DET
fnp-5883	232	17	result	result	NOUN
fnp-5883	232	18	of	of	ADP
fnp-5883	232	19	these	these	DET
fnp-5883	232	20	artifacts	artifact	NOUN
fnp-5883	232	21	(	(	PUNCT
fnp-5883	232	22	borges	borge	NOUN
fnp-5883	232	23	-	-	PUNCT
fnp-5883	232	24	merjane	merjane	ADJ
fnp-5883	232	25	et	et	PROPN
fnp-5883	232	26	al	al	PROPN
fnp-5883	232	27	.	.	PROPN
fnp-5883	232	28	,	,	PUNCT
fnp-5883	232	29	2020	2020	NUM
fnp-5883	232	30	)	)	PUNCT
fnp-5883	232	31	.	.	PUNCT
fnp-5883	233	1	another	another	DET
fnp-5883	233	2	study	study	NOUN
fnp-5883	233	3	noted	note	VERB
fnp-5883	233	4	that	that	SCONJ
fnp-5883	233	5	dissection	dissection	NOUN
fnp-5883	233	6	led	lead	VERB
fnp-5883	233	7	to	to	ADP
fnp-5883	233	8	ultrastructural	ultrastructural	ADJ
fnp-5883	233	9	artifacts	artifact	NOUN
fnp-5883	233	10	within	within	ADP
fnp-5883	233	11	100	100	NUM
fnp-5883	233	12	 	 	SPACE
fnp-5883	233	13	µm	µm	NOUN
fnp-5883	233	14	from	from	ADP
fnp-5883	233	15	the	the	DET
fnp-5883	233	16	cutting	cut	VERB
fnp-5883	233	17	site	site	NOUN
fnp-5883	233	18	(	(	PUNCT
fnp-5883	233	19	siksou	siksou	INTJ
fnp-5883	233	20	et	et	PROPN
fnp-5883	233	21	al	al	PROPN
fnp-5883	233	22	.	.	PROPN
fnp-5883	233	23	,	,	PUNCT
fnp-5883	233	24	2007	2007	NUM
fnp-5883	233	25	)	)	PUNCT
fnp-5883	233	26	.	.	PUNCT
fnp-5883	234	1	handling	handle	VERB
fnp-5883	234	2	artifacts	artifact	NOUN
fnp-5883	234	3	that	that	PRON
fnp-5883	234	4	occur	occur	VERB
fnp-5883	234	5	during	during	ADP
fnp-5883	234	6	preparation	preparation	NOUN
fnp-5883	234	7	for	for	ADP
fnp-5883	234	8	cryofixation	cryofixation	NOUN
fnp-5883	234	9	have	have	AUX
fnp-5883	234	10	also	also	ADV
fnp-5883	234	11	been	be	AUX
fnp-5883	234	12	reported	report	VERB
fnp-5883	234	13	to	to	PART
fnp-5883	234	14	cause	cause	VERB
fnp-5883	234	15	detachment	detachment	NOUN
fnp-5883	234	16	of	of	ADP
fnp-5883	234	17	myelin	myelin	ADJ
fnp-5883	234	18	lamellae	lamellae	NOUN
fnp-5883	234	19	or	or	CCONJ
fnp-5883	234	20	separation	separation	NOUN
fnp-5883	234	21	of	of	ADP
fnp-5883	234	22	axons	axon	NOUN
fnp-5883	234	23	from	from	ADP
fnp-5883	234	24	the	the	DET
fnp-5883	234	25	myelin	myelin	NOUN
fnp-5883	234	26	sheath	sheath	NOUN
fnp-5883	234	27	(	(	PUNCT
fnp-5883	234	28	möbius	möbius	PROPN
fnp-5883	234	29	et	et	PROPN
fnp-5883	234	30	al	al	PROPN
fnp-5883	234	31	.	.	PROPN
fnp-5883	234	32	,	,	PUNCT
fnp-5883	234	33	2010	2010	NUM
fnp-5883	234	34	)	)	PUNCT
fnp-5883	234	35	.	.	PUNCT
fnp-5883	235	1	summary	summary	VERB
fnp-5883	235	2	the	the	DET
fnp-5883	235	3	major	major	ADJ
fnp-5883	235	4	limitation	limitation	NOUN
fnp-5883	235	5	of	of	ADP
fnp-5883	235	6	cryofixation	cryofixation	NOUN
fnp-5883	235	7	methods	method	NOUN
fnp-5883	235	8	is	be	AUX
fnp-5883	235	9	their	their	PRON
fnp-5883	235	10	inability	inability	NOUN
fnp-5883	235	11	to	to	PART
fnp-5883	235	12	preserve	preserve	VERB
fnp-5883	235	13	large	large	ADJ
fnp-5883	235	14	tissue	tissue	NOUN
fnp-5883	235	15	volumes	volume	NOUN
fnp-5883	235	16	without	without	ADP
fnp-5883	235	17	ice	ice	NOUN
fnp-5883	235	18	damage	damage	NOUN
fnp-5883	235	19	in	in	ADP
fnp-5883	235	20	the	the	DET
fnp-5883	235	21	sample	sample	NOUN
fnp-5883	235	22	core	core	NOUN
fnp-5883	235	23	.	.	PUNCT
fnp-5883	236	1	although	although	SCONJ
fnp-5883	236	2	regions	region	NOUN
fnp-5883	236	3	close	close	ADJ
fnp-5883	236	4	to	to	ADP
fnp-5883	236	5	the	the	DET
fnp-5883	236	6	surface	surface	NOUN
fnp-5883	236	7	of	of	ADP
fnp-5883	236	8	a	a	DET
fnp-5883	236	9	tissue	tissue	NOUN
fnp-5883	236	10	sample	sample	NOUN
fnp-5883	236	11	can	can	AUX
fnp-5883	236	12	be	be	AUX
fnp-5883	236	13	exquisitely	exquisitely	ADV
fnp-5883	236	14	preserved	preserve	VERB
fnp-5883	236	15	in	in	ADP
fnp-5883	236	16	a	a	DET
fnp-5883	236	17	vitrified	vitrified	ADJ
fnp-5883	236	18	state	state	NOUN
fnp-5883	236	19	,	,	PUNCT
fnp-5883	236	20	deeper	deep	ADJ
fnp-5883	236	21	regions	region	NOUN
fnp-5883	236	22	suffer	suffer	VERB
fnp-5883	236	23	from	from	ADP
fnp-5883	236	24	progressively	progressively	ADV
fnp-5883	236	25	more	more	ADV
fnp-5883	236	26	severe	severe	ADJ
fnp-5883	236	27	freezing	freezing	NOUN
fnp-5883	236	28	artifacts	artifact	NOUN
fnp-5883	236	29	due	due	ADJ
fnp-5883	236	30	to	to	ADP
fnp-5883	236	31	slower	slow	ADJ
fnp-5883	236	32	cooling	cool	VERB
fnp-5883	236	33	rates	rate	NOUN
fnp-5883	236	34	.	.	PUNCT
fnp-5883	237	1	increasing	increase	VERB
fnp-5883	237	2	the	the	DET
fnp-5883	237	3	pressure	pressure	NOUN
fnp-5883	237	4	during	during	ADP
fnp-5883	237	5	freezing	freezing	NOUN
fnp-5883	237	6	or	or	CCONJ
fnp-5883	237	7	using	use	VERB
fnp-5883	237	8	cryoprotectants	cryoprotectant	NOUN
fnp-5883	237	9	can	can	AUX
fnp-5883	237	10	reduce	reduce	VERB
fnp-5883	237	11	the	the	DET
fnp-5883	237	12	critical	critical	ADJ
fnp-5883	237	13	cooling	cool	VERB
fnp-5883	237	14	rate	rate	NOUN
fnp-5883	237	15	required	require	VERB
fnp-5883	237	16	for	for	ADP
fnp-5883	237	17	vitrification	vitrification	NOUN
fnp-5883	237	18	and	and	CCONJ
fnp-5883	237	19	thereby	thereby	ADV
fnp-5883	237	20	increase	increase	VERB
fnp-5883	237	21	the	the	DET
fnp-5883	237	22	vitrification	vitrification	NOUN
fnp-5883	237	23	depth	depth	NOUN
fnp-5883	237	24	.	.	PUNCT
fnp-5883	238	1	the	the	DET
fnp-5883	238	2	thinness	thinness	NOUN
fnp-5883	238	3	of	of	ADP
fnp-5883	238	4	the	the	DET
fnp-5883	238	5	samples	sample	NOUN
fnp-5883	238	6	required	require	VERB
fnp-5883	238	7	for	for	ADP
fnp-5883	238	8	cryofixation	cryofixation	NOUN
fnp-5883	238	9	is	be	AUX
fnp-5883	238	10	also	also	ADV
fnp-5883	238	11	a	a	DET
fnp-5883	238	12	challenge	challenge	NOUN
fnp-5883	238	13	for	for	ADP
fnp-5883	238	14	long	long	ADJ
fnp-5883	238	15	-	-	PUNCT
fnp-5883	238	16	term	term	NOUN
fnp-5883	238	17	brain	brain	NOUN
fnp-5883	238	18	banking	banking	NOUN
fnp-5883	238	19	,	,	PUNCT
fnp-5883	238	20	as	as	SCONJ
fnp-5883	238	21	only	only	ADV
fnp-5883	238	22	small	small	ADJ
fnp-5883	238	23	pieces	piece	NOUN
fnp-5883	238	24	of	of	ADP
fnp-5883	238	25	tissue	tissue	NOUN
fnp-5883	238	26	can	can	AUX
fnp-5883	238	27	be	be	AUX
fnp-5883	238	28	stored	store	VERB
fnp-5883	238	29	.	.	PUNCT
fnp-5883	239	1	therefore	therefore	ADV
fnp-5883	239	2	,	,	PUNCT
fnp-5883	239	3	while	while	SCONJ
fnp-5883	239	4	cryofixation	cryofixation	NOUN
fnp-5883	239	5	enables	enable	VERB
fnp-5883	239	6	optimal	optimal	ADJ
fnp-5883	239	7	ultrastructural	ultrastructural	ADJ
fnp-5883	239	8	preservation	preservation	NOUN
fnp-5883	239	9	of	of	ADP
fnp-5883	239	10	brain	brain	NOUN
fnp-5883	239	11	tissue	tissue	NOUN
fnp-5883	239	12	,	,	PUNCT
fnp-5883	239	13	it	it	PRON
fnp-5883	239	14	is	be	AUX
fnp-5883	239	15	currently	currently	ADV
fnp-5883	239	16	limited	limit	VERB
fnp-5883	239	17	to	to	ADP
fnp-5883	239	18	relatively	relatively	ADV
fnp-5883	239	19	small	small	ADJ
fnp-5883	239	20	samples	sample	NOUN
fnp-5883	239	21	,	,	PUNCT
fnp-5883	239	22	precluding	preclude	VERB
fnp-5883	239	23	its	its	PRON
fnp-5883	239	24	widespread	widespread	ADJ
fnp-5883	239	25	use	use	NOUN
fnp-5883	239	26	in	in	ADP
fnp-5883	239	27	neuroscience	neuroscience	NOUN
fnp-5883	239	28	research	research	NOUN
fnp-5883	239	29	requiring	require	VERB
fnp-5883	239	30	whole	whole	ADJ
fnp-5883	239	31	brain	brain	NOUN
fnp-5883	239	32	or	or	CCONJ
fnp-5883	239	33	thick	thick	ADJ
fnp-5883	239	34	tissue	tissue	NOUN
fnp-5883	239	35	preservation	preservation	NOUN
fnp-5883	239	36	.	.	PUNCT
fnp-5883	240	1	freezing	freeze	VERB
fnp-5883	240	2	without	without	ADP
fnp-5883	240	3	cryoprotectants	cryoprotectant	NOUN
fnp-5883	240	4	preservation	preservation	NOUN
fnp-5883	240	5	process	process	NOUN
fnp-5883	240	6	in	in	ADP
fnp-5883	240	7	the	the	DET
fnp-5883	240	8	absence	absence	NOUN
fnp-5883	240	9	of	of	ADP
fnp-5883	240	10	exogenous	exogenous	ADJ
fnp-5883	240	11	cryoprotectants	cryoprotectant	NOUN
fnp-5883	240	12	or	or	CCONJ
fnp-5883	240	13	sufficient	sufficient	ADJ
fnp-5883	240	14	cooling	cool	VERB
fnp-5883	240	15	rates	rate	NOUN
fnp-5883	240	16	for	for	ADP
fnp-5883	240	17	cryofixation	cryofixation	NOUN
fnp-5883	240	18	,	,	PUNCT
fnp-5883	240	19	ice	ice	NOUN
fnp-5883	240	20	formation	formation	NOUN
fnp-5883	240	21	during	during	ADP
fnp-5883	240	22	freezing	freezing	NOUN
fnp-5883	240	23	of	of	ADP
fnp-5883	240	24	brain	brain	NOUN
fnp-5883	240	25	tissue	tissue	NOUN
fnp-5883	240	26	is	be	AUX
fnp-5883	240	27	inevitable	inevitable	ADJ
fnp-5883	240	28	.	.	PUNCT
fnp-5883	241	1	the	the	DET
fnp-5883	241	2	critical	critical	ADJ
fnp-5883	241	3	question	question	NOUN
fnp-5883	241	4	becomes	become	VERB
fnp-5883	241	5	how	how	SCONJ
fnp-5883	241	6	much	much	ADJ
fnp-5883	241	7	damage	damage	NOUN
fnp-5883	241	8	to	to	ADP
fnp-5883	241	9	the	the	DET
fnp-5883	241	10	tissue	tissue	NOUN
fnp-5883	241	11	morphology	morphology	NOUN
fnp-5883	241	12	will	will	AUX
fnp-5883	241	13	occur	occur	VERB
fnp-5883	241	14	because	because	SCONJ
fnp-5883	241	15	of	of	ADP
fnp-5883	241	16	this	this	DET
fnp-5883	241	17	ice	ice	NOUN
fnp-5883	241	18	formation	formation	NOUN
fnp-5883	241	19	.	.	PUNCT
fnp-5883	242	1	slower	slow	ADJ
fnp-5883	242	2	cooling	cool	VERB
fnp-5883	242	3	rates	rate	NOUN
fnp-5883	242	4	allow	allow	VERB
fnp-5883	242	5	water	water	NOUN
fnp-5883	242	6	to	to	PART
fnp-5883	242	7	leave	leave	VERB
fnp-5883	242	8	cells	cell	NOUN
fnp-5883	242	9	during	during	ADP
fnp-5883	242	10	the	the	DET
fnp-5883	242	11	freezing	freezing	ADJ
fnp-5883	242	12	process	process	NOUN
fnp-5883	242	13	,	,	PUNCT
fnp-5883	242	14	causing	cause	VERB
fnp-5883	242	15	ice	ice	NOUN
fnp-5883	242	16	to	to	PART
fnp-5883	242	17	form	form	VERB
fnp-5883	242	18	predominantly	predominantly	ADV
fnp-5883	242	19	in	in	ADP
fnp-5883	242	20	the	the	DET
fnp-5883	242	21	extracellular	extracellular	ADJ
fnp-5883	242	22	space	space	NOUN
fnp-5883	242	23	.	.	PUNCT
fnp-5883	243	1	some	some	DET
fnp-5883	243	2	studies	study	NOUN
fnp-5883	243	3	have	have	AUX
fnp-5883	243	4	suggested	suggest	VERB
fnp-5883	243	5	that	that	SCONJ
fnp-5883	243	6	more	more	ADV
fnp-5883	243	7	rapid	rapid	ADJ
fnp-5883	243	8	cooling	cool	VERB
fnp-5883	243	9	rates	rate	NOUN
fnp-5883	243	10	are	be	AUX
fnp-5883	243	11	preferred	prefer	VERB
fnp-5883	243	12	when	when	SCONJ
fnp-5883	243	13	the	the	DET
fnp-5883	243	14	goal	goal	NOUN
fnp-5883	243	15	is	be	AUX
fnp-5883	243	16	to	to	PART
fnp-5883	243	17	preserve	preserve	VERB
fnp-5883	243	18	tissue	tissue	NOUN
fnp-5883	243	19	morphology	morphology	NOUN
fnp-5883	243	20	,	,	PUNCT
fnp-5883	243	21	particularly	particularly	ADV
fnp-5883	243	22	in	in	ADP
fnp-5883	243	23	specimens	specimen	NOUN
fnp-5883	243	24	with	with	ADP
fnp-5883	243	25	complex	complex	ADJ
fnp-5883	243	26	microanatomical	microanatomical	ADJ
fnp-5883	243	27	structures	structure	NOUN
fnp-5883	243	28	like	like	ADP
fnp-5883	243	29	brain	brain	NOUN
fnp-5883	243	30	tissue	tissue	NOUN
fnp-5883	243	31	.	.	PUNCT
fnp-5883	244	1	this	this	PRON
fnp-5883	244	2	is	be	AUX
fnp-5883	244	3	because	because	SCONJ
fnp-5883	244	4	extracellular	extracellular	ADJ
fnp-5883	244	5	ice	ice	NOUN
fnp-5883	244	6	,	,	PUNCT
fnp-5883	244	7	while	while	SCONJ
fnp-5883	244	8	less	less	ADV
fnp-5883	244	9	damaging	damaging	ADJ
fnp-5883	244	10	to	to	ADP
fnp-5883	244	11	cell	cell	NOUN
fnp-5883	244	12	viability	viability	NOUN
fnp-5883	244	13	because	because	SCONJ
fnp-5883	244	14	it	it	PRON
fnp-5883	244	15	helps	help	VERB
fnp-5883	244	16	avoid	avoid	VERB
fnp-5883	244	17	intracellular	intracellular	ADJ
fnp-5883	244	18	ice	ice	NOUN
fnp-5883	244	19	,	,	PUNCT
fnp-5883	244	20	can	can	AUX
fnp-5883	244	21	severely	severely	ADV
fnp-5883	244	22	disrupt	disrupt	VERB
fnp-5883	244	23	tissue	tissue	NOUN
fnp-5883	244	24	structures	structure	NOUN
fnp-5883	244	25	,	,	PUNCT
fnp-5883	244	26	such	such	ADJ
fnp-5883	244	27	as	as	ADP
fnp-5883	244	28	cellular	cellular	ADJ
fnp-5883	244	29	processes	process	NOUN
fnp-5883	244	30	and	and	CCONJ
fnp-5883	244	31	synaptic	synaptic	ADJ
fnp-5883	244	32	connections	connection	NOUN
fnp-5883	244	33	(	(	PUNCT
fnp-5883	244	34	pegg	pegg	NOUN
fnp-5883	244	35	,	,	PUNCT
fnp-5883	244	36	2007	2007	NUM
fnp-5883	244	37	)	)	PUNCT
fnp-5883	244	38	.	.	PUNCT
fnp-5883	245	1	rapid	rapid	ADJ
fnp-5883	245	2	cooling	cooling	NOUN
fnp-5883	245	3	minimizes	minimize	VERB
fnp-5883	245	4	the	the	DET
fnp-5883	245	5	time	time	NOUN
fnp-5883	245	6	available	available	ADJ
fnp-5883	245	7	for	for	ADP
fnp-5883	245	8	ice	ice	NOUN
fnp-5883	245	9	crystal	crystal	NOUN
fnp-5883	245	10	nucleation	nucleation	NOUN
fnp-5883	245	11	,	,	PUNCT
fnp-5883	245	12	growth	growth	NOUN
fnp-5883	245	13	,	,	PUNCT
fnp-5883	245	14	and	and	CCONJ
fnp-5883	245	15	rearrangement	rearrangement	NOUN
fnp-5883	245	16	,	,	PUNCT
fnp-5883	245	17	which	which	PRON
fnp-5883	245	18	may	may	AUX
fnp-5883	245	19	cause	cause	VERB
fnp-5883	245	20	less	less	ADJ
fnp-5883	245	21	damage	damage	NOUN
fnp-5883	245	22	to	to	ADP
fnp-5883	245	23	cellular	cellular	ADJ
fnp-5883	245	24	morphology	morphology	NOUN
fnp-5883	245	25	(	(	PUNCT
fnp-5883	245	26	rosene	rosene	NOUN
fnp-5883	245	27	et	et	PROPN
fnp-5883	245	28	al	al	PROPN
fnp-5883	245	29	.	.	PROPN
fnp-5883	245	30	,	,	PUNCT
fnp-5883	245	31	1986	1986	NUM
fnp-5883	245	32	)	)	PUNCT
fnp-5883	245	33	.	.	PUNCT
fnp-5883	246	1	therefore	therefore	ADV
fnp-5883	246	2	,	,	PUNCT
fnp-5883	246	3	in	in	ADP
fnp-5883	246	4	the	the	DET
fnp-5883	246	5	context	context	NOUN
fnp-5883	246	6	of	of	ADP
fnp-5883	246	7	freezing	freeze	VERB
fnp-5883	246	8	brain	brain	NOUN
fnp-5883	246	9	tissue	tissue	NOUN
fnp-5883	246	10	without	without	ADP
fnp-5883	246	11	cryoprotectants	cryoprotectant	NOUN
fnp-5883	246	12	,	,	PUNCT
fnp-5883	246	13	the	the	DET
fnp-5883	246	14	most	most	ADV
fnp-5883	246	15	common	common	ADJ
fnp-5883	246	16	approach	approach	NOUN
fnp-5883	246	17	used	use	VERB
fnp-5883	246	18	by	by	ADP
fnp-5883	246	19	investigators	investigator	NOUN
fnp-5883	246	20	with	with	ADP
fnp-5883	246	21	the	the	DET
fnp-5883	246	22	goal	goal	NOUN
fnp-5883	246	23	of	of	ADP
fnp-5883	246	24	morphological	morphological	ADJ
fnp-5883	246	25	preservation	preservation	NOUN
fnp-5883	246	26	has	have	AUX
fnp-5883	246	27	been	be	AUX
fnp-5883	246	28	to	to	PART
fnp-5883	246	29	maximize	maximize	VERB
fnp-5883	246	30	the	the	DET
fnp-5883	246	31	cooling	cool	VERB
fnp-5883	246	32	rate	rate	NOUN
fnp-5883	246	33	.	.	PUNCT
fnp-5883	247	1	the	the	DET
fnp-5883	247	2	main	main	ADJ
fnp-5883	247	3	challenge	challenge	NOUN
fnp-5883	247	4	lies	lie	VERB
fnp-5883	247	5	in	in	ADP
fnp-5883	247	6	achieving	achieve	VERB
fnp-5883	247	7	sufficiently	sufficiently	ADV
fnp-5883	247	8	rapid	rapid	ADJ
fnp-5883	247	9	cooling	cooling	NOUN
fnp-5883	247	10	throughout	throughout	ADP
fnp-5883	247	11	the	the	DET
fnp-5883	247	12	entire	entire	ADJ
fnp-5883	247	13	tissue	tissue	NOUN
fnp-5883	247	14	volume	volume	NOUN
fnp-5883	247	15	,	,	PUNCT
fnp-5883	247	16	particularly	particularly	ADV
fnp-5883	247	17	for	for	ADP
fnp-5883	247	18	larger	large	ADJ
fnp-5883	247	19	specimens	specimen	NOUN
fnp-5883	247	20	.	.	PUNCT
fnp-5883	248	1	effects	effect	NOUN
fnp-5883	248	2	on	on	ADP
fnp-5883	248	3	tissue	tissue	NOUN
fnp-5883	248	4	morphology	morphology	NOUN
fnp-5883	248	5	there	there	PRON
fnp-5883	248	6	were	be	VERB
fnp-5883	248	7	numerous	numerous	ADJ
fnp-5883	248	8	methods	method	NOUN
fnp-5883	248	9	described	describe	VERB
fnp-5883	248	10	for	for	ADP
fnp-5883	248	11	freezing	freeze	VERB
fnp-5883	248	12	brain	brain	NOUN
fnp-5883	248	13	and	and	CCONJ
fnp-5883	248	14	other	other	ADJ
fnp-5883	248	15	nervous	nervous	ADJ
fnp-5883	248	16	tissue	tissue	NOUN
fnp-5883	248	17	in	in	ADP
fnp-5883	248	18	the	the	DET
fnp-5883	248	19	studies	study	NOUN
fnp-5883	248	20	we	we	PRON
fnp-5883	248	21	identified	identify	VERB
fnp-5883	248	22	.	.	PUNCT
fnp-5883	249	1	these	these	PRON
fnp-5883	249	2	included	include	VERB
fnp-5883	249	3	(	(	PUNCT
fnp-5883	249	4	a	a	X
fnp-5883	249	5	)	)	PUNCT
fnp-5883	249	6	placing	place	VERB
fnp-5883	249	7	the	the	DET
fnp-5883	249	8	specimen	speciman	NOUN
fnp-5883	249	9	in	in	ADP
fnp-5883	249	10	a	a	DET
fnp-5883	249	11	freezer	freezer	NOUN
fnp-5883	249	12	,	,	PUNCT
fnp-5883	249	13	such	such	ADJ
fnp-5883	249	14	as	as	ADP
fnp-5883	249	15	one	one	NUM
fnp-5883	249	16	set	set	VERB
fnp-5883	249	17	to	to	ADP
fnp-5883	249	18	‑80	‑80	VERB
fnp-5883	249	19	 	 	SPACE
fnp-5883	249	20	°	°	ADP
fnp-5883	249	21	c	c	NOUN
fnp-5883	249	22	or	or	CCONJ
fnp-5883	249	23	‑20	‑20	PRON
fnp-5883	249	24	 	 	SPACE
fnp-5883	249	25	°	°	ADP
fnp-5883	249	26	c	c	PROPN
fnp-5883	249	27	(	(	PUNCT
fnp-5883	249	28	vonsattel	vonsattel	NOUN
fnp-5883	249	29	et	et	PROPN
fnp-5883	249	30	al	al	PROPN
fnp-5883	249	31	.	.	PROPN
fnp-5883	249	32	,	,	PUNCT
fnp-5883	249	33	1995	1995	NUM
fnp-5883	249	34	;	;	PUNCT
fnp-5883	249	35	kagan	kagan	PROPN
fnp-5883	249	36	and	and	CCONJ
fnp-5883	249	37	viner	viner	NOUN
fnp-5883	249	38	,	,	PUNCT
fnp-5883	249	39	2022	2022	NUM
fnp-5883	249	40	)	)	PUNCT
fnp-5883	249	41	,	,	PUNCT
fnp-5883	249	42	(	(	PUNCT
fnp-5883	249	43	b	b	X
fnp-5883	249	44	)	)	PUNCT
fnp-5883	249	45	placing	place	VERB
fnp-5883	249	46	the	the	DET
fnp-5883	249	47	tissue	tissue	NOUN
fnp-5883	249	48	in	in	ADP
fnp-5883	249	49	or	or	CCONJ
fnp-5883	249	50	between	between	ADP
fnp-5883	249	51	a	a	DET
fnp-5883	249	52	form	form	NOUN
fnp-5883	249	53	of	of	ADP
fnp-5883	249	54	dry	dry	ADJ
fnp-5883	249	55	ice	ice	NOUN
fnp-5883	249	56	at	at	ADP
fnp-5883	249	57	‑78.5	‑78.5	PROPN
fnp-5883	249	58	 	 	SPACE
fnp-5883	249	59	°	°	PROPN
fnp-5883	249	60	c	c	PROPN
fnp-5883	249	61	(	(	PUNCT
fnp-5883	249	62	beach	beach	PROPN
fnp-5883	249	63	et	et	PROPN
fnp-5883	249	64	al	al	PROPN
fnp-5883	249	65	.	.	PROPN
fnp-5883	249	66	,	,	PUNCT
fnp-5883	249	67	2008	2008	NUM
fnp-5883	249	68	)	)	PUNCT
fnp-5883	249	69	,	,	PUNCT
fnp-5883	249	70	(	(	PUNCT
fnp-5883	249	71	c	c	X
fnp-5883	249	72	)	)	PUNCT
fnp-5883	249	73	immersing	immerse	VERB
fnp-5883	249	74	the	the	DET
fnp-5883	249	75	specimen	speciman	NOUN
fnp-5883	249	76	in	in	ADP
fnp-5883	249	77	liquid	liquid	ADJ
fnp-5883	249	78	nitrogen	nitrogen	NOUN
fnp-5883	249	79	(	(	PUNCT
fnp-5883	249	80	ulfig	ulfig	NOUN
fnp-5883	249	81	et	et	PROPN
fnp-5883	249	82	al	al	PROPN
fnp-5883	249	83	.	.	PROPN
fnp-5883	249	84	,	,	PUNCT
fnp-5883	249	85	1998	1998	NUM
fnp-5883	249	86	)	)	PUNCT
fnp-5883	249	87	,	,	PUNCT
fnp-5883	249	88	(	(	PUNCT
fnp-5883	249	89	d	d	X
fnp-5883	249	90	)	)	PUNCT
fnp-5883	249	91	immersing	immerse	VERB
fnp-5883	249	92	the	the	DET
fnp-5883	249	93	specimen	speciman	NOUN
fnp-5883	249	94	in	in	ADP
fnp-5883	249	95	liquid	liquid	ADJ
fnp-5883	249	96	nitrogen	nitrogen	NOUN
fnp-5883	249	97	vapor	vapor	NOUN
fnp-5883	249	98	(	(	PUNCT
fnp-5883	249	99	davis	davis	PROPN
fnp-5883	249	100	et	et	PROPN
fnp-5883	249	101	al	al	PROPN
fnp-5883	249	102	.	.	PROPN
fnp-5883	249	103	,	,	PUNCT
fnp-5883	249	104	2019	2019	NUM
fnp-5883	249	105	)	)	PUNCT
fnp-5883	249	106	,	,	PUNCT
fnp-5883	249	107	(	(	PUNCT
fnp-5883	249	108	e	e	X
fnp-5883	249	109	)	)	PUNCT
fnp-5883	249	110	immersing	immerse	VERB
fnp-5883	249	111	the	the	DET
fnp-5883	249	112	specimen	speciman	NOUN
fnp-5883	249	113	in	in	ADP
fnp-5883	249	114	isopentane	isopentane	NOUN
fnp-5883	249	115	that	that	PRON
fnp-5883	249	116	has	have	AUX
fnp-5883	249	117	been	be	AUX
fnp-5883	249	118	pre	pre	VERB
fnp-5883	249	119	-	-	VERB
fnp-5883	249	120	cooled	cooled	ADJ
fnp-5883	249	121	with	with	ADP
fnp-5883	249	122	a	a	DET
fnp-5883	249	123	different	different	ADJ
fnp-5883	249	124	cryogen	cryogen	NOUN
fnp-5883	249	125	,	,	PUNCT
fnp-5883	249	126	such	such	ADJ
fnp-5883	249	127	as	as	ADP
fnp-5883	249	128	liquid	liquid	ADJ
fnp-5883	249	129	nitrogen	nitrogen	NOUN
fnp-5883	249	130	or	or	CCONJ
fnp-5883	249	131	dry	dry	ADJ
fnp-5883	249	132	ice	ice	NOUN
fnp-5883	249	133	(	(	PUNCT
fnp-5883	249	134	priemer	priemer	NOUN
fnp-5883	249	135	et	et	PROPN
fnp-5883	249	136	al	al	PROPN
fnp-5883	249	137	.	.	PROPN
fnp-5883	249	138	,	,	PUNCT
fnp-5883	249	139	2023	2023	NUM
fnp-5883	249	140	)	)	PUNCT
fnp-5883	249	141	,	,	PUNCT
fnp-5883	249	142	or	or	CCONJ
fnp-5883	249	143	(	(	PUNCT
fnp-5883	249	144	f	f	X
fnp-5883	249	145	)	)	PUNCT
fnp-5883	249	146	freezing	freeze	VERB
fnp-5883	249	147	the	the	DET
fnp-5883	249	148	specimen	speciman	NOUN
fnp-5883	249	149	against	against	ADP
fnp-5883	249	150	metal	metal	NOUN
fnp-5883	249	151	surfaces	surface	NOUN
fnp-5883	249	152	that	that	PRON
fnp-5883	249	153	have	have	AUX
fnp-5883	249	154	been	be	AUX
fnp-5883	249	155	pre	pre	VERB
fnp-5883	249	156	-	-	VERB
fnp-5883	249	157	cooled	cooled	ADJ
fnp-5883	249	158	with	with	ADP
fnp-5883	249	159	a	a	DET
fnp-5883	249	160	different	different	ADJ
fnp-5883	249	161	method	method	NOUN
fnp-5883	249	162	,	,	PUNCT
fnp-5883	249	163	such	such	ADJ
fnp-5883	249	164	as	as	ADP
fnp-5883	249	165	liquid	liquid	ADJ
fnp-5883	249	166	nitrogen	nitrogen	NOUN
fnp-5883	249	167	vapor	vapor	NOUN
fnp-5883	249	168	or	or	CCONJ
fnp-5883	249	169	a	a	DET
fnp-5883	249	170	‑80	‑80	NOUN
fnp-5883	249	171	 	 	SPACE
fnp-5883	249	172	°	°	PROPN
fnp-5883	249	173	c	c	NOUN
fnp-5883	249	174	freezer	freezer	NOUN
fnp-5883	249	175	(	(	PUNCT
fnp-5883	249	176	vonsattel	vonsattel	NOUN
fnp-5883	249	177	et	et	PROPN
fnp-5883	249	178	al	al	PROPN
fnp-5883	249	179	.	.	PROPN
fnp-5883	249	180	,	,	PUNCT
fnp-5883	249	181	2008	2008	NUM
fnp-5883	249	182	)	)	PUNCT
fnp-5883	249	183	.	.	PUNCT
fnp-5883	250	1	the	the	DET
fnp-5883	250	2	efficacy	efficacy	NOUN
fnp-5883	250	3	of	of	ADP
fnp-5883	250	4	these	these	DET
fnp-5883	250	5	freezing	freezing	ADJ
fnp-5883	250	6	methods	method	NOUN
fnp-5883	250	7	varied	vary	VERB
fnp-5883	250	8	considerably	considerably	ADV
fnp-5883	250	9	in	in	ADP
fnp-5883	250	10	the	the	DET
fnp-5883	250	11	studies	study	NOUN
fnp-5883	250	12	we	we	PRON
fnp-5883	250	13	identified	identify	VERB
fnp-5883	250	14	(	(	PUNCT
fnp-5883	250	15	table	table	NOUN
fnp-5883	250	16	 	 	SPACE
fnp-5883	250	17	3	3	NUM
fnp-5883	250	18	;	;	PUNCT
fnp-5883	250	19	supplementary	supplementary	ADJ
fnp-5883	250	20	file	file	NOUN
fnp-5883	250	21	 	 	SPACE
fnp-5883	250	22	4	4	NUM
fnp-5883	250	23	)	)	PUNCT
fnp-5883	250	24	(	(	PUNCT
fnp-5883	250	25	rosene	rosene	NOUN
fnp-5883	250	26	and	and	CCONJ
fnp-5883	250	27	rhodes	rhode	NOUN
fnp-5883	250	28	,	,	PUNCT
fnp-5883	250	29	1990	1990	NUM
fnp-5883	250	30	)	)	PUNCT
fnp-5883	250	31	.	.	PUNCT
fnp-5883	251	1	one	one	NUM
fnp-5883	251	2	study	study	NOUN
fnp-5883	251	3	found	find	VERB
fnp-5883	251	4	that	that	SCONJ
fnp-5883	251	5	freezing	freeze	VERB
fnp-5883	251	6	human	human	ADJ
fnp-5883	251	7	brain	brain	NOUN
fnp-5883	251	8	slices	slice	NOUN
fnp-5883	251	9	either	either	CCONJ
fnp-5883	251	10	with	with	ADP
fnp-5883	251	11	dry	dry	ADJ
fnp-5883	251	12	ice	ice	NOUN
fnp-5883	251	13	snow	snow	NOUN
fnp-5883	251	14	,	,	PUNCT
fnp-5883	251	15	isopentane	isopentane	ADJ
fnp-5883	251	16	pre	pre	VERB
fnp-5883	251	17	-	-	ADJ
fnp-5883	251	18	cooled	cooled	ADJ
fnp-5883	251	19	in	in	ADP
fnp-5883	251	20	liquid	liquid	ADJ
fnp-5883	251	21	nitrogen	nitrogen	NOUN
fnp-5883	251	22	,	,	PUNCT
fnp-5883	251	23	copper	copper	NOUN
fnp-5883	251	24	plates	plate	NOUN
fnp-5883	251	25	pre	pre	VERB
fnp-5883	251	26	-	-	ADJ
fnp-5883	251	27	cooled	cool	VERB
fnp-5883	251	28	to	to	ADP
fnp-5883	251	29	‑80	‑80	NOUN
fnp-5883	251	30	 	 	SPACE
fnp-5883	251	31	°	°	ADP
fnp-5883	251	32	c	c	NOUN
fnp-5883	251	33	,	,	PUNCT
fnp-5883	251	34	or	or	CCONJ
fnp-5883	251	35	placing	place	VERB
fnp-5883	251	36	it	it	PRON
fnp-5883	251	37	in	in	ADP
fnp-5883	251	38	a	a	DET
fnp-5883	251	39	‑20	‑20	ADJ
fnp-5883	251	40	 	 	SPACE
fnp-5883	251	41	°	°	NOUN
fnp-5883	251	42	c	c	NOUN
fnp-5883	251	43	freezer	freezer	NOUN
fnp-5883	251	44	after	after	ADP
fnp-5883	251	45	embedding	embed	VERB
fnp-5883	251	46	the	the	DET
fnp-5883	251	47	tissue	tissue	NOUN
fnp-5883	251	48	in	in	ADP
fnp-5883	251	49	a	a	DET
fnp-5883	251	50	cryo	cryo	NOUN
fnp-5883	251	51	-	-	PUNCT
fnp-5883	251	52	conductive	conductive	ADJ
fnp-5883	251	53	mixture	mixture	NOUN
fnp-5883	251	54	of	of	ADP
fnp-5883	251	55	chemicals	chemical	NOUN
fnp-5883	251	56	called	call	VERB
fnp-5883	251	57	optimal	optimal	ADJ
fnp-5883	251	58	cutting	cutting	NOUN
fnp-5883	251	59	temperature	temperature	NOUN
fnp-5883	251	60	(	(	PUNCT
fnp-5883	251	61	oct	oct	NOUN
fnp-5883	251	62	)	)	PUNCT
fnp-5883	251	63	all	all	PRON
fnp-5883	251	64	provided	provide	VERB
fnp-5883	251	65	good	good	ADJ
fnp-5883	251	66	results	result	NOUN
fnp-5883	251	67	,	,	PUNCT
fnp-5883	251	68	with	with	ADP
fnp-5883	251	69	well	well	ADV
fnp-5883	251	70	-	-	PUNCT
fnp-5883	251	71	preserved	preserve	VERB
fnp-5883	251	72	brain	brain	NOUN
fnp-5883	251	73	tissue	tissue	NOUN
fnp-5883	251	74	architecture	architecture	NOUN
fnp-5883	251	75	and	and	CCONJ
fnp-5883	251	76	cell	cell	NOUN
fnp-5883	251	77	morphology	morphology	NOUN
fnp-5883	251	78	as	as	SCONJ
fnp-5883	251	79	seen	see	VERB
fnp-5883	251	80	on	on	ADP
fnp-5883	251	81	light	light	ADJ
fnp-5883	251	82	microscopy	microscopy	NOUN
fnp-5883	251	83	.	.	PUNCT
fnp-5883	252	1	in	in	ADP
fnp-5883	252	2	contrast	contrast	NOUN
fnp-5883	252	3	,	,	PUNCT
fnp-5883	252	4	samples	sample	NOUN
fnp-5883	252	5	directly	directly	ADV
fnp-5883	252	6	frozen	freeze	VERB
fnp-5883	252	7	in	in	ADP
fnp-5883	252	8	a	a	DET
fnp-5883	252	9	‑80	‑80	NOUN
fnp-5883	252	10	 	 	SPACE
fnp-5883	252	11	°	°	ADP
fnp-5883	252	12	c	c	NOUN
fnp-5883	252	13	freezer	freezer	NOUN
fnp-5883	252	14	or	or	CCONJ
fnp-5883	252	15	immersed	immerse	VERB
fnp-5883	252	16	in	in	ADP
fnp-5883	252	17	liquid	liquid	ADJ
fnp-5883	252	18	nitrogen	nitrogen	NOUN
fnp-5883	252	19	showed	show	VERB
fnp-5883	252	20	high	high	ADJ
fnp-5883	252	21	levels	level	NOUN
fnp-5883	252	22	of	of	ADP
fnp-5883	252	23	ice	ice	NOUN
fnp-5883	252	24	crystal	crystal	NOUN
fnp-5883	252	25	artifacts	artifact	NOUN
fnp-5883	252	26	(	(	PUNCT
fnp-5883	252	27	meyronet	meyronet	NOUN
fnp-5883	252	28	et	et	PROPN
fnp-5883	252	29	al	al	PROPN
fnp-5883	252	30	.	.	PROPN
fnp-5883	252	31	,	,	PUNCT
fnp-5883	252	32	2015	2015	NUM
fnp-5883	252	33	)	)	PUNCT
fnp-5883	252	34	.	.	PUNCT
fnp-5883	253	1	a	a	DET
fnp-5883	253	2	particularly	particularly	ADV
fnp-5883	253	3	important	important	ADJ
fnp-5883	253	4	clinical	clinical	ADJ
fnp-5883	253	5	application	application	NOUN
fnp-5883	253	6	of	of	ADP
fnp-5883	253	7	freezing	freezing	ADJ
fnp-5883	253	8	methods	method	NOUN
fnp-5883	253	9	is	be	AUX
fnp-5883	253	10	the	the	DET
fnp-5883	253	11	use	use	NOUN
fnp-5883	253	12	of	of	ADP
fnp-5883	253	13	frozen	frozen	ADJ
fnp-5883	253	14	sections	section	NOUN
fnp-5883	253	15	.	.	PUNCT
fnp-5883	254	1	one	one	NUM
fnp-5883	254	2	study	study	NOUN
fnp-5883	254	3	found	find	VERB
fnp-5883	254	4	that	that	SCONJ
fnp-5883	254	5	snap	snap	VERB
fnp-5883	254	6	freezing	freeze	VERB
fnp-5883	254	7	biopsy	biopsy	NOUN
fnp-5883	254	8	samples	sample	NOUN
fnp-5883	254	9	in	in	ADP
fnp-5883	254	10	dry	dry	ADJ
fnp-5883	254	11	ice	ice	NOUN
fnp-5883	254	12	-	-	PUNCT
fnp-5883	254	13	cooled	cool	VERB
fnp-5883	254	14	isopentane	isopentane	NOUN
fnp-5883	254	15	led	lead	VERB
fnp-5883	254	16	to	to	ADP
fnp-5883	254	17	considerably	considerably	ADV
fnp-5883	254	18	better	well	ADJ
fnp-5883	254	19	histology	histology	NOUN
fnp-5883	254	20	preservation	preservation	NOUN
fnp-5883	254	21	and	and	CCONJ
fnp-5883	254	22	less	less	ADJ
fnp-5883	254	23	ice	ice	NOUN
fnp-5883	254	24	formation	formation	NOUN
fnp-5883	254	25	compared	compare	VERB
fnp-5883	254	26	to	to	ADP
fnp-5883	254	27	freezing	freeze	VERB
fnp-5883	254	28	samples	sample	NOUN
fnp-5883	254	29	by	by	ADP
fnp-5883	254	30	placing	place	VERB
fnp-5883	254	31	them	they	PRON
fnp-5883	254	32	on	on	ADP
fnp-5883	254	33	the	the	DET
fnp-5883	254	34	freezing	freezing	ADJ
fnp-5883	254	35	shelf	shelf	NOUN
fnp-5883	254	36	within	within	ADP
fnp-5883	254	37	the	the	DET
fnp-5883	254	38	cryostat	cryostat	NOUN
fnp-5883	254	39	at	at	ADP
fnp-5883	254	40	a	a	DET
fnp-5883	254	41	temperature	temperature	NOUN
fnp-5883	254	42	of	of	ADP
fnp-5883	254	43	around	around	ADP
fnp-5883	254	44	‑20	‑20	ADJ
fnp-5883	254	45	 	 	SPACE
fnp-5883	254	46	°	°	ADP
fnp-5883	254	47	c	c	NOUN
fnp-5883	254	48	(	(	PUNCT
fnp-5883	254	49	priemer	priemer	NOUN
fnp-5883	254	50	et	et	PROPN
fnp-5883	254	51	al	al	PROPN
fnp-5883	254	52	.	.	PROPN
fnp-5883	254	53	,	,	PUNCT
fnp-5883	254	54	2023	2023	NUM
fnp-5883	254	55	)	)	PUNCT
fnp-5883	254	56	.	.	PUNCT
fnp-5883	255	1	sample	sample	NOUN
fnp-5883	255	2	type	type	NOUN
fnp-5883	255	3	(	(	PUNCT
fnp-5883	255	4	study	study	NOUN
fnp-5883	255	5	)	)	PUNCT
fnp-5883	255	6	cooling	cool	VERB
fnp-5883	255	7	method	method	NOUN
fnp-5883	255	8	visualization	visualization	NOUN
fnp-5883	255	9	method	method	NOUN
fnp-5883	255	10	key	key	ADJ
fnp-5883	255	11	findings	finding	NOUN
fnp-5883	255	12	human	human	ADJ
fnp-5883	255	13	frontal	frontal	ADJ
fnp-5883	255	14	white	white	ADJ
fnp-5883	255	15	matter	matter	NOUN
fnp-5883	255	16	samples	sample	NOUN
fnp-5883	255	17	(	(	PUNCT
fnp-5883	255	18	ansari	ansari	PROPN
fnp-5883	255	19	et	et	PROPN
fnp-5883	255	20	al	al	PROPN
fnp-5883	255	21	.	.	PROPN
fnp-5883	255	22	,	,	PUNCT
fnp-5883	255	23	1976	1976	NUM
fnp-5883	255	24	)	)	PUNCT
fnp-5883	255	25	frozen	freeze	VERB
fnp-5883	255	26	in	in	ADP
fnp-5883	255	27	a	a	DET
fnp-5883	255	28	dry	dry	ADJ
fnp-5883	255	29	ice	ice	NOUN
fnp-5883	255	30	-	-	PUNCT
fnp-5883	255	31	acetone	acetone	NOUN
fnp-5883	255	32	bath	bath	NOUN
fnp-5883	255	33	,	,	PUNCT
fnp-5883	255	34	stored	store	VERB
fnp-5883	255	35	at	at	ADP
fnp-5883	255	36	‑80	‑80	NOUN
fnp-5883	255	37	 	 	SPACE
fnp-5883	255	38	°	°	NOUN
fnp-5883	255	39	c	c	NOUN
fnp-5883	255	40	thawed	thaw	VERB
fnp-5883	255	41	in	in	ADP
fnp-5883	255	42	fixative	fixative	ADJ
fnp-5883	255	43	,	,	PUNCT
fnp-5883	255	44	electron	electron	NOUN
fnp-5883	255	45	microscopy	microscopy	NOUN
fnp-5883	255	46	myelin	myelin	NOUN
fnp-5883	255	47	has	have	VERB
fnp-5883	255	48	separation	separation	NOUN
fnp-5883	255	49	of	of	ADP
fnp-5883	255	50	lamellae	lamellae	NOUN
fnp-5883	255	51	and	and	CCONJ
fnp-5883	255	52	numerous	numerous	ADJ
fnp-5883	255	53	regions	region	NOUN
fnp-5883	255	54	with	with	ADP
fnp-5883	255	55	disruption	disruption	NOUN
fnp-5883	255	56	of	of	ADP
fnp-5883	255	57	the	the	DET
fnp-5883	255	58	lamellae	lamellae	NOUN
fnp-5883	255	59	mouse	mouse	NOUN
fnp-5883	255	60	sciatic	sciatic	ADJ
fnp-5883	255	61	nerves	nerve	NOUN
fnp-5883	255	62	(	(	PUNCT
fnp-5883	255	63	kirschner	kirschner	PROPN
fnp-5883	255	64	et	et	PROPN
fnp-5883	255	65	al	al	PROPN
fnp-5883	255	66	.	.	PROPN
fnp-5883	255	67	,	,	PUNCT
fnp-5883	255	68	1979	1979	NUM
fnp-5883	255	69	)	)	PUNCT
fnp-5883	255	70	frozen	freeze	VERB
fnp-5883	255	71	in	in	ADP
fnp-5883	255	72	a	a	DET
fnp-5883	255	73	freon	freon	NOUN
fnp-5883	255	74	22	22	NUM
fnp-5883	255	75	slush	slush	NOUN
fnp-5883	255	76	cooled	cool	VERB
fnp-5883	255	77	with	with	ADP
fnp-5883	255	78	liquid	liquid	ADJ
fnp-5883	255	79	nitrogen	nitrogen	NOUN
fnp-5883	255	80	x	x	NOUN
fnp-5883	255	81	-	-	NOUN
fnp-5883	255	82	ray	ray	NOUN
fnp-5883	255	83	diffraction	diffraction	NOUN
fnp-5883	255	84	,	,	PUNCT
fnp-5883	255	85	freeze	freeze	NOUN
fnp-5883	255	86	-	-	PUNCT
fnp-5883	255	87	fracture	fracture	NOUN
fnp-5883	255	88	electron	electron	NOUN
fnp-5883	255	89	microscopy	microscopy	NOUN
fnp-5883	255	90	most	most	ADJ
fnp-5883	255	91	membrane	membrane	NOUN
fnp-5883	255	92	arrays	array	NOUN
fnp-5883	255	93	in	in	ADP
fnp-5883	255	94	myelin	myelin	ADJ
fnp-5883	255	95	sheaths	sheath	NOUN
fnp-5883	255	96	are	be	AUX
fnp-5883	255	97	distorted	distort	VERB
fnp-5883	255	98	,	,	PUNCT
fnp-5883	255	99	but	but	CCONJ
fnp-5883	255	100	a	a	DET
fnp-5883	255	101	few	few	ADJ
fnp-5883	255	102	regions	region	NOUN
fnp-5883	255	103	that	that	PRON
fnp-5883	255	104	are	be	AUX
fnp-5883	255	105	normally	normally	ADV
fnp-5883	255	106	ordered	order	VERB
fnp-5883	255	107	remain	remain	VERB
fnp-5883	255	108	1	1	NUM
fnp-5883	255	109	 	 	SPACE
fnp-5883	255	110	cm	cm	NOUN
fnp-5883	255	111	thick	thick	ADJ
fnp-5883	255	112	dog	dog	NOUN
fnp-5883	255	113	hemisphere	hemisphere	ADJ
fnp-5883	255	114	sections	section	NOUN
fnp-5883	255	115	(	(	PUNCT
fnp-5883	255	116	baraibar	baraibar	NOUN
fnp-5883	255	117	and	and	CCONJ
fnp-5883	255	118	schoning	schoning	NOUN
fnp-5883	255	119	,	,	PUNCT
fnp-5883	255	120	1985	1985	NUM
fnp-5883	255	121	)	)	PUNCT
fnp-5883	255	122	frozen	freeze	VERB
fnp-5883	255	123	to	to	PART
fnp-5883	255	124	‑18	‑18	VERB
fnp-5883	255	125	 	 	SPACE
fnp-5883	255	126	°	°	NOUN
fnp-5883	255	127	c	c	NOUN
fnp-5883	255	128	,	,	PUNCT
fnp-5883	255	129	stored	store	VERB
fnp-5883	255	130	for	for	ADP
fnp-5883	255	131	2	2	NUM
fnp-5883	255	132	days	day	NOUN
fnp-5883	255	133	fixed	fix	VERB
fnp-5883	255	134	at	at	ADP
fnp-5883	255	135	room	room	NOUN
fnp-5883	255	136	temperature	temperature	NOUN
fnp-5883	255	137	,	,	PUNCT
fnp-5883	255	138	light	light	ADJ
fnp-5883	255	139	microscopy	microscopy	NOUN
fnp-5883	255	140	with	with	ADP
fnp-5883	255	141	h&e	h&e	PROPN
fnp-5883	255	142	staining	stain	VERB
fnp-5883	255	143	large	large	ADJ
fnp-5883	255	144	vacuoles	vacuole	NOUN
fnp-5883	255	145	suggestive	suggestive	ADJ
fnp-5883	255	146	of	of	ADP
fnp-5883	255	147	ice	ice	NOUN
fnp-5883	255	148	formation	formation	NOUN
fnp-5883	255	149	,	,	PUNCT
fnp-5883	255	150	lack	lack	NOUN
fnp-5883	255	151	of	of	ADP
fnp-5883	255	152	detail	detail	NOUN
fnp-5883	255	153	in	in	ADP
fnp-5883	255	154	cell	cell	NOUN
fnp-5883	255	155	outlines	outline	NOUN
fnp-5883	255	156	,	,	PUNCT
fnp-5883	255	157	hemolysis	hemolysis	NOUN
fnp-5883	255	158	,	,	PUNCT
fnp-5883	255	159	increased	increase	VERB
fnp-5883	255	160	eosin	eosin	NOUN
fnp-5883	255	161	staining	stain	VERB
fnp-5883	255	162	1	1	NUM
fnp-5883	255	163	 	 	SPACE
fnp-5883	255	164	cm	cm	NOUN
fnp-5883	255	165	thick	thick	ADJ
fnp-5883	255	166	human	human	ADJ
fnp-5883	255	167	brain	brain	NOUN
fnp-5883	255	168	sections	section	NOUN
fnp-5883	255	169	(	(	PUNCT
fnp-5883	255	170	nochlin	nochlin	PROPN
fnp-5883	255	171	et	et	PROPN
fnp-5883	255	172	al	al	PROPN
fnp-5883	255	173	.	.	PROPN
fnp-5883	255	174	,	,	PUNCT
fnp-5883	255	175	1993	1993	NUM
fnp-5883	255	176	)	)	PUNCT
fnp-5883	255	177	frozen	freeze	VERB
fnp-5883	255	178	between	between	ADP
fnp-5883	255	179	aluminum	aluminum	NOUN
fnp-5883	255	180	plates	plate	NOUN
fnp-5883	255	181	cooled	cool	VERB
fnp-5883	255	182	with	with	ADP
fnp-5883	255	183	dry	dry	ADJ
fnp-5883	255	184	ice	ice	NOUN
fnp-5883	255	185	immersion	immersion	NOUN
fnp-5883	255	186	fixed	fix	VERB
fnp-5883	255	187	,	,	PUNCT
fnp-5883	255	188	electron	electron	NOUN
fnp-5883	255	189	microscopy	microscopy	NOUN
fnp-5883	255	190	high	high	ADJ
fnp-5883	255	191	quality	quality	NOUN
fnp-5883	255	192	ultrastructure	ultrastructure	NOUN
fnp-5883	255	193	without	without	ADP
fnp-5883	255	194	evidence	evidence	NOUN
fnp-5883	255	195	of	of	ADP
fnp-5883	255	196	ice	ice	NOUN
fnp-5883	255	197	crystals	crystal	NOUN
fnp-5883	255	198	found	find	VERB
fnp-5883	255	199	in	in	ADP
fnp-5883	255	200	most	most	ADJ
fnp-5883	255	201	images	image	NOUN
fnp-5883	255	202	half	half	DET
fnp-5883	255	203	human	human	ADJ
fnp-5883	255	204	brains	brain	NOUN
fnp-5883	255	205	(	(	PUNCT
fnp-5883	255	206	vonsattel	vonsattel	NOUN
fnp-5883	255	207	et	et	PROPN
fnp-5883	255	208	al	al	PROPN
fnp-5883	255	209	.	.	PROPN
fnp-5883	255	210	,	,	PUNCT
fnp-5883	255	211	1995	1995	NUM
fnp-5883	255	212	)	)	PUNCT
fnp-5883	255	213	frozen	freeze	VERB
fnp-5883	255	214	en	en	X
fnp-5883	255	215	bloc	bloc	NOUN
fnp-5883	255	216	in	in	ADP
fnp-5883	255	217	a	a	DET
fnp-5883	255	218	freezer	freezer	NOUN
fnp-5883	255	219	or	or	CCONJ
fnp-5883	255	220	on	on	ADP
fnp-5883	255	221	dry	dry	ADJ
fnp-5883	255	222	ice	ice	NOUN
fnp-5883	255	223	entire	entire	ADJ
fnp-5883	255	224	specimen	speciman	NOUN
fnp-5883	255	225	warmed	warm	VERB
fnp-5883	255	226	,	,	PUNCT
fnp-5883	255	227	cut	cut	VERB
fnp-5883	255	228	prior	prior	ADV
fnp-5883	255	229	to	to	ADP
fnp-5883	255	230	microscopy	microscopy	NOUN
fnp-5883	255	231	severe	severe	ADJ
fnp-5883	255	232	freezing	freezing	NOUN
fnp-5883	255	233	artifacts	artifact	NOUN
fnp-5883	255	234	so	so	SCONJ
fnp-5883	255	235	that	that	SCONJ
fnp-5883	255	236	it	it	PRON
fnp-5883	255	237	is	be	AUX
fnp-5883	255	238	difficult	difficult	ADJ
fnp-5883	255	239	for	for	SCONJ
fnp-5883	255	240	experienced	experienced	ADJ
fnp-5883	255	241	people	people	NOUN
fnp-5883	255	242	to	to	PART
fnp-5883	255	243	distinguish	distinguish	VERB
fnp-5883	255	244	cells	cell	NOUN
fnp-5883	255	245	10	10	NUM
fnp-5883	255	246	to	to	PART
fnp-5883	255	247	12	12	NUM
fnp-5883	255	248	 	 	SPACE
fnp-5883	255	249	mm	mm	PROPN
fnp-5883	255	250	thick	thick	ADJ
fnp-5883	255	251	slices	slice	NOUN
fnp-5883	255	252	of	of	ADP
fnp-5883	255	253	human	human	ADJ
fnp-5883	255	254	brain	brain	NOUN
fnp-5883	255	255	(	(	PUNCT
fnp-5883	255	256	meyronet	meyronet	NOUN
fnp-5883	255	257	et	et	PROPN
fnp-5883	255	258	al	al	PROPN
fnp-5883	255	259	.	.	PROPN
fnp-5883	255	260	,	,	PUNCT
fnp-5883	255	261	2015	2015	NUM
fnp-5883	255	262	)	)	PUNCT
fnp-5883	255	263	surrounded	surround	VERB
fnp-5883	255	264	by	by	ADP
fnp-5883	255	265	large	large	ADJ
fnp-5883	255	266	pulverized	pulverize	VERB
fnp-5883	255	267	dry	dry	ADJ
fnp-5883	255	268	ice	ice	NOUN
fnp-5883	255	269	,	,	PUNCT
fnp-5883	255	270	at	at	ADP
fnp-5883	255	271	least	least	ADJ
fnp-5883	255	272	2x	2x	NUM
fnp-5883	255	273	the	the	DET
fnp-5883	255	274	volume	volume	NOUN
fnp-5883	255	275	of	of	ADP
fnp-5883	255	276	the	the	DET
fnp-5883	255	277	tissue	tissue	NOUN
fnp-5883	255	278	cryostat	cryostat	NOUN
fnp-5883	255	279	sections	section	NOUN
fnp-5883	255	280	,	,	PUNCT
fnp-5883	255	281	light	light	NOUN
fnp-5883	255	282	microscopy	microscopy	VERB
fnp-5883	255	283	high	high	ADJ
fnp-5883	255	284	quality	quality	NOUN
fnp-5883	255	285	preservation	preservation	NOUN
fnp-5883	255	286	of	of	ADP
fnp-5883	255	287	brain	brain	NOUN
fnp-5883	255	288	tissue	tissue	NOUN
fnp-5883	255	289	architecture	architecture	NOUN
fnp-5883	255	290	and	and	CCONJ
fnp-5883	255	291	cellular	cellular	ADJ
fnp-5883	255	292	morphology	morphology	NOUN
fnp-5883	255	293	surgical	surgical	ADJ
fnp-5883	255	294	biopsies	biopsy	NOUN
fnp-5883	255	295	of	of	ADP
fnp-5883	255	296	neoplastic	neoplastic	ADJ
fnp-5883	255	297	brain	brain	NOUN
fnp-5883	255	298	tissue	tissue	NOUN
fnp-5883	255	299	(	(	PUNCT
fnp-5883	255	300	priemer	priemer	NOUN
fnp-5883	255	301	et	et	PROPN
fnp-5883	255	302	al	al	PROPN
fnp-5883	255	303	.	.	PROPN
fnp-5883	255	304	,	,	PUNCT
fnp-5883	255	305	2023	2023	NUM
fnp-5883	255	306	)	)	PUNCT
fnp-5883	255	307	covered	cover	VERB
fnp-5883	255	308	in	in	ADP
fnp-5883	255	309	oct	oct	PROPN
fnp-5883	255	310	compound	compound	NOUN
fnp-5883	255	311	and	and	CCONJ
fnp-5883	255	312	frozen	freeze	VERB
fnp-5883	255	313	in	in	ADP
fnp-5883	255	314	a	a	DET
fnp-5883	255	315	cryostat	cryostat	NOUN
fnp-5883	255	316	at	at	ADP
fnp-5883	255	317	‑18	‑18	NOUN
fnp-5883	255	318	to	to	ADP
fnp-5883	255	319	‑21	‑21	NOUN
fnp-5883	255	320	 	 	SPACE
fnp-5883	255	321	°	°	PROPN
fnp-5883	255	322	c	c	NOUN
fnp-5883	255	323	cryostat	cryostat	NOUN
fnp-5883	255	324	sections	section	NOUN
fnp-5883	255	325	,	,	PUNCT
fnp-5883	255	326	light	light	ADJ
fnp-5883	255	327	microscopy	microscopy	NOUN
fnp-5883	255	328	,	,	PUNCT
fnp-5883	255	329	h&e	h&e	PROPN
fnp-5883	255	330	staining	stain	VERB
fnp-5883	255	331	high	high	ADJ
fnp-5883	255	332	ice	ice	NOUN
fnp-5883	255	333	crystal	crystal	NOUN
fnp-5883	255	334	and	and	CCONJ
fnp-5883	255	335	vacuole	vacuole	NOUN
fnp-5883	255	336	formation	formation	NOUN
fnp-5883	255	337	,	,	PUNCT
fnp-5883	255	338	lower	low	ADJ
fnp-5883	255	339	preservation	preservation	NOUN
fnp-5883	255	340	quality	quality	NOUN
fnp-5883	255	341	than	than	ADP
fnp-5883	255	342	faster	fast	ADV
fnp-5883	255	343	cooled	cool	VERB
fnp-5883	255	344	samples	sample	NOUN
fnp-5883	255	345	in	in	ADP
fnp-5883	255	346	the	the	DET
fnp-5883	255	347	same	same	ADJ
fnp-5883	255	348	study	study	NOUN
fnp-5883	255	349	table	table	NOUN
fnp-5883	255	350	 	 	SPACE
fnp-5883	255	351	3	3	NUM
fnp-5883	255	352	.	.	PUNCT
fnp-5883	255	353	reported	report	VERB
fnp-5883	255	354	histologic	histologic	ADJ
fnp-5883	255	355	effects	effect	NOUN
fnp-5883	255	356	in	in	ADP
fnp-5883	255	357	representative	representative	ADJ
fnp-5883	255	358	observations	observation	NOUN
fnp-5883	255	359	from	from	ADP
fnp-5883	255	360	freezing	freeze	VERB
fnp-5883	255	361	studies	study	NOUN
fnp-5883	255	362	.	.	PUNCT
fnp-5883	256	1	h&e	h&e	PROPN
fnp-5883	256	2	:	:	PUNCT
fnp-5883	256	3	hematoxylin	hematoxylin	PROPN
fnp-5883	256	4	and	and	CCONJ
fnp-5883	256	5	eosin	eosin	PROPN
fnp-5883	256	6	,	,	PUNCT
fnp-5883	256	7	oct	oct	NOUN
fnp-5883	256	8	:	:	PUNCT
fnp-5883	256	9	optimal	optimal	ADJ
fnp-5883	256	10	cutting	cut	VERB
fnp-5883	256	11	temperature	temperature	NOUN
fnp-5883	256	12	.	.	PUNCT
fnp-5883	257	1	comparison	comparison	NOUN
fnp-5883	257	2	of	of	ADP
fnp-5883	257	3	methods	method	NOUN
fnp-5883	257	4	the	the	DET
fnp-5883	257	5	differences	difference	NOUN
fnp-5883	257	6	in	in	ADP
fnp-5883	257	7	histologic	histologic	ADJ
fnp-5883	257	8	outcomes	outcome	NOUN
fnp-5883	257	9	between	between	ADP
fnp-5883	257	10	these	these	DET
fnp-5883	257	11	freezing	freezing	ADJ
fnp-5883	257	12	methods	method	NOUN
fnp-5883	257	13	is	be	AUX
fnp-5883	257	14	thought	think	VERB
fnp-5883	257	15	to	to	PART
fnp-5883	257	16	largely	largely	ADV
fnp-5883	257	17	stem	stem	VERB
fnp-5883	257	18	from	from	ADP
fnp-5883	257	19	their	their	PRON
fnp-5883	257	20	different	different	ADJ
fnp-5883	257	21	cooling	cool	VERB
fnp-5883	257	22	rates	rate	NOUN
fnp-5883	257	23	,	,	PUNCT
fnp-5883	257	24	which	which	PRON
fnp-5883	257	25	in	in	ADP
fnp-5883	257	26	turn	turn	NOUN
fnp-5883	257	27	is	be	AUX
fnp-5883	257	28	related	relate	VERB
fnp-5883	257	29	to	to	ADP
fnp-5883	257	30	the	the	DET
fnp-5883	257	31	properties	property	NOUN
fnp-5883	257	32	of	of	ADP
fnp-5883	257	33	the	the	DET
fnp-5883	257	34	cooling	cool	VERB
fnp-5883	257	35	media	medium	NOUN
fnp-5883	257	36	.	.	PUNCT
fnp-5883	258	1	freezer	freezer	NOUN
fnp-5883	258	2	-	-	PUNCT
fnp-5883	258	3	based	base	VERB
fnp-5883	258	4	methods	method	NOUN
fnp-5883	258	5	,	,	PUNCT
fnp-5883	258	6	while	while	SCONJ
fnp-5883	258	7	practical	practical	ADJ
fnp-5883	258	8	for	for	ADP
fnp-5883	258	9	long	long	ADJ
fnp-5883	258	10	-	-	PUNCT
fnp-5883	258	11	term	term	NOUN
fnp-5883	258	12	storage	storage	NOUN
fnp-5883	258	13	,	,	PUNCT
fnp-5883	258	14	typically	typically	ADV
fnp-5883	258	15	offer	offer	VERB
fnp-5883	258	16	slow	slow	ADJ
fnp-5883	258	17	cooling	cool	VERB
fnp-5883	258	18	rates	rate	NOUN
fnp-5883	258	19	because	because	SCONJ
fnp-5883	258	20	they	they	PRON
fnp-5883	258	21	rely	rely	VERB
fnp-5883	258	22	on	on	ADP
fnp-5883	258	23	convective	convective	ADJ
fnp-5883	258	24	heat	heat	NOUN
fnp-5883	258	25	transfer	transfer	NOUN
fnp-5883	258	26	via	via	ADP
fnp-5883	258	27	the	the	DET
fnp-5883	258	28	air	air	NOUN
fnp-5883	258	29	,	,	PUNCT
fnp-5883	258	30	which	which	PRON
fnp-5883	258	31	is	be	AUX
fnp-5883	258	32	a	a	DET
fnp-5883	258	33	poor	poor	ADJ
fnp-5883	258	34	thermal	thermal	ADJ
fnp-5883	258	35	conductor	conductor	NOUN
fnp-5883	258	36	.	.	PUNCT
fnp-5883	259	1	as	as	ADP
fnp-5883	259	2	a	a	DET
fnp-5883	259	3	result	result	NOUN
fnp-5883	259	4	,	,	PUNCT
fnp-5883	259	5	the	the	DET
fnp-5883	259	6	cooling	cool	VERB
fnp-5883	259	7	process	process	NOUN
fnp-5883	259	8	is	be	AUX
fnp-5883	259	9	gradual	gradual	ADJ
fnp-5883	259	10	and	and	CCONJ
fnp-5883	259	11	less	less	ADV
fnp-5883	259	12	efficient	efficient	ADJ
fnp-5883	259	13	.	.	PUNCT
fnp-5883	260	1	immersion	immersion	NOUN
fnp-5883	260	2	of	of	ADP
fnp-5883	260	3	brain	brain	NOUN
fnp-5883	260	4	slices	slice	NOUN
fnp-5883	260	5	in	in	ADP
fnp-5883	260	6	liquid	liquid	ADJ
fnp-5883	260	7	nitrogen	nitrogen	NOUN
fnp-5883	260	8	vapor	vapor	NOUN
fnp-5883	260	9	,	,	PUNCT
fnp-5883	260	10	although	although	SCONJ
fnp-5883	260	11	also	also	ADV
fnp-5883	260	12	a	a	DET
fnp-5883	260	13	gas	gas	NOUN
fnp-5883	260	14	,	,	PUNCT
fnp-5883	260	15	has	have	AUX
fnp-5883	260	16	been	be	AUX
fnp-5883	260	17	reported	report	VERB
fnp-5883	260	18	to	to	PART
fnp-5883	260	19	result	result	VERB
fnp-5883	260	20	in	in	ADP
fnp-5883	260	21	freezing	freeze	VERB
fnp-5883	260	22	with	with	ADP
fnp-5883	260	23	good	good	ADJ
fnp-5883	260	24	morphological	morphological	ADJ
fnp-5883	260	25	preservation	preservation	NOUN
fnp-5883	260	26	(	(	PUNCT
fnp-5883	260	27	vonsattel	vonsattel	NOUN
fnp-5883	260	28	et	et	PROPN
fnp-5883	260	29	al	al	PROPN
fnp-5883	260	30	.	.	PROPN
fnp-5883	260	31	,	,	PUNCT
fnp-5883	260	32	1995	1995	NUM
fnp-5883	260	33	;	;	PUNCT
fnp-5883	260	34	davis	davis	PROPN
fnp-5883	260	35	et	et	PROPN
fnp-5883	260	36	al	al	PROPN
fnp-5883	260	37	.	.	PROPN
fnp-5883	260	38	,	,	PUNCT
fnp-5883	260	39	2019	2019	NUM
fnp-5883	260	40	)	)	PUNCT
fnp-5883	260	41	.	.	PUNCT
fnp-5883	261	1	this	this	PRON
fnp-5883	261	2	is	be	AUX
fnp-5883	261	3	likely	likely	ADJ
fnp-5883	261	4	in	in	ADP
fnp-5883	261	5	part	part	NOUN
fnp-5883	261	6	because	because	SCONJ
fnp-5883	261	7	liquid	liquid	ADJ
fnp-5883	261	8	nitrogen	nitrogen	NOUN
fnp-5883	261	9	vapor	vapor	NOUN
fnp-5883	261	10	is	be	AUX
fnp-5883	261	11	much	much	ADV
fnp-5883	261	12	colder	cold	ADJ
fnp-5883	261	13	(	(	PUNCT
fnp-5883	261	14	often	often	ADV
fnp-5883	261	15	between	between	ADP
fnp-5883	261	16	‑150	‑150	NOUN
fnp-5883	261	17	 	 	SPACE
fnp-5883	261	18	°	°	ADP
fnp-5883	261	19	c	c	NOUN
fnp-5883	261	20	and	and	CCONJ
fnp-5883	261	21	‑196	‑196	PROPN
fnp-5883	261	22	 	 	SPACE
fnp-5883	261	23	°	°	ADP
fnp-5883	261	24	c	c	NOUN
fnp-5883	261	25	)	)	PUNCT
fnp-5883	261	26	than	than	ADP
fnp-5883	261	27	a	a	DET
fnp-5883	261	28	typical	typical	ADJ
fnp-5883	261	29	ultracold	ultracold	ADJ
fnp-5883	261	30	freezer	freezer	NOUN
fnp-5883	261	31	(	(	PUNCT
fnp-5883	261	32	‑80	‑80	NOUN
fnp-5883	261	33	 	 	SPACE
fnp-5883	261	34	°	°	ADP
fnp-5883	261	35	c	c	NOUN
fnp-5883	261	36	)	)	PUNCT
fnp-5883	261	37	.	.	PUNCT
fnp-5883	262	1	the	the	DET
fnp-5883	262	2	greater	great	ADJ
fnp-5883	262	3	temperature	temperature	NOUN
fnp-5883	262	4	differential	differential	NOUN
fnp-5883	262	5	between	between	ADP
fnp-5883	262	6	the	the	DET
fnp-5883	262	7	liquid	liquid	ADJ
fnp-5883	262	8	nitrogen	nitrogen	NOUN
fnp-5883	262	9	vapor	vapor	NOUN
fnp-5883	262	10	and	and	CCONJ
fnp-5883	262	11	the	the	DET
fnp-5883	262	12	tissue	tissue	NOUN
fnp-5883	262	13	allows	allow	VERB
fnp-5883	262	14	for	for	ADP
fnp-5883	262	15	more	more	ADV
fnp-5883	262	16	rapid	rapid	ADJ
fnp-5883	262	17	heat	heat	NOUN
fnp-5883	262	18	transfer	transfer	NOUN
fnp-5883	262	19	and	and	CCONJ
fnp-5883	262	20	cooling	cooling	NOUN
fnp-5883	262	21	,	,	PUNCT
fnp-5883	262	22	even	even	ADV
fnp-5883	262	23	though	though	SCONJ
fnp-5883	262	24	nitrogen	nitrogen	NOUN
fnp-5883	262	25	vapor	vapor	NOUN
fnp-5883	262	26	is	be	AUX
fnp-5883	262	27	still	still	ADV
fnp-5883	262	28	a	a	DET
fnp-5883	262	29	relatively	relatively	ADV
fnp-5883	262	30	poor	poor	ADJ
fnp-5883	262	31	thermal	thermal	ADJ
fnp-5883	262	32	conductor	conductor	NOUN
fnp-5883	262	33	compared	compare	VERB
fnp-5883	262	34	to	to	ADP
fnp-5883	262	35	liquids	liquid	NOUN
fnp-5883	262	36	.	.	PUNCT
fnp-5883	263	1	dry	dry	ADJ
fnp-5883	263	2	ice	ice	NOUN
fnp-5883	263	3	methods	method	NOUN
fnp-5883	263	4	provide	provide	VERB
fnp-5883	263	5	faster	fast	ADV
fnp-5883	263	6	cooling	cooling	NOUN
fnp-5883	263	7	than	than	ADP
fnp-5883	263	8	the	the	DET
fnp-5883	263	9	use	use	NOUN
fnp-5883	263	10	of	of	ADP
fnp-5883	263	11	freezers	freezer	NOUN
fnp-5883	263	12	,	,	PUNCT
fnp-5883	263	13	but	but	CCONJ
fnp-5883	263	14	the	the	DET
fnp-5883	263	15	cooling	cool	VERB
fnp-5883	263	16	rate	rate	NOUN
fnp-5883	263	17	can	can	AUX
fnp-5883	263	18	be	be	AUX
fnp-5883	263	19	inconsistent	inconsistent	ADJ
fnp-5883	263	20	depending	depend	VERB
fnp-5883	263	21	on	on	ADP
fnp-5883	263	22	how	how	SCONJ
fnp-5883	263	23	well	well	ADV
fnp-5883	263	24	the	the	DET
fnp-5883	263	25	tissue	tissue	NOUN
fnp-5883	263	26	contacts	contact	VERB
fnp-5883	263	27	the	the	DET
fnp-5883	263	28	dry	dry	ADJ
fnp-5883	263	29	ice	ice	NOUN
fnp-5883	263	30	.	.	PUNCT
fnp-5883	264	1	otherwise	otherwise	ADV
fnp-5883	264	2	,	,	PUNCT
fnp-5883	264	3	these	these	DET
fnp-5883	264	4	methods	method	NOUN
fnp-5883	264	5	face	face	VERB
fnp-5883	264	6	the	the	DET
fnp-5883	264	7	same	same	ADJ
fnp-5883	264	8	issue	issue	NOUN
fnp-5883	264	9	of	of	ADP
fnp-5883	264	10	inefficient	inefficient	ADJ
fnp-5883	264	11	heat	heat	NOUN
fnp-5883	264	12	transfer	transfer	NOUN
fnp-5883	264	13	through	through	ADP
fnp-5883	264	14	gas	gas	NOUN
fnp-5883	264	15	.	.	PUNCT
fnp-5883	265	1	freezing	freeze	VERB
fnp-5883	265	2	on	on	ADP
fnp-5883	265	3	pre	pre	ADJ
fnp-5883	265	4	-	-	ADJ
fnp-5883	265	5	cooled	cooled	ADJ
fnp-5883	265	6	metal	metal	NOUN
fnp-5883	265	7	surfaces	surface	NOUN
fnp-5883	265	8	can	can	AUX
fnp-5883	265	9	achieve	achieve	VERB
fnp-5883	265	10	very	very	ADV
fnp-5883	265	11	high	high	ADJ
fnp-5883	265	12	cooling	cool	VERB
fnp-5883	265	13	rates	rate	NOUN
fnp-5883	265	14	at	at	ADP
fnp-5883	265	15	and	and	CCONJ
fnp-5883	265	16	close	close	ADJ
fnp-5883	265	17	to	to	ADP
fnp-5883	265	18	the	the	DET
fnp-5883	265	19	freezing	freezing	ADJ
fnp-5883	265	20	surface	surface	NOUN
fnp-5883	265	21	.	.	PUNCT
fnp-5883	266	1	one	one	NUM
fnp-5883	266	2	study	study	NOUN
fnp-5883	266	3	measured	measure	VERB
fnp-5883	266	4	the	the	DET
fnp-5883	266	5	efficacy	efficacy	NOUN
fnp-5883	266	6	of	of	ADP
fnp-5883	266	7	aluminum	aluminum	NOUN
fnp-5883	266	8	sheets	sheet	NOUN
fnp-5883	266	9	pre	pre	VERB
fnp-5883	266	10	-	-	VERB
fnp-5883	266	11	cooled	cooled	ADJ
fnp-5883	266	12	with	with	ADP
fnp-5883	266	13	dry	dry	ADJ
fnp-5883	266	14	ice	ice	NOUN
fnp-5883	266	15	in	in	ADP
fnp-5883	266	16	cooling	cool	VERB
fnp-5883	266	17	1	1	NUM
fnp-5883	266	18	 	 	SPACE
fnp-5883	266	19	cm	cm	NOUN
fnp-5883	266	20	thick	thick	ADJ
fnp-5883	266	21	fresh	fresh	ADJ
fnp-5883	266	22	brain	brain	NOUN
fnp-5883	266	23	slices	slice	NOUN
fnp-5883	266	24	(	(	PUNCT
fnp-5883	266	25	nochlin	nochlin	PROPN
fnp-5883	266	26	et	et	PROPN
fnp-5883	266	27	al	al	PROPN
fnp-5883	266	28	.	.	PROPN
fnp-5883	266	29	,	,	PUNCT
fnp-5883	266	30	1993	1993	NUM
fnp-5883	266	31	)	)	PUNCT
fnp-5883	266	32	.	.	PUNCT
fnp-5883	267	1	using	use	VERB
fnp-5883	267	2	a	a	DET
fnp-5883	267	3	thermocouple	thermocouple	ADJ
fnp-5883	267	4	probe	probe	NOUN
fnp-5883	267	5	,	,	PUNCT
fnp-5883	267	6	the	the	DET
fnp-5883	267	7	authors	author	NOUN
fnp-5883	267	8	found	find	VERB
fnp-5883	267	9	that	that	SCONJ
fnp-5883	267	10	a	a	DET
fnp-5883	267	11	core	core	NOUN
fnp-5883	267	12	temperature	temperature	NOUN
fnp-5883	267	13	in	in	ADP
fnp-5883	267	14	the	the	DET
fnp-5883	267	15	slices	slice	NOUN
fnp-5883	267	16	of	of	ADP
fnp-5883	267	17	‑70	‑70	PROPN
fnp-5883	267	18	 	 	SPACE
fnp-5883	267	19	°	°	PROPN
fnp-5883	267	20	c	c	PROPN
fnp-5883	267	21	was	be	AUX
fnp-5883	267	22	reached	reach	VERB
fnp-5883	267	23	within	within	ADP
fnp-5883	267	24	10	10	NUM
fnp-5883	267	25	minutes	minute	NOUN
fnp-5883	267	26	.	.	PUNCT
fnp-5883	268	1	they	they	PRON
fnp-5883	268	2	found	find	VERB
fnp-5883	268	3	no	no	DET
fnp-5883	268	4	ice	ice	NOUN
fnp-5883	268	5	crystals	crystal	NOUN
fnp-5883	268	6	in	in	ADP
fnp-5883	268	7	most	most	ADJ
fnp-5883	268	8	light	light	ADJ
fnp-5883	268	9	micrographs	micrograph	NOUN
fnp-5883	268	10	.	.	PUNCT
fnp-5883	269	1	this	this	DET
fnp-5883	269	2	result	result	NOUN
fnp-5883	269	3	suggests	suggest	VERB
fnp-5883	269	4	that	that	SCONJ
fnp-5883	269	5	a	a	DET
fnp-5883	269	6	sufficiently	sufficiently	ADV
fnp-5883	269	7	fast	fast	ADJ
fnp-5883	269	8	cooling	cool	VERB
fnp-5883	269	9	rate	rate	NOUN
fnp-5883	269	10	was	be	AUX
fnp-5883	269	11	achieved	achieve	VERB
fnp-5883	269	12	,	,	PUNCT
fnp-5883	269	13	so	so	SCONJ
fnp-5883	269	14	that	that	SCONJ
fnp-5883	269	15	only	only	ADV
fnp-5883	269	16	relatively	relatively	ADV
fnp-5883	269	17	small	small	ADJ
fnp-5883	269	18	ice	ice	NOUN
fnp-5883	269	19	crystals	crystal	NOUN
fnp-5883	269	20	were	be	AUX
fnp-5883	269	21	formed	form	VERB
fnp-5883	269	22	.	.	PUNCT
fnp-5883	270	1	however	however	ADV
fnp-5883	270	2	,	,	PUNCT
fnp-5883	270	3	the	the	DET
fnp-5883	270	4	rate	rate	NOUN
fnp-5883	270	5	of	of	ADP
fnp-5883	270	6	freezing	freezing	NOUN
fnp-5883	270	7	when	when	SCONJ
fnp-5883	270	8	using	use	VERB
fnp-5883	270	9	cooled	cool	VERB
fnp-5883	270	10	surfaces	surface	NOUN
fnp-5883	270	11	is	be	AUX
fnp-5883	270	12	expected	expect	VERB
fnp-5883	270	13	to	to	PART
fnp-5883	270	14	decrease	decrease	VERB
fnp-5883	270	15	with	with	ADP
fnp-5883	270	16	depth	depth	NOUN
fnp-5883	270	17	.	.	PUNCT
fnp-5883	271	1	as	as	ADP
fnp-5883	271	2	a	a	DET
fnp-5883	271	3	result	result	NOUN
fnp-5883	271	4	,	,	PUNCT
fnp-5883	271	5	methods	method	NOUN
fnp-5883	271	6	relying	rely	VERB
fnp-5883	271	7	on	on	ADP
fnp-5883	271	8	physical	physical	ADJ
fnp-5883	271	9	contact	contact	NOUN
fnp-5883	271	10	with	with	ADP
fnp-5883	271	11	cooled	cool	VERB
fnp-5883	271	12	surfaces	surface	NOUN
fnp-5883	271	13	are	be	AUX
fnp-5883	271	14	more	more	ADV
fnp-5883	271	15	effective	effective	ADJ
fnp-5883	271	16	for	for	ADP
fnp-5883	271	17	preserving	preserve	VERB
fnp-5883	271	18	the	the	DET
fnp-5883	271	19	morphology	morphology	NOUN
fnp-5883	271	20	of	of	ADP
fnp-5883	271	21	surface	surface	NOUN
fnp-5883	271	22	structures	structure	NOUN
fnp-5883	271	23	,	,	PUNCT
fnp-5883	271	24	such	such	ADJ
fnp-5883	271	25	as	as	ADP
fnp-5883	271	26	brain	brain	NOUN
fnp-5883	271	27	slices	slice	NOUN
fnp-5883	271	28	,	,	PUNCT
fnp-5883	271	29	but	but	CCONJ
fnp-5883	271	30	will	will	AUX
fnp-5883	271	31	be	be	AUX
fnp-5883	271	32	less	less	ADV
fnp-5883	271	33	effective	effective	ADJ
fnp-5883	271	34	for	for	ADP
fnp-5883	271	35	larger	large	ADJ
fnp-5883	271	36	specimens	specimen	NOUN
fnp-5883	271	37	,	,	PUNCT
fnp-5883	271	38	especially	especially	ADV
fnp-5883	271	39	if	if	SCONJ
fnp-5883	271	40	the	the	DET
fnp-5883	271	41	tissue	tissue	NOUN
fnp-5883	271	42	is	be	AUX
fnp-5883	271	43	not	not	PART
fnp-5883	271	44	flat	flat	ADJ
fnp-5883	271	45	.	.	PUNCT
fnp-5883	272	1	when	when	SCONJ
fnp-5883	272	2	comparing	compare	VERB
fnp-5883	272	3	isopentane	isopentane	NOUN
fnp-5883	272	4	and	and	CCONJ
fnp-5883	272	5	liquid	liquid	ADJ
fnp-5883	272	6	nitrogen	nitrogen	NOUN
fnp-5883	272	7	immersion	immersion	NOUN
fnp-5883	272	8	,	,	PUNCT
fnp-5883	272	9	isopentane	isopentane	NOUN
fnp-5883	272	10	is	be	AUX
fnp-5883	272	11	generally	generally	ADV
fnp-5883	272	12	preferred	preferred	ADJ
fnp-5883	272	13	(	(	PUNCT
fnp-5883	272	14	wieser	wieser	PROPN
fnp-5883	272	15	et	et	PROPN
fnp-5883	272	16	al	al	PROPN
fnp-5883	272	17	.	.	PROPN
fnp-5883	272	18	,	,	PUNCT
fnp-5883	272	19	2022	2022	NUM
fnp-5883	272	20	)	)	PUNCT
fnp-5883	272	21	.	.	PUNCT
fnp-5883	273	1	the	the	DET
fnp-5883	273	2	key	key	ADJ
fnp-5883	273	3	reason	reason	NOUN
fnp-5883	273	4	is	be	AUX
fnp-5883	273	5	that	that	SCONJ
fnp-5883	273	6	liquid	liquid	ADJ
fnp-5883	273	7	nitrogen	nitrogen	NOUN
fnp-5883	273	8	has	have	VERB
fnp-5883	273	9	an	an	DET
fnp-5883	273	10	extremely	extremely	ADV
fnp-5883	273	11	low	low	ADJ
fnp-5883	273	12	boiling	boiling	NOUN
fnp-5883	273	13	point	point	NOUN
fnp-5883	273	14	of	of	ADP
fnp-5883	273	15	‑196	‑196	PROPN
fnp-5883	273	16	 	 	SPACE
fnp-5883	273	17	°	°	ADP
fnp-5883	273	18	c	c	PROPN
fnp-5883	273	19	.	.	PUNCT
fnp-5883	274	1	this	this	PRON
fnp-5883	274	2	means	mean	VERB
fnp-5883	274	3	that	that	SCONJ
fnp-5883	274	4	even	even	ADV
fnp-5883	274	5	a	a	DET
fnp-5883	274	6	slight	slight	ADJ
fnp-5883	274	7	degree	degree	NOUN
fnp-5883	274	8	of	of	ADP
fnp-5883	274	9	warming	warming	NOUN
fnp-5883	274	10	due	due	ADP
fnp-5883	274	11	to	to	ADP
fnp-5883	274	12	the	the	DET
fnp-5883	274	13	specimen	speciman	NOUN
fnp-5883	274	14	being	be	AUX
fnp-5883	274	15	plunged	plunge	VERB
fnp-5883	274	16	into	into	ADP
fnp-5883	274	17	it	it	PRON
fnp-5883	274	18	causes	cause	VERB
fnp-5883	274	19	a	a	DET
fnp-5883	274	20	rapid	rapid	ADJ
fnp-5883	274	21	vaporization	vaporization	NOUN
fnp-5883	274	22	that	that	PRON
fnp-5883	274	23	leads	lead	VERB
fnp-5883	274	24	to	to	ADP
fnp-5883	274	25	an	an	DET
fnp-5883	274	26	insulating	insulate	VERB
fnp-5883	274	27	layer	layer	NOUN
fnp-5883	274	28	of	of	ADP
fnp-5883	274	29	vapor	vapor	NOUN
fnp-5883	274	30	around	around	ADP
fnp-5883	274	31	the	the	DET
fnp-5883	274	32	specimen	speciman	NOUN
fnp-5883	274	33	that	that	PRON
fnp-5883	274	34	impedes	impede	VERB
fnp-5883	274	35	effective	effective	ADJ
fnp-5883	274	36	heat	heat	NOUN
fnp-5883	274	37	transfer	transfer	NOUN
fnp-5883	274	38	out	out	ADP
fnp-5883	274	39	of	of	ADP
fnp-5883	274	40	the	the	DET
fnp-5883	274	41	specimen	speciman	NOUN
fnp-5883	274	42	.	.	PUNCT
fnp-5883	275	1	this	this	PRON
fnp-5883	275	2	is	be	AUX
fnp-5883	275	3	known	know	VERB
fnp-5883	275	4	as	as	ADP
fnp-5883	275	5	the	the	DET
fnp-5883	275	6	leidenfrost	leidenfrost	ADJ
fnp-5883	275	7	effect	effect	NOUN
fnp-5883	275	8	.	.	PUNCT
fnp-5883	276	1	on	on	ADP
fnp-5883	276	2	the	the	DET
fnp-5883	276	3	other	other	ADJ
fnp-5883	276	4	hand	hand	NOUN
fnp-5883	276	5	,	,	PUNCT
fnp-5883	276	6	isopentane	isopentane	NOUN
fnp-5883	276	7	cooled	cool	VERB
fnp-5883	276	8	by	by	ADP
fnp-5883	276	9	a	a	DET
fnp-5883	276	10	different	different	ADJ
fnp-5883	276	11	cryogen	cryogen	NOUN
fnp-5883	276	12	remains	remain	VERB
fnp-5883	276	13	liquid	liquid	ADJ
fnp-5883	276	14	at	at	ADP
fnp-5883	276	15	temperatures	temperature	NOUN
fnp-5883	276	16	well	well	ADV
fnp-5883	276	17	below	below	ADP
fnp-5883	276	18	its	its	PRON
fnp-5883	276	19	boiling	boiling	NOUN
fnp-5883	276	20	point	point	NOUN
fnp-5883	276	21	of	of	ADP
fnp-5883	276	22	28	28	NUM
fnp-5883	276	23	 	 	SPACE
fnp-5883	276	24	°	°	NOUN
fnp-5883	276	25	c	c	NOUN
fnp-5883	276	26	.	.	PUNCT
fnp-5883	277	1	this	this	DET
fnp-5883	277	2	large	large	ADJ
fnp-5883	277	3	gap	gap	NOUN
fnp-5883	277	4	between	between	ADP
fnp-5883	277	5	its	its	PRON
fnp-5883	277	6	cooled	cooled	ADJ
fnp-5883	277	7	temperature	temperature	NOUN
fnp-5883	277	8	and	and	CCONJ
fnp-5883	277	9	boiling	boiling	NOUN
fnp-5883	277	10	point	point	NOUN
fnp-5883	277	11	allows	allow	VERB
fnp-5883	277	12	isopentane	isopentane	NOUN
fnp-5883	277	13	to	to	PART
fnp-5883	277	14	maintain	maintain	VERB
fnp-5883	277	15	direct	direct	ADJ
fnp-5883	277	16	contact	contact	NOUN
fnp-5883	277	17	with	with	ADP
fnp-5883	277	18	the	the	DET
fnp-5883	277	19	specimen	speciman	NOUN
fnp-5883	277	20	without	without	ADP
fnp-5883	277	21	forming	form	VERB
fnp-5883	277	22	a	a	DET
fnp-5883	277	23	vapor	vapor	NOUN
fnp-5883	277	24	barrier	barrier	NOUN
fnp-5883	277	25	,	,	PUNCT
fnp-5883	277	26	thus	thus	ADV
fnp-5883	277	27	enabling	enable	VERB
fnp-5883	277	28	more	more	ADV
fnp-5883	277	29	efficient	efficient	ADJ
fnp-5883	277	30	heat	heat	NOUN
fnp-5883	277	31	transfer	transfer	NOUN
fnp-5883	277	32	and	and	CCONJ
fnp-5883	277	33	rapid	rapid	ADJ
fnp-5883	277	34	cooling	cooling	NOUN
fnp-5883	277	35	.	.	PUNCT
fnp-5883	278	1	in	in	ADP
fnp-5883	278	2	other	other	ADJ
fnp-5883	278	3	words	word	NOUN
fnp-5883	278	4	,	,	PUNCT
fnp-5883	278	5	cooled	cool	VERB
fnp-5883	278	6	isopentane	isopentane	NOUN
fnp-5883	278	7	is	be	AUX
fnp-5883	278	8	not	not	PART
fnp-5883	278	9	susceptible	susceptible	ADJ
fnp-5883	278	10	to	to	ADP
fnp-5883	278	11	the	the	DET
fnp-5883	278	12	leidenfrost	leidenfrost	ADJ
fnp-5883	278	13	effect	effect	NOUN
fnp-5883	278	14	.	.	PUNCT
fnp-5883	279	1	for	for	ADP
fnp-5883	279	2	this	this	DET
fnp-5883	279	3	reason	reason	NOUN
fnp-5883	279	4	,	,	PUNCT
fnp-5883	279	5	pre	pre	ADJ
fnp-5883	279	6	-	-	ADJ
fnp-5883	279	7	cooled	cooled	ADJ
fnp-5883	279	8	isopentane	isopentane	NOUN
fnp-5883	279	9	allows	allow	VERB
fnp-5883	279	10	for	for	ADP
fnp-5883	279	11	better	well	ADJ
fnp-5883	279	12	thermal	thermal	ADJ
fnp-5883	279	13	contact	contact	NOUN
fnp-5883	279	14	as	as	ADV
fnp-5883	279	15	well	well	ADV
fnp-5883	279	16	as	as	ADP
fnp-5883	279	17	the	the	DET
fnp-5883	279	18	potential	potential	NOUN
fnp-5883	279	19	for	for	ADP
fnp-5883	279	20	very	very	ADV
fnp-5883	279	21	low	low	ADJ
fnp-5883	279	22	temperature	temperature	NOUN
fnp-5883	279	23	,	,	PUNCT
fnp-5883	279	24	making	make	VERB
fnp-5883	279	25	it	it	PRON
fnp-5883	279	26	a	a	DET
fnp-5883	279	27	commonly	commonly	ADV
fnp-5883	279	28	preferred	prefer	VERB
fnp-5883	279	29	option	option	NOUN
fnp-5883	279	30	in	in	ADP
fnp-5883	279	31	biobanking	biobanking	NOUN
fnp-5883	279	32	.	.	PUNCT
fnp-5883	280	1	one	one	NUM
fnp-5883	280	2	study	study	NOUN
fnp-5883	280	3	measured	measure	VERB
fnp-5883	280	4	the	the	DET
fnp-5883	280	5	rate	rate	NOUN
fnp-5883	280	6	of	of	ADP
fnp-5883	280	7	cooling	cool	VERB
fnp-5883	280	8	using	use	VERB
fnp-5883	280	9	dry	dry	ADJ
fnp-5883	280	10	ice	ice	NOUN
fnp-5883	280	11	and	and	CCONJ
fnp-5883	280	12	dry	dry	ADJ
fnp-5883	280	13	ice	ice	NOUN
fnp-5883	280	14	-	-	PUNCT
fnp-5883	280	15	cooled	cool	VERB
fnp-5883	280	16	isopentane	isopentane	NOUN
fnp-5883	280	17	in	in	ADP
fnp-5883	280	18	a	a	DET
fnp-5883	280	19	albumin	albumin	ADJ
fnp-5883	280	20	-	-	PUNCT
fnp-5883	280	21	gelatin	gelatin	NOUN
fnp-5883	280	22	block	block	NOUN
fnp-5883	280	23	meant	mean	VERB
fnp-5883	280	24	to	to	PART
fnp-5883	280	25	simulate	simulate	VERB
fnp-5883	280	26	brain	brain	NOUN
fnp-5883	280	27	tissue	tissue	NOUN
fnp-5883	280	28	(	(	PUNCT
fnp-5883	280	29	rosene	rosene	NOUN
fnp-5883	280	30	et	et	PROPN
fnp-5883	280	31	al	al	PROPN
fnp-5883	280	32	.	.	PROPN
fnp-5883	280	33	,	,	PUNCT
fnp-5883	280	34	1986	1986	NUM
fnp-5883	280	35	)	)	PUNCT
fnp-5883	280	36	.	.	PUNCT
fnp-5883	281	1	they	they	PRON
fnp-5883	281	2	found	find	VERB
fnp-5883	281	3	that	that	SCONJ
fnp-5883	281	4	dry	dry	ADJ
fnp-5883	281	5	ice	ice	NOUN
fnp-5883	281	6	led	lead	VERB
fnp-5883	281	7	to	to	ADP
fnp-5883	281	8	cooling	cool	VERB
fnp-5883	281	9	rates	rate	NOUN
fnp-5883	281	10	of	of	ADP
fnp-5883	281	11	‑1.5	‑1.5	NOUN
fnp-5883	281	12	to	to	ADP
fnp-5883	281	13	‑1.7	‑1.7	PROPN
fnp-5883	281	14	 	 	SPACE
fnp-5883	281	15	°	°	ADP
fnp-5883	281	16	c	c	PROPN
fnp-5883	281	17	/	/	SYM
fnp-5883	281	18	min	min	NOUN
fnp-5883	281	19	,	,	PUNCT
fnp-5883	281	20	while	while	SCONJ
fnp-5883	281	21	dry	dry	ADJ
fnp-5883	281	22	-	-	PUNCT
fnp-5883	281	23	ice	ice	NOUN
fnp-5883	281	24	cooled	cool	VERB
fnp-5883	281	25	isopentane	isopentane	NOUN
fnp-5883	281	26	led	lead	VERB
fnp-5883	281	27	to	to	ADP
fnp-5883	281	28	cooling	cool	VERB
fnp-5883	281	29	rates	rate	NOUN
fnp-5883	281	30	of	of	ADP
fnp-5883	281	31	‑3.2	‑3.2	NOUN
fnp-5883	281	32	to	to	ADP
fnp-5883	281	33	‑3.6	‑3.6	PRON
fnp-5883	281	34	 	 	SPACE
fnp-5883	281	35	°	°	PROPN
fnp-5883	281	36	c	c	NOUN
fnp-5883	281	37	/	/	SYM
fnp-5883	281	38	min	min	NOUN
fnp-5883	281	39	,	,	PUNCT
fnp-5883	281	40	consistent	consistent	ADJ
fnp-5883	281	41	with	with	ADP
fnp-5883	281	42	isopentane	isopentane	NOUN
fnp-5883	281	43	acting	act	VERB
fnp-5883	281	44	as	as	ADP
fnp-5883	281	45	a	a	DET
fnp-5883	281	46	very	very	ADV
fnp-5883	281	47	effective	effective	ADJ
fnp-5883	281	48	cryo	cryo	NOUN
fnp-5883	281	49	-	-	PUNCT
fnp-5883	281	50	conductor	conductor	NOUN
fnp-5883	281	51	.	.	PUNCT
fnp-5883	282	1	one	one	NUM
fnp-5883	282	2	reported	report	VERB
fnp-5883	282	3	downside	downside	NOUN
fnp-5883	282	4	of	of	ADP
fnp-5883	282	5	immersion	immersion	NOUN
fnp-5883	282	6	in	in	ADP
fnp-5883	282	7	both	both	CCONJ
fnp-5883	282	8	isopentane	isopentane	NOUN
fnp-5883	282	9	and	and	CCONJ
fnp-5883	282	10	liquid	liquid	ADJ
fnp-5883	282	11	nitrogen	nitrogen	NOUN
fnp-5883	282	12	is	be	AUX
fnp-5883	282	13	the	the	DET
fnp-5883	282	14	higher	high	ADJ
fnp-5883	282	15	rate	rate	NOUN
fnp-5883	282	16	of	of	ADP
fnp-5883	282	17	fractures	fracture	NOUN
fnp-5883	282	18	in	in	ADP
fnp-5883	282	19	brain	brain	NOUN
fnp-5883	282	20	tissue	tissue	NOUN
fnp-5883	282	21	,	,	PUNCT
fnp-5883	282	22	as	as	SCONJ
fnp-5883	282	23	compared	compare	VERB
fnp-5883	282	24	to	to	ADP
fnp-5883	282	25	freezing	freeze	VERB
fnp-5883	282	26	in	in	ADP
fnp-5883	282	27	liquid	liquid	ADJ
fnp-5883	282	28	nitrogen	nitrogen	NOUN
fnp-5883	282	29	vapor	vapor	NOUN
fnp-5883	282	30	(	(	PUNCT
fnp-5883	282	31	vonsattel	vonsattel	NOUN
fnp-5883	282	32	et	et	PROPN
fnp-5883	282	33	al	al	PROPN
fnp-5883	282	34	.	.	PROPN
fnp-5883	282	35	,	,	PUNCT
fnp-5883	282	36	1995	1995	NUM
fnp-5883	282	37	)	)	PUNCT
fnp-5883	282	38	.	.	PUNCT
fnp-5883	283	1	as	as	SCONJ
fnp-5883	283	2	previously	previously	ADV
fnp-5883	283	3	discussed	discuss	VERB
fnp-5883	283	4	,	,	PUNCT
fnp-5883	283	5	the	the	DET
fnp-5883	283	6	fractures	fracture	NOUN
fnp-5883	283	7	may	may	AUX
fnp-5883	283	8	be	be	AUX
fnp-5883	283	9	caused	cause	VERB
fnp-5883	283	10	by	by	ADP
fnp-5883	283	11	mechanical	mechanical	ADJ
fnp-5883	283	12	stress	stress	NOUN
fnp-5883	283	13	due	due	ADP
fnp-5883	283	14	to	to	ADP
fnp-5883	283	15	sudden	sudden	ADJ
fnp-5883	283	16	temperature	temperature	NOUN
fnp-5883	283	17	changes	change	NOUN
fnp-5883	283	18	(	(	PUNCT
fnp-5883	283	19	adam	adam	PROPN
fnp-5883	283	20	et	et	PROPN
fnp-5883	283	21	al	al	PROPN
fnp-5883	283	22	.	.	PROPN
fnp-5883	283	23	,	,	PUNCT
fnp-5883	283	24	1990	1990	NUM
fnp-5883	283	25	)	)	PUNCT
fnp-5883	283	26	.	.	PUNCT
fnp-5883	284	1	pulverized	pulverize	VERB
fnp-5883	284	2	dry	dry	ADJ
fnp-5883	284	3	ice	ice	NOUN
fnp-5883	284	4	has	have	AUX
fnp-5883	284	5	been	be	AUX
fnp-5883	284	6	reported	report	VERB
fnp-5883	284	7	to	to	PART
fnp-5883	284	8	be	be	AUX
fnp-5883	284	9	able	able	ADJ
fnp-5883	284	10	to	to	PART
fnp-5883	284	11	cryopreserve	cryopreserve	VERB
fnp-5883	284	12	brain	brain	NOUN
fnp-5883	284	13	hemispheres	hemisphere	NOUN
fnp-5883	284	14	without	without	ADP
fnp-5883	284	15	causing	cause	VERB
fnp-5883	284	16	deep	deep	ADJ
fnp-5883	284	17	crevasses	crevasse	NOUN
fnp-5883	284	18	due	due	ADJ
fnp-5883	284	19	to	to	ADP
fnp-5883	284	20	opposed	oppose	VERB
fnp-5883	284	21	temperature	temperature	NOUN
fnp-5883	284	22	fronts	front	NOUN
fnp-5883	284	23	in	in	ADP
fnp-5883	284	24	the	the	DET
fnp-5883	284	25	specimen	speciman	NOUN
fnp-5883	284	26	(	(	PUNCT
fnp-5883	284	27	annese	annese	ADJ
fnp-5883	284	28	,	,	PUNCT
fnp-5883	284	29	2004	2004	NUM
fnp-5883	284	30	)	)	PUNCT
fnp-5883	284	31	.	.	PUNCT
fnp-5883	285	1	damage	damage	NOUN
fnp-5883	285	2	during	during	ADP
fnp-5883	285	3	tissue	tissue	NOUN
fnp-5883	285	4	rewarming	rewarme	VERB
fnp-5883	285	5	the	the	DET
fnp-5883	285	6	rewarming	rewarming	NOUN
fnp-5883	285	7	phase	phase	NOUN
fnp-5883	285	8	can	can	AUX
fnp-5883	285	9	significantly	significantly	ADV
fnp-5883	285	10	impact	impact	VERB
fnp-5883	285	11	the	the	DET
fnp-5883	285	12	final	final	ADJ
fnp-5883	285	13	tissue	tissue	NOUN
fnp-5883	285	14	morphology	morphology	NOUN
fnp-5883	285	15	and	and	CCONJ
fnp-5883	285	16	is	be	AUX
fnp-5883	285	17	often	often	ADV
fnp-5883	285	18	thought	think	VERB
fnp-5883	285	19	to	to	PART
fnp-5883	285	20	cause	cause	VERB
fnp-5883	285	21	more	more	ADJ
fnp-5883	285	22	damage	damage	NOUN
fnp-5883	285	23	than	than	ADP
fnp-5883	285	24	the	the	DET
fnp-5883	285	25	initial	initial	ADJ
fnp-5883	285	26	freezing	freezing	NOUN
fnp-5883	285	27	.	.	PUNCT
fnp-5883	286	1	this	this	PRON
fnp-5883	286	2	is	be	AUX
fnp-5883	286	3	related	relate	VERB
fnp-5883	286	4	to	to	ADP
fnp-5883	286	5	the	the	DET
fnp-5883	286	6	physics	physics	NOUN
fnp-5883	286	7	of	of	ADP
fnp-5883	286	8	ice	ice	NOUN
fnp-5883	286	9	formation	formation	NOUN
fnp-5883	286	10	.	.	PUNCT
fnp-5883	287	1	at	at	ADP
fnp-5883	287	2	higher	high	ADJ
fnp-5883	287	3	subzero	subzero	NOUN
fnp-5883	287	4	temperatures	temperature	NOUN
fnp-5883	287	5	,	,	PUNCT
fnp-5883	287	6	the	the	DET
fnp-5883	287	7	probability	probability	NOUN
fnp-5883	287	8	of	of	ADP
fnp-5883	287	9	forming	form	VERB
fnp-5883	287	10	new	new	ADJ
fnp-5883	287	11	ice	ice	NOUN
fnp-5883	287	12	nuclei	nucleus	NOUN
fnp-5883	287	13	is	be	AUX
fnp-5883	287	14	relatively	relatively	ADV
fnp-5883	287	15	low	low	ADJ
fnp-5883	287	16	,	,	PUNCT
fnp-5883	287	17	while	while	SCONJ
fnp-5883	287	18	the	the	DET
fnp-5883	287	19	rate	rate	NOUN
fnp-5883	287	20	of	of	ADP
fnp-5883	287	21	ice	ice	NOUN
fnp-5883	287	22	growth	growth	NOUN
fnp-5883	287	23	is	be	AUX
fnp-5883	287	24	relatively	relatively	ADV
fnp-5883	287	25	high	high	ADJ
fnp-5883	287	26	(	(	PUNCT
fnp-5883	287	27	fahy	fahy	PROPN
fnp-5883	287	28	and	and	CCONJ
fnp-5883	287	29	wowk	wowk	NOUN
fnp-5883	287	30	,	,	PUNCT
fnp-5883	287	31	2015	2015	NUM
fnp-5883	287	32	)	)	PUNCT
fnp-5883	287	33	.	.	PUNCT
fnp-5883	288	1	on	on	ADP
fnp-5883	288	2	the	the	DET
fnp-5883	288	3	other	other	ADJ
fnp-5883	288	4	hand	hand	NOUN
fnp-5883	288	5	,	,	PUNCT
fnp-5883	288	6	at	at	ADP
fnp-5883	288	7	low	low	ADJ
fnp-5883	288	8	subzero	subzero	NOUN
fnp-5883	288	9	temperatures	temperature	NOUN
fnp-5883	288	10	that	that	PRON
fnp-5883	288	11	are	be	AUX
fnp-5883	288	12	still	still	ADV
fnp-5883	288	13	above	above	ADP
fnp-5883	288	14	the	the	DET
fnp-5883	288	15	glass	glass	NOUN
fnp-5883	288	16	transition	transition	NOUN
fnp-5883	288	17	temperature	temperature	NOUN
fnp-5883	288	18	,	,	PUNCT
fnp-5883	288	19	the	the	DET
fnp-5883	288	20	probability	probability	NOUN
fnp-5883	288	21	of	of	ADP
fnp-5883	288	22	forming	form	VERB
fnp-5883	288	23	new	new	ADJ
fnp-5883	288	24	ice	ice	NOUN
fnp-5883	288	25	nuclei	nucleus	NOUN
fnp-5883	288	26	is	be	AUX
fnp-5883	288	27	relatively	relatively	ADV
fnp-5883	288	28	high	high	ADJ
fnp-5883	288	29	,	,	PUNCT
fnp-5883	288	30	while	while	SCONJ
fnp-5883	288	31	the	the	DET
fnp-5883	288	32	rate	rate	NOUN
fnp-5883	288	33	of	of	ADP
fnp-5883	288	34	ice	ice	NOUN
fnp-5883	288	35	growth	growth	NOUN
fnp-5883	288	36	is	be	AUX
fnp-5883	288	37	relatively	relatively	ADV
fnp-5883	288	38	low	low	ADJ
fnp-5883	288	39	.	.	PUNCT
fnp-5883	289	1	as	as	ADP
fnp-5883	289	2	a	a	DET
fnp-5883	289	3	result	result	NOUN
fnp-5883	289	4	,	,	PUNCT
fnp-5883	289	5	as	as	SCONJ
fnp-5883	289	6	the	the	DET
fnp-5883	289	7	temperature	temperature	NOUN
fnp-5883	289	8	increases	increase	VERB
fnp-5883	289	9	during	during	ADP
fnp-5883	289	10	tissue	tissue	NOUN
fnp-5883	289	11	warming	warming	NOUN
fnp-5883	289	12	,	,	PUNCT
fnp-5883	289	13	smaller	small	ADJ
fnp-5883	289	14	ice	ice	NOUN
fnp-5883	289	15	nuclei	nucleus	NOUN
fnp-5883	289	16	have	have	VERB
fnp-5883	289	17	the	the	DET
fnp-5883	289	18	opportunity	opportunity	NOUN
fnp-5883	289	19	to	to	PART
fnp-5883	289	20	recrystallize	recrystallize	VERB
fnp-5883	289	21	into	into	ADP
fnp-5883	289	22	larger	large	ADJ
fnp-5883	289	23	ice	ice	NOUN
fnp-5883	289	24	crystals	crystal	NOUN
fnp-5883	289	25	(	(	PUNCT
fnp-5883	289	26	fahy	fahy	PROPN
fnp-5883	289	27	and	and	CCONJ
fnp-5883	289	28	wowk	wowk	NOUN
fnp-5883	289	29	,	,	PUNCT
fnp-5883	289	30	2015	2015	NUM
fnp-5883	289	31	)	)	PUNCT
fnp-5883	289	32	.	.	PUNCT
fnp-5883	290	1	larger	large	ADJ
fnp-5883	290	2	ice	ice	NOUN
fnp-5883	290	3	crystals	crystal	NOUN
fnp-5883	290	4	are	be	AUX
fnp-5883	290	5	expected	expect	VERB
fnp-5883	290	6	to	to	PART
fnp-5883	290	7	cause	cause	VERB
fnp-5883	290	8	morphological	morphological	ADJ
fnp-5883	290	9	structural	structural	ADJ
fnp-5883	290	10	damage	damage	NOUN
fnp-5883	290	11	.	.	PUNCT
fnp-5883	291	1	to	to	PART
fnp-5883	291	2	mitigate	mitigate	VERB
fnp-5883	291	3	ice	ice	NOUN
fnp-5883	291	4	damage	damage	NOUN
fnp-5883	291	5	during	during	ADP
fnp-5883	291	6	rewarming	rewarming	NOUN
fnp-5883	291	7	,	,	PUNCT
fnp-5883	291	8	several	several	ADJ
fnp-5883	291	9	approaches	approach	NOUN
fnp-5883	291	10	have	have	AUX
fnp-5883	291	11	been	be	AUX
fnp-5883	291	12	developed	develop	VERB
fnp-5883	291	13	.	.	PUNCT
fnp-5883	292	1	some	some	DET
fnp-5883	292	2	sources	source	NOUN
fnp-5883	292	3	have	have	AUX
fnp-5883	292	4	reported	report	VERB
fnp-5883	292	5	that	that	SCONJ
fnp-5883	292	6	more	more	ADV
fnp-5883	292	7	rapid	rapid	ADJ
fnp-5883	292	8	thawing	thawing	NOUN
fnp-5883	292	9	of	of	ADP
fnp-5883	292	10	tissue	tissue	NOUN
fnp-5883	292	11	leads	lead	VERB
fnp-5883	292	12	to	to	ADP
fnp-5883	292	13	better	well	ADJ
fnp-5883	292	14	outcomes	outcome	NOUN
fnp-5883	292	15	.	.	PUNCT
fnp-5883	293	1	for	for	ADP
fnp-5883	293	2	example	example	NOUN
fnp-5883	293	3	,	,	PUNCT
fnp-5883	293	4	one	one	NUM
fnp-5883	293	5	study	study	NOUN
fnp-5883	293	6	found	find	VERB
fnp-5883	293	7	better	well	ADJ
fnp-5883	293	8	neural	neural	ADJ
fnp-5883	293	9	transplantation	transplantation	NOUN
fnp-5883	293	10	outcomes	outcome	NOUN
fnp-5883	293	11	after	after	ADP
fnp-5883	293	12	thawing	thaw	VERB
fnp-5883	293	13	in	in	ADP
fnp-5883	293	14	a	a	DET
fnp-5883	293	15	hot	hot	ADJ
fnp-5883	293	16	water	water	NOUN
fnp-5883	293	17	bath	bath	NOUN
fnp-5883	293	18	at	at	ADP
fnp-5883	293	19	37	37	NUM
fnp-5883	293	20	 	 	SPACE
fnp-5883	293	21	°	°	ADP
fnp-5883	293	22	c	c	NOUN
fnp-5883	293	23	for	for	ADP
fnp-5883	293	24	30–40	30–40	NUM
fnp-5883	293	25	seconds	second	NOUN
fnp-5883	293	26	,	,	PUNCT
fnp-5883	293	27	compared	compare	VERB
fnp-5883	293	28	with	with	ADP
fnp-5883	293	29	thawing	thaw	VERB
fnp-5883	293	30	by	by	ADP
fnp-5883	293	31	leaving	leave	VERB
fnp-5883	293	32	the	the	DET
fnp-5883	293	33	neural	neural	ADJ
fnp-5883	293	34	tissue	tissue	NOUN
fnp-5883	293	35	at	at	ADP
fnp-5883	293	36	room	room	NOUN
fnp-5883	293	37	temperature	temperature	NOUN
fnp-5883	293	38	for	for	ADP
fnp-5883	293	39	5–7	5–7	NUM
fnp-5883	293	40	minutes	minute	NOUN
fnp-5883	293	41	(	(	PUNCT
fnp-5883	293	42	das	das	PROPN
fnp-5883	293	43	et	et	PROPN
fnp-5883	293	44	al	al	PROPN
fnp-5883	293	45	.	.	PROPN
fnp-5883	293	46	,	,	PUNCT
fnp-5883	293	47	1983	1983	NUM
fnp-5883	293	48	)	)	PUNCT
fnp-5883	293	49	.	.	PUNCT
fnp-5883	294	1	another	another	DET
fnp-5883	294	2	source	source	NOUN
fnp-5883	294	3	found	find	VERB
fnp-5883	294	4	high	high	ADJ
fnp-5883	294	5	-	-	PUNCT
fnp-5883	294	6	quality	quality	NOUN
fnp-5883	294	7	ultrastructural	ultrastructural	ADJ
fnp-5883	294	8	preservation	preservation	NOUN
fnp-5883	294	9	of	of	ADP
fnp-5883	294	10	several	several	ADJ
fnp-5883	294	11	non	non	ADJ
fnp-5883	294	12	-	-	ADJ
fnp-5883	294	13	brain	brain	ADJ
fnp-5883	294	14	tissue	tissue	NOUN
fnp-5883	294	15	types	type	NOUN
fnp-5883	294	16	when	when	SCONJ
fnp-5883	294	17	blocks	block	NOUN
fnp-5883	294	18	were	be	AUX
fnp-5883	294	19	frozen	freeze	VERB
fnp-5883	294	20	in	in	ADP
fnp-5883	294	21	liquid	liquid	ADJ
fnp-5883	294	22	nitrogen	nitrogen	NOUN
fnp-5883	294	23	,	,	PUNCT
fnp-5883	294	24	sectioned	section	VERB
fnp-5883	294	25	into	into	ADP
fnp-5883	294	26	around	around	ADP
fnp-5883	294	27	5	5	NUM
fnp-5883	294	28	 	 	SPACE
fnp-5883	294	29	mm3	mm3	NOUN
fnp-5883	294	30	blocks	block	NOUN
fnp-5883	294	31	on	on	ADP
fnp-5883	294	32	dry	dry	ADJ
fnp-5883	294	33	ice	ice	NOUN
fnp-5883	294	34	,	,	PUNCT
fnp-5883	294	35	and	and	CCONJ
fnp-5883	294	36	then	then	ADV
fnp-5883	294	37	rapidly	rapidly	ADV
fnp-5883	294	38	thawed	thaw	VERB
fnp-5883	294	39	at	at	ADP
fnp-5883	294	40	37	37	NUM
fnp-5883	294	41	 	 	SPACE
fnp-5883	294	42	°	°	NOUN
fnp-5883	294	43	c	c	NOUN
fnp-5883	294	44	in	in	ADP
fnp-5883	294	45	fixative	fixative	ADJ
fnp-5883	294	46	(	(	PUNCT
fnp-5883	294	47	galhuber	galhuber	NOUN
fnp-5883	294	48	et	et	PROPN
fnp-5883	294	49	al	al	PROPN
fnp-5883	294	50	.	.	PROPN
fnp-5883	294	51	,	,	PUNCT
fnp-5883	294	52	2021	2021	NUM
fnp-5883	294	53	)	)	PUNCT
fnp-5883	294	54	.	.	PUNCT
fnp-5883	295	1	alternatively	alternatively	ADV
fnp-5883	295	2	,	,	PUNCT
fnp-5883	295	3	one	one	NUM
fnp-5883	295	4	protocol	protocol	NOUN
fnp-5883	295	5	involved	involve	VERB
fnp-5883	295	6	transferring	transfer	VERB
fnp-5883	295	7	frozen	frozen	ADJ
fnp-5883	295	8	tissue	tissue	NOUN
fnp-5883	295	9	directly	directly	ADV
fnp-5883	295	10	into	into	ADP
fnp-5883	295	11	chilled	chilled	ADJ
fnp-5883	295	12	fixative	fixative	ADJ
fnp-5883	295	13	solutions	solution	NOUN
fnp-5883	295	14	(	(	PUNCT
fnp-5883	295	15	ramirez	ramirez	PROPN
fnp-5883	295	16	et	et	PROPN
fnp-5883	295	17	al	al	PROPN
fnp-5883	295	18	.	.	PROPN
fnp-5883	295	19	,	,	PUNCT
fnp-5883	295	20	2018	2018	NUM
fnp-5883	295	21	)	)	PUNCT
fnp-5883	295	22	.	.	PUNCT
fnp-5883	296	1	this	this	DET
fnp-5883	296	2	study	study	NOUN
fnp-5883	296	3	reported	report	VERB
fnp-5883	296	4	minimal	minimal	ADJ
fnp-5883	296	5	freezing	freezing	NOUN
fnp-5883	296	6	artifacts	artifact	NOUN
fnp-5883	296	7	and	and	CCONJ
fnp-5883	296	8	well	well	ADV
fnp-5883	296	9	-	-	PUNCT
fnp-5883	296	10	preserved	preserve	VERB
fnp-5883	296	11	cell	cell	NOUN
fnp-5883	296	12	morphology	morphology	NOUN
fnp-5883	296	13	when	when	SCONJ
fnp-5883	296	14	transferring	transfer	VERB
fnp-5883	296	15	frozen	frozen	ADJ
fnp-5883	296	16	brain	brain	NOUN
fnp-5883	296	17	tissue	tissue	NOUN
fnp-5883	296	18	to	to	ADP
fnp-5883	296	19	1	1	NUM
fnp-5883	296	20	 	 	SPACE
fnp-5883	296	21	°	°	NOUN
fnp-5883	296	22	c	c	NOUN
fnp-5883	296	23	chilled	chill	VERB
fnp-5883	296	24	formalin	formalin	NOUN
fnp-5883	296	25	for	for	ADP
fnp-5883	296	26	72	72	NUM
fnp-5883	296	27	hours	hour	NOUN
fnp-5883	296	28	before	before	ADP
fnp-5883	296	29	further	further	ADJ
fnp-5883	296	30	processing	processing	NOUN
fnp-5883	296	31	.	.	PUNCT
fnp-5883	297	1	the	the	DET
fnp-5883	297	2	approach	approach	NOUN
fnp-5883	297	3	of	of	ADP
fnp-5883	297	4	breaking	break	VERB
fnp-5883	297	5	frozen	frozen	ADJ
fnp-5883	297	6	tissue	tissue	NOUN
fnp-5883	297	7	into	into	ADP
fnp-5883	297	8	pieces	piece	NOUN
fnp-5883	297	9	and	and	CCONJ
fnp-5883	297	10	immersing	immerse	VERB
fnp-5883	297	11	it	it	PRON
fnp-5883	297	12	into	into	ADP
fnp-5883	297	13	fixative	fixative	ADJ
fnp-5883	297	14	while	while	SCONJ
fnp-5883	297	15	the	the	DET
fnp-5883	297	16	fragments	fragment	NOUN
fnp-5883	297	17	are	be	AUX
fnp-5883	297	18	still	still	ADV
fnp-5883	297	19	frozen	freeze	VERB
fnp-5883	297	20	,	,	PUNCT
fnp-5883	297	21	prior	prior	ADV
fnp-5883	297	22	to	to	ADP
fnp-5883	297	23	fixation	fixation	NOUN
fnp-5883	297	24	at	at	ADP
fnp-5883	297	25	refrigerator	refrigerator	NOUN
fnp-5883	297	26	temperature	temperature	NOUN
fnp-5883	297	27	,	,	PUNCT
fnp-5883	297	28	has	have	AUX
fnp-5883	297	29	also	also	ADV
fnp-5883	297	30	been	be	AUX
fnp-5883	297	31	reported	report	VERB
fnp-5883	297	32	to	to	PART
fnp-5883	297	33	be	be	AUX
fnp-5883	297	34	effective	effective	ADJ
fnp-5883	297	35	in	in	ADP
fnp-5883	297	36	rewarming	rewarme	VERB
fnp-5883	297	37	frozen	frozen	ADJ
fnp-5883	297	38	pancreatic	pancreatic	ADJ
fnp-5883	297	39	tissue	tissue	NOUN
fnp-5883	297	40	(	(	PUNCT
fnp-5883	297	41	fortunato	fortunato	PROPN
fnp-5883	297	42	et	et	PROPN
fnp-5883	297	43	al	al	PROPN
fnp-5883	297	44	.	.	PROPN
fnp-5883	297	45	,	,	PUNCT
fnp-5883	297	46	2016	2016	NUM
fnp-5883	297	47	)	)	PUNCT
fnp-5883	297	48	.	.	PUNCT
fnp-5883	298	1	as	as	ADP
fnp-5883	298	2	an	an	DET
fnp-5883	298	3	additional	additional	ADJ
fnp-5883	298	4	complication	complication	NOUN
fnp-5883	298	5	,	,	PUNCT
fnp-5883	298	6	rewarming	rewarme	VERB
fnp-5883	298	7	frozen	frozen	ADJ
fnp-5883	298	8	tissue	tissue	NOUN
fnp-5883	298	9	may	may	AUX
fnp-5883	298	10	actually	actually	ADV
fnp-5883	298	11	decrease	decrease	VERB
fnp-5883	298	12	the	the	DET
fnp-5883	298	13	observed	observe	VERB
fnp-5883	298	14	burden	burden	NOUN
fnp-5883	298	15	of	of	ADP
fnp-5883	298	16	histologic	histologic	ADJ
fnp-5883	298	17	alterations	alteration	NOUN
fnp-5883	298	18	in	in	ADP
fnp-5883	298	19	some	some	DET
fnp-5883	298	20	circumstances	circumstance	NOUN
fnp-5883	298	21	.	.	PUNCT
fnp-5883	299	1	for	for	ADP
fnp-5883	299	2	example	example	NOUN
fnp-5883	299	3	,	,	PUNCT
fnp-5883	299	4	one	one	NUM
fnp-5883	299	5	study	study	NOUN
fnp-5883	299	6	using	use	VERB
fnp-5883	299	7	slow	slow	ADJ
fnp-5883	299	8	freezing	freezing	NOUN
fnp-5883	299	9	of	of	ADP
fnp-5883	299	10	rat	rat	NOUN
fnp-5883	299	11	cutaneous	cutaneous	ADJ
fnp-5883	299	12	nerves	nerve	NOUN
fnp-5883	299	13	at	at	ADP
fnp-5883	299	14	‑10	‑10	NUM
fnp-5883	299	15	 	 	SPACE
fnp-5883	299	16	°	°	ADP
fnp-5883	299	17	c	c	NOUN
fnp-5883	299	18	to	to	ADP
fnp-5883	299	19	‑15	‑15	NOUN
fnp-5883	299	20	 	 	SPACE
fnp-5883	299	21	°	°	SCONJ
fnp-5883	299	22	c	c	NOUN
fnp-5883	299	23	with	with	ADP
fnp-5883	299	24	subsequent	subsequent	ADJ
fnp-5883	299	25	freeze	freeze	NOUN
fnp-5883	299	26	substitution	substitution	NOUN
fnp-5883	299	27	followed	follow	VERB
fnp-5883	299	28	by	by	ADP
fnp-5883	299	29	electron	electron	NOUN
fnp-5883	299	30	microscopy	microscopy	NOUN
fnp-5883	299	31	found	find	VERB
fnp-5883	299	32	that	that	SCONJ
fnp-5883	299	33	the	the	DET
fnp-5883	299	34	tissue	tissue	NOUN
fnp-5883	299	35	showed	show	VERB
fnp-5883	299	36	severe	severe	ADJ
fnp-5883	299	37	ice	ice	NOUN
fnp-5883	299	38	damage	damage	NOUN
fnp-5883	299	39	and	and	CCONJ
fnp-5883	299	40	ultrastructural	ultrastructural	ADJ
fnp-5883	299	41	artifacts	artifact	NOUN
fnp-5883	299	42	(	(	PUNCT
fnp-5883	299	43	menz	menz	NOUN
fnp-5883	299	44	,	,	PUNCT
fnp-5883	299	45	1971	1971	NUM
fnp-5883	299	46	)	)	PUNCT
fnp-5883	299	47	.	.	PUNCT
fnp-5883	300	1	only	only	ADV
fnp-5883	300	2	some	some	DET
fnp-5883	300	3	ultrastructural	ultrastructural	ADJ
fnp-5883	300	4	features	feature	NOUN
fnp-5883	300	5	could	could	AUX
fnp-5883	300	6	be	be	AUX
fnp-5883	300	7	recognized	recognize	VERB
fnp-5883	300	8	,	,	PUNCT
fnp-5883	300	9	which	which	PRON
fnp-5883	300	10	was	be	AUX
fnp-5883	300	11	attributed	attribute	VERB
fnp-5883	300	12	to	to	ADP
fnp-5883	300	13	severe	severe	ADJ
fnp-5883	300	14	dehydration	dehydration	NOUN
fnp-5883	300	15	of	of	ADP
fnp-5883	300	16	other	other	ADJ
fnp-5883	300	17	structures	structure	NOUN
fnp-5883	300	18	such	such	ADJ
fnp-5883	300	19	as	as	ADP
fnp-5883	300	20	neurofilaments	neurofilament	NOUN
fnp-5883	300	21	and	and	CCONJ
fnp-5883	300	22	mitochondria	mitochondria	NOUN
fnp-5883	300	23	.	.	PUNCT
fnp-5883	301	1	however	however	ADV
fnp-5883	301	2	,	,	PUNCT
fnp-5883	301	3	when	when	SCONJ
fnp-5883	301	4	the	the	DET
fnp-5883	301	5	slowly	slowly	ADV
fnp-5883	301	6	frozen	frozen	ADJ
fnp-5883	301	7	tissue	tissue	NOUN
fnp-5883	301	8	was	be	AUX
fnp-5883	301	9	rewarmed	rewarme	VERB
fnp-5883	301	10	via	via	ADP
fnp-5883	301	11	rapid	rapid	ADJ
fnp-5883	301	12	thawing	thawing	NOUN
fnp-5883	301	13	to	to	ADP
fnp-5883	301	14	40	40	NUM
fnp-5883	301	15	 	 	SPACE
fnp-5883	301	16	°	°	NOUN
fnp-5883	301	17	c	c	NOUN
fnp-5883	301	18	prior	prior	ADV
fnp-5883	301	19	to	to	ADP
fnp-5883	301	20	fixation	fixation	NOUN
fnp-5883	301	21	,	,	PUNCT
fnp-5883	301	22	electron	electron	NOUN
fnp-5883	301	23	microscopy	microscopy	NOUN
fnp-5883	301	24	showed	show	VERB
fnp-5883	301	25	an	an	DET
fnp-5883	301	26	improved	improved	ADJ
fnp-5883	301	27	preservation	preservation	NOUN
fnp-5883	301	28	of	of	ADP
fnp-5883	301	29	neurofilaments	neurofilament	NOUN
fnp-5883	301	30	and	and	CCONJ
fnp-5883	301	31	mitochondria	mitochondria	PROPN
fnp-5883	301	32	.	.	PUNCT
fnp-5883	302	1	notably	notably	ADV
fnp-5883	302	2	,	,	PUNCT
fnp-5883	302	3	there	there	PRON
fnp-5883	302	4	were	be	VERB
fnp-5883	302	5	still	still	ADV
fnp-5883	302	6	numerous	numerous	ADJ
fnp-5883	302	7	artifacts	artifact	NOUN
fnp-5883	302	8	;	;	PUNCT
fnp-5883	302	9	for	for	ADP
fnp-5883	302	10	example	example	NOUN
fnp-5883	302	11	,	,	PUNCT
fnp-5883	302	12	the	the	DET
fnp-5883	302	13	myelin	myelin	NOUN
fnp-5883	302	14	layers	layer	NOUN
fnp-5883	302	15	were	be	AUX
fnp-5883	302	16	disturbed	disturb	VERB
fnp-5883	302	17	,	,	PUNCT
fnp-5883	302	18	and	and	CCONJ
fnp-5883	302	19	the	the	DET
fnp-5883	302	20	mitochondria	mitochondria	NOUN
fnp-5883	302	21	were	be	AUX
fnp-5883	302	22	empty	empty	ADJ
fnp-5883	302	23	and	and	CCONJ
fnp-5883	302	24	lacked	lack	VERB
fnp-5883	302	25	clearly	clearly	ADV
fnp-5883	302	26	defined	define	VERB
fnp-5883	302	27	membranes	membrane	NOUN
fnp-5883	302	28	.	.	PUNCT
fnp-5883	303	1	mechanistically	mechanistically	ADV
fnp-5883	303	2	,	,	PUNCT
fnp-5883	303	3	the	the	DET
fnp-5883	303	4	dehydration	dehydration	NOUN
fnp-5883	303	5	that	that	PRON
fnp-5883	303	6	prevented	prevent	VERB
fnp-5883	303	7	the	the	DET
fnp-5883	303	8	visualization	visualization	NOUN
fnp-5883	303	9	of	of	ADP
fnp-5883	303	10	structures	structure	NOUN
fnp-5883	303	11	in	in	ADP
fnp-5883	303	12	the	the	DET
fnp-5883	303	13	freeze	freeze	NOUN
fnp-5883	303	14	-	-	PUNCT
fnp-5883	303	15	substituted	substitute	VERB
fnp-5883	303	16	state	state	NOUN
fnp-5883	303	17	may	may	AUX
fnp-5883	303	18	have	have	AUX
fnp-5883	303	19	been	be	AUX
fnp-5883	303	20	reversed	reverse	VERB
fnp-5883	303	21	by	by	ADP
fnp-5883	303	22	thawing	thaw	VERB
fnp-5883	303	23	.	.	PUNCT
fnp-5883	304	1	a	a	DET
fnp-5883	304	2	separate	separate	ADJ
fnp-5883	304	3	study	study	NOUN
fnp-5883	304	4	examined	examine	VERB
fnp-5883	304	5	electron	electron	NOUN
fnp-5883	304	6	microscopy	microscopy	NOUN
fnp-5883	304	7	in	in	ADP
fnp-5883	304	8	1	1	NUM
fnp-5883	304	9	 	 	SPACE
fnp-5883	304	10	cm	cm	NOUN
fnp-5883	304	11	thick	thick	ADJ
fnp-5883	304	12	sections	section	NOUN
fnp-5883	304	13	of	of	ADP
fnp-5883	304	14	human	human	ADJ
fnp-5883	304	15	brain	brain	NOUN
fnp-5883	304	16	tissue	tissue	NOUN
fnp-5883	304	17	that	that	PRON
fnp-5883	304	18	were	be	AUX
fnp-5883	304	19	frozen	freeze	VERB
fnp-5883	304	20	between	between	ADP
fnp-5883	304	21	dry	dry	ADJ
fnp-5883	304	22	ice	ice	NOUN
fnp-5883	304	23	-	-	PUNCT
fnp-5883	304	24	cooled	cool	VERB
fnp-5883	304	25	aluminum	aluminum	NOUN
fnp-5883	304	26	plates	plate	NOUN
fnp-5883	304	27	and	and	CCONJ
fnp-5883	304	28	immersion	immersion	NOUN
fnp-5883	304	29	-	-	PUNCT
fnp-5883	304	30	fixed	fix	VERB
fnp-5883	304	31	during	during	ADP
fnp-5883	304	32	thawing	thaw	VERB
fnp-5883	304	33	(	(	PUNCT
fnp-5883	304	34	nochlin	nochlin	PROPN
fnp-5883	304	35	et	et	PROPN
fnp-5883	304	36	al	al	PROPN
fnp-5883	304	37	.	.	PROPN
fnp-5883	304	38	,	,	PUNCT
fnp-5883	304	39	1993	1993	NUM
fnp-5883	304	40	)	)	PUNCT
fnp-5883	304	41	.	.	PUNCT
fnp-5883	305	1	the	the	DET
fnp-5883	305	2	study	study	NOUN
fnp-5883	305	3	found	find	VERB
fnp-5883	305	4	that	that	SCONJ
fnp-5883	305	5	the	the	DET
fnp-5883	305	6	preservation	preservation	NOUN
fnp-5883	305	7	quality	quality	NOUN
fnp-5883	305	8	in	in	ADP
fnp-5883	305	9	most	most	ADJ
fnp-5883	305	10	images	image	NOUN
fnp-5883	305	11	was	be	AUX
fnp-5883	305	12	similar	similar	ADJ
fnp-5883	305	13	to	to	ADP
fnp-5883	305	14	that	that	PRON
fnp-5883	305	15	in	in	ADP
fnp-5883	305	16	non	non	ADJ
fnp-5883	305	17	-	-	ADJ
fnp-5883	305	18	frozen	frozen	ADJ
fnp-5883	305	19	brain	brain	NOUN
fnp-5883	305	20	tissue	tissue	NOUN
fnp-5883	305	21	,	,	PUNCT
fnp-5883	305	22	with	with	ADP
fnp-5883	305	23	the	the	DET
fnp-5883	305	24	different	different	ADJ
fnp-5883	305	25	cell	cell	NOUN
fnp-5883	305	26	types	type	NOUN
fnp-5883	305	27	and	and	CCONJ
fnp-5883	305	28	processes	process	NOUN
fnp-5883	305	29	easily	easily	ADV
fnp-5883	305	30	able	able	ADJ
fnp-5883	305	31	to	to	PART
fnp-5883	305	32	be	be	AUX
fnp-5883	305	33	identified	identify	VERB
fnp-5883	305	34	.	.	PUNCT
fnp-5883	306	1	this	this	DET
fnp-5883	306	2	finding	finding	NOUN
fnp-5883	306	3	contradicts	contradict	VERB
fnp-5883	306	4	the	the	DET
fnp-5883	306	5	literature	literature	NOUN
fnp-5883	306	6	using	use	VERB
fnp-5883	306	7	cryofixation	cryofixation	NOUN
fnp-5883	306	8	and	and	CCONJ
fnp-5883	306	9	freeze	freeze	VERB
fnp-5883	306	10	substitution	substitution	NOUN
fnp-5883	306	11	followed	follow	VERB
fnp-5883	306	12	by	by	ADP
fnp-5883	306	13	electron	electron	NOUN
fnp-5883	306	14	microscopy	microscopy	NOUN
fnp-5883	306	15	,	,	PUNCT
fnp-5883	306	16	which	which	PRON
fnp-5883	306	17	,	,	PUNCT
fnp-5883	306	18	as	as	SCONJ
fnp-5883	306	19	discussed	discuss	VERB
fnp-5883	306	20	above	above	ADV
fnp-5883	306	21	,	,	PUNCT
fnp-5883	306	22	generally	generally	ADV
fnp-5883	306	23	finds	find	VERB
fnp-5883	306	24	severe	severe	ADJ
fnp-5883	306	25	histologic	histologic	ADJ
fnp-5883	306	26	damage	damage	NOUN
fnp-5883	306	27	in	in	ADP
fnp-5883	306	28	regions	region	NOUN
fnp-5883	306	29	deep	deep	ADV
fnp-5883	306	30	enough	enough	ADV
fnp-5883	306	31	to	to	PART
fnp-5883	306	32	be	be	AUX
fnp-5883	306	33	affected	affect	VERB
fnp-5883	306	34	by	by	ADP
fnp-5883	306	35	ice	ice	NOUN
fnp-5883	306	36	crystals	crystal	NOUN
fnp-5883	306	37	.	.	PUNCT
fnp-5883	307	1	one	one	NUM
fnp-5883	307	2	source	source	NOUN
fnp-5883	307	3	discusses	discuss	VERB
fnp-5883	307	4	ultrastructural	ultrastructural	ADJ
fnp-5883	307	5	preservation	preservation	NOUN
fnp-5883	307	6	quality	quality	NOUN
fnp-5883	307	7	after	after	ADP
fnp-5883	307	8	the	the	DET
fnp-5883	307	9	cryopreservation	cryopreservation	NOUN
fnp-5883	307	10	of	of	ADP
fnp-5883	307	11	several	several	ADJ
fnp-5883	307	12	different	different	ADJ
fnp-5883	307	13	cell	cell	NOUN
fnp-5883	307	14	types	type	NOUN
fnp-5883	307	15	,	,	PUNCT
fnp-5883	307	16	especially	especially	ADV
fnp-5883	307	17	hepatic	hepatic	ADJ
fnp-5883	307	18	cells	cell	NOUN
fnp-5883	307	19	,	,	PUNCT
fnp-5883	307	20	but	but	CCONJ
fnp-5883	307	21	also	also	ADV
fnp-5883	307	22	with	with	ADP
fnp-5883	307	23	some	some	DET
fnp-5883	307	24	discussion	discussion	NOUN
fnp-5883	307	25	of	of	ADP
fnp-5883	307	26	brain	brain	NOUN
fnp-5883	307	27	cells	cell	NOUN
fnp-5883	307	28	(	(	PUNCT
fnp-5883	307	29	trump	trump	VERB
fnp-5883	307	30	et	et	PROPN
fnp-5883	307	31	al	al	PROPN
fnp-5883	307	32	.	.	PROPN
fnp-5883	307	33	,	,	PUNCT
fnp-5883	307	34	1965	1965	NUM
fnp-5883	307	35	)	)	PUNCT
fnp-5883	307	36	.	.	PUNCT
fnp-5883	308	1	the	the	DET
fnp-5883	308	2	authors	author	NOUN
fnp-5883	308	3	note	note	VERB
fnp-5883	308	4	that	that	SCONJ
fnp-5883	308	5	slow	slow	ADJ
fnp-5883	308	6	freezing	freezing	NOUN
fnp-5883	308	7	causes	cause	VERB
fnp-5883	308	8	significant	significant	ADJ
fnp-5883	308	9	extracellular	extracellular	ADJ
fnp-5883	308	10	ice	ice	NOUN
fnp-5883	308	11	damage	damage	NOUN
fnp-5883	308	12	,	,	PUNCT
fnp-5883	308	13	but	but	CCONJ
fnp-5883	308	14	that	that	SCONJ
fnp-5883	308	15	there	there	PRON
fnp-5883	308	16	is	be	VERB
fnp-5883	308	17	a	a	DET
fnp-5883	308	18	significant	significant	ADJ
fnp-5883	308	19	amount	amount	NOUN
fnp-5883	308	20	of	of	ADP
fnp-5883	308	21	what	what	PRON
fnp-5883	308	22	they	they	PRON
fnp-5883	308	23	call	call	VERB
fnp-5883	308	24	“	"	PUNCT
fnp-5883	308	25	structural	structural	ADJ
fnp-5883	308	26	restitution	restitution	NOUN
fnp-5883	308	27	”	"	PUNCT
fnp-5883	308	28	on	on	ADP
fnp-5883	308	29	thawing	thaw	VERB
fnp-5883	308	30	when	when	SCONJ
fnp-5883	308	31	cells	cell	NOUN
fnp-5883	308	32	are	be	AUX
fnp-5883	308	33	preserved	preserve	VERB
fnp-5883	308	34	with	with	ADP
fnp-5883	308	35	slow	slow	ADJ
fnp-5883	308	36	freezing	freezing	NOUN
fnp-5883	308	37	.	.	PUNCT
fnp-5883	309	1	they	they	PRON
fnp-5883	309	2	primarily	primarily	ADV
fnp-5883	309	3	discuss	discuss	VERB
fnp-5883	309	4	intracellular	intracellular	ADJ
fnp-5883	309	5	features	feature	NOUN
fnp-5883	309	6	such	such	ADJ
fnp-5883	309	7	as	as	ADP
fnp-5883	309	8	mitochondria	mitochondria	NOUN
fnp-5883	309	9	,	,	PUNCT
fnp-5883	309	10	which	which	PRON
fnp-5883	309	11	are	be	AUX
fnp-5883	309	12	more	more	ADV
fnp-5883	309	13	likely	likely	ADJ
fnp-5883	309	14	to	to	PART
fnp-5883	309	15	be	be	AUX
fnp-5883	309	16	protected	protect	VERB
fnp-5883	309	17	by	by	ADP
fnp-5883	309	18	the	the	DET
fnp-5883	309	19	predominantly	predominantly	ADV
fnp-5883	309	20	extracellular	extracellular	ADJ
fnp-5883	309	21	ice	ice	NOUN
fnp-5883	309	22	formation	formation	NOUN
fnp-5883	309	23	that	that	PRON
fnp-5883	309	24	will	will	AUX
fnp-5883	309	25	occur	occur	VERB
fnp-5883	309	26	during	during	ADP
fnp-5883	309	27	slow	slow	ADJ
fnp-5883	309	28	freezing	freezing	NOUN
fnp-5883	309	29	.	.	PUNCT
fnp-5883	310	1	also	also	ADV
fnp-5883	310	2	,	,	PUNCT
fnp-5883	310	3	the	the	DET
fnp-5883	310	4	extent	extent	NOUN
fnp-5883	310	5	to	to	PART
fnp-5883	310	6	which	which	PRON
fnp-5883	310	7	any	any	DET
fnp-5883	310	8	such	such	ADJ
fnp-5883	310	9	structural	structural	ADJ
fnp-5883	310	10	restitution	restitution	NOUN
fnp-5883	310	11	yields	yield	VERB
fnp-5883	310	12	the	the	DET
fnp-5883	310	13	same	same	ADJ
fnp-5883	310	14	original	original	ADJ
fnp-5883	310	15	morphology	morphology	NOUN
fnp-5883	310	16	present	present	ADJ
fnp-5883	310	17	prior	prior	ADV
fnp-5883	310	18	to	to	ADP
fnp-5883	310	19	freezing	freezing	NOUN
fnp-5883	310	20	is	be	AUX
fnp-5883	310	21	not	not	PART
fnp-5883	310	22	entirely	entirely	ADV
fnp-5883	310	23	clear	clear	ADJ
fnp-5883	310	24	.	.	PUNCT
fnp-5883	311	1	taken	take	VERB
fnp-5883	311	2	together	together	ADV
fnp-5883	311	3	,	,	PUNCT
fnp-5883	311	4	the	the	DET
fnp-5883	311	5	issue	issue	NOUN
fnp-5883	311	6	of	of	ADP
fnp-5883	311	7	histologic	histologic	ADJ
fnp-5883	311	8	damage	damage	NOUN
fnp-5883	311	9	during	during	ADP
fnp-5883	311	10	rewarming	rewarming	NOUN
fnp-5883	311	11	,	,	PUNCT
fnp-5883	311	12	and	and	CCONJ
fnp-5883	311	13	how	how	SCONJ
fnp-5883	311	14	to	to	PART
fnp-5883	311	15	best	well	ADV
fnp-5883	311	16	decrease	decrease	VERB
fnp-5883	311	17	or	or	CCONJ
fnp-5883	311	18	prevent	prevent	VERB
fnp-5883	311	19	it	it	PRON
fnp-5883	311	20	,	,	PUNCT
fnp-5883	311	21	appears	appear	VERB
fnp-5883	311	22	to	to	PART
fnp-5883	311	23	be	be	AUX
fnp-5883	311	24	an	an	DET
fnp-5883	311	25	unresolved	unresolved	ADJ
fnp-5883	311	26	one	one	NOUN
fnp-5883	311	27	.	.	PUNCT
fnp-5883	312	1	there	there	PRON
fnp-5883	312	2	is	be	VERB
fnp-5883	312	3	a	a	DET
fnp-5883	312	4	need	need	NOUN
fnp-5883	312	5	for	for	ADP
fnp-5883	312	6	more	more	ADJ
fnp-5883	312	7	research	research	NOUN
fnp-5883	312	8	comparing	compare	VERB
fnp-5883	312	9	different	different	ADJ
fnp-5883	312	10	cooling	cooling	NOUN
fnp-5883	312	11	and	and	CCONJ
fnp-5883	312	12	rewarming	rewarming	NOUN
fnp-5883	312	13	methods	method	NOUN
fnp-5883	312	14	and	and	CCONJ
fnp-5883	312	15	for	for	ADP
fnp-5883	312	16	the	the	DET
fnp-5883	312	17	development	development	NOUN
fnp-5883	312	18	of	of	ADP
fnp-5883	312	19	more	more	ADJ
fnp-5883	312	20	objective	objective	ADJ
fnp-5883	312	21	histologic	histologic	ADJ
fnp-5883	312	22	metrics	metric	NOUN
fnp-5883	312	23	to	to	PART
fnp-5883	312	24	compare	compare	VERB
fnp-5883	312	25	across	across	ADP
fnp-5883	312	26	methods	method	NOUN
fnp-5883	312	27	and	and	CCONJ
fnp-5883	312	28	studies	study	NOUN
fnp-5883	312	29	.	.	PUNCT
fnp-5883	313	1	tissue	tissue	NOUN
fnp-5883	313	2	freezing	freezing	NOUN
fnp-5883	313	3	in	in	ADP
fnp-5883	313	4	brain	brain	NOUN
fnp-5883	313	5	banking	banking	NOUN
fnp-5883	313	6	perhaps	perhaps	ADV
fnp-5883	313	7	the	the	DET
fnp-5883	313	8	simplest	simple	ADJ
fnp-5883	313	9	way	way	NOUN
fnp-5883	313	10	of	of	ADP
fnp-5883	313	11	freezing	freeze	VERB
fnp-5883	313	12	tissue	tissue	NOUN
fnp-5883	313	13	in	in	ADP
fnp-5883	313	14	brain	brain	NOUN
fnp-5883	313	15	banking	banking	NOUN
fnp-5883	313	16	is	be	AUX
fnp-5883	313	17	to	to	PART
fnp-5883	313	18	isolate	isolate	VERB
fnp-5883	313	19	the	the	DET
fnp-5883	313	20	whole	whole	ADJ
fnp-5883	313	21	brain	brain	NOUN
fnp-5883	313	22	or	or	CCONJ
fnp-5883	313	23	a	a	DET
fnp-5883	313	24	brain	brain	NOUN
fnp-5883	313	25	hemisphere	hemisphere	ADV
fnp-5883	313	26	and	and	CCONJ
fnp-5883	313	27	place	place	VERB
fnp-5883	313	28	it	it	PRON
fnp-5883	313	29	in	in	ADP
fnp-5883	313	30	a	a	DET
fnp-5883	313	31	freezer	freezer	NOUN
fnp-5883	313	32	.	.	PUNCT
fnp-5883	314	1	this	this	DET
fnp-5883	314	2	method	method	NOUN
fnp-5883	314	3	was	be	AUX
fnp-5883	314	4	used	use	VERB
fnp-5883	314	5	early	early	ADV
fnp-5883	314	6	in	in	ADP
fnp-5883	314	7	the	the	DET
fnp-5883	314	8	history	history	NOUN
fnp-5883	314	9	of	of	ADP
fnp-5883	314	10	brain	brain	NOUN
fnp-5883	314	11	banking	banking	NOUN
fnp-5883	314	12	(	(	PUNCT
fnp-5883	314	13	bird	bird	NOUN
fnp-5883	314	14	,	,	PUNCT
fnp-5883	314	15	1980	1980	NUM
fnp-5883	314	16	)	)	PUNCT
fnp-5883	314	17	.	.	PUNCT
fnp-5883	315	1	however	however	ADV
fnp-5883	315	2	,	,	PUNCT
fnp-5883	315	3	this	this	DET
fnp-5883	315	4	method	method	NOUN
fnp-5883	315	5	has	have	AUX
fnp-5883	315	6	been	be	AUX
fnp-5883	315	7	found	find	VERB
fnp-5883	315	8	to	to	PART
fnp-5883	315	9	lead	lead	VERB
fnp-5883	315	10	to	to	ADP
fnp-5883	315	11	severe	severe	ADJ
fnp-5883	315	12	ice	ice	NOUN
fnp-5883	315	13	artifacts	artifact	NOUN
fnp-5883	315	14	on	on	ADP
fnp-5883	315	15	histology	histology	NOUN
fnp-5883	315	16	,	,	PUNCT
fnp-5883	315	17	consistent	consistent	ADJ
fnp-5883	315	18	with	with	ADP
fnp-5883	315	19	the	the	DET
fnp-5883	315	20	slow	slow	ADJ
fnp-5883	315	21	cooling	cooling	NOUN
fnp-5883	315	22	and	and	CCONJ
fnp-5883	315	23	rewarming	rewarming	NOUN
fnp-5883	315	24	rates	rate	NOUN
fnp-5883	315	25	expected	expect	VERB
fnp-5883	315	26	when	when	SCONJ
fnp-5883	315	27	freezing	freeze	VERB
fnp-5883	315	28	and	and	CCONJ
fnp-5883	315	29	thawing	thaw	VERB
fnp-5883	315	30	a	a	DET
fnp-5883	315	31	large	large	ADJ
fnp-5883	315	32	brain	brain	NOUN
fnp-5883	315	33	specimen	speciman	NOUN
fnp-5883	315	34	in	in	ADP
fnp-5883	315	35	this	this	DET
fnp-5883	315	36	manner	manner	NOUN
fnp-5883	315	37	(	(	PUNCT
fnp-5883	315	38	tourtellotte	tourtellotte	NOUN
fnp-5883	315	39	et	et	PROPN
fnp-5883	315	40	al	al	PROPN
fnp-5883	315	41	.	.	PROPN
fnp-5883	315	42	,	,	PUNCT
fnp-5883	315	43	1993	1993	NUM
fnp-5883	315	44	;	;	PUNCT
fnp-5883	315	45	vonsattel	vonsattel	NOUN
fnp-5883	315	46	et	et	PROPN
fnp-5883	315	47	al	al	PROPN
fnp-5883	315	48	.	.	PROPN
fnp-5883	315	49	,	,	PUNCT
fnp-5883	315	50	1995	1995	NUM
fnp-5883	315	51	,	,	PUNCT
fnp-5883	315	52	2008	2008	NUM
fnp-5883	315	53	)	)	PUNCT
fnp-5883	315	54	.	.	PUNCT
fnp-5883	316	1	cryopreserving	cryopreserve	VERB
fnp-5883	316	2	very	very	ADV
fnp-5883	316	3	large	large	ADJ
fnp-5883	316	4	samples	sample	NOUN
fnp-5883	316	5	is	be	AUX
fnp-5883	316	6	also	also	ADV
fnp-5883	316	7	impractical	impractical	ADJ
fnp-5883	316	8	for	for	ADP
fnp-5883	316	9	most	most	ADJ
fnp-5883	316	10	research	research	NOUN
fnp-5883	316	11	applications	application	NOUN
fnp-5883	316	12	,	,	PUNCT
fnp-5883	316	13	because	because	SCONJ
fnp-5883	316	14	accessing	access	VERB
fnp-5883	316	15	small	small	ADJ
fnp-5883	316	16	parts	part	NOUN
fnp-5883	316	17	of	of	ADP
fnp-5883	316	18	the	the	DET
fnp-5883	316	19	brain	brain	NOUN
fnp-5883	316	20	for	for	ADP
fnp-5883	316	21	study	study	NOUN
fnp-5883	316	22	would	would	AUX
fnp-5883	316	23	require	require	VERB
fnp-5883	316	24	several	several	ADJ
fnp-5883	316	25	freeze	freeze	NOUN
fnp-5883	316	26	-	-	PUNCT
fnp-5883	316	27	thaw	thaw	NOUN
fnp-5883	316	28	cycles	cycle	NOUN
fnp-5883	316	29	,	,	PUNCT
fnp-5883	316	30	leading	lead	VERB
fnp-5883	316	31	to	to	ADP
fnp-5883	316	32	even	even	ADV
fnp-5883	316	33	more	more	ADV
fnp-5883	316	34	severe	severe	ADJ
fnp-5883	316	35	morphological	morphological	ADJ
fnp-5883	316	36	damage	damage	NOUN
fnp-5883	316	37	.	.	PUNCT
fnp-5883	317	1	instead	instead	ADV
fnp-5883	317	2	,	,	PUNCT
fnp-5883	317	3	what	what	PRON
fnp-5883	317	4	appears	appear	VERB
fnp-5883	317	5	to	to	PART
fnp-5883	317	6	be	be	AUX
fnp-5883	317	7	the	the	DET
fnp-5883	317	8	most	most	ADV
fnp-5883	317	9	common	common	ADJ
fnp-5883	317	10	method	method	NOUN
fnp-5883	317	11	in	in	ADP
fnp-5883	317	12	modern	modern	ADJ
fnp-5883	317	13	brain	brain	NOUN
fnp-5883	317	14	banking	banking	NOUN
fnp-5883	317	15	is	be	AUX
fnp-5883	317	16	to	to	PART
fnp-5883	317	17	section	section	VERB
fnp-5883	317	18	fresh	fresh	ADJ
fnp-5883	317	19	brain	brain	NOUN
fnp-5883	317	20	tissue	tissue	NOUN
fnp-5883	317	21	and	and	CCONJ
fnp-5883	317	22	use	use	VERB
fnp-5883	317	23	pre	pre	ADJ
fnp-5883	317	24	-	-	ADJ
fnp-5883	317	25	cooled	cooled	ADJ
fnp-5883	317	26	metal	metal	NOUN
fnp-5883	317	27	plates	plate	NOUN
fnp-5883	317	28	to	to	PART
fnp-5883	317	29	freeze	freeze	VERB
fnp-5883	317	30	the	the	DET
fnp-5883	317	31	slices	slice	NOUN
fnp-5883	317	32	(	(	PUNCT
fnp-5883	317	33	vonsattel	vonsattel	NOUN
fnp-5883	317	34	et	et	PROPN
fnp-5883	317	35	al	al	PROPN
fnp-5883	317	36	.	.	PROPN
fnp-5883	317	37	,	,	PUNCT
fnp-5883	317	38	2008	2008	NUM
fnp-5883	317	39	)	)	PUNCT
fnp-5883	317	40	.	.	PUNCT
fnp-5883	318	1	to	to	PART
fnp-5883	318	2	assess	assess	VERB
fnp-5883	318	3	the	the	DET
fnp-5883	318	4	degree	degree	NOUN
fnp-5883	318	5	of	of	ADP
fnp-5883	318	6	morphological	morphological	ADJ
fnp-5883	318	7	preservation	preservation	NOUN
fnp-5883	318	8	in	in	ADP
fnp-5883	318	9	a	a	DET
fnp-5883	318	10	routinely	routinely	ADV
fnp-5883	318	11	banked	banked	ADJ
fnp-5883	318	12	brain	brain	NOUN
fnp-5883	318	13	preserved	preserve	VERB
fnp-5883	318	14	via	via	ADP
fnp-5883	318	15	freezing	freeze	VERB
fnp-5883	318	16	in	in	ADP
fnp-5883	318	17	such	such	DET
fnp-5883	318	18	a	a	DET
fnp-5883	318	19	manner	manner	NOUN
fnp-5883	318	20	,	,	PUNCT
fnp-5883	318	21	we	we	PRON
fnp-5883	318	22	examined	examine	VERB
fnp-5883	318	23	images	image	NOUN
fnp-5883	318	24	from	from	ADP
fnp-5883	318	25	cortical	cortical	ADJ
fnp-5883	318	26	tissue	tissue	NOUN
fnp-5883	318	27	that	that	PRON
fnp-5883	318	28	was	be	AUX
fnp-5883	318	29	initially	initially	ADV
fnp-5883	318	30	preserved	preserve	VERB
fnp-5883	318	31	by	by	ADP
fnp-5883	318	32	freezing	freeze	VERB
fnp-5883	318	33	between	between	ADP
fnp-5883	318	34	metal	metal	NOUN
fnp-5883	318	35	plates	plate	NOUN
fnp-5883	318	36	pre	pre	ADJ
fnp-5883	318	37	-	-	VERB
fnp-5883	318	38	cooled	cooled	ADJ
fnp-5883	318	39	in	in	ADP
fnp-5883	318	40	a	a	DET
fnp-5883	318	41	‑80	‑80	NOUN
fnp-5883	318	42	 	 	SPACE
fnp-5883	318	43	°	°	ADP
fnp-5883	318	44	c	c	NOUN
fnp-5883	318	45	freezer	freezer	NOUN
fnp-5883	318	46	and	and	CCONJ
fnp-5883	318	47	then	then	ADV
fnp-5883	318	48	rewarmed	rewarme	VERB
fnp-5883	318	49	in	in	ADP
fnp-5883	318	50	cold	cold	ADJ
fnp-5883	318	51	fixative	fixative	NOUN
fnp-5883	318	52	.	.	PUNCT
fnp-5883	319	1	we	we	PRON
fnp-5883	319	2	found	find	VERB
fnp-5883	319	3	that	that	SCONJ
fnp-5883	319	4	in	in	ADP
fnp-5883	319	5	this	this	DET
fnp-5883	319	6	sample	sample	NOUN
fnp-5883	319	7	,	,	PUNCT
fnp-5883	319	8	ice	ice	NOUN
fnp-5883	319	9	artifacts	artifact	NOUN
fnp-5883	319	10	are	be	AUX
fnp-5883	319	11	readily	readily	ADV
fnp-5883	319	12	apparent	apparent	ADJ
fnp-5883	319	13	in	in	ADP
fnp-5883	319	14	the	the	DET
fnp-5883	319	15	frozen	frozen	ADJ
fnp-5883	319	16	tissue	tissue	NOUN
fnp-5883	319	17	compared	compare	VERB
fnp-5883	319	18	to	to	ADP
fnp-5883	319	19	the	the	DET
fnp-5883	319	20	immersion	immersion	NOUN
fnp-5883	319	21	-	-	PUNCT
fnp-5883	319	22	fixed	fix	VERB
fnp-5883	319	23	tissue	tissue	NOUN
fnp-5883	319	24	(	(	PUNCT
fnp-5883	319	25	figure	figure	NOUN
fnp-5883	319	26	 	 	SPACE
fnp-5883	319	27	2	2	NUM
fnp-5883	319	28	)	)	PUNCT
fnp-5883	319	29	.	.	PUNCT
fnp-5883	320	1	the	the	DET
fnp-5883	320	2	cells	cell	NOUN
fnp-5883	320	3	,	,	PUNCT
fnp-5883	320	4	especially	especially	ADV
fnp-5883	320	5	those	those	PRON
fnp-5883	320	6	in	in	ADP
fnp-5883	320	7	the	the	DET
fnp-5883	320	8	grey	grey	ADJ
fnp-5883	320	9	matter	matter	NOUN
fnp-5883	320	10	,	,	PUNCT
fnp-5883	320	11	are	be	AUX
fnp-5883	320	12	shrunken	shrunken	ADJ
fnp-5883	320	13	and	and	CCONJ
fnp-5883	320	14	mechanically	mechanically	ADV
fnp-5883	320	15	squashed	squash	VERB
fnp-5883	320	16	into	into	ADP
fnp-5883	320	17	the	the	DET
fnp-5883	320	18	pockets	pocket	NOUN
fnp-5883	320	19	of	of	ADP
fnp-5883	320	20	non	non	ADJ
fnp-5883	320	21	-	-	ADJ
fnp-5883	320	22	frozen	frozen	ADJ
fnp-5883	320	23	tissue	tissue	NOUN
fnp-5883	320	24	between	between	ADP
fnp-5883	320	25	large	large	ADJ
fnp-5883	320	26	areas	area	NOUN
fnp-5883	320	27	of	of	ADP
fnp-5883	320	28	empty	empty	ADJ
fnp-5883	320	29	space	space	NOUN
fnp-5883	320	30	indicative	indicative	ADJ
fnp-5883	320	31	of	of	ADP
fnp-5883	320	32	ice	ice	NOUN
fnp-5883	320	33	formation	formation	NOUN
fnp-5883	320	34	.	.	PUNCT
fnp-5883	321	1	in	in	ADP
fnp-5883	321	2	this	this	DET
fnp-5883	321	3	example	example	NOUN
fnp-5883	321	4	,	,	PUNCT
fnp-5883	321	5	the	the	DET
fnp-5883	321	6	degree	degree	NOUN
fnp-5883	321	7	of	of	ADP
fnp-5883	321	8	ice	ice	NOUN
fnp-5883	321	9	artifact	artifact	NOUN
fnp-5883	321	10	in	in	ADP
fnp-5883	321	11	white	white	ADJ
fnp-5883	321	12	matter	matter	NOUN
fnp-5883	321	13	appears	appear	VERB
fnp-5883	321	14	to	to	PART
fnp-5883	321	15	be	be	AUX
fnp-5883	321	16	relatively	relatively	ADV
fnp-5883	321	17	less	less	ADV
fnp-5883	321	18	severe	severe	ADJ
fnp-5883	321	19	than	than	ADP
fnp-5883	321	20	in	in	ADP
fnp-5883	321	21	the	the	DET
fnp-5883	321	22	grey	grey	NOUN
fnp-5883	321	23	matter	matter	NOUN
fnp-5883	321	24	.	.	PUNCT
fnp-5883	322	1	notably	notably	ADV
fnp-5883	322	2	,	,	PUNCT
fnp-5883	322	3	myelin	myelin	NOUN
fnp-5883	322	4	has	have	AUX
fnp-5883	322	5	previously	previously	ADV
fnp-5883	322	6	been	be	AUX
fnp-5883	322	7	reported	report	VERB
fnp-5883	322	8	to	to	PART
fnp-5883	322	9	be	be	AUX
fnp-5883	322	10	relatively	relatively	ADV
fnp-5883	322	11	resistant	resistant	ADJ
fnp-5883	322	12	to	to	ADP
fnp-5883	322	13	freezing	freeze	VERB
fnp-5883	322	14	damage	damage	NOUN
fnp-5883	322	15	(	(	PUNCT
fnp-5883	322	16	möbius	möbius	NOUN
fnp-5883	322	17	et	et	PROPN
fnp-5883	322	18	al	al	PROPN
fnp-5883	322	19	.	.	PROPN
fnp-5883	322	20	,	,	PUNCT
fnp-5883	322	21	2010	2010	NUM
fnp-5883	322	22	)	)	PUNCT
fnp-5883	322	23	.	.	PUNCT
fnp-5883	323	1	figure	figure	NOUN
fnp-5883	323	2	 	 	SPACE
fnp-5883	323	3	2	2	NUM
fnp-5883	323	4	.	.	NOUN
fnp-5883	323	5	example	example	NOUN
fnp-5883	323	6	images	image	NOUN
fnp-5883	323	7	showing	show	VERB
fnp-5883	323	8	ice	ice	NOUN
fnp-5883	323	9	artifacts	artifact	NOUN
fnp-5883	323	10	in	in	ADP
fnp-5883	323	11	frozen	frozen	ADJ
fnp-5883	323	12	-	-	PUNCT
fnp-5883	323	13	thawed	thaw	VERB
fnp-5883	323	14	brain	brain	NOUN
fnp-5883	323	15	tissue	tissue	NOUN
fnp-5883	323	16	.	.	PUNCT
fnp-5883	324	1	this	this	DET
fnp-5883	324	2	brain	brain	NOUN
fnp-5883	324	3	tissue	tissue	NOUN
fnp-5883	324	4	is	be	AUX
fnp-5883	324	5	from	from	ADP
fnp-5883	324	6	a	a	DET
fnp-5883	324	7	76	76	NUM
fnp-5883	324	8	-	-	PUNCT
fnp-5883	324	9	year	year	NOUN
fnp-5883	324	10	-	-	PUNCT
fnp-5883	324	11	old	old	ADJ
fnp-5883	324	12	female	female	ADJ
fnp-5883	324	13	brain	brain	NOUN
fnp-5883	324	14	donor	donor	NOUN
fnp-5883	324	15	with	with	ADP
fnp-5883	324	16	a	a	DET
fnp-5883	324	17	postmortem	postmortem	NOUN
fnp-5883	324	18	interval	interval	NOUN
fnp-5883	324	19	of	of	ADP
fnp-5883	324	20	70.5	70.5	NUM
fnp-5883	324	21	hours	hour	NOUN
fnp-5883	324	22	prior	prior	ADV
fnp-5883	324	23	to	to	ADP
fnp-5883	324	24	preservation	preservation	NOUN
fnp-5883	324	25	.	.	PUNCT
fnp-5883	325	1	the	the	DET
fnp-5883	325	2	frozen	frozen	ADJ
fnp-5883	325	3	tissue	tissue	NOUN
fnp-5883	325	4	was	be	AUX
fnp-5883	325	5	from	from	ADP
fnp-5883	325	6	approximately	approximately	ADV
fnp-5883	325	7	0.5	0.5	NUM
fnp-5883	325	8	 	 	SPACE
fnp-5883	325	9	cm	cm	NOUN
fnp-5883	325	10	fresh	fresh	ADJ
fnp-5883	325	11	sections	section	NOUN
fnp-5883	325	12	frozen	freeze	VERB
fnp-5883	325	13	between	between	ADP
fnp-5883	325	14	teflon	teflon	NOUN
fnp-5883	325	15	-	-	PUNCT
fnp-5883	325	16	coated	coat	VERB
fnp-5883	325	17	metal	metal	NOUN
fnp-5883	325	18	plates	plate	NOUN
fnp-5883	325	19	cooled	cool	VERB
fnp-5883	325	20	at	at	ADP
fnp-5883	325	21	‑80	‑80	NOUN
fnp-5883	325	22	 	 	SPACE
fnp-5883	325	23	°	°	ADP
fnp-5883	325	24	c	c	NOUN
fnp-5883	325	25	.	.	PUNCT
fnp-5883	326	1	the	the	DET
fnp-5883	326	2	frozen	frozen	ADJ
fnp-5883	326	3	tissue	tissue	NOUN
fnp-5883	326	4	was	be	AUX
fnp-5883	326	5	stored	store	VERB
fnp-5883	326	6	at	at	ADP
fnp-5883	326	7	‑80	‑80	NOUN
fnp-5883	326	8	 	 	SPACE
fnp-5883	326	9	°	°	ADP
fnp-5883	326	10	c	c	NOUN
fnp-5883	326	11	for	for	ADP
fnp-5883	326	12	over	over	ADP
fnp-5883	326	13	5	5	NUM
fnp-5883	326	14	years	year	NOUN
fnp-5883	326	15	before	before	ADP
fnp-5883	326	16	being	be	AUX
fnp-5883	326	17	thawed	thaw	VERB
fnp-5883	326	18	in	in	ADP
fnp-5883	326	19	cold	cold	ADJ
fnp-5883	326	20	formalin	formalin	NOUN
fnp-5883	326	21	.	.	PUNCT
fnp-5883	327	1	ice	ice	NOUN
fnp-5883	327	2	artifacts	artifact	NOUN
fnp-5883	327	3	in	in	ADP
fnp-5883	327	4	frozen	frozen	ADJ
fnp-5883	327	5	-	-	PUNCT
fnp-5883	327	6	thawed	thaw	VERB
fnp-5883	327	7	tissue	tissue	NOUN
fnp-5883	327	8	(	(	PUNCT
fnp-5883	327	9	b	b	NOUN
fnp-5883	327	10	,	,	PUNCT
fnp-5883	327	11	d	d	PROPN
fnp-5883	327	12	,	,	PUNCT
fnp-5883	327	13	f	f	X
fnp-5883	327	14	,	,	PUNCT
fnp-5883	327	15	h	h	NOUN
fnp-5883	327	16	)	)	PUNCT
fnp-5883	327	17	can	can	AUX
fnp-5883	327	18	be	be	AUX
fnp-5883	327	19	observed	observe	VERB
fnp-5883	327	20	when	when	SCONJ
fnp-5883	327	21	compared	compare	VERB
fnp-5883	327	22	with	with	ADP
fnp-5883	327	23	immersion	immersion	NOUN
fnp-5883	327	24	fixed	fix	VERB
fnp-5883	327	25	tissue	tissue	NOUN
fnp-5883	327	26	(	(	PUNCT
fnp-5883	327	27	a	a	PRON
fnp-5883	327	28	,	,	PUNCT
fnp-5883	327	29	c	c	NOUN
fnp-5883	327	30	,	,	PUNCT
fnp-5883	327	31	e	e	NOUN
fnp-5883	327	32	,	,	PUNCT
fnp-5883	327	33	g	g	NOUN
fnp-5883	327	34	)	)	PUNCT
fnp-5883	327	35	even	even	ADV
fnp-5883	327	36	at	at	ADP
fnp-5883	327	37	low	low	ADJ
fnp-5883	327	38	magnification	magnification	NOUN
fnp-5883	327	39	(	(	PUNCT
fnp-5883	327	40	a	a	DET
fnp-5883	327	41	,	,	PUNCT
fnp-5883	327	42	b	b	NOUN
fnp-5883	327	43	)	)	PUNCT
fnp-5883	327	44	.	.	PUNCT
fnp-5883	328	1	higher	high	ADJ
fnp-5883	328	2	magnification	magnification	NOUN
fnp-5883	328	3	makes	make	VERB
fnp-5883	328	4	the	the	DET
fnp-5883	328	5	ice	ice	NOUN
fnp-5883	328	6	artifacts	artifact	NOUN
fnp-5883	328	7	seen	see	VERB
fnp-5883	328	8	in	in	ADP
fnp-5883	328	9	the	the	DET
fnp-5883	328	10	frozen	frozen	ADJ
fnp-5883	328	11	but	but	CCONJ
fnp-5883	328	12	not	not	PART
fnp-5883	328	13	the	the	DET
fnp-5883	328	14	immersion	immersion	NOUN
fnp-5883	328	15	fixed	fix	VERB
fnp-5883	328	16	tissue	tissue	NOUN
fnp-5883	328	17	more	more	ADV
fnp-5883	328	18	apparent	apparent	ADJ
fnp-5883	328	19	,	,	PUNCT
fnp-5883	328	20	particularly	particularly	ADV
fnp-5883	328	21	in	in	ADP
fnp-5883	328	22	the	the	DET
fnp-5883	328	23	grey	grey	ADJ
fnp-5883	328	24	matter	matter	NOUN
fnp-5883	328	25	(	(	PUNCT
fnp-5883	328	26	c	c	X
fnp-5883	328	27	,	,	PUNCT
fnp-5883	328	28	d	d	NOUN
fnp-5883	328	29	)	)	PUNCT
fnp-5883	328	30	,	,	PUNCT
fnp-5883	328	31	but	but	CCONJ
fnp-5883	328	32	also	also	ADV
fnp-5883	328	33	to	to	ADP
fnp-5883	328	34	a	a	DET
fnp-5883	328	35	lesser	less	ADJ
fnp-5883	328	36	degree	degree	NOUN
fnp-5883	328	37	in	in	ADP
fnp-5883	328	38	the	the	DET
fnp-5883	328	39	white	white	ADJ
fnp-5883	328	40	matter	matter	NOUN
fnp-5883	328	41	(	(	PUNCT
fnp-5883	328	42	e	e	NOUN
fnp-5883	328	43	,	,	PUNCT
fnp-5883	328	44	f	f	NOUN
fnp-5883	328	45	)	)	PUNCT
fnp-5883	328	46	and	and	CCONJ
fnp-5883	328	47	subpial	subpial	ADJ
fnp-5883	328	48	area	area	NOUN
fnp-5883	328	49	(	(	PUNCT
fnp-5883	328	50	g	g	NOUN
fnp-5883	328	51	,	,	PUNCT
fnp-5883	328	52	h	h	NOUN
fnp-5883	328	53	)	)	PUNCT
fnp-5883	328	54	,	,	PUNCT
fnp-5883	328	55	with	with	ADP
fnp-5883	328	56	shrunken	shrunken	ADJ
fnp-5883	328	57	,	,	PUNCT
fnp-5883	328	58	distorted	distort	VERB
fnp-5883	328	59	cells	cell	NOUN
fnp-5883	328	60	and	and	CCONJ
fnp-5883	328	61	large	large	ADJ
fnp-5883	328	62	empty	empty	ADJ
fnp-5883	328	63	spaces	space	NOUN
fnp-5883	328	64	indicative	indicative	ADJ
fnp-5883	328	65	of	of	ADP
fnp-5883	328	66	ice	ice	NOUN
fnp-5883	328	67	crystals	crystal	NOUN
fnp-5883	328	68	.	.	PUNCT
fnp-5883	329	1	scale	scale	NOUN
fnp-5883	329	2	bars	bar	NOUN
fnp-5883	329	3	:	:	PUNCT
fnp-5883	329	4	a	a	DET
fnp-5883	329	5	,	,	PUNCT
fnp-5883	329	6	b	b	NOUN
fnp-5883	329	7	:	:	PUNCT
fnp-5883	329	8	1000	1000	NUM
fnp-5883	329	9	 	 	SPACE
fnp-5883	329	10	μm	μm	INTJ
fnp-5883	329	11	.	.	PUNCT
fnp-5883	330	1	c	c	X
fnp-5883	330	2	–	–	PUNCT
fnp-5883	330	3	h	h	NOUN
fnp-5883	330	4	:	:	PUNCT
fnp-5883	330	5	100	100	NUM
fnp-5883	330	6	 	 	SPACE
fnp-5883	330	7	μm	μm	NOUN
fnp-5883	330	8	.	.	PUNCT
fnp-5883	331	1	summary	summary	NOUN
fnp-5883	331	2	freezing	freezing	NOUN
fnp-5883	331	3	methods	method	NOUN
fnp-5883	331	4	are	be	AUX
fnp-5883	331	5	the	the	DET
fnp-5883	331	6	most	most	ADV
fnp-5883	331	7	common	common	ADJ
fnp-5883	331	8	approach	approach	NOUN
fnp-5883	331	9	for	for	ADP
fnp-5883	331	10	cryopreserving	cryopreserve	VERB
fnp-5883	331	11	relatively	relatively	ADV
fnp-5883	331	12	large	large	ADJ
fnp-5883	331	13	brain	brain	NOUN
fnp-5883	331	14	tissue	tissue	NOUN
fnp-5883	331	15	samples	sample	NOUN
fnp-5883	331	16	.	.	PUNCT
fnp-5883	332	1	the	the	DET
fnp-5883	332	2	efficacy	efficacy	NOUN
fnp-5883	332	3	of	of	ADP
fnp-5883	332	4	freezing	freezing	ADJ
fnp-5883	332	5	methods	method	NOUN
fnp-5883	332	6	varies	vary	VERB
fnp-5883	332	7	considerably	considerably	ADV
fnp-5883	332	8	based	base	VERB
fnp-5883	332	9	on	on	ADP
fnp-5883	332	10	the	the	DET
fnp-5883	332	11	specific	specific	ADJ
fnp-5883	332	12	technique	technique	NOUN
fnp-5883	332	13	used	use	VERB
fnp-5883	332	14	.	.	PUNCT
fnp-5883	333	1	placing	place	VERB
fnp-5883	333	2	large	large	ADJ
fnp-5883	333	3	tissue	tissue	NOUN
fnp-5883	333	4	samples	sample	NOUN
fnp-5883	333	5	directly	directly	ADV
fnp-5883	333	6	in	in	ADP
fnp-5883	333	7	a	a	DET
fnp-5883	333	8	freezer	freezer	NOUN
fnp-5883	333	9	leads	lead	VERB
fnp-5883	333	10	in	in	ADP
fnp-5883	333	11	general	general	ADJ
fnp-5883	333	12	to	to	ADP
fnp-5883	333	13	severe	severe	ADJ
fnp-5883	333	14	ice	ice	NOUN
fnp-5883	333	15	artifacts	artifact	NOUN
fnp-5883	333	16	due	due	ADP
fnp-5883	333	17	to	to	ADP
fnp-5883	333	18	the	the	DET
fnp-5883	333	19	relatively	relatively	ADV
fnp-5883	333	20	slow	slow	ADJ
fnp-5883	333	21	cooling	cool	VERB
fnp-5883	333	22	rates	rate	NOUN
fnp-5883	333	23	.	.	PUNCT
fnp-5883	334	1	on	on	ADP
fnp-5883	334	2	the	the	DET
fnp-5883	334	3	other	other	ADJ
fnp-5883	334	4	hand	hand	NOUN
fnp-5883	334	5	,	,	PUNCT
fnp-5883	334	6	freezing	freeze	VERB
fnp-5883	334	7	brain	brain	NOUN
fnp-5883	334	8	slices	slice	NOUN
fnp-5883	334	9	using	use	VERB
fnp-5883	334	10	either	either	CCONJ
fnp-5883	334	11	pre	pre	ADJ
fnp-5883	334	12	-	-	ADJ
fnp-5883	334	13	cooled	cooled	ADJ
fnp-5883	334	14	metal	metal	NOUN
fnp-5883	334	15	plates	plate	NOUN
fnp-5883	334	16	,	,	PUNCT
fnp-5883	334	17	embedding	embed	VERB
fnp-5883	334	18	in	in	ADP
fnp-5883	334	19	dry	dry	ADJ
fnp-5883	334	20	ice	ice	NOUN
fnp-5883	334	21	,	,	PUNCT
fnp-5883	334	22	immersing	immerse	VERB
fnp-5883	334	23	in	in	ADP
fnp-5883	334	24	cooled	cool	VERB
fnp-5883	334	25	isopentane	isopentane	NOUN
fnp-5883	334	26	,	,	PUNCT
fnp-5883	334	27	or	or	CCONJ
fnp-5883	334	28	freezing	freeze	VERB
fnp-5883	334	29	in	in	ADP
fnp-5883	334	30	liquid	liquid	ADJ
fnp-5883	334	31	nitrogen	nitrogen	NOUN
fnp-5883	334	32	vapor	vapor	NOUN
fnp-5883	334	33	,	,	PUNCT
fnp-5883	334	34	have	have	AUX
fnp-5883	334	35	all	all	PRON
fnp-5883	334	36	been	be	AUX
fnp-5883	334	37	reported	report	VERB
fnp-5883	334	38	to	to	PART
fnp-5883	334	39	achieve	achieve	VERB
fnp-5883	334	40	relatively	relatively	ADV
fnp-5883	334	41	rapid	rapid	ADJ
fnp-5883	334	42	cooling	cooling	NOUN
fnp-5883	334	43	and	and	CCONJ
fnp-5883	334	44	to	to	PART
fnp-5883	334	45	maintain	maintain	VERB
fnp-5883	334	46	morphology	morphology	NOUN
fnp-5883	334	47	with	with	ADP
fnp-5883	334	48	relatively	relatively	ADV
fnp-5883	334	49	few	few	ADJ
fnp-5883	334	50	ice	ice	NOUN
fnp-5883	334	51	artifacts	artifact	NOUN
fnp-5883	334	52	detectable	detectable	ADJ
fnp-5883	334	53	by	by	ADP
fnp-5883	334	54	light	light	ADJ
fnp-5883	334	55	microscopy	microscopy	NOUN
fnp-5883	334	56	.	.	PUNCT
fnp-5883	335	1	cryoprotectant	cryoprotectant	NOUN
fnp-5883	335	2	-	-	PUNCT
fnp-5883	335	3	based	base	VERB
fnp-5883	335	4	cryopreservation	cryopreservation	NOUN
fnp-5883	335	5	preservation	preservation	NOUN
fnp-5883	335	6	process	process	NOUN
fnp-5883	335	7	cryoprotectants	cryoprotectant	NOUN
fnp-5883	335	8	are	be	AUX
fnp-5883	335	9	substances	substance	NOUN
fnp-5883	335	10	that	that	PRON
fnp-5883	335	11	can	can	AUX
fnp-5883	335	12	be	be	AUX
fnp-5883	335	13	added	add	VERB
fnp-5883	335	14	to	to	ADP
fnp-5883	335	15	tissues	tissue	NOUN
fnp-5883	335	16	to	to	PART
fnp-5883	335	17	prevent	prevent	VERB
fnp-5883	335	18	ice	ice	NOUN
fnp-5883	335	19	formation	formation	NOUN
fnp-5883	335	20	during	during	ADP
fnp-5883	335	21	the	the	DET
fnp-5883	335	22	cryopreservation	cryopreservation	NOUN
fnp-5883	335	23	process	process	NOUN
fnp-5883	335	24	.	.	PUNCT
fnp-5883	336	1	the	the	DET
fnp-5883	336	2	most	most	ADV
fnp-5883	336	3	frequently	frequently	ADV
fnp-5883	336	4	used	use	VERB
fnp-5883	336	5	cryoprotectants	cryoprotectant	NOUN
fnp-5883	336	6	reported	report	VERB
fnp-5883	336	7	in	in	ADP
fnp-5883	336	8	the	the	DET
fnp-5883	336	9	studies	study	NOUN
fnp-5883	336	10	we	we	PRON
fnp-5883	336	11	identified	identify	VERB
fnp-5883	336	12	include	include	VERB
fnp-5883	336	13	dimethyl	dimethyl	NOUN
fnp-5883	336	14	sulfoxide	sulfoxide	NOUN
fnp-5883	336	15	(	(	PUNCT
fnp-5883	336	16	dmso	dmso	NOUN
fnp-5883	336	17	)	)	PUNCT
fnp-5883	336	18	,	,	PUNCT
fnp-5883	336	19	glycerol	glycerol	NOUN
fnp-5883	336	20	,	,	PUNCT
fnp-5883	336	21	sucrose	sucrose	NOUN
fnp-5883	336	22	,	,	PUNCT
fnp-5883	336	23	dextran	dextran	NOUN
fnp-5883	336	24	,	,	PUNCT
fnp-5883	336	25	bovine	bovine	NOUN
fnp-5883	336	26	serum	serum	NOUN
fnp-5883	336	27	albumin	albumin	NOUN
fnp-5883	336	28	,	,	PUNCT
fnp-5883	336	29	and	and	CCONJ
fnp-5883	336	30	polyvinylpyrrolidone	polyvinylpyrrolidone	NOUN
fnp-5883	336	31	(	(	PUNCT
fnp-5883	336	32	pvp	pvp	PROPN
fnp-5883	336	33	)	)	PUNCT
fnp-5883	336	34	.	.	PUNCT
fnp-5883	337	1	these	these	DET
fnp-5883	337	2	substances	substance	NOUN
fnp-5883	337	3	act	act	VERB
fnp-5883	337	4	by	by	ADP
fnp-5883	337	5	forming	form	VERB
fnp-5883	337	6	hydrogen	hydrogen	NOUN
fnp-5883	337	7	bonds	bond	NOUN
fnp-5883	337	8	with	with	ADP
fnp-5883	337	9	water	water	NOUN
fnp-5883	337	10	molecules	molecule	NOUN
fnp-5883	337	11	,	,	PUNCT
fnp-5883	337	12	causing	cause	VERB
fnp-5883	337	13	freezing	freezing	NOUN
fnp-5883	337	14	point	point	NOUN
fnp-5883	337	15	depression	depression	NOUN
fnp-5883	337	16	,	,	PUNCT
fnp-5883	337	17	and	and	CCONJ
fnp-5883	337	18	increasing	increase	VERB
fnp-5883	337	19	tissue	tissue	NOUN
fnp-5883	337	20	viscosity	viscosity	NOUN
fnp-5883	337	21	.	.	PUNCT
fnp-5883	338	1	to	to	PART
fnp-5883	338	2	achieve	achieve	VERB
fnp-5883	338	3	complete	complete	ADJ
fnp-5883	338	4	vitrification	vitrification	NOUN
fnp-5883	338	5	and	and	CCONJ
fnp-5883	338	6	prevent	prevent	VERB
fnp-5883	338	7	ice	ice	NOUN
fnp-5883	338	8	formation	formation	NOUN
fnp-5883	338	9	entirely	entirely	ADV
fnp-5883	338	10	,	,	PUNCT
fnp-5883	338	11	a	a	DET
fnp-5883	338	12	high	high	ADJ
fnp-5883	338	13	concentration	concentration	NOUN
fnp-5883	338	14	of	of	ADP
fnp-5883	338	15	cryoprotectants	cryoprotectant	NOUN
fnp-5883	338	16	is	be	AUX
fnp-5883	338	17	needed	need	VERB
fnp-5883	338	18	.	.	PUNCT
fnp-5883	339	1	for	for	ADP
fnp-5883	339	2	example	example	NOUN
fnp-5883	339	3	,	,	PUNCT
fnp-5883	339	4	a	a	DET
fnp-5883	339	5	solution	solution	NOUN
fnp-5883	339	6	containing	contain	VERB
fnp-5883	339	7	61	61	NUM
fnp-5883	339	8	 	 	SPACE
fnp-5883	339	9	%	%	NOUN
fnp-5883	339	10	w	w	PROPN
fnp-5883	339	11	/	/	SYM
fnp-5883	339	12	v	v	NOUN
fnp-5883	339	13	ethylene	ethylene	NOUN
fnp-5883	339	14	glycol	glycol	NOUN
fnp-5883	339	15	has	have	AUX
fnp-5883	339	16	been	be	AUX
fnp-5883	339	17	used	use	VERB
fnp-5883	339	18	to	to	PART
fnp-5883	339	19	vitrify	vitrify	VERB
fnp-5883	339	20	mouse	mouse	NOUN
fnp-5883	339	21	brain	brain	NOUN
fnp-5883	339	22	slices	slice	NOUN
fnp-5883	339	23	alongside	alongside	ADP
fnp-5883	339	24	cooling	cool	VERB
fnp-5883	339	25	in	in	ADP
fnp-5883	339	26	liquid	liquid	ADJ
fnp-5883	339	27	nitrogen	nitrogen	NOUN
fnp-5883	339	28	(	(	PUNCT
fnp-5883	339	29	german	german	NOUN
fnp-5883	339	30	and	and	CCONJ
fnp-5883	339	31	akdaş	akdaş	NOUN
fnp-5883	339	32	,	,	PUNCT
fnp-5883	339	33	2024	2024	NUM
fnp-5883	339	34	)	)	PUNCT
fnp-5883	339	35	.	.	PUNCT
fnp-5883	340	1	lower	low	ADJ
fnp-5883	340	2	concentrations	concentration	NOUN
fnp-5883	340	3	of	of	ADP
fnp-5883	340	4	cryoprotectant	cryoprotectant	NOUN
fnp-5883	340	5	have	have	AUX
fnp-5883	340	6	been	be	AUX
fnp-5883	340	7	used	use	VERB
fnp-5883	340	8	in	in	ADP
fnp-5883	340	9	various	various	ADJ
fnp-5883	340	10	studies	study	NOUN
fnp-5883	340	11	but	but	CCONJ
fnp-5883	340	12	are	be	AUX
fnp-5883	340	13	not	not	PART
fnp-5883	340	14	likely	likely	ADJ
fnp-5883	340	15	to	to	PART
fnp-5883	340	16	achieve	achieve	VERB
fnp-5883	340	17	complete	complete	ADJ
fnp-5883	340	18	vitrification	vitrification	NOUN
fnp-5883	340	19	.	.	PUNCT
fnp-5883	341	1	notably	notably	ADV
fnp-5883	341	2	,	,	PUNCT
fnp-5883	341	3	cryoprotectants	cryoprotectant	NOUN
fnp-5883	341	4	can	can	AUX
fnp-5883	341	5	also	also	ADV
fnp-5883	341	6	cause	cause	VERB
fnp-5883	341	7	structural	structural	ADJ
fnp-5883	341	8	damage	damage	NOUN
fnp-5883	341	9	.	.	PUNCT
fnp-5883	342	1	for	for	ADP
fnp-5883	342	2	example	example	NOUN
fnp-5883	342	3	,	,	PUNCT
fnp-5883	342	4	dmso	dmso	NOUN
fnp-5883	342	5	and	and	CCONJ
fnp-5883	342	6	glycerol	glycerol	NOUN
fnp-5883	342	7	have	have	AUX
fnp-5883	342	8	both	both	PRON
fnp-5883	342	9	been	be	AUX
fnp-5883	342	10	reported	report	VERB
fnp-5883	342	11	to	to	PART
fnp-5883	342	12	cause	cause	VERB
fnp-5883	342	13	ultrastructural	ultrastructural	ADJ
fnp-5883	342	14	alterations	alteration	NOUN
fnp-5883	342	15	to	to	ADP
fnp-5883	342	16	the	the	DET
fnp-5883	342	17	cell	cell	NOUN
fnp-5883	342	18	membrane	membrane	NOUN
fnp-5883	342	19	,	,	PUNCT
fnp-5883	342	20	with	with	ADP
fnp-5883	342	21	dmso	dmso	NOUN
fnp-5883	342	22	being	be	AUX
fnp-5883	342	23	more	more	ADV
fnp-5883	342	24	damaging	damaging	ADJ
fnp-5883	342	25	than	than	ADP
fnp-5883	342	26	glycerol	glycerol	NOUN
fnp-5883	342	27	(	(	PUNCT
fnp-5883	342	28	mcintyre	mcintyre	PROPN
fnp-5883	342	29	et	et	PROPN
fnp-5883	342	30	al	al	PROPN
fnp-5883	342	31	.	.	PROPN
fnp-5883	342	32	,	,	PUNCT
fnp-5883	342	33	1974	1974	NUM
fnp-5883	342	34	)	)	PUNCT
fnp-5883	342	35	.	.	PUNCT
fnp-5883	343	1	furthermore	furthermore	ADV
fnp-5883	343	2	,	,	PUNCT
fnp-5883	343	3	cell	cell	NOUN
fnp-5883	343	4	organelles	organelle	NOUN
fnp-5883	343	5	can	can	AUX
fnp-5883	343	6	be	be	AUX
fnp-5883	343	7	disrupted	disrupt	VERB
fnp-5883	343	8	and	and	CCONJ
fnp-5883	343	9	myelin	myelin	NOUN
fnp-5883	343	10	-	-	PUNCT
fnp-5883	343	11	like	like	ADJ
fnp-5883	343	12	figures	figure	NOUN
fnp-5883	343	13	can	can	AUX
fnp-5883	343	14	occur	occur	VERB
fnp-5883	343	15	within	within	ADP
fnp-5883	343	16	the	the	DET
fnp-5883	343	17	cytoplasm	cytoplasm	NOUN
fnp-5883	343	18	.	.	PUNCT
fnp-5883	344	1	cryoprotectant	cryoprotectant	NOUN
fnp-5883	344	2	immersion	immersion	NOUN
fnp-5883	344	3	several	several	ADJ
fnp-5883	344	4	studies	study	NOUN
fnp-5883	344	5	that	that	PRON
fnp-5883	344	6	we	we	PRON
fnp-5883	344	7	identified	identify	VERB
fnp-5883	344	8	reported	report	VERB
fnp-5883	344	9	immersion	immersion	NOUN
fnp-5883	344	10	of	of	ADP
fnp-5883	344	11	cryoprotectants	cryoprotectant	NOUN
fnp-5883	344	12	into	into	ADP
fnp-5883	344	13	brain	brain	NOUN
fnp-5883	344	14	tissue	tissue	NOUN
fnp-5883	344	15	prior	prior	ADV
fnp-5883	344	16	to	to	ADP
fnp-5883	344	17	cryopreservation	cryopreservation	NOUN
fnp-5883	344	18	(	(	PUNCT
fnp-5883	344	19	table	table	NOUN
fnp-5883	344	20	 	 	SPACE
fnp-5883	344	21	4	4	NUM
fnp-5883	344	22	;	;	PUNCT
fnp-5883	344	23	supplementary	supplementary	ADJ
fnp-5883	344	24	file	file	NOUN
fnp-5883	344	25	 	 	SPACE
fnp-5883	344	26	4	4	NUM
fnp-5883	344	27	)	)	PUNCT
fnp-5883	344	28	.	.	PUNCT
fnp-5883	345	1	among	among	ADP
fnp-5883	345	2	these	these	PRON
fnp-5883	345	3	,	,	PUNCT
fnp-5883	345	4	multiple	multiple	ADJ
fnp-5883	345	5	studies	study	NOUN
fnp-5883	345	6	found	find	VERB
fnp-5883	345	7	that	that	SCONJ
fnp-5883	345	8	areas	area	NOUN
fnp-5883	345	9	with	with	ADP
fnp-5883	345	10	less	less	ADJ
fnp-5883	345	11	take	take	VERB
fnp-5883	345	12	-	-	PUNCT
fnp-5883	345	13	up	up	NOUN
fnp-5883	345	14	of	of	ADP
fnp-5883	345	15	cryoprotectant	cryoprotectant	NOUN
fnp-5883	345	16	tend	tend	VERB
fnp-5883	345	17	to	to	PART
fnp-5883	345	18	have	have	VERB
fnp-5883	345	19	more	more	ADJ
fnp-5883	345	20	freezing	freezing	ADJ
fnp-5883	345	21	artifacts	artifact	NOUN
fnp-5883	345	22	,	,	PUNCT
fnp-5883	345	23	but	but	CCONJ
fnp-5883	345	24	that	that	SCONJ
fnp-5883	345	25	in	in	ADP
fnp-5883	345	26	areas	area	NOUN
fnp-5883	345	27	where	where	SCONJ
fnp-5883	345	28	an	an	DET
fnp-5883	345	29	adequate	adequate	ADJ
fnp-5883	345	30	concentration	concentration	NOUN
fnp-5883	345	31	of	of	ADP
fnp-5883	345	32	cryoprotectant	cryoprotectant	NOUN
fnp-5883	345	33	was	be	AUX
fnp-5883	345	34	successfully	successfully	ADV
fnp-5883	345	35	taken	take	VERB
fnp-5883	345	36	up	up	ADP
fnp-5883	345	37	,	,	PUNCT
fnp-5883	345	38	cells	cell	NOUN
fnp-5883	345	39	are	be	AUX
fnp-5883	345	40	undamaged	undamaged	ADJ
fnp-5883	345	41	(	(	PUNCT
fnp-5883	345	42	seilhean	seilhean	PROPN
fnp-5883	345	43	et	et	PROPN
fnp-5883	345	44	al	al	PROPN
fnp-5883	345	45	.	.	PROPN
fnp-5883	345	46	,	,	PUNCT
fnp-5883	345	47	1996	1996	NUM
fnp-5883	345	48	;	;	PUNCT
fnp-5883	345	49	pichugin	pichugin	NOUN
fnp-5883	345	50	et	et	PROPN
fnp-5883	345	51	al	al	PROPN
fnp-5883	345	52	.	.	PROPN
fnp-5883	345	53	,	,	PUNCT
fnp-5883	345	54	2006	2006	NUM
fnp-5883	345	55	)	)	PUNCT
fnp-5883	345	56	.	.	PUNCT
fnp-5883	346	1	another	another	DET
fnp-5883	346	2	study	study	NOUN
fnp-5883	346	3	demonstrated	demonstrate	VERB
fnp-5883	346	4	a	a	DET
fnp-5883	346	5	potential	potential	ADJ
fnp-5883	346	6	issue	issue	NOUN
fnp-5883	346	7	with	with	ADP
fnp-5883	346	8	cryoprotectant	cryoprotectant	NOUN
fnp-5883	346	9	immersion	immersion	NOUN
fnp-5883	346	10	,	,	PUNCT
fnp-5883	346	11	as	as	SCONJ
fnp-5883	346	12	cellular	cellular	ADJ
fnp-5883	346	13	necrosis	necrosis	NOUN
fnp-5883	346	14	occurred	occur	VERB
fnp-5883	346	15	during	during	ADP
fnp-5883	346	16	the	the	DET
fnp-5883	346	17	time	time	NOUN
fnp-5883	346	18	necessary	necessary	ADJ
fnp-5883	346	19	for	for	SCONJ
fnp-5883	346	20	the	the	DET
fnp-5883	346	21	immersion	immersion	NOUN
fnp-5883	346	22	procedure	procedure	NOUN
fnp-5883	346	23	to	to	PART
fnp-5883	346	24	be	be	AUX
fnp-5883	346	25	completed	complete	VERB
fnp-5883	346	26	(	(	PUNCT
fnp-5883	346	27	german	german	ADJ
fnp-5883	346	28	and	and	CCONJ
fnp-5883	346	29	akdaş	akdaş	NOUN
fnp-5883	346	30	,	,	PUNCT
fnp-5883	346	31	2024	2024	NUM
fnp-5883	346	32	)	)	PUNCT
fnp-5883	346	33	.	.	PUNCT
fnp-5883	347	1	sample	sample	NOUN
fnp-5883	347	2	type	type	NOUN
fnp-5883	347	3	(	(	PUNCT
fnp-5883	347	4	study	study	NOUN
fnp-5883	347	5	)	)	PUNCT
fnp-5883	347	6	cooling	cool	VERB
fnp-5883	347	7	method	method	NOUN
fnp-5883	347	8	visualization	visualization	NOUN
fnp-5883	347	9	method	method	NOUN
fnp-5883	347	10	key	key	ADJ
fnp-5883	347	11	findings	finding	NOUN
fnp-5883	347	12	mouse	mouse	NOUN
fnp-5883	347	13	sciatic	sciatic	ADJ
fnp-5883	347	14	nerves	nerve	NOUN
fnp-5883	347	15	(	(	PUNCT
fnp-5883	347	16	kirschner	kirschner	PROPN
fnp-5883	347	17	et	et	PROPN
fnp-5883	347	18	al	al	PROPN
fnp-5883	347	19	.	.	PROPN
fnp-5883	347	20	,	,	PUNCT
fnp-5883	347	21	1979	1979	NUM
fnp-5883	347	22	)	)	PUNCT
fnp-5883	347	23	immersion	immersion	NOUN
fnp-5883	347	24	in	in	ADP
fnp-5883	347	25	20	20	NUM
fnp-5883	347	26	 	 	SPACE
fnp-5883	347	27	%	%	NOUN
fnp-5883	347	28	dmso	dmso	NOUN
fnp-5883	347	29	,	,	PUNCT
fnp-5883	347	30	frozen	freeze	VERB
fnp-5883	347	31	in	in	ADP
fnp-5883	347	32	a	a	DET
fnp-5883	347	33	freon	freon	NOUN
fnp-5883	347	34	22	22	NUM
fnp-5883	347	35	slush	slush	NOUN
fnp-5883	347	36	cooled	cool	VERB
fnp-5883	347	37	with	with	ADP
fnp-5883	347	38	liquid	liquid	ADJ
fnp-5883	347	39	nitrogen	nitrogen	NOUN
fnp-5883	347	40	x	x	NOUN
fnp-5883	347	41	-	-	NOUN
fnp-5883	347	42	ray	ray	NOUN
fnp-5883	347	43	diffraction	diffraction	NOUN
fnp-5883	347	44	,	,	PUNCT
fnp-5883	347	45	freeze	freeze	NOUN
fnp-5883	347	46	-	-	PUNCT
fnp-5883	347	47	fracture	fracture	NOUN
fnp-5883	347	48	electron	electron	NOUN
fnp-5883	347	49	microscopy	microscopy	NOUN
fnp-5883	347	50	well	well	ADV
fnp-5883	347	51	-	-	PUNCT
fnp-5883	347	52	ordered	order	VERB
fnp-5883	347	53	myelin	myelin	NOUN
fnp-5883	347	54	membranes	membrane	NOUN
fnp-5883	347	55	,	,	PUNCT
fnp-5883	347	56	slightly	slightly	ADV
fnp-5883	347	57	larger	large	ADJ
fnp-5883	347	58	myelin	myelin	NOUN
fnp-5883	347	59	sheaths	sheath	NOUN
fnp-5883	347	60	compared	compare	VERB
fnp-5883	347	61	to	to	PART
fnp-5883	347	62	control	control	VERB
fnp-5883	347	63	tissue	tissue	NOUN
fnp-5883	347	64	rat	rat	NOUN
fnp-5883	347	65	embryonic	embryonic	ADJ
fnp-5883	347	66	neural	neural	ADJ
fnp-5883	347	67	structures	structure	NOUN
fnp-5883	347	68	(	(	PUNCT
fnp-5883	347	69	das	das	PROPN
fnp-5883	347	70	et	et	PROPN
fnp-5883	347	71	al	al	PROPN
fnp-5883	347	72	.	.	PROPN
fnp-5883	347	73	,	,	PUNCT
fnp-5883	347	74	1983	1983	NUM
fnp-5883	347	75	)	)	PUNCT
fnp-5883	347	76	immersion	immersion	NOUN
fnp-5883	347	77	in	in	ADP
fnp-5883	347	78	10	10	NUM
fnp-5883	347	79	 	 	SPACE
fnp-5883	347	80	%	%	NOUN
fnp-5883	347	81	dmso	dmso	NOUN
fnp-5883	347	82	,	,	PUNCT
fnp-5883	347	83	frozen	freeze	VERB
fnp-5883	347	84	in	in	ADP
fnp-5883	347	85	dry	dry	ADJ
fnp-5883	347	86	ice	ice	NOUN
fnp-5883	347	87	vapors	vapor	NOUN
fnp-5883	347	88	light	light	ADJ
fnp-5883	347	89	microscopy	microscopy	NOUN
fnp-5883	347	90	,	,	PUNCT
fnp-5883	347	91	cresyl	cresyl	ADJ
fnp-5883	347	92	violet	violet	ADJ
fnp-5883	347	93	staining	stain	VERB
fnp-5883	347	94	tissue	tissue	NOUN
fnp-5883	347	95	and	and	CCONJ
fnp-5883	347	96	cells	cell	NOUN
fnp-5883	347	97	appear	appear	VERB
fnp-5883	347	98	histologically	histologically	ADV
fnp-5883	347	99	normal	normal	ADJ
fnp-5883	347	100	human	human	NOUN
fnp-5883	347	101	or	or	CCONJ
fnp-5883	347	102	mouse	mouse	NOUN
fnp-5883	347	103	fetal	fetal	ADJ
fnp-5883	347	104	cns	cns	NOUN
fnp-5883	347	105	,	,	PUNCT
fnp-5883	347	106	about	about	ADV
fnp-5883	347	107	1	1	NUM
fnp-5883	347	108	 	 	SPACE
fnp-5883	347	109	mm3	mm3	NOUN
fnp-5883	347	110	(	(	PUNCT
fnp-5883	347	111	seilhean	seilhean	VERB
fnp-5883	347	112	et	et	PROPN
fnp-5883	347	113	al	al	PROPN
fnp-5883	347	114	.	.	PROPN
fnp-5883	347	115	,	,	PUNCT
fnp-5883	347	116	1996	1996	NUM
fnp-5883	347	117	)	)	PUNCT
fnp-5883	348	1	immersion	immersion	NOUN
fnp-5883	348	2	in	in	ADP
fnp-5883	348	3	10	10	NUM
fnp-5883	348	4	 	 	SPACE
fnp-5883	348	5	%	%	NOUN
fnp-5883	348	6	dmso	dmso	NOUN
fnp-5883	348	7	at	at	ADP
fnp-5883	348	8	4	4	NUM
fnp-5883	348	9	 	 	SPACE
fnp-5883	348	10	°	°	NOUN
fnp-5883	348	11	c	c	NOUN
fnp-5883	348	12	,	,	PUNCT
fnp-5883	348	13	kept	keep	VERB
fnp-5883	348	14	at	at	ADP
fnp-5883	348	15	‑80	‑80	NOUN
fnp-5883	348	16	 	 	SPACE
fnp-5883	348	17	°	°	NOUN
fnp-5883	348	18	c	c	NOUN
fnp-5883	348	19	overnight	overnight	ADV
fnp-5883	348	20	,	,	PUNCT
fnp-5883	348	21	then	then	ADV
fnp-5883	348	22	frozen	freeze	VERB
fnp-5883	348	23	in	in	ADP
fnp-5883	348	24	liquid	liquid	ADJ
fnp-5883	348	25	nitrogen	nitrogen	NOUN
fnp-5883	348	26	rewarming	rewarming	NOUN
fnp-5883	348	27	at	at	ADP
fnp-5883	348	28	37	37	NUM
fnp-5883	348	29	 	 	SPACE
fnp-5883	348	30	°	°	NOUN
fnp-5883	348	31	c	c	NOUN
fnp-5883	348	32	,	,	PUNCT
fnp-5883	348	33	then	then	ADV
fnp-5883	348	34	electron	electron	NOUN
fnp-5883	348	35	microscopy	microscopy	NOUN
fnp-5883	348	36	cells	cell	NOUN
fnp-5883	348	37	in	in	ADP
fnp-5883	348	38	the	the	DET
fnp-5883	348	39	periphery	periphery	NOUN
fnp-5883	348	40	of	of	ADP
fnp-5883	348	41	the	the	DET
fnp-5883	348	42	sample	sample	NOUN
fnp-5883	348	43	are	be	AUX
fnp-5883	348	44	intact	intact	ADJ
fnp-5883	348	45	,	,	PUNCT
fnp-5883	348	46	but	but	CCONJ
fnp-5883	348	47	disrupted	disrupt	VERB
fnp-5883	348	48	membranes	membrane	NOUN
fnp-5883	348	49	in	in	ADP
fnp-5883	348	50	center	center	ADJ
fnp-5883	348	51	areas	area	NOUN
fnp-5883	348	52	,	,	PUNCT
fnp-5883	348	53	possibly	possibly	ADV
fnp-5883	348	54	due	due	ADP
fnp-5883	348	55	to	to	ADP
fnp-5883	348	56	ice	ice	NOUN
fnp-5883	348	57	damage	damage	NOUN
fnp-5883	348	58	475	475	NUM
fnp-5883	348	59	 	 	SPACE
fnp-5883	348	60	µm	µm	ADP
fnp-5883	348	61	thick	thick	ADJ
fnp-5883	348	62	rat	rat	NOUN
fnp-5883	348	63	hippocampal	hippocampal	ADJ
fnp-5883	348	64	slices	slice	NOUN
fnp-5883	348	65	(	(	PUNCT
fnp-5883	348	66	pichugin	pichugin	NOUN
fnp-5883	348	67	et	et	PROPN
fnp-5883	348	68	al	al	PROPN
fnp-5883	348	69	.	.	PROPN
fnp-5883	348	70	,	,	PUNCT
fnp-5883	348	71	2006	2006	NUM
fnp-5883	348	72	)	)	PUNCT
fnp-5883	348	73	immersion	immersion	NOUN
fnp-5883	348	74	in	in	ADP
fnp-5883	348	75	vm3	vm3	NOUN
fnp-5883	348	76	vitrification	vitrification	NOUN
fnp-5883	348	77	solution	solution	NOUN
fnp-5883	348	78	,	,	PUNCT
fnp-5883	348	79	exposed	expose	VERB
fnp-5883	348	80	to	to	ADP
fnp-5883	348	81	aluminum	aluminum	NOUN
fnp-5883	348	82	block	block	NOUN
fnp-5883	348	83	cooled	cool	VERB
fnp-5883	348	84	to	to	ADP
fnp-5883	348	85	‑130	‑130	NOUN
fnp-5883	348	86	 	 	SPACE
fnp-5883	348	87	°	°	ADP
fnp-5883	348	88	c	c	NOUN
fnp-5883	348	89	rewarming	rewarming	NOUN
fnp-5883	348	90	to	to	ADP
fnp-5883	348	91	35	35	NUM
fnp-5883	348	92	 	 	SPACE
fnp-5883	348	93	°	°	NOUN
fnp-5883	348	94	c	c	NOUN
fnp-5883	348	95	,	,	PUNCT
fnp-5883	348	96	fixation	fixation	NOUN
fnp-5883	348	97	,	,	PUNCT
fnp-5883	348	98	then	then	ADV
fnp-5883	348	99	electron	electron	NOUN
fnp-5883	348	100	microscopy	microscopy	NOUN
fnp-5883	348	101	with	with	ADP
fnp-5883	348	102	adequate	adequate	ADJ
fnp-5883	348	103	uptake	uptake	NOUN
fnp-5883	348	104	of	of	ADP
fnp-5883	348	105	cryoprotectant	cryoprotectant	NOUN
fnp-5883	348	106	,	,	PUNCT
fnp-5883	348	107	ultrastructure	ultrastructure	NOUN
fnp-5883	348	108	is	be	AUX
fnp-5883	348	109	high	high	ADJ
fnp-5883	348	110	-	-	PUNCT
fnp-5883	348	111	quality	quality	NOUN
fnp-5883	348	112	,	,	PUNCT
fnp-5883	348	113	effectively	effectively	ADV
fnp-5883	348	114	equivalent	equivalent	ADJ
fnp-5883	348	115	to	to	ADP
fnp-5883	348	116	that	that	PRON
fnp-5883	348	117	of	of	ADP
fnp-5883	348	118	controls	control	NOUN
fnp-5883	348	119	400	400	NUM
fnp-5883	348	120	 	 	SPACE
fnp-5883	348	121	μm	μm	NUM
fnp-5883	348	122	thick	thick	ADJ
fnp-5883	348	123	mouse	mouse	PROPN
fnp-5883	348	124	cortico	cortico	NOUN
fnp-5883	348	125	-	-	PUNCT
fnp-5883	348	126	hippocampal	hippocampal	ADJ
fnp-5883	348	127	slices	slice	NOUN
fnp-5883	348	128	(	(	PUNCT
fnp-5883	348	129	german	german	NOUN
fnp-5883	348	130	and	and	CCONJ
fnp-5883	348	131	akdaş	akdaş	NOUN
fnp-5883	348	132	,	,	PUNCT
fnp-5883	348	133	2024	2024	NUM
fnp-5883	348	134	)	)	PUNCT
fnp-5883	348	135	stepwise	stepwise	ADJ
fnp-5883	348	136	immersion	immersion	NOUN
fnp-5883	348	137	in	in	ADP
fnp-5883	348	138	ethylene	ethylene	NOUN
fnp-5883	348	139	glycol	glycol	NOUN
fnp-5883	348	140	,	,	PUNCT
fnp-5883	348	141	up	up	ADP
fnp-5883	348	142	to	to	PART
fnp-5883	348	143	61	61	NUM
fnp-5883	348	144	 	 	SPACE
fnp-5883	348	145	%	%	NOUN
fnp-5883	348	146	w	w	PROPN
fnp-5883	348	147	/	/	SYM
fnp-5883	348	148	v	v	NOUN
fnp-5883	348	149	ethylene	ethylene	NOUN
fnp-5883	348	150	glycol	glycol	NOUN
fnp-5883	348	151	,	,	PUNCT
fnp-5883	348	152	then	then	ADV
fnp-5883	348	153	transferred	transfer	VERB
fnp-5883	348	154	into	into	ADP
fnp-5883	348	155	liquid	liquid	ADJ
fnp-5883	348	156	nitrogen	nitrogen	NOUN
fnp-5883	348	157	stepwise	stepwise	NOUN
fnp-5883	348	158	removal	removal	NOUN
fnp-5883	348	159	of	of	ADP
fnp-5883	348	160	cryoprotectant	cryoprotectant	NOUN
fnp-5883	348	161	,	,	PUNCT
fnp-5883	348	162	electrophysiology	electrophysiology	NOUN
fnp-5883	348	163	,	,	PUNCT
fnp-5883	348	164	fixation	fixation	NOUN
fnp-5883	348	165	,	,	PUNCT
fnp-5883	348	166	then	then	ADV
fnp-5883	348	167	microscopy	microscopy	VERB
fnp-5883	348	168	light	light	NOUN
fnp-5883	348	169	and	and	CCONJ
fnp-5883	348	170	electron	electron	NOUN
fnp-5883	348	171	microscopy	microscopy	NOUN
fnp-5883	348	172	shows	show	VERB
fnp-5883	348	173	cell	cell	NOUN
fnp-5883	348	174	swelling	swelling	NOUN
fnp-5883	348	175	,	,	PUNCT
fnp-5883	348	176	vacuolization	vacuolization	NOUN
fnp-5883	348	177	,	,	PUNCT
fnp-5883	348	178	and	and	CCONJ
fnp-5883	348	179	cell	cell	NOUN
fnp-5883	348	180	membrane	membrane	NOUN
fnp-5883	348	181	damage	damage	NOUN
fnp-5883	348	182	after	after	ADP
fnp-5883	348	183	electrophysiological	electrophysiological	ADJ
fnp-5883	348	184	recordings	recording	NOUN
fnp-5883	348	185	table	table	NOUN
fnp-5883	348	186	 	 	SPACE
fnp-5883	348	187	4	4	NUM
fnp-5883	348	188	.	.	PUNCT
fnp-5883	349	1	reported	report	VERB
fnp-5883	349	2	histologic	histologic	ADJ
fnp-5883	349	3	effects	effect	NOUN
fnp-5883	349	4	in	in	ADP
fnp-5883	349	5	representative	representative	ADJ
fnp-5883	349	6	observations	observation	NOUN
fnp-5883	349	7	from	from	ADP
fnp-5883	349	8	studies	study	NOUN
fnp-5883	349	9	using	use	VERB
fnp-5883	349	10	cryopreservation	cryopreservation	NOUN
fnp-5883	349	11	with	with	ADP
fnp-5883	349	12	cryoprotectant	cryoprotectant	NOUN
fnp-5883	349	13	immersion	immersion	NOUN
fnp-5883	349	14	.	.	PUNCT
fnp-5883	350	1	dmso	dmso	NOUN
fnp-5883	350	2	:	:	PUNCT
fnp-5883	350	3	dimethyl	dimethyl	NOUN
fnp-5883	350	4	sulfoxide	sulfoxide	NOUN
fnp-5883	350	5	;	;	PUNCT
fnp-5883	350	6	cns	cns	NOUN
fnp-5883	350	7	:	:	PUNCT
fnp-5883	350	8	central	central	ADJ
fnp-5883	350	9	nervous	nervous	ADJ
fnp-5883	350	10	system	system	NOUN
fnp-5883	350	11	;	;	PUNCT
fnp-5883	350	12	vm3	vm3	NOUN
fnp-5883	350	13	:	:	PUNCT
fnp-5883	350	14	vitrification	vitrification	NOUN
fnp-5883	350	15	solution	solution	NOUN
fnp-5883	350	16	.	.	PUNCT
fnp-5883	351	1	cryoprotectant	cryoprotectant	NOUN
fnp-5883	351	2	perfusion	perfusion	NOUN
fnp-5883	351	3	perfusion	perfusion	NOUN
fnp-5883	351	4	is	be	AUX
fnp-5883	351	5	an	an	DET
fnp-5883	351	6	alternative	alternative	ADJ
fnp-5883	351	7	method	method	NOUN
fnp-5883	351	8	for	for	ADP
fnp-5883	351	9	delivering	deliver	VERB
fnp-5883	351	10	cryoprotectants	cryoprotectant	NOUN
fnp-5883	351	11	through	through	ADP
fnp-5883	351	12	the	the	DET
fnp-5883	351	13	cerebrovascular	cerebrovascular	ADJ
fnp-5883	351	14	system	system	NOUN
fnp-5883	351	15	that	that	PRON
fnp-5883	351	16	can	can	AUX
fnp-5883	351	17	potentially	potentially	ADV
fnp-5883	351	18	achieve	achieve	VERB
fnp-5883	351	19	more	more	ADJ
fnp-5883	351	20	uniform	uniform	ADJ
fnp-5883	351	21	distribution	distribution	NOUN
fnp-5883	351	22	.	.	PUNCT
fnp-5883	352	1	several	several	ADJ
fnp-5883	352	2	studies	study	NOUN
fnp-5883	352	3	that	that	PRON
fnp-5883	352	4	we	we	PRON
fnp-5883	352	5	identified	identify	VERB
fnp-5883	352	6	used	use	VERB
fnp-5883	352	7	perfusion	perfusion	NOUN
fnp-5883	352	8	of	of	ADP
fnp-5883	352	9	cryoprotectants	cryoprotectant	NOUN
fnp-5883	352	10	through	through	ADP
fnp-5883	352	11	the	the	DET
fnp-5883	352	12	blood	blood	NOUN
fnp-5883	352	13	vessels	vessel	NOUN
fnp-5883	352	14	of	of	ADP
fnp-5883	352	15	the	the	DET
fnp-5883	352	16	brain	brain	NOUN
fnp-5883	352	17	in	in	ADP
fnp-5883	352	18	laboratory	laboratory	NOUN
fnp-5883	352	19	animals	animal	NOUN
fnp-5883	352	20	(	(	PUNCT
fnp-5883	352	21	table	table	NOUN
fnp-5883	352	22	 	 	SPACE
fnp-5883	352	23	5	5	NUM
fnp-5883	352	24	;	;	PUNCT
fnp-5883	352	25	supplementary	supplementary	ADJ
fnp-5883	352	26	file	file	NOUN
fnp-5883	352	27	 	 	SPACE
fnp-5883	352	28	4	4	NUM
fnp-5883	352	29	)	)	PUNCT
fnp-5883	352	30	.	.	PUNCT
fnp-5883	353	1	one	one	NUM
fnp-5883	353	2	study	study	NOUN
fnp-5883	353	3	tested	test	VERB
fnp-5883	353	4	perfusion	perfusion	NOUN
fnp-5883	353	5	of	of	ADP
fnp-5883	353	6	rat	rat	NOUN
fnp-5883	353	7	brains	brain	NOUN
fnp-5883	353	8	with	with	ADP
fnp-5883	353	9	either	either	CCONJ
fnp-5883	353	10	a	a	DET
fnp-5883	353	11	vitrification	vitrification	NOUN
fnp-5883	353	12	solution	solution	NOUN
fnp-5883	353	13	called	call	VERB
fnp-5883	353	14	vm1	vm1	PROPN
fnp-5883	353	15	or	or	CCONJ
fnp-5883	353	16	a	a	DET
fnp-5883	353	17	solution	solution	NOUN
fnp-5883	353	18	containing	contain	VERB
fnp-5883	353	19	13	13	NUM
fnp-5883	353	20	 	 	SPACE
fnp-5883	353	21	%	%	NOUN
fnp-5883	353	22	dmso	dmso	NOUN
fnp-5883	353	23	and	and	CCONJ
fnp-5883	353	24	13	13	NUM
fnp-5883	353	25	 	 	SPACE
fnp-5883	353	26	%	%	NOUN
fnp-5883	353	27	glycerol	glycerol	NOUN
fnp-5883	353	28	(	(	PUNCT
fnp-5883	353	29	canatelli	canatelli	NOUN
fnp-5883	353	30	-	-	PUNCT
fnp-5883	353	31	mallat	mallat	PROPN
fnp-5883	353	32	et	et	PROPN
fnp-5883	353	33	al	al	PROPN
fnp-5883	353	34	.	.	PROPN
fnp-5883	353	35	,	,	PUNCT
fnp-5883	353	36	2020	2020	NUM
fnp-5883	353	37	)	)	PUNCT
fnp-5883	353	38	.	.	PUNCT
fnp-5883	354	1	while	while	SCONJ
fnp-5883	354	2	no	no	DET
fnp-5883	354	3	ice	ice	NOUN
fnp-5883	354	4	formation	formation	NOUN
fnp-5883	354	5	was	be	AUX
fnp-5883	354	6	observed	observe	VERB
fnp-5883	354	7	in	in	ADP
fnp-5883	354	8	either	either	DET
fnp-5883	354	9	group	group	NOUN
fnp-5883	354	10	on	on	ADP
fnp-5883	354	11	light	light	ADJ
fnp-5883	354	12	microscopy	microscopy	NOUN
fnp-5883	354	13	,	,	PUNCT
fnp-5883	354	14	significant	significant	ADJ
fnp-5883	354	15	shrinkage	shrinkage	NOUN
fnp-5883	354	16	of	of	ADP
fnp-5883	354	17	neurons	neuron	NOUN
fnp-5883	354	18	occurred	occur	VERB
fnp-5883	354	19	.	.	PUNCT
fnp-5883	355	1	another	another	DET
fnp-5883	355	2	study	study	NOUN
fnp-5883	355	3	used	use	VERB
fnp-5883	355	4	perfusion	perfusion	NOUN
fnp-5883	355	5	of	of	ADP
fnp-5883	355	6	mouse	mouse	NOUN
fnp-5883	355	7	brains	brain	NOUN
fnp-5883	355	8	with	with	ADP
fnp-5883	355	9	30	30	NUM
fnp-5883	355	10	 	 	SPACE
fnp-5883	355	11	%	%	NOUN
fnp-5883	355	12	sucrose	sucrose	NOUN
fnp-5883	355	13	for	for	ADP
fnp-5883	355	14	15	15	NUM
fnp-5883	355	15	minutes	minute	NOUN
fnp-5883	355	16	,	,	PUNCT
fnp-5883	355	17	followed	follow	VERB
fnp-5883	355	18	by	by	ADP
fnp-5883	355	19	overnight	overnight	ADJ
fnp-5883	355	20	immersion	immersion	NOUN
fnp-5883	355	21	in	in	ADP
fnp-5883	355	22	30	30	NUM
fnp-5883	355	23	 	 	SPACE
fnp-5883	355	24	%	%	NOUN
fnp-5883	355	25	sucrose	sucrose	NOUN
fnp-5883	355	26	under	under	ADP
fnp-5883	355	27	refrigeration	refrigeration	NOUN
fnp-5883	355	28	before	before	ADP
fnp-5883	355	29	freezing	freeze	VERB
fnp-5883	355	30	in	in	ADP
fnp-5883	355	31	isopentane/2	isopentane/2	ADJ
fnp-5883	355	32	-	-	ADJ
fnp-5883	355	33	methylbutane	methylbutane	NOUN
fnp-5883	355	34	cooled	cool	VERB
fnp-5883	355	35	with	with	ADP
fnp-5883	355	36	liquid	liquid	ADJ
fnp-5883	355	37	nitrogen	nitrogen	NOUN
fnp-5883	355	38	(	(	PUNCT
fnp-5883	355	39	comba	comba	PROPN
fnp-5883	355	40	et	et	PROPN
fnp-5883	355	41	al	al	PROPN
fnp-5883	355	42	.	.	PROPN
fnp-5883	355	43	,	,	PUNCT
fnp-5883	355	44	2020	2020	NUM
fnp-5883	355	45	)	)	PUNCT
fnp-5883	355	46	.	.	PUNCT
fnp-5883	356	1	this	this	DET
fnp-5883	356	2	protocol	protocol	NOUN
fnp-5883	356	3	was	be	AUX
fnp-5883	356	4	found	find	VERB
fnp-5883	356	5	to	to	PART
fnp-5883	356	6	preserve	preserve	VERB
fnp-5883	356	7	morphology	morphology	NOUN
fnp-5883	356	8	in	in	ADP
fnp-5883	356	9	tumor	tumor	NOUN
fnp-5883	356	10	tissue	tissue	NOUN
fnp-5883	356	11	within	within	ADP
fnp-5883	356	12	the	the	DET
fnp-5883	356	13	brain	brain	NOUN
fnp-5883	356	14	as	as	SCONJ
fnp-5883	356	15	seen	see	VERB
fnp-5883	356	16	on	on	ADP
fnp-5883	356	17	light	light	ADJ
fnp-5883	356	18	microscopy	microscopy	NOUN
fnp-5883	356	19	,	,	PUNCT
fnp-5883	356	20	with	with	ADP
fnp-5883	356	21	no	no	DET
fnp-5883	356	22	histologically	histologically	ADV
fnp-5883	356	23	detectable	detectable	ADJ
fnp-5883	356	24	ice	ice	NOUN
fnp-5883	356	25	crystals	crystal	NOUN
fnp-5883	356	26	.	.	PUNCT
fnp-5883	357	1	the	the	DET
fnp-5883	357	2	extended	extend	VERB
fnp-5883	357	3	immersion	immersion	NOUN
fnp-5883	357	4	period	period	NOUN
fnp-5883	357	5	makes	make	VERB
fnp-5883	357	6	sense	sense	NOUN
fnp-5883	357	7	because	because	SCONJ
fnp-5883	357	8	perfusion	perfusion	NOUN
fnp-5883	357	9	of	of	ADP
fnp-5883	357	10	the	the	DET
fnp-5883	357	11	brain	brain	NOUN
fnp-5883	357	12	is	be	AUX
fnp-5883	357	13	not	not	PART
fnp-5883	357	14	completely	completely	ADV
fnp-5883	357	15	reliable	reliable	ADJ
fnp-5883	357	16	,	,	PUNCT
fnp-5883	357	17	even	even	ADV
fnp-5883	357	18	in	in	ADP
fnp-5883	357	19	ideal	ideal	ADJ
fnp-5883	357	20	,	,	PUNCT
fnp-5883	357	21	controlled	control	VERB
fnp-5883	357	22	laboratory	laboratory	NOUN
fnp-5883	357	23	conditions	condition	NOUN
fnp-5883	357	24	(	(	PUNCT
fnp-5883	357	25	cahill	cahill	PROPN
fnp-5883	357	26	et	et	PROPN
fnp-5883	357	27	al	al	PROPN
fnp-5883	357	28	.	.	PROPN
fnp-5883	357	29	,	,	PUNCT
fnp-5883	357	30	2012	2012	NUM
fnp-5883	357	31	)	)	PUNCT
fnp-5883	357	32	.	.	PUNCT
fnp-5883	358	1	although	although	SCONJ
fnp-5883	358	2	the	the	DET
fnp-5883	358	3	latter	latter	ADJ
fnp-5883	358	4	procedure	procedure	NOUN
fnp-5883	358	5	also	also	ADV
fnp-5883	358	6	raises	raise	VERB
fnp-5883	358	7	concerns	concern	NOUN
fnp-5883	358	8	about	about	ADP
fnp-5883	358	9	potential	potential	ADJ
fnp-5883	358	10	tissue	tissue	NOUN
fnp-5883	358	11	decomposition	decomposition	NOUN
fnp-5883	358	12	,	,	PUNCT
fnp-5883	358	13	the	the	DET
fnp-5883	358	14	authors	author	NOUN
fnp-5883	358	15	did	do	AUX
fnp-5883	358	16	not	not	PART
fnp-5883	358	17	report	report	VERB
fnp-5883	358	18	that	that	SCONJ
fnp-5883	358	19	the	the	DET
fnp-5883	358	20	extended	extended	ADJ
fnp-5883	358	21	immersion	immersion	NOUN
fnp-5883	358	22	period	period	NOUN
fnp-5883	358	23	affected	affect	VERB
fnp-5883	358	24	tissue	tissue	NOUN
fnp-5883	358	25	morphology	morphology	NOUN
fnp-5883	358	26	,	,	PUNCT
fnp-5883	358	27	consistent	consistent	ADJ
fnp-5883	358	28	with	with	ADP
fnp-5883	358	29	previous	previous	ADJ
fnp-5883	358	30	literature	literature	NOUN
fnp-5883	358	31	suggesting	suggest	VERB
fnp-5883	358	32	that	that	SCONJ
fnp-5883	358	33	the	the	DET
fnp-5883	358	34	cellular	cellular	ADJ
fnp-5883	358	35	effects	effect	NOUN
fnp-5883	358	36	of	of	ADP
fnp-5883	358	37	postmortem	postmortem	NOUN
fnp-5883	358	38	decomposition	decomposition	NOUN
fnp-5883	358	39	have	have	VERB
fnp-5883	358	40	a	a	DET
fnp-5883	358	41	relatively	relatively	ADV
fnp-5883	358	42	slow	slow	ADJ
fnp-5883	358	43	onset	onset	NOUN
fnp-5883	358	44	,	,	PUNCT
fnp-5883	358	45	especially	especially	ADV
fnp-5883	358	46	in	in	ADP
fnp-5883	358	47	refrigerated	refrigerated	ADJ
fnp-5883	358	48	tissue	tissue	NOUN
fnp-5883	358	49	(	(	PUNCT
fnp-5883	358	50	krassner	krassner	NOUN
fnp-5883	358	51	et	et	PROPN
fnp-5883	358	52	al	al	PROPN
fnp-5883	358	53	.	.	PROPN
fnp-5883	358	54	,	,	PUNCT
fnp-5883	358	55	2023	2023	NUM
fnp-5883	358	56	)	)	PUNCT
fnp-5883	358	57	.	.	PUNCT
fnp-5883	359	1	however	however	ADV
fnp-5883	359	2	,	,	PUNCT
fnp-5883	359	3	cryoprotectant	cryoprotectant	NOUN
fnp-5883	359	4	perfusion	perfusion	NOUN
fnp-5883	359	5	is	be	AUX
fnp-5883	359	6	expected	expect	VERB
fnp-5883	359	7	to	to	PART
fnp-5883	359	8	be	be	AUX
fnp-5883	359	9	more	more	ADV
fnp-5883	359	10	challenging	challenging	ADJ
fnp-5883	359	11	in	in	ADP
fnp-5883	359	12	a	a	DET
fnp-5883	359	13	brain	brain	NOUN
fnp-5883	359	14	banking	banking	NOUN
fnp-5883	359	15	context	context	NOUN
fnp-5883	359	16	,	,	PUNCT
fnp-5883	359	17	largely	largely	ADV
fnp-5883	359	18	as	as	ADP
fnp-5883	359	19	a	a	DET
fnp-5883	359	20	result	result	NOUN
fnp-5883	359	21	of	of	ADP
fnp-5883	359	22	agonal	agonal	ADJ
fnp-5883	359	23	and	and	CCONJ
fnp-5883	359	24	postmortem	postmortem	NOUN
fnp-5883	359	25	changes	change	NOUN
fnp-5883	359	26	(	(	PUNCT
fnp-5883	359	27	mcfadden	mcfadden	PROPN
fnp-5883	359	28	et	et	PROPN
fnp-5883	359	29	al	al	PROPN
fnp-5883	359	30	.	.	PROPN
fnp-5883	359	31	,	,	PUNCT
fnp-5883	359	32	2019	2019	NUM
fnp-5883	359	33	;	;	PUNCT
fnp-5883	359	34	mckenzie	mckenzie	PROPN
fnp-5883	359	35	et	et	PROPN
fnp-5883	359	36	al	al	PROPN
fnp-5883	359	37	.	.	PROPN
fnp-5883	359	38	,	,	PUNCT
fnp-5883	359	39	2022	2022	NUM
fnp-5883	359	40	)	)	PUNCT
fnp-5883	359	41	.	.	PUNCT
fnp-5883	360	1	sample	sample	NOUN
fnp-5883	360	2	type	type	NOUN
fnp-5883	360	3	(	(	PUNCT
fnp-5883	360	4	study	study	NOUN
fnp-5883	360	5	)	)	PUNCT
fnp-5883	360	6	cooling	cool	VERB
fnp-5883	360	7	method	method	NOUN
fnp-5883	360	8	visualization	visualization	NOUN
fnp-5883	360	9	method	method	NOUN
fnp-5883	360	10	key	key	ADJ
fnp-5883	360	11	findings	finding	NOUN
fnp-5883	360	12	whole	whole	ADJ
fnp-5883	360	13	cat	cat	NOUN
fnp-5883	360	14	brain	brain	NOUN
fnp-5883	360	15	within	within	ADP
fnp-5883	360	16	cranium	cranium	NOUN
fnp-5883	360	17	(	(	PUNCT
fnp-5883	360	18	suda	suda	NOUN
fnp-5883	360	19	et	et	PROPN
fnp-5883	360	20	al	al	PROPN
fnp-5883	360	21	.	.	PROPN
fnp-5883	360	22	,	,	PUNCT
fnp-5883	360	23	1966	1966	NUM
fnp-5883	360	24	)	)	PUNCT
fnp-5883	360	25	perfusion	perfusion	NOUN
fnp-5883	360	26	with	with	ADP
fnp-5883	360	27	15	15	NUM
fnp-5883	360	28	 	 	SPACE
fnp-5883	360	29	%	%	NOUN
fnp-5883	360	30	glycerol	glycerol	NOUN
fnp-5883	360	31	,	,	PUNCT
fnp-5883	360	32	then	then	ADV
fnp-5883	360	33	placed	place	VERB
fnp-5883	360	34	in	in	ADP
fnp-5883	360	35	a	a	DET
fnp-5883	360	36	‑20	‑20	PRON
fnp-5883	360	37	 	 	SPACE
fnp-5883	360	38	°	°	ADP
fnp-5883	360	39	c	c	NOUN
fnp-5883	360	40	freezer	freezer	NOUN
fnp-5883	360	41	light	light	NOUN
fnp-5883	360	42	microscopy	microscopy	NOUN
fnp-5883	360	43	with	with	ADP
fnp-5883	360	44	nissl	nissl	ADJ
fnp-5883	360	45	staining	staining	NOUN
fnp-5883	360	46	reported	report	VERB
fnp-5883	360	47	to	to	PART
fnp-5883	360	48	have	have	VERB
fnp-5883	360	49	"	"	PUNCT
fnp-5883	360	50	almost	almost	ADV
fnp-5883	360	51	normal	normal	ADJ
fnp-5883	360	52	cell	cell	NOUN
fnp-5883	360	53	arrangements	arrangement	NOUN
fnp-5883	360	54	"	"	PUNCT
fnp-5883	360	55	whole	whole	ADJ
fnp-5883	360	56	rat	rat	NOUN
fnp-5883	360	57	brain	brain	NOUN
fnp-5883	360	58	(	(	PUNCT
fnp-5883	360	59	canatelli	canatelli	PROPN
fnp-5883	360	60	-	-	PUNCT
fnp-5883	360	61	mallat	mallat	PROPN
fnp-5883	360	62	et	et	PROPN
fnp-5883	360	63	al	al	PROPN
fnp-5883	360	64	.	.	PROPN
fnp-5883	360	65	,	,	PUNCT
fnp-5883	360	66	2020	2020	NUM
fnp-5883	360	67	)	)	PUNCT
fnp-5883	360	68	intracardial	intracardial	ADJ
fnp-5883	360	69	perfusion	perfusion	NOUN
fnp-5883	360	70	at	at	ADP
fnp-5883	360	71	4	4	NUM
fnp-5883	360	72	 	 	SPACE
fnp-5883	360	73	°	°	NOUN
fnp-5883	360	74	c	c	NOUN
fnp-5883	360	75	with	with	ADP
fnp-5883	360	76	increasing	increase	VERB
fnp-5883	360	77	cpa	cpa	NOUN
fnp-5883	360	78	concentration	concentration	NOUN
fnp-5883	360	79	,	,	PUNCT
fnp-5883	360	80	up	up	ADP
fnp-5883	360	81	to	to	PART
fnp-5883	360	82	vm1	vm1	VERB
fnp-5883	360	83	vitrification	vitrification	NOUN
fnp-5883	360	84	solution	solution	NOUN
fnp-5883	360	85	immersed	immerse	VERB
fnp-5883	360	86	in	in	ADP
fnp-5883	360	87	fixative	fixative	NOUN
fnp-5883	360	88	at	at	ADP
fnp-5883	360	89	‑20	‑20	PRON
fnp-5883	360	90	 	 	SPACE
fnp-5883	360	91	°	°	NOUN
fnp-5883	360	92	c	c	NOUN
fnp-5883	360	93	,	,	PUNCT
fnp-5883	360	94	light	light	ADJ
fnp-5883	360	95	microscopy	microscopy	NOUN
fnp-5883	360	96	with	with	ADP
fnp-5883	360	97	immuno	immuno	ADJ
fnp-5883	360	98	-	-	PUNCT
fnp-5883	360	99	histochemistry	histochemistry	NOUN
fnp-5883	360	100	no	no	DET
fnp-5883	360	101	histologic	histologic	ADJ
fnp-5883	360	102	evidence	evidence	NOUN
fnp-5883	360	103	of	of	ADP
fnp-5883	360	104	ice	ice	NOUN
fnp-5883	360	105	formation	formation	NOUN
fnp-5883	360	106	,	,	PUNCT
fnp-5883	360	107	well	well	ADV
fnp-5883	360	108	preserved	preserve	VERB
fnp-5883	360	109	synaptic	synaptic	ADJ
fnp-5883	360	110	immunostaining	immunostaining	NOUN
fnp-5883	360	111	,	,	PUNCT
fnp-5883	360	112	fainter	faint	ADJ
fnp-5883	360	113	neun	neun	NOUN
fnp-5883	360	114	staining	staining	ADJ
fnp-5883	360	115	,	,	PUNCT
fnp-5883	360	116	shrinkage	shrinkage	NOUN
fnp-5883	360	117	of	of	ADP
fnp-5883	360	118	neurons	neuron	NOUN
fnp-5883	360	119	whole	whole	ADJ
fnp-5883	360	120	mouse	mouse	NOUN
fnp-5883	360	121	brain	brain	NOUN
fnp-5883	360	122	(	(	PUNCT
fnp-5883	360	123	comba	comba	NOUN
fnp-5883	360	124	et	et	PROPN
fnp-5883	360	125	al	al	PROPN
fnp-5883	360	126	.	.	PROPN
fnp-5883	360	127	,	,	PUNCT
fnp-5883	360	128	2020	2020	NUM
fnp-5883	360	129	)	)	PUNCT
fnp-5883	360	130	perfusion	perfusion	NOUN
fnp-5883	360	131	with	with	ADP
fnp-5883	360	132	30	30	NUM
fnp-5883	360	133	 	 	SPACE
fnp-5883	360	134	%	%	NOUN
fnp-5883	360	135	sucrose	sucrose	NOUN
fnp-5883	360	136	,	,	PUNCT
fnp-5883	360	137	then	then	ADV
fnp-5883	360	138	overnight	overnight	ADJ
fnp-5883	360	139	immersion	immersion	NOUN
fnp-5883	360	140	in	in	ADP
fnp-5883	360	141	30	30	NUM
fnp-5883	360	142	 	 	SPACE
fnp-5883	360	143	%	%	NOUN
fnp-5883	360	144	sucrose	sucrose	NOUN
fnp-5883	360	145	,	,	PUNCT
fnp-5883	360	146	embedded	embed	VERB
fnp-5883	360	147	in	in	ADP
fnp-5883	360	148	oct	oct	PROPN
fnp-5883	360	149	compound	compound	NOUN
fnp-5883	360	150	,	,	PUNCT
fnp-5883	360	151	and	and	CCONJ
fnp-5883	360	152	frozen	freeze	VERB
fnp-5883	360	153	in	in	ADP
fnp-5883	360	154	isopentane/2	isopentane/2	NOUN
fnp-5883	360	155	-	-	ADJ
fnp-5883	360	156	methylbutane	methylbutane	NOUN
fnp-5883	360	157	cooled	cool	VERB
fnp-5883	360	158	with	with	ADP
fnp-5883	360	159	liquid	liquid	ADJ
fnp-5883	360	160	nitrogen	nitrogen	NOUN
fnp-5883	360	161	cryostat	cryostat	NOUN
fnp-5883	360	162	sectioning	section	VERB
fnp-5883	360	163	at	at	ADP
fnp-5883	360	164	‑20	‑20	PRON
fnp-5883	360	165	to	to	ADP
fnp-5883	360	166	‑24	‑24	NOUN
fnp-5883	360	167	 	 	SPACE
fnp-5883	360	168	°	°	NOUN
fnp-5883	360	169	c	c	NOUN
fnp-5883	360	170	,	,	PUNCT
fnp-5883	360	171	ethanol	ethanol	NOUN
fnp-5883	360	172	fixation	fixation	NOUN
fnp-5883	360	173	,	,	PUNCT
fnp-5883	360	174	light	light	ADJ
fnp-5883	360	175	microscopy	microscopy	NOUN
fnp-5883	360	176	after	after	ADP
fnp-5883	360	177	h&e	h&e	PROPN
fnp-5883	360	178	staining	stain	VERB
fnp-5883	360	179	no	no	DET
fnp-5883	360	180	ice	ice	NOUN
fnp-5883	360	181	crystals	crystal	NOUN
fnp-5883	360	182	observed	observe	VERB
fnp-5883	360	183	on	on	ADP
fnp-5883	360	184	light	light	ADJ
fnp-5883	360	185	microscopy	microscopy	NOUN
fnp-5883	360	186	,	,	PUNCT
fnp-5883	360	187	morphology	morphology	NOUN
fnp-5883	360	188	and	and	CCONJ
fnp-5883	360	189	rna	rna	NOUN
fnp-5883	360	190	integrity	integrity	NOUN
fnp-5883	360	191	of	of	ADP
fnp-5883	360	192	tissue	tissue	NOUN
fnp-5883	360	193	reported	report	VERB
fnp-5883	360	194	to	to	PART
fnp-5883	360	195	be	be	AUX
fnp-5883	360	196	preserved	preserve	VERB
fnp-5883	360	197	table	table	NOUN
fnp-5883	360	198	 	 	SPACE
fnp-5883	360	199	5	5	NUM
fnp-5883	360	200	.	.	PUNCT
fnp-5883	360	201	reported	report	VERB
fnp-5883	360	202	histologic	histologic	ADJ
fnp-5883	360	203	effects	effect	NOUN
fnp-5883	360	204	in	in	ADP
fnp-5883	360	205	representative	representative	ADJ
fnp-5883	360	206	observations	observation	NOUN
fnp-5883	360	207	from	from	ADP
fnp-5883	360	208	studies	study	NOUN
fnp-5883	360	209	using	use	VERB
fnp-5883	360	210	cryopreservation	cryopreservation	NOUN
fnp-5883	360	211	with	with	ADP
fnp-5883	360	212	cryoprotectant	cryoprotectant	NOUN
fnp-5883	360	213	perfusion	perfusion	NOUN
fnp-5883	360	214	.	.	PUNCT
fnp-5883	361	1	vm1	vm1	NOUN
fnp-5883	361	2	:	:	PUNCT
fnp-5883	361	3	vitrification	vitrification	NOUN
fnp-5883	361	4	solution	solution	NOUN
fnp-5883	361	5	;	;	PUNCT
fnp-5883	361	6	neun	neun	NOUN
fnp-5883	361	7	:	:	PUNCT
fnp-5883	361	8	neuronal	neuronal	ADJ
fnp-5883	361	9	nuclei	nucleus	NOUN
fnp-5883	361	10	,	,	PUNCT
fnp-5883	361	11	a	a	DET
fnp-5883	361	12	neuronal	neuronal	ADJ
fnp-5883	361	13	marker	marker	NOUN
fnp-5883	361	14	protein	protein	NOUN
fnp-5883	361	15	;	;	PUNCT
fnp-5883	361	16	oct	oct	PROPN
fnp-5883	361	17	:	:	PUNCT
fnp-5883	361	18	optimal	optimal	ADJ
fnp-5883	361	19	cutting	cut	VERB
fnp-5883	361	20	temperature	temperature	NOUN
fnp-5883	361	21	;	;	PUNCT
fnp-5883	361	22	h&e	h&e	PROPN
fnp-5883	361	23	:	:	PUNCT
fnp-5883	361	24	hematoxylin	hematoxylin	PROPN
fnp-5883	361	25	and	and	CCONJ
fnp-5883	361	26	eosin	eosin	NOUN
fnp-5883	361	27	;	;	PUNCT
fnp-5883	361	28	cpa	cpa	NOUN
fnp-5883	361	29	:	:	PUNCT
fnp-5883	361	30	cryoprotective	cryoprotective	ADJ
fnp-5883	361	31	agent	agent	NOUN
fnp-5883	361	32	.	.	PUNCT
fnp-5883	362	1	summary	summary	NOUN
fnp-5883	362	2	cryoprotectants	cryoprotectant	NOUN
fnp-5883	362	3	like	like	VERB
fnp-5883	362	4	dmso	dmso	NOUN
fnp-5883	362	5	,	,	PUNCT
fnp-5883	362	6	glycerol	glycerol	NOUN
fnp-5883	362	7	,	,	PUNCT
fnp-5883	362	8	and	and	CCONJ
fnp-5883	362	9	sucrose	sucrose	NOUN
fnp-5883	362	10	can	can	AUX
fnp-5883	362	11	reduce	reduce	VERB
fnp-5883	362	12	ice	ice	NOUN
fnp-5883	362	13	formation	formation	NOUN
fnp-5883	362	14	during	during	ADP
fnp-5883	362	15	cryopreservation	cryopreservation	NOUN
fnp-5883	362	16	and	and	CCONJ
fnp-5883	362	17	potentially	potentially	ADV
fnp-5883	362	18	enable	enable	ADJ
fnp-5883	362	19	vitrification	vitrification	NOUN
fnp-5883	362	20	.	.	PUNCT
fnp-5883	363	1	high	high	ADJ
fnp-5883	363	2	cryoprotectant	cryoprotectant	NOUN
fnp-5883	363	3	concentrations	concentration	NOUN
fnp-5883	363	4	are	be	AUX
fnp-5883	363	5	needed	need	VERB
fnp-5883	363	6	for	for	ADP
fnp-5883	363	7	complete	complete	ADJ
fnp-5883	363	8	vitrification	vitrification	NOUN
fnp-5883	363	9	,	,	PUNCT
fnp-5883	363	10	which	which	PRON
fnp-5883	363	11	can	can	AUX
fnp-5883	363	12	cause	cause	VERB
fnp-5883	363	13	osmotic	osmotic	ADJ
fnp-5883	363	14	stress	stress	NOUN
fnp-5883	363	15	if	if	SCONJ
fnp-5883	363	16	the	the	DET
fnp-5883	363	17	procedure	procedure	NOUN
fnp-5883	363	18	is	be	AUX
fnp-5883	363	19	not	not	PART
fnp-5883	363	20	performed	perform	VERB
fnp-5883	363	21	in	in	ADP
fnp-5883	363	22	a	a	DET
fnp-5883	363	23	properly	properly	ADV
fnp-5883	363	24	graded	grade	VERB
fnp-5883	363	25	manner	manner	NOUN
fnp-5883	363	26	.	.	PUNCT
fnp-5883	364	1	immersion	immersion	NOUN
fnp-5883	364	2	of	of	ADP
fnp-5883	364	3	brain	brain	NOUN
fnp-5883	364	4	tissue	tissue	NOUN
fnp-5883	364	5	in	in	ADP
fnp-5883	364	6	cryoprotectants	cryoprotectant	NOUN
fnp-5883	364	7	has	have	AUX
fnp-5883	364	8	been	be	AUX
fnp-5883	364	9	reported	report	VERB
fnp-5883	364	10	to	to	PART
fnp-5883	364	11	preserve	preserve	VERB
fnp-5883	364	12	ultrastructure	ultrastructure	NOUN
fnp-5883	364	13	,	,	PUNCT
fnp-5883	364	14	but	but	CCONJ
fnp-5883	364	15	certain	certain	ADJ
fnp-5883	364	16	areas	area	NOUN
fnp-5883	364	17	can	can	AUX
fnp-5883	364	18	still	still	ADV
fnp-5883	364	19	have	have	VERB
fnp-5883	364	20	freezing	freeze	VERB
fnp-5883	364	21	artifacts	artifact	NOUN
fnp-5883	364	22	if	if	SCONJ
fnp-5883	364	23	cryoprotectant	cryoprotectant	NOUN
fnp-5883	364	24	diffusion	diffusion	NOUN
fnp-5883	364	25	or	or	CCONJ
fnp-5883	364	26	uptake	uptake	ADJ
fnp-5883	364	27	by	by	ADP
fnp-5883	364	28	cells	cell	NOUN
fnp-5883	364	29	are	be	AUX
fnp-5883	364	30	incomplete	incomplete	ADJ
fnp-5883	364	31	.	.	PUNCT
fnp-5883	365	1	perfusion	perfusion	NOUN
fnp-5883	365	2	of	of	ADP
fnp-5883	365	3	cryoprotectants	cryoprotectant	NOUN
fnp-5883	365	4	through	through	ADP
fnp-5883	365	5	the	the	DET
fnp-5883	365	6	cerebrovascular	cerebrovascular	ADJ
fnp-5883	365	7	system	system	NOUN
fnp-5883	365	8	may	may	AUX
fnp-5883	365	9	enable	enable	VERB
fnp-5883	365	10	more	more	ADJ
fnp-5883	365	11	uniform	uniform	ADJ
fnp-5883	365	12	cryoprotectant	cryoprotectant	NOUN
fnp-5883	365	13	distribution	distribution	NOUN
fnp-5883	365	14	and	and	CCONJ
fnp-5883	365	15	better	well	ADJ
fnp-5883	365	16	tissue	tissue	NOUN
fnp-5883	365	17	preservation	preservation	NOUN
fnp-5883	365	18	in	in	ADP
fnp-5883	365	19	larger	large	ADJ
fnp-5883	365	20	brain	brain	NOUN
fnp-5883	365	21	samples	sample	NOUN
fnp-5883	365	22	,	,	PUNCT
fnp-5883	365	23	but	but	CCONJ
fnp-5883	365	24	this	this	DET
fnp-5883	365	25	approach	approach	NOUN
fnp-5883	365	26	is	be	AUX
fnp-5883	365	27	challenging	challenge	VERB
fnp-5883	365	28	to	to	PART
fnp-5883	365	29	perform	perform	VERB
fnp-5883	365	30	,	,	PUNCT
fnp-5883	365	31	especially	especially	ADV
fnp-5883	365	32	in	in	ADP
fnp-5883	365	33	postmortem	postmortem	NOUN
fnp-5883	365	34	brain	brain	NOUN
fnp-5883	365	35	banking	banking	NOUN
fnp-5883	365	36	settings	setting	NOUN
fnp-5883	365	37	,	,	PUNCT
fnp-5883	365	38	and	and	CCONJ
fnp-5883	365	39	the	the	DET
fnp-5883	365	40	evidence	evidence	NOUN
fnp-5883	365	41	for	for	ADP
fnp-5883	365	42	its	its	PRON
fnp-5883	365	43	efficacy	efficacy	NOUN
fnp-5883	365	44	in	in	ADP
fnp-5883	365	45	the	the	DET
fnp-5883	365	46	published	publish	VERB
fnp-5883	365	47	literature	literature	NOUN
fnp-5883	365	48	is	be	AUX
fnp-5883	365	49	currently	currently	ADV
fnp-5883	365	50	limited	limited	ADJ
fnp-5883	365	51	.	.	PUNCT
fnp-5883	366	1	storage	storage	NOUN
fnp-5883	366	2	temperature	temperature	NOUN
fnp-5883	366	3	and	and	CCONJ
fnp-5883	366	4	length	length	NOUN
fnp-5883	366	5	one	one	NUM
fnp-5883	366	6	previous	previous	ADJ
fnp-5883	366	7	source	source	NOUN
fnp-5883	366	8	has	have	AUX
fnp-5883	366	9	comprehensively	comprehensively	ADV
fnp-5883	366	10	reviewed	review	VERB
fnp-5883	366	11	the	the	DET
fnp-5883	366	12	literature	literature	NOUN
fnp-5883	366	13	on	on	ADP
fnp-5883	366	14	cryostorage	cryostorage	NOUN
fnp-5883	366	15	(	(	PUNCT
fnp-5883	366	16	hubel	hubel	VERB
fnp-5883	366	17	et	et	PROPN
fnp-5883	366	18	al	al	PROPN
fnp-5883	366	19	.	.	PROPN
fnp-5883	366	20	,	,	PUNCT
fnp-5883	366	21	2014	2014	NUM
fnp-5883	366	22	)	)	PUNCT
fnp-5883	366	23	.	.	PUNCT
fnp-5883	367	1	the	the	DET
fnp-5883	367	2	authors	author	NOUN
fnp-5883	367	3	report	report	VERB
fnp-5883	367	4	that	that	SCONJ
fnp-5883	367	5	unfixed	unfixed	ADJ
fnp-5883	367	6	biospecimens	biospecimen	NOUN
fnp-5883	367	7	kept	keep	VERB
fnp-5883	367	8	above	above	ADP
fnp-5883	367	9	the	the	DET
fnp-5883	367	10	tissue	tissue	NOUN
fnp-5883	367	11	’s	’s	PART
fnp-5883	367	12	glass	glass	NOUN
fnp-5883	367	13	transition	transition	NOUN
fnp-5883	367	14	temperature	temperature	NOUN
fnp-5883	367	15	are	be	AUX
fnp-5883	367	16	expected	expect	VERB
fnp-5883	367	17	to	to	PART
fnp-5883	367	18	show	show	VERB
fnp-5883	367	19	degradation	degradation	NOUN
fnp-5883	367	20	,	,	PUNCT
fnp-5883	367	21	even	even	ADV
fnp-5883	367	22	at	at	ADP
fnp-5883	367	23	the	the	DET
fnp-5883	367	24	ultra	ultra	ADJ
fnp-5883	367	25	-	-	ADJ
fnp-5883	367	26	low	low	ADJ
fnp-5883	367	27	freezer	freezer	NOUN
fnp-5883	367	28	temperature	temperature	NOUN
fnp-5883	367	29	of	of	ADP
fnp-5883	367	30	‑80	‑80	NOUN
fnp-5883	367	31	 	 	SPACE
fnp-5883	367	32	°	°	ADP
fnp-5883	367	33	c	c	NOUN
fnp-5883	367	34	.	.	PUNCT
fnp-5883	368	1	there	there	PRON
fnp-5883	368	2	are	be	VERB
fnp-5883	368	3	several	several	ADJ
fnp-5883	368	4	reasons	reason	NOUN
fnp-5883	368	5	for	for	ADP
fnp-5883	368	6	this	this	PRON
fnp-5883	368	7	.	.	PUNCT
fnp-5883	369	1	first	first	ADV
fnp-5883	369	2	,	,	PUNCT
fnp-5883	369	3	the	the	DET
fnp-5883	369	4	presence	presence	NOUN
fnp-5883	369	5	of	of	ADP
fnp-5883	369	6	residual	residual	ADJ
fnp-5883	369	7	liquid	liquid	ADJ
fnp-5883	369	8	water	water	NOUN
fnp-5883	369	9	allows	allow	VERB
fnp-5883	369	10	for	for	ADP
fnp-5883	369	11	ongoing	ongoing	ADJ
fnp-5883	369	12	molecular	molecular	ADJ
fnp-5883	369	13	motion	motion	NOUN
fnp-5883	369	14	.	.	PUNCT
fnp-5883	370	1	second	second	ADJ
fnp-5883	370	2	,	,	PUNCT
fnp-5883	370	3	degradative	degradative	ADJ
fnp-5883	370	4	biomolecules	biomolecule	NOUN
fnp-5883	370	5	,	,	PUNCT
fnp-5883	370	6	such	such	ADJ
fnp-5883	370	7	as	as	ADP
fnp-5883	370	8	proteases	protease	NOUN
fnp-5883	370	9	,	,	PUNCT
fnp-5883	370	10	nucleases	nuclease	NOUN
fnp-5883	370	11	,	,	PUNCT
fnp-5883	370	12	and	and	CCONJ
fnp-5883	370	13	lipases	lipase	NOUN
fnp-5883	370	14	,	,	PUNCT
fnp-5883	370	15	may	may	AUX
fnp-5883	370	16	still	still	ADV
fnp-5883	370	17	have	have	VERB
fnp-5883	370	18	activity	activity	NOUN
fnp-5883	370	19	,	,	PUNCT
fnp-5883	370	20	which	which	PRON
fnp-5883	370	21	can	can	AUX
fnp-5883	370	22	lead	lead	VERB
fnp-5883	370	23	to	to	ADP
fnp-5883	370	24	biochemical	biochemical	ADJ
fnp-5883	370	25	alterations	alteration	NOUN
fnp-5883	370	26	.	.	PUNCT
fnp-5883	371	1	although	although	SCONJ
fnp-5883	371	2	the	the	DET
fnp-5883	371	3	activity	activity	NOUN
fnp-5883	371	4	of	of	ADP
fnp-5883	371	5	degradative	degradative	ADJ
fnp-5883	371	6	enzymes	enzyme	NOUN
fnp-5883	371	7	is	be	AUX
fnp-5883	371	8	expected	expect	VERB
fnp-5883	371	9	to	to	PART
fnp-5883	371	10	be	be	AUX
fnp-5883	371	11	greatly	greatly	ADV
fnp-5883	371	12	reduced	reduce	VERB
fnp-5883	371	13	,	,	PUNCT
fnp-5883	371	14	it	it	PRON
fnp-5883	371	15	is	be	AUX
fnp-5883	371	16	not	not	PART
fnp-5883	371	17	necessarily	necessarily	ADV
fnp-5883	371	18	completely	completely	ADV
fnp-5883	371	19	halted	halt	VERB
fnp-5883	371	20	at	at	ADP
fnp-5883	371	21	‑80	‑80	NOUN
fnp-5883	371	22	 	 	SPACE
fnp-5883	371	23	°	°	ADP
fnp-5883	371	24	c	c	NOUN
fnp-5883	371	25	,	,	PUNCT
fnp-5883	371	26	and	and	CCONJ
fnp-5883	371	27	this	this	PRON
fnp-5883	371	28	can	can	AUX
fnp-5883	371	29	add	add	VERB
fnp-5883	371	30	up	up	ADP
fnp-5883	371	31	over	over	ADP
fnp-5883	371	32	long	long	ADJ
fnp-5883	371	33	periods	period	NOUN
fnp-5883	371	34	of	of	ADP
fnp-5883	371	35	storage	storage	NOUN
fnp-5883	371	36	.	.	PUNCT
fnp-5883	372	1	third	third	ADJ
fnp-5883	372	2	,	,	PUNCT
fnp-5883	372	3	ice	ice	NOUN
fnp-5883	372	4	crystals	crystal	NOUN
fnp-5883	372	5	will	will	AUX
fnp-5883	372	6	become	become	VERB
fnp-5883	372	7	larger	large	ADJ
fnp-5883	372	8	during	during	ADP
fnp-5883	372	9	long	long	ADJ
fnp-5883	372	10	-	-	PUNCT
fnp-5883	372	11	term	term	NOUN
fnp-5883	372	12	storage	storage	NOUN
fnp-5883	372	13	,	,	PUNCT
fnp-5883	372	14	leading	lead	VERB
fnp-5883	372	15	to	to	ADP
fnp-5883	372	16	more	more	ADJ
fnp-5883	372	17	damage	damage	NOUN
fnp-5883	372	18	to	to	ADP
fnp-5883	372	19	tissue	tissue	NOUN
fnp-5883	372	20	morphology	morphology	NOUN
fnp-5883	372	21	(	(	PUNCT
fnp-5883	372	22	choi	choi	NOUN
fnp-5883	372	23	et	et	PROPN
fnp-5883	372	24	al	al	PROPN
fnp-5883	372	25	.	.	PROPN
fnp-5883	372	26	,	,	PUNCT
fnp-5883	372	27	2015	2015	NUM
fnp-5883	372	28	;	;	PUNCT
fnp-5883	372	29	zennoune	zennoune	NOUN
fnp-5883	372	30	et	et	PROPN
fnp-5883	372	31	al	al	PROPN
fnp-5883	372	32	.	.	PROPN
fnp-5883	372	33	,	,	PUNCT
fnp-5883	372	34	2022	2022	NUM
fnp-5883	372	35	)	)	PUNCT
fnp-5883	372	36	.	.	PUNCT
fnp-5883	373	1	such	such	ADJ
fnp-5883	373	2	ice	ice	NOUN
fnp-5883	373	3	recrystallization	recrystallization	NOUN
fnp-5883	373	4	is	be	AUX
fnp-5883	373	5	expected	expect	VERB
fnp-5883	373	6	to	to	PART
fnp-5883	373	7	be	be	AUX
fnp-5883	373	8	especially	especially	ADV
fnp-5883	373	9	common	common	ADJ
fnp-5883	373	10	as	as	ADP
fnp-5883	373	11	a	a	DET
fnp-5883	373	12	result	result	NOUN
fnp-5883	373	13	of	of	ADP
fnp-5883	373	14	temperature	temperature	NOUN
fnp-5883	373	15	fluctuations	fluctuation	NOUN
fnp-5883	373	16	(	(	PUNCT
fnp-5883	373	17	petzold	petzold	NOUN
fnp-5883	373	18	and	and	CCONJ
fnp-5883	373	19	aguilera	aguilera	NOUN
fnp-5883	373	20	,	,	PUNCT
fnp-5883	373	21	2009	2009	NUM
fnp-5883	373	22	)	)	PUNCT
fnp-5883	373	23	.	.	PUNCT
fnp-5883	374	1	all	all	DET
fnp-5883	374	2	these	these	DET
fnp-5883	374	3	factors	factor	NOUN
fnp-5883	374	4	add	add	VERB
fnp-5883	374	5	up	up	ADP
fnp-5883	374	6	to	to	PART
fnp-5883	374	7	potentially	potentially	ADV
fnp-5883	374	8	damage	damage	VERB
fnp-5883	374	9	tissue	tissue	NOUN
fnp-5883	374	10	during	during	ADP
fnp-5883	374	11	storage	storage	NOUN
fnp-5883	374	12	at	at	ADP
fnp-5883	374	13	temperatures	temperature	NOUN
fnp-5883	374	14	above	above	ADP
fnp-5883	374	15	the	the	DET
fnp-5883	374	16	glass	glass	NOUN
fnp-5883	374	17	transition	transition	NOUN
fnp-5883	374	18	temperature	temperature	NOUN
fnp-5883	374	19	.	.	PUNCT
fnp-5883	375	1	this	this	PRON
fnp-5883	375	2	has	have	AUX
fnp-5883	375	3	been	be	AUX
fnp-5883	375	4	borne	bear	VERB
fnp-5883	375	5	out	out	ADP
fnp-5883	375	6	empirically	empirically	ADV
fnp-5883	375	7	in	in	ADP
fnp-5883	375	8	some	some	PRON
fnp-5883	375	9	,	,	PUNCT
fnp-5883	375	10	but	but	CCONJ
fnp-5883	375	11	not	not	PART
fnp-5883	375	12	all	all	DET
fnp-5883	375	13	studies	study	NOUN
fnp-5883	375	14	.	.	PUNCT
fnp-5883	376	1	one	one	NUM
fnp-5883	376	2	study	study	NOUN
fnp-5883	376	3	found	find	VERB
fnp-5883	376	4	that	that	SCONJ
fnp-5883	376	5	human	human	ADJ
fnp-5883	376	6	brain	brain	NOUN
fnp-5883	376	7	tissue	tissue	NOUN
fnp-5883	376	8	stored	store	VERB
fnp-5883	376	9	at	at	ADP
fnp-5883	376	10	‑70	‑70	PROPN
fnp-5883	376	11	 	 	SPACE
fnp-5883	376	12	°	°	ADP
fnp-5883	376	13	c	c	NOUN
fnp-5883	376	14	for	for	ADP
fnp-5883	376	15	more	more	ADJ
fnp-5883	376	16	than	than	ADP
fnp-5883	376	17	one	one	NUM
fnp-5883	376	18	year	year	NOUN
fnp-5883	376	19	developed	develop	VERB
fnp-5883	376	20	irregular	irregular	ADJ
fnp-5883	376	21	spaces	space	NOUN
fnp-5883	376	22	and	and	CCONJ
fnp-5883	376	23	variations	variation	NOUN
fnp-5883	376	24	in	in	ADP
fnp-5883	376	25	section	section	NOUN
fnp-5883	376	26	thickness	thickness	NOUN
fnp-5883	376	27	that	that	PRON
fnp-5883	376	28	hindered	hinder	VERB
fnp-5883	376	29	comparative	comparative	ADJ
fnp-5883	376	30	analyses	analysis	NOUN
fnp-5883	376	31	(	(	PUNCT
fnp-5883	376	32	itoyama	itoyama	NOUN
fnp-5883	376	33	et	et	PROPN
fnp-5883	376	34	al	al	PROPN
fnp-5883	376	35	.	.	PROPN
fnp-5883	376	36	,	,	PUNCT
fnp-5883	376	37	1980	1980	NUM
fnp-5883	376	38	)	)	PUNCT
fnp-5883	376	39	.	.	PUNCT
fnp-5883	377	1	another	another	DET
fnp-5883	377	2	study	study	NOUN
fnp-5883	377	3	of	of	ADP
fnp-5883	377	4	dog	dog	NOUN
fnp-5883	377	5	brain	brain	NOUN
fnp-5883	377	6	samples	sample	NOUN
fnp-5883	377	7	held	hold	VERB
fnp-5883	377	8	at	at	ADP
fnp-5883	377	9	‑18	‑18	NOUN
fnp-5883	377	10	 	 	SPACE
fnp-5883	377	11	°	°	NOUN
fnp-5883	377	12	c	c	PROPN
fnp-5883	377	13	found	find	VERB
fnp-5883	377	14	that	that	SCONJ
fnp-5883	377	15	this	this	PRON
fnp-5883	377	16	led	lead	VERB
fnp-5883	377	17	to	to	ADP
fnp-5883	377	18	increased	increase	VERB
fnp-5883	377	19	damage	damage	NOUN
fnp-5883	377	20	to	to	ADP
fnp-5883	377	21	neurons	neuron	NOUN
fnp-5883	377	22	and	and	CCONJ
fnp-5883	377	23	to	to	PART
fnp-5883	377	24	increased	increase	VERB
fnp-5883	377	25	large	large	ADJ
fnp-5883	377	26	,	,	PUNCT
fnp-5883	377	27	irregular	irregular	ADJ
fnp-5883	377	28	,	,	PUNCT
fnp-5883	377	29	clear	clear	ADJ
fnp-5883	377	30	areas	area	NOUN
fnp-5883	377	31	under	under	ADP
fnp-5883	377	32	the	the	DET
fnp-5883	377	33	microscope	microscope	NOUN
fnp-5883	377	34	after	after	ADP
fnp-5883	377	35	7	7	NUM
fnp-5883	377	36	days	day	NOUN
fnp-5883	377	37	,	,	PUNCT
fnp-5883	377	38	as	as	SCONJ
fnp-5883	377	39	compared	compare	VERB
fnp-5883	377	40	to	to	ADP
fnp-5883	377	41	2	2	NUM
fnp-5883	377	42	days	day	NOUN
fnp-5883	377	43	of	of	ADP
fnp-5883	377	44	storage	storage	NOUN
fnp-5883	377	45	(	(	PUNCT
fnp-5883	377	46	baraibar	baraibar	NOUN
fnp-5883	377	47	and	and	CCONJ
fnp-5883	377	48	schoning	schoning	NOUN
fnp-5883	377	49	,	,	PUNCT
fnp-5883	377	50	1985	1985	NUM
fnp-5883	377	51	)	)	PUNCT
fnp-5883	377	52	.	.	PUNCT
fnp-5883	378	1	notably	notably	ADV
fnp-5883	378	2	,	,	PUNCT
fnp-5883	378	3	in	in	ADP
fnp-5883	378	4	this	this	DET
fnp-5883	378	5	study	study	NOUN
fnp-5883	378	6	,	,	PUNCT
fnp-5883	378	7	hemolysis	hemolysis	NOUN
fnp-5883	378	8	was	be	AUX
fnp-5883	378	9	already	already	ADV
fnp-5883	378	10	complete	complete	ADJ
fnp-5883	378	11	by	by	ADP
fnp-5883	378	12	2	2	NUM
fnp-5883	378	13	days	day	NOUN
fnp-5883	378	14	,	,	PUNCT
fnp-5883	378	15	so	so	SCONJ
fnp-5883	378	16	it	it	PRON
fnp-5883	378	17	did	do	AUX
fnp-5883	378	18	not	not	PART
fnp-5883	378	19	worsen	worsen	VERB
fnp-5883	378	20	by	by	ADP
fnp-5883	378	21	7	7	NUM
fnp-5883	378	22	days	day	NOUN
fnp-5883	378	23	of	of	ADP
fnp-5883	378	24	storage	storage	NOUN
fnp-5883	378	25	.	.	PUNCT
fnp-5883	379	1	however	however	ADV
fnp-5883	379	2	,	,	PUNCT
fnp-5883	379	3	other	other	ADJ
fnp-5883	379	4	studies	study	NOUN
fnp-5883	379	5	have	have	AUX
fnp-5883	379	6	found	find	VERB
fnp-5883	379	7	that	that	SCONJ
fnp-5883	379	8	histomorphological	histomorphological	ADJ
fnp-5883	379	9	features	feature	NOUN
fnp-5883	379	10	were	be	AUX
fnp-5883	379	11	not	not	PART
fnp-5883	379	12	altered	alter	VERB
fnp-5883	379	13	after	after	ADP
fnp-5883	379	14	years	year	NOUN
fnp-5883	379	15	of	of	ADP
fnp-5883	379	16	storage	storage	NOUN
fnp-5883	379	17	of	of	ADP
fnp-5883	379	18	brain	brain	NOUN
fnp-5883	379	19	tissue	tissue	NOUN
fnp-5883	379	20	at	at	ADP
fnp-5883	379	21	‑80	‑80	NOUN
fnp-5883	379	22	 	 	SPACE
fnp-5883	379	23	°	°	ADP
fnp-5883	379	24	c	c	NOUN
fnp-5883	379	25	(	(	PUNCT
fnp-5883	379	26	zilles	zille	NOUN
fnp-5883	379	27	et	et	PROPN
fnp-5883	379	28	al	al	PROPN
fnp-5883	379	29	.	.	PROPN
fnp-5883	379	30	,	,	PUNCT
fnp-5883	379	31	2002	2002	NUM
fnp-5883	379	32	;	;	PUNCT
fnp-5883	379	33	andreasson	andreasson	NOUN
fnp-5883	379	34	et	et	PROPN
fnp-5883	379	35	al	al	PROPN
fnp-5883	379	36	.	.	PROPN
fnp-5883	379	37	,	,	PUNCT
fnp-5883	379	38	2013	2013	NUM
fnp-5883	379	39	)	)	PUNCT
fnp-5883	379	40	.	.	PUNCT
fnp-5883	380	1	taken	take	VERB
fnp-5883	380	2	together	together	ADV
fnp-5883	380	3	,	,	PUNCT
fnp-5883	380	4	these	these	DET
fnp-5883	380	5	findings	finding	NOUN
fnp-5883	380	6	underscore	underscore	VERB
fnp-5883	380	7	the	the	DET
fnp-5883	380	8	importance	importance	NOUN
fnp-5883	380	9	of	of	ADP
fnp-5883	380	10	considering	consider	VERB
fnp-5883	380	11	storage	storage	NOUN
fnp-5883	380	12	length	length	NOUN
fnp-5883	380	13	as	as	ADP
fnp-5883	380	14	a	a	DET
fnp-5883	380	15	potential	potential	ADJ
fnp-5883	380	16	confounding	confound	VERB
fnp-5883	380	17	variable	variable	NOUN
fnp-5883	380	18	when	when	SCONJ
fnp-5883	380	19	analyzing	analyze	VERB
fnp-5883	380	20	banked	banked	ADJ
fnp-5883	380	21	specimens	specimen	NOUN
fnp-5883	380	22	,	,	PUNCT
fnp-5883	380	23	although	although	SCONJ
fnp-5883	380	24	whether	whether	SCONJ
fnp-5883	380	25	it	it	PRON
fnp-5883	380	26	makes	make	VERB
fnp-5883	380	27	a	a	DET
fnp-5883	380	28	large	large	ADJ
fnp-5883	380	29	difference	difference	NOUN
fnp-5883	380	30	to	to	ADP
fnp-5883	380	31	the	the	DET
fnp-5883	380	32	morphology	morphology	NOUN
fnp-5883	380	33	in	in	ADP
fnp-5883	380	34	practice	practice	NOUN
fnp-5883	380	35	is	be	AUX
fnp-5883	380	36	still	still	ADV
fnp-5883	380	37	an	an	DET
fnp-5883	380	38	open	open	ADJ
fnp-5883	380	39	question	question	NOUN
fnp-5883	380	40	and	and	CCONJ
fnp-5883	380	41	likely	likely	ADV
fnp-5883	380	42	depends	depend	VERB
fnp-5883	380	43	on	on	ADP
fnp-5883	380	44	the	the	DET
fnp-5883	380	45	variable	variable	NOUN
fnp-5883	380	46	of	of	ADP
fnp-5883	380	47	interest	interest	NOUN
fnp-5883	380	48	.	.	PUNCT
fnp-5883	381	1	storage	storage	NOUN
fnp-5883	381	2	below	below	ADP
fnp-5883	381	3	the	the	DET
fnp-5883	381	4	glass	glass	NOUN
fnp-5883	381	5	transition	transition	NOUN
fnp-5883	381	6	temperature	temperature	NOUN
fnp-5883	381	7	,	,	PUNCT
fnp-5883	381	8	on	on	ADP
fnp-5883	381	9	the	the	DET
fnp-5883	381	10	other	other	ADJ
fnp-5883	381	11	hand	hand	NOUN
fnp-5883	381	12	,	,	PUNCT
fnp-5883	381	13	is	be	AUX
fnp-5883	381	14	expected	expect	VERB
fnp-5883	381	15	to	to	PART
fnp-5883	381	16	cause	cause	VERB
fnp-5883	381	17	fewer	few	ADJ
fnp-5883	381	18	changes	change	NOUN
fnp-5883	381	19	.	.	PUNCT
fnp-5883	382	1	for	for	ADP
fnp-5883	382	2	example	example	NOUN
fnp-5883	382	3	,	,	PUNCT
fnp-5883	382	4	one	one	NUM
fnp-5883	382	5	study	study	NOUN
fnp-5883	382	6	found	find	VERB
fnp-5883	382	7	that	that	SCONJ
fnp-5883	382	8	while	while	SCONJ
fnp-5883	382	9	liver	liver	NOUN
fnp-5883	382	10	cell	cell	NOUN
fnp-5883	382	11	spheroids	spheroid	NOUN
fnp-5883	382	12	stored	store	VERB
fnp-5883	382	13	at	at	ADP
fnp-5883	382	14	‑80	‑80	NOUN
fnp-5883	382	15	 	 	SPACE
fnp-5883	382	16	°	°	SCONJ
fnp-5883	382	17	c	c	PROPN
fnp-5883	382	18	had	have	VERB
fnp-5883	382	19	a	a	DET
fnp-5883	382	20	loss	loss	NOUN
fnp-5883	382	21	of	of	ADP
fnp-5883	382	22	viable	viable	ADJ
fnp-5883	382	23	cells	cell	NOUN
fnp-5883	382	24	starting	start	VERB
fnp-5883	382	25	at	at	ADP
fnp-5883	382	26	one	one	NUM
fnp-5883	382	27	month	month	NOUN
fnp-5883	382	28	and	and	CCONJ
fnp-5883	382	29	progressing	progress	VERB
fnp-5883	382	30	substantially	substantially	ADV
fnp-5883	382	31	up	up	ADP
fnp-5883	382	32	until	until	ADP
fnp-5883	382	33	one	one	NUM
fnp-5883	382	34	year	year	NOUN
fnp-5883	382	35	,	,	PUNCT
fnp-5883	382	36	storage	storage	NOUN
fnp-5883	382	37	in	in	ADP
fnp-5883	382	38	liquid	liquid	ADJ
fnp-5883	382	39	nitrogen	nitrogen	NOUN
fnp-5883	382	40	vapor	vapor	NOUN
fnp-5883	382	41	at	at	ADP
fnp-5883	382	42	approximately	approximately	ADV
fnp-5883	382	43	‑170	‑170	ADP
fnp-5883	382	44	 	 	SPACE
fnp-5883	382	45	°	°	NOUN
fnp-5883	382	46	c	c	NOUN
fnp-5883	382	47	led	lead	VERB
fnp-5883	382	48	to	to	ADP
fnp-5883	382	49	no	no	DET
fnp-5883	382	50	changes	change	NOUN
fnp-5883	382	51	in	in	ADP
fnp-5883	382	52	viability	viability	NOUN
fnp-5883	382	53	(	(	PUNCT
fnp-5883	382	54	massie	massie	PROPN
fnp-5883	382	55	et	et	PROPN
fnp-5883	382	56	al	al	PROPN
fnp-5883	382	57	.	.	PROPN
fnp-5883	382	58	,	,	PUNCT
fnp-5883	382	59	2013	2013	NUM
fnp-5883	382	60	)	)	PUNCT
fnp-5883	382	61	.	.	PUNCT
fnp-5883	383	1	another	another	DET
fnp-5883	383	2	source	source	NOUN
fnp-5883	383	3	using	use	VERB
fnp-5883	383	4	storage	storage	NOUN
fnp-5883	383	5	at	at	ADP
fnp-5883	383	6	‑180	‑180	PROPN
fnp-5883	383	7	 	 	SPACE
fnp-5883	383	8	°	°	NOUN
fnp-5883	383	9	c	c	PROPN
fnp-5883	383	10	found	find	VERB
fnp-5883	383	11	that	that	SCONJ
fnp-5883	383	12	morphology	morphology	NOUN
fnp-5883	383	13	did	do	AUX
fnp-5883	383	14	not	not	PART
fnp-5883	383	15	vary	vary	VERB
fnp-5883	383	16	over	over	ADP
fnp-5883	383	17	the	the	DET
fnp-5883	383	18	storage	storage	NOUN
fnp-5883	383	19	period	period	NOUN
fnp-5883	383	20	of	of	ADP
fnp-5883	383	21	a	a	DET
fnp-5883	383	22	few	few	ADJ
fnp-5883	383	23	days	day	NOUN
fnp-5883	383	24	to	to	PART
fnp-5883	383	25	up	up	ADP
fnp-5883	383	26	to	to	PART
fnp-5883	383	27	more	more	ADJ
fnp-5883	383	28	than	than	ADP
fnp-5883	383	29	5	5	NUM
fnp-5883	383	30	years	year	NOUN
fnp-5883	383	31	(	(	PUNCT
fnp-5883	383	32	seilhean	seilhean	PROPN
fnp-5883	383	33	et	et	PROPN
fnp-5883	383	34	al	al	PROPN
fnp-5883	383	35	.	.	PROPN
fnp-5883	383	36	,	,	PUNCT
fnp-5883	383	37	1996	1996	NUM
fnp-5883	383	38	)	)	PUNCT
fnp-5883	383	39	.	.	PUNCT
fnp-5883	384	1	however	however	ADV
fnp-5883	384	2	,	,	PUNCT
fnp-5883	384	3	as	as	SCONJ
fnp-5883	384	4	previously	previously	ADV
fnp-5883	384	5	discussed	discuss	VERB
fnp-5883	384	6	,	,	PUNCT
fnp-5883	384	7	storage	storage	NOUN
fnp-5883	384	8	at	at	ADP
fnp-5883	384	9	this	this	DET
fnp-5883	384	10	temperature	temperature	NOUN
fnp-5883	384	11	can	can	AUX
fnp-5883	384	12	cause	cause	VERB
fnp-5883	384	13	a	a	DET
fnp-5883	384	14	buildup	buildup	NOUN
fnp-5883	384	15	of	of	ADP
fnp-5883	384	16	fractures	fracture	NOUN
fnp-5883	384	17	(	(	PUNCT
fnp-5883	384	18	hunt	hunt	NOUN
fnp-5883	384	19	et	et	NOUN
fnp-5883	384	20	al	al	PROPN
fnp-5883	384	21	.	.	PROPN
fnp-5883	384	22	,	,	PUNCT
fnp-5883	384	23	1994	1994	NUM
fnp-5883	384	24	)	)	PUNCT
fnp-5883	384	25	.	.	PUNCT
fnp-5883	385	1	perhaps	perhaps	ADV
fnp-5883	385	2	more	more	ADV
fnp-5883	385	3	importantly	importantly	ADV
fnp-5883	385	4	,	,	PUNCT
fnp-5883	385	5	storage	storage	NOUN
fnp-5883	385	6	at	at	ADP
fnp-5883	385	7	temperatures	temperature	NOUN
fnp-5883	385	8	below	below	ADP
fnp-5883	385	9	‑80	‑80	NOUN
fnp-5883	385	10	 	 	SPACE
fnp-5883	385	11	°	°	ADP
fnp-5883	385	12	c	c	NOUN
fnp-5883	385	13	would	would	AUX
fnp-5883	385	14	incur	incur	VERB
fnp-5883	385	15	a	a	DET
fnp-5883	385	16	significant	significant	ADJ
fnp-5883	385	17	cost	cost	NOUN
fnp-5883	385	18	that	that	PRON
fnp-5883	385	19	may	may	AUX
fnp-5883	385	20	not	not	PART
fnp-5883	385	21	be	be	AUX
fnp-5883	385	22	justified	justify	VERB
fnp-5883	385	23	for	for	ADP
fnp-5883	385	24	many	many	ADJ
fnp-5883	385	25	brain	brain	NOUN
fnp-5883	385	26	banks	bank	NOUN
fnp-5883	385	27	.	.	PUNCT
fnp-5883	386	1	it	it	PRON
fnp-5883	386	2	is	be	AUX
fnp-5883	386	3	also	also	ADV
fnp-5883	386	4	worthwhile	worthwhile	ADJ
fnp-5883	386	5	to	to	PART
fnp-5883	386	6	point	point	VERB
fnp-5883	386	7	out	out	ADP
fnp-5883	386	8	that	that	SCONJ
fnp-5883	386	9	any	any	DET
fnp-5883	386	10	freeze	freeze	ADJ
fnp-5883	386	11	-	-	PUNCT
fnp-5883	386	12	thaw	thaw	NOUN
fnp-5883	386	13	cycle	cycle	NOUN
fnp-5883	386	14	is	be	AUX
fnp-5883	386	15	likely	likely	ADJ
fnp-5883	386	16	to	to	PART
fnp-5883	386	17	be	be	AUX
fnp-5883	386	18	extremely	extremely	ADV
fnp-5883	386	19	damaging	damaging	ADJ
fnp-5883	386	20	to	to	ADP
fnp-5883	386	21	tissue	tissue	NOUN
fnp-5883	386	22	morphology	morphology	NOUN
fnp-5883	386	23	.	.	PUNCT
fnp-5883	387	1	one	one	NUM
fnp-5883	387	2	study	study	NOUN
fnp-5883	387	3	found	find	VERB
fnp-5883	387	4	that	that	SCONJ
fnp-5883	387	5	a	a	DET
fnp-5883	387	6	freeze	freeze	ADJ
fnp-5883	387	7	-	-	PUNCT
fnp-5883	387	8	thaw	thaw	NOUN
fnp-5883	387	9	cycle	cycle	NOUN
fnp-5883	387	10	softened	soften	VERB
fnp-5883	387	11	brain	brain	NOUN
fnp-5883	387	12	tissue	tissue	NOUN
fnp-5883	387	13	to	to	ADP
fnp-5883	387	14	such	such	DET
fnp-5883	387	15	a	a	DET
fnp-5883	387	16	degree	degree	NOUN
fnp-5883	387	17	that	that	SCONJ
fnp-5883	387	18	it	it	PRON
fnp-5883	387	19	was	be	AUX
fnp-5883	387	20	too	too	ADV
fnp-5883	387	21	difficult	difficult	ADJ
fnp-5883	387	22	to	to	PART
fnp-5883	387	23	process	process	VERB
fnp-5883	387	24	it	it	PRON
fnp-5883	387	25	for	for	ADP
fnp-5883	387	26	histologic	histologic	ADJ
fnp-5883	387	27	study	study	NOUN
fnp-5883	387	28	at	at	ADP
fnp-5883	387	29	all	all	ADV
fnp-5883	387	30	(	(	PUNCT
fnp-5883	387	31	schäfer	schäfer	NOUN
fnp-5883	387	32	and	and	CCONJ
fnp-5883	387	33	kaufmann	kaufmann	PROPN
fnp-5883	387	34	,	,	PUNCT
fnp-5883	387	35	1999	1999	NUM
fnp-5883	387	36	)	)	PUNCT
fnp-5883	387	37	.	.	PUNCT
fnp-5883	388	1	the	the	DET
fnp-5883	388	2	authors	author	NOUN
fnp-5883	388	3	noted	note	VERB
fnp-5883	388	4	that	that	SCONJ
fnp-5883	388	5	this	this	DET
fnp-5883	388	6	phenomenon	phenomenon	NOUN
fnp-5883	388	7	was	be	AUX
fnp-5883	388	8	common	common	ADJ
fnp-5883	388	9	in	in	ADP
fnp-5883	388	10	tissues	tissue	NOUN
fnp-5883	388	11	like	like	ADP
fnp-5883	388	12	the	the	DET
fnp-5883	388	13	brain	brain	NOUN
fnp-5883	388	14	that	that	PRON
fnp-5883	388	15	have	have	VERB
fnp-5883	388	16	a	a	DET
fnp-5883	388	17	relatively	relatively	ADV
fnp-5883	388	18	high	high	ADJ
fnp-5883	388	19	extracellular	extracellular	ADJ
fnp-5883	388	20	water	water	NOUN
fnp-5883	388	21	content	content	NOUN
fnp-5883	388	22	and	and	CCONJ
fnp-5883	388	23	relatively	relatively	ADV
fnp-5883	388	24	weak	weak	ADJ
fnp-5883	388	25	physical	physical	ADJ
fnp-5883	388	26	connections	connection	NOUN
fnp-5883	388	27	between	between	ADP
fnp-5883	388	28	cells	cell	NOUN
fnp-5883	388	29	.	.	PUNCT
fnp-5883	389	1	this	this	PRON
fnp-5883	389	2	is	be	AUX
fnp-5883	389	3	one	one	NUM
fnp-5883	389	4	of	of	ADP
fnp-5883	389	5	the	the	DET
fnp-5883	389	6	reasons	reason	NOUN
fnp-5883	389	7	that	that	PRON
fnp-5883	389	8	brain	brain	NOUN
fnp-5883	389	9	banks	bank	NOUN
fnp-5883	389	10	typically	typically	ADV
fnp-5883	389	11	break	break	VERB
fnp-5883	389	12	up	up	ADP
fnp-5883	389	13	fresh	fresh	ADJ
fnp-5883	389	14	tissue	tissue	NOUN
fnp-5883	389	15	into	into	ADP
fnp-5883	389	16	smaller	small	ADJ
fnp-5883	389	17	blocks	block	NOUN
fnp-5883	389	18	prior	prior	ADV
fnp-5883	389	19	to	to	ADP
fnp-5883	389	20	the	the	DET
fnp-5883	389	21	initial	initial	ADJ
fnp-5883	389	22	freezing	freezing	ADJ
fnp-5883	389	23	procedure	procedure	NOUN
fnp-5883	389	24	(	(	PUNCT
fnp-5883	389	25	shepherd	shepherd	NOUN
fnp-5883	389	26	et	et	PROPN
fnp-5883	389	27	al	al	PROPN
fnp-5883	389	28	.	.	PROPN
fnp-5883	389	29	,	,	PUNCT
fnp-5883	389	30	2019	2019	NUM
fnp-5883	389	31	)	)	PUNCT
fnp-5883	389	32	.	.	PUNCT
fnp-5883	390	1	relationship	relationship	NOUN
fnp-5883	390	2	between	between	ADP
fnp-5883	390	3	ice	ice	NOUN
fnp-5883	390	4	crystal	crystal	NOUN
fnp-5883	390	5	formation	formation	NOUN
fnp-5883	390	6	and	and	CCONJ
fnp-5883	390	7	postmortem	postmortem	NOUN
fnp-5883	390	8	decomposition	decomposition	NOUN
fnp-5883	390	9	it	it	PRON
fnp-5883	390	10	is	be	AUX
fnp-5883	390	11	mechanistically	mechanistically	ADV
fnp-5883	390	12	plausible	plausible	ADJ
fnp-5883	390	13	that	that	SCONJ
fnp-5883	390	14	a	a	DET
fnp-5883	390	15	longer	long	ADJ
fnp-5883	390	16	postmortem	postmortem	NOUN
fnp-5883	390	17	interval	interval	NOUN
fnp-5883	390	18	could	could	AUX
fnp-5883	390	19	increase	increase	VERB
fnp-5883	390	20	ice	ice	NOUN
fnp-5883	390	21	artifacts	artifact	NOUN
fnp-5883	390	22	,	,	PUNCT
fnp-5883	390	23	for	for	ADP
fnp-5883	390	24	two	two	NUM
fnp-5883	390	25	reasons	reason	NOUN
fnp-5883	390	26	.	.	PUNCT
fnp-5883	391	1	first	first	ADV
fnp-5883	391	2	,	,	PUNCT
fnp-5883	391	3	the	the	DET
fnp-5883	391	4	levels	level	NOUN
fnp-5883	391	5	of	of	ADP
fnp-5883	391	6	free	free	ADJ
fnp-5883	391	7	water	water	NOUN
fnp-5883	391	8	have	have	AUX
fnp-5883	391	9	been	be	AUX
fnp-5883	391	10	found	find	VERB
fnp-5883	391	11	to	to	PART
fnp-5883	391	12	increase	increase	VERB
fnp-5883	391	13	in	in	ADP
fnp-5883	391	14	the	the	DET
fnp-5883	391	15	brain	brain	NOUN
fnp-5883	391	16	after	after	ADP
fnp-5883	391	17	death	death	NOUN
fnp-5883	391	18	(	(	PUNCT
fnp-5883	391	19	ansari	ansari	PROPN
fnp-5883	391	20	et	et	PROPN
fnp-5883	391	21	al	al	PROPN
fnp-5883	391	22	.	.	PROPN
fnp-5883	391	23	,	,	PUNCT
fnp-5883	391	24	1976	1976	NUM
fnp-5883	391	25	;	;	PUNCT
fnp-5883	391	26	leonard	leonard	PROPN
fnp-5883	391	27	et	et	PROPN
fnp-5883	391	28	al	al	PROPN
fnp-5883	391	29	.	.	PROPN
fnp-5883	391	30	,	,	PUNCT
fnp-5883	391	31	2016	2016	NUM
fnp-5883	391	32	)	)	PUNCT
fnp-5883	391	33	.	.	PUNCT
fnp-5883	392	1	the	the	DET
fnp-5883	392	2	increased	increase	VERB
fnp-5883	392	3	water	water	NOUN
fnp-5883	392	4	content	content	NOUN
fnp-5883	392	5	may	may	AUX
fnp-5883	392	6	make	make	VERB
fnp-5883	392	7	tissue	tissue	NOUN
fnp-5883	392	8	more	more	ADV
fnp-5883	392	9	susceptible	susceptible	ADJ
fnp-5883	392	10	to	to	ADP
fnp-5883	392	11	ice	ice	NOUN
fnp-5883	392	12	crystal	crystal	NOUN
fnp-5883	392	13	formation	formation	NOUN
fnp-5883	392	14	during	during	ADP
fnp-5883	392	15	the	the	DET
fnp-5883	392	16	freezing	freezing	ADJ
fnp-5883	392	17	process	process	NOUN
fnp-5883	392	18	.	.	PUNCT
fnp-5883	393	1	second	second	ADJ
fnp-5883	393	2	,	,	PUNCT
fnp-5883	393	3	the	the	DET
fnp-5883	393	4	molecular	molecular	ADJ
fnp-5883	393	5	links	link	NOUN
fnp-5883	393	6	maintaining	maintain	VERB
fnp-5883	393	7	tissue	tissue	NOUN
fnp-5883	393	8	structures	structure	NOUN
fnp-5883	393	9	break	break	VERB
fnp-5883	393	10	down	down	ADP
fnp-5883	393	11	during	during	ADP
fnp-5883	393	12	the	the	DET
fnp-5883	393	13	postmortem	postmortem	NOUN
fnp-5883	393	14	interval	interval	NOUN
fnp-5883	393	15	(	(	PUNCT
fnp-5883	393	16	krassner	krassner	NOUN
fnp-5883	393	17	et	et	PROPN
fnp-5883	393	18	al	al	PROPN
fnp-5883	393	19	.	.	PROPN
fnp-5883	393	20	,	,	PUNCT
fnp-5883	393	21	2023	2023	NUM
fnp-5883	393	22	)	)	PUNCT
fnp-5883	393	23	.	.	PUNCT
fnp-5883	394	1	this	this	PRON
fnp-5883	394	2	may	may	AUX
fnp-5883	394	3	render	render	VERB
fnp-5883	394	4	tissue	tissue	NOUN
fnp-5883	394	5	structures	structure	NOUN
fnp-5883	394	6	more	more	ADV
fnp-5883	394	7	vulnerable	vulnerable	ADJ
fnp-5883	394	8	to	to	ADP
fnp-5883	394	9	the	the	DET
fnp-5883	394	10	mechanical	mechanical	ADJ
fnp-5883	394	11	stresses	stress	NOUN
fnp-5883	394	12	induced	induce	VERB
fnp-5883	394	13	by	by	ADP
fnp-5883	394	14	ice	ice	NOUN
fnp-5883	394	15	crystal	crystal	NOUN
fnp-5883	394	16	growth	growth	NOUN
fnp-5883	394	17	.	.	PUNCT
fnp-5883	395	1	this	this	DET
fnp-5883	395	2	relationship	relationship	NOUN
fnp-5883	395	3	is	be	AUX
fnp-5883	395	4	also	also	ADV
fnp-5883	395	5	consistent	consistent	ADJ
fnp-5883	395	6	with	with	ADP
fnp-5883	395	7	the	the	DET
fnp-5883	395	8	findings	finding	NOUN
fnp-5883	395	9	in	in	ADP
fnp-5883	395	10	frozen	frozen	ADJ
fnp-5883	395	11	sections	section	NOUN
fnp-5883	395	12	that	that	PRON
fnp-5883	395	13	tissue	tissue	NOUN
fnp-5883	395	14	edema	edema	NOUN
fnp-5883	395	15	tends	tend	VERB
fnp-5883	395	16	to	to	PART
fnp-5883	395	17	worsen	worsen	VERB
fnp-5883	395	18	ice	ice	NOUN
fnp-5883	395	19	artifacts	artifact	NOUN
fnp-5883	395	20	(	(	PUNCT
fnp-5883	395	21	tofte	tofte	PROPN
fnp-5883	395	22	et	et	NOUN
fnp-5883	395	23	al	al	PROPN
fnp-5883	395	24	.	.	PROPN
fnp-5883	395	25	,	,	PUNCT
fnp-5883	395	26	2014	2014	NUM
fnp-5883	395	27	;	;	PUNCT
fnp-5883	395	28	priemer	priemer	PROPN
fnp-5883	395	29	et	et	PROPN
fnp-5883	395	30	al	al	PROPN
fnp-5883	395	31	.	.	PROPN
fnp-5883	395	32	,	,	PUNCT
fnp-5883	395	33	2023	2023	NUM
fnp-5883	395	34	)	)	PUNCT
fnp-5883	395	35	.	.	PUNCT
fnp-5883	396	1	notably	notably	ADV
fnp-5883	396	2	,	,	PUNCT
fnp-5883	396	3	astrocytes	astrocyte	NOUN
fnp-5883	396	4	tend	tend	VERB
fnp-5883	396	5	to	to	PART
fnp-5883	396	6	accumulate	accumulate	VERB
fnp-5883	396	7	water	water	NOUN
fnp-5883	396	8	in	in	ADP
fnp-5883	396	9	the	the	DET
fnp-5883	396	10	postmortem	postmortem	NOUN
fnp-5883	396	11	interval	interval	NOUN
fnp-5883	396	12	,	,	PUNCT
fnp-5883	396	13	and	and	CCONJ
fnp-5883	396	14	electron	electron	NOUN
fnp-5883	396	15	microscopy	microscopy	NOUN
fnp-5883	396	16	has	have	AUX
fnp-5883	396	17	found	find	VERB
fnp-5883	396	18	that	that	DET
fnp-5883	396	19	astrocyte	astrocyte	NOUN
fnp-5883	396	20	membranes	membrane	NOUN
fnp-5883	396	21	are	be	AUX
fnp-5883	396	22	the	the	DET
fnp-5883	396	23	most	most	ADV
fnp-5883	396	24	vulnerable	vulnerable	ADJ
fnp-5883	396	25	to	to	ADP
fnp-5883	396	26	freezing	freeze	VERB
fnp-5883	396	27	damage	damage	NOUN
fnp-5883	396	28	,	,	PUNCT
fnp-5883	396	29	which	which	PRON
fnp-5883	396	30	is	be	AUX
fnp-5883	396	31	particularly	particularly	ADV
fnp-5883	396	32	perceptible	perceptible	ADJ
fnp-5883	396	33	around	around	ADP
fnp-5883	396	34	neurons	neuron	NOUN
fnp-5883	396	35	,	,	PUNCT
fnp-5883	396	36	oligodendrocytes	oligodendrocyte	NOUN
fnp-5883	396	37	,	,	PUNCT
fnp-5883	396	38	and	and	CCONJ
fnp-5883	396	39	capillaries	capillary	NOUN
fnp-5883	396	40	(	(	PUNCT
fnp-5883	396	41	walder	walder	NOUN
fnp-5883	396	42	and	and	CCONJ
fnp-5883	396	43	vrensen	vrensen	NOUN
fnp-5883	396	44	,	,	PUNCT
fnp-5883	396	45	1972	1972	NUM
fnp-5883	396	46	)	)	PUNCT
fnp-5883	396	47	.	.	PUNCT
fnp-5883	397	1	one	one	NUM
fnp-5883	397	2	study	study	NOUN
fnp-5883	397	3	found	find	VERB
fnp-5883	397	4	that	that	SCONJ
fnp-5883	397	5	the	the	DET
fnp-5883	397	6	method	method	NOUN
fnp-5883	397	7	of	of	ADP
fnp-5883	397	8	tissue	tissue	NOUN
fnp-5883	397	9	preservation	preservation	NOUN
fnp-5883	397	10	affected	affect	VERB
fnp-5883	397	11	how	how	SCONJ
fnp-5883	397	12	a	a	DET
fnp-5883	397	13	20	20	NUM
fnp-5883	397	14	hour	hour	NOUN
fnp-5883	397	15	postmortem	postmortem	NOUN
fnp-5883	397	16	delay	delay	NOUN
fnp-5883	397	17	affects	affect	VERB
fnp-5883	397	18	myelin	myelin	NOUN
fnp-5883	397	19	basic	basic	ADJ
fnp-5883	397	20	protein	protein	NOUN
fnp-5883	397	21	immunostaining	immunostaine	VERB
fnp-5883	397	22	(	(	PUNCT
fnp-5883	397	23	itoyama	itoyama	PROPN
fnp-5883	397	24	et	et	PROPN
fnp-5883	397	25	al	al	PROPN
fnp-5883	397	26	.	.	PROPN
fnp-5883	397	27	,	,	PUNCT
fnp-5883	397	28	1980	1980	NUM
fnp-5883	397	29	)	)	PUNCT
fnp-5883	397	30	.	.	PUNCT
fnp-5883	398	1	when	when	SCONJ
fnp-5883	398	2	using	use	VERB
fnp-5883	398	3	immersion	immersion	NOUN
fnp-5883	398	4	fixation	fixation	NOUN
fnp-5883	398	5	,	,	PUNCT
fnp-5883	398	6	the	the	DET
fnp-5883	398	7	authors	author	NOUN
fnp-5883	398	8	found	find	VERB
fnp-5883	398	9	that	that	SCONJ
fnp-5883	398	10	some	some	DET
fnp-5883	398	11	myelin	myelin	NOUN
fnp-5883	398	12	sheaths	sheath	NOUN
fnp-5883	398	13	became	became	AUX
fnp-5883	398	14	vacuolated	vacuolate	VERB
fnp-5883	398	15	after	after	ADP
fnp-5883	398	16	this	this	DET
fnp-5883	398	17	postmortem	postmortem	NOUN
fnp-5883	398	18	delay	delay	NOUN
fnp-5883	398	19	,	,	PUNCT
fnp-5883	398	20	but	but	CCONJ
fnp-5883	398	21	the	the	DET
fnp-5883	398	22	overall	overall	ADJ
fnp-5883	398	23	preservation	preservation	NOUN
fnp-5883	398	24	was	be	AUX
fnp-5883	398	25	satisfactory	satisfactory	ADJ
fnp-5883	398	26	.	.	PUNCT
fnp-5883	399	1	on	on	ADP
fnp-5883	399	2	the	the	DET
fnp-5883	399	3	other	other	ADJ
fnp-5883	399	4	hand	hand	NOUN
fnp-5883	399	5	,	,	PUNCT
fnp-5883	399	6	tissue	tissue	NOUN
fnp-5883	399	7	that	that	PRON
fnp-5883	399	8	was	be	AUX
fnp-5883	399	9	frozen	freeze	VERB
fnp-5883	399	10	after	after	SCONJ
fnp-5883	399	11	the	the	DET
fnp-5883	399	12	same	same	ADJ
fnp-5883	399	13	postmortem	postmortem	NOUN
fnp-5883	399	14	delay	delay	NOUN
fnp-5883	399	15	showed	show	VERB
fnp-5883	399	16	more	more	ADV
fnp-5883	399	17	severe	severe	ADJ
fnp-5883	399	18	changes	change	NOUN
fnp-5883	399	19	,	,	PUNCT
fnp-5883	399	20	including	include	VERB
fnp-5883	399	21	few	few	ADJ
fnp-5883	399	22	visible	visible	ADJ
fnp-5883	399	23	oligodendrocyte	oligodendrocyte	NOUN
fnp-5883	399	24	processes	process	NOUN
fnp-5883	399	25	and	and	CCONJ
fnp-5883	399	26	only	only	ADV
fnp-5883	399	27	faintly	faintly	ADV
fnp-5883	399	28	stained	stain	VERB
fnp-5883	399	29	myelin	myelin	ADJ
fnp-5883	399	30	sheaths	sheath	NOUN
fnp-5883	399	31	.	.	PUNCT
fnp-5883	400	1	although	although	SCONJ
fnp-5883	400	2	the	the	DET
fnp-5883	400	3	hypothesis	hypothesis	NOUN
fnp-5883	400	4	of	of	ADP
fnp-5883	400	5	a	a	DET
fnp-5883	400	6	relationship	relationship	NOUN
fnp-5883	400	7	between	between	ADP
fnp-5883	400	8	postmortem	postmortem	NOUN
fnp-5883	400	9	interval	interval	NOUN
fnp-5883	400	10	and	and	CCONJ
fnp-5883	400	11	ice	ice	NOUN
fnp-5883	400	12	damage	damage	NOUN
fnp-5883	400	13	is	be	AUX
fnp-5883	400	14	interesting	interesting	ADJ
fnp-5883	400	15	,	,	PUNCT
fnp-5883	400	16	it	it	PRON
fnp-5883	400	17	has	have	AUX
fnp-5883	400	18	not	not	PART
fnp-5883	400	19	been	be	AUX
fnp-5883	400	20	widely	widely	ADV
fnp-5883	400	21	investigated	investigate	VERB
fnp-5883	400	22	and	and	CCONJ
fnp-5883	400	23	it	it	PRON
fnp-5883	400	24	is	be	AUX
fnp-5883	400	25	worthy	worthy	ADJ
fnp-5883	400	26	of	of	ADP
fnp-5883	400	27	more	more	ADJ
fnp-5883	400	28	research	research	NOUN
fnp-5883	400	29	before	before	SCONJ
fnp-5883	400	30	too	too	ADV
fnp-5883	400	31	much	much	ADJ
fnp-5883	400	32	confidence	confidence	NOUN
fnp-5883	400	33	is	be	AUX
fnp-5883	400	34	placed	place	VERB
fnp-5883	400	35	into	into	ADP
fnp-5883	400	36	these	these	DET
fnp-5883	400	37	preliminary	preliminary	ADJ
fnp-5883	400	38	observations	observation	NOUN
fnp-5883	400	39	.	.	PUNCT
fnp-5883	401	1	another	another	DET
fnp-5883	401	2	relevant	relevant	ADJ
fnp-5883	401	3	factor	factor	NOUN
fnp-5883	401	4	is	be	AUX
fnp-5883	401	5	that	that	SCONJ
fnp-5883	401	6	some	some	DET
fnp-5883	401	7	structural	structural	ADJ
fnp-5883	401	8	changes	change	NOUN
fnp-5883	401	9	observed	observe	VERB
fnp-5883	401	10	in	in	ADP
fnp-5883	401	11	frozen	frozen	ADJ
fnp-5883	401	12	tissue	tissue	NOUN
fnp-5883	401	13	can	can	AUX
fnp-5883	401	14	overlap	overlap	VERB
fnp-5883	401	15	with	with	ADP
fnp-5883	401	16	those	those	PRON
fnp-5883	401	17	seen	see	VERB
fnp-5883	401	18	in	in	ADP
fnp-5883	401	19	postmortem	postmortem	NOUN
fnp-5883	401	20	autolysis	autolysis	NOUN
fnp-5883	401	21	.	.	PUNCT
fnp-5883	402	1	mechanistically	mechanistically	ADV
fnp-5883	402	2	,	,	PUNCT
fnp-5883	402	3	both	both	PRON
fnp-5883	402	4	freezing	freeze	VERB
fnp-5883	402	5	and	and	CCONJ
fnp-5883	402	6	postmortem	postmortem	NOUN
fnp-5883	402	7	decomposition	decomposition	NOUN
fnp-5883	402	8	would	would	AUX
fnp-5883	402	9	be	be	AUX
fnp-5883	402	10	expected	expect	VERB
fnp-5883	402	11	to	to	PART
fnp-5883	402	12	cause	cause	VERB
fnp-5883	402	13	cell	cell	NOUN
fnp-5883	402	14	membrane	membrane	NOUN
fnp-5883	402	15	damage	damage	NOUN
fnp-5883	402	16	.	.	PUNCT
fnp-5883	403	1	for	for	ADP
fnp-5883	403	2	example	example	NOUN
fnp-5883	403	3	,	,	PUNCT
fnp-5883	403	4	one	one	NUM
fnp-5883	403	5	study	study	NOUN
fnp-5883	403	6	noted	note	VERB
fnp-5883	403	7	that	that	SCONJ
fnp-5883	403	8	hemolysis	hemolysis	NOUN
fnp-5883	403	9	,	,	PUNCT
fnp-5883	403	10	characterized	characterize	VERB
fnp-5883	403	11	by	by	ADP
fnp-5883	403	12	the	the	DET
fnp-5883	403	13	loss	loss	NOUN
fnp-5883	403	14	of	of	ADP
fnp-5883	403	15	erythrocyte	erythrocyte	NOUN
fnp-5883	403	16	cytoplasm	cytoplasm	NOUN
fnp-5883	403	17	,	,	PUNCT
fnp-5883	403	18	was	be	AUX
fnp-5883	403	19	present	present	ADJ
fnp-5883	403	20	in	in	ADP
fnp-5883	403	21	both	both	DET
fnp-5883	403	22	frozen	frozen	ADJ
fnp-5883	403	23	-	-	PUNCT
fnp-5883	403	24	thawed	thaw	VERB
fnp-5883	403	25	and	and	CCONJ
fnp-5883	403	26	decomposed	decompose	VERB
fnp-5883	403	27	fish	fish	NOUN
fnp-5883	403	28	brain	brain	NOUN
fnp-5883	403	29	tissue	tissue	NOUN
fnp-5883	403	30	(	(	PUNCT
fnp-5883	403	31	kagan	kagan	NOUN
fnp-5883	403	32	and	and	CCONJ
fnp-5883	403	33	viner	viner	NOUN
fnp-5883	403	34	,	,	PUNCT
fnp-5883	403	35	2022	2022	NUM
fnp-5883	403	36	)	)	PUNCT
fnp-5883	403	37	.	.	PUNCT
fnp-5883	404	1	another	another	DET
fnp-5883	404	2	study	study	NOUN
fnp-5883	404	3	,	,	PUNCT
fnp-5883	404	4	comparing	compare	VERB
fnp-5883	404	5	the	the	DET
fnp-5883	404	6	ultrastructural	ultrastructural	ADJ
fnp-5883	404	7	alterations	alteration	NOUN
fnp-5883	404	8	in	in	ADP
fnp-5883	404	9	larval	larval	ADJ
fnp-5883	404	10	fly	fly	NOUN
fnp-5883	404	11	brains	brain	NOUN
fnp-5883	404	12	exposed	expose	VERB
fnp-5883	404	13	to	to	ADP
fnp-5883	404	14	either	either	CCONJ
fnp-5883	404	15	lethal	lethal	ADJ
fnp-5883	404	16	freezing	freezing	NOUN
fnp-5883	404	17	or	or	CCONJ
fnp-5883	404	18	anoxia	anoxia	NOUN
fnp-5883	404	19	,	,	PUNCT
fnp-5883	404	20	found	find	VERB
fnp-5883	404	21	similarities	similarity	NOUN
fnp-5883	404	22	such	such	ADJ
fnp-5883	404	23	as	as	ADP
fnp-5883	404	24	nuclear	nuclear	ADJ
fnp-5883	404	25	membrane	membrane	NOUN
fnp-5883	404	26	expansions	expansion	NOUN
fnp-5883	404	27	,	,	PUNCT
fnp-5883	404	28	nuclear	nuclear	ADJ
fnp-5883	404	29	chromatin	chromatin	NOUN
fnp-5883	404	30	clumping	clumping	NOUN
fnp-5883	404	31	,	,	PUNCT
fnp-5883	404	32	and	and	CCONJ
fnp-5883	404	33	rough	rough	ADJ
fnp-5883	404	34	endoplasmic	endoplasmic	ADJ
fnp-5883	404	35	reticulum	reticulum	NOUN
fnp-5883	404	36	swelling	swell	VERB
fnp-5883	404	37	(	(	PUNCT
fnp-5883	404	38	lee	lee	PROPN
fnp-5883	404	39	et	et	PROPN
fnp-5883	404	40	al	al	PROPN
fnp-5883	404	41	.	.	PROPN
fnp-5883	404	42	,	,	PUNCT
fnp-5883	404	43	1997	1997	NUM
fnp-5883	404	44	)	)	PUNCT
fnp-5883	404	45	.	.	PUNCT
fnp-5883	405	1	these	these	DET
fnp-5883	405	2	findings	finding	NOUN
fnp-5883	405	3	suggest	suggest	VERB
fnp-5883	405	4	that	that	SCONJ
fnp-5883	405	5	some	some	PRON
fnp-5883	405	6	of	of	ADP
fnp-5883	405	7	the	the	DET
fnp-5883	405	8	damage	damage	NOUN
fnp-5883	405	9	observed	observe	VERB
fnp-5883	405	10	in	in	ADP
fnp-5883	405	11	frozen	frozen	ADJ
fnp-5883	405	12	brain	brain	NOUN
fnp-5883	405	13	tissue	tissue	NOUN
fnp-5883	405	14	may	may	AUX
fnp-5883	405	15	be	be	AUX
fnp-5883	405	16	exacerbated	exacerbate	VERB
fnp-5883	405	17	by	by	ADP
fnp-5883	405	18	–	–	PUNCT
fnp-5883	405	19	or	or	CCONJ
fnp-5883	405	20	difficult	difficult	ADJ
fnp-5883	405	21	to	to	PART
fnp-5883	405	22	distinguish	distinguish	VERB
fnp-5883	405	23	from	from	ADP
fnp-5883	405	24	–	–	PUNCT
fnp-5883	405	25	the	the	DET
fnp-5883	405	26	changes	change	NOUN
fnp-5883	405	27	that	that	PRON
fnp-5883	405	28	occur	occur	VERB
fnp-5883	405	29	during	during	ADP
fnp-5883	405	30	the	the	DET
fnp-5883	405	31	postmortem	postmortem	NOUN
fnp-5883	405	32	interval	interval	NOUN
fnp-5883	405	33	.	.	PUNCT
fnp-5883	406	1	effects	effect	NOUN
fnp-5883	406	2	of	of	ADP
fnp-5883	406	3	cryopreservation	cryopreservation	NOUN
fnp-5883	406	4	on	on	ADP
fnp-5883	406	5	biomolecular	biomolecular	ADJ
fnp-5883	406	6	preservation	preservation	NOUN
fnp-5883	406	7	modern	modern	ADJ
fnp-5883	406	8	brain	brain	NOUN
fnp-5883	406	9	banking	banking	NOUN
fnp-5883	406	10	aims	aim	VERB
fnp-5883	406	11	to	to	PART
fnp-5883	406	12	preserve	preserve	VERB
fnp-5883	406	13	multiple	multiple	ADJ
fnp-5883	406	14	tissue	tissue	NOUN
fnp-5883	406	15	properties	property	NOUN
fnp-5883	406	16	,	,	PUNCT
fnp-5883	406	17	and	and	CCONJ
fnp-5883	406	18	many	many	ADJ
fnp-5883	406	19	tissue	tissue	NOUN
fnp-5883	406	20	requests	request	NOUN
fnp-5883	406	21	are	be	AUX
fnp-5883	406	22	for	for	ADP
fnp-5883	406	23	molecular	molecular	ADJ
fnp-5883	406	24	analyses	analysis	NOUN
fnp-5883	406	25	,	,	PUNCT
fnp-5883	406	26	making	make	VERB
fnp-5883	406	27	this	this	DET
fnp-5883	406	28	aspect	aspect	NOUN
fnp-5883	406	29	of	of	ADP
fnp-5883	406	30	preservation	preservation	NOUN
fnp-5883	406	31	quality	quality	NOUN
fnp-5883	406	32	also	also	ADV
fnp-5883	406	33	critical	critical	ADJ
fnp-5883	406	34	to	to	PART
fnp-5883	406	35	consider	consider	VERB
fnp-5883	406	36	.	.	PUNCT
fnp-5883	407	1	from	from	ADP
fnp-5883	407	2	a	a	DET
fnp-5883	407	3	mechanistic	mechanistic	ADJ
fnp-5883	407	4	perspective	perspective	NOUN
fnp-5883	407	5	,	,	PUNCT
fnp-5883	407	6	cryopreservation	cryopreservation	NOUN
fnp-5883	407	7	is	be	AUX
fnp-5883	407	8	not	not	PART
fnp-5883	407	9	thought	think	VERB
fnp-5883	407	10	to	to	PART
fnp-5883	407	11	cause	cause	VERB
fnp-5883	407	12	the	the	DET
fnp-5883	407	13	formation	formation	NOUN
fnp-5883	407	14	or	or	CCONJ
fnp-5883	407	15	breakdown	breakdown	NOUN
fnp-5883	407	16	of	of	ADP
fnp-5883	407	17	covalent	covalent	ADJ
fnp-5883	407	18	bonds	bond	NOUN
fnp-5883	407	19	in	in	ADP
fnp-5883	407	20	biomolecules	biomolecule	NOUN
fnp-5883	407	21	(	(	PUNCT
fnp-5883	407	22	fahy	fahy	PROPN
fnp-5883	407	23	and	and	CCONJ
fnp-5883	407	24	wowk	wowk	PROPN
fnp-5883	407	25	,	,	PUNCT
fnp-5883	407	26	2015	2015	NUM
fnp-5883	407	27	)	)	PUNCT
fnp-5883	407	28	,	,	PUNCT
fnp-5883	407	29	which	which	PRON
fnp-5883	407	30	is	be	AUX
fnp-5883	407	31	an	an	DET
fnp-5883	407	32	obvious	obvious	ADJ
fnp-5883	407	33	advantage	advantage	NOUN
fnp-5883	407	34	over	over	ADP
fnp-5883	407	35	chemical	chemical	ADJ
fnp-5883	407	36	fixation	fixation	NOUN
fnp-5883	407	37	by	by	ADP
fnp-5883	407	38	aldehydes	aldehyde	NOUN
fnp-5883	407	39	for	for	ADP
fnp-5883	407	40	the	the	DET
fnp-5883	407	41	purpose	purpose	NOUN
fnp-5883	407	42	of	of	ADP
fnp-5883	407	43	biomolecular	biomolecular	ADJ
fnp-5883	407	44	assays	assay	NOUN
fnp-5883	407	45	.	.	PUNCT
fnp-5883	408	1	however	however	ADV
fnp-5883	408	2	,	,	PUNCT
fnp-5883	408	3	cryopreservation	cryopreservation	NOUN
fnp-5883	408	4	can	can	AUX
fnp-5883	408	5	cause	cause	VERB
fnp-5883	408	6	numerous	numerous	ADJ
fnp-5883	408	7	other	other	ADJ
fnp-5883	408	8	effects	effect	NOUN
fnp-5883	408	9	on	on	ADP
fnp-5883	408	10	biomolecules	biomolecule	NOUN
fnp-5883	408	11	,	,	PUNCT
fnp-5883	408	12	including	include	VERB
fnp-5883	408	13	phase	phase	NOUN
fnp-5883	408	14	changes	change	NOUN
fnp-5883	408	15	in	in	ADP
fnp-5883	408	16	membrane	membrane	NOUN
fnp-5883	408	17	lipids	lipid	NOUN
fnp-5883	408	18	,	,	PUNCT
fnp-5883	408	19	conformational	conformational	ADJ
fnp-5883	408	20	changes	change	NOUN
fnp-5883	408	21	due	due	ADP
fnp-5883	408	22	to	to	ADP
fnp-5883	408	23	loss	loss	NOUN
fnp-5883	408	24	of	of	ADP
fnp-5883	408	25	the	the	DET
fnp-5883	408	26	hydration	hydration	NOUN
fnp-5883	408	27	shell	shell	NOUN
fnp-5883	408	28	,	,	PUNCT
fnp-5883	408	29	hydrophobic	hydrophobic	NOUN
fnp-5883	408	30	aggregation	aggregation	NOUN
fnp-5883	408	31	of	of	ADP
fnp-5883	408	32	biomolecules	biomolecule	NOUN
fnp-5883	408	33	,	,	PUNCT
fnp-5883	408	34	and	and	CCONJ
fnp-5883	408	35	protein	protein	NOUN
fnp-5883	408	36	inactivation	inactivation	NOUN
fnp-5883	408	37	,	,	PUNCT
fnp-5883	408	38	for	for	ADP
fnp-5883	408	39	example	example	NOUN
fnp-5883	408	40	of	of	ADP
fnp-5883	408	41	the	the	DET
fnp-5883	408	42	sodium	sodium	NOUN
fnp-5883	408	43	–	–	PUNCT
fnp-5883	408	44	potassium	potassium	NOUN
fnp-5883	408	45	pump	pump	NOUN
fnp-5883	408	46	(	(	PUNCT
fnp-5883	408	47	jain	jain	PROPN
fnp-5883	408	48	et	et	PROPN
fnp-5883	408	49	al	al	PROPN
fnp-5883	408	50	.	.	PROPN
fnp-5883	408	51	,	,	PUNCT
fnp-5883	408	52	2021	2021	NUM
fnp-5883	408	53	;	;	PUNCT
fnp-5883	408	54	pegg	pegg	NOUN
fnp-5883	408	55	,	,	PUNCT
fnp-5883	408	56	1976	1976	NUM
fnp-5883	408	57	)	)	PUNCT
fnp-5883	408	58	.	.	PUNCT
fnp-5883	409	1	some	some	DET
fnp-5883	409	2	biomolecular	biomolecular	ADJ
fnp-5883	409	3	changes	change	NOUN
fnp-5883	409	4	occur	occur	VERB
fnp-5883	409	5	during	during	ADP
fnp-5883	409	6	the	the	DET
fnp-5883	409	7	cryopreservation	cryopreservation	NOUN
fnp-5883	409	8	process	process	NOUN
fnp-5883	409	9	because	because	SCONJ
fnp-5883	409	10	metabolism	metabolism	NOUN
fnp-5883	409	11	does	do	AUX
fnp-5883	409	12	not	not	PART
fnp-5883	409	13	fully	fully	ADV
fnp-5883	409	14	stop	stop	VERB
fnp-5883	409	15	until	until	SCONJ
fnp-5883	409	16	very	very	ADV
fnp-5883	409	17	low	low	ADJ
fnp-5883	409	18	temperatures	temperature	NOUN
fnp-5883	409	19	are	be	AUX
fnp-5883	409	20	reached	reach	VERB
fnp-5883	409	21	.	.	PUNCT
fnp-5883	410	1	for	for	ADP
fnp-5883	410	2	this	this	DET
fnp-5883	410	3	reason	reason	NOUN
fnp-5883	410	4	,	,	PUNCT
fnp-5883	410	5	faster	fast	ADV
fnp-5883	410	6	cooling	cool	VERB
fnp-5883	410	7	processes	process	NOUN
fnp-5883	410	8	might	might	AUX
fnp-5883	410	9	be	be	AUX
fnp-5883	410	10	expected	expect	VERB
fnp-5883	410	11	to	to	PART
fnp-5883	410	12	yield	yield	VERB
fnp-5883	410	13	superior	superior	ADJ
fnp-5883	410	14	biomolecular	biomolecular	ADJ
fnp-5883	410	15	preservation	preservation	NOUN
fnp-5883	410	16	.	.	PUNCT
fnp-5883	411	1	indeed	indeed	ADV
fnp-5883	411	2	,	,	PUNCT
fnp-5883	411	3	for	for	ADP
fnp-5883	411	4	tumor	tumor	NOUN
fnp-5883	411	5	tissues	tissue	NOUN
fnp-5883	411	6	,	,	PUNCT
fnp-5883	411	7	snap	snap	VERB
fnp-5883	411	8	freezing	freezing	NOUN
fnp-5883	411	9	of	of	ADP
fnp-5883	411	10	tumor	tumor	NOUN
fnp-5883	411	11	biopsies	biopsy	NOUN
fnp-5883	411	12	has	have	AUX
fnp-5883	411	13	been	be	AUX
fnp-5883	411	14	reported	report	VERB
fnp-5883	411	15	to	to	PART
fnp-5883	411	16	be	be	AUX
fnp-5883	411	17	the	the	DET
fnp-5883	411	18	gold	gold	ADJ
fnp-5883	411	19	standard	standard	NOUN
fnp-5883	411	20	for	for	ADP
fnp-5883	411	21	maintenance	maintenance	NOUN
fnp-5883	411	22	of	of	ADP
fnp-5883	411	23	biomolecular	biomolecular	ADJ
fnp-5883	411	24	properties	property	NOUN
fnp-5883	411	25	of	of	ADP
fnp-5883	411	26	tissues	tissue	NOUN
fnp-5883	411	27	,	,	PUNCT
fnp-5883	411	28	such	such	ADJ
fnp-5883	411	29	as	as	ADP
fnp-5883	411	30	their	their	PRON
fnp-5883	411	31	content	content	NOUN
fnp-5883	411	32	of	of	ADP
fnp-5883	411	33	nucleic	nucleic	ADJ
fnp-5883	411	34	acids	acid	NOUN
fnp-5883	411	35	and	and	CCONJ
fnp-5883	411	36	phosphoproteins	phosphoprotein	NOUN
fnp-5883	411	37	(	(	PUNCT
fnp-5883	411	38	van	van	PROPN
fnp-5883	411	39	der	der	NOUN
fnp-5883	411	40	wijngaart	wijngaart	VERB
fnp-5883	411	41	et	et	PROPN
fnp-5883	411	42	al	al	PROPN
fnp-5883	411	43	.	.	PROPN
fnp-5883	411	44	,	,	PUNCT
fnp-5883	411	45	2023	2023	NUM
fnp-5883	411	46	)	)	PUNCT
fnp-5883	411	47	.	.	PUNCT
fnp-5883	412	1	however	however	ADV
fnp-5883	412	2	,	,	PUNCT
fnp-5883	412	3	other	other	ADJ
fnp-5883	412	4	data	datum	NOUN
fnp-5883	412	5	have	have	AUX
fnp-5883	412	6	suggested	suggest	VERB
fnp-5883	412	7	that	that	SCONJ
fnp-5883	412	8	slow	slow	ADJ
fnp-5883	412	9	freezing	freezing	NOUN
fnp-5883	412	10	may	may	AUX
fnp-5883	412	11	be	be	AUX
fnp-5883	412	12	preferable	preferable	ADJ
fnp-5883	412	13	for	for	ADP
fnp-5883	412	14	maintaining	maintain	VERB
fnp-5883	412	15	biomolecular	biomolecular	ADJ
fnp-5883	412	16	properties	property	NOUN
fnp-5883	412	17	,	,	PUNCT
fnp-5883	412	18	because	because	SCONJ
fnp-5883	412	19	fast	fast	ADJ
fnp-5883	412	20	freezing	freezing	NOUN
fnp-5883	412	21	yields	yield	VERB
fnp-5883	412	22	small	small	ADJ
fnp-5883	412	23	ice	ice	NOUN
fnp-5883	412	24	crystals	crystal	NOUN
fnp-5883	412	25	with	with	ADP
fnp-5883	412	26	an	an	DET
fnp-5883	412	27	increased	increase	VERB
fnp-5883	412	28	contact	contact	NOUN
fnp-5883	412	29	area	area	NOUN
fnp-5883	412	30	between	between	ADP
fnp-5883	412	31	ice	ice	NOUN
fnp-5883	412	32	and	and	CCONJ
fnp-5883	412	33	liquid	liquid	NOUN
fnp-5883	412	34	,	,	PUNCT
fnp-5883	412	35	which	which	PRON
fnp-5883	412	36	may	may	AUX
fnp-5883	412	37	increase	increase	VERB
fnp-5883	412	38	the	the	DET
fnp-5883	412	39	net	net	ADJ
fnp-5883	412	40	exposure	exposure	NOUN
fnp-5883	412	41	of	of	ADP
fnp-5883	412	42	proteins	protein	NOUN
fnp-5883	412	43	to	to	ADP
fnp-5883	412	44	the	the	DET
fnp-5883	412	45	damaging	damaging	ADJ
fnp-5883	412	46	effects	effect	NOUN
fnp-5883	412	47	of	of	ADP
fnp-5883	412	48	ice	ice	NOUN
fnp-5883	412	49	(	(	PUNCT
fnp-5883	412	50	cao	cao	PROPN
fnp-5883	412	51	et	et	PROPN
fnp-5883	412	52	al	al	PROPN
fnp-5883	412	53	.	.	PROPN
fnp-5883	412	54	,	,	PUNCT
fnp-5883	412	55	2003	2003	NUM
fnp-5883	412	56	)	)	PUNCT
fnp-5883	412	57	.	.	PUNCT
fnp-5883	413	1	one	one	NUM
fnp-5883	413	2	potential	potential	ADJ
fnp-5883	413	3	solution	solution	NOUN
fnp-5883	413	4	to	to	ADP
fnp-5883	413	5	the	the	DET
fnp-5883	413	6	problem	problem	NOUN
fnp-5883	413	7	of	of	ADP
fnp-5883	413	8	biomolecular	biomolecular	ADJ
fnp-5883	413	9	damage	damage	NOUN
fnp-5883	413	10	due	due	ADP
fnp-5883	413	11	to	to	ADP
fnp-5883	413	12	ice	ice	NOUN
fnp-5883	413	13	formation	formation	NOUN
fnp-5883	413	14	would	would	AUX
fnp-5883	413	15	be	be	AUX
fnp-5883	413	16	to	to	PART
fnp-5883	413	17	use	use	VERB
fnp-5883	413	18	cryoprotectants	cryoprotectant	NOUN
fnp-5883	413	19	,	,	PUNCT
fnp-5883	413	20	since	since	SCONJ
fnp-5883	413	21	a	a	DET
fnp-5883	413	22	sufficient	sufficient	ADJ
fnp-5883	413	23	concentration	concentration	NOUN
fnp-5883	413	24	of	of	ADP
fnp-5883	413	25	cryoprotectants	cryoprotectant	NOUN
fnp-5883	413	26	could	could	AUX
fnp-5883	413	27	prevent	prevent	VERB
fnp-5883	413	28	ice	ice	NOUN
fnp-5883	413	29	damage	damage	NOUN
fnp-5883	413	30	.	.	PUNCT
fnp-5883	414	1	cryoprotectants	cryoprotectant	NOUN
fnp-5883	414	2	could	could	AUX
fnp-5883	414	3	also	also	ADV
fnp-5883	414	4	allow	allow	VERB
fnp-5883	414	5	for	for	ADP
fnp-5883	414	6	better	well	ADJ
fnp-5883	414	7	maintenance	maintenance	NOUN
fnp-5883	414	8	of	of	ADP
fnp-5883	414	9	cellular	cellular	ADJ
fnp-5883	414	10	integrity	integrity	NOUN
fnp-5883	414	11	,	,	PUNCT
fnp-5883	414	12	which	which	PRON
fnp-5883	414	13	could	could	AUX
fnp-5883	414	14	be	be	AUX
fnp-5883	414	15	useful	useful	ADJ
fnp-5883	414	16	for	for	ADP
fnp-5883	414	17	the	the	DET
fnp-5883	414	18	molecular	molecular	ADJ
fnp-5883	414	19	profiling	profiling	NOUN
fnp-5883	414	20	of	of	ADP
fnp-5883	414	21	single	single	ADJ
fnp-5883	414	22	cells	cell	NOUN
fnp-5883	414	23	.	.	PUNCT
fnp-5883	415	1	however	however	ADV
fnp-5883	415	2	,	,	PUNCT
fnp-5883	415	3	if	if	SCONJ
fnp-5883	415	4	cryoprotectants	cryoprotectant	NOUN
fnp-5883	415	5	are	be	AUX
fnp-5883	415	6	used	use	VERB
fnp-5883	415	7	,	,	PUNCT
fnp-5883	415	8	they	they	PRON
fnp-5883	415	9	also	also	ADV
fnp-5883	415	10	have	have	VERB
fnp-5883	415	11	the	the	DET
fnp-5883	415	12	potential	potential	NOUN
fnp-5883	415	13	to	to	PART
fnp-5883	415	14	affect	affect	VERB
fnp-5883	415	15	the	the	DET
fnp-5883	415	16	molecular	molecular	ADJ
fnp-5883	415	17	properties	property	NOUN
fnp-5883	415	18	of	of	ADP
fnp-5883	415	19	the	the	DET
fnp-5883	415	20	tissue	tissue	NOUN
fnp-5883	415	21	itself	itself	PRON
fnp-5883	415	22	,	,	PUNCT
fnp-5883	415	23	for	for	ADP
fnp-5883	415	24	instance	instance	NOUN
fnp-5883	415	25	by	by	ADP
fnp-5883	415	26	causing	cause	VERB
fnp-5883	415	27	protein	protein	NOUN
fnp-5883	415	28	denaturation	denaturation	NOUN
fnp-5883	415	29	(	(	PUNCT
fnp-5883	415	30	fahy	fahy	PROPN
fnp-5883	415	31	and	and	CCONJ
fnp-5883	415	32	wowk	wowk	PROPN
fnp-5883	415	33	,	,	PUNCT
fnp-5883	415	34	2015	2015	NUM
fnp-5883	415	35	)	)	PUNCT
fnp-5883	415	36	.	.	PUNCT
fnp-5883	416	1	for	for	ADP
fnp-5883	416	2	some	some	DET
fnp-5883	416	3	molecular	molecular	ADJ
fnp-5883	416	4	assays	assay	NOUN
fnp-5883	416	5	,	,	PUNCT
fnp-5883	416	6	cryoprotectants	cryoprotectant	NOUN
fnp-5883	416	7	used	use	VERB
fnp-5883	416	8	at	at	ADP
fnp-5883	416	9	potentially	potentially	ADV
fnp-5883	416	10	variable	variable	ADJ
fnp-5883	416	11	concentrations	concentration	NOUN
fnp-5883	416	12	within	within	ADP
fnp-5883	416	13	or	or	CCONJ
fnp-5883	416	14	between	between	ADP
fnp-5883	416	15	samples	sample	NOUN
fnp-5883	416	16	could	could	AUX
fnp-5883	416	17	also	also	ADV
fnp-5883	416	18	create	create	VERB
fnp-5883	416	19	batch	batch	NOUN
fnp-5883	416	20	effects	effect	NOUN
fnp-5883	416	21	that	that	PRON
fnp-5883	416	22	would	would	AUX
fnp-5883	416	23	confound	confound	VERB
fnp-5883	416	24	subsequent	subsequent	ADJ
fnp-5883	416	25	analyses	analysis	NOUN
fnp-5883	416	26	.	.	PUNCT
fnp-5883	417	1	in	in	ADP
fnp-5883	417	2	practical	practical	ADJ
fnp-5883	417	3	brain	brain	NOUN
fnp-5883	417	4	banking	banking	NOUN
fnp-5883	417	5	settings	setting	NOUN
fnp-5883	417	6	,	,	PUNCT
fnp-5883	417	7	cryopreservation	cryopreservation	NOUN
fnp-5883	417	8	of	of	ADP
fnp-5883	417	9	small	small	ADJ
fnp-5883	417	10	tissue	tissue	NOUN
fnp-5883	417	11	blocks	block	NOUN
fnp-5883	417	12	in	in	ADP
fnp-5883	417	13	isopentane	isopentane	NOUN
fnp-5883	417	14	cooled	cool	VERB
fnp-5883	417	15	by	by	ADP
fnp-5883	417	16	liquid	liquid	ADJ
fnp-5883	417	17	nitrogen	nitrogen	NOUN
fnp-5883	417	18	has	have	AUX
fnp-5883	417	19	been	be	AUX
fnp-5883	417	20	reported	report	VERB
fnp-5883	417	21	to	to	PART
fnp-5883	417	22	be	be	AUX
fnp-5883	417	23	a	a	DET
fnp-5883	417	24	preferred	preferred	ADJ
fnp-5883	417	25	method	method	NOUN
fnp-5883	417	26	for	for	ADP
fnp-5883	417	27	obtaining	obtain	VERB
fnp-5883	417	28	high	high	ADJ
fnp-5883	417	29	-	-	PUNCT
fnp-5883	417	30	quality	quality	NOUN
fnp-5883	417	31	rna	rna	NOUN
fnp-5883	417	32	and	and	CCONJ
fnp-5883	417	33	protein	protein	NOUN
fnp-5883	417	34	preservation	preservation	NOUN
fnp-5883	417	35	(	(	PUNCT
fnp-5883	417	36	durrenberger	durrenberger	NOUN
fnp-5883	417	37	et	et	PROPN
fnp-5883	417	38	al	al	PROPN
fnp-5883	417	39	.	.	PROPN
fnp-5883	417	40	,	,	PUNCT
fnp-5883	417	41	2010	2010	NUM
fnp-5883	417	42	)	)	PUNCT
fnp-5883	417	43	.	.	PUNCT
fnp-5883	418	1	however	however	ADV
fnp-5883	418	2	,	,	PUNCT
fnp-5883	418	3	different	different	ADJ
fnp-5883	418	4	freezing	freezing	NOUN
fnp-5883	418	5	methods	method	NOUN
fnp-5883	418	6	have	have	AUX
fnp-5883	418	7	been	be	AUX
fnp-5883	418	8	found	find	VERB
fnp-5883	418	9	not	not	PART
fnp-5883	418	10	to	to	PART
fnp-5883	418	11	have	have	VERB
fnp-5883	418	12	a	a	DET
fnp-5883	418	13	significant	significant	ADJ
fnp-5883	418	14	effect	effect	NOUN
fnp-5883	418	15	on	on	ADP
fnp-5883	418	16	the	the	DET
fnp-5883	418	17	quality	quality	NOUN
fnp-5883	418	18	of	of	ADP
fnp-5883	418	19	rna	rna	NOUN
fnp-5883	418	20	harvested	harvest	VERB
fnp-5883	418	21	from	from	ADP
fnp-5883	418	22	brain	brain	NOUN
fnp-5883	418	23	tissue	tissue	NOUN
fnp-5883	418	24	(	(	PUNCT
fnp-5883	418	25	durrenberger	durrenberger	NOUN
fnp-5883	418	26	et	et	NOUN
fnp-5883	418	27	al	al	PROPN
fnp-5883	418	28	.	.	PROPN
fnp-5883	418	29	,	,	PUNCT
fnp-5883	418	30	2010	2010	NUM
fnp-5883	418	31	)	)	PUNCT
fnp-5883	418	32	.	.	PUNCT
fnp-5883	419	1	additionally	additionally	ADV
fnp-5883	419	2	,	,	PUNCT
fnp-5883	419	3	one	one	NUM
fnp-5883	419	4	study	study	NOUN
fnp-5883	419	5	found	find	VERB
fnp-5883	419	6	that	that	SCONJ
fnp-5883	419	7	there	there	PRON
fnp-5883	419	8	was	be	VERB
fnp-5883	419	9	no	no	DET
fnp-5883	419	10	difference	difference	NOUN
fnp-5883	419	11	between	between	ADP
fnp-5883	419	12	three	three	NUM
fnp-5883	419	13	different	different	ADJ
fnp-5883	419	14	freezing	freezing	NOUN
fnp-5883	419	15	methods	method	NOUN
fnp-5883	419	16	in	in	ADP
fnp-5883	419	17	the	the	DET
fnp-5883	419	18	protein	protein	NOUN
fnp-5883	419	19	profiles	profile	NOUN
fnp-5883	419	20	detected	detect	VERB
fnp-5883	419	21	by	by	ADP
fnp-5883	419	22	mass	mass	ADJ
fnp-5883	419	23	spectrometry	spectrometry	NOUN
fnp-5883	419	24	(	(	PUNCT
fnp-5883	419	25	meyronet	meyronet	NOUN
fnp-5883	419	26	et	et	PROPN
fnp-5883	419	27	al	al	PROPN
fnp-5883	419	28	.	.	PROPN
fnp-5883	419	29	,	,	PUNCT
fnp-5883	419	30	2015	2015	NUM
fnp-5883	419	31	)	)	PUNCT
fnp-5883	419	32	,	,	PUNCT
fnp-5883	419	33	even	even	ADV
fnp-5883	419	34	though	though	SCONJ
fnp-5883	419	35	there	there	PRON
fnp-5883	419	36	were	be	VERB
fnp-5883	419	37	differences	difference	NOUN
fnp-5883	419	38	in	in	ADP
fnp-5883	419	39	the	the	DET
fnp-5883	419	40	degrees	degree	NOUN
fnp-5883	419	41	of	of	ADP
fnp-5883	419	42	morphological	morphological	ADJ
fnp-5883	419	43	preservation	preservation	NOUN
fnp-5883	419	44	.	.	PUNCT
fnp-5883	420	1	it	it	PRON
fnp-5883	420	2	seems	seem	VERB
fnp-5883	420	3	that	that	SCONJ
fnp-5883	420	4	differences	difference	NOUN
fnp-5883	420	5	in	in	ADP
fnp-5883	420	6	cryopreservation	cryopreservation	NOUN
fnp-5883	420	7	protocols	protocol	NOUN
fnp-5883	420	8	have	have	VERB
fnp-5883	420	9	smaller	small	ADJ
fnp-5883	420	10	effects	effect	NOUN
fnp-5883	420	11	on	on	ADP
fnp-5883	420	12	many	many	ADJ
fnp-5883	420	13	biomolecular	biomolecular	ADJ
fnp-5883	420	14	features	feature	NOUN
fnp-5883	420	15	than	than	ADP
fnp-5883	420	16	on	on	ADP
fnp-5883	420	17	histology	histology	NOUN
fnp-5883	420	18	.	.	PUNCT
fnp-5883	421	1	for	for	ADP
fnp-5883	421	2	nucleic	nucleic	ADJ
fnp-5883	421	3	acids	acid	NOUN
fnp-5883	421	4	in	in	ADP
fnp-5883	421	5	particular	particular	ADJ
fnp-5883	421	6	,	,	PUNCT
fnp-5883	421	7	studies	study	NOUN
fnp-5883	421	8	have	have	AUX
fnp-5883	421	9	found	find	VERB
fnp-5883	421	10	that	that	SCONJ
fnp-5883	421	11	proxies	proxy	NOUN
fnp-5883	421	12	of	of	ADP
fnp-5883	421	13	a	a	DET
fnp-5883	421	14	prolonged	prolonged	ADJ
fnp-5883	421	15	and	and	CCONJ
fnp-5883	421	16	severe	severe	ADJ
fnp-5883	421	17	agonal	agonal	ADJ
fnp-5883	421	18	state	state	NOUN
fnp-5883	421	19	,	,	PUNCT
fnp-5883	421	20	such	such	ADJ
fnp-5883	421	21	as	as	ADP
fnp-5883	421	22	hospitalization	hospitalization	NOUN
fnp-5883	421	23	prior	prior	ADV
fnp-5883	421	24	to	to	ADP
fnp-5883	421	25	death	death	NOUN
fnp-5883	421	26	or	or	CCONJ
fnp-5883	421	27	decreased	decrease	VERB
fnp-5883	421	28	brain	brain	NOUN
fnp-5883	421	29	ph	ph	PROPN
fnp-5883	421	30	,	,	PUNCT
fnp-5883	421	31	are	be	AUX
fnp-5883	421	32	the	the	DET
fnp-5883	421	33	strongest	strong	ADJ
fnp-5883	421	34	correlate	correlate	NOUN
fnp-5883	421	35	of	of	ADP
fnp-5883	421	36	worsened	worsen	VERB
fnp-5883	421	37	rna	rna	NOUN
fnp-5883	421	38	quality	quality	NOUN
fnp-5883	421	39	(	(	PUNCT
fnp-5883	421	40	li	li	PROPN
fnp-5883	421	41	et	et	PROPN
fnp-5883	421	42	al	al	PROPN
fnp-5883	421	43	.	.	PROPN
fnp-5883	421	44	,	,	PUNCT
fnp-5883	421	45	2004	2004	NUM
fnp-5883	421	46	,	,	PUNCT
fnp-5883	421	47	2007	2007	NUM
fnp-5883	421	48	;	;	PUNCT
fnp-5883	421	49	durrenberger	durrenberger	NOUN
fnp-5883	421	50	et	et	PROPN
fnp-5883	421	51	al	al	PROPN
fnp-5883	421	52	.	.	PROPN
fnp-5883	421	53	,	,	PUNCT
fnp-5883	421	54	2010	2010	NUM
fnp-5883	421	55	;	;	PUNCT
fnp-5883	421	56	hagenauer	hagenauer	NOUN
fnp-5883	421	57	et	et	PROPN
fnp-5883	421	58	al	al	PROPN
fnp-5883	421	59	.	.	PROPN
fnp-5883	421	60	,	,	PUNCT
fnp-5883	421	61	2018	2018	NUM
fnp-5883	421	62	)	)	PUNCT
fnp-5883	421	63	.	.	PUNCT
fnp-5883	422	1	comparison	comparison	NOUN
fnp-5883	422	2	to	to	ADP
fnp-5883	422	3	other	other	ADJ
fnp-5883	422	4	tissues	tissue	NOUN
fnp-5883	422	5	several	several	ADJ
fnp-5883	422	6	studies	study	NOUN
fnp-5883	422	7	have	have	AUX
fnp-5883	422	8	noted	note	VERB
fnp-5883	422	9	that	that	SCONJ
fnp-5883	422	10	the	the	DET
fnp-5883	422	11	brain	brain	NOUN
fnp-5883	422	12	appears	appear	VERB
fnp-5883	422	13	to	to	PART
fnp-5883	422	14	be	be	AUX
fnp-5883	422	15	more	more	ADV
fnp-5883	422	16	vulnerable	vulnerable	ADJ
fnp-5883	422	17	to	to	ADP
fnp-5883	422	18	freezing	freeze	VERB
fnp-5883	422	19	damage	damage	NOUN
fnp-5883	422	20	than	than	ADP
fnp-5883	422	21	other	other	ADJ
fnp-5883	422	22	organs	organ	NOUN
fnp-5883	422	23	and	and	CCONJ
fnp-5883	422	24	tissues	tissue	NOUN
fnp-5883	422	25	.	.	PUNCT
fnp-5883	423	1	one	one	NUM
fnp-5883	423	2	source	source	NOUN
fnp-5883	423	3	found	find	VERB
fnp-5883	423	4	that	that	SCONJ
fnp-5883	423	5	while	while	SCONJ
fnp-5883	423	6	kidney	kidney	NOUN
fnp-5883	423	7	tissue	tissue	NOUN
fnp-5883	423	8	did	do	AUX
fnp-5883	423	9	not	not	PART
fnp-5883	423	10	show	show	VERB
fnp-5883	423	11	substantial	substantial	ADJ
fnp-5883	423	12	changes	change	NOUN
fnp-5883	423	13	after	after	ADP
fnp-5883	423	14	freezing	freeze	VERB
fnp-5883	423	15	,	,	PUNCT
fnp-5883	423	16	brain	brain	NOUN
fnp-5883	423	17	tissue	tissue	NOUN
fnp-5883	423	18	softened	soften	VERB
fnp-5883	423	19	significantly	significantly	ADV
fnp-5883	423	20	,	,	PUNCT
fnp-5883	423	21	making	make	VERB
fnp-5883	423	22	it	it	PRON
fnp-5883	423	23	difficult	difficult	ADJ
fnp-5883	423	24	to	to	PART
fnp-5883	423	25	process	process	VERB
fnp-5883	423	26	the	the	DET
fnp-5883	423	27	brain	brain	NOUN
fnp-5883	423	28	tissue	tissue	NOUN
fnp-5883	423	29	for	for	ADP
fnp-5883	423	30	evaluation	evaluation	NOUN
fnp-5883	423	31	(	(	PUNCT
fnp-5883	423	32	schäfer	schäfer	NOUN
fnp-5883	423	33	and	and	CCONJ
fnp-5883	423	34	kaufmann	kaufmann	PROPN
fnp-5883	423	35	,	,	PUNCT
fnp-5883	423	36	1999	1999	NUM
fnp-5883	423	37	)	)	PUNCT
fnp-5883	423	38	.	.	PUNCT
fnp-5883	424	1	these	these	DET
fnp-5883	424	2	authors	author	NOUN
fnp-5883	424	3	hypothesized	hypothesize	VERB
fnp-5883	424	4	that	that	SCONJ
fnp-5883	424	5	tissue	tissue	NOUN
fnp-5883	424	6	softening	soften	VERB
fnp-5883	424	7	after	after	SCONJ
fnp-5883	424	8	freezing	freeze	VERB
fnp-5883	424	9	occurs	occur	VERB
fnp-5883	424	10	in	in	ADP
fnp-5883	424	11	tissues	tissue	NOUN
fnp-5883	424	12	with	with	ADP
fnp-5883	424	13	high	high	ADJ
fnp-5883	424	14	extracellular	extracellular	ADJ
fnp-5883	424	15	water	water	NOUN
fnp-5883	424	16	content	content	NOUN
fnp-5883	424	17	and	and	CCONJ
fnp-5883	424	18	with	with	ADP
fnp-5883	424	19	relatively	relatively	ADV
fnp-5883	424	20	weak	weak	ADJ
fnp-5883	424	21	physical	physical	ADJ
fnp-5883	424	22	connections	connection	NOUN
fnp-5883	424	23	holding	hold	VERB
fnp-5883	424	24	the	the	DET
fnp-5883	424	25	tissue	tissue	NOUN
fnp-5883	424	26	together	together	ADV
fnp-5883	424	27	.	.	PUNCT
fnp-5883	425	1	they	they	PRON
fnp-5883	425	2	also	also	ADV
fnp-5883	425	3	noted	note	VERB
fnp-5883	425	4	that	that	SCONJ
fnp-5883	425	5	during	during	ADP
fnp-5883	425	6	slow	slow	ADJ
fnp-5883	425	7	freezing	freezing	NOUN
fnp-5883	425	8	,	,	PUNCT
fnp-5883	425	9	extracellular	extracellular	ADJ
fnp-5883	425	10	ice	ice	NOUN
fnp-5883	425	11	formation	formation	NOUN
fnp-5883	425	12	can	can	AUX
fnp-5883	425	13	cause	cause	VERB
fnp-5883	425	14	cells	cell	NOUN
fnp-5883	425	15	to	to	PART
fnp-5883	425	16	shrink	shrink	VERB
fnp-5883	425	17	significantly	significantly	ADV
fnp-5883	425	18	,	,	PUNCT
fnp-5883	425	19	potentially	potentially	ADV
fnp-5883	425	20	tearing	tear	VERB
fnp-5883	425	21	apart	apart	ADP
fnp-5883	425	22	cell	cell	NOUN
fnp-5883	425	23	-	-	PUNCT
fnp-5883	425	24	cell	cell	NOUN
fnp-5883	425	25	connections	connection	NOUN
fnp-5883	425	26	,	,	PUNCT
fnp-5883	425	27	which	which	PRON
fnp-5883	425	28	will	will	AUX
fnp-5883	425	29	not	not	PART
fnp-5883	425	30	redevelop	redevelop	VERB
fnp-5883	425	31	upon	upon	SCONJ
fnp-5883	425	32	thawing	thaw	VERB
fnp-5883	425	33	.	.	PUNCT
fnp-5883	426	1	another	another	DET
fnp-5883	426	2	source	source	NOUN
fnp-5883	426	3	observed	observe	VERB
fnp-5883	426	4	that	that	SCONJ
fnp-5883	426	5	freezing	freeze	VERB
fnp-5883	426	6	damage	damage	NOUN
fnp-5883	426	7	in	in	ADP
fnp-5883	426	8	the	the	DET
fnp-5883	426	9	brain	brain	NOUN
fnp-5883	426	10	seemed	seem	VERB
fnp-5883	426	11	to	to	PART
fnp-5883	426	12	be	be	AUX
fnp-5883	426	13	worse	bad	ADJ
fnp-5883	426	14	compared	compare	VERB
fnp-5883	426	15	to	to	ADP
fnp-5883	426	16	other	other	ADJ
fnp-5883	426	17	tissues	tissue	NOUN
fnp-5883	426	18	(	(	PUNCT
fnp-5883	426	19	lee	lee	PROPN
fnp-5883	426	20	et	et	PROPN
fnp-5883	426	21	al	al	PROPN
fnp-5883	426	22	.	.	PROPN
fnp-5883	426	23	,	,	PUNCT
fnp-5883	426	24	1997	1997	NUM
fnp-5883	426	25	)	)	PUNCT
fnp-5883	426	26	.	.	PUNCT
fnp-5883	427	1	the	the	DET
fnp-5883	427	2	authors	author	NOUN
fnp-5883	427	3	noted	note	VERB
fnp-5883	427	4	that	that	SCONJ
fnp-5883	427	5	the	the	DET
fnp-5883	427	6	brain	brain	NOUN
fnp-5883	427	7	appears	appear	VERB
fnp-5883	427	8	to	to	PART
fnp-5883	427	9	be	be	AUX
fnp-5883	427	10	the	the	DET
fnp-5883	427	11	“	"	PUNCT
fnp-5883	427	12	weak	weak	ADJ
fnp-5883	427	13	link	link	NOUN
fnp-5883	427	14	”	"	PUNCT
fnp-5883	427	15	with	with	ADP
fnp-5883	427	16	the	the	DET
fnp-5883	427	17	most	most	ADJ
fnp-5883	427	18	alterations	alteration	NOUN
fnp-5883	427	19	following	follow	VERB
fnp-5883	427	20	freezing	freeze	VERB
fnp-5883	427	21	in	in	ADP
fnp-5883	427	22	the	the	DET
fnp-5883	427	23	fly	fly	NOUN
fnp-5883	427	24	larvae	larvae	NOUN
fnp-5883	427	25	they	they	PRON
fnp-5883	427	26	were	be	AUX
fnp-5883	427	27	studying	study	VERB
fnp-5883	427	28	.	.	PUNCT
fnp-5883	428	1	finally	finally	ADV
fnp-5883	428	2	,	,	PUNCT
fnp-5883	428	3	one	one	NUM
fnp-5883	428	4	cryofixation	cryofixation	NOUN
fnp-5883	428	5	study	study	NOUN
fnp-5883	428	6	noted	note	VERB
fnp-5883	428	7	that	that	SCONJ
fnp-5883	428	8	nervous	nervous	ADJ
fnp-5883	428	9	system	system	NOUN
fnp-5883	428	10	tissue	tissue	NOUN
fnp-5883	428	11	is	be	AUX
fnp-5883	428	12	more	more	ADV
fnp-5883	428	13	difficult	difficult	ADJ
fnp-5883	428	14	to	to	PART
fnp-5883	428	15	vitrify	vitrify	VERB
fnp-5883	428	16	than	than	ADP
fnp-5883	428	17	any	any	DET
fnp-5883	428	18	other	other	ADJ
fnp-5883	428	19	tissue	tissue	NOUN
fnp-5883	428	20	they	they	PRON
fnp-5883	428	21	had	have	AUX
fnp-5883	428	22	studied	study	VERB
fnp-5883	428	23	(	(	PUNCT
fnp-5883	428	24	zuber	zuber	PROPN
fnp-5883	428	25	et	et	PROPN
fnp-5883	428	26	al	al	PROPN
fnp-5883	428	27	.	.	PROPN
fnp-5883	428	28	,	,	PUNCT
fnp-5883	428	29	2005	2005	NUM
fnp-5883	428	30	)	)	PUNCT
fnp-5883	428	31	.	.	PUNCT
fnp-5883	429	1	the	the	DET
fnp-5883	429	2	authors	author	NOUN
fnp-5883	429	3	reported	report	VERB
fnp-5883	429	4	that	that	SCONJ
fnp-5883	429	5	while	while	SCONJ
fnp-5883	429	6	tissues	tissue	NOUN
fnp-5883	429	7	like	like	ADP
fnp-5883	429	8	cartilage	cartilage	NOUN
fnp-5883	429	9	or	or	CCONJ
fnp-5883	429	10	skin	skin	NOUN
fnp-5883	429	11	could	could	AUX
fnp-5883	429	12	be	be	AUX
fnp-5883	429	13	vitrified	vitrify	VERB
fnp-5883	429	14	without	without	ADP
fnp-5883	429	15	cryoprotectants	cryoprotectant	NOUN
fnp-5883	429	16	,	,	PUNCT
fnp-5883	429	17	this	this	PRON
fnp-5883	429	18	was	be	AUX
fnp-5883	429	19	not	not	PART
fnp-5883	429	20	possible	possible	ADJ
fnp-5883	429	21	for	for	ADP
fnp-5883	429	22	nervous	nervous	ADJ
fnp-5883	429	23	tissue	tissue	NOUN
fnp-5883	429	24	,	,	PUNCT
fnp-5883	429	25	which	which	PRON
fnp-5883	429	26	required	require	VERB
fnp-5883	429	27	the	the	DET
fnp-5883	429	28	use	use	NOUN
fnp-5883	429	29	of	of	ADP
fnp-5883	429	30	cryoprotectants	cryoprotectant	NOUN
fnp-5883	429	31	for	for	ADP
fnp-5883	429	32	successful	successful	ADJ
fnp-5883	429	33	vitrification	vitrification	NOUN
fnp-5883	429	34	via	via	ADP
fnp-5883	429	35	cryofixation	cryofixation	NOUN
fnp-5883	429	36	.	.	PUNCT
fnp-5883	430	1	the	the	DET
fnp-5883	430	2	authors	author	NOUN
fnp-5883	430	3	attributed	attribute	VERB
fnp-5883	430	4	this	this	DET
fnp-5883	430	5	difficulty	difficulty	NOUN
fnp-5883	430	6	to	to	ADP
fnp-5883	430	7	the	the	DET
fnp-5883	430	8	brain	brain	NOUN
fnp-5883	430	9	's	's	PART
fnp-5883	430	10	high	high	ADJ
fnp-5883	430	11	content	content	NOUN
fnp-5883	430	12	of	of	ADP
fnp-5883	430	13	water	water	NOUN
fnp-5883	430	14	and	and	CCONJ
fnp-5883	430	15	lower	low	ADJ
fnp-5883	430	16	intrinsic	intrinsic	ADJ
fnp-5883	430	17	effect	effect	NOUN
fnp-5883	430	18	of	of	ADP
fnp-5883	430	19	cryoprotectants	cryoprotectant	NOUN
fnp-5883	430	20	.	.	PUNCT
fnp-5883	431	1	collectively	collectively	ADV
fnp-5883	431	2	,	,	PUNCT
fnp-5883	431	3	these	these	DET
fnp-5883	431	4	findings	finding	NOUN
fnp-5883	431	5	suggest	suggest	VERB
fnp-5883	431	6	that	that	SCONJ
fnp-5883	431	7	the	the	DET
fnp-5883	431	8	unique	unique	ADJ
fnp-5883	431	9	composition	composition	NOUN
fnp-5883	431	10	and	and	CCONJ
fnp-5883	431	11	structure	structure	NOUN
fnp-5883	431	12	of	of	ADP
fnp-5883	431	13	brain	brain	NOUN
fnp-5883	431	14	tissue	tissue	NOUN
fnp-5883	431	15	makes	make	VERB
fnp-5883	431	16	it	it	PRON
fnp-5883	431	17	particularly	particularly	ADV
fnp-5883	431	18	challenging	challenging	ADJ
fnp-5883	431	19	to	to	PART
fnp-5883	431	20	cryopreserve	cryopreserve	VERB
fnp-5883	431	21	brain	brain	NOUN
fnp-5883	431	22	tissue	tissue	NOUN
fnp-5883	431	23	effectively	effectively	ADV
fnp-5883	431	24	as	as	SCONJ
fnp-5883	431	25	compared	compare	VERB
fnp-5883	431	26	to	to	ADP
fnp-5883	431	27	many	many	ADJ
fnp-5883	431	28	other	other	ADJ
fnp-5883	431	29	tissue	tissue	NOUN
fnp-5883	431	30	types	type	NOUN
fnp-5883	431	31	.	.	PUNCT
fnp-5883	432	1	comparison	comparison	NOUN
fnp-5883	432	2	to	to	ADP
fnp-5883	432	3	previous	previous	ADJ
fnp-5883	432	4	reviews	review	NOUN
fnp-5883	432	5	to	to	ADP
fnp-5883	432	6	the	the	DET
fnp-5883	432	7	best	good	ADJ
fnp-5883	432	8	of	of	ADP
fnp-5883	432	9	our	our	PRON
fnp-5883	432	10	knowledge	knowledge	NOUN
fnp-5883	432	11	,	,	PUNCT
fnp-5883	432	12	there	there	PRON
fnp-5883	432	13	has	have	AUX
fnp-5883	432	14	not	not	PART
fnp-5883	432	15	been	be	AUX
fnp-5883	432	16	a	a	DET
fnp-5883	432	17	previous	previous	ADJ
fnp-5883	432	18	comprehensive	comprehensive	ADJ
fnp-5883	432	19	review	review	NOUN
fnp-5883	432	20	primarily	primarily	ADV
fnp-5883	432	21	on	on	ADP
fnp-5883	432	22	the	the	DET
fnp-5883	432	23	cryopreservation	cryopreservation	NOUN
fnp-5883	432	24	of	of	ADP
fnp-5883	432	25	brain	brain	NOUN
fnp-5883	432	26	tissue	tissue	NOUN
fnp-5883	432	27	.	.	PUNCT
fnp-5883	433	1	there	there	PRON
fnp-5883	433	2	have	have	AUX
fnp-5883	433	3	been	be	AUX
fnp-5883	433	4	many	many	ADJ
fnp-5883	433	5	previous	previous	ADJ
fnp-5883	433	6	reviews	review	NOUN
fnp-5883	433	7	on	on	ADP
fnp-5883	433	8	cryobiology	cryobiology	NOUN
fnp-5883	433	9	in	in	ADP
fnp-5883	433	10	general	general	ADJ
fnp-5883	433	11	,	,	PUNCT
fnp-5883	433	12	several	several	ADJ
fnp-5883	433	13	of	of	ADP
fnp-5883	433	14	which	which	PRON
fnp-5883	433	15	can	can	AUX
fnp-5883	433	16	be	be	AUX
fnp-5883	433	17	highlighted	highlight	VERB
fnp-5883	433	18	(	(	PUNCT
fnp-5883	433	19	bojic	bojic	NOUN
fnp-5883	433	20	et	et	PROPN
fnp-5883	433	21	al	al	PROPN
fnp-5883	433	22	.	.	PROPN
fnp-5883	433	23	,	,	PUNCT
fnp-5883	433	24	2021	2021	NUM
fnp-5883	433	25	;	;	PUNCT
fnp-5883	433	26	fahy	fahy	PROPN
fnp-5883	433	27	and	and	CCONJ
fnp-5883	433	28	wowk	wowk	NOUN
fnp-5883	433	29	,	,	PUNCT
fnp-5883	433	30	2021	2021	NUM
fnp-5883	433	31	;	;	PUNCT
fnp-5883	433	32	murray	murray	PROPN
fnp-5883	433	33	and	and	CCONJ
fnp-5883	433	34	gibson	gibson	PROPN
fnp-5883	433	35	,	,	PUNCT
fnp-5883	433	36	2022	2022	NUM
fnp-5883	433	37	)	)	PUNCT
fnp-5883	433	38	.	.	PUNCT
fnp-5883	434	1	in	in	ADP
fnp-5883	434	2	recent	recent	ADJ
fnp-5883	434	3	years	year	NOUN
fnp-5883	434	4	there	there	PRON
fnp-5883	434	5	has	have	AUX
fnp-5883	434	6	also	also	ADV
fnp-5883	434	7	been	be	AUX
fnp-5883	434	8	an	an	DET
fnp-5883	434	9	increased	increase	VERB
fnp-5883	434	10	focus	focus	NOUN
fnp-5883	434	11	on	on	ADP
fnp-5883	434	12	cryopreserving	cryopreserve	VERB
fnp-5883	434	13	complex	complex	ADJ
fnp-5883	434	14	tissues	tissue	NOUN
fnp-5883	434	15	or	or	CCONJ
fnp-5883	434	16	organs	organ	NOUN
fnp-5883	434	17	(	(	PUNCT
fnp-5883	434	18	giwa	giwa	NOUN
fnp-5883	434	19	et	et	PROPN
fnp-5883	434	20	al	al	PROPN
fnp-5883	434	21	.	.	PROPN
fnp-5883	434	22	,	,	PUNCT
fnp-5883	434	23	2017	2017	NUM
fnp-5883	434	24	)	)	PUNCT
fnp-5883	434	25	.	.	PUNCT
fnp-5883	435	1	however	however	ADV
fnp-5883	435	2	,	,	PUNCT
fnp-5883	435	3	these	these	DET
fnp-5883	435	4	reviews	review	NOUN
fnp-5883	435	5	have	have	AUX
fnp-5883	435	6	not	not	PART
fnp-5883	435	7	focused	focus	VERB
fnp-5883	435	8	on	on	ADP
fnp-5883	435	9	cryopreservation	cryopreservation	NOUN
fnp-5883	435	10	of	of	ADP
fnp-5883	435	11	brain	brain	NOUN
fnp-5883	435	12	tissue	tissue	NOUN
fnp-5883	435	13	,	,	PUNCT
fnp-5883	435	14	which	which	PRON
fnp-5883	435	15	is	be	AUX
fnp-5883	435	16	not	not	PART
fnp-5883	435	17	currently	currently	ADV
fnp-5883	435	18	considered	consider	VERB
fnp-5883	435	19	to	to	PART
fnp-5883	435	20	be	be	AUX
fnp-5883	435	21	as	as	ADV
fnp-5883	435	22	important	important	ADJ
fnp-5883	435	23	as	as	ADP
fnp-5883	435	24	other	other	ADJ
fnp-5883	435	25	tissue	tissue	NOUN
fnp-5883	435	26	types	type	NOUN
fnp-5883	435	27	for	for	ADP
fnp-5883	435	28	clinical	clinical	ADJ
fnp-5883	435	29	applications	application	NOUN
fnp-5883	435	30	such	such	ADJ
fnp-5883	435	31	as	as	ADP
fnp-5883	435	32	transplantation	transplantation	NOUN
fnp-5883	435	33	.	.	PUNCT
fnp-5883	436	1	the	the	DET
fnp-5883	436	2	reviews	review	NOUN
fnp-5883	436	3	on	on	ADP
fnp-5883	436	4	brain	brain	NOUN
fnp-5883	436	5	tissue	tissue	NOUN
fnp-5883	436	6	cryopreservation	cryopreservation	NOUN
fnp-5883	436	7	have	have	AUX
fnp-5883	436	8	tended	tend	VERB
fnp-5883	436	9	to	to	PART
fnp-5883	436	10	focus	focus	VERB
fnp-5883	436	11	on	on	ADP
fnp-5883	436	12	aspects	aspect	NOUN
fnp-5883	436	13	other	other	ADJ
fnp-5883	436	14	than	than	ADP
fnp-5883	436	15	histology	histology	NOUN
fnp-5883	436	16	,	,	PUNCT
fnp-5883	436	17	such	such	ADJ
fnp-5883	436	18	as	as	ADP
fnp-5883	436	19	biobanking	biobanking	NOUN
fnp-5883	436	20	in	in	ADP
fnp-5883	436	21	general	general	ADJ
fnp-5883	436	22	(	(	PUNCT
fnp-5883	436	23	shabihkhani	shabihkhani	PROPN
fnp-5883	436	24	et	et	PROPN
fnp-5883	436	25	al	al	PROPN
fnp-5883	436	26	.	.	PROPN
fnp-5883	436	27	,	,	PUNCT
fnp-5883	436	28	2014	2014	NUM
fnp-5883	436	29	)	)	PUNCT
fnp-5883	436	30	or	or	CCONJ
fnp-5883	436	31	cellular	cellular	ADJ
fnp-5883	436	32	survival	survival	NOUN
fnp-5883	436	33	(	(	PUNCT
fnp-5883	436	34	paynter	paynter	PROPN
fnp-5883	436	35	,	,	PUNCT
fnp-5883	436	36	2008	2008	NUM
fnp-5883	436	37	)	)	PUNCT
fnp-5883	436	38	.	.	PUNCT
fnp-5883	437	1	notably	notably	ADV
fnp-5883	437	2	,	,	PUNCT
fnp-5883	437	3	one	one	NUM
fnp-5883	437	4	review	review	NOUN
fnp-5883	437	5	notes	note	VERB
fnp-5883	437	6	that	that	SCONJ
fnp-5883	437	7	most	most	ADJ
fnp-5883	437	8	techniques	technique	NOUN
fnp-5883	437	9	on	on	ADP
fnp-5883	437	10	nerve	nerve	NOUN
fnp-5883	437	11	cell	cell	NOUN
fnp-5883	437	12	cryopreservation	cryopreservation	NOUN
fnp-5883	437	13	have	have	AUX
fnp-5883	437	14	been	be	AUX
fnp-5883	437	15	derived	derive	VERB
fnp-5883	437	16	from	from	ADP
fnp-5883	437	17	those	those	PRON
fnp-5883	437	18	applied	apply	VERB
fnp-5883	437	19	to	to	ADP
fnp-5883	437	20	cells	cell	NOUN
fnp-5883	437	21	of	of	ADP
fnp-5883	437	22	other	other	ADJ
fnp-5883	437	23	organs	organ	NOUN
fnp-5883	437	24	,	,	PUNCT
fnp-5883	437	25	rather	rather	ADV
fnp-5883	437	26	than	than	ADP
fnp-5883	437	27	being	be	AUX
fnp-5883	437	28	optimized	optimize	VERB
fnp-5883	437	29	for	for	ADP
fnp-5883	437	30	nerve	nerve	NOUN
fnp-5883	437	31	cells	cell	NOUN
fnp-5883	437	32	in	in	ADP
fnp-5883	437	33	particular	particular	ADJ
fnp-5883	437	34	(	(	PUNCT
fnp-5883	437	35	paynter	paynter	PROPN
fnp-5883	437	36	,	,	PUNCT
fnp-5883	437	37	2008	2008	NUM
fnp-5883	437	38	)	)	PUNCT
fnp-5883	437	39	.	.	PUNCT
fnp-5883	438	1	one	one	NUM
fnp-5883	438	2	review	review	NOUN
fnp-5883	438	3	article	article	NOUN
fnp-5883	438	4	primarily	primarily	ADV
fnp-5883	438	5	focusing	focus	VERB
fnp-5883	438	6	on	on	ADP
fnp-5883	438	7	biochemical	biochemical	ADJ
fnp-5883	438	8	and	and	CCONJ
fnp-5883	438	9	biomolecular	biomolecular	ADJ
fnp-5883	438	10	studies	study	NOUN
fnp-5883	438	11	in	in	ADP
fnp-5883	438	12	banked	banked	ADJ
fnp-5883	438	13	brain	brain	NOUN
fnp-5883	438	14	tissue	tissue	NOUN
fnp-5883	438	15	also	also	ADV
fnp-5883	438	16	reports	report	VERB
fnp-5883	438	17	that	that	SCONJ
fnp-5883	438	18	frozen	frozen	ADJ
fnp-5883	438	19	brain	brain	NOUN
fnp-5883	438	20	tissue	tissue	NOUN
fnp-5883	438	21	can	can	AUX
fnp-5883	438	22	been	be	AUX
fnp-5883	438	23	used	use	VERB
fnp-5883	438	24	in	in	ADP
fnp-5883	438	25	detailed	detailed	ADJ
fnp-5883	438	26	morphological	morphological	ADJ
fnp-5883	438	27	studies	study	NOUN
fnp-5883	438	28	(	(	PUNCT
fnp-5883	438	29	hynd	hynd	VERB
fnp-5883	438	30	et	et	PROPN
fnp-5883	438	31	al	al	PROPN
fnp-5883	438	32	.	.	PROPN
fnp-5883	438	33	,	,	PUNCT
fnp-5883	438	34	2003	2003	NUM
fnp-5883	438	35	)	)	PUNCT
fnp-5883	438	36	.	.	PUNCT
fnp-5883	439	1	finally	finally	ADV
fnp-5883	439	2	,	,	PUNCT
fnp-5883	439	3	one	one	NUM
fnp-5883	439	4	review	review	NOUN
fnp-5883	439	5	on	on	ADP
fnp-5883	439	6	the	the	DET
fnp-5883	439	7	practice	practice	NOUN
fnp-5883	439	8	of	of	ADP
fnp-5883	439	9	cryonics	cryonic	NOUN
fnp-5883	439	10	discusses	discuss	VERB
fnp-5883	439	11	the	the	DET
fnp-5883	439	12	cryopreservation	cryopreservation	NOUN
fnp-5883	439	13	of	of	ADP
fnp-5883	439	14	the	the	DET
fnp-5883	439	15	brain	brain	NOUN
fnp-5883	439	16	(	(	PUNCT
fnp-5883	439	17	best	good	ADJ
fnp-5883	439	18	,	,	PUNCT
fnp-5883	439	19	2008	2008	NUM
fnp-5883	439	20	)	)	PUNCT
fnp-5883	439	21	.	.	PUNCT
fnp-5883	440	1	the	the	DET
fnp-5883	440	2	author	author	NOUN
fnp-5883	440	3	primarily	primarily	ADV
fnp-5883	440	4	discusses	discuss	VERB
fnp-5883	440	5	articles	article	NOUN
fnp-5883	440	6	describing	describe	VERB
fnp-5883	440	7	successful	successful	ADJ
fnp-5883	440	8	preservation	preservation	NOUN
fnp-5883	440	9	of	of	ADP
fnp-5883	440	10	brain	brain	NOUN
fnp-5883	440	11	tissue	tissue	NOUN
fnp-5883	440	12	when	when	SCONJ
fnp-5883	440	13	cryoprotectants	cryoprotectant	NOUN
fnp-5883	440	14	are	be	AUX
fnp-5883	440	15	used	use	VERB
fnp-5883	440	16	.	.	PUNCT
fnp-5883	441	1	compared	compare	VERB
fnp-5883	441	2	to	to	ADP
fnp-5883	441	3	our	our	PRON
fnp-5883	441	4	review	review	NOUN
fnp-5883	441	5	,	,	PUNCT
fnp-5883	441	6	the	the	DET
fnp-5883	441	7	aforementioned	aforementioned	ADJ
fnp-5883	441	8	review	review	NOUN
fnp-5883	441	9	does	do	AUX
fnp-5883	441	10	not	not	PART
fnp-5883	441	11	extensively	extensively	ADV
fnp-5883	441	12	discuss	discuss	VERB
fnp-5883	441	13	studies	study	NOUN
fnp-5883	441	14	on	on	ADP
fnp-5883	441	15	the	the	DET
fnp-5883	441	16	histological	histological	ADJ
fnp-5883	441	17	outcomes	outcome	NOUN
fnp-5883	441	18	of	of	ADP
fnp-5883	441	19	freezing	freeze	VERB
fnp-5883	441	20	without	without	ADP
fnp-5883	441	21	cryoprotectants	cryoprotectant	NOUN
fnp-5883	441	22	,	,	PUNCT
fnp-5883	441	23	which	which	PRON
fnp-5883	441	24	will	will	AUX
fnp-5883	441	25	occur	occur	VERB
fnp-5883	441	26	if	if	SCONJ
fnp-5883	441	27	the	the	DET
fnp-5883	441	28	cryoprotectant	cryoprotectant	NOUN
fnp-5883	441	29	solution	solution	NOUN
fnp-5883	441	30	does	do	AUX
fnp-5883	441	31	not	not	PART
fnp-5883	441	32	reach	reach	VERB
fnp-5883	441	33	all	all	DET
fnp-5883	441	34	areas	area	NOUN
fnp-5883	441	35	of	of	ADP
fnp-5883	441	36	the	the	DET
fnp-5883	441	37	brain	brain	NOUN
fnp-5883	441	38	.	.	PUNCT
fnp-5883	442	1	limitations	limitation	NOUN
fnp-5883	442	2	of	of	ADP
fnp-5883	442	3	this	this	DET
fnp-5883	442	4	review	review	NOUN
fnp-5883	442	5	this	this	DET
fnp-5883	442	6	review	review	NOUN
fnp-5883	442	7	has	have	VERB
fnp-5883	442	8	a	a	DET
fnp-5883	442	9	number	number	NOUN
fnp-5883	442	10	of	of	ADP
fnp-5883	442	11	limitations	limitation	NOUN
fnp-5883	442	12	that	that	PRON
fnp-5883	442	13	should	should	AUX
fnp-5883	442	14	be	be	AUX
fnp-5883	442	15	considered	consider	VERB
fnp-5883	442	16	.	.	PUNCT
fnp-5883	443	1	first	first	ADV
fnp-5883	443	2	,	,	PUNCT
fnp-5883	443	3	the	the	DET
fnp-5883	443	4	data	datum	NOUN
fnp-5883	443	5	reported	report	VERB
fnp-5883	443	6	in	in	ADP
fnp-5883	443	7	some	some	DET
fnp-5883	443	8	articles	article	NOUN
fnp-5883	443	9	were	be	AUX
fnp-5883	443	10	occasionally	occasionally	ADV
fnp-5883	443	11	ambiguous	ambiguous	ADJ
fnp-5883	443	12	,	,	PUNCT
fnp-5883	443	13	making	make	VERB
fnp-5883	443	14	it	it	PRON
fnp-5883	443	15	difficult	difficult	ADJ
fnp-5883	443	16	to	to	PART
fnp-5883	443	17	tell	tell	VERB
fnp-5883	443	18	whether	whether	SCONJ
fnp-5883	443	19	the	the	DET
fnp-5883	443	20	observations	observation	NOUN
fnp-5883	443	21	were	be	AUX
fnp-5883	443	22	based	base	VERB
fnp-5883	443	23	on	on	ADP
fnp-5883	443	24	first	first	ADJ
fnp-5883	443	25	-	-	PUNCT
fnp-5883	443	26	hand	hand	NOUN
fnp-5883	443	27	experience	experience	NOUN
fnp-5883	443	28	or	or	CCONJ
fnp-5883	443	29	on	on	ADP
fnp-5883	443	30	second	second	ADJ
fnp-5883	443	31	-	-	PUNCT
fnp-5883	443	32	hand	hand	NOUN
fnp-5883	443	33	reports	report	NOUN
fnp-5883	443	34	.	.	PUNCT
fnp-5883	444	1	while	while	SCONJ
fnp-5883	444	2	we	we	PRON
fnp-5883	444	3	attempted	attempt	VERB
fnp-5883	444	4	to	to	PART
fnp-5883	444	5	include	include	VERB
fnp-5883	444	6	only	only	ADV
fnp-5883	444	7	primary	primary	ADJ
fnp-5883	444	8	data	datum	NOUN
fnp-5883	444	9	,	,	PUNCT
fnp-5883	444	10	this	this	DET
fnp-5883	444	11	ambiguity	ambiguity	NOUN
fnp-5883	444	12	may	may	AUX
fnp-5883	444	13	have	have	AUX
fnp-5883	444	14	introduced	introduce	VERB
fnp-5883	444	15	some	some	DET
fnp-5883	444	16	second	second	ADJ
fnp-5883	444	17	-	-	PUNCT
fnp-5883	444	18	hand	hand	NOUN
fnp-5883	444	19	data	datum	NOUN
fnp-5883	444	20	into	into	ADP
fnp-5883	444	21	our	our	PRON
fnp-5883	444	22	findings	finding	NOUN
fnp-5883	444	23	.	.	PUNCT
fnp-5883	445	1	second	second	ADJ
fnp-5883	445	2	,	,	PUNCT
fnp-5883	445	3	our	our	PRON
fnp-5883	445	4	review	review	NOUN
fnp-5883	445	5	focused	focus	VERB
fnp-5883	445	6	primarily	primarily	ADV
fnp-5883	445	7	on	on	ADP
fnp-5883	445	8	brain	brain	NOUN
fnp-5883	445	9	tissue	tissue	NOUN
fnp-5883	445	10	cryopreservation	cryopreservation	NOUN
fnp-5883	445	11	,	,	PUNCT
fnp-5883	445	12	with	with	ADP
fnp-5883	445	13	limited	limited	ADJ
fnp-5883	445	14	consideration	consideration	NOUN
fnp-5883	445	15	of	of	ADP
fnp-5883	445	16	other	other	ADJ
fnp-5883	445	17	tissue	tissue	NOUN
fnp-5883	445	18	types	type	NOUN
fnp-5883	445	19	.	.	PUNCT
fnp-5883	446	1	given	give	VERB
fnp-5883	446	2	the	the	DET
fnp-5883	446	3	potential	potential	ADJ
fnp-5883	446	4	similarities	similarity	NOUN
fnp-5883	446	5	across	across	ADP
fnp-5883	446	6	tissues	tissue	NOUN
fnp-5883	446	7	,	,	PUNCT
fnp-5883	446	8	a	a	DET
fnp-5883	446	9	more	more	ADV
fnp-5883	446	10	comprehensive	comprehensive	ADJ
fnp-5883	446	11	review	review	NOUN
fnp-5883	446	12	incorporating	incorporate	VERB
fnp-5883	446	13	the	the	DET
fnp-5883	446	14	broader	broad	ADJ
fnp-5883	446	15	literature	literature	NOUN
fnp-5883	446	16	could	could	AUX
fnp-5883	446	17	provide	provide	VERB
fnp-5883	446	18	additional	additional	ADJ
fnp-5883	446	19	insights	insight	NOUN
fnp-5883	446	20	.	.	PUNCT
fnp-5883	447	1	third	third	ADJ
fnp-5883	447	2	,	,	PUNCT
fnp-5883	447	3	the	the	DET
fnp-5883	447	4	“	"	PUNCT
fnp-5883	447	5	grey	grey	ADJ
fnp-5883	447	6	”	"	PUNCT
fnp-5883	447	7	literature	literature	NOUN
fnp-5883	447	8	on	on	ADP
fnp-5883	447	9	this	this	DET
fnp-5883	447	10	technical	technical	ADJ
fnp-5883	447	11	topic	topic	NOUN
fnp-5883	447	12	,	,	PUNCT
fnp-5883	447	13	such	such	ADJ
fnp-5883	447	14	as	as	ADP
fnp-5883	447	15	information	information	NOUN
fnp-5883	447	16	shared	share	VERB
fnp-5883	447	17	on	on	ADP
fnp-5883	447	18	mailing	mailing	NOUN
fnp-5883	447	19	lists	list	NOUN
fnp-5883	447	20	and	and	CCONJ
fnp-5883	447	21	platforms	platform	NOUN
fnp-5883	447	22	like	like	ADP
fnp-5883	447	23	researchgate	researchgate	NOUN
fnp-5883	447	24	,	,	PUNCT
fnp-5883	447	25	was	be	AUX
fnp-5883	447	26	not	not	PART
fnp-5883	447	27	included	include	VERB
fnp-5883	447	28	in	in	ADP
fnp-5883	447	29	our	our	PRON
fnp-5883	447	30	review	review	NOUN
fnp-5883	447	31	.	.	PUNCT
fnp-5883	448	1	future	future	ADJ
fnp-5883	448	2	reviews	review	NOUN
fnp-5883	448	3	may	may	AUX
fnp-5883	448	4	benefit	benefit	VERB
fnp-5883	448	5	from	from	ADP
fnp-5883	448	6	carefully	carefully	ADV
fnp-5883	448	7	considering	consider	VERB
fnp-5883	448	8	these	these	DET
fnp-5883	448	9	unpublished	unpublished	ADJ
fnp-5883	448	10	data	datum	NOUN
fnp-5883	448	11	,	,	PUNCT
fnp-5883	448	12	as	as	SCONJ
fnp-5883	448	13	they	they	PRON
fnp-5883	448	14	may	may	AUX
fnp-5883	448	15	contain	contain	VERB
fnp-5883	448	16	valuable	valuable	ADJ
fnp-5883	448	17	insights	insight	NOUN
fnp-5883	448	18	from	from	ADP
fnp-5883	448	19	researchers	researcher	NOUN
fnp-5883	448	20	who	who	PRON
fnp-5883	448	21	have	have	AUX
fnp-5883	448	22	addressed	address	VERB
fnp-5883	448	23	the	the	DET
fnp-5883	448	24	topic	topic	NOUN
fnp-5883	448	25	in	in	ADP
fnp-5883	448	26	practice	practice	NOUN
fnp-5883	448	27	without	without	ADP
fnp-5883	448	28	formally	formally	ADV
fnp-5883	448	29	publishing	publish	VERB
fnp-5883	448	30	their	their	PRON
fnp-5883	448	31	findings	finding	NOUN
fnp-5883	448	32	.	.	PUNCT
fnp-5883	449	1	fourth	fourth	ADJ
fnp-5883	449	2	,	,	PUNCT
fnp-5883	449	3	the	the	DET
fnp-5883	449	4	distinctions	distinction	NOUN
fnp-5883	449	5	between	between	ADP
fnp-5883	449	6	the	the	DET
fnp-5883	449	7	different	different	ADJ
fnp-5883	449	8	categories	category	NOUN
fnp-5883	449	9	of	of	ADP
fnp-5883	449	10	cryopreservation	cryopreservation	NOUN
fnp-5883	449	11	techniques	technique	NOUN
fnp-5883	449	12	of	of	ADP
fnp-5883	449	13	cryofixation	cryofixation	NOUN
fnp-5883	449	14	,	,	PUNCT
fnp-5883	449	15	freezing	freezing	NOUN
fnp-5883	449	16	,	,	PUNCT
fnp-5883	449	17	and	and	CCONJ
fnp-5883	449	18	cryopreservation	cryopreservation	NOUN
fnp-5883	449	19	with	with	ADP
fnp-5883	449	20	cryoprotectants	cryoprotectant	NOUN
fnp-5883	449	21	can	can	AUX
fnp-5883	449	22	be	be	AUX
fnp-5883	449	23	somewhat	somewhat	ADV
fnp-5883	449	24	blurred	blur	VERB
fnp-5883	449	25	,	,	PUNCT
fnp-5883	449	26	as	as	SCONJ
fnp-5883	449	27	some	some	DET
fnp-5883	449	28	studies	study	NOUN
fnp-5883	449	29	may	may	AUX
fnp-5883	449	30	employ	employ	VERB
fnp-5883	449	31	methods	method	NOUN
fnp-5883	449	32	that	that	PRON
fnp-5883	449	33	overlap	overlap	VERB
fnp-5883	449	34	or	or	CCONJ
fnp-5883	449	35	share	share	NOUN
fnp-5883	449	36	similarities	similarity	NOUN
fnp-5883	449	37	across	across	ADP
fnp-5883	449	38	these	these	DET
fnp-5883	449	39	categories	category	NOUN
fnp-5883	449	40	.	.	PUNCT
fnp-5883	450	1	for	for	ADP
fnp-5883	450	2	example	example	NOUN
fnp-5883	450	3	,	,	PUNCT
fnp-5883	450	4	some	some	DET
fnp-5883	450	5	cryofixation	cryofixation	NOUN
fnp-5883	450	6	studies	study	NOUN
fnp-5883	450	7	use	use	VERB
fnp-5883	450	8	cryoprotectants	cryoprotectant	NOUN
fnp-5883	450	9	.	.	PUNCT
fnp-5883	451	1	this	this	DET
fnp-5883	451	2	potential	potential	ADJ
fnp-5883	451	3	ambiguity	ambiguity	NOUN
fnp-5883	451	4	in	in	ADP
fnp-5883	451	5	categorization	categorization	NOUN
fnp-5883	451	6	may	may	AUX
fnp-5883	451	7	have	have	AUX
fnp-5883	451	8	influenced	influence	VERB
fnp-5883	451	9	the	the	DET
fnp-5883	451	10	interpretation	interpretation	NOUN
fnp-5883	451	11	and	and	CCONJ
fnp-5883	451	12	synthesis	synthesis	NOUN
fnp-5883	451	13	of	of	ADP
fnp-5883	451	14	the	the	DET
fnp-5883	451	15	reviewed	review	VERB
fnp-5883	451	16	studies	study	NOUN
fnp-5883	451	17	.	.	PUNCT
fnp-5883	452	1	fifth	fifth	ADJ
fnp-5883	452	2	,	,	PUNCT
fnp-5883	452	3	our	our	PRON
fnp-5883	452	4	assessment	assessment	NOUN
fnp-5883	452	5	of	of	ADP
fnp-5883	452	6	preservation	preservation	NOUN
fnp-5883	452	7	quality	quality	NOUN
fnp-5883	452	8	relies	rely	VERB
fnp-5883	452	9	heavily	heavily	ADV
fnp-5883	452	10	on	on	ADP
fnp-5883	452	11	the	the	DET
fnp-5883	452	12	verbal	verbal	ADJ
fnp-5883	452	13	reports	report	NOUN
fnp-5883	452	14	and	and	CCONJ
fnp-5883	452	15	descriptions	description	NOUN
fnp-5883	452	16	provided	provide	VERB
fnp-5883	452	17	by	by	ADP
fnp-5883	452	18	the	the	DET
fnp-5883	452	19	authors	author	NOUN
fnp-5883	452	20	of	of	ADP
fnp-5883	452	21	the	the	DET
fnp-5883	452	22	reviewed	review	VERB
fnp-5883	452	23	studies	study	NOUN
fnp-5883	452	24	.	.	PUNCT
fnp-5883	453	1	the	the	DET
fnp-5883	453	2	lack	lack	NOUN
fnp-5883	453	3	of	of	ADP
fnp-5883	453	4	standardized	standardized	ADJ
fnp-5883	453	5	,	,	PUNCT
fnp-5883	453	6	quantitative	quantitative	ADJ
fnp-5883	453	7	metrics	metric	NOUN
fnp-5883	453	8	for	for	ADP
fnp-5883	453	9	evaluating	evaluate	VERB
fnp-5883	453	10	preservation	preservation	NOUN
fnp-5883	453	11	quality	quality	NOUN
fnp-5883	453	12	across	across	ADP
fnp-5883	453	13	studies	study	NOUN
fnp-5883	453	14	limits	limit	VERB
fnp-5883	453	15	the	the	DET
fnp-5883	453	16	objectivity	objectivity	NOUN
fnp-5883	453	17	and	and	CCONJ
fnp-5883	453	18	comparability	comparability	NOUN
fnp-5883	453	19	of	of	ADP
fnp-5883	453	20	these	these	DET
fnp-5883	453	21	assessments	assessment	NOUN
fnp-5883	453	22	.	.	PUNCT
fnp-5883	454	1	overall	overall	ADV
fnp-5883	454	2	,	,	PUNCT
fnp-5883	454	3	the	the	DET
fnp-5883	454	4	literature	literature	NOUN
fnp-5883	454	5	on	on	ADP
fnp-5883	454	6	morphological	morphological	ADJ
fnp-5883	454	7	outcomes	outcome	NOUN
fnp-5883	454	8	following	follow	VERB
fnp-5883	454	9	cryopreservation	cryopreservation	NOUN
fnp-5883	454	10	of	of	ADP
fnp-5883	454	11	unfixed	unfixed	ADJ
fnp-5883	454	12	brain	brain	NOUN
fnp-5883	454	13	tissue	tissue	NOUN
fnp-5883	454	14	is	be	AUX
fnp-5883	454	15	relatively	relatively	ADV
fnp-5883	454	16	limited	limited	ADJ
fnp-5883	454	17	compared	compare	VERB
fnp-5883	454	18	to	to	ADP
fnp-5883	454	19	studies	study	NOUN
fnp-5883	454	20	using	use	VERB
fnp-5883	454	21	fixed	fix	VERB
fnp-5883	454	22	tissue	tissue	NOUN
fnp-5883	454	23	,	,	PUNCT
fnp-5883	454	24	particularly	particularly	ADV
fnp-5883	454	25	outside	outside	ADV
fnp-5883	454	26	of	of	ADP
fnp-5883	454	27	the	the	DET
fnp-5883	454	28	cryofixation	cryofixation	NOUN
fnp-5883	454	29	domain	domain	NOUN
fnp-5883	454	30	.	.	PUNCT
fnp-5883	455	1	the	the	DET
fnp-5883	455	2	majority	majority	NOUN
fnp-5883	455	3	of	of	ADP
fnp-5883	455	4	the	the	DET
fnp-5883	455	5	reviewed	review	VERB
fnp-5883	455	6	studies	study	NOUN
fnp-5883	455	7	have	have	VERB
fnp-5883	455	8	small	small	ADJ
fnp-5883	455	9	sample	sample	NOUN
fnp-5883	455	10	sizes	size	NOUN
fnp-5883	455	11	and	and	CCONJ
fnp-5883	455	12	limited	limited	ADJ
fnp-5883	455	13	replication	replication	NOUN
fnp-5883	455	14	,	,	PUNCT
fnp-5883	455	15	which	which	PRON
fnp-5883	455	16	may	may	AUX
fnp-5883	455	17	affect	affect	VERB
fnp-5883	455	18	the	the	DET
fnp-5883	455	19	generalizability	generalizability	NOUN
fnp-5883	455	20	of	of	ADP
fnp-5883	455	21	their	their	PRON
fnp-5883	455	22	findings	finding	NOUN
fnp-5883	455	23	.	.	PUNCT
fnp-5883	456	1	as	as	ADP
fnp-5883	456	2	a	a	DET
fnp-5883	456	3	result	result	NOUN
fnp-5883	456	4	,	,	PUNCT
fnp-5883	456	5	the	the	DET
fnp-5883	456	6	conclusions	conclusion	NOUN
fnp-5883	456	7	drawn	draw	VERB
fnp-5883	456	8	in	in	ADP
fnp-5883	456	9	this	this	DET
fnp-5883	456	10	review	review	NOUN
fnp-5883	456	11	should	should	AUX
fnp-5883	456	12	be	be	AUX
fnp-5883	456	13	interpreted	interpret	VERB
fnp-5883	456	14	with	with	ADP
fnp-5883	456	15	caution	caution	NOUN
fnp-5883	456	16	,	,	PUNCT
fnp-5883	456	17	as	as	SCONJ
fnp-5883	456	18	they	they	PRON
fnp-5883	456	19	are	be	AUX
fnp-5883	456	20	based	base	VERB
fnp-5883	456	21	on	on	ADP
fnp-5883	456	22	a	a	DET
fnp-5883	456	23	relatively	relatively	ADV
fnp-5883	456	24	small	small	ADJ
fnp-5883	456	25	number	number	NOUN
fnp-5883	456	26	of	of	ADP
fnp-5883	456	27	studies	study	NOUN
fnp-5883	456	28	.	.	PUNCT
fnp-5883	457	1	further	further	ADJ
fnp-5883	457	2	research	research	NOUN
fnp-5883	457	3	with	with	ADP
fnp-5883	457	4	larger	large	ADJ
fnp-5883	457	5	sample	sample	NOUN
fnp-5883	457	6	sizes	size	NOUN
fnp-5883	457	7	and	and	CCONJ
fnp-5883	457	8	standardized	standardized	ADJ
fnp-5883	457	9	evaluation	evaluation	NOUN
fnp-5883	457	10	methods	method	NOUN
fnp-5883	457	11	is	be	AUX
fnp-5883	457	12	needed	need	VERB
fnp-5883	457	13	to	to	PART
fnp-5883	457	14	corroborate	corroborate	VERB
fnp-5883	457	15	and	and	CCONJ
fnp-5883	457	16	extend	extend	VERB
fnp-5883	457	17	the	the	DET
fnp-5883	457	18	findings	finding	NOUN
fnp-5883	457	19	of	of	ADP
fnp-5883	457	20	this	this	DET
fnp-5883	457	21	review	review	NOUN
fnp-5883	457	22	.	.	PUNCT
fnp-5883	458	1	summary	summary	NOUN
fnp-5883	458	2	of	of	ADP
fnp-5883	458	3	cryopreservation	cryopreservation	NOUN
fnp-5883	458	4	methods	method	NOUN
fnp-5883	458	5	discussed	discuss	VERB
fnp-5883	458	6	we	we	PRON
fnp-5883	458	7	can	can	AUX
fnp-5883	458	8	summarize	summarize	VERB
fnp-5883	458	9	several	several	ADJ
fnp-5883	458	10	of	of	ADP
fnp-5883	458	11	the	the	DET
fnp-5883	458	12	cryopreservation	cryopreservation	NOUN
fnp-5883	458	13	methods	method	NOUN
fnp-5883	458	14	discussed	discuss	VERB
fnp-5883	458	15	in	in	ADP
fnp-5883	458	16	this	this	DET
fnp-5883	458	17	article	article	NOUN
fnp-5883	458	18	(	(	PUNCT
fnp-5883	458	19	table	table	NOUN
fnp-5883	458	20	 	 	SPACE
fnp-5883	458	21	6	6	NUM
fnp-5883	458	22	)	)	PUNCT
fnp-5883	458	23	.	.	PUNCT
fnp-5883	459	1	for	for	ADP
fnp-5883	459	2	smaller	small	ADJ
fnp-5883	459	3	specimens	specimen	NOUN
fnp-5883	459	4	such	such	ADJ
fnp-5883	459	5	as	as	ADP
fnp-5883	459	6	tissue	tissue	NOUN
fnp-5883	459	7	biopsy	biopsy	NOUN
fnp-5883	459	8	samples	sample	NOUN
fnp-5883	459	9	,	,	PUNCT
fnp-5883	459	10	cryofixation	cryofixation	NOUN
fnp-5883	459	11	or	or	CCONJ
fnp-5883	459	12	cryoprotectant	cryoprotectant	NOUN
fnp-5883	459	13	-	-	PUNCT
fnp-5883	459	14	based	base	VERB
fnp-5883	459	15	methods	method	NOUN
fnp-5883	459	16	likely	likely	ADV
fnp-5883	459	17	offer	offer	VERB
fnp-5883	459	18	the	the	DET
fnp-5883	459	19	best	good	ADJ
fnp-5883	459	20	histologic	histologic	ADJ
fnp-5883	459	21	preservation	preservation	NOUN
fnp-5883	459	22	,	,	PUNCT
fnp-5883	459	23	especially	especially	ADV
fnp-5883	459	24	as	as	SCONJ
fnp-5883	459	25	viewed	view	VERB
fnp-5883	459	26	under	under	ADP
fnp-5883	459	27	the	the	DET
fnp-5883	459	28	electron	electron	NOUN
fnp-5883	459	29	microscope	microscope	NOUN
fnp-5883	459	30	.	.	PUNCT
fnp-5883	460	1	however	however	ADV
fnp-5883	460	2	,	,	PUNCT
fnp-5883	460	3	for	for	ADP
fnp-5883	460	4	the	the	DET
fnp-5883	460	5	larger	large	ADJ
fnp-5883	460	6	whole	whole	ADJ
fnp-5883	460	7	brain	brain	NOUN
fnp-5883	460	8	or	or	CCONJ
fnp-5883	460	9	hemisphere	hemisphere	ADJ
fnp-5883	460	10	slices	slice	NOUN
fnp-5883	460	11	that	that	PRON
fnp-5883	460	12	are	be	AUX
fnp-5883	460	13	typically	typically	ADV
fnp-5883	460	14	preserved	preserve	VERB
fnp-5883	460	15	in	in	ADP
fnp-5883	460	16	brain	brain	NOUN
fnp-5883	460	17	banking	banking	NOUN
fnp-5883	460	18	,	,	PUNCT
fnp-5883	460	19	freezing	freezing	ADJ
fnp-5883	460	20	methods	method	NOUN
fnp-5883	460	21	are	be	AUX
fnp-5883	460	22	the	the	DET
fnp-5883	460	23	only	only	ADJ
fnp-5883	460	24	published	publish	VERB
fnp-5883	460	25	methods	method	NOUN
fnp-5883	460	26	that	that	PRON
fnp-5883	460	27	have	have	AUX
fnp-5883	460	28	been	be	AUX
fnp-5883	460	29	reported	report	VERB
fnp-5883	460	30	to	to	PART
fnp-5883	460	31	be	be	AUX
fnp-5883	460	32	reliably	reliably	ADV
fnp-5883	460	33	effective	effective	ADJ
fnp-5883	460	34	.	.	PUNCT
fnp-5883	461	1	among	among	ADP
fnp-5883	461	2	the	the	DET
fnp-5883	461	3	cryopreservation	cryopreservation	NOUN
fnp-5883	461	4	methods	method	NOUN
fnp-5883	461	5	for	for	ADP
fnp-5883	461	6	brain	brain	NOUN
fnp-5883	461	7	slices	slice	NOUN
fnp-5883	461	8	,	,	PUNCT
fnp-5883	461	9	freezing	freeze	VERB
fnp-5883	461	10	between	between	ADP
fnp-5883	461	11	cooled	cool	VERB
fnp-5883	461	12	metal	metal	NOUN
fnp-5883	461	13	plates	plate	NOUN
fnp-5883	461	14	,	,	PUNCT
fnp-5883	461	15	embedding	embed	VERB
fnp-5883	461	16	in	in	ADP
fnp-5883	461	17	dry	dry	ADJ
fnp-5883	461	18	ice	ice	NOUN
fnp-5883	461	19	,	,	PUNCT
fnp-5883	461	20	or	or	CCONJ
fnp-5883	461	21	immersion	immersion	NOUN
fnp-5883	461	22	in	in	ADP
fnp-5883	461	23	cooled	cool	VERB
fnp-5883	461	24	isopentane	isopentane	NOUN
fnp-5883	461	25	appear	appear	VERB
fnp-5883	461	26	to	to	PART
fnp-5883	461	27	be	be	AUX
fnp-5883	461	28	the	the	DET
fnp-5883	461	29	most	most	ADV
fnp-5883	461	30	effective	effective	ADJ
fnp-5883	461	31	for	for	ADP
fnp-5883	461	32	preserving	preserve	VERB
fnp-5883	461	33	relatively	relatively	ADV
fnp-5883	461	34	large	large	ADJ
fnp-5883	461	35	samples	sample	NOUN
fnp-5883	461	36	while	while	SCONJ
fnp-5883	461	37	minimizing	minimize	VERB
fnp-5883	461	38	ice	ice	NOUN
fnp-5883	461	39	artifacts	artifact	NOUN
fnp-5883	461	40	as	as	SCONJ
fnp-5883	461	41	seen	see	VERB
fnp-5883	461	42	on	on	ADP
fnp-5883	461	43	light	light	ADJ
fnp-5883	461	44	microscopy	microscopy	NOUN
fnp-5883	461	45	(	(	PUNCT
fnp-5883	461	46	vonsattel	vonsattel	NOUN
fnp-5883	461	47	et	et	PROPN
fnp-5883	461	48	al	al	PROPN
fnp-5883	461	49	.	.	PROPN
fnp-5883	461	50	,	,	PUNCT
fnp-5883	461	51	2008	2008	NUM
fnp-5883	461	52	;	;	PUNCT
fnp-5883	461	53	meyronet	meyronet	NOUN
fnp-5883	461	54	et	et	PROPN
fnp-5883	461	55	al	al	PROPN
fnp-5883	461	56	.	.	PROPN
fnp-5883	461	57	,	,	PUNCT
fnp-5883	461	58	2015	2015	NUM
fnp-5883	461	59	)	)	PUNCT
fnp-5883	461	60	.	.	PUNCT
fnp-5883	462	1	notably	notably	ADV
fnp-5883	462	2	,	,	PUNCT
fnp-5883	462	3	many	many	ADJ
fnp-5883	462	4	investigators	investigator	NOUN
fnp-5883	462	5	do	do	AUX
fnp-5883	462	6	not	not	PART
fnp-5883	462	7	focus	focus	VERB
fnp-5883	462	8	on	on	ADP
fnp-5883	462	9	detailed	detailed	ADJ
fnp-5883	462	10	morphological	morphological	ADJ
fnp-5883	462	11	preservation	preservation	NOUN
fnp-5883	462	12	for	for	ADP
fnp-5883	462	13	their	their	PRON
fnp-5883	462	14	frozen	frozen	ADJ
fnp-5883	462	15	tissue	tissue	NOUN
fnp-5883	462	16	,	,	PUNCT
fnp-5883	462	17	focusing	focus	VERB
fnp-5883	462	18	instead	instead	ADV
fnp-5883	462	19	on	on	ADP
fnp-5883	462	20	preservation	preservation	NOUN
fnp-5883	462	21	of	of	ADP
fnp-5883	462	22	biochemical	biochemical	ADJ
fnp-5883	462	23	properties	property	NOUN
fnp-5883	462	24	,	,	PUNCT
fnp-5883	462	25	making	make	VERB
fnp-5883	462	26	the	the	DET
fnp-5883	462	27	histologic	histologic	ADJ
fnp-5883	462	28	considerations	consideration	NOUN
fnp-5883	462	29	less	less	ADV
fnp-5883	462	30	important	important	ADJ
fnp-5883	462	31	.	.	PUNCT
fnp-5883	463	1	method	method	NOUN
fnp-5883	463	2	benefits	benefit	NOUN
fnp-5883	463	3	drawbacks	drawback	NOUN
fnp-5883	463	4	cryofixation	cryofixation	NOUN
fnp-5883	463	5	•	•	ADV
fnp-5883	463	6	excellent	excellent	ADJ
fnp-5883	463	7	ultrastructural	ultrastructural	ADJ
fnp-5883	463	8	preservation	preservation	NOUN
fnp-5883	463	9	•	•	ADP
fnp-5883	463	10	minimal	minimal	ADJ
fnp-5883	463	11	ice	ice	NOUN
fnp-5883	463	12	artifacts	artifact	NOUN
fnp-5883	463	13	in	in	ADP
fnp-5883	463	14	vitrified	vitrified	ADJ
fnp-5883	463	15	regions	region	NOUN
fnp-5883	463	16	•	•	ADV
fnp-5883	463	17	limited	limit	VERB
fnp-5883	463	18	to	to	ADP
fnp-5883	463	19	very	very	ADV
fnp-5883	463	20	thin	thin	ADJ
fnp-5883	463	21	samples	sample	NOUN
fnp-5883	463	22	,	,	PUNCT
fnp-5883	463	23	typically	typically	ADV
fnp-5883	463	24	˜20–200	˜20–200	PROPN
fnp-5883	463	25	 	 	SPACE
fnp-5883	463	26	μm	μm	NUM
fnp-5883	463	27	or	or	CCONJ
fnp-5883	463	28	less	less	ADJ
fnp-5883	463	29	•	•	NOUN
fnp-5883	463	30	requires	require	VERB
fnp-5883	463	31	specialized	specialized	ADJ
fnp-5883	463	32	equipment	equipment	NOUN
fnp-5883	463	33	direct	direct	ADJ
fnp-5883	463	34	placement	placement	NOUN
fnp-5883	463	35	in	in	ADP
fnp-5883	463	36	freezer	freezer	NOUN
fnp-5883	463	37	•	•	ADP
fnp-5883	463	38	simple	simple	ADJ
fnp-5883	463	39	,	,	PUNCT
fnp-5883	463	40	accessible	accessible	ADJ
fnp-5883	463	41	,	,	PUNCT
fnp-5883	463	42	and	and	CCONJ
fnp-5883	463	43	low	low	ADJ
fnp-5883	463	44	-	-	PUNCT
fnp-5883	463	45	cost	cost	NOUN
fnp-5883	463	46	•	•	NOUN
fnp-5883	463	47	easily	easily	ADV
fnp-5883	463	48	integrates	integrate	NOUN
fnp-5883	463	49	with	with	ADP
fnp-5883	463	50	long	long	ADJ
fnp-5883	463	51	-	-	PUNCT
fnp-5883	463	52	term	term	NOUN
fnp-5883	463	53	storage	storage	NOUN
fnp-5883	463	54	•	•	NOUN
fnp-5883	463	55	slow	slow	ADJ
fnp-5883	463	56	cooling	cool	VERB
fnp-5883	463	57	rate	rate	NOUN
fnp-5883	463	58	due	due	ADP
fnp-5883	463	59	to	to	ADP
fnp-5883	463	60	poor	poor	ADJ
fnp-5883	463	61	thermal	thermal	ADJ
fnp-5883	463	62	conductivity	conductivity	NOUN
fnp-5883	463	63	leads	lead	VERB
fnp-5883	463	64	to	to	ADP
fnp-5883	463	65	severe	severe	ADJ
fnp-5883	463	66	ice	ice	NOUN
fnp-5883	463	67	artifacts	artifact	NOUN
fnp-5883	463	68	and	and	CCONJ
fnp-5883	463	69	poor	poor	ADJ
fnp-5883	463	70	histologic	histologic	ADJ
fnp-5883	463	71	preservation	preservation	NOUN
fnp-5883	463	72	freezing	freeze	VERB
fnp-5883	463	73	in	in	ADP
fnp-5883	463	74	dry	dry	ADJ
fnp-5883	463	75	ice	ice	NOUN
fnp-5883	463	76	•	•	NOUN
fnp-5883	463	77	relatively	relatively	ADV
fnp-5883	463	78	accessible	accessible	ADJ
fnp-5883	463	79	,	,	PUNCT
fnp-5883	463	80	and	and	CCONJ
fnp-5883	463	81	low	low	ADJ
fnp-5883	463	82	cost	cost	NOUN
fnp-5883	463	83	•	•	NOUN
fnp-5883	463	84	relatively	relatively	ADV
fnp-5883	463	85	fast	fast	ADV
fnp-5883	463	86	cooling	cooling	NOUN
fnp-5883	463	87	,	,	PUNCT
fnp-5883	463	88	can	can	AUX
fnp-5883	463	89	handle	handle	VERB
fnp-5883	463	90	large	large	ADJ
fnp-5883	463	91	samples	sample	NOUN
fnp-5883	463	92	•	•	NOUN
fnp-5883	463	93	can	can	AUX
fnp-5883	463	94	still	still	ADV
fnp-5883	463	95	produce	produce	VERB
fnp-5883	463	96	significant	significant	ADJ
fnp-5883	463	97	ice	ice	NOUN
fnp-5883	463	98	artifacts	artifact	NOUN
fnp-5883	463	99	•	•	NOUN
fnp-5883	463	100	cooling	cool	VERB
fnp-5883	463	101	rate	rate	NOUN
fnp-5883	463	102	inconsistent	inconsistent	NOUN
fnp-5883	463	103	based	base	VERB
fnp-5883	463	104	on	on	ADP
fnp-5883	463	105	contact	contact	NOUN
fnp-5883	463	106	with	with	ADP
fnp-5883	463	107	dry	dry	ADJ
fnp-5883	463	108	ice	ice	NOUN
fnp-5883	463	109	freezing	freezing	NOUN
fnp-5883	463	110	between	between	ADP
fnp-5883	463	111	cooled	cool	VERB
fnp-5883	463	112	metal	metal	NOUN
fnp-5883	463	113	plates	plate	NOUN
fnp-5883	463	114	•	•	ADV
fnp-5883	463	115	practical	practical	ADJ
fnp-5883	463	116	for	for	ADP
fnp-5883	463	117	large	large	ADJ
fnp-5883	463	118	but	but	CCONJ
fnp-5883	463	119	relatively	relatively	ADV
fnp-5883	463	120	thin	thin	ADJ
fnp-5883	463	121	brain	brain	NOUN
fnp-5883	463	122	slices	slice	NOUN
fnp-5883	463	123	•	•	ADP
fnp-5883	463	124	relatively	relatively	ADV
fnp-5883	463	125	rapid	rapid	ADJ
fnp-5883	463	126	cooling	cooling	NOUN
fnp-5883	463	127	,	,	PUNCT
fnp-5883	463	128	and	and	CCONJ
fnp-5883	463	129	widely	widely	ADV
fnp-5883	463	130	used	use	VERB
fnp-5883	463	131	in	in	ADP
fnp-5883	463	132	brain	brain	NOUN
fnp-5883	463	133	banks	bank	NOUN
fnp-5883	463	134	•	•	ADV
fnp-5883	463	135	cooling	cool	VERB
fnp-5883	463	136	rate	rate	NOUN
fnp-5883	463	137	decreases	decrease	NOUN
fnp-5883	463	138	with	with	ADP
fnp-5883	463	139	depth	depth	NOUN
fnp-5883	463	140	•	•	NOUN
fnp-5883	463	141	damage	damage	NOUN
fnp-5883	463	142	at	at	ADP
fnp-5883	463	143	cut	cut	VERB
fnp-5883	463	144	interfaces	interface	NOUN
fnp-5883	463	145	•	•	ADV
fnp-5883	463	146	not	not	PART
fnp-5883	463	147	suitable	suitable	ADJ
fnp-5883	463	148	for	for	ADP
fnp-5883	463	149	preservation	preservation	NOUN
fnp-5883	463	150	of	of	ADP
fnp-5883	463	151	whole	whole	ADJ
fnp-5883	463	152	brains	brain	NOUN
fnp-5883	463	153	of	of	ADP
fnp-5883	463	154	larger	large	ADJ
fnp-5883	463	155	mammals	mammal	NOUN
fnp-5883	463	156	freezing	freeze	VERB
fnp-5883	463	157	in	in	ADP
fnp-5883	463	158	liquid	liquid	ADJ
fnp-5883	463	159	nitrogen	nitrogen	NOUN
fnp-5883	463	160	vapor	vapor	NOUN
fnp-5883	463	161	•	•	ADV
fnp-5883	463	162	relatively	relatively	ADV
fnp-5883	463	163	fast	fast	ADJ
fnp-5883	463	164	cooling	cool	VERB
fnp-5883	463	165	rate	rate	NOUN
fnp-5883	463	166	and	and	CCONJ
fnp-5883	463	167	very	very	ADV
fnp-5883	463	168	low	low	ADJ
fnp-5883	463	169	storage	storage	NOUN
fnp-5883	463	170	temperature	temperature	NOUN
fnp-5883	463	171	achievable	achievable	ADJ
fnp-5883	463	172	•	•	ADV
fnp-5883	463	173	less	less	ADV
fnp-5883	463	174	likely	likely	ADJ
fnp-5883	463	175	to	to	PART
fnp-5883	463	176	cause	cause	VERB
fnp-5883	463	177	tissue	tissue	NOUN
fnp-5883	463	178	cracking	cracking	NOUN
fnp-5883	463	179	than	than	ADP
fnp-5883	463	180	liquid	liquid	ADJ
fnp-5883	463	181	immersion	immersion	NOUN
fnp-5883	463	182	•	•	NOUN
fnp-5883	463	183	cooling	cool	VERB
fnp-5883	463	184	rate	rate	NOUN
fnp-5883	463	185	may	may	AUX
fnp-5883	463	186	still	still	ADV
fnp-5883	463	187	be	be	AUX
fnp-5883	463	188	too	too	ADV
fnp-5883	463	189	slow	slow	ADJ
fnp-5883	463	190	for	for	ADP
fnp-5883	463	191	optimal	optimal	ADJ
fnp-5883	463	192	preservation	preservation	NOUN
fnp-5883	463	193	•	•	NUM
fnp-5883	463	194	requires	require	VERB
fnp-5883	463	195	specialized	specialized	ADJ
fnp-5883	463	196	equipment	equipment	NOUN
fnp-5883	463	197	•	•	NOUN
fnp-5883	463	198	potential	potential	ADJ
fnp-5883	463	199	safety	safety	NOUN
fnp-5883	463	200	hazards	hazard	NOUN
fnp-5883	463	201	with	with	ADP
fnp-5883	463	202	liquid	liquid	ADJ
fnp-5883	463	203	nitrogen	nitrogen	NOUN
fnp-5883	463	204	handling	handling	NOUN
fnp-5883	463	205	freezing	freezing	NOUN
fnp-5883	463	206	in	in	ADP
fnp-5883	463	207	cooled	cool	VERB
fnp-5883	463	208	isopentane	isopentane	NOUN
fnp-5883	463	209	•	•	ADP
fnp-5883	463	210	rapid	rapid	ADJ
fnp-5883	463	211	,	,	PUNCT
fnp-5883	463	212	uniform	uniform	ADJ
fnp-5883	463	213	cooling	cool	VERB
fnp-5883	463	214	along	along	ADP
fnp-5883	463	215	surface	surface	NOUN
fnp-5883	463	216	inwards	inward	NOUN
fnp-5883	463	217	•	•	ADV
fnp-5883	463	218	avoids	avoid	VERB
fnp-5883	463	219	leidenfrost	leidenfrost	ADJ
fnp-5883	463	220	effect	effect	NOUN
fnp-5883	463	221	seen	see	VERB
fnp-5883	463	222	with	with	ADP
fnp-5883	463	223	liquid	liquid	ADJ
fnp-5883	463	224	nitrogen	nitrogen	NOUN
fnp-5883	463	225	immersion	immersion	NOUN
fnp-5883	463	226	•	•	ADV
fnp-5883	463	227	excellent	excellent	ADJ
fnp-5883	463	228	for	for	ADP
fnp-5883	463	229	preservation	preservation	NOUN
fnp-5883	463	230	of	of	ADP
fnp-5883	463	231	small	small	ADJ
fnp-5883	463	232	brain	brain	NOUN
fnp-5883	463	233	samples	sample	NOUN
fnp-5883	463	234	,	,	PUNCT
fnp-5883	463	235	as	as	SCONJ
fnp-5883	463	236	seen	see	VERB
fnp-5883	463	237	on	on	ADP
fnp-5883	463	238	light	light	ADJ
fnp-5883	463	239	microscopy	microscopy	NOUN
fnp-5883	463	240	•	•	NOUN
fnp-5883	463	241	requires	require	VERB
fnp-5883	463	242	careful	careful	ADJ
fnp-5883	463	243	handling	handling	NOUN
fnp-5883	463	244	of	of	ADP
fnp-5883	463	245	isopentane	isopentane	NOUN
fnp-5883	463	246	•	•	NUM
fnp-5883	463	247	may	may	AUX
fnp-5883	463	248	cause	cause	VERB
fnp-5883	463	249	tissue	tissue	NOUN
fnp-5883	463	250	cracking	crack	VERB
fnp-5883	463	251	in	in	ADP
fnp-5883	463	252	large	large	ADJ
fnp-5883	463	253	samples	sample	NOUN
fnp-5883	463	254	•	•	ADV
fnp-5883	463	255	not	not	PART
fnp-5883	463	256	widely	widely	ADV
fnp-5883	463	257	tested	test	VERB
fnp-5883	463	258	on	on	ADP
fnp-5883	463	259	large	large	ADJ
fnp-5883	463	260	samples	sample	NOUN
fnp-5883	463	261	,	,	PUNCT
fnp-5883	463	262	still	still	ADV
fnp-5883	463	263	limited	limit	VERB
fnp-5883	463	264	by	by	ADP
fnp-5883	463	265	depth	depth	NOUN
fnp-5883	463	266	cryopreservation	cryopreservation	NOUN
fnp-5883	463	267	by	by	ADP
fnp-5883	463	268	immersion	immersion	NOUN
fnp-5883	463	269	in	in	ADP
fnp-5883	463	270	cryoprotectants	cryoprotectant	NOUN
fnp-5883	463	271	•	•	NUM
fnp-5883	463	272	reduces	reduce	VERB
fnp-5883	463	273	ice	ice	NOUN
fnp-5883	463	274	crystal	crystal	NOUN
fnp-5883	463	275	formation	formation	NOUN
fnp-5883	463	276	and	and	CCONJ
fnp-5883	463	277	improves	improve	VERB
fnp-5883	463	278	preservation	preservation	NOUN
fnp-5883	463	279	of	of	ADP
fnp-5883	463	280	cellular	cellular	ADJ
fnp-5883	463	281	structures	structure	NOUN
fnp-5883	463	282	•	•	NOUN
fnp-5883	463	283	potential	potential	NOUN
fnp-5883	463	284	for	for	ADP
fnp-5883	463	285	vitrification	vitrification	NOUN
fnp-5883	463	286	in	in	ADP
fnp-5883	463	287	thin	thin	ADJ
fnp-5883	463	288	samples	sample	NOUN
fnp-5883	463	289	•	•	ADP
fnp-5883	463	290	slow	slow	ADJ
fnp-5883	463	291	penetration	penetration	NOUN
fnp-5883	463	292	rate	rate	NOUN
fnp-5883	463	293	makes	make	VERB
fnp-5883	463	294	larger	large	ADJ
fnp-5883	463	295	samples	sample	NOUN
fnp-5883	463	296	challenging	challenge	VERB
fnp-5883	463	297	•	•	NUM
fnp-5883	463	298	cryoprotectants	cryoprotectant	NOUN
fnp-5883	463	299	can	can	AUX
fnp-5883	463	300	cause	cause	VERB
fnp-5883	463	301	osmotic	osmotic	ADJ
fnp-5883	463	302	stress	stress	NOUN
fnp-5883	463	303	,	,	PUNCT
fnp-5883	463	304	which	which	PRON
fnp-5883	463	305	may	may	AUX
fnp-5883	463	306	alter	alter	VERB
fnp-5883	463	307	tissue	tissue	NOUN
fnp-5883	463	308	ultrastructure	ultrastructure	NOUN
fnp-5883	463	309	cryopreservation	cryopreservation	NOUN
fnp-5883	463	310	by	by	ADP
fnp-5883	463	311	perfusion	perfusion	NOUN
fnp-5883	463	312	with	with	ADP
fnp-5883	463	313	cryoprotectants	cryoprotectant	NOUN
fnp-5883	463	314	•	•	ADV
fnp-5883	463	315	more	more	ADV
fnp-5883	463	316	uniform	uniform	ADJ
fnp-5883	463	317	distribution	distribution	NOUN
fnp-5883	463	318	of	of	ADP
fnp-5883	463	319	cryoprotectants	cryoprotectant	NOUN
fnp-5883	463	320	•	•	NOUN
fnp-5883	463	321	potential	potential	NOUN
fnp-5883	463	322	for	for	ADP
fnp-5883	463	323	better	well	ADJ
fnp-5883	463	324	preservation	preservation	NOUN
fnp-5883	463	325	in	in	ADP
fnp-5883	463	326	larger	large	ADJ
fnp-5883	463	327	samples	sample	NOUN
fnp-5883	463	328	,	,	PUNCT
fnp-5883	463	329	with	with	ADP
fnp-5883	463	330	vitrification	vitrification	NOUN
fnp-5883	463	331	being	be	AUX
fnp-5883	463	332	also	also	ADV
fnp-5883	463	333	theoretically	theoretically	ADV
fnp-5883	463	334	possible	possible	ADJ
fnp-5883	463	335	•	•	ADP
fnp-5883	463	336	challenging	challenge	VERB
fnp-5883	463	337	to	to	PART
fnp-5883	463	338	perform	perform	VERB
fnp-5883	463	339	,	,	PUNCT
fnp-5883	463	340	especially	especially	ADV
fnp-5883	463	341	postmortem	postmortem	NOUN
fnp-5883	463	342	.	.	PUNCT
fnp-5883	464	1	not	not	PART
fnp-5883	464	2	widely	widely	ADV
fnp-5883	464	3	performed	perform	VERB
fnp-5883	464	4	•	•	NUM
fnp-5883	464	5	risk	risk	NOUN
fnp-5883	464	6	of	of	ADP
fnp-5883	464	7	incomplete	incomplete	ADJ
fnp-5883	464	8	perfusion	perfusion	NOUN
fnp-5883	464	9	•	•	ADP
fnp-5883	464	10	time	time	NOUN
fnp-5883	464	11	-	-	PUNCT
fnp-5883	464	12	consuming	consume	VERB
fnp-5883	464	13	process	process	NOUN
fnp-5883	464	14	with	with	ADP
fnp-5883	464	15	risk	risk	NOUN
fnp-5883	464	16	of	of	ADP
fnp-5883	464	17	cellular	cellular	ADJ
fnp-5883	464	18	decomposition	decomposition	NOUN
fnp-5883	464	19	table	table	NOUN
fnp-5883	464	20	 	 	SPACE
fnp-5883	464	21	6	6	NUM
fnp-5883	464	22	.	.	PUNCT
fnp-5883	465	1	summary	summary	NOUN
fnp-5883	465	2	of	of	ADP
fnp-5883	465	3	methods	method	NOUN
fnp-5883	465	4	for	for	ADP
fnp-5883	465	5	cryopreserving	cryopreserve	VERB
fnp-5883	465	6	brain	brain	NOUN
fnp-5883	465	7	tissue	tissue	NOUN
fnp-5883	465	8	.	.	PUNCT
fnp-5883	466	1	several	several	ADJ
fnp-5883	466	2	approaches	approach	NOUN
fnp-5883	466	3	for	for	ADP
fnp-5883	466	4	cryopreserving	cryopreserve	VERB
fnp-5883	466	5	brain	brain	NOUN
fnp-5883	466	6	tissue	tissue	NOUN
fnp-5883	466	7	are	be	AUX
fnp-5883	466	8	described	describe	VERB
fnp-5883	466	9	,	,	PUNCT
fnp-5883	466	10	alongside	alongside	ADP
fnp-5883	466	11	their	their	PRON
fnp-5883	466	12	relative	relative	ADJ
fnp-5883	466	13	benefits	benefit	NOUN
fnp-5883	466	14	and	and	CCONJ
fnp-5883	466	15	drawbacks	drawback	NOUN
fnp-5883	466	16	.	.	PUNCT
fnp-5883	467	1	implications	implication	NOUN
fnp-5883	467	2	for	for	ADP
fnp-5883	467	3	brain	brain	NOUN
fnp-5883	467	4	banking	banking	NOUN
fnp-5883	467	5	brain	brain	NOUN
fnp-5883	467	6	banks	bank	NOUN
fnp-5883	467	7	may	may	AUX
fnp-5883	467	8	have	have	VERB
fnp-5883	467	9	different	different	ADJ
fnp-5883	467	10	needs	need	NOUN
fnp-5883	467	11	for	for	ADP
fnp-5883	467	12	their	their	PRON
fnp-5883	467	13	cryopreservation	cryopreservation	NOUN
fnp-5883	467	14	methods	method	NOUN
fnp-5883	467	15	depending	depend	VERB
fnp-5883	467	16	upon	upon	SCONJ
fnp-5883	467	17	the	the	DET
fnp-5883	467	18	intended	intend	VERB
fnp-5883	467	19	use	use	NOUN
fnp-5883	467	20	of	of	ADP
fnp-5883	467	21	their	their	PRON
fnp-5883	467	22	tissue	tissue	NOUN
fnp-5883	467	23	samples	sample	NOUN
fnp-5883	467	24	(	(	PUNCT
fnp-5883	467	25	table	table	NOUN
fnp-5883	467	26	 	 	SPACE
fnp-5883	467	27	7	7	NUM
fnp-5883	467	28	)	)	PUNCT
fnp-5883	467	29	.	.	PUNCT
fnp-5883	468	1	for	for	ADP
fnp-5883	468	2	whole	whole	ADJ
fnp-5883	468	3	brain	brain	NOUN
fnp-5883	468	4	slices	slice	NOUN
fnp-5883	468	5	,	,	PUNCT
fnp-5883	468	6	which	which	PRON
fnp-5883	468	7	are	be	AUX
fnp-5883	468	8	commonly	commonly	ADV
fnp-5883	468	9	used	use	VERB
fnp-5883	468	10	in	in	ADP
fnp-5883	468	11	brain	brain	NOUN
fnp-5883	468	12	banking	banking	NOUN
fnp-5883	468	13	,	,	PUNCT
fnp-5883	468	14	preservation	preservation	NOUN
fnp-5883	468	15	of	of	ADP
fnp-5883	468	16	overall	overall	ADJ
fnp-5883	468	17	tissue	tissue	NOUN
fnp-5883	468	18	architecture	architecture	NOUN
fnp-5883	468	19	is	be	AUX
fnp-5883	468	20	often	often	ADV
fnp-5883	468	21	a	a	DET
fnp-5883	468	22	primary	primary	ADJ
fnp-5883	468	23	concern	concern	NOUN
fnp-5883	468	24	.	.	PUNCT
fnp-5883	469	1	in	in	ADP
fnp-5883	469	2	this	this	DET
fnp-5883	469	3	case	case	NOUN
fnp-5883	469	4	,	,	PUNCT
fnp-5883	469	5	freezing	freeze	VERB
fnp-5883	469	6	the	the	DET
fnp-5883	469	7	slices	slice	NOUN
fnp-5883	469	8	between	between	ADP
fnp-5883	469	9	cooled	cool	VERB
fnp-5883	469	10	metal	metal	NOUN
fnp-5883	469	11	plates	plate	NOUN
fnp-5883	469	12	offers	offer	VERB
fnp-5883	469	13	a	a	DET
fnp-5883	469	14	relatively	relatively	ADV
fnp-5883	469	15	simple	simple	ADJ
fnp-5883	469	16	and	and	CCONJ
fnp-5883	469	17	effective	effective	ADJ
fnp-5883	469	18	approach	approach	NOUN
fnp-5883	469	19	.	.	PUNCT
fnp-5883	470	1	for	for	ADP
fnp-5883	470	2	smaller	small	ADJ
fnp-5883	470	3	tissue	tissue	NOUN
fnp-5883	470	4	fragments	fragment	NOUN
fnp-5883	470	5	,	,	PUNCT
fnp-5883	470	6	where	where	SCONJ
fnp-5883	470	7	preservation	preservation	NOUN
fnp-5883	470	8	of	of	ADP
fnp-5883	470	9	cell	cell	NOUN
fnp-5883	470	10	membrane	membrane	NOUN
fnp-5883	470	11	morphology	morphology	NOUN
fnp-5883	470	12	as	as	SCONJ
fnp-5883	470	13	seen	see	VERB
fnp-5883	470	14	on	on	ADP
fnp-5883	470	15	light	light	ADJ
fnp-5883	470	16	microscopy	microscopy	NOUN
fnp-5883	470	17	is	be	AUX
fnp-5883	470	18	desired	desire	VERB
fnp-5883	470	19	,	,	PUNCT
fnp-5883	470	20	rapid	rapid	ADJ
fnp-5883	470	21	freezing	freezing	NOUN
fnp-5883	470	22	methods	method	NOUN
fnp-5883	470	23	such	such	ADJ
fnp-5883	470	24	as	as	ADP
fnp-5883	470	25	immersion	immersion	NOUN
fnp-5883	470	26	in	in	ADP
fnp-5883	470	27	liquid	liquid	ADJ
fnp-5883	470	28	nitrogen	nitrogen	NOUN
fnp-5883	470	29	vapor	vapor	NOUN
fnp-5883	470	30	or	or	CCONJ
fnp-5883	470	31	liquid	liquid	ADJ
fnp-5883	470	32	nitrogen	nitrogen	NOUN
fnp-5883	470	33	-	-	PUNCT
fnp-5883	470	34	cooled	cool	VERB
fnp-5883	470	35	isopentane	isopentane	NOUN
fnp-5883	470	36	can	can	AUX
fnp-5883	470	37	be	be	AUX
fnp-5883	470	38	used	use	VERB
fnp-5883	470	39	.	.	PUNCT
fnp-5883	471	1	however	however	ADV
fnp-5883	471	2	,	,	PUNCT
fnp-5883	471	3	the	the	DET
fnp-5883	471	4	effectiveness	effectiveness	NOUN
fnp-5883	471	5	of	of	ADP
fnp-5883	471	6	these	these	DET
fnp-5883	471	7	methods	method	NOUN
fnp-5883	471	8	for	for	ADP
fnp-5883	471	9	preserving	preserve	VERB
fnp-5883	471	10	delicate	delicate	ADJ
fnp-5883	471	11	cellular	cellular	ADJ
fnp-5883	471	12	structures	structure	NOUN
fnp-5883	471	13	can	can	AUX
fnp-5883	471	14	vary	vary	VERB
fnp-5883	471	15	depending	depend	VERB
fnp-5883	471	16	on	on	ADP
fnp-5883	471	17	factors	factor	NOUN
fnp-5883	471	18	such	such	ADJ
fnp-5883	471	19	as	as	ADP
fnp-5883	471	20	tissue	tissue	NOUN
fnp-5883	471	21	type	type	NOUN
fnp-5883	471	22	and	and	CCONJ
fnp-5883	471	23	freezing	freezing	NOUN
fnp-5883	471	24	rate	rate	NOUN
fnp-5883	471	25	.	.	PUNCT
fnp-5883	472	1	if	if	SCONJ
fnp-5883	472	2	one	one	PRON
fnp-5883	472	3	has	have	VERB
fnp-5883	472	4	access	access	NOUN
fnp-5883	472	5	to	to	ADP
fnp-5883	472	6	specialized	specialized	ADJ
fnp-5883	472	7	equipment	equipment	NOUN
fnp-5883	472	8	and	and	CCONJ
fnp-5883	472	9	expertise	expertise	NOUN
fnp-5883	472	10	,	,	PUNCT
fnp-5883	472	11	then	then	ADV
fnp-5883	472	12	cryofixation	cryofixation	NOUN
fnp-5883	472	13	could	could	AUX
fnp-5883	472	14	potentially	potentially	ADV
fnp-5883	472	15	be	be	AUX
fnp-5883	472	16	used	use	VERB
fnp-5883	472	17	on	on	ADP
fnp-5883	472	18	very	very	ADV
fnp-5883	472	19	thin	thin	ADJ
fnp-5883	472	20	sections	section	NOUN
fnp-5883	472	21	from	from	ADP
fnp-5883	472	22	small	small	ADJ
fnp-5883	472	23	biopsy	biopsy	NOUN
fnp-5883	472	24	specimens	specimen	NOUN
fnp-5883	472	25	to	to	PART
fnp-5883	472	26	achieve	achieve	VERB
fnp-5883	472	27	high	high	ADJ
fnp-5883	472	28	-	-	PUNCT
fnp-5883	472	29	quality	quality	NOUN
fnp-5883	472	30	preservation	preservation	NOUN
fnp-5883	472	31	of	of	ADP
fnp-5883	472	32	subcellular	subcellular	ADJ
fnp-5883	472	33	structures	structure	NOUN
fnp-5883	472	34	such	such	ADJ
fnp-5883	472	35	as	as	ADP
fnp-5883	472	36	synaptic	synaptic	ADJ
fnp-5883	472	37	vesicles	vesicle	NOUN
fnp-5883	472	38	.	.	PUNCT
fnp-5883	473	1	for	for	ADP
fnp-5883	473	2	those	those	PRON
fnp-5883	473	3	more	more	ADV
fnp-5883	473	4	interested	interested	ADJ
fnp-5883	473	5	in	in	ADP
fnp-5883	473	6	preserving	preserve	VERB
fnp-5883	473	7	the	the	DET
fnp-5883	473	8	biomolecular	biomolecular	ADJ
fnp-5883	473	9	properties	property	NOUN
fnp-5883	473	10	of	of	ADP
fnp-5883	473	11	the	the	DET
fnp-5883	473	12	tissue	tissue	NOUN
fnp-5883	473	13	and	and	CCONJ
fnp-5883	473	14	not	not	PART
fnp-5883	473	15	as	as	ADV
fnp-5883	473	16	much	much	ADJ
fnp-5883	473	17	in	in	ADP
fnp-5883	473	18	detailed	detailed	ADJ
fnp-5883	473	19	histological	histological	ADJ
fnp-5883	473	20	preservation	preservation	NOUN
fnp-5883	473	21	,	,	PUNCT
fnp-5883	473	22	the	the	DET
fnp-5883	473	23	choice	choice	NOUN
fnp-5883	473	24	of	of	ADP
fnp-5883	473	25	freezing	freeze	VERB
fnp-5883	473	26	method	method	NOUN
fnp-5883	473	27	is	be	AUX
fnp-5883	473	28	less	less	ADV
fnp-5883	473	29	critical	critical	ADJ
fnp-5883	473	30	.	.	PUNCT
fnp-5883	474	1	in	in	ADP
fnp-5883	474	2	this	this	DET
fnp-5883	474	3	case	case	NOUN
fnp-5883	474	4	,	,	PUNCT
fnp-5883	474	5	simpler	simple	ADJ
fnp-5883	474	6	methods	method	NOUN
fnp-5883	474	7	,	,	PUNCT
fnp-5883	474	8	such	such	ADJ
fnp-5883	474	9	as	as	ADP
fnp-5883	474	10	placement	placement	NOUN
fnp-5883	474	11	in	in	ADP
fnp-5883	474	12	dry	dry	ADJ
fnp-5883	474	13	ice	ice	NOUN
fnp-5883	474	14	or	or	CCONJ
fnp-5883	474	15	in	in	ADP
fnp-5883	474	16	an	an	DET
fnp-5883	474	17	ultra	ultra	ADJ
fnp-5883	474	18	-	-	ADJ
fnp-5883	474	19	low	low	ADJ
fnp-5883	474	20	temperature	temperature	NOUN
fnp-5883	474	21	(	(	PUNCT
fnp-5883	474	22	‑80	‑80	NOUN
fnp-5883	474	23	 	 	SPACE
fnp-5883	474	24	°	°	ADP
fnp-5883	474	25	c	c	NOUN
fnp-5883	474	26	)	)	PUNCT
fnp-5883	474	27	freezer	freezer	NOUN
fnp-5883	474	28	,	,	PUNCT
fnp-5883	474	29	might	might	AUX
fnp-5883	474	30	be	be	AUX
fnp-5883	474	31	sufficient	sufficient	ADJ
fnp-5883	474	32	.	.	PUNCT
fnp-5883	475	1	finally	finally	ADV
fnp-5883	475	2	,	,	PUNCT
fnp-5883	475	3	for	for	ADP
fnp-5883	475	4	specialized	specialized	ADJ
fnp-5883	475	5	research	research	NOUN
fnp-5883	475	6	applications	application	NOUN
fnp-5883	475	7	,	,	PUNCT
fnp-5883	475	8	such	such	ADJ
fnp-5883	475	9	as	as	ADP
fnp-5883	475	10	those	those	PRON
fnp-5883	475	11	interested	interested	ADJ
fnp-5883	475	12	in	in	ADP
fnp-5883	475	13	the	the	DET
fnp-5883	475	14	preservation	preservation	NOUN
fnp-5883	475	15	of	of	ADP
fnp-5883	475	16	electrophysiological	electrophysiological	ADJ
fnp-5883	475	17	features	feature	NOUN
fnp-5883	475	18	of	of	ADP
fnp-5883	475	19	fresh	fresh	ADJ
fnp-5883	475	20	tissue	tissue	NOUN
fnp-5883	475	21	slices	slice	NOUN
fnp-5883	475	22	(	(	PUNCT
fnp-5883	475	23	german	german	NOUN
fnp-5883	475	24	and	and	CCONJ
fnp-5883	475	25	akdaş	akdaş	NOUN
fnp-5883	475	26	,	,	PUNCT
fnp-5883	475	27	2024	2024	NUM
fnp-5883	475	28	)	)	PUNCT
fnp-5883	475	29	,	,	PUNCT
fnp-5883	475	30	the	the	DET
fnp-5883	475	31	use	use	NOUN
fnp-5883	475	32	of	of	ADP
fnp-5883	475	33	cryoprotective	cryoprotective	ADJ
fnp-5883	475	34	agents	agent	NOUN
fnp-5883	475	35	,	,	PUNCT
fnp-5883	475	36	while	while	SCONJ
fnp-5883	475	37	a	a	DET
fnp-5883	475	38	relatively	relatively	ADV
fnp-5883	475	39	complex	complex	ADJ
fnp-5883	475	40	procedure	procedure	NOUN
fnp-5883	475	41	,	,	PUNCT
fnp-5883	475	42	might	might	AUX
fnp-5883	475	43	be	be	AUX
fnp-5883	475	44	justified	justify	VERB
fnp-5883	475	45	.	.	PUNCT
fnp-5883	476	1	because	because	SCONJ
fnp-5883	476	2	cryopreservation	cryopreservation	NOUN
fnp-5883	476	3	can	can	AUX
fnp-5883	476	4	be	be	AUX
fnp-5883	476	5	expensive	expensive	ADJ
fnp-5883	476	6	and	and	CCONJ
fnp-5883	476	7	highly	highly	ADV
fnp-5883	476	8	labor	labor	NOUN
fnp-5883	476	9	-	-	PUNCT
fnp-5883	476	10	intensive	intensive	ADJ
fnp-5883	476	11	,	,	PUNCT
fnp-5883	476	12	it	it	PRON
fnp-5883	476	13	is	be	AUX
fnp-5883	476	14	essential	essential	ADJ
fnp-5883	476	15	to	to	PART
fnp-5883	476	16	be	be	AUX
fnp-5883	476	17	pragmatic	pragmatic	ADJ
fnp-5883	476	18	and	and	CCONJ
fnp-5883	476	19	to	to	PART
fnp-5883	476	20	choose	choose	VERB
fnp-5883	476	21	the	the	DET
fnp-5883	476	22	simplest	simple	ADJ
fnp-5883	476	23	method	method	NOUN
fnp-5883	476	24	that	that	PRON
fnp-5883	476	25	adequately	adequately	ADV
fnp-5883	476	26	meets	meet	VERB
fnp-5883	476	27	the	the	DET
fnp-5883	476	28	specific	specific	ADJ
fnp-5883	476	29	preservation	preservation	NOUN
fnp-5883	476	30	needs	need	NOUN
fnp-5883	476	31	of	of	ADP
fnp-5883	476	32	the	the	DET
fnp-5883	476	33	brain	brain	NOUN
fnp-5883	476	34	bank	bank	NOUN
fnp-5883	476	35	and	and	CCONJ
fnp-5883	476	36	its	its	PRON
fnp-5883	476	37	research	research	NOUN
fnp-5883	476	38	community	community	NOUN
fnp-5883	476	39	.	.	PUNCT
fnp-5883	477	1	this	this	DET
fnp-5883	477	2	approach	approach	NOUN
fnp-5883	477	3	ensures	ensure	VERB
fnp-5883	477	4	efficient	efficient	ADJ
fnp-5883	477	5	use	use	NOUN
fnp-5883	477	6	of	of	ADP
fnp-5883	477	7	resources	resource	NOUN
fnp-5883	477	8	while	while	SCONJ
fnp-5883	477	9	still	still	ADV
fnp-5883	477	10	maintaining	maintain	VERB
fnp-5883	477	11	the	the	DET
fnp-5883	477	12	quality	quality	NOUN
fnp-5883	477	13	necessary	necessary	ADJ
fnp-5883	477	14	for	for	ADP
fnp-5883	477	15	intended	intend	VERB
fnp-5883	477	16	downstream	downstream	ADJ
fnp-5883	477	17	applications	application	NOUN
fnp-5883	477	18	.	.	PUNCT
fnp-5883	478	1	tissue	tissue	NOUN
fnp-5883	478	2	type	type	NOUN
fnp-5883	478	3	preservation	preservation	NOUN
fnp-5883	478	4	goal	goal	NOUN
fnp-5883	478	5	recommended	recommend	VERB
fnp-5883	478	6	approach	approach	NOUN
fnp-5883	478	7	whole	whole	ADJ
fnp-5883	478	8	brain	brain	NOUN
fnp-5883	478	9	slices	slice	NOUN
fnp-5883	478	10	overall	overall	ADJ
fnp-5883	478	11	tissue	tissue	NOUN
fnp-5883	478	12	architecture	architecture	NOUN
fnp-5883	478	13	freezing	freezing	NOUN
fnp-5883	478	14	between	between	ADP
fnp-5883	478	15	cooled	cool	VERB
fnp-5883	478	16	metal	metal	NOUN
fnp-5883	478	17	plates	plate	NOUN
fnp-5883	478	18	small	small	ADJ
fnp-5883	478	19	tissue	tissue	NOUN
fnp-5883	478	20	fragments	fragment	VERB
fnp-5883	478	21	cell	cell	NOUN
fnp-5883	478	22	membrane	membrane	NOUN
fnp-5883	478	23	morphology	morphology	NOUN
fnp-5883	478	24	on	on	ADP
fnp-5883	478	25	light	light	ADJ
fnp-5883	478	26	microscopy	microscopy	NOUN
fnp-5883	478	27	snap	snap	NOUN
fnp-5883	478	28	freezing	freezing	NOUN
fnp-5883	478	29	in	in	ADP
fnp-5883	478	30	liquid	liquid	ADJ
fnp-5883	478	31	nitrogen	nitrogen	NOUN
fnp-5883	478	32	-	-	PUNCT
fnp-5883	478	33	cooled	cool	VERB
fnp-5883	478	34	isopentane	isopentane	NOUN
fnp-5883	478	35	very	very	ADV
fnp-5883	478	36	thin	thin	ADJ
fnp-5883	478	37	sections	section	NOUN
fnp-5883	478	38	subcellular	subcellular	ADJ
fnp-5883	478	39	structural	structural	ADJ
fnp-5883	478	40	features	feature	NOUN
fnp-5883	478	41	on	on	ADP
fnp-5883	478	42	electron	electron	NOUN
fnp-5883	478	43	microscopy	microscopy	NOUN
fnp-5883	478	44	cryofixation	cryofixation	NOUN
fnp-5883	478	45	,	,	PUNCT
fnp-5883	478	46	for	for	ADP
fnp-5883	478	47	example	example	NOUN
fnp-5883	478	48	via	via	ADP
fnp-5883	478	49	high	high	ADJ
fnp-5883	478	50	-	-	PUNCT
fnp-5883	478	51	pressure	pressure	NOUN
fnp-5883	478	52	freezing	freeze	VERB
fnp-5883	478	53	any	any	DET
fnp-5883	478	54	tissue	tissue	NOUN
fnp-5883	478	55	type	type	NOUN
fnp-5883	478	56	biomolecular	biomolecular	ADJ
fnp-5883	478	57	properties	property	NOUN
fnp-5883	478	58	(	(	PUNCT
fnp-5883	478	59	e.g.	e.g.	ADV
fnp-5883	478	60	,	,	PUNCT
fnp-5883	478	61	rna	rna	NOUN
fnp-5883	478	62	,	,	PUNCT
fnp-5883	478	63	proteins	protein	NOUN
fnp-5883	478	64	)	)	PUNCT
fnp-5883	478	65	multiple	multiple	ADJ
fnp-5883	478	66	options	option	NOUN
fnp-5883	478	67	,	,	PUNCT
fnp-5883	478	68	including	include	VERB
fnp-5883	478	69	placement	placement	NOUN
fnp-5883	478	70	in	in	ADP
fnp-5883	478	71	dry	dry	ADJ
fnp-5883	478	72	ice	ice	NOUN
fnp-5883	478	73	,	,	PUNCT
fnp-5883	478	74	or	or	CCONJ
fnp-5883	478	75	placement	placement	NOUN
fnp-5883	478	76	in	in	ADP
fnp-5883	478	77	a	a	DET
fnp-5883	478	78	‑80	‑80	NOUN
fnp-5883	478	79	 	 	SPACE
fnp-5883	478	80	°	°	ADP
fnp-5883	478	81	c	c	PROPN
fnp-5883	478	82	freezer	freezer	NOUN
fnp-5883	478	83	fresh	fresh	ADJ
fnp-5883	478	84	tissue	tissue	NOUN
fnp-5883	478	85	slices	slice	NOUN
fnp-5883	478	86	electrophysiological	electrophysiological	ADJ
fnp-5883	478	87	properties	property	NOUN
fnp-5883	478	88	immersion	immersion	NOUN
fnp-5883	478	89	of	of	ADP
fnp-5883	478	90	cryoprotective	cryoprotective	ADJ
fnp-5883	478	91	agents	agent	NOUN
fnp-5883	478	92	,	,	PUNCT
fnp-5883	478	93	or	or	CCONJ
fnp-5883	478	94	perfusion	perfusion	NOUN
fnp-5883	478	95	prior	prior	ADV
fnp-5883	478	96	to	to	ADP
fnp-5883	478	97	sectioning	section	VERB
fnp-5883	478	98	table	table	NOUN
fnp-5883	478	99	 	 	SPACE
fnp-5883	478	100	7	7	NUM
fnp-5883	478	101	.	.	PUNCT
fnp-5883	478	102	recommended	recommend	VERB
fnp-5883	478	103	cryopreservation	cryopreservation	NOUN
fnp-5883	478	104	methods	method	NOUN
fnp-5883	478	105	for	for	ADP
fnp-5883	478	106	several	several	ADJ
fnp-5883	478	107	different	different	ADJ
fnp-5883	478	108	brain	brain	NOUN
fnp-5883	478	109	banking	banking	NOUN
fnp-5883	478	110	goals	goal	NOUN
fnp-5883	478	111	and	and	CCONJ
fnp-5883	478	112	tissue	tissue	NOUN
fnp-5883	478	113	types	type	NOUN
fnp-5883	478	114	.	.	PUNCT
fnp-5883	479	1	we	we	PRON
fnp-5883	479	2	can	can	AUX
fnp-5883	479	3	also	also	ADV
fnp-5883	479	4	consider	consider	VERB
fnp-5883	479	5	the	the	DET
fnp-5883	479	6	perspective	perspective	NOUN
fnp-5883	479	7	of	of	ADP
fnp-5883	479	8	researchers	researcher	NOUN
fnp-5883	479	9	using	use	VERB
fnp-5883	479	10	existing	exist	VERB
fnp-5883	479	11	tissue	tissue	NOUN
fnp-5883	479	12	banked	bank	VERB
fnp-5883	479	13	at	at	ADP
fnp-5883	479	14	brain	brain	NOUN
fnp-5883	479	15	banks	bank	NOUN
fnp-5883	479	16	.	.	PUNCT
fnp-5883	480	1	samples	sample	NOUN
fnp-5883	480	2	that	that	PRON
fnp-5883	480	3	are	be	AUX
fnp-5883	480	4	frozen	frozen	ADJ
fnp-5883	480	5	may	may	AUX
fnp-5883	480	6	have	have	VERB
fnp-5883	480	7	more	more	ADJ
fnp-5883	480	8	variable	variable	ADJ
fnp-5883	480	9	histologic	histologic	ADJ
fnp-5883	480	10	qualities	quality	NOUN
fnp-5883	480	11	than	than	SCONJ
fnp-5883	480	12	commonly	commonly	ADV
fnp-5883	480	13	appreciated	appreciate	VERB
fnp-5883	480	14	.	.	PUNCT
fnp-5883	481	1	for	for	ADP
fnp-5883	481	2	researchers	researcher	NOUN
fnp-5883	481	3	interested	interested	ADJ
fnp-5883	481	4	in	in	ADP
fnp-5883	481	5	histological	histological	ADJ
fnp-5883	481	6	features	feature	NOUN
fnp-5883	481	7	,	,	PUNCT
fnp-5883	481	8	it	it	PRON
fnp-5883	481	9	may	may	AUX
fnp-5883	481	10	be	be	AUX
fnp-5883	481	11	worthwhile	worthwhile	ADJ
fnp-5883	481	12	to	to	PART
fnp-5883	481	13	seek	seek	VERB
fnp-5883	481	14	tissue	tissue	NOUN
fnp-5883	481	15	that	that	PRON
fnp-5883	481	16	has	have	AUX
fnp-5883	481	17	been	be	AUX
fnp-5883	481	18	frozen	freeze	VERB
fnp-5883	481	19	in	in	ADP
fnp-5883	481	20	a	a	DET
fnp-5883	481	21	rapid	rapid	ADJ
fnp-5883	481	22	,	,	PUNCT
fnp-5883	481	23	well	well	ADV
fnp-5883	481	24	-	-	PUNCT
fnp-5883	481	25	controlled	control	VERB
fnp-5883	481	26	manner	manner	NOUN
fnp-5883	481	27	,	,	PUNCT
fnp-5883	481	28	rather	rather	ADV
fnp-5883	481	29	than	than	ADP
fnp-5883	481	30	assuming	assume	VERB
fnp-5883	481	31	that	that	SCONJ
fnp-5883	481	32	the	the	DET
fnp-5883	481	33	morphological	morphological	ADJ
fnp-5883	481	34	quality	quality	NOUN
fnp-5883	481	35	of	of	ADP
fnp-5883	481	36	all	all	DET
fnp-5883	481	37	frozen	frozen	ADJ
fnp-5883	481	38	tissue	tissue	NOUN
fnp-5883	481	39	will	will	AUX
fnp-5883	481	40	be	be	AUX
fnp-5883	481	41	identical	identical	ADJ
fnp-5883	481	42	.	.	PUNCT
fnp-5883	482	1	years	year	NOUN
fnp-5883	482	2	of	of	ADP
fnp-5883	482	3	storage	storage	NOUN
fnp-5883	482	4	at	at	ADP
fnp-5883	482	5	‑80	‑80	NOUN
fnp-5883	482	6	 	 	SPACE
fnp-5883	482	7	°	°	NOUN
fnp-5883	482	8	c	c	PROPN
fnp-5883	482	9	also	also	ADV
fnp-5883	482	10	has	have	VERB
fnp-5883	482	11	the	the	DET
fnp-5883	482	12	potential	potential	NOUN
fnp-5883	482	13	to	to	PART
fnp-5883	482	14	affect	affect	VERB
fnp-5883	482	15	tissue	tissue	NOUN
fnp-5883	482	16	quality	quality	NOUN
fnp-5883	482	17	.	.	PUNCT
fnp-5883	483	1	using	use	VERB
fnp-5883	483	2	rapid	rapid	ADJ
fnp-5883	483	3	thawing	thaw	VERB
fnp-5883	483	4	techniques	technique	NOUN
fnp-5883	483	5	and/or	and/or	CCONJ
fnp-5883	483	6	thawing	thaw	VERB
fnp-5883	483	7	in	in	ADP
fnp-5883	483	8	fixative	fixative	NOUN
fnp-5883	483	9	may	may	AUX
fnp-5883	483	10	facilitate	facilitate	VERB
fnp-5883	483	11	morphological	morphological	ADJ
fnp-5883	483	12	preservation	preservation	NOUN
fnp-5883	483	13	in	in	ADP
fnp-5883	483	14	research	research	NOUN
fnp-5883	483	15	applications	application	NOUN
fnp-5883	483	16	,	,	PUNCT
fnp-5883	483	17	although	although	SCONJ
fnp-5883	483	18	the	the	DET
fnp-5883	483	19	best	good	ADJ
fnp-5883	483	20	methods	method	NOUN
fnp-5883	483	21	for	for	ADP
fnp-5883	483	22	tissue	tissue	NOUN
fnp-5883	483	23	rewarming	rewarming	NOUN
fnp-5883	483	24	are	be	AUX
fnp-5883	483	25	still	still	ADV
fnp-5883	483	26	not	not	PART
fnp-5883	483	27	well	well	ADV
fnp-5883	483	28	established	establish	VERB
fnp-5883	483	29	.	.	PUNCT
fnp-5883	484	1	notably	notably	ADV
fnp-5883	484	2	,	,	PUNCT
fnp-5883	484	3	preservation	preservation	NOUN
fnp-5883	484	4	of	of	ADP
fnp-5883	484	5	histologic	histologic	ADJ
fnp-5883	484	6	features	feature	NOUN
fnp-5883	484	7	is	be	AUX
fnp-5883	484	8	generally	generally	ADV
fnp-5883	484	9	expected	expect	VERB
fnp-5883	484	10	to	to	PART
fnp-5883	484	11	be	be	AUX
fnp-5883	484	12	superior	superior	ADJ
fnp-5883	484	13	in	in	ADP
fnp-5883	484	14	banked	banked	ADJ
fnp-5883	484	15	tissue	tissue	NOUN
fnp-5883	484	16	that	that	PRON
fnp-5883	484	17	has	have	AUX
fnp-5883	484	18	been	be	AUX
fnp-5883	484	19	chemically	chemically	ADV
fnp-5883	484	20	fixed	fix	VERB
fnp-5883	484	21	.	.	PUNCT
fnp-5883	485	1	therefore	therefore	ADV
fnp-5883	485	2	,	,	PUNCT
fnp-5883	485	3	there	there	PRON
fnp-5883	485	4	is	be	VERB
fnp-5883	485	5	currently	currently	ADV
fnp-5883	485	6	a	a	DET
fnp-5883	485	7	trade	trade	NOUN
fnp-5883	485	8	-	-	PUNCT
fnp-5883	485	9	off	off	NOUN
fnp-5883	485	10	between	between	ADP
fnp-5883	485	11	the	the	DET
fnp-5883	485	12	types	type	NOUN
fnp-5883	485	13	of	of	ADP
fnp-5883	485	14	tissue	tissue	NOUN
fnp-5883	485	15	generally	generally	ADV
fnp-5883	485	16	available	available	ADJ
fnp-5883	485	17	from	from	ADP
fnp-5883	485	18	brain	brain	NOUN
fnp-5883	485	19	banks	bank	NOUN
fnp-5883	485	20	,	,	PUNCT
fnp-5883	485	21	with	with	ADP
fnp-5883	485	22	frozen	frozen	ADJ
fnp-5883	485	23	samples	sample	NOUN
fnp-5883	485	24	allowing	allow	VERB
fnp-5883	485	25	certain	certain	ADJ
fnp-5883	485	26	molecular	molecular	ADJ
fnp-5883	485	27	biology	biology	NOUN
fnp-5883	485	28	studies	study	NOUN
fnp-5883	485	29	to	to	PART
fnp-5883	485	30	be	be	AUX
fnp-5883	485	31	more	more	ADV
fnp-5883	485	32	easily	easily	ADV
fnp-5883	485	33	performed	perform	VERB
fnp-5883	485	34	,	,	PUNCT
fnp-5883	485	35	and	and	CCONJ
fnp-5883	485	36	fixed	fix	VERB
fnp-5883	485	37	samples	sample	NOUN
fnp-5883	485	38	expected	expect	VERB
fnp-5883	485	39	to	to	PART
fnp-5883	485	40	have	have	VERB
fnp-5883	485	41	better	well	ADJ
fnp-5883	485	42	morphological	morphological	ADJ
fnp-5883	485	43	preservation	preservation	NOUN
fnp-5883	485	44	.	.	PUNCT
fnp-5883	486	1	areas	area	NOUN
fnp-5883	486	2	for	for	ADP
fnp-5883	486	3	further	further	ADJ
fnp-5883	486	4	research	research	NOUN
fnp-5883	486	5	some	some	DET
fnp-5883	486	6	research	research	NOUN
fnp-5883	486	7	applications	application	NOUN
fnp-5883	486	8	,	,	PUNCT
fnp-5883	486	9	such	such	ADJ
fnp-5883	486	10	as	as	ADP
fnp-5883	486	11	long	long	ADJ
fnp-5883	486	12	-	-	PUNCT
fnp-5883	486	13	distance	distance	NOUN
fnp-5883	486	14	circuit	circuit	NOUN
fnp-5883	486	15	mapping	mapping	NOUN
fnp-5883	486	16	and	and	CCONJ
fnp-5883	486	17	also	also	ADV
fnp-5883	486	18	biochemical	biochemical	ADJ
fnp-5883	486	19	assays	assay	NOUN
fnp-5883	486	20	,	,	PUNCT
fnp-5883	486	21	could	could	AUX
fnp-5883	486	22	potentially	potentially	ADV
fnp-5883	486	23	benefit	benefit	VERB
fnp-5883	486	24	from	from	ADP
fnp-5883	486	25	the	the	DET
fnp-5883	486	26	ability	ability	NOUN
fnp-5883	486	27	to	to	PART
fnp-5883	486	28	cryopreserve	cryopreserve	VERB
fnp-5883	486	29	an	an	DET
fnp-5883	486	30	entire	entire	ADJ
fnp-5883	486	31	brain	brain	NOUN
fnp-5883	486	32	while	while	SCONJ
fnp-5883	486	33	maintaining	maintain	VERB
fnp-5883	486	34	its	its	PRON
fnp-5883	486	35	structural	structural	ADJ
fnp-5883	486	36	integrity	integrity	NOUN
fnp-5883	486	37	.	.	PUNCT
fnp-5883	487	1	however	however	ADV
fnp-5883	487	2	,	,	PUNCT
fnp-5883	487	3	current	current	ADJ
fnp-5883	487	4	methods	method	NOUN
fnp-5883	487	5	struggle	struggle	VERB
fnp-5883	487	6	with	with	ADP
fnp-5883	487	7	cryopreserving	cryopreserve	VERB
fnp-5883	487	8	large	large	ADJ
fnp-5883	487	9	tissue	tissue	NOUN
fnp-5883	487	10	volumes	volume	NOUN
fnp-5883	487	11	without	without	ADP
fnp-5883	487	12	ice	ice	NOUN
fnp-5883	487	13	damage	damage	NOUN
fnp-5883	487	14	.	.	PUNCT
fnp-5883	488	1	future	future	ADJ
fnp-5883	488	2	studies	study	NOUN
fnp-5883	488	3	could	could	AUX
fnp-5883	488	4	explore	explore	VERB
fnp-5883	488	5	improved	improved	ADJ
fnp-5883	488	6	methods	method	NOUN
fnp-5883	488	7	for	for	ADP
fnp-5883	488	8	cryoprotectant	cryoprotectant	NOUN
fnp-5883	488	9	perfusion	perfusion	NOUN
fnp-5883	488	10	of	of	ADP
fnp-5883	488	11	the	the	DET
fnp-5883	488	12	brain	brain	NOUN
fnp-5883	488	13	,	,	PUNCT
fnp-5883	488	14	which	which	PRON
fnp-5883	488	15	has	have	AUX
fnp-5883	488	16	received	receive	VERB
fnp-5883	488	17	relatively	relatively	ADV
fnp-5883	488	18	little	little	ADJ
fnp-5883	488	19	attention	attention	NOUN
fnp-5883	488	20	in	in	ADP
fnp-5883	488	21	the	the	DET
fnp-5883	488	22	published	publish	VERB
fnp-5883	488	23	literature	literature	NOUN
fnp-5883	488	24	over	over	ADP
fnp-5883	488	25	the	the	DET
fnp-5883	488	26	years	year	NOUN
fnp-5883	488	27	(	(	PUNCT
fnp-5883	488	28	suda	suda	NOUN
fnp-5883	488	29	et	et	PROPN
fnp-5883	488	30	al	al	PROPN
fnp-5883	488	31	.	.	PROPN
fnp-5883	488	32	,	,	PUNCT
fnp-5883	488	33	1966	1966	NUM
fnp-5883	488	34	;	;	PUNCT
fnp-5883	488	35	lemler	lemler	PROPN
fnp-5883	488	36	et	et	PROPN
fnp-5883	488	37	al	al	PROPN
fnp-5883	488	38	.	.	PROPN
fnp-5883	488	39	,	,	PUNCT
fnp-5883	488	40	2004	2004	NUM
fnp-5883	488	41	;	;	PUNCT
fnp-5883	488	42	canatelli	canatelli	PROPN
fnp-5883	488	43	-	-	PUNCT
fnp-5883	488	44	mallat	mallat	PROPN
fnp-5883	488	45	et	et	PROPN
fnp-5883	488	46	al	al	PROPN
fnp-5883	488	47	.	.	PROPN
fnp-5883	488	48	,	,	PUNCT
fnp-5883	488	49	2020	2020	NUM
fnp-5883	488	50	;	;	PUNCT
fnp-5883	488	51	comba	comba	PROPN
fnp-5883	488	52	et	et	PROPN
fnp-5883	488	53	al	al	PROPN
fnp-5883	488	54	.	.	PROPN
fnp-5883	488	55	,	,	PUNCT
fnp-5883	488	56	2020	2020	NUM
fnp-5883	488	57	)	)	PUNCT
fnp-5883	488	58	.	.	PUNCT
fnp-5883	489	1	corroborating	corroborate	VERB
fnp-5883	489	2	and	and	CCONJ
fnp-5883	489	3	potentially	potentially	ADV
fnp-5883	489	4	improving	improve	VERB
fnp-5883	489	5	such	such	ADJ
fnp-5883	489	6	efforts	effort	NOUN
fnp-5883	489	7	could	could	AUX
fnp-5883	489	8	provide	provide	VERB
fnp-5883	489	9	insights	insight	NOUN
fnp-5883	489	10	into	into	ADP
fnp-5883	489	11	the	the	DET
fnp-5883	489	12	feasibility	feasibility	NOUN
fnp-5883	489	13	and	and	CCONJ
fnp-5883	489	14	the	the	DET
fnp-5883	489	15	limitations	limitation	NOUN
fnp-5883	489	16	of	of	ADP
fnp-5883	489	17	whole	whole	ADJ
fnp-5883	489	18	brain	brain	NOUN
fnp-5883	489	19	cryopreservation	cryopreservation	NOUN
fnp-5883	489	20	via	via	ADP
fnp-5883	489	21	cryoprotectant	cryoprotectant	NOUN
fnp-5883	489	22	perfusion	perfusion	NOUN
fnp-5883	489	23	.	.	PUNCT
fnp-5883	490	1	several	several	ADJ
fnp-5883	490	2	open	open	ADJ
fnp-5883	490	3	questions	question	NOUN
fnp-5883	490	4	also	also	ADV
fnp-5883	490	5	remain	remain	VERB
fnp-5883	490	6	regarding	regard	VERB
fnp-5883	490	7	the	the	DET
fnp-5883	490	8	basic	basic	ADJ
fnp-5883	490	9	mechanisms	mechanism	NOUN
fnp-5883	490	10	and	and	CCONJ
fnp-5883	490	11	limits	limit	NOUN
fnp-5883	490	12	of	of	ADP
fnp-5883	490	13	ice	ice	NOUN
fnp-5883	490	14	damage	damage	NOUN
fnp-5883	490	15	during	during	ADP
fnp-5883	490	16	cryopreservation	cryopreservation	NOUN
fnp-5883	490	17	.	.	PUNCT
fnp-5883	491	1	research	research	NOUN
fnp-5883	491	2	is	be	AUX
fnp-5883	491	3	needed	need	VERB
fnp-5883	491	4	to	to	PART
fnp-5883	491	5	better	well	ADV
fnp-5883	491	6	characterize	characterize	VERB
fnp-5883	491	7	the	the	DET
fnp-5883	491	8	ice	ice	NOUN
fnp-5883	491	9	damage	damage	NOUN
fnp-5883	491	10	caused	cause	VERB
fnp-5883	491	11	both	both	PRON
fnp-5883	491	12	during	during	ADP
fnp-5883	491	13	the	the	DET
fnp-5883	491	14	initial	initial	ADJ
fnp-5883	491	15	cooling	cooling	NOUN
fnp-5883	491	16	of	of	ADP
fnp-5883	491	17	brain	brain	NOUN
fnp-5883	491	18	tissue	tissue	NOUN
fnp-5883	491	19	and	and	CCONJ
fnp-5883	491	20	during	during	ADP
fnp-5883	491	21	the	the	DET
fnp-5883	491	22	rewarming	rewarming	NOUN
fnp-5883	491	23	of	of	ADP
fnp-5883	491	24	large	large	ADJ
fnp-5883	491	25	brain	brain	NOUN
fnp-5883	491	26	samples	sample	NOUN
fnp-5883	491	27	.	.	PUNCT
fnp-5883	492	1	technological	technological	ADJ
fnp-5883	492	2	approaches	approach	NOUN
fnp-5883	492	3	to	to	PART
fnp-5883	492	4	increase	increase	VERB
fnp-5883	492	5	the	the	DET
fnp-5883	492	6	speed	speed	NOUN
fnp-5883	492	7	of	of	ADP
fnp-5883	492	8	rewarming	rewarming	NOUN
fnp-5883	492	9	,	,	PUNCT
fnp-5883	492	10	such	such	ADJ
fnp-5883	492	11	as	as	ADP
fnp-5883	492	12	dielectric	dielectric	ADJ
fnp-5883	492	13	warming	warming	NOUN
fnp-5883	492	14	(	(	PUNCT
fnp-5883	492	15	wowk	wowk	PROPN
fnp-5883	492	16	et	et	PROPN
fnp-5883	492	17	al	al	PROPN
fnp-5883	492	18	.	.	PROPN
fnp-5883	492	19	,	,	PUNCT
fnp-5883	492	20	2024	2024	NUM
fnp-5883	492	21	)	)	PUNCT
fnp-5883	492	22	or	or	CCONJ
fnp-5883	492	23	ultrasound	ultrasound	NOUN
fnp-5883	492	24	rewarming	rewarming	NOUN
fnp-5883	492	25	(	(	PUNCT
fnp-5883	492	26	alcalá	alcalá	NOUN
fnp-5883	492	27	et	et	PROPN
fnp-5883	492	28	al	al	PROPN
fnp-5883	492	29	.	.	PROPN
fnp-5883	492	30	,	,	PUNCT
fnp-5883	492	31	2023	2023	NUM
fnp-5883	492	32	)	)	PUNCT
fnp-5883	492	33	,	,	PUNCT
fnp-5883	492	34	may	may	AUX
fnp-5883	492	35	have	have	VERB
fnp-5883	492	36	the	the	DET
fnp-5883	492	37	potential	potential	NOUN
fnp-5883	492	38	to	to	PART
fnp-5883	492	39	decrease	decrease	VERB
fnp-5883	492	40	damage	damage	NOUN
fnp-5883	492	41	in	in	ADP
fnp-5883	492	42	cryopreserved	cryopreserved	ADJ
fnp-5883	492	43	brain	brain	NOUN
fnp-5883	492	44	tissue	tissue	NOUN
fnp-5883	492	45	further	far	ADV
fnp-5883	492	46	,	,	PUNCT
fnp-5883	492	47	although	although	SCONJ
fnp-5883	492	48	this	this	PRON
fnp-5883	492	49	requires	require	VERB
fnp-5883	492	50	more	more	ADJ
fnp-5883	492	51	study	study	NOUN
fnp-5883	492	52	.	.	PUNCT
fnp-5883	493	1	finally	finally	ADV
fnp-5883	493	2	,	,	PUNCT
fnp-5883	493	3	the	the	DET
fnp-5883	493	4	use	use	NOUN
fnp-5883	493	5	of	of	ADP
fnp-5883	493	6	computational	computational	ADJ
fnp-5883	493	7	methods	method	NOUN
fnp-5883	493	8	to	to	PART
fnp-5883	493	9	infer	infer	VERB
fnp-5883	493	10	the	the	DET
fnp-5883	493	11	most	most	ADV
fnp-5883	493	12	probable	probable	ADJ
fnp-5883	493	13	original	original	ADJ
fnp-5883	493	14	state	state	NOUN
fnp-5883	493	15	of	of	ADP
fnp-5883	493	16	ice	ice	NOUN
fnp-5883	493	17	-	-	PUNCT
fnp-5883	493	18	damaged	damage	VERB
fnp-5883	493	19	tissue	tissue	NOUN
fnp-5883	493	20	is	be	AUX
fnp-5883	493	21	an	an	DET
fnp-5883	493	22	area	area	NOUN
fnp-5883	493	23	for	for	ADP
fnp-5883	493	24	future	future	ADJ
fnp-5883	493	25	work	work	NOUN
fnp-5883	493	26	.	.	PUNCT
fnp-5883	494	1	some	some	DET
fnp-5883	494	2	progress	progress	NOUN
fnp-5883	494	3	has	have	AUX
fnp-5883	494	4	already	already	ADV
fnp-5883	494	5	been	be	AUX
fnp-5883	494	6	made	make	VERB
fnp-5883	494	7	in	in	ADP
fnp-5883	494	8	this	this	DET
fnp-5883	494	9	direction	direction	NOUN
fnp-5883	494	10	for	for	ADP
fnp-5883	494	11	frozen	frozen	ADJ
fnp-5883	494	12	sections	section	NOUN
fnp-5883	494	13	(	(	PUNCT
fnp-5883	494	14	ozyoruk	ozyoruk	ADJ
fnp-5883	494	15	et	et	PROPN
fnp-5883	494	16	al	al	PROPN
fnp-5883	494	17	.	.	PROPN
fnp-5883	494	18	,	,	PUNCT
fnp-5883	494	19	2022	2022	NUM
fnp-5883	494	20	)	)	PUNCT
fnp-5883	494	21	.	.	PUNCT
fnp-5883	495	1	however	however	ADV
fnp-5883	495	2	,	,	PUNCT
fnp-5883	495	3	in	in	ADP
fnp-5883	495	4	this	this	DET
fnp-5883	495	5	study	study	NOUN
fnp-5883	495	6	,	,	PUNCT
fnp-5883	495	7	the	the	DET
fnp-5883	495	8	algorithm	algorithm	NOUN
fnp-5883	495	9	could	could	AUX
fnp-5883	495	10	not	not	PART
fnp-5883	495	11	reverse	reverse	VERB
fnp-5883	495	12	the	the	DET
fnp-5883	495	13	alterations	alteration	NOUN
fnp-5883	495	14	of	of	ADP
fnp-5883	495	15	cellular	cellular	ADJ
fnp-5883	495	16	positions	position	NOUN
fnp-5883	495	17	when	when	SCONJ
fnp-5883	495	18	severe	severe	ADJ
fnp-5883	495	19	ice	ice	NOUN
fnp-5883	495	20	artifacts	artifact	NOUN
fnp-5883	495	21	were	be	AUX
fnp-5883	495	22	present	present	ADJ
fnp-5883	495	23	.	.	PUNCT
fnp-5883	496	1	there	there	PRON
fnp-5883	496	2	is	be	VERB
fnp-5883	496	3	a	a	DET
fnp-5883	496	4	need	need	NOUN
fnp-5883	496	5	for	for	ADP
fnp-5883	496	6	a	a	DET
fnp-5883	496	7	better	well	ADJ
fnp-5883	496	8	theoretical	theoretical	ADJ
fnp-5883	496	9	understanding	understanding	NOUN
fnp-5883	496	10	of	of	ADP
fnp-5883	496	11	the	the	DET
fnp-5883	496	12	limits	limit	NOUN
fnp-5883	496	13	of	of	ADP
fnp-5883	496	14	such	such	ADJ
fnp-5883	496	15	computational	computational	ADJ
fnp-5883	496	16	inference	inference	NOUN
fnp-5883	496	17	methods	method	NOUN
fnp-5883	496	18	to	to	PART
fnp-5883	496	19	reverse	reverse	VERB
fnp-5883	496	20	ice	ice	NOUN
fnp-5883	496	21	damage	damage	NOUN
fnp-5883	496	22	.	.	PUNCT
fnp-5883	497	1	conclusions	conclusion	NOUN
fnp-5883	497	2	this	this	DET
fnp-5883	497	3	review	review	NOUN
fnp-5883	497	4	highlights	highlight	VERB
fnp-5883	497	5	the	the	DET
fnp-5883	497	6	complex	complex	ADJ
fnp-5883	497	7	landscape	landscape	NOUN
fnp-5883	497	8	of	of	ADP
fnp-5883	497	9	brain	brain	NOUN
fnp-5883	497	10	tissue	tissue	NOUN
fnp-5883	497	11	cryopreservation	cryopreservation	NOUN
fnp-5883	497	12	,	,	PUNCT
fnp-5883	497	13	revealing	reveal	VERB
fnp-5883	497	14	both	both	CCONJ
fnp-5883	497	15	the	the	DET
fnp-5883	497	16	potential	potential	NOUN
fnp-5883	497	17	and	and	CCONJ
fnp-5883	497	18	limitations	limitation	NOUN
fnp-5883	497	19	of	of	ADP
fnp-5883	497	20	current	current	ADJ
fnp-5883	497	21	methods	method	NOUN
fnp-5883	497	22	.	.	PUNCT
fnp-5883	498	1	key	key	ADJ
fnp-5883	498	2	themes	theme	NOUN
fnp-5883	498	3	that	that	PRON
fnp-5883	498	4	emerged	emerge	VERB
fnp-5883	498	5	from	from	ADP
fnp-5883	498	6	our	our	PRON
fnp-5883	498	7	literature	literature	NOUN
fnp-5883	498	8	review	review	NOUN
fnp-5883	498	9	included	include	VERB
fnp-5883	498	10	the	the	DET
fnp-5883	498	11	relationship	relationship	NOUN
fnp-5883	498	12	between	between	ADP
fnp-5883	498	13	cooling	cool	VERB
fnp-5883	498	14	rate	rate	NOUN
fnp-5883	498	15	and	and	CCONJ
fnp-5883	498	16	freezing	freezing	ADJ
fnp-5883	498	17	injury	injury	NOUN
fnp-5883	498	18	,	,	PUNCT
fnp-5883	498	19	the	the	DET
fnp-5883	498	20	effects	effect	NOUN
fnp-5883	498	21	of	of	ADP
fnp-5883	498	22	different	different	ADJ
fnp-5883	498	23	cryoprotectants	cryoprotectant	NOUN
fnp-5883	498	24	,	,	PUNCT
fnp-5883	498	25	and	and	CCONJ
fnp-5883	498	26	the	the	DET
fnp-5883	498	27	impact	impact	NOUN
fnp-5883	498	28	of	of	ADP
fnp-5883	498	29	tissue	tissue	NOUN
fnp-5883	498	30	characteristics	characteristic	NOUN
fnp-5883	498	31	on	on	ADP
fnp-5883	498	32	cryopreservation	cryopreservation	NOUN
fnp-5883	498	33	outcomes	outcome	NOUN
fnp-5883	498	34	.	.	PUNCT
fnp-5883	499	1	of	of	ADP
fnp-5883	499	2	the	the	DET
fnp-5883	499	3	study	study	NOUN
fnp-5883	499	4	types	type	NOUN
fnp-5883	499	5	identified	identify	VERB
fnp-5883	499	6	,	,	PUNCT
fnp-5883	499	7	cryofixation	cryofixation	NOUN
fnp-5883	499	8	offers	offer	VERB
fnp-5883	499	9	exceptional	exceptional	ADJ
fnp-5883	499	10	ultrastructural	ultrastructural	ADJ
fnp-5883	499	11	preservation	preservation	NOUN
fnp-5883	499	12	but	but	CCONJ
fnp-5883	499	13	is	be	AUX
fnp-5883	499	14	limited	limit	VERB
fnp-5883	499	15	to	to	ADP
fnp-5883	499	16	very	very	ADV
fnp-5883	499	17	thin	thin	ADJ
fnp-5883	499	18	samples	sample	NOUN
fnp-5883	499	19	,	,	PUNCT
fnp-5883	499	20	while	while	SCONJ
fnp-5883	499	21	freezing	freezing	ADJ
fnp-5883	499	22	approaches	approach	NOUN
fnp-5883	499	23	can	can	AUX
fnp-5883	499	24	achieve	achieve	VERB
fnp-5883	499	25	minimal	minimal	ADJ
fnp-5883	499	26	ice	ice	NOUN
fnp-5883	499	27	artifacts	artifact	NOUN
fnp-5883	499	28	as	as	SCONJ
fnp-5883	499	29	seen	see	VERB
fnp-5883	499	30	on	on	ADP
fnp-5883	499	31	light	light	ADJ
fnp-5883	499	32	microscopy	microscopy	NOUN
fnp-5883	499	33	in	in	ADP
fnp-5883	499	34	relatively	relatively	ADV
fnp-5883	499	35	larger	large	ADJ
fnp-5883	499	36	specimens	specimen	NOUN
fnp-5883	499	37	,	,	PUNCT
fnp-5883	499	38	particularly	particularly	ADV
fnp-5883	499	39	when	when	SCONJ
fnp-5883	499	40	rapid	rapid	ADJ
fnp-5883	499	41	cooling	cool	VERB
fnp-5883	499	42	methods	method	NOUN
fnp-5883	499	43	are	be	AUX
fnp-5883	499	44	employed	employ	VERB
fnp-5883	499	45	.	.	PUNCT
fnp-5883	500	1	the	the	DET
fnp-5883	500	2	use	use	NOUN
fnp-5883	500	3	of	of	ADP
fnp-5883	500	4	cryoprotectants	cryoprotectant	NOUN
fnp-5883	500	5	shows	show	VERB
fnp-5883	500	6	promise	promise	NOUN
fnp-5883	500	7	for	for	ADP
fnp-5883	500	8	high	high	ADJ
fnp-5883	500	9	-	-	PUNCT
fnp-5883	500	10	quality	quality	NOUN
fnp-5883	500	11	preservation	preservation	NOUN
fnp-5883	500	12	,	,	PUNCT
fnp-5883	500	13	but	but	CCONJ
fnp-5883	500	14	remains	remains	AUX
fnp-5883	500	15	challenging	challenge	VERB
fnp-5883	500	16	for	for	ADP
fnp-5883	500	17	achieving	achieve	VERB
fnp-5883	500	18	consistently	consistently	ADV
fnp-5883	500	19	even	even	ADV
fnp-5883	500	20	distribution	distribution	NOUN
fnp-5883	500	21	,	,	PUNCT
fnp-5883	500	22	especially	especially	ADV
fnp-5883	500	23	in	in	ADP
fnp-5883	500	24	larger	large	ADJ
fnp-5883	500	25	samples	sample	NOUN
fnp-5883	500	26	and	and	CCONJ
fnp-5883	500	27	postmortem	postmortem	NOUN
fnp-5883	500	28	settings	setting	NOUN
fnp-5883	500	29	.	.	PUNCT
fnp-5883	501	1	notably	notably	ADV
fnp-5883	501	2	,	,	PUNCT
fnp-5883	501	3	brain	brain	NOUN
fnp-5883	501	4	tissue	tissue	NOUN
fnp-5883	501	5	appears	appear	VERB
fnp-5883	501	6	to	to	PART
fnp-5883	501	7	be	be	AUX
fnp-5883	501	8	particularly	particularly	ADV
fnp-5883	501	9	vulnerable	vulnerable	ADJ
fnp-5883	501	10	to	to	ADP
fnp-5883	501	11	freezing	freeze	VERB
fnp-5883	501	12	damage	damage	NOUN
fnp-5883	501	13	as	as	ADP
fnp-5883	501	14	compared	compare	VERB
fnp-5883	501	15	to	to	ADP
fnp-5883	501	16	other	other	ADJ
fnp-5883	501	17	tissues	tissue	NOUN
fnp-5883	501	18	,	,	PUNCT
fnp-5883	501	19	underscoring	underscore	VERB
fnp-5883	501	20	the	the	DET
fnp-5883	501	21	need	need	NOUN
fnp-5883	501	22	for	for	ADP
fnp-5883	501	23	more	more	ADJ
fnp-5883	501	24	research	research	NOUN
fnp-5883	501	25	in	in	ADP
fnp-5883	501	26	this	this	DET
fnp-5883	501	27	area	area	NOUN
fnp-5883	501	28	.	.	PUNCT
fnp-5883	502	1	while	while	SCONJ
fnp-5883	502	2	current	current	ADJ
fnp-5883	502	3	cryopreservation	cryopreservation	NOUN
fnp-5883	502	4	techniques	technique	NOUN
fnp-5883	502	5	are	be	AUX
fnp-5883	502	6	sufficient	sufficient	ADJ
fnp-5883	502	7	for	for	ADP
fnp-5883	502	8	many	many	ADJ
fnp-5883	502	9	research	research	NOUN
fnp-5883	502	10	applications	application	NOUN
fnp-5883	502	11	,	,	PUNCT
fnp-5883	502	12	there	there	PRON
fnp-5883	502	13	remains	remain	VERB
fnp-5883	502	14	an	an	DET
fnp-5883	502	15	unmet	unmet	NOUN
fnp-5883	502	16	need	need	NOUN
fnp-5883	502	17	for	for	ADP
fnp-5883	502	18	methods	method	NOUN
fnp-5883	502	19	capable	capable	ADJ
fnp-5883	502	20	of	of	ADP
fnp-5883	502	21	preserving	preserve	VERB
fnp-5883	502	22	larger	large	ADJ
fnp-5883	502	23	brain	brain	NOUN
fnp-5883	502	24	samples	sample	NOUN
fnp-5883	502	25	with	with	ADP
fnp-5883	502	26	minimal	minimal	ADJ
fnp-5883	502	27	structural	structural	ADJ
fnp-5883	502	28	disruption	disruption	NOUN
fnp-5883	502	29	.	.	PUNCT
fnp-5883	503	1	future	future	ADJ
fnp-5883	503	2	research	research	NOUN
fnp-5883	503	3	may	may	AUX
fnp-5883	503	4	benefit	benefit	VERB
fnp-5883	503	5	from	from	ADP
fnp-5883	503	6	a	a	DET
fnp-5883	503	7	focus	focus	NOUN
fnp-5883	503	8	on	on	ADP
fnp-5883	503	9	improving	improve	VERB
fnp-5883	503	10	cryoprotectant	cryoprotectant	NOUN
fnp-5883	503	11	delivery	delivery	NOUN
fnp-5883	503	12	,	,	PUNCT
fnp-5883	503	13	optimizing	optimize	VERB
fnp-5883	503	14	cooling	cool	VERB
fnp-5883	503	15	and	and	CCONJ
fnp-5883	503	16	rewarming	rewarme	VERB
fnp-5883	503	17	protocols	protocol	NOUN
fnp-5883	503	18	,	,	PUNCT
fnp-5883	503	19	and	and	CCONJ
fnp-5883	503	20	exploring	explore	VERB
fnp-5883	503	21	computational	computational	ADJ
fnp-5883	503	22	methods	method	NOUN
fnp-5883	503	23	to	to	PART
fnp-5883	503	24	infer	infer	VERB
fnp-5883	503	25	the	the	DET
fnp-5883	503	26	original	original	ADJ
fnp-5883	503	27	state	state	NOUN
fnp-5883	503	28	of	of	ADP
fnp-5883	503	29	the	the	DET
fnp-5883	503	30	tissue	tissue	NOUN
fnp-5883	503	31	despite	despite	SCONJ
fnp-5883	503	32	the	the	DET
fnp-5883	503	33	presence	presence	NOUN
fnp-5883	503	34	of	of	ADP
fnp-5883	503	35	freezing	freeze	VERB
fnp-5883	503	36	artifacts	artifact	NOUN
fnp-5883	503	37	.	.	PUNCT
fnp-5883	504	1	abbreviations	abbreviation	NOUN
fnp-5883	504	2	dmso	dmso	NOUN
fnp-5883	504	3	dimethyl	dimethyl	NOUN
fnp-5883	504	4	sulfoxide	sulfoxide	NOUN
fnp-5883	504	5	;	;	PUNCT
fnp-5883	504	6	oct	oct	PROPN
fnp-5883	504	7	optimal	optimal	ADJ
fnp-5883	504	8	cutting	cut	VERB
fnp-5883	504	9	temperature	temperature	NOUN
fnp-5883	504	10	compound	compound	NOUN
fnp-5883	504	11	;	;	PUNCT
fnp-5883	504	12	pmi	pmi	NOUN
fnp-5883	504	13	postmortem	postmortem	NOUN
fnp-5883	504	14	interval	interval	NOUN
fnp-5883	504	15	;	;	PUNCT
fnp-5883	504	16	tem	tem	PROPN
fnp-5883	504	17	transmission	transmission	NOUN
fnp-5883	504	18	electron	electron	NOUN
fnp-5883	504	19	microscopy	microscopy	NOUN
fnp-5883	504	20	;	;	PUNCT
fnp-5883	504	21	vm1	vm1	NOUN
fnp-5883	504	22	and	and	CCONJ
fnp-5883	504	23	vm3	vm3	ADP
fnp-5883	504	24	vitrification	vitrification	NOUN
fnp-5883	504	25	solutions	solution	NOUN
fnp-5883	504	26	composed	compose	VERB
fnp-5883	504	27	of	of	ADP
fnp-5883	504	28	multiple	multiple	ADJ
fnp-5883	504	29	cryoprotectants	cryoprotectant	NOUN
fnp-5883	504	30	.	.	PUNCT
fnp-5883	505	1	acknowledgements	acknowledgement	NOUN
fnp-5883	505	2	we	we	PRON
fnp-5883	505	3	would	would	AUX
fnp-5883	505	4	like	like	VERB
fnp-5883	505	5	to	to	PART
fnp-5883	505	6	thank	thank	VERB
fnp-5883	505	7	brian	brian	PROPN
fnp-5883	505	8	wowk	wowk	PROPN
fnp-5883	505	9	and	and	CCONJ
fnp-5883	505	10	kenneth	kenneth	PROPN
fnp-5883	505	11	hayworth	hayworth	PROPN
fnp-5883	505	12	for	for	ADP
fnp-5883	505	13	helpful	helpful	ADJ
fnp-5883	505	14	personal	personal	ADJ
fnp-5883	505	15	communications	communication	NOUN
fnp-5883	505	16	regarding	regard	VERB
fnp-5883	505	17	this	this	DET
fnp-5883	505	18	topic	topic	NOUN
fnp-5883	505	19	.	.	PUNCT
fnp-5883	506	1	data	datum	NOUN
fnp-5883	506	2	availability	availability	NOUN
fnp-5883	506	3	this	this	DET
fnp-5883	506	4	article	article	NOUN
fnp-5883	506	5	was	be	AUX
fnp-5883	506	6	primarily	primarily	ADV
fnp-5883	506	7	built	build	VERB
fnp-5883	506	8	upon	upon	SCONJ
fnp-5883	506	9	publicly	publicly	ADV
fnp-5883	506	10	available	available	ADJ
fnp-5883	506	11	data	datum	NOUN
fnp-5883	506	12	.	.	PUNCT
fnp-5883	507	1	the	the	DET
fnp-5883	507	2	relevant	relevant	ADJ
fnp-5883	507	3	data	datum	NOUN
fnp-5883	507	4	and	and	CCONJ
fnp-5883	507	5	analyses	analysis	NOUN
fnp-5883	507	6	are	be	AUX
fnp-5883	507	7	presented	present	VERB
fnp-5883	507	8	in	in	ADP
fnp-5883	507	9	the	the	DET
fnp-5883	507	10	article	article	NOUN
fnp-5883	507	11	or	or	CCONJ
fnp-5883	507	12	in	in	ADP
fnp-5883	507	13	the	the	DET
fnp-5883	507	14	supplementary	supplementary	ADJ
fnp-5883	507	15	materials	material	NOUN
fnp-5883	507	16	.	.	PUNCT
fnp-5883	508	1	author	author	NOUN
fnp-5883	508	2	contributions	contribution	NOUN
fnp-5883	508	3	a.t.m	a.t.m	PROPN
fnp-5883	508	4	.	.	PROPN
fnp-5883	508	5	,	,	PUNCT
fnp-5883	508	6	e.f.k	e.f.k	VERB
fnp-5883	508	7	.	.	PROPN
fnp-5883	508	8	,	,	PUNCT
fnp-5883	508	9	k.f	k.f	PROPN
fnp-5883	508	10	.	.	PROPN
fnp-5883	508	11	,	,	PUNCT
fnp-5883	508	12	and	and	CCONJ
fnp-5883	508	13	j.c	j.c	PROPN
fnp-5883	508	14	.	.	PROPN
fnp-5883	508	15	conceptualized	conceptualize	VERB
fnp-5883	508	16	the	the	DET
fnp-5883	508	17	article	article	NOUN
fnp-5883	508	18	.	.	PUNCT
fnp-5883	509	1	a.t.m	a.t.m	PROPN
fnp-5883	509	2	.	.	PROPN
fnp-5883	509	3	performed	perform	VERB
fnp-5883	509	4	article	article	NOUN
fnp-5883	509	5	searching	search	VERB
fnp-5883	509	6	and	and	CCONJ
fnp-5883	509	7	screening	screen	VERB
fnp-5883	509	8	.	.	PUNCT
fnp-5883	510	1	a.t.m	a.t.m	PROPN
fnp-5883	510	2	.	.	PROPN
fnp-5883	510	3	,	,	PUNCT
fnp-5883	510	4	o.n	o.n	PROPN
fnp-5883	510	5	.	.	PROPN
fnp-5883	510	6	,	,	PUNCT
fnp-5883	510	7	and	and	CCONJ
fnp-5883	510	8	b.w	b.w	PROPN
fnp-5883	510	9	.	.	PROPN
fnp-5883	510	10	performed	perform	VERB
fnp-5883	510	11	data	data	NOUN
fnp-5883	510	12	extraction	extraction	NOUN
fnp-5883	510	13	and	and	CCONJ
fnp-5883	510	14	review	review	NOUN
fnp-5883	510	15	of	of	ADP
fnp-5883	510	16	data	datum	NOUN
fnp-5883	510	17	extraction	extraction	NOUN
fnp-5883	510	18	.	.	PUNCT
fnp-5883	511	1	e.t	e.t	PROPN
fnp-5883	511	2	.	.	PROPN
fnp-5883	511	3	performed	perform	VERB
fnp-5883	511	4	experimental	experimental	ADJ
fnp-5883	511	5	studies	study	NOUN
fnp-5883	511	6	and	and	CCONJ
fnp-5883	511	7	imaging	imaging	NOUN
fnp-5883	511	8	of	of	ADP
fnp-5883	511	9	frozen	frozen	ADJ
fnp-5883	511	10	-	-	PUNCT
fnp-5883	511	11	thawed	thaw	VERB
fnp-5883	511	12	brain	brain	NOUN
fnp-5883	511	13	tissue	tissue	NOUN
fnp-5883	511	14	.	.	PUNCT
fnp-5883	512	1	a.t.m	a.t.m	PROPN
fnp-5883	512	2	.	.	PROPN
fnp-5883	512	3	wrote	write	VERB
fnp-5883	512	4	the	the	DET
fnp-5883	512	5	initial	initial	ADJ
fnp-5883	512	6	draft	draft	NOUN
fnp-5883	512	7	of	of	ADP
fnp-5883	512	8	the	the	DET
fnp-5883	512	9	manuscript	manuscript	NOUN
fnp-5883	512	10	.	.	PUNCT
fnp-5883	513	1	all	all	DET
fnp-5883	513	2	authors	author	NOUN
fnp-5883	513	3	reviewed	review	VERB
fnp-5883	513	4	the	the	DET
fnp-5883	513	5	manuscript	manuscript	NOUN
fnp-5883	513	6	.	.	PUNCT
fnp-5883	514	1	all	all	DET
fnp-5883	514	2	authors	author	NOUN
fnp-5883	514	3	approved	approve	VERB
fnp-5883	514	4	the	the	DET
fnp-5883	514	5	final	final	ADJ
fnp-5883	514	6	manuscript	manuscript	NOUN
fnp-5883	514	7	.	.	PUNCT
fnp-5883	515	1	conflicts	conflict	NOUN
fnp-5883	515	2	of	of	ADP
fnp-5883	515	3	interest	interest	NOUN
fnp-5883	515	4	statement	statement	NOUN
fnp-5883	515	5	a.t.m	a.t.m	PROPN
fnp-5883	515	6	.	.	PROPN
fnp-5883	515	7	is	be	AUX
fnp-5883	515	8	an	an	DET
fnp-5883	515	9	employee	employee	NOUN
fnp-5883	515	10	of	of	ADP
fnp-5883	515	11	oregon	oregon	PROPN
fnp-5883	515	12	brain	brain	PROPN
fnp-5883	515	13	preservation	preservation	NOUN
fnp-5883	515	14	,	,	PUNCT
fnp-5883	515	15	a	a	DET
fnp-5883	515	16	non	non	ADJ
fnp-5883	515	17	-	-	ADJ
fnp-5883	515	18	profit	profit	ADJ
fnp-5883	515	19	brain	brain	NOUN
fnp-5883	515	20	preservation	preservation	NOUN
fnp-5883	515	21	organization	organization	NOUN
fnp-5883	515	22	,	,	PUNCT
fnp-5883	515	23	and	and	CCONJ
fnp-5883	515	24	a	a	DET
fnp-5883	515	25	director	director	NOUN
fnp-5883	515	26	of	of	ADP
fnp-5883	515	27	apex	apex	PROPN
fnp-5883	515	28	neuroscience	neuroscience	NOUN
fnp-5883	515	29	,	,	PUNCT
fnp-5883	515	30	a	a	DET
fnp-5883	515	31	non	non	ADJ
fnp-5883	515	32	-	-	ADJ
fnp-5883	515	33	profit	profit	ADJ
fnp-5883	515	34	research	research	NOUN
fnp-5883	515	35	organization	organization	NOUN
fnp-5883	515	36	.	.	PUNCT
fnp-5883	516	1	b.w	b.w	PROPN
fnp-5883	516	2	.	.	PROPN
fnp-5883	516	3	is	be	AUX
fnp-5883	516	4	affiliated	affiliate	VERB
fnp-5883	516	5	with	with	ADP
fnp-5883	516	6	the	the	DET
fnp-5883	516	7	european	european	PROPN
fnp-5883	516	8	institute	institute	PROPN
fnp-5883	516	9	for	for	ADP
fnp-5883	516	10	brain	brain	NOUN
fnp-5883	516	11	research	research	NOUN
fnp-5883	516	12	,	,	PUNCT
fnp-5883	516	13	a	a	DET
fnp-5883	516	14	non	non	ADJ
fnp-5883	516	15	-	-	ADJ
fnp-5883	516	16	profit	profit	ADJ
fnp-5883	516	17	research	research	NOUN
fnp-5883	516	18	foundation	foundation	NOUN
fnp-5883	516	19	,	,	PUNCT
fnp-5883	516	20	and	and	CCONJ
fnp-5883	516	21	the	the	DET
fnp-5883	516	22	biopreservation	biopreservation	NOUN
fnp-5883	516	23	institute	institute	PROPN
fnp-5883	516	24	,	,	PUNCT
fnp-5883	516	25	a	a	DET
fnp-5883	516	26	non	non	ADJ
fnp-5883	516	27	-	-	ADJ
fnp-5883	516	28	profit	profit	ADJ
fnp-5883	516	29	research	research	NOUN
fnp-5883	516	30	organization	organization	NOUN
fnp-5883	516	31	that	that	PRON
fnp-5883	516	32	works	work	VERB
fnp-5883	516	33	together	together	ADV
fnp-5883	516	34	with	with	ADP
fnp-5883	516	35	nectome	nectome	PROPN
fnp-5883	516	36	inc	inc	PROPN
fnp-5883	516	37	.	.	PROPN
fnp-5883	516	38	,	,	PUNCT
fnp-5883	516	39	which	which	PRON
fnp-5883	516	40	plans	plan	VERB
fnp-5883	516	41	to	to	PART
fnp-5883	516	42	offer	offer	VERB
fnp-5883	516	43	aldehyde	aldehyde	NOUN
fnp-5883	516	44	-	-	PUNCT
fnp-5883	516	45	based	base	VERB
fnp-5883	516	46	biostasis	biostasis	NOUN
fnp-5883	516	47	.	.	PUNCT
fnp-5883	517	1	e.f.k	e.f.k	NOUN
fnp-5883	517	2	.	.	PUNCT
fnp-5883	518	1	is	be	AUX
fnp-5883	518	2	a	a	DET
fnp-5883	518	3	shareholder	shareholder	NOUN
fnp-5883	518	4	and	and	CCONJ
fnp-5883	518	5	ceo	ceo	NOUN
fnp-5883	518	6	of	of	ADP
fnp-5883	518	7	tomorrow	tomorrow	NOUN
fnp-5883	518	8	biostasis	biostasis	NOUN
fnp-5883	518	9	,	,	PUNCT
fnp-5883	518	10	a	a	DET
fnp-5883	518	11	biostasis	biostasis	NOUN
fnp-5883	518	12	provider	provider	NOUN
fnp-5883	518	13	,	,	PUNCT
fnp-5883	518	14	and	and	CCONJ
fnp-5883	518	15	president	president	NOUN
fnp-5883	518	16	of	of	ADP
fnp-5883	518	17	the	the	DET
fnp-5883	518	18	board	board	NOUN
fnp-5883	518	19	of	of	ADP
fnp-5883	518	20	the	the	DET
fnp-5883	518	21	european	european	PROPN
fnp-5883	518	22	biostasis	biostasis	PROPN
fnp-5883	518	23	foundation	foundation	PROPN
fnp-5883	518	24	,	,	PUNCT
fnp-5883	518	25	a	a	DET
fnp-5883	518	26	non	non	ADJ
fnp-5883	518	27	-	-	ADJ
fnp-5883	518	28	profit	profit	ADJ
fnp-5883	518	29	research	research	NOUN
fnp-5883	518	30	foundation	foundation	PROPN
fnp-5883	518	31	.	.	PUNCT
fnp-5883	519	1	funding	funding	NOUN
fnp-5883	519	2	statement	statement	NOUN
fnp-5883	519	3	borys	borys	PROPN
fnp-5883	519	4	wróbel	wróbel	NOUN
fnp-5883	519	5	’s	’s	PART
fnp-5883	519	6	research	research	NOUN
fnp-5883	519	7	is	be	AUX
fnp-5883	519	8	supported	support	VERB
fnp-5883	519	9	by	by	ADP
fnp-5883	519	10	a	a	DET
fnp-5883	519	11	grant	grant	NOUN
fnp-5883	519	12	from	from	ADP
fnp-5883	519	13	the	the	DET
fnp-5883	519	14	biomedical	biomedical	ADJ
fnp-5883	519	15	research	research	NOUN
fnp-5883	519	16	and	and	CCONJ
fnp-5883	519	17	longevity	longevity	NOUN
fnp-5883	519	18	society	society	NOUN
fnp-5883	519	19	.	.	PUNCT
fnp-5883	520	1	otherwise	otherwise	ADV
fnp-5883	520	2	,	,	PUNCT
fnp-5883	520	3	the	the	DET
fnp-5883	520	4	authors	author	NOUN
fnp-5883	520	5	received	receive	VERB
fnp-5883	520	6	no	no	DET
fnp-5883	520	7	specific	specific	ADJ
fnp-5883	520	8	funding	funding	NOUN
fnp-5883	520	9	for	for	ADP
fnp-5883	520	10	this	this	DET
fnp-5883	520	11	work	work	NOUN
fnp-5883	520	12	.	.	PUNCT
fnp-5883	521	1	supplementary	supplementary	ADJ
fnp-5883	521	2	files	file	NOUN
fnp-5883	521	3	supplementary	supplementary	ADJ
fnp-5883	521	4	file	file	NOUN
fnp-5883	521	5	 	 	SPACE
fnp-5883	521	6	1	1	NUM
fnp-5883	521	7	[	[	NOUN
fnp-5883	521	8	download	download	NOUN
fnp-5883	521	9	]	]	PUNCT
fnp-5883	521	10	rameses	ramese	VERB
fnp-5883	521	11	checklist	checklist	NOUN
fnp-5883	521	12	[	[	X
fnp-5883	521	13	pdf	pdf	NOUN
fnp-5883	521	14	format	format	NOUN
fnp-5883	521	15	]	]	PUNCT
fnp-5883	521	16	.	.	PUNCT
fnp-5883	522	1	supplementary	supplementary	ADJ
fnp-5883	522	2	file	file	NOUN
fnp-5883	522	3	 	 	SPACE
fnp-5883	522	4	2	2	NUM
fnp-5883	522	5	[	[	NOUN
fnp-5883	522	6	download	download	NOUN
fnp-5883	522	7	]	]	X
fnp-5883	522	8	abstract	abstract	ADJ
fnp-5883	522	9	screening	screening	NOUN
fnp-5883	522	10	results	result	NOUN
fnp-5883	522	11	[	[	PUNCT
fnp-5883	522	12	csv	csv	NOUN
fnp-5883	522	13	format	format	NOUN
fnp-5883	522	14	]	]	PUNCT
fnp-5883	522	15	.	.	PUNCT
fnp-5883	523	1	supplementary	supplementary	ADJ
fnp-5883	523	2	file	file	NOUN
fnp-5883	523	3	 	 	SPACE
fnp-5883	523	4	3	3	NUM
fnp-5883	523	5	[	[	X
fnp-5883	523	6	download	download	NOUN
fnp-5883	523	7	]	]	X
fnp-5883	523	8	full	full	ADJ
fnp-5883	523	9	text	text	NOUN
fnp-5883	523	10	screening	screening	NOUN
fnp-5883	523	11	results	result	NOUN
fnp-5883	523	12	[	[	PUNCT
fnp-5883	523	13	csv	csv	NOUN
fnp-5883	523	14	format	format	NOUN
fnp-5883	523	15	]	]	PUNCT
fnp-5883	523	16	.	.	PUNCT
fnp-5883	524	1	supplementary	supplementary	ADJ
fnp-5883	524	2	file	file	NOUN
fnp-5883	524	3	 	 	SPACE
fnp-5883	524	4	4	4	NUM
fnp-5883	525	1	[	[	NOUN
fnp-5883	525	2	download	download	NOUN
fnp-5883	525	3	]	]	PUNCT
fnp-5883	525	4	extracted	extract	VERB
fnp-5883	525	5	data	data	NOUN
fnp-5883	525	6	table	table	NOUN
fnp-5883	525	7	[	[	X
fnp-5883	525	8	xlsx	xlsx	NOUN
fnp-5883	525	9	format	format	NOUN
fnp-5883	525	10	]	]	PUNCT
fnp-5883	525	11	.	.	PUNCT
fnp-5883	526	1	references	reference	NOUN
fnp-5883	526	2	adam	adam	PROPN
fnp-5883	526	3	,	,	PUNCT
fnp-5883	526	4	m.	m.	NOUN
fnp-5883	526	5	,	,	PUNCT
fnp-5883	526	6	hu	hu	PROPN
fnp-5883	526	7	,	,	PUNCT
fnp-5883	526	8	j.f	j.f	PROPN
fnp-5883	526	9	.	.	PROPN
fnp-5883	526	10	,	,	PUNCT
fnp-5883	526	11	lange	lange	PROPN
fnp-5883	526	12	,	,	PUNCT
fnp-5883	526	13	p.	p.	NOUN
fnp-5883	526	14	,	,	PUNCT
fnp-5883	526	15	wolfinbarger	wolfinbarger	NOUN
fnp-5883	526	16	,	,	PUNCT
fnp-5883	526	17	l.	l.	PROPN
fnp-5883	526	18	,	,	PUNCT
fnp-5883	526	19	1990	1990	NUM
fnp-5883	526	20	.	.	PUNCT
fnp-5883	527	1	the	the	DET
fnp-5883	527	2	effect	effect	NOUN
fnp-5883	527	3	of	of	ADP
fnp-5883	527	4	liquid	liquid	ADJ
fnp-5883	527	5	nitrogen	nitrogen	NOUN
fnp-5883	527	6	submersion	submersion	NOUN
fnp-5883	527	7	on	on	ADP
fnp-5883	527	8	cryopreserved	cryopreserved	ADJ
fnp-5883	527	9	human	human	ADJ
fnp-5883	527	10	heart	heart	NOUN
fnp-5883	527	11	valves	valve	NOUN
fnp-5883	527	12	.	.	PUNCT
fnp-5883	528	1	cryobiology	cryobiology	NOUN
fnp-5883	528	2	27	27	NUM
fnp-5883	528	3	,	,	PUNCT
fnp-5883	528	4	605–614	605–614	NUM
fnp-5883	528	5	.	.	PUNCT
fnp-5883	529	1	https://doi.org/10.1016/s0011-2240(05)80028-8	https://doi.org/10.1016/s0011-2240(05)80028-8	PROPN
fnp-5883	529	2	alcalá	alcalá	PROPN
fnp-5883	529	3	,	,	PUNCT
fnp-5883	529	4	e.	e.	PROPN
fnp-5883	529	5	,	,	PUNCT
fnp-5883	529	6	encabo	encabo	PROPN
fnp-5883	529	7	,	,	PUNCT
fnp-5883	529	8	l.	l.	PROPN
fnp-5883	529	9	,	,	PUNCT
fnp-5883	529	10	barroso	barroso	PROPN
fnp-5883	529	11	,	,	PUNCT
fnp-5883	529	12	f.	f.	PROPN
fnp-5883	529	13	,	,	PUNCT
fnp-5883	529	14	puentes	puentes	PROPN
fnp-5883	529	15	,	,	PUNCT
fnp-5883	529	16	a.	a.	PROPN
fnp-5883	529	17	,	,	PUNCT
fnp-5883	529	18	risco	risco	PROPN
fnp-5883	529	19	,	,	PUNCT
fnp-5883	529	20	i.	i.	PROPN
fnp-5883	529	21	,	,	PUNCT
fnp-5883	529	22	risco	risco	PROPN
fnp-5883	529	23	,	,	PUNCT
fnp-5883	529	24	r.	r.	PROPN
fnp-5883	529	25	,	,	PUNCT
fnp-5883	529	26	2023	2023	NUM
fnp-5883	529	27	.	.	PUNCT
fnp-5883	530	1	sound	sound	ADJ
fnp-5883	530	2	waves	wave	NOUN
fnp-5883	530	3	for	for	ADP
fnp-5883	530	4	solving	solve	VERB
fnp-5883	530	5	the	the	DET
fnp-5883	530	6	problem	problem	NOUN
fnp-5883	530	7	of	of	ADP
fnp-5883	530	8	recrystallization	recrystallization	NOUN
fnp-5883	530	9	in	in	ADP
fnp-5883	530	10	cryopreservation	cryopreservation	NOUN
fnp-5883	530	11	.	.	PUNCT
fnp-5883	531	1	sci	sci	PROPN
fnp-5883	531	2	.	.	PROPN
fnp-5883	531	3	rep	rep	PROPN
fnp-5883	531	4	.	.	PROPN
fnp-5883	531	5	13	13	NUM
fnp-5883	531	6	,	,	PUNCT
fnp-5883	531	7	7603	7603	NUM
fnp-5883	531	8	.	.	PUNCT
fnp-5883	532	1	https://doi.org/10.1038/s41598-023-34681-z	https://doi.org/10.1038/s41598-023-34681-z	NOUN
fnp-5883	532	2	andreasson	andreasson	NOUN
fnp-5883	532	3	,	,	PUNCT
fnp-5883	532	4	a.	a.	NOUN
fnp-5883	532	5	,	,	PUNCT
fnp-5883	532	6	kiss	kiss	PROPN
fnp-5883	532	7	,	,	PUNCT
fnp-5883	532	8	n.b	n.b	PROPN
fnp-5883	532	9	.	.	PROPN
fnp-5883	532	10	,	,	PUNCT
fnp-5883	532	11	juhlin	juhlin	PROPN
fnp-5883	532	12	,	,	PUNCT
fnp-5883	532	13	c.c	c.c	PROPN
fnp-5883	532	14	.	.	PROPN
fnp-5883	532	15	,	,	PUNCT
fnp-5883	532	16	höög	höög	PROPN
fnp-5883	532	17	,	,	PUNCT
fnp-5883	532	18	a.	a.	NOUN
fnp-5883	532	19	,	,	PUNCT
fnp-5883	532	20	2013	2013	NUM
fnp-5883	532	21	.	.	PUNCT
fnp-5883	533	1	long	long	ADJ
fnp-5883	533	2	-	-	PUNCT
fnp-5883	533	3	term	term	NOUN
fnp-5883	533	4	storage	storage	NOUN
fnp-5883	533	5	of	of	ADP
fnp-5883	533	6	endocrine	endocrine	ADJ
fnp-5883	533	7	tissues	tissue	NOUN
fnp-5883	533	8	at	at	ADP
fnp-5883	533	9	-80	-80	NOUN
fnp-5883	533	10	 	 	SPACE
fnp-5883	533	11	°	°	NOUN
fnp-5883	533	12	c	c	NOUN
fnp-5883	533	13	does	do	AUX
fnp-5883	533	14	not	not	PART
fnp-5883	533	15	adversely	adversely	ADV
fnp-5883	533	16	affect	affect	VERB
fnp-5883	533	17	rna	rna	NOUN
fnp-5883	533	18	quality	quality	NOUN
fnp-5883	533	19	or	or	CCONJ
fnp-5883	533	20	overall	overall	ADJ
fnp-5883	533	21	histomorphology	histomorphology	NOUN
fnp-5883	533	22	.	.	PUNCT
fnp-5883	534	1	biopreservation	biopreservation	NOUN
fnp-5883	534	2	biobanking	biobanke	VERB
fnp-5883	534	3	11	11	NUM
fnp-5883	534	4	,	,	PUNCT
fnp-5883	534	5	366–370	366–370	NUM
fnp-5883	534	6	.	.	PUNCT
fnp-5883	535	1	https://doi.org/10.1089/bio.2013.0038	https://doi.org/10.1089/bio.2013.0038	PROPN
fnp-5883	535	2	annese	annese	PROPN
fnp-5883	535	3	,	,	PUNCT
fnp-5883	535	4	j.	j.	PROPN
fnp-5883	535	5	,	,	PUNCT
fnp-5883	535	6	2004	2004	NUM
fnp-5883	535	7	.	.	PUNCT
fnp-5883	536	1	neo	neo	NOUN
fnp-5883	536	2	-	-	NOUN
fnp-5883	536	3	architectonics	architectonic	NOUN
fnp-5883	536	4	:	:	PUNCT
fnp-5883	536	5	computer	computer	NOUN
fnp-5883	536	6	aided	aid	VERB
fnp-5883	536	7	histological	histological	ADJ
fnp-5883	536	8	examination	examination	NOUN
fnp-5883	536	9	of	of	ADP
fnp-5883	536	10	the	the	DET
fnp-5883	536	11	human	human	ADJ
fnp-5883	536	12	visual	visual	ADJ
fnp-5883	536	13	cortex	cortex	NOUN
fnp-5883	536	14	(	(	PUNCT
fnp-5883	536	15	ph.d	ph.d	PROPN
fnp-5883	536	16	.	.	PUNCT
fnp-5883	536	17	)	)	PUNCT
fnp-5883	536	18	.	.	PUNCT
fnp-5883	537	1	dartmouth	dartmouth	PROPN
fnp-5883	537	2	college	college	PROPN
fnp-5883	537	3	,	,	PUNCT
fnp-5883	537	4	united	united	PROPN
fnp-5883	537	5	states	states	PROPN
fnp-5883	537	6	-new	-new	PROPN
fnp-5883	537	7	hampshire	hampshire	PROPN
fnp-5883	537	8	.	.	PUNCT
fnp-5883	538	1	ansari	ansari	PROPN
fnp-5883	538	2	,	,	PUNCT
fnp-5883	538	3	k.a	k.a	PROPN
fnp-5883	538	4	.	.	PROPN
fnp-5883	538	5	,	,	PUNCT
fnp-5883	538	6	rand	rand	NOUN
fnp-5883	538	7	,	,	PUNCT
fnp-5883	538	8	a.	a.	PROPN
fnp-5883	538	9	,	,	PUNCT
fnp-5883	538	10	hendrickson	hendrickson	PROPN
fnp-5883	538	11	,	,	PUNCT
fnp-5883	538	12	h.	h.	PROPN
fnp-5883	538	13	,	,	PUNCT
fnp-5883	538	14	bentley	bentley	PROPN
fnp-5883	538	15	,	,	PUNCT
fnp-5883	538	16	m.d	m.d	PROPN
fnp-5883	538	17	.	.	PROPN
fnp-5883	538	18	,	,	PUNCT
fnp-5883	538	19	1976	1976	NUM
fnp-5883	538	20	.	.	PUNCT
fnp-5883	539	1	qualitative	qualitative	ADJ
fnp-5883	539	2	and	and	CCONJ
fnp-5883	539	3	quantitative	quantitative	ADJ
fnp-5883	539	4	studies	study	NOUN
fnp-5883	539	5	on	on	ADP
fnp-5883	539	6	human	human	ADJ
fnp-5883	539	7	myelin	myelin	NOUN
fnp-5883	539	8	basic	basic	ADJ
fnp-5883	539	9	protein	protein	NOUN
fnp-5883	539	10	in	in	ADP
fnp-5883	539	11	situ	situ	NOUN
fnp-5883	539	12	with	with	ADP
fnp-5883	539	13	respect	respect	NOUN
fnp-5883	539	14	to	to	ADP
fnp-5883	539	15	time	time	NOUN
fnp-5883	539	16	interval	interval	NOUN
fnp-5883	539	17	between	between	ADP
fnp-5883	539	18	death	death	NOUN
fnp-5883	539	19	and	and	CCONJ
fnp-5883	539	20	autopsy	autopsy	ADJ
fnp-5883	539	21	.	.	PUNCT
fnp-5883	540	1	j.	j.	PROPN
fnp-5883	540	2	neuropathol	neuropathol	PROPN
fnp-5883	540	3	.	.	PUNCT
fnp-5883	541	1	exp	exp	PROPN
fnp-5883	541	2	.	.	PUNCT
fnp-5883	542	1	neurol	neurol	PROPN
fnp-5883	542	2	.	.	PUNCT
fnp-5883	543	1	35	35	NUM
fnp-5883	543	2	,	,	PUNCT
fnp-5883	543	3	180–90	180–90	NUM
fnp-5883	543	4	.	.	PUNCT
fnp-5883	544	1	https://doi.org/10.1097/00005072-197603000-00005	https://doi.org/10.1097/00005072-197603000-00005	PROPN
fnp-5883	544	2	baraibar	baraibar	PROPN
fnp-5883	544	3	,	,	PUNCT
fnp-5883	544	4	m.a	m.a	PROPN
fnp-5883	544	5	.	.	PROPN
fnp-5883	544	6	,	,	PUNCT
fnp-5883	544	7	schoning	schoning	NOUN
fnp-5883	544	8	,	,	PUNCT
fnp-5883	544	9	p.	p.	PROPN
fnp-5883	544	10	,	,	PUNCT
fnp-5883	544	11	1985	1985	NUM
fnp-5883	544	12	.	.	PUNCT
fnp-5883	545	1	effects	effect	NOUN
fnp-5883	545	2	of	of	ADP
fnp-5883	545	3	freezing	freezing	NOUN
fnp-5883	545	4	and	and	CCONJ
fnp-5883	545	5	frozen	frozen	ADJ
fnp-5883	545	6	storage	storage	NOUN
fnp-5883	545	7	on	on	ADP
fnp-5883	545	8	histological	histological	ADJ
fnp-5883	545	9	characteristics	characteristic	NOUN
fnp-5883	545	10	of	of	ADP
fnp-5883	545	11	canine	canine	NOUN
fnp-5883	545	12	tissues	tissue	NOUN
fnp-5883	545	13	.	.	PUNCT
fnp-5883	546	1	j.	j.	PROPN
fnp-5883	546	2	forensic	forensic	PROPN
fnp-5883	546	3	sci	sci	PROPN
fnp-5883	546	4	.	.	PROPN
fnp-5883	546	5	30	30	NUM
fnp-5883	546	6	,	,	PUNCT
fnp-5883	546	7	439–447	439–447	NUM
fnp-5883	546	8	.	.	PUNCT
fnp-5883	546	9	beach	beach	PROPN
fnp-5883	546	10	,	,	PUNCT
fnp-5883	546	11	t.g	t.g	PROPN
fnp-5883	546	12	.	.	PROPN
fnp-5883	546	13	,	,	PUNCT
fnp-5883	546	14	sue	sue	NOUN
fnp-5883	546	15	,	,	PUNCT
fnp-5883	546	16	l.i	l.i	PROPN
fnp-5883	546	17	.	.	PROPN
fnp-5883	546	18	,	,	PUNCT
fnp-5883	546	19	walker	walker	PROPN
fnp-5883	546	20	,	,	PUNCT
fnp-5883	546	21	d.g	d.g	PROPN
fnp-5883	546	22	.	.	PROPN
fnp-5883	546	23	,	,	PUNCT
fnp-5883	546	24	roher	roher	PROPN
fnp-5883	546	25	,	,	PUNCT
fnp-5883	546	26	a.e	a.e	PROPN
fnp-5883	546	27	.	.	PROPN
fnp-5883	546	28	,	,	PUNCT
fnp-5883	546	29	lue	lue	PROPN
fnp-5883	546	30	,	,	PUNCT
fnp-5883	546	31	l.	l.	PROPN
fnp-5883	546	32	,	,	PUNCT
fnp-5883	546	33	vedders	vedders	PROPN
fnp-5883	546	34	,	,	PUNCT
fnp-5883	546	35	l.	l.	PROPN
fnp-5883	546	36	,	,	PUNCT
fnp-5883	546	37	connor	connor	PROPN
fnp-5883	546	38	,	,	PUNCT
fnp-5883	546	39	d.j	d.j	PROPN
fnp-5883	546	40	.	.	PROPN
fnp-5883	546	41	,	,	PUNCT
fnp-5883	546	42	sabbagh	sabbagh	PROPN
fnp-5883	546	43	,	,	PUNCT
fnp-5883	546	44	m.n	m.n	PROPN
fnp-5883	546	45	.	.	PROPN
fnp-5883	546	46	,	,	PUNCT
fnp-5883	546	47	rogers	rogers	PROPN
fnp-5883	546	48	,	,	PUNCT
fnp-5883	546	49	j.	j.	PROPN
fnp-5883	546	50	,	,	PUNCT
fnp-5883	546	51	2008	2008	NUM
fnp-5883	546	52	.	.	PUNCT
fnp-5883	547	1	the	the	DET
fnp-5883	547	2	sun	sun	PROPN
fnp-5883	547	3	health	health	PROPN
fnp-5883	547	4	research	research	PROPN
fnp-5883	547	5	institute	institute	PROPN
fnp-5883	547	6	brain	brain	PROPN
fnp-5883	547	7	donation	donation	PROPN
fnp-5883	547	8	program	program	NOUN
fnp-5883	547	9	:	:	PUNCT
fnp-5883	547	10	description	description	NOUN
fnp-5883	547	11	and	and	CCONJ
fnp-5883	547	12	eexperience	eexperience	NOUN
fnp-5883	547	13	,	,	PUNCT
fnp-5883	547	14	1987–2007	1987–2007	NUM
fnp-5883	547	15	.	.	PUNCT
fnp-5883	548	1	cell	cell	NOUN
fnp-5883	548	2	tissue	tissue	NOUN
fnp-5883	548	3	bank	bank	NOUN
fnp-5883	548	4	.	.	PUNCT
fnp-5883	549	1	9	9	NUM
fnp-5883	549	2	,	,	PUNCT
fnp-5883	549	3	229–245	229–245	NUM
fnp-5883	549	4	.	.	PUNCT
fnp-5883	550	1	https://doi.org/10.1007/s10561-008-9067-2	https://doi.org/10.1007/s10561-008-9067-2	NUM
fnp-5883	550	2	benson	benson	PROPN
fnp-5883	550	3	,	,	PUNCT
fnp-5883	550	4	t.e	t.e	PROPN
fnp-5883	550	5	.	.	PROPN
fnp-5883	550	6	,	,	PUNCT
fnp-5883	550	7	burd	burd	PROPN
fnp-5883	550	8	,	,	PUNCT
fnp-5883	550	9	g.d	g.d	PROPN
fnp-5883	550	10	.	.	PROPN
fnp-5883	550	11	,	,	PUNCT
fnp-5883	550	12	greer	greer	PROPN
fnp-5883	550	13	,	,	PUNCT
fnp-5883	550	14	c.a	c.a	PROPN
fnp-5883	550	15	.	.	PROPN
fnp-5883	550	16	,	,	PUNCT
fnp-5883	550	17	landis	landis	PROPN
fnp-5883	550	18	,	,	PUNCT
fnp-5883	550	19	d.m	d.m	PROPN
fnp-5883	550	20	.	.	PROPN
fnp-5883	550	21	,	,	PUNCT
fnp-5883	550	22	shepherd	shepherd	PROPN
fnp-5883	550	23	,	,	PUNCT
fnp-5883	550	24	g.m	g.m	PROPN
fnp-5883	550	25	.	.	PROPN
fnp-5883	550	26	,	,	PUNCT
fnp-5883	550	27	1985	1985	NUM
fnp-5883	550	28	.	.	PUNCT
fnp-5883	551	1	high	high	ADJ
fnp-5883	551	2	-	-	PUNCT
fnp-5883	551	3	resolution	resolution	NOUN
fnp-5883	551	4	2	2	NUM
fnp-5883	551	5	-	-	PUNCT
fnp-5883	551	6	deoxyglucose	deoxyglucose	NOUN
fnp-5883	551	7	autoradiography	autoradiography	NOUN
fnp-5883	551	8	in	in	ADP
fnp-5883	551	9	quick	quick	ADJ
fnp-5883	551	10	-	-	PUNCT
fnp-5883	551	11	frozen	frozen	ADJ
fnp-5883	551	12	slabs	slab	NOUN
fnp-5883	551	13	of	of	ADP
fnp-5883	551	14	neonatal	neonatal	ADJ
fnp-5883	551	15	rat	rat	NOUN
fnp-5883	551	16	olfactory	olfactory	NOUN
fnp-5883	551	17	bulb	bulb	PROPN
fnp-5883	551	18	.	.	PUNCT
fnp-5883	552	1	brain	brain	NOUN
fnp-5883	552	2	res	re	NOUN
fnp-5883	552	3	.	.	PUNCT
fnp-5883	552	4	339	339	NUM
fnp-5883	552	5	,	,	PUNCT
fnp-5883	552	6	67–78	67–78	NOUN
fnp-5883	552	7	.	.	PUNCT
fnp-5883	553	1	https://doi.org/10.1016/0006-8993(85)90622-5	https://doi.org/10.1016/0006-8993(85)90622-5	ADV
fnp-5883	553	2	best	well	ADV
fnp-5883	553	3	,	,	PUNCT
fnp-5883	553	4	b.p	b.p	PROPN
fnp-5883	553	5	.	.	PROPN
fnp-5883	553	6	,	,	PUNCT
fnp-5883	553	7	2008	2008	NUM
fnp-5883	553	8	.	.	PUNCT
fnp-5883	554	1	scientific	scientific	ADJ
fnp-5883	554	2	justification	justification	NOUN
fnp-5883	554	3	of	of	ADP
fnp-5883	554	4	cryonics	cryonic	NOUN
fnp-5883	554	5	practice	practice	NOUN
fnp-5883	554	6	.	.	PUNCT
fnp-5883	555	1	rejuvenation	rejuvenation	NOUN
fnp-5883	555	2	res	re	NOUN
fnp-5883	555	3	.	.	PUNCT
fnp-5883	556	1	11	11	NUM
fnp-5883	556	2	,	,	PUNCT
fnp-5883	556	3	493–503	493–503	NUM
fnp-5883	556	4	.	.	PUNCT
fnp-5883	557	1	https://doi.org/10.1089/rej.2008.0661	https://doi.org/10.1089/rej.2008.0661	PROPN
fnp-5883	557	2	bird	bird	NOUN
fnp-5883	557	3	,	,	PUNCT
fnp-5883	557	4	e.	e.	PROPN
fnp-5883	557	5	,	,	PUNCT
fnp-5883	557	6	1980	1980	NUM
fnp-5883	557	7	.	.	PUNCT
fnp-5883	558	1	a	a	DET
fnp-5883	558	2	brain	brain	NOUN
fnp-5883	558	3	tissue	tissue	NOUN
fnp-5883	558	4	resource	resource	NOUN
fnp-5883	558	5	center	center	NOUN
fnp-5883	558	6	to	to	PART
fnp-5883	558	7	promote	promote	VERB
fnp-5883	558	8	research	research	NOUN
fnp-5883	558	9	in	in	ADP
fnp-5883	558	10	schizophrenia	schizophrenia	NOUN
fnp-5883	558	11	.	.	PUNCT
fnp-5883	559	1	perspect	perspect	VERB
fnp-5883	559	2	.	.	PUNCT
fnp-5883	560	1	schizophr	schizophr	PROPN
fnp-5883	560	2	.	.	PUNCT
fnp-5883	561	1	res	re	NOUN
fnp-5883	561	2	.	.	PUNCT
fnp-5883	561	3	raven	raven	PROPN
fnp-5883	561	4	n.	n.	PROPN
fnp-5883	561	5	y.	y.	PROPN
fnp-5883	561	6	bojic	bojic	PROPN
fnp-5883	561	7	,	,	PUNCT
fnp-5883	561	8	s.	s.	PROPN
fnp-5883	561	9	,	,	PUNCT
fnp-5883	561	10	murray	murray	PROPN
fnp-5883	561	11	,	,	PUNCT
fnp-5883	561	12	a.	a.	PROPN
fnp-5883	561	13	,	,	PUNCT
fnp-5883	561	14	bentley	bentley	PROPN
fnp-5883	561	15	,	,	PUNCT
fnp-5883	561	16	b.l	b.l	PROPN
fnp-5883	561	17	.	.	PROPN
fnp-5883	561	18	,	,	PUNCT
fnp-5883	561	19	spindler	spindler	PROPN
fnp-5883	561	20	,	,	PUNCT
fnp-5883	561	21	r.	r.	PROPN
fnp-5883	561	22	,	,	PUNCT
fnp-5883	561	23	pawlik	pawlik	NOUN
fnp-5883	561	24	,	,	PUNCT
fnp-5883	561	25	p.	p.	PROPN
fnp-5883	561	26	,	,	PUNCT
fnp-5883	561	27	cordeiro	cordeiro	PROPN
fnp-5883	561	28	,	,	PUNCT
fnp-5883	561	29	j.l	j.l	PROPN
fnp-5883	561	30	.	.	PROPN
fnp-5883	561	31	,	,	PUNCT
fnp-5883	561	32	bauer	bauer	PROPN
fnp-5883	561	33	,	,	PUNCT
fnp-5883	561	34	r.	r.	PROPN
fnp-5883	561	35	,	,	PUNCT
fnp-5883	561	36	de	de	X
fnp-5883	561	37	magalhães	magalhães	PROPN
fnp-5883	561	38	,	,	PUNCT
fnp-5883	561	39	j.p	j.p	PROPN
fnp-5883	561	40	.	.	PROPN
fnp-5883	561	41	,	,	PUNCT
fnp-5883	561	42	2021	2021	NUM
fnp-5883	561	43	.	.	PUNCT
fnp-5883	562	1	winter	winter	NOUN
fnp-5883	562	2	is	be	AUX
fnp-5883	562	3	coming	come	VERB
fnp-5883	562	4	:	:	PUNCT
fnp-5883	562	5	the	the	DET
fnp-5883	562	6	future	future	NOUN
fnp-5883	562	7	of	of	ADP
fnp-5883	562	8	cryopreservation	cryopreservation	NOUN
fnp-5883	562	9	.	.	PUNCT
fnp-5883	563	1	bmc	bmc	PROPN
fnp-5883	563	2	biol	biol	PROPN
fnp-5883	563	3	.	.	PUNCT
fnp-5883	564	1	19	19	NUM
fnp-5883	564	2	,	,	PUNCT
fnp-5883	564	3	56	56	NUM
fnp-5883	564	4	.	.	PUNCT
fnp-5883	564	5	https://doi.org/10.1186/s12915-021-00976-8	https://doi.org/10.1186/s12915-021-00976-8	PROPN
fnp-5883	564	6	borges	borge	NOUN
fnp-5883	564	7	-	-	PUNCT
fnp-5883	564	8	merjane	merjane	ADJ
fnp-5883	564	9	,	,	PUNCT
fnp-5883	564	10	c.	c.	PROPN
fnp-5883	564	11	,	,	PUNCT
fnp-5883	564	12	kim	kim	PROPN
fnp-5883	564	13	,	,	PUNCT
fnp-5883	564	14	o.	o.	PROPN
fnp-5883	564	15	,	,	PUNCT
fnp-5883	564	16	jonas	jonas	PROPN
fnp-5883	564	17	,	,	PUNCT
fnp-5883	564	18	p.	p.	PROPN
fnp-5883	564	19	,	,	PUNCT
fnp-5883	564	20	2020	2020	NUM
fnp-5883	564	21	.	.	PUNCT
fnp-5883	565	1	functional	functional	ADJ
fnp-5883	565	2	electron	electron	NOUN
fnp-5883	565	3	microscopy	microscopy	NOUN
fnp-5883	565	4	,	,	PUNCT
fnp-5883	565	5	“	"	PUNCT
fnp-5883	565	6	flash	flash	VERB
fnp-5883	565	7	and	and	CCONJ
fnp-5883	565	8	freeze	freeze	VERB
fnp-5883	565	9	,	,	PUNCT
fnp-5883	565	10	”	"	PUNCT
fnp-5883	565	11	of	of	ADP
fnp-5883	565	12	identified	identify	VERB
fnp-5883	565	13	cortical	cortical	ADJ
fnp-5883	565	14	synapses	synapsis	NOUN
fnp-5883	565	15	in	in	ADP
fnp-5883	565	16	acute	acute	ADJ
fnp-5883	565	17	brain	brain	NOUN
fnp-5883	565	18	slices	slice	NOUN
fnp-5883	565	19	.	.	PUNCT
fnp-5883	566	1	neuron	neuron	PROPN
fnp-5883	566	2	105	105	NUM
fnp-5883	566	3	,	,	PUNCT
fnp-5883	566	4	992	992	NUM
fnp-5883	566	5	-	-	PUNCT
fnp-5883	566	6	1006.e6	1006.e6	NUM
fnp-5883	566	7	.	.	PUNCT
fnp-5883	567	1	https://doi.org/10.1016/j.neuron.2019.12.022	https://doi.org/10.1016/j.neuron.2019.12.022	NOUN
fnp-5883	567	2	cahill	cahill	NOUN
fnp-5883	567	3	,	,	PUNCT
fnp-5883	567	4	l.s	l.s	PROPN
fnp-5883	567	5	.	.	PROPN
fnp-5883	567	6	,	,	PUNCT
fnp-5883	567	7	laliberté	laliberté	PROPN
fnp-5883	567	8	,	,	PUNCT
fnp-5883	567	9	c.l	c.l	PROPN
fnp-5883	567	10	.	.	PROPN
fnp-5883	567	11	,	,	PUNCT
fnp-5883	567	12	ellegood	ellegood	PROPN
fnp-5883	567	13	,	,	PUNCT
fnp-5883	567	14	j.	j.	PROPN
fnp-5883	567	15	,	,	PUNCT
fnp-5883	567	16	spring	spring	PROPN
fnp-5883	567	17	,	,	PUNCT
fnp-5883	567	18	s.	s.	PROPN
fnp-5883	567	19	,	,	PUNCT
fnp-5883	567	20	gleave	gleave	PROPN
fnp-5883	567	21	,	,	PUNCT
fnp-5883	567	22	j.a	j.a	PROPN
fnp-5883	567	23	.	.	PROPN
fnp-5883	567	24	,	,	PUNCT
fnp-5883	567	25	eede	eede	PROPN
fnp-5883	567	26	,	,	PUNCT
fnp-5883	567	27	m.c	m.c	PROPN
fnp-5883	567	28	.	.	PROPN
fnp-5883	567	29	van	van	PROPN
fnp-5883	567	30	,	,	PUNCT
fnp-5883	567	31	lerch	lerch	PROPN
fnp-5883	567	32	,	,	PUNCT
fnp-5883	567	33	j.p	j.p	PROPN
fnp-5883	567	34	.	.	PROPN
fnp-5883	567	35	,	,	PUNCT
fnp-5883	567	36	henkelman	henkelman	PROPN
fnp-5883	567	37	,	,	PUNCT
fnp-5883	567	38	r.m	r.m	PROPN
fnp-5883	567	39	.	.	PROPN
fnp-5883	567	40	,	,	PUNCT
fnp-5883	567	41	2012	2012	NUM
fnp-5883	567	42	.	.	PUNCT
fnp-5883	568	1	preparation	preparation	NOUN
fnp-5883	568	2	of	of	ADP
fnp-5883	568	3	fixed	fix	VERB
fnp-5883	568	4	mouse	mouse	NOUN
fnp-5883	568	5	brains	brain	NOUN
fnp-5883	568	6	for	for	ADP
fnp-5883	568	7	mri	mri	NOUN
fnp-5883	568	8	.	.	PUNCT
fnp-5883	569	1	neuroimage	neuroimage	NOUN
fnp-5883	569	2	60	60	NUM
fnp-5883	569	3	,	,	PUNCT
fnp-5883	569	4	933–939	933–939	NUM
fnp-5883	569	5	.	.	PUNCT
fnp-5883	570	1	https://doi.org/10.1016/j.neuroimage.2012.01.100	https://doi.org/10.1016/j.neuroimage.2012.01.100	PROPN
fnp-5883	570	2	canatelli	canatelli	PROPN
fnp-5883	570	3	-	-	PUNCT
fnp-5883	570	4	mallat	mallat	NOUN
fnp-5883	570	5	,	,	PUNCT
fnp-5883	570	6	m.	m.	NOUN
fnp-5883	570	7	,	,	PUNCT
fnp-5883	570	8	lascaray	lascaray	NOUN
fnp-5883	570	9	,	,	PUNCT
fnp-5883	570	10	f.	f.	PROPN
fnp-5883	570	11	,	,	PUNCT
fnp-5883	570	12	entraigues	entraigues	PROPN
fnp-5883	570	13	-	-	PUNCT
fnp-5883	570	14	abramson	abramson	PROPN
fnp-5883	570	15	,	,	PUNCT
fnp-5883	570	16	m.	m.	NOUN
fnp-5883	570	17	,	,	PUNCT
fnp-5883	570	18	portiansky	portiansky	NOUN
fnp-5883	570	19	,	,	PUNCT
fnp-5883	570	20	e.l	e.l	PROPN
fnp-5883	570	21	.	.	PROPN
fnp-5883	570	22	,	,	PUNCT
fnp-5883	570	23	blamaceda	blamaceda	PROPN
fnp-5883	570	24	,	,	PUNCT
fnp-5883	570	25	n.	n.	NOUN
fnp-5883	570	26	,	,	PUNCT
fnp-5883	570	27	morel	morel	PROPN
fnp-5883	570	28	,	,	PUNCT
fnp-5883	570	29	g.r	g.r	PROPN
fnp-5883	570	30	.	.	PROPN
fnp-5883	570	31	,	,	PUNCT
fnp-5883	570	32	goya	goya	PROPN
fnp-5883	570	33	,	,	PUNCT
fnp-5883	570	34	r.g	r.g	PROPN
fnp-5883	570	35	.	.	PROPN
fnp-5883	570	36	,	,	PUNCT
fnp-5883	570	37	2020	2020	NUM
fnp-5883	570	38	.	.	PUNCT
fnp-5883	571	1	cryopreservation	cryopreservation	NOUN
fnp-5883	571	2	of	of	ADP
fnp-5883	571	3	a	a	DET
fnp-5883	571	4	human	human	ADJ
fnp-5883	571	5	brain	brain	NOUN
fnp-5883	571	6	and	and	CCONJ
fnp-5883	571	7	its	its	PRON
fnp-5883	571	8	experimental	experimental	ADJ
fnp-5883	571	9	correlate	correlate	NOUN
fnp-5883	571	10	in	in	ADP
fnp-5883	571	11	rats	rat	NOUN
fnp-5883	571	12	.	.	PUNCT
fnp-5883	572	1	rejuvenation	rejuvenation	NOUN
fnp-5883	572	2	res	re	NOUN
fnp-5883	572	3	.	.	PROPN
fnp-5883	573	1	23	23	NUM
fnp-5883	573	2	,	,	PUNCT
fnp-5883	573	3	516–525	516–525	NUM
fnp-5883	573	4	.	.	PUNCT
fnp-5883	574	1	https://doi.org/10.1089/rej.2019.2245	https://doi.org/10.1089/rej.2019.2245	PROPN
fnp-5883	574	2	cao	cao	PROPN
fnp-5883	574	3	,	,	PUNCT
fnp-5883	574	4	e.	e.	PROPN
fnp-5883	574	5	,	,	PUNCT
fnp-5883	574	6	chen	chen	PROPN
fnp-5883	574	7	,	,	PUNCT
fnp-5883	574	8	y.	y.	PROPN
fnp-5883	574	9	,	,	PUNCT
fnp-5883	574	10	cui	cui	PROPN
fnp-5883	574	11	,	,	PUNCT
fnp-5883	574	12	z.	z.	PROPN
fnp-5883	574	13	,	,	PUNCT
fnp-5883	574	14	foster	foster	PROPN
fnp-5883	574	15	,	,	PUNCT
fnp-5883	574	16	p.r	p.r	PROPN
fnp-5883	574	17	.	.	PROPN
fnp-5883	574	18	,	,	PUNCT
fnp-5883	574	19	2003	2003	NUM
fnp-5883	574	20	.	.	PUNCT
fnp-5883	575	1	effect	effect	NOUN
fnp-5883	575	2	of	of	ADP
fnp-5883	575	3	freezing	freeze	VERB
fnp-5883	575	4	and	and	CCONJ
fnp-5883	575	5	thawing	thaw	VERB
fnp-5883	575	6	rates	rate	NOUN
fnp-5883	575	7	on	on	ADP
fnp-5883	575	8	denaturation	denaturation	NOUN
fnp-5883	575	9	of	of	ADP
fnp-5883	575	10	proteins	protein	NOUN
fnp-5883	575	11	in	in	ADP
fnp-5883	575	12	aqueous	aqueous	ADJ
fnp-5883	575	13	solutions	solution	NOUN
fnp-5883	575	14	.	.	PUNCT
fnp-5883	576	1	biotechnol	biotechnol	NOUN
fnp-5883	576	2	.	.	PUNCT
fnp-5883	577	1	bioeng	bioeng	PROPN
fnp-5883	577	2	.	.	PROPN
fnp-5883	578	1	82	82	NUM
fnp-5883	578	2	,	,	PUNCT
fnp-5883	578	3	684–690	684–690	NUM
fnp-5883	578	4	.	.	PUNCT
fnp-5883	579	1	https://doi.org/10.1002/bit.10612	https://doi.org/10.1002/bit.10612	PROPN
fnp-5883	579	2	chambers	chambers	PROPN
fnp-5883	579	3	,	,	PUNCT
fnp-5883	579	4	r.	r.	PROPN
fnp-5883	579	5	,	,	PUNCT
fnp-5883	579	6	hale	hale	PROPN
fnp-5883	579	7	,	,	PUNCT
fnp-5883	579	8	h.p	h.p	PROPN
fnp-5883	579	9	.	.	PROPN
fnp-5883	579	10	,	,	PUNCT
fnp-5883	579	11	hardy	hardy	ADJ
fnp-5883	579	12	,	,	PUNCT
fnp-5883	579	13	w.b	w.b	PROPN
fnp-5883	579	14	.	.	PROPN
fnp-5883	579	15	,	,	PUNCT
fnp-5883	579	16	1932	1932	NUM
fnp-5883	579	17	.	.	PUNCT
fnp-5883	580	1	the	the	DET
fnp-5883	580	2	formation	formation	NOUN
fnp-5883	580	3	of	of	ADP
fnp-5883	580	4	ice	ice	NOUN
fnp-5883	580	5	in	in	ADP
fnp-5883	580	6	protoplasm	protoplasm	NOUN
fnp-5883	580	7	.	.	PUNCT
fnp-5883	581	1	proc	proc	PROPN
fnp-5883	581	2	.	.	PUNCT
fnp-5883	582	1	r.	r.	PROPN
fnp-5883	582	2	soc	soc	PROPN
fnp-5883	582	3	.	.	PUNCT
fnp-5883	583	1	lond	lond	PROPN
fnp-5883	583	2	.	.	PUNCT
fnp-5883	584	1	ser	ser	PROPN
fnp-5883	584	2	.	.	PUNCT
fnp-5883	585	1	b	b	NOUN
fnp-5883	585	2	contain	contain	NOUN
fnp-5883	585	3	.	.	PUNCT
fnp-5883	586	1	pap	pap	NOUN
fnp-5883	586	2	.	.	PUNCT
fnp-5883	587	1	biol	biol	PROPN
fnp-5883	587	2	.	.	PUNCT
fnp-5883	588	1	character	character	NOUN
fnp-5883	588	2	110	110	NUM
fnp-5883	588	3	,	,	PUNCT
fnp-5883	588	4	336–352	336–352	NUM
fnp-5883	588	5	.	.	PUNCT
fnp-5883	589	1	https://doi.org/10.1098/rspb.1932.0029	https://doi.org/10.1098/rspb.1932.0029	PROPN
fnp-5883	589	2	choi	choi	NOUN
fnp-5883	589	3	,	,	PUNCT
fnp-5883	589	4	y.-s	y.-	NOUN
fnp-5883	589	5	.	.	PUNCT
fnp-5883	589	6	,	,	PUNCT
fnp-5883	589	7	ku	ku	PROPN
fnp-5883	589	8	,	,	PUNCT
fnp-5883	589	9	s.-k	s.-k	PROPN
fnp-5883	589	10	.	.	PROPN
fnp-5883	589	11	,	,	PUNCT
fnp-5883	589	12	jeong	jeong	PROPN
fnp-5883	589	13	,	,	PUNCT
fnp-5883	589	14	j.-y	j.-y	PROPN
fnp-5883	589	15	.	.	PROPN
fnp-5883	589	16	,	,	PUNCT
fnp-5883	589	17	jeon	jeon	PROPN
fnp-5883	589	18	,	,	PUNCT
fnp-5883	589	19	k.-h	k.-h	PROPN
fnp-5883	589	20	.	.	PUNCT
fnp-5883	589	21	,	,	PUNCT
fnp-5883	589	22	kim	kim	PROPN
fnp-5883	589	23	,	,	PUNCT
fnp-5883	589	24	y.-b	y.-b	PROPN
fnp-5883	589	25	.	.	PROPN
fnp-5883	589	26	,	,	PUNCT
fnp-5883	589	27	2015	2015	NUM
fnp-5883	589	28	.	.	PUNCT
fnp-5883	590	1	changes	change	NOUN
fnp-5883	590	2	in	in	ADP
fnp-5883	590	3	ultrastructure	ultrastructure	ADJ
fnp-5883	590	4	and	and	CCONJ
fnp-5883	590	5	sensory	sensory	ADJ
fnp-5883	590	6	characteristics	characteristic	NOUN
fnp-5883	590	7	on	on	ADP
fnp-5883	590	8	electro	electro	NOUN
fnp-5883	590	9	-	-	PUNCT
fnp-5883	590	10	magnetic	magnetic	ADJ
fnp-5883	590	11	and	and	CCONJ
fnp-5883	590	12	air	air	NOUN
fnp-5883	590	13	blast	blast	NOUN
fnp-5883	590	14	freezing	freezing	NOUN
fnp-5883	590	15	of	of	ADP
fnp-5883	590	16	beef	beef	NOUN
fnp-5883	590	17	during	during	ADP
fnp-5883	590	18	frozen	frozen	ADJ
fnp-5883	590	19	storage	storage	NOUN
fnp-5883	590	20	.	.	PUNCT
fnp-5883	591	1	korean	korean	ADJ
fnp-5883	591	2	j.	j.	PROPN
fnp-5883	591	3	food	food	PROPN
fnp-5883	591	4	sci	sci	PROPN
fnp-5883	591	5	.	.	PUNCT
fnp-5883	591	6	anim	anim	PROPN
fnp-5883	591	7	.	.	PUNCT
fnp-5883	592	1	resour	resour	NOUN
fnp-5883	592	2	.	.	PROPN
fnp-5883	593	1	35	35	NUM
fnp-5883	593	2	,	,	PUNCT
fnp-5883	593	3	27–34	27–34	NUM
fnp-5883	593	4	.	.	PUNCT
fnp-5883	593	5	https://doi.org/10.5851/kosfa.2015.35.1.27	https://doi.org/10.5851/kosfa.2015.35.1.27	PROPN
fnp-5883	593	6	clarke	clarke	PROPN
fnp-5883	593	7	,	,	PUNCT
fnp-5883	593	8	a.	a.	PROPN
fnp-5883	593	9	,	,	PUNCT
fnp-5883	593	10	morris	morris	PROPN
fnp-5883	593	11	,	,	PUNCT
fnp-5883	593	12	g.j	g.j	PROPN
fnp-5883	593	13	.	.	PROPN
fnp-5883	593	14	,	,	PUNCT
fnp-5883	593	15	fonseca	fonseca	PROPN
fnp-5883	593	16	,	,	PUNCT
fnp-5883	593	17	f.	f.	PROPN
fnp-5883	593	18	,	,	PUNCT
fnp-5883	593	19	murray	murray	PROPN
fnp-5883	593	20	,	,	PUNCT
fnp-5883	593	21	b.j	b.j	PROPN
fnp-5883	593	22	.	.	PROPN
fnp-5883	593	23	,	,	PUNCT
fnp-5883	593	24	acton	acton	PROPN
fnp-5883	593	25	,	,	PUNCT
fnp-5883	593	26	e.	e.	PROPN
fnp-5883	593	27	,	,	PUNCT
fnp-5883	593	28	price	price	NOUN
fnp-5883	593	29	,	,	PUNCT
fnp-5883	593	30	h.c	h.c	PROPN
fnp-5883	593	31	.	.	PROPN
fnp-5883	593	32	,	,	PUNCT
fnp-5883	593	33	2013	2013	NUM
fnp-5883	593	34	.	.	PUNCT
fnp-5883	594	1	a	a	DET
fnp-5883	594	2	low	low	ADJ
fnp-5883	594	3	temperature	temperature	NOUN
fnp-5883	594	4	limit	limit	NOUN
fnp-5883	594	5	for	for	ADP
fnp-5883	594	6	life	life	NOUN
fnp-5883	594	7	on	on	ADP
fnp-5883	594	8	earth	earth	NOUN
fnp-5883	594	9	.	.	PUNCT
fnp-5883	595	1	plos	plos	PROPN
fnp-5883	595	2	one	one	NUM
fnp-5883	595	3	8	8	NUM
fnp-5883	595	4	,	,	PUNCT
fnp-5883	595	5	e66207	e66207	NOUN
fnp-5883	595	6	.	.	PUNCT
fnp-5883	596	1	https://doi.org/10.1371/journal.pone.0066207	https://doi.org/10.1371/journal.pone.0066207	PROPN
fnp-5883	596	2	comba	comba	PROPN
fnp-5883	596	3	,	,	PUNCT
fnp-5883	596	4	a.	a.	PROPN
fnp-5883	596	5	,	,	PUNCT
fnp-5883	596	6	dunn	dunn	PROPN
fnp-5883	596	7	,	,	PUNCT
fnp-5883	596	8	p.j	p.j	PROPN
fnp-5883	596	9	.	.	PROPN
fnp-5883	596	10	,	,	PUNCT
fnp-5883	596	11	kish	kish	PROPN
fnp-5883	596	12	,	,	PUNCT
fnp-5883	596	13	p.e	p.e	PROPN
fnp-5883	596	14	.	.	PROPN
fnp-5883	596	15	,	,	PUNCT
fnp-5883	596	16	kadiyala	kadiyala	PROPN
fnp-5883	596	17	,	,	PUNCT
fnp-5883	596	18	p.	p.	PROPN
fnp-5883	596	19	,	,	PUNCT
fnp-5883	596	20	kahana	kahana	PROPN
fnp-5883	596	21	,	,	PUNCT
fnp-5883	596	22	a.	a.	PROPN
fnp-5883	596	23	,	,	PUNCT
fnp-5883	596	24	castro	castro	PROPN
fnp-5883	596	25	,	,	PUNCT
fnp-5883	596	26	m.g	m.g	PROPN
fnp-5883	596	27	.	.	PROPN
fnp-5883	596	28	,	,	PUNCT
fnp-5883	596	29	lowenstein	lowenstein	PROPN
fnp-5883	596	30	,	,	PUNCT
fnp-5883	596	31	p.r	p.r	PROPN
fnp-5883	596	32	.	.	PROPN
fnp-5883	596	33	,	,	PUNCT
fnp-5883	596	34	2020	2020	NUM
fnp-5883	596	35	.	.	PUNCT
fnp-5883	597	1	laser	laser	NOUN
fnp-5883	597	2	capture	capture	NOUN
fnp-5883	597	3	microdissection	microdissection	NOUN
fnp-5883	597	4	of	of	ADP
fnp-5883	597	5	glioma	glioma	NOUN
fnp-5883	597	6	subregions	subregion	NOUN
fnp-5883	597	7	for	for	ADP
fnp-5883	597	8	spatial	spatial	ADJ
fnp-5883	597	9	and	and	CCONJ
fnp-5883	597	10	molecular	molecular	ADJ
fnp-5883	597	11	characterization	characterization	NOUN
fnp-5883	597	12	of	of	ADP
fnp-5883	597	13	intratumoral	intratumoral	ADJ
fnp-5883	597	14	heterogeneity	heterogeneity	NOUN
fnp-5883	597	15	,	,	PUNCT
fnp-5883	597	16	oncostreams	oncostream	NOUN
fnp-5883	597	17	,	,	PUNCT
fnp-5883	597	18	and	and	CCONJ
fnp-5883	597	19	invasion	invasion	NOUN
fnp-5883	597	20	.	.	PUNCT
fnp-5883	598	1	j.	j.	PROPN
fnp-5883	598	2	vis	vis	PROPN
fnp-5883	598	3	.	.	PUNCT
fnp-5883	598	4	exp	exp	PROPN
fnp-5883	598	5	.	.	PUNCT
fnp-5883	598	6	jove	jove	PROPN
fnp-5883	598	7	.	.	PUNCT
fnp-5883	598	8	https://doi.org/10.3791/60939	https://doi.org/10.3791/60939	X
fnp-5883	599	1	das	das	PROPN
fnp-5883	599	2	,	,	PUNCT
fnp-5883	599	3	g.d	g.d	PROPN
fnp-5883	599	4	.	.	PROPN
fnp-5883	599	5	,	,	PUNCT
fnp-5883	599	6	houle	houle	PROPN
fnp-5883	599	7	,	,	PUNCT
fnp-5883	599	8	j.d	j.d	PROPN
fnp-5883	599	9	.	.	PROPN
fnp-5883	599	10	,	,	PUNCT
fnp-5883	599	11	brasko	brasko	PROPN
fnp-5883	599	12	,	,	PUNCT
fnp-5883	599	13	j.	j.	PROPN
fnp-5883	599	14	,	,	PUNCT
fnp-5883	599	15	das	das	PROPN
fnp-5883	599	16	,	,	PUNCT
fnp-5883	599	17	k.g	k.g	PROPN
fnp-5883	599	18	.	.	PROPN
fnp-5883	599	19	,	,	PUNCT
fnp-5883	599	20	1983	1983	NUM
fnp-5883	599	21	.	.	PUNCT
fnp-5883	600	1	freezing	freezing	NOUN
fnp-5883	600	2	of	of	ADP
fnp-5883	600	3	neural	neural	ADJ
fnp-5883	600	4	tissues	tissue	NOUN
fnp-5883	600	5	and	and	CCONJ
fnp-5883	600	6	their	their	PRON
fnp-5883	600	7	transplantation	transplantation	NOUN
fnp-5883	600	8	in	in	ADP
fnp-5883	600	9	the	the	DET
fnp-5883	600	10	brain	brain	NOUN
fnp-5883	600	11	of	of	ADP
fnp-5883	600	12	rats	rat	NOUN
fnp-5883	600	13	:	:	PUNCT
fnp-5883	600	14	technical	technical	ADJ
fnp-5883	600	15	details	detail	NOUN
fnp-5883	600	16	and	and	CCONJ
fnp-5883	600	17	histological	histological	ADJ
fnp-5883	600	18	observations	observation	NOUN
fnp-5883	600	19	.	.	PUNCT
fnp-5883	601	1	j.	j.	PROPN
fnp-5883	601	2	neurosci	neurosci	PROPN
fnp-5883	601	3	.	.	PUNCT
fnp-5883	602	1	methods	method	NOUN
fnp-5883	602	2	8	8	NUM
fnp-5883	602	3	,	,	PUNCT
fnp-5883	602	4	1–15	1–15	NUM
fnp-5883	602	5	.	.	PUNCT
fnp-5883	603	1	https://doi.org/10.1016/0165-0270(83)90047-x	https://doi.org/10.1016/0165-0270(83)90047-x	PROPN
fnp-5883	603	2	davis	davis	PROPN
fnp-5883	603	3	,	,	PUNCT
fnp-5883	603	4	s.	s.	PROPN
fnp-5883	603	5	,	,	PUNCT
fnp-5883	603	6	scott	scott	PROPN
fnp-5883	603	7	,	,	PUNCT
fnp-5883	603	8	c.	c.	PROPN
fnp-5883	603	9	,	,	PUNCT
fnp-5883	603	10	ansorge	ansorge	NOUN
fnp-5883	603	11	,	,	PUNCT
fnp-5883	603	12	o.	o.	PROPN
fnp-5883	603	13	,	,	PUNCT
fnp-5883	603	14	fischer	fischer	PROPN
fnp-5883	603	15	,	,	PUNCT
fnp-5883	603	16	r.	r.	PROPN
fnp-5883	603	17	,	,	PUNCT
fnp-5883	603	18	2019	2019	NUM
fnp-5883	603	19	.	.	PUNCT
fnp-5883	604	1	development	development	NOUN
fnp-5883	604	2	of	of	ADP
fnp-5883	604	3	a	a	DET
fnp-5883	604	4	sensitive	sensitive	ADJ
fnp-5883	604	5	,	,	PUNCT
fnp-5883	604	6	scalable	scalable	ADJ
fnp-5883	604	7	method	method	NOUN
fnp-5883	604	8	for	for	ADP
fnp-5883	604	9	spatial	spatial	ADJ
fnp-5883	604	10	,	,	PUNCT
fnp-5883	604	11	cell	cell	NOUN
fnp-5883	604	12	-	-	PUNCT
fnp-5883	604	13	type	type	NOUN
fnp-5883	604	14	-	-	PUNCT
fnp-5883	604	15	resolved	resolve	VERB
fnp-5883	604	16	proteomics	proteomic	NOUN
fnp-5883	604	17	of	of	ADP
fnp-5883	604	18	the	the	DET
fnp-5883	604	19	human	human	ADJ
fnp-5883	604	20	brain	brain	NOUN
fnp-5883	604	21	.	.	PUNCT
fnp-5883	605	1	j.	j.	PROPN
fnp-5883	605	2	proteome	proteome	PROPN
fnp-5883	605	3	res	res	PROPN
fnp-5883	605	4	.	.	PROPN
fnp-5883	605	5	18	18	NUM
fnp-5883	605	6	,	,	PUNCT
fnp-5883	605	7	1787–1795	1787–1795	NUM
fnp-5883	605	8	.	.	PUNCT
fnp-5883	606	1	https://doi.org/10.1021/acs.jproteome.8b00981	https://doi.org/10.1021/acs.jproteome.8b00981	NOUN
fnp-5883	606	2	dumoux	dumoux	NOUN
fnp-5883	606	3	,	,	PUNCT
fnp-5883	606	4	m.	m.	NOUN
fnp-5883	606	5	,	,	PUNCT
fnp-5883	606	6	glen	glen	PROPN
fnp-5883	606	7	,	,	PUNCT
fnp-5883	606	8	t.	t.	PROPN
fnp-5883	606	9	,	,	PUNCT
fnp-5883	606	10	smith	smith	PROPN
fnp-5883	606	11	,	,	PUNCT
fnp-5883	606	12	j.l.r	j.l.r	NOUN
fnp-5883	606	13	.	.	PROPN
fnp-5883	606	14	,	,	PUNCT
fnp-5883	606	15	ho	ho	PROPN
fnp-5883	606	16	,	,	PUNCT
fnp-5883	606	17	e.m.l	e.m.l	NOUN
fnp-5883	606	18	.	.	PROPN
fnp-5883	606	19	,	,	PUNCT
fnp-5883	606	20	perdigão	perdigão	PROPN
fnp-5883	606	21	,	,	PUNCT
fnp-5883	606	22	l.m.a	l.m.a	PROPN
fnp-5883	606	23	.	.	PROPN
fnp-5883	606	24	,	,	PUNCT
fnp-5883	606	25	pennington	pennington	PROPN
fnp-5883	606	26	,	,	PUNCT
fnp-5883	606	27	a.	a.	PROPN
fnp-5883	606	28	,	,	PUNCT
fnp-5883	606	29	klumpe	klumpe	PROPN
fnp-5883	606	30	,	,	PUNCT
fnp-5883	606	31	s.	s.	PROPN
fnp-5883	606	32	,	,	PUNCT
fnp-5883	606	33	yee	yee	PROPN
fnp-5883	606	34	,	,	PUNCT
fnp-5883	606	35	n.b.y	n.b.y	PROPN
fnp-5883	606	36	.	.	PROPN
fnp-5883	606	37	,	,	PUNCT
fnp-5883	606	38	farmer	farmer	NOUN
fnp-5883	606	39	,	,	PUNCT
fnp-5883	606	40	d.a	d.a	PROPN
fnp-5883	606	41	.	.	PROPN
fnp-5883	606	42	,	,	PUNCT
fnp-5883	606	43	lai	lai	PROPN
fnp-5883	606	44	,	,	PUNCT
fnp-5883	606	45	p.y.a	p.y.a	NOUN
fnp-5883	606	46	.	.	PUNCT
fnp-5883	606	47	,	,	PUNCT
fnp-5883	606	48	bowles	bowles	PROPN
fnp-5883	606	49	,	,	PUNCT
fnp-5883	606	50	w.	w.	PROPN
fnp-5883	606	51	,	,	PUNCT
fnp-5883	606	52	kelley	kelley	PROPN
fnp-5883	606	53	,	,	PUNCT
fnp-5883	606	54	r.	r.	PROPN
fnp-5883	606	55	,	,	PUNCT
fnp-5883	606	56	plitzko	plitzko	NOUN
fnp-5883	606	57	,	,	PUNCT
fnp-5883	606	58	j.m	j.m	PROPN
fnp-5883	606	59	.	.	PROPN
fnp-5883	606	60	,	,	PUNCT
fnp-5883	606	61	wu	wu	PROPN
fnp-5883	606	62	,	,	PUNCT
fnp-5883	606	63	l.	l.	PROPN
fnp-5883	606	64	,	,	PUNCT
fnp-5883	606	65	basham	basham	PROPN
fnp-5883	606	66	,	,	PUNCT
fnp-5883	606	67	m.	m.	NOUN
fnp-5883	606	68	,	,	PUNCT
fnp-5883	606	69	clare	clare	NOUN
fnp-5883	606	70	,	,	PUNCT
fnp-5883	606	71	d.k	d.k	PROPN
fnp-5883	606	72	.	.	PROPN
fnp-5883	606	73	,	,	PUNCT
fnp-5883	606	74	siebert	siebert	PROPN
fnp-5883	606	75	,	,	PUNCT
fnp-5883	606	76	c.a	c.a	PROPN
fnp-5883	606	77	.	.	PROPN
fnp-5883	606	78	,	,	PUNCT
fnp-5883	606	79	darrow	darrow	PROPN
fnp-5883	606	80	,	,	PUNCT
fnp-5883	606	81	m.c	m.c	PROPN
fnp-5883	606	82	.	.	PROPN
fnp-5883	606	83	,	,	PUNCT
fnp-5883	606	84	naismith	naismith	PROPN
fnp-5883	606	85	,	,	PUNCT
fnp-5883	606	86	j.h	j.h	PROPN
fnp-5883	606	87	.	.	PROPN
fnp-5883	606	88	,	,	PUNCT
fnp-5883	606	89	grange	grange	PROPN
fnp-5883	606	90	,	,	PUNCT
fnp-5883	606	91	m.	m.	NOUN
fnp-5883	606	92	,	,	PUNCT
fnp-5883	606	93	2023	2023	NUM
fnp-5883	606	94	.	.	PUNCT
fnp-5883	607	1	cryo	cryo	NOUN
fnp-5883	607	2	-	-	PUNCT
fnp-5883	607	3	plasma	plasma	NOUN
fnp-5883	607	4	fib	fib	ADJ
fnp-5883	607	5	/	/	SYM
fnp-5883	607	6	sem	sem	NOUN
fnp-5883	607	7	volume	volume	NOUN
fnp-5883	607	8	imaging	imaging	NOUN
fnp-5883	607	9	of	of	ADP
fnp-5883	607	10	biological	biological	ADJ
fnp-5883	607	11	specimens	specimen	NOUN
fnp-5883	607	12	.	.	PUNCT
fnp-5883	608	1	elife	elife	NOUN
fnp-5883	608	2	12	12	NUM
fnp-5883	608	3	,	,	PUNCT
fnp-5883	608	4	e83623	e83623	NOUN
fnp-5883	608	5	.	.	PUNCT
fnp-5883	609	1	https://doi.org/10.7554/elife.83623	https://doi.org/10.7554/elife.83623	ADJ
fnp-5883	609	2	durrenberger	durrenberger	NOUN
fnp-5883	609	3	,	,	PUNCT
fnp-5883	609	4	p.f	p.f	PROPN
fnp-5883	609	5	.	.	PROPN
fnp-5883	609	6	,	,	PUNCT
fnp-5883	609	7	fernando	fernando	PROPN
fnp-5883	609	8	,	,	PUNCT
fnp-5883	609	9	s.	s.	PROPN
fnp-5883	609	10	,	,	PUNCT
fnp-5883	609	11	kashefi	kashefi	PROPN
fnp-5883	609	12	,	,	PUNCT
fnp-5883	609	13	s.n	s.n	PROPN
fnp-5883	609	14	.	.	PROPN
fnp-5883	609	15	,	,	PUNCT
fnp-5883	609	16	ferrer	ferrer	PROPN
fnp-5883	609	17	,	,	PUNCT
fnp-5883	609	18	i.	i.	PROPN
fnp-5883	609	19	,	,	PUNCT
fnp-5883	609	20	hauw	hauw	PROPN
fnp-5883	609	21	,	,	PUNCT
fnp-5883	609	22	j.-j	j.-j	PROPN
fnp-5883	609	23	.	.	PROPN
fnp-5883	609	24	,	,	PUNCT
fnp-5883	609	25	seilhean	seilhean	VERB
fnp-5883	609	26	,	,	PUNCT
fnp-5883	609	27	d.	d.	PROPN
fnp-5883	609	28	,	,	PUNCT
fnp-5883	609	29	smith	smith	PROPN
fnp-5883	609	30	,	,	PUNCT
fnp-5883	609	31	c.	c.	PROPN
fnp-5883	609	32	,	,	PUNCT
fnp-5883	609	33	walker	walker	PROPN
fnp-5883	609	34	,	,	PUNCT
fnp-5883	609	35	r.	r.	PROPN
fnp-5883	609	36	,	,	PUNCT
fnp-5883	609	37	al	al	PROPN
fnp-5883	609	38	-	-	PUNCT
fnp-5883	609	39	sarraj	sarraj	VERB
fnp-5883	609	40	,	,	PUNCT
fnp-5883	609	41	s.	s.	PROPN
fnp-5883	609	42	,	,	PUNCT
fnp-5883	609	43	troakes	troake	NOUN
fnp-5883	609	44	,	,	PUNCT
fnp-5883	609	45	c.	c.	NOUN
fnp-5883	609	46	,	,	PUNCT
fnp-5883	609	47	palkovits	palkovit	NOUN
fnp-5883	609	48	,	,	PUNCT
fnp-5883	609	49	m.	m.	NOUN
fnp-5883	609	50	,	,	PUNCT
fnp-5883	609	51	kasztner	kasztner	NOUN
fnp-5883	609	52	,	,	PUNCT
fnp-5883	609	53	m.	m.	NOUN
fnp-5883	609	54	,	,	PUNCT
fnp-5883	609	55	huitinga	huitinga	PROPN
fnp-5883	609	56	,	,	PUNCT
fnp-5883	609	57	i.	i.	NOUN
fnp-5883	609	58	,	,	PUNCT
fnp-5883	609	59	arzberger	arzberger	NOUN
fnp-5883	609	60	,	,	PUNCT
fnp-5883	609	61	t.	t.	PROPN
fnp-5883	609	62	,	,	PUNCT
fnp-5883	609	63	dexter	dexter	PROPN
fnp-5883	609	64	,	,	PUNCT
fnp-5883	609	65	d.t	d.t	PROPN
fnp-5883	609	66	.	.	PROPN
fnp-5883	609	67	,	,	PUNCT
fnp-5883	609	68	kretzschmar	kretzschmar	PROPN
fnp-5883	609	69	,	,	PUNCT
fnp-5883	609	70	h.	h.	PROPN
fnp-5883	609	71	,	,	PUNCT
fnp-5883	609	72	reynolds	reynolds	PROPN
fnp-5883	609	73	,	,	PUNCT
fnp-5883	609	74	r.	r.	PROPN
fnp-5883	609	75	,	,	PUNCT
fnp-5883	609	76	2010	2010	NUM
fnp-5883	609	77	.	.	PUNCT
fnp-5883	610	1	effects	effect	NOUN
fnp-5883	610	2	of	of	ADP
fnp-5883	610	3	antemortem	antemortem	NOUN
fnp-5883	610	4	and	and	CCONJ
fnp-5883	610	5	postmortem	postmortem	NOUN
fnp-5883	610	6	variables	variable	NOUN
fnp-5883	610	7	on	on	ADP
fnp-5883	610	8	human	human	ADJ
fnp-5883	610	9	brain	brain	NOUN
fnp-5883	610	10	mrna	mrna	PROPN
fnp-5883	610	11	quality	quality	PROPN
fnp-5883	610	12	:	:	PUNCT
fnp-5883	610	13	a	a	DET
fnp-5883	610	14	brainnet	brainnet	NOUN
fnp-5883	610	15	europe	europe	PROPN
fnp-5883	610	16	study	study	PROPN
fnp-5883	610	17	.	.	PUNCT
fnp-5883	611	1	j.	j.	PROPN
fnp-5883	611	2	neuropathol	neuropathol	PROPN
fnp-5883	611	3	.	.	PUNCT
fnp-5883	612	1	exp	exp	PROPN
fnp-5883	612	2	.	.	PUNCT
fnp-5883	613	1	neurol	neurol	PROPN
fnp-5883	613	2	.	.	PUNCT
fnp-5883	614	1	69	69	NUM
fnp-5883	614	2	,	,	PUNCT
fnp-5883	614	3	70–81	70–81	NUM
fnp-5883	614	4	.	.	PUNCT
fnp-5883	615	1	https://doi.org/10.1097/nen.0b013e3181c7e32f	https://doi.org/10.1097/nen.0b013e3181c7e32f	ADJ
fnp-5883	615	2	estrada	estrada	PROPN
fnp-5883	615	3	,	,	PUNCT
fnp-5883	615	4	l.i	l.i	PROPN
fnp-5883	615	5	.	.	PROPN
fnp-5883	615	6	,	,	PUNCT
fnp-5883	615	7	robinson	robinson	PROPN
fnp-5883	615	8	,	,	PUNCT
fnp-5883	615	9	a.a	a.a	PROPN
fnp-5883	615	10	.	.	PROPN
fnp-5883	615	11	,	,	PUNCT
fnp-5883	615	12	amaral	amaral	PROPN
fnp-5883	615	13	,	,	PUNCT
fnp-5883	615	14	a.c	a.c	PROPN
fnp-5883	615	15	.	.	PROPN
fnp-5883	615	16	,	,	PUNCT
fnp-5883	615	17	giannaris	giannaris	PROPN
fnp-5883	615	18	,	,	PUNCT
fnp-5883	615	19	e.l	e.l	PROPN
fnp-5883	615	20	.	.	PROPN
fnp-5883	615	21	,	,	PUNCT
fnp-5883	615	22	heyworth	heyworth	PROPN
fnp-5883	615	23	,	,	PUNCT
fnp-5883	615	24	n.c	n.c	PROPN
fnp-5883	615	25	.	.	PROPN
fnp-5883	615	26	,	,	PUNCT
fnp-5883	615	27	mortazavi	mortazavi	PROPN
fnp-5883	615	28	,	,	PUNCT
fnp-5883	615	29	f.	f.	PROPN
fnp-5883	615	30	,	,	PUNCT
fnp-5883	615	31	ngwenya	ngwenya	PROPN
fnp-5883	615	32	,	,	PUNCT
fnp-5883	615	33	l.b	l.b	PROPN
fnp-5883	615	34	.	.	PROPN
fnp-5883	615	35	,	,	PUNCT
fnp-5883	615	36	roberts	roberts	PROPN
fnp-5883	615	37	,	,	PUNCT
fnp-5883	615	38	d.e	d.e	PROPN
fnp-5883	615	39	.	.	PROPN
fnp-5883	615	40	,	,	PUNCT
fnp-5883	615	41	cabral	cabral	PROPN
fnp-5883	615	42	,	,	PUNCT
fnp-5883	615	43	h.j	h.j	PROPN
fnp-5883	615	44	.	.	PROPN
fnp-5883	615	45	,	,	PUNCT
fnp-5883	615	46	killiany	killiany	PROPN
fnp-5883	615	47	,	,	PUNCT
fnp-5883	615	48	r.j	r.j	PROPN
fnp-5883	615	49	.	.	PROPN
fnp-5883	615	50	,	,	PUNCT
fnp-5883	615	51	rosene	rosene	NOUN
fnp-5883	615	52	,	,	PUNCT
fnp-5883	615	53	d.l	d.l	PROPN
fnp-5883	615	54	.	.	PROPN
fnp-5883	615	55	,	,	PUNCT
fnp-5883	615	56	2017	2017	NUM
fnp-5883	615	57	.	.	PUNCT
fnp-5883	616	1	evaluation	evaluation	NOUN
fnp-5883	616	2	of	of	ADP
fnp-5883	616	3	long	long	ADJ
fnp-5883	616	4	-	-	PUNCT
fnp-5883	616	5	term	term	NOUN
fnp-5883	616	6	cryostorage	cryostorage	NOUN
fnp-5883	616	7	of	of	ADP
fnp-5883	616	8	brain	brain	NOUN
fnp-5883	616	9	tissue	tissue	NOUN
fnp-5883	616	10	sections	section	NOUN
fnp-5883	616	11	for	for	ADP
fnp-5883	616	12	quantitative	quantitative	ADJ
fnp-5883	616	13	histochemistry	histochemistry	NOUN
fnp-5883	616	14	.	.	PUNCT
fnp-5883	617	1	j.	j.	PROPN
fnp-5883	617	2	histochem	histochem	PROPN
fnp-5883	617	3	.	.	PUNCT
fnp-5883	618	1	cytochem	cytochem	PROPN
fnp-5883	618	2	.	.	PUNCT
fnp-5883	619	1	65	65	NUM
fnp-5883	619	2	,	,	PUNCT
fnp-5883	619	3	153–171	153–171	NUM
fnp-5883	619	4	.	.	PUNCT
fnp-5883	620	1	https://doi.org/10.1369/0022155416686934	https://doi.org/10.1369/0022155416686934	PRON
fnp-5883	620	2	fahy	fahy	PROPN
fnp-5883	620	3	,	,	PUNCT
fnp-5883	620	4	g.m	g.m	PROPN
fnp-5883	620	5	.	.	PROPN
fnp-5883	620	6	,	,	PUNCT
fnp-5883	620	7	wowk	wowk	PROPN
fnp-5883	620	8	,	,	PUNCT
fnp-5883	620	9	b.	b.	PROPN
fnp-5883	620	10	,	,	PUNCT
fnp-5883	620	11	2021	2021	NUM
fnp-5883	620	12	.	.	PUNCT
fnp-5883	621	1	principles	principle	NOUN
fnp-5883	621	2	of	of	ADP
fnp-5883	621	3	ice	ice	NOUN
fnp-5883	621	4	-	-	PUNCT
fnp-5883	621	5	free	free	ADJ
fnp-5883	621	6	cryopreservation	cryopreservation	NOUN
fnp-5883	621	7	by	by	ADP
fnp-5883	621	8	vitrification	vitrification	NOUN
fnp-5883	621	9	.	.	PUNCT
fnp-5883	622	1	methods	method	NOUN
fnp-5883	622	2	mol	mol	PROPN
fnp-5883	622	3	.	.	PUNCT
fnp-5883	623	1	biol	biol	PROPN
fnp-5883	623	2	.	.	PUNCT
fnp-5883	624	1	clifton	clifton	PROPN
fnp-5883	624	2	nj	nj	PROPN
fnp-5883	624	3	2180	2180	NUM
fnp-5883	624	4	,	,	PUNCT
fnp-5883	624	5	27–97	27–97	NUM
fnp-5883	624	6	.	.	PUNCT
fnp-5883	625	1	https://doi.org/10.1007/978-1-0716-0783-1_2	https://doi.org/10.1007/978-1-0716-0783-1_2	PROPN
fnp-5883	625	2	fahy	fahy	PROPN
fnp-5883	625	3	,	,	PUNCT
fnp-5883	625	4	g.m	g.m	PROPN
fnp-5883	625	5	.	.	PROPN
fnp-5883	625	6	,	,	PUNCT
fnp-5883	625	7	wowk	wowk	PROPN
fnp-5883	625	8	,	,	PUNCT
fnp-5883	625	9	b.	b.	PROPN
fnp-5883	625	10	,	,	PUNCT
fnp-5883	625	11	2015	2015	NUM
fnp-5883	625	12	.	.	PUNCT
fnp-5883	626	1	principles	principle	NOUN
fnp-5883	626	2	of	of	ADP
fnp-5883	626	3	cryopreservation	cryopreservation	NOUN
fnp-5883	626	4	by	by	ADP
fnp-5883	626	5	vitrification	vitrification	NOUN
fnp-5883	626	6	.	.	PUNCT
fnp-5883	627	1	methods	method	NOUN
fnp-5883	627	2	mol	mol	PROPN
fnp-5883	627	3	.	.	PUNCT
fnp-5883	628	1	biol	biol	PROPN
fnp-5883	628	2	.	.	PUNCT
fnp-5883	629	1	clifton	clifton	PROPN
fnp-5883	629	2	nj	nj	PROPN
fnp-5883	629	3	1257	1257	NUM
fnp-5883	629	4	,	,	PUNCT
fnp-5883	629	5	21–82	21–82	NUM
fnp-5883	629	6	.	.	PUNCT
fnp-5883	630	1	https://doi.org/10.1007/978-1-4939-2193-5_2	https://doi.org/10.1007/978-1-4939-2193-5_2	PROPN
fnp-5883	630	2	fortunato	fortunato	PROPN
fnp-5883	630	3	,	,	PUNCT
fnp-5883	630	4	f.	f.	PROPN
fnp-5883	630	5	,	,	PUNCT
fnp-5883	630	6	hackert	hackert	PROPN
fnp-5883	630	7	,	,	PUNCT
fnp-5883	630	8	t.	t.	NOUN
fnp-5883	630	9	,	,	PUNCT
fnp-5883	630	10	büchler	büchler	ADJ
fnp-5883	630	11	,	,	PUNCT
fnp-5883	630	12	m.w	m.w	PROPN
fnp-5883	630	13	.	.	PROPN
fnp-5883	630	14	,	,	PUNCT
fnp-5883	630	15	kroemer	kroemer	PROPN
fnp-5883	630	16	,	,	PUNCT
fnp-5883	630	17	g.	g.	PROPN
fnp-5883	630	18	,	,	PUNCT
fnp-5883	630	19	2016	2016	NUM
fnp-5883	630	20	.	.	PUNCT
fnp-5883	631	1	retrospective	retrospective	ADJ
fnp-5883	631	2	electron	electron	NOUN
fnp-5883	631	3	microscopy	microscopy	NOUN
fnp-5883	631	4	:	:	PUNCT
fnp-5883	631	5	preservation	preservation	NOUN
fnp-5883	631	6	of	of	ADP
fnp-5883	631	7	fine	fine	ADJ
fnp-5883	631	8	structure	structure	NOUN
fnp-5883	631	9	by	by	ADP
fnp-5883	631	10	freezing	freeze	VERB
fnp-5883	631	11	and	and	CCONJ
fnp-5883	631	12	aldehyde	aldehyde	NOUN
fnp-5883	631	13	fixation	fixation	NOUN
fnp-5883	631	14	.	.	PUNCT
fnp-5883	632	1	mol	mol	PROPN
fnp-5883	632	2	.	.	PROPN
fnp-5883	632	3	cell	cell	NOUN
fnp-5883	632	4	.	.	PUNCT
fnp-5883	633	1	oncol	oncol	ADJ
fnp-5883	633	2	.	.	PUNCT
fnp-5883	634	1	3	3	NUM
fnp-5883	634	2	,	,	PUNCT
fnp-5883	634	3	e1251382	e1251382	PROPN
fnp-5883	634	4	.	.	PUNCT
fnp-5883	635	1	https://doi.org/10.1080/23723556.2016.1251382	https://doi.org/10.1080/23723556.2016.1251382	PROPN
fnp-5883	635	2	galhuber	galhuber	PROPN
fnp-5883	635	3	,	,	PUNCT
fnp-5883	635	4	m.	m.	NOUN
fnp-5883	635	5	,	,	PUNCT
fnp-5883	635	6	kupper	kupper	PROPN
fnp-5883	635	7	,	,	PUNCT
fnp-5883	635	8	n.	n.	NOUN
fnp-5883	635	9	,	,	PUNCT
fnp-5883	635	10	dohr	dohr	PROPN
fnp-5883	635	11	,	,	PUNCT
fnp-5883	635	12	g.	g.	PROPN
fnp-5883	635	13	,	,	PUNCT
fnp-5883	635	14	gauster	gauster	NOUN
fnp-5883	635	15	,	,	PUNCT
fnp-5883	635	16	m.	m.	NOUN
fnp-5883	635	17	,	,	PUNCT
fnp-5883	635	18	kwapiszewska	kwapiszewska	PROPN
fnp-5883	635	19	,	,	PUNCT
fnp-5883	635	20	g.	g.	PROPN
fnp-5883	635	21	,	,	PUNCT
fnp-5883	635	22	olschewski	olschewski	PROPN
fnp-5883	635	23	,	,	PUNCT
fnp-5883	635	24	a.	a.	NOUN
fnp-5883	635	25	,	,	PUNCT
fnp-5883	635	26	jandl	jandl	PROPN
fnp-5883	635	27	,	,	PUNCT
fnp-5883	635	28	k.	k.	PROPN
fnp-5883	635	29	,	,	PUNCT
fnp-5883	635	30	gschwandtner	gschwandtner	NOUN
fnp-5883	635	31	,	,	PUNCT
fnp-5883	635	32	e.	e.	PROPN
fnp-5883	635	33	,	,	PUNCT
fnp-5883	635	34	schweiger	schweiger	PROPN
fnp-5883	635	35	,	,	PUNCT
fnp-5883	635	36	m.	m.	NOUN
fnp-5883	635	37	,	,	PUNCT
fnp-5883	635	38	kratky	kratky	PROPN
fnp-5883	635	39	,	,	PUNCT
fnp-5883	635	40	d.	d.	PROPN
fnp-5883	635	41	,	,	PUNCT
fnp-5883	635	42	leitinger	leitinger	PROPN
fnp-5883	635	43	,	,	PUNCT
fnp-5883	635	44	g.	g.	PROPN
fnp-5883	635	45	,	,	PUNCT
fnp-5883	635	46	prokesch	prokesch	PROPN
fnp-5883	635	47	,	,	PUNCT
fnp-5883	635	48	a.	a.	PROPN
fnp-5883	635	49	,	,	PUNCT
fnp-5883	635	50	kolb	kolb	PROPN
fnp-5883	635	51	,	,	PUNCT
fnp-5883	635	52	d.	d.	PROPN
fnp-5883	635	53	,	,	PUNCT
fnp-5883	635	54	2021	2021	NUM
fnp-5883	635	55	.	.	PUNCT
fnp-5883	636	1	simple	simple	ADJ
fnp-5883	636	2	method	method	NOUN
fnp-5883	636	3	of	of	ADP
fnp-5883	636	4	thawing	thaw	VERB
fnp-5883	636	5	cryo	cryo	NOUN
fnp-5883	636	6	-	-	PUNCT
fnp-5883	636	7	stored	store	VERB
fnp-5883	636	8	samples	sample	NOUN
fnp-5883	636	9	preserves	preserve	VERB
fnp-5883	636	10	ultrastructural	ultrastructural	ADJ
fnp-5883	636	11	features	feature	NOUN
fnp-5883	636	12	in	in	ADP
fnp-5883	636	13	electron	electron	NOUN
fnp-5883	636	14	microscopy	microscopy	NOUN
fnp-5883	636	15	.	.	PUNCT
fnp-5883	637	1	histochem	histochem	PROPN
fnp-5883	637	2	.	.	PUNCT
fnp-5883	638	1	cell	cell	PROPN
fnp-5883	638	2	biol	biol	PROPN
fnp-5883	638	3	.	.	PUNCT
fnp-5883	639	1	155	155	NUM
fnp-5883	639	2	,	,	PUNCT
fnp-5883	639	3	593–603	593–603	NUM
fnp-5883	639	4	.	.	PUNCT
fnp-5883	640	1	https://doi.org/10.1007/s00418-020-01952-z	https://doi.org/10.1007/s00418-020-01952-z	NOUN
fnp-5883	640	2	gasperoni	gasperoni	NOUN
fnp-5883	640	3	,	,	PUNCT
fnp-5883	640	4	j.	j.	PROPN
fnp-5883	640	5	,	,	PUNCT
fnp-5883	640	6	dworkin	dworkin	PROPN
fnp-5883	640	7	,	,	PUNCT
fnp-5883	640	8	s.	s.	PROPN
fnp-5883	640	9	,	,	PUNCT
fnp-5883	640	10	2024	2024	NUM
fnp-5883	640	11	.	.	PUNCT
fnp-5883	641	1	cryosectioning	cryosectioning	NOUN
fnp-5883	641	2	and	and	CCONJ
fnp-5883	641	3	immunohistochemistry	immunohistochemistry	NOUN
fnp-5883	641	4	using	use	VERB
fnp-5883	641	5	frozen	frozen	ADJ
fnp-5883	641	6	adult	adult	NOUN
fnp-5883	641	7	murine	murine	ADJ
fnp-5883	641	8	brain	brain	NOUN
fnp-5883	641	9	neural	neural	ADJ
fnp-5883	641	10	tissue	tissue	NOUN
fnp-5883	641	11	.	.	PUNCT
fnp-5883	642	1	methods	method	NOUN
fnp-5883	642	2	mol	mol	PROPN
fnp-5883	642	3	.	.	PUNCT
fnp-5883	643	1	biol	biol	PROPN
fnp-5883	643	2	.	.	PUNCT
fnp-5883	644	1	clifton	clifton	PROPN
fnp-5883	644	2	nj	nj	PROPN
fnp-5883	644	3	2746	2746	NUM
fnp-5883	644	4	,	,	PUNCT
fnp-5883	644	5	155–163	155–163	NUM
fnp-5883	644	6	.	.	PUNCT
fnp-5883	645	1	https://doi.org/10.1007/978-1-0716-3585-8_13	https://doi.org/10.1007/978-1-0716-3585-8_13	PROPN
fnp-5883	645	2	gellhaar	gellhaar	PROPN
fnp-5883	645	3	,	,	PUNCT
fnp-5883	645	4	s.	s.	PROPN
fnp-5883	645	5	,	,	PUNCT
fnp-5883	645	6	sunnemark	sunnemark	PROPN
fnp-5883	645	7	,	,	PUNCT
fnp-5883	645	8	d.	d.	PROPN
fnp-5883	645	9	,	,	PUNCT
fnp-5883	645	10	eriksson	eriksson	PROPN
fnp-5883	645	11	,	,	PUNCT
fnp-5883	645	12	h.	h.	PROPN
fnp-5883	645	13	,	,	PUNCT
fnp-5883	645	14	olson	olson	PROPN
fnp-5883	645	15	,	,	PUNCT
fnp-5883	645	16	l.	l.	PROPN
fnp-5883	645	17	,	,	PUNCT
fnp-5883	645	18	galter	galter	NOUN
fnp-5883	645	19	,	,	PUNCT
fnp-5883	645	20	d.	d.	PROPN
fnp-5883	645	21	,	,	PUNCT
fnp-5883	645	22	2017	2017	NUM
fnp-5883	645	23	.	.	PUNCT
fnp-5883	646	1	myeloperoxidase	myeloperoxidase	NOUN
fnp-5883	646	2	-	-	PUNCT
fnp-5883	646	3	immunoreactive	immunoreactive	ADJ
fnp-5883	646	4	cells	cell	NOUN
fnp-5883	646	5	are	be	AUX
fnp-5883	646	6	significantly	significantly	ADV
fnp-5883	646	7	increased	increase	VERB
fnp-5883	646	8	in	in	ADP
fnp-5883	646	9	brain	brain	NOUN
fnp-5883	646	10	areas	area	NOUN
fnp-5883	646	11	affected	affect	VERB
fnp-5883	646	12	by	by	ADP
fnp-5883	646	13	neurodegeneration	neurodegeneration	NOUN
fnp-5883	646	14	in	in	ADP
fnp-5883	646	15	parkinson	parkinson	NOUN
fnp-5883	646	16	’s	’s	PART
fnp-5883	646	17	and	and	CCONJ
fnp-5883	646	18	alzheimer	alzheimer	PROPN
fnp-5883	646	19	’s	’s	PART
fnp-5883	646	20	disease	disease	NOUN
fnp-5883	646	21	.	.	PUNCT
fnp-5883	647	1	cell	cell	NOUN
fnp-5883	647	2	tissue	tissue	NOUN
fnp-5883	647	3	res	re	NOUN
fnp-5883	647	4	.	.	PUNCT
fnp-5883	647	5	369	369	NUM
fnp-5883	647	6	,	,	PUNCT
fnp-5883	647	7	445–454	445–454	NUM
fnp-5883	647	8	.	.	PUNCT
fnp-5883	648	1	https://doi.org/10.1007/s00441-017-2626-8	https://doi.org/10.1007/s00441-017-2626-8	PROPN
fnp-5883	648	2	gerber	gerber	PROPN
fnp-5883	648	3	,	,	PUNCT
fnp-5883	648	4	d.	d.	PROPN
fnp-5883	648	5	,	,	PUNCT
fnp-5883	648	6	wilen	wilen	PROPN
fnp-5883	648	7	,	,	PUNCT
fnp-5883	648	8	l.a	l.a	PROPN
fnp-5883	648	9	.	.	PROPN
fnp-5883	648	10	,	,	PUNCT
fnp-5883	648	11	poydenot	poydenot	ADV
fnp-5883	648	12	,	,	PUNCT
fnp-5883	648	13	f.	f.	PROPN
fnp-5883	648	14	,	,	PUNCT
fnp-5883	648	15	dufresne	dufresne	PROPN
fnp-5883	648	16	,	,	PUNCT
fnp-5883	648	17	e.r	e.r	PROPN
fnp-5883	648	18	.	.	PROPN
fnp-5883	648	19	,	,	PUNCT
fnp-5883	648	20	style	style	NOUN
fnp-5883	648	21	,	,	PUNCT
fnp-5883	648	22	r.w	r.w	PROPN
fnp-5883	648	23	.	.	PROPN
fnp-5883	648	24	,	,	PUNCT
fnp-5883	648	25	2022	2022	NUM
fnp-5883	648	26	.	.	PUNCT
fnp-5883	649	1	stress	stress	NOUN
fnp-5883	649	2	accumulation	accumulation	NOUN
fnp-5883	649	3	by	by	ADP
fnp-5883	649	4	confined	confine	VERB
fnp-5883	649	5	ice	ice	NOUN
fnp-5883	649	6	in	in	ADP
fnp-5883	649	7	a	a	DET
fnp-5883	649	8	temperature	temperature	NOUN
fnp-5883	649	9	gradient	gradient	NOUN
fnp-5883	649	10	.	.	PUNCT
fnp-5883	650	1	proc	proc	PROPN
fnp-5883	650	2	.	.	PUNCT
fnp-5883	651	1	natl	natl	PROPN
fnp-5883	651	2	.	.	PUNCT
fnp-5883	652	1	acad	acad	PROPN
fnp-5883	652	2	.	.	PUNCT
fnp-5883	653	1	sci	sci	PROPN
fnp-5883	653	2	.	.	PUNCT
fnp-5883	653	3	u.	u.	PROPN
fnp-5883	653	4	s.	s.	PROPN
fnp-5883	653	5	a.	a.	PROPN
fnp-5883	653	6	119	119	NUM
fnp-5883	653	7	,	,	PUNCT
fnp-5883	653	8	e2200748119	e2200748119	PROPN
fnp-5883	653	9	.	.	PUNCT
fnp-5883	654	1	https://doi.org/10.1073/pnas.2200748119	https://doi.org/10.1073/pnas.2200748119	PROPN
fnp-5883	654	2	german	german	PROPN
fnp-5883	654	3	,	,	PUNCT
fnp-5883	654	4	a.	a.	PROPN
fnp-5883	654	5	,	,	PUNCT
fnp-5883	654	6	akdaş	akdaş	PROPN
fnp-5883	654	7	,	,	PUNCT
fnp-5883	654	8	e.y	e.y	PROPN
fnp-5883	654	9	.	.	PROPN
fnp-5883	654	10	,	,	PUNCT
fnp-5883	654	11	2024	2024	NUM
fnp-5883	654	12	.	.	PUNCT
fnp-5883	655	1	excitability	excitability	NOUN
fnp-5883	655	2	and	and	CCONJ
fnp-5883	655	3	synaptic	synaptic	ADJ
fnp-5883	655	4	transmission	transmission	NOUN
fnp-5883	655	5	after	after	ADP
fnp-5883	655	6	vitrification	vitrification	NOUN
fnp-5883	655	7	of	of	ADP
fnp-5883	655	8	mouse	mouse	NOUN
fnp-5883	655	9	corticohippocampal	corticohippocampal	ADJ
fnp-5883	655	10	slices	slice	NOUN
fnp-5883	655	11	.	.	PUNCT
fnp-5883	656	1	https://doi.org/10.1101/2024.06.03.597218	https://doi.org/10.1101/2024.06.03.597218	ADJ
fnp-5883	656	2	gilkey	gilkey	NOUN
fnp-5883	656	3	,	,	PUNCT
fnp-5883	656	4	j.c	j.c	PROPN
fnp-5883	656	5	.	.	PROPN
fnp-5883	656	6	,	,	PUNCT
fnp-5883	656	7	staehelin	staehelin	PROPN
fnp-5883	656	8	,	,	PUNCT
fnp-5883	656	9	l.a	l.a	PROPN
fnp-5883	656	10	.	.	PROPN
fnp-5883	656	11	,	,	PUNCT
fnp-5883	656	12	1986	1986	NUM
fnp-5883	656	13	.	.	PUNCT
fnp-5883	657	1	advances	advance	NOUN
fnp-5883	657	2	in	in	ADP
fnp-5883	657	3	ultrarapid	ultrarapid	NOUN
fnp-5883	657	4	freezing	freezing	NOUN
fnp-5883	657	5	for	for	ADP
fnp-5883	657	6	the	the	DET
fnp-5883	657	7	preservation	preservation	NOUN
fnp-5883	657	8	of	of	ADP
fnp-5883	657	9	cellular	cellular	ADJ
fnp-5883	657	10	ultrastructure	ultrastructure	NOUN
fnp-5883	657	11	.	.	PUNCT
fnp-5883	658	1	j.	j.	PROPN
fnp-5883	658	2	electron	electron	PROPN
fnp-5883	658	3	microsc	microsc	PROPN
fnp-5883	658	4	.	.	PUNCT
fnp-5883	659	1	tech	tech	NOUN
fnp-5883	659	2	.	.	PUNCT
fnp-5883	660	1	3	3	NUM
fnp-5883	660	2	,	,	PUNCT
fnp-5883	660	3	177–210	177–210	NUM
fnp-5883	660	4	.	.	PUNCT
fnp-5883	661	1	https://doi.org/10.1002/jemt.1060030206	https://doi.org/10.1002/jemt.1060030206	PROPN
fnp-5883	661	2	giwa	giwa	PROPN
fnp-5883	661	3	,	,	PUNCT
fnp-5883	661	4	s.	s.	PROPN
fnp-5883	661	5	,	,	PUNCT
fnp-5883	661	6	lewis	lewis	PROPN
fnp-5883	661	7	,	,	PUNCT
fnp-5883	661	8	j.k	j.k	PROPN
fnp-5883	661	9	.	.	PROPN
fnp-5883	661	10	,	,	PUNCT
fnp-5883	661	11	alvarez	alvarez	PROPN
fnp-5883	661	12	,	,	PUNCT
fnp-5883	661	13	l.	l.	PROPN
fnp-5883	661	14	,	,	PUNCT
fnp-5883	661	15	langer	langer	PROPN
fnp-5883	661	16	,	,	PUNCT
fnp-5883	661	17	r.	r.	PROPN
fnp-5883	661	18	,	,	PUNCT
fnp-5883	661	19	roth	roth	PROPN
fnp-5883	661	20	,	,	PUNCT
fnp-5883	661	21	a.e	a.e	PROPN
fnp-5883	661	22	.	.	PROPN
fnp-5883	661	23	,	,	PUNCT
fnp-5883	661	24	church	church	PROPN
fnp-5883	661	25	,	,	PUNCT
fnp-5883	661	26	g.m	g.m	PROPN
fnp-5883	661	27	.	.	PROPN
fnp-5883	661	28	,	,	PUNCT
fnp-5883	661	29	markmann	markmann	PROPN
fnp-5883	661	30	,	,	PUNCT
fnp-5883	661	31	j.f	j.f	PROPN
fnp-5883	661	32	.	.	PROPN
fnp-5883	661	33	,	,	PUNCT
fnp-5883	661	34	sachs	sachs	PROPN
fnp-5883	661	35	,	,	PUNCT
fnp-5883	661	36	d.h	d.h	PROPN
fnp-5883	661	37	.	.	PROPN
fnp-5883	661	38	,	,	PUNCT
fnp-5883	661	39	chandraker	chandraker	PROPN
fnp-5883	661	40	,	,	PUNCT
fnp-5883	661	41	a.	a.	PROPN
fnp-5883	661	42	,	,	PUNCT
fnp-5883	661	43	wertheim	wertheim	PROPN
fnp-5883	661	44	,	,	PUNCT
fnp-5883	661	45	j.a	j.a	PROPN
fnp-5883	661	46	.	.	PROPN
fnp-5883	661	47	,	,	PUNCT
fnp-5883	661	48	rothblatt	rothblatt	NOUN
fnp-5883	661	49	,	,	PUNCT
fnp-5883	661	50	m.	m.	NOUN
fnp-5883	661	51	,	,	PUNCT
fnp-5883	661	52	boyden	boyden	PROPN
fnp-5883	661	53	,	,	PUNCT
fnp-5883	661	54	e.s	e.s	PROPN
fnp-5883	661	55	.	.	PROPN
fnp-5883	661	56	,	,	PUNCT
fnp-5883	661	57	eidbo	eidbo	PROPN
fnp-5883	661	58	,	,	PUNCT
fnp-5883	661	59	e.	e.	PROPN
fnp-5883	661	60	,	,	PUNCT
fnp-5883	661	61	lee	lee	PROPN
fnp-5883	661	62	,	,	PUNCT
fnp-5883	661	63	w.p.a	w.p.a	NOUN
fnp-5883	661	64	.	.	PUNCT
fnp-5883	661	65	,	,	PUNCT
fnp-5883	661	66	pomahac	pomahac	PROPN
fnp-5883	661	67	,	,	PUNCT
fnp-5883	661	68	b.	b.	PROPN
fnp-5883	661	69	,	,	PUNCT
fnp-5883	661	70	brandacher	brandacher	ADV
fnp-5883	661	71	,	,	PUNCT
fnp-5883	661	72	g.	g.	PROPN
fnp-5883	661	73	,	,	PUNCT
fnp-5883	661	74	weinstock	weinstock	NOUN
fnp-5883	661	75	,	,	PUNCT
fnp-5883	661	76	d.m	d.m	PROPN
fnp-5883	661	77	.	.	PROPN
fnp-5883	661	78	,	,	PUNCT
fnp-5883	661	79	elliott	elliott	PROPN
fnp-5883	661	80	,	,	PUNCT
fnp-5883	661	81	g.	g.	PROPN
fnp-5883	661	82	,	,	PUNCT
fnp-5883	661	83	nelson	nelson	PROPN
fnp-5883	661	84	,	,	PUNCT
fnp-5883	661	85	d.	d.	PROPN
fnp-5883	661	86	,	,	PUNCT
fnp-5883	661	87	acker	acker	PROPN
fnp-5883	661	88	,	,	PUNCT
fnp-5883	661	89	j.p	j.p	PROPN
fnp-5883	661	90	.	.	PROPN
fnp-5883	661	91	,	,	PUNCT
fnp-5883	661	92	uygun	uygun	PROPN
fnp-5883	661	93	,	,	PUNCT
fnp-5883	661	94	k.	k.	PROPN
fnp-5883	661	95	,	,	PUNCT
fnp-5883	661	96	schmalz	schmalz	PROPN
fnp-5883	661	97	,	,	PUNCT
fnp-5883	661	98	b.	b.	PROPN
fnp-5883	661	99	,	,	PUNCT
fnp-5883	661	100	weegman	weegman	NOUN
fnp-5883	661	101	,	,	PUNCT
fnp-5883	661	102	b.p	b.p	PROPN
fnp-5883	661	103	.	.	PROPN
fnp-5883	661	104	,	,	PUNCT
fnp-5883	661	105	tocchio	tocchio	PROPN
fnp-5883	661	106	,	,	PUNCT
fnp-5883	661	107	a.	a.	PROPN
fnp-5883	661	108	,	,	PUNCT
fnp-5883	661	109	fahy	fahy	PROPN
fnp-5883	661	110	,	,	PUNCT
fnp-5883	661	111	g.m	g.m	PROPN
fnp-5883	661	112	.	.	PROPN
fnp-5883	661	113	,	,	PUNCT
fnp-5883	661	114	storey	storey	PROPN
fnp-5883	661	115	,	,	PUNCT
fnp-5883	661	116	k.b	k.b	PROPN
fnp-5883	661	117	.	.	PROPN
fnp-5883	661	118	,	,	PUNCT
fnp-5883	661	119	rubinsky	rubinsky	NOUN
fnp-5883	661	120	,	,	PUNCT
fnp-5883	661	121	b.	b.	PROPN
fnp-5883	661	122	,	,	PUNCT
fnp-5883	661	123	bischof	bischof	NOUN
fnp-5883	661	124	,	,	PUNCT
fnp-5883	661	125	j.	j.	PROPN
fnp-5883	661	126	,	,	PUNCT
fnp-5883	661	127	elliott	elliott	PROPN
fnp-5883	661	128	,	,	PUNCT
fnp-5883	661	129	j.a.w	j.a.w	PROPN
fnp-5883	661	130	.	.	PROPN
fnp-5883	661	131	,	,	PUNCT
fnp-5883	662	1	woodruff	woodruff	PROPN
fnp-5883	662	2	,	,	PUNCT
fnp-5883	662	3	t.k	t.k	PROPN
fnp-5883	662	4	.	.	PROPN
fnp-5883	662	5	,	,	PUNCT
fnp-5883	662	6	morris	morris	PROPN
fnp-5883	662	7	,	,	PUNCT
fnp-5883	662	8	g.j	g.j	PROPN
fnp-5883	662	9	.	.	PROPN
fnp-5883	662	10	,	,	PUNCT
fnp-5883	662	11	demirci	demirci	PROPN
fnp-5883	662	12	,	,	PUNCT
fnp-5883	662	13	u.	u.	PROPN
fnp-5883	662	14	,	,	PUNCT
fnp-5883	662	15	brockbank	brockbank	INTJ
fnp-5883	662	16	,	,	PUNCT
fnp-5883	662	17	k.g.m	k.g.m	PROPN
fnp-5883	662	18	.	.	PROPN
fnp-5883	662	19	,	,	PUNCT
fnp-5883	662	20	woods	woods	PROPN
fnp-5883	662	21	,	,	PUNCT
fnp-5883	662	22	e.j	e.j	PROPN
fnp-5883	662	23	.	.	PROPN
fnp-5883	662	24	,	,	PUNCT
fnp-5883	662	25	ben	ben	PROPN
fnp-5883	662	26	,	,	PUNCT
fnp-5883	662	27	r.n	r.n	PROPN
fnp-5883	662	28	.	.	PROPN
fnp-5883	662	29	,	,	PUNCT
fnp-5883	662	30	baust	baust	PROPN
fnp-5883	662	31	,	,	PUNCT
fnp-5883	662	32	j.g	j.g	PROPN
fnp-5883	662	33	.	.	PROPN
fnp-5883	662	34	,	,	PUNCT
fnp-5883	662	35	gao	gao	PROPN
fnp-5883	662	36	,	,	PUNCT
fnp-5883	662	37	d.	d.	PROPN
fnp-5883	662	38	,	,	PUNCT
fnp-5883	662	39	fuller	full	ADJ
fnp-5883	662	40	,	,	PUNCT
fnp-5883	662	41	b.	b.	PROPN
fnp-5883	662	42	,	,	PUNCT
fnp-5883	662	43	rabin	rabin	PROPN
fnp-5883	662	44	,	,	PUNCT
fnp-5883	662	45	y.	y.	PROPN
fnp-5883	662	46	,	,	PUNCT
fnp-5883	662	47	kravitz	kravitz	PROPN
fnp-5883	662	48	,	,	PUNCT
fnp-5883	662	49	d.c	d.c	PROPN
fnp-5883	662	50	.	.	PROPN
fnp-5883	662	51	,	,	PUNCT
fnp-5883	662	52	taylor	taylor	PROPN
fnp-5883	662	53	,	,	PUNCT
fnp-5883	662	54	m.j	m.j	PROPN
fnp-5883	662	55	.	.	PROPN
fnp-5883	662	56	,	,	PUNCT
fnp-5883	662	57	toner	toner	NOUN
fnp-5883	662	58	,	,	PUNCT
fnp-5883	662	59	m.	m.	NOUN
fnp-5883	662	60	,	,	PUNCT
fnp-5883	662	61	2017	2017	NUM
fnp-5883	662	62	.	.	PUNCT
fnp-5883	663	1	the	the	DET
fnp-5883	663	2	promise	promise	NOUN
fnp-5883	663	3	of	of	ADP
fnp-5883	663	4	organ	organ	NOUN
fnp-5883	663	5	and	and	CCONJ
fnp-5883	663	6	tissue	tissue	NOUN
fnp-5883	663	7	preservation	preservation	NOUN
fnp-5883	663	8	to	to	PART
fnp-5883	663	9	transform	transform	VERB
fnp-5883	663	10	medicine	medicine	NOUN
fnp-5883	663	11	.	.	PUNCT
fnp-5883	664	1	nat	nat	PROPN
fnp-5883	664	2	.	.	PUNCT
fnp-5883	665	1	biotechnol	biotechnol	PROPN
fnp-5883	665	2	.	.	PUNCT
fnp-5883	666	1	35	35	NUM
fnp-5883	666	2	,	,	PUNCT
fnp-5883	666	3	530–542	530–542	NUM
fnp-5883	666	4	.	.	PUNCT
fnp-5883	667	1	https://doi.org/10.1038/nbt.3889	https://doi.org/10.1038/nbt.3889	PROPN
fnp-5883	667	2	guo	guo	PROPN
fnp-5883	667	3	,	,	PUNCT
fnp-5883	667	4	j.	j.	PROPN
fnp-5883	667	5	,	,	PUNCT
fnp-5883	667	6	wang	wang	PROPN
fnp-5883	667	7	,	,	PUNCT
fnp-5883	667	8	g.	g.	PROPN
fnp-5883	667	9	,	,	PUNCT
fnp-5883	667	10	tang	tang	PROPN
fnp-5883	667	11	,	,	PUNCT
fnp-5883	667	12	w.	w.	PROPN
fnp-5883	667	13	,	,	PUNCT
fnp-5883	667	14	song	song	NOUN
fnp-5883	667	15	,	,	PUNCT
fnp-5883	667	16	d.	d.	PROPN
fnp-5883	667	17	,	,	PUNCT
fnp-5883	667	18	wang	wang	PROPN
fnp-5883	667	19	,	,	PUNCT
fnp-5883	667	20	x.	x.	PROPN
fnp-5883	667	21	,	,	PUNCT
fnp-5883	667	22	hong	hong	PROPN
fnp-5883	667	23	,	,	PUNCT
fnp-5883	667	24	j.	j.	PROPN
fnp-5883	667	25	,	,	PUNCT
fnp-5883	667	26	zhang	zhang	PROPN
fnp-5883	667	27	,	,	PUNCT
fnp-5883	667	28	z.	z.	PROPN
fnp-5883	667	29	,	,	PUNCT
fnp-5883	667	30	2020	2020	NUM
fnp-5883	667	31	.	.	PUNCT
fnp-5883	668	1	an	an	DET
fnp-5883	668	2	optimized	optimize	VERB
fnp-5883	668	3	approach	approach	NOUN
fnp-5883	668	4	using	use	VERB
fnp-5883	668	5	cryofixation	cryofixation	NOUN
fnp-5883	668	6	for	for	ADP
fnp-5883	668	7	high	high	ADJ
fnp-5883	668	8	-	-	PUNCT
fnp-5883	668	9	resolution	resolution	NOUN
fnp-5883	668	10	3d	3d	NUM
fnp-5883	668	11	analysis	analysis	NOUN
fnp-5883	668	12	by	by	ADP
fnp-5883	668	13	fib	fib	ADJ
fnp-5883	668	14	-	-	PUNCT
fnp-5883	668	15	sem	sem	NOUN
fnp-5883	668	16	.	.	PUNCT
fnp-5883	669	1	j.	j.	PROPN
fnp-5883	669	2	struct	struct	PROPN
fnp-5883	669	3	.	.	PUNCT
fnp-5883	670	1	biol	biol	PROPN
fnp-5883	670	2	.	.	PUNCT
fnp-5883	671	1	212	212	NUM
fnp-5883	671	2	,	,	PUNCT
fnp-5883	671	3	107600	107600	NUM
fnp-5883	671	4	.	.	PUNCT
fnp-5883	672	1	https://doi.org/10.1016/j.jsb.2020.107600	https://doi.org/10.1016/j.jsb.2020.107600	PROPN
fnp-5883	672	2	hagenauer	hagenauer	NOUN
fnp-5883	672	3	,	,	PUNCT
fnp-5883	672	4	m.h	m.h	PROPN
fnp-5883	672	5	.	.	PROPN
fnp-5883	672	6	,	,	PUNCT
fnp-5883	672	7	schulmann	schulmann	PROPN
fnp-5883	672	8	,	,	PUNCT
fnp-5883	672	9	a.	a.	PROPN
fnp-5883	672	10	,	,	PUNCT
fnp-5883	672	11	li	li	PROPN
fnp-5883	672	12	,	,	PUNCT
fnp-5883	672	13	j.z	j.z	PROPN
fnp-5883	672	14	.	.	PROPN
fnp-5883	672	15	,	,	PUNCT
fnp-5883	672	16	vawter	vawter	NOUN
fnp-5883	672	17	,	,	PUNCT
fnp-5883	672	18	m.p	m.p	PROPN
fnp-5883	672	19	.	.	PROPN
fnp-5883	672	20	,	,	PUNCT
fnp-5883	672	21	walsh	walsh	PROPN
fnp-5883	672	22	,	,	PUNCT
fnp-5883	672	23	d.m	d.m	PROPN
fnp-5883	672	24	.	.	PROPN
fnp-5883	672	25	,	,	PUNCT
fnp-5883	672	26	thompson	thompson	PROPN
fnp-5883	672	27	,	,	PUNCT
fnp-5883	672	28	r.c	r.c	PROPN
fnp-5883	672	29	.	.	PROPN
fnp-5883	672	30	,	,	PUNCT
fnp-5883	672	31	turner	turner	PROPN
fnp-5883	672	32	,	,	PUNCT
fnp-5883	672	33	c.a	c.a	PROPN
fnp-5883	672	34	.	.	PROPN
fnp-5883	672	35	,	,	PUNCT
fnp-5883	672	36	bunney	bunney	NOUN
fnp-5883	672	37	,	,	PUNCT
fnp-5883	672	38	w.e	w.e	PROPN
fnp-5883	672	39	.	.	PROPN
fnp-5883	672	40	,	,	PUNCT
fnp-5883	672	41	myers	myers	PROPN
fnp-5883	672	42	,	,	PUNCT
fnp-5883	672	43	r.m	r.m	PROPN
fnp-5883	672	44	.	.	PROPN
fnp-5883	672	45	,	,	PUNCT
fnp-5883	672	46	barchas	barchas	PROPN
fnp-5883	672	47	,	,	PUNCT
fnp-5883	672	48	j.d	j.d	PROPN
fnp-5883	672	49	.	.	PROPN
fnp-5883	672	50	,	,	PUNCT
fnp-5883	672	51	schatzberg	schatzberg	PROPN
fnp-5883	672	52	,	,	PUNCT
fnp-5883	672	53	a.f	a.f	PROPN
fnp-5883	672	54	.	.	PROPN
fnp-5883	672	55	,	,	PUNCT
fnp-5883	672	56	watson	watson	PROPN
fnp-5883	672	57	,	,	PUNCT
fnp-5883	672	58	s.j	s.j	PROPN
fnp-5883	672	59	.	.	PROPN
fnp-5883	672	60	,	,	PUNCT
fnp-5883	672	61	akil	akil	PROPN
fnp-5883	672	62	,	,	PUNCT
fnp-5883	672	63	h.	h.	PROPN
fnp-5883	672	64	,	,	PUNCT
fnp-5883	672	65	2018	2018	NUM
fnp-5883	672	66	.	.	PUNCT
fnp-5883	673	1	inference	inference	NOUN
fnp-5883	673	2	of	of	ADP
fnp-5883	673	3	cell	cell	NOUN
fnp-5883	673	4	type	type	NOUN
fnp-5883	673	5	content	content	NOUN
fnp-5883	673	6	from	from	ADP
fnp-5883	673	7	human	human	ADJ
fnp-5883	673	8	brain	brain	NOUN
fnp-5883	673	9	transcriptomic	transcriptomic	ADJ
fnp-5883	673	10	datasets	dataset	NOUN
fnp-5883	673	11	illuminates	illuminate	VERB
fnp-5883	673	12	the	the	DET
fnp-5883	673	13	effects	effect	NOUN
fnp-5883	673	14	of	of	ADP
fnp-5883	673	15	age	age	NOUN
fnp-5883	673	16	,	,	PUNCT
fnp-5883	673	17	manner	manner	NOUN
fnp-5883	673	18	of	of	ADP
fnp-5883	673	19	death	death	NOUN
fnp-5883	673	20	,	,	PUNCT
fnp-5883	673	21	dissection	dissection	NOUN
fnp-5883	673	22	,	,	PUNCT
fnp-5883	673	23	and	and	CCONJ
fnp-5883	673	24	psychiatric	psychiatric	ADJ
fnp-5883	673	25	diagnosis	diagnosis	NOUN
fnp-5883	673	26	.	.	PUNCT
fnp-5883	674	1	plos	plos	PROPN
fnp-5883	674	2	one	one	NUM
fnp-5883	674	3	13	13	NUM
fnp-5883	674	4	,	,	PUNCT
fnp-5883	674	5	e0200003	e0200003	PROPN
fnp-5883	674	6	.	.	PROPN
fnp-5883	675	1	https://doi.org/10.1371/journal.pone.0200003	https://doi.org/10.1371/journal.pone.0200003	PROPN
fnp-5883	675	2	han	han	PROPN
fnp-5883	675	3	,	,	PUNCT
fnp-5883	675	4	b.	b.	PROPN
fnp-5883	675	5	,	,	PUNCT
fnp-5883	675	6	bischof	bischof	NOUN
fnp-5883	675	7	,	,	PUNCT
fnp-5883	675	8	j.c	j.c	PROPN
fnp-5883	675	9	.	.	PROPN
fnp-5883	675	10	,	,	PUNCT
fnp-5883	675	11	2004	2004	NUM
fnp-5883	675	12	.	.	PUNCT
fnp-5883	676	1	direct	direct	ADJ
fnp-5883	676	2	cell	cell	NOUN
fnp-5883	676	3	injury	injury	NOUN
fnp-5883	676	4	associated	associate	VERB
fnp-5883	676	5	with	with	ADP
fnp-5883	676	6	eutectic	eutectic	ADJ
fnp-5883	676	7	crystallization	crystallization	NOUN
fnp-5883	676	8	during	during	ADP
fnp-5883	676	9	freezing	freezing	NOUN
fnp-5883	676	10	.	.	PUNCT
fnp-5883	677	1	cryobiology	cryobiology	NOUN
fnp-5883	677	2	48	48	NUM
fnp-5883	677	3	,	,	PUNCT
fnp-5883	677	4	8–21	8–21	NOUN
fnp-5883	677	5	.	.	PUNCT
fnp-5883	678	1	https://doi.org/10.1016/j.cryobiol.2003.11.002	https://doi.org/10.1016/j.cryobiol.2003.11.002	NOUN
fnp-5883	678	2	harreveld	harreveld	PROPN
fnp-5883	678	3	,	,	PUNCT
fnp-5883	678	4	a.v	a.v	PROPN
fnp-5883	678	5	.	.	PROPN
fnp-5883	678	6	,	,	PUNCT
fnp-5883	678	7	fifkova	fifkova	PROPN
fnp-5883	678	8	,	,	PUNCT
fnp-5883	678	9	e.	e.	PROPN
fnp-5883	678	10	,	,	PUNCT
fnp-5883	678	11	1975	1975	NUM
fnp-5883	678	12	.	.	PUNCT
fnp-5883	679	1	rapid	rapid	ADJ
fnp-5883	679	2	freezing	freezing	NOUN
fnp-5883	679	3	of	of	ADP
fnp-5883	679	4	deep	deep	ADJ
fnp-5883	679	5	cerebral	cerebral	ADJ
fnp-5883	679	6	structures	structure	NOUN
fnp-5883	679	7	for	for	ADP
fnp-5883	679	8	electron	electron	NOUN
fnp-5883	679	9	microscopy	microscopy	NOUN
fnp-5883	679	10	.	.	PUNCT
fnp-5883	680	1	anat	anat	PROPN
fnp-5883	680	2	.	.	PUNCT
fnp-5883	681	1	rec	rec	PROPN
fnp-5883	681	2	.	.	PROPN
fnp-5883	681	3	182	182	NUM
fnp-5883	681	4	,	,	PUNCT
fnp-5883	681	5	377–385	377–385	NUM
fnp-5883	681	6	.	.	PUNCT
fnp-5883	682	1	https://doi.org/10.1002/ar.1091820311	https://doi.org/10.1002/ar.1091820311	ADP
fnp-5883	682	2	heuser	heuser	PROPN
fnp-5883	682	3	,	,	PUNCT
fnp-5883	682	4	j.e	j.e	PROPN
fnp-5883	682	5	.	.	PROPN
fnp-5883	682	6	,	,	PUNCT
fnp-5883	682	7	reese	reese	PROPN
fnp-5883	682	8	,	,	PUNCT
fnp-5883	682	9	t.s	t.s	PROPN
fnp-5883	682	10	.	.	PROPN
fnp-5883	682	11	,	,	PUNCT
fnp-5883	682	12	landis	landis	PROPN
fnp-5883	682	13	,	,	PUNCT
fnp-5883	682	14	d.m	d.m	PROPN
fnp-5883	682	15	.	.	PROPN
fnp-5883	682	16	,	,	PUNCT
fnp-5883	682	17	1976	1976	NUM
fnp-5883	682	18	.	.	PUNCT
fnp-5883	683	1	preservation	preservation	NOUN
fnp-5883	683	2	of	of	ADP
fnp-5883	683	3	synaptic	synaptic	ADJ
fnp-5883	683	4	structure	structure	NOUN
fnp-5883	683	5	by	by	ADP
fnp-5883	683	6	rapid	rapid	ADJ
fnp-5883	683	7	freezing	freezing	NOUN
fnp-5883	683	8	.	.	PUNCT
fnp-5883	684	1	cold	cold	ADJ
fnp-5883	684	2	spring	spring	NOUN
fnp-5883	684	3	harb	harb	PROPN
fnp-5883	684	4	.	.	PUNCT
fnp-5883	685	1	symp	symp	PROPN
fnp-5883	685	2	.	.	PUNCT
fnp-5883	686	1	quant	quant	NOUN
fnp-5883	686	2	.	.	PUNCT
fnp-5883	687	1	biol	biol	PROPN
fnp-5883	687	2	.	.	PUNCT
fnp-5883	688	1	40	40	NUM
fnp-5883	688	2	,	,	PUNCT
fnp-5883	688	3	17–24	17–24	NUM
fnp-5883	688	4	.	.	PUNCT
fnp-5883	689	1	https://doi.org/10.1101/sqb.1976.040.01.004	https://doi.org/10.1101/sqb.1976.040.01.004	PROPN
fnp-5883	689	2	hirokawa	hirokawa	PROPN
fnp-5883	689	3	,	,	PUNCT
fnp-5883	689	4	n.	n.	NOUN
fnp-5883	689	5	,	,	PUNCT
fnp-5883	689	6	kirino	kirino	PROPN
fnp-5883	689	7	,	,	PUNCT
fnp-5883	689	8	t.	t.	PROPN
fnp-5883	689	9	,	,	PUNCT
fnp-5883	689	10	1980	1980	NUM
fnp-5883	689	11	.	.	PUNCT
fnp-5883	690	1	an	an	DET
fnp-5883	690	2	ultrastructural	ultrastructural	ADJ
fnp-5883	690	3	study	study	NOUN
fnp-5883	690	4	of	of	ADP
fnp-5883	690	5	nerve	nerve	NOUN
fnp-5883	690	6	and	and	CCONJ
fnp-5883	690	7	glial	glial	ADJ
fnp-5883	690	8	cells	cell	NOUN
fnp-5883	690	9	by	by	ADP
fnp-5883	690	10	freeze	freeze	NOUN
fnp-5883	690	11	-	-	PUNCT
fnp-5883	690	12	substitution	substitution	NOUN
fnp-5883	690	13	.	.	PUNCT
fnp-5883	691	1	j.	j.	PROPN
fnp-5883	691	2	neurocytol	neurocytol	PROPN
fnp-5883	691	3	.	.	PUNCT
fnp-5883	692	1	9	9	NUM
fnp-5883	692	2	,	,	PUNCT
fnp-5883	692	3	243–254	243–254	NUM
fnp-5883	692	4	.	.	PUNCT
fnp-5883	693	1	https://doi.org/10.1007/bf01205160	https://doi.org/10.1007/bf01205160	PROPN
fnp-5883	693	2	hisano	hisano	PROPN
fnp-5883	693	3	,	,	PUNCT
fnp-5883	693	4	s.	s.	PROPN
fnp-5883	693	5	,	,	PUNCT
fnp-5883	693	6	adachi	adachi	PROPN
fnp-5883	693	7	,	,	PUNCT
fnp-5883	693	8	t.	t.	PROPN
fnp-5883	693	9	,	,	PUNCT
fnp-5883	693	10	maegawa	maegawa	PROPN
fnp-5883	693	11	,	,	PUNCT
fnp-5883	693	12	m.	m.	NOUN
fnp-5883	693	13	,	,	PUNCT
fnp-5883	693	14	daikoku	daikoku	PROPN
fnp-5883	693	15	,	,	PUNCT
fnp-5883	693	16	s.	s.	PROPN
fnp-5883	693	17	,	,	PUNCT
fnp-5883	693	18	1986	1986	NUM
fnp-5883	693	19	.	.	PUNCT
fnp-5883	694	1	some	some	DET
fnp-5883	694	2	improvement	improvement	NOUN
fnp-5883	694	3	in	in	ADP
fnp-5883	694	4	tissue	tissue	NOUN
fnp-5883	694	5	preparation	preparation	NOUN
fnp-5883	694	6	and	and	CCONJ
fnp-5883	694	7	colloidal	colloidal	NOUN
fnp-5883	694	8	-	-	PUNCT
fnp-5883	694	9	gold	gold	NOUN
fnp-5883	694	10	immunolabeling	immunolabeling	NOUN
fnp-5883	694	11	for	for	ADP
fnp-5883	694	12	electron	electron	NOUN
fnp-5883	694	13	microscopy	microscopy	NOUN
fnp-5883	694	14	.	.	PUNCT
fnp-5883	695	1	am	be	AUX
fnp-5883	695	2	.	.	PUNCT
fnp-5883	696	1	j.	j.	PROPN
fnp-5883	696	2	anat	anat	PROPN
fnp-5883	696	3	.	.	PROPN
fnp-5883	697	1	175	175	NUM
fnp-5883	697	2	,	,	PUNCT
fnp-5883	697	3	245–266	245–266	NUM
fnp-5883	697	4	.	.	PUNCT
fnp-5883	698	1	https://doi.org/10.1002/aja.1001750210	https://doi.org/10.1002/aja.1001750210	PROPN
fnp-5883	698	2	hubel	hubel	PROPN
fnp-5883	698	3	,	,	PUNCT
fnp-5883	698	4	a.	a.	PROPN
fnp-5883	698	5	,	,	PUNCT
fnp-5883	698	6	spindler	spindler	PROPN
fnp-5883	698	7	,	,	PUNCT
fnp-5883	698	8	r.	r.	PROPN
fnp-5883	698	9	,	,	PUNCT
fnp-5883	698	10	skubitz	skubitz	PROPN
fnp-5883	698	11	,	,	PUNCT
fnp-5883	698	12	a.p.n	a.p.n	NOUN
fnp-5883	698	13	.	.	PROPN
fnp-5883	698	14	,	,	PUNCT
fnp-5883	698	15	2014	2014	NUM
fnp-5883	698	16	.	.	PUNCT
fnp-5883	699	1	storage	storage	NOUN
fnp-5883	699	2	of	of	ADP
fnp-5883	699	3	human	human	ADJ
fnp-5883	699	4	biospecimens	biospecimen	NOUN
fnp-5883	699	5	:	:	PUNCT
fnp-5883	699	6	selection	selection	NOUN
fnp-5883	699	7	of	of	ADP
fnp-5883	699	8	the	the	DET
fnp-5883	699	9	optimal	optimal	ADJ
fnp-5883	699	10	storage	storage	NOUN
fnp-5883	699	11	temperature	temperature	NOUN
fnp-5883	699	12	.	.	PUNCT
fnp-5883	700	1	biopreservation	biopreservation	NOUN
fnp-5883	700	2	biobanking	biobanke	VERB
fnp-5883	700	3	12	12	NUM
fnp-5883	700	4	,	,	PUNCT
fnp-5883	700	5	165–175	165–175	NUM
fnp-5883	700	6	.	.	PUNCT
fnp-5883	701	1	https://doi.org/10.1089/bio.2013.0084	https://doi.org/10.1089/bio.2013.0084	ADJ
fnp-5883	701	2	hunt	hunt	PROPN
fnp-5883	701	3	,	,	PUNCT
fnp-5883	701	4	c.j	c.j	PROPN
fnp-5883	701	5	.	.	PROPN
fnp-5883	701	6	,	,	PUNCT
fnp-5883	701	7	song	song	NOUN
fnp-5883	701	8	,	,	PUNCT
fnp-5883	701	9	y.c	y.c	PROPN
fnp-5883	701	10	.	.	PROPN
fnp-5883	701	11	,	,	PUNCT
fnp-5883	701	12	bateson	bateson	PROPN
fnp-5883	701	13	,	,	PUNCT
fnp-5883	701	14	e.a	e.a	PROPN
fnp-5883	701	15	.	.	PROPN
fnp-5883	701	16	,	,	PUNCT
fnp-5883	701	17	pegg	pegg	PROPN
fnp-5883	701	18	,	,	PUNCT
fnp-5883	701	19	d.e	d.e	PROPN
fnp-5883	701	20	.	.	PROPN
fnp-5883	701	21	,	,	PUNCT
fnp-5883	701	22	1994	1994	NUM
fnp-5883	701	23	.	.	PUNCT
fnp-5883	702	1	fractures	fracture	NOUN
fnp-5883	702	2	in	in	ADP
fnp-5883	702	3	cryopreserved	cryopreserved	ADJ
fnp-5883	702	4	arteries	artery	NOUN
fnp-5883	702	5	.	.	PUNCT
fnp-5883	703	1	cryobiology	cryobiology	NOUN
fnp-5883	703	2	31	31	NUM
fnp-5883	703	3	,	,	PUNCT
fnp-5883	703	4	506–515	506–515	NUM
fnp-5883	703	5	.	.	PUNCT
fnp-5883	704	1	https://doi.org/10.1006/cryo.1994.1061	https://doi.org/10.1006/cryo.1994.1061	PROPN
fnp-5883	704	2	hynd	hynd	PROPN
fnp-5883	704	3	,	,	PUNCT
fnp-5883	704	4	m.r	m.r	PROPN
fnp-5883	704	5	.	.	PROPN
fnp-5883	704	6	,	,	PUNCT
fnp-5883	704	7	lewohl	lewohl	NOUN
fnp-5883	704	8	,	,	PUNCT
fnp-5883	704	9	j.m	j.m	PROPN
fnp-5883	704	10	.	.	PROPN
fnp-5883	704	11	,	,	PUNCT
fnp-5883	704	12	scott	scott	PROPN
fnp-5883	704	13	,	,	PUNCT
fnp-5883	704	14	h.l	h.l	PROPN
fnp-5883	704	15	.	.	PROPN
fnp-5883	704	16	,	,	PUNCT
fnp-5883	704	17	dodd	dodd	PROPN
fnp-5883	704	18	,	,	PUNCT
fnp-5883	704	19	p.r	p.r	PROPN
fnp-5883	704	20	.	.	PROPN
fnp-5883	704	21	,	,	PUNCT
fnp-5883	704	22	2003	2003	NUM
fnp-5883	704	23	.	.	PUNCT
fnp-5883	705	1	biochemical	biochemical	ADJ
fnp-5883	705	2	and	and	CCONJ
fnp-5883	705	3	molecular	molecular	ADJ
fnp-5883	705	4	studies	study	NOUN
fnp-5883	705	5	using	use	VERB
fnp-5883	705	6	human	human	ADJ
fnp-5883	705	7	autopsy	autopsy	ADJ
fnp-5883	705	8	brain	brain	NOUN
fnp-5883	705	9	tissue	tissue	NOUN
fnp-5883	705	10	.	.	PUNCT
fnp-5883	706	1	j.	j.	PROPN
fnp-5883	706	2	neurochem	neurochem	PROPN
fnp-5883	706	3	.	.	PUNCT
fnp-5883	707	1	85	85	NUM
fnp-5883	707	2	,	,	PUNCT
fnp-5883	707	3	543–562	543–562	NUM
fnp-5883	707	4	.	.	PUNCT
fnp-5883	707	5	https://doi.org/10.1046/j.1471-4159.2003.01747.x	https://doi.org/10.1046/j.1471-4159.2003.01747.x	NOUN
fnp-5883	707	6	itoyama	itoyama	NOUN
fnp-5883	707	7	,	,	PUNCT
fnp-5883	707	8	y.	y.	PROPN
fnp-5883	707	9	,	,	PUNCT
fnp-5883	707	10	sternberger	sternberger	PROPN
fnp-5883	707	11	,	,	PUNCT
fnp-5883	707	12	n.h	n.h	PROPN
fnp-5883	707	13	.	.	PROPN
fnp-5883	707	14	,	,	PUNCT
fnp-5883	707	15	kies	kies	PROPN
fnp-5883	707	16	,	,	PUNCT
fnp-5883	707	17	m.w	m.w	PROPN
fnp-5883	707	18	.	.	PROPN
fnp-5883	707	19	,	,	PUNCT
fnp-5883	707	20	cohen	cohen	PROPN
fnp-5883	707	21	,	,	PUNCT
fnp-5883	707	22	s.r	s.r	PROPN
fnp-5883	707	23	.	.	PROPN
fnp-5883	707	24	,	,	PUNCT
fnp-5883	707	25	richardson	richardson	PROPN
fnp-5883	707	26	,	,	PUNCT
fnp-5883	707	27	e.p	e.p	PROPN
fnp-5883	707	28	.	.	PROPN
fnp-5883	707	29	,	,	PUNCT
fnp-5883	707	30	webster	webster	PROPN
fnp-5883	707	31	,	,	PUNCT
fnp-5883	707	32	h.	h.	PROPN
fnp-5883	707	33	,	,	PUNCT
fnp-5883	707	34	1980	1980	NUM
fnp-5883	707	35	.	.	PUNCT
fnp-5883	708	1	immunocytochemical	immunocytochemical	ADJ
fnp-5883	708	2	method	method	NOUN
fnp-5883	708	3	to	to	PART
fnp-5883	708	4	identify	identify	VERB
fnp-5883	708	5	myelin	myelin	ADJ
fnp-5883	708	6	basic	basic	ADJ
fnp-5883	708	7	protein	protein	NOUN
fnp-5883	708	8	in	in	ADP
fnp-5883	708	9	oligodendroglia	oligodendroglia	NOUN
fnp-5883	708	10	and	and	CCONJ
fnp-5883	708	11	myelin	myelin	NOUN
fnp-5883	708	12	sheaths	sheath	NOUN
fnp-5883	708	13	of	of	ADP
fnp-5883	708	14	the	the	DET
fnp-5883	708	15	human	human	ADJ
fnp-5883	708	16	nervous	nervous	ADJ
fnp-5883	708	17	system	system	NOUN
fnp-5883	708	18	.	.	PUNCT
fnp-5883	709	1	ann	ann	PROPN
fnp-5883	709	2	.	.	PUNCT
fnp-5883	710	1	neurol	neurol	PROPN
fnp-5883	710	2	.	.	PUNCT
fnp-5883	711	1	7	7	NUM
fnp-5883	711	2	,	,	PUNCT
fnp-5883	711	3	157–166	157–166	NUM
fnp-5883	711	4	.	.	PUNCT
fnp-5883	712	1	https://doi.org/10.1002/ana.410070211	https://doi.org/10.1002/ana.410070211	PROPN
fnp-5883	712	2	jain	jain	PROPN
fnp-5883	712	3	,	,	PUNCT
fnp-5883	712	4	k.	k.	PROPN
fnp-5883	712	5	,	,	PUNCT
fnp-5883	712	6	salamat	salamat	NOUN
fnp-5883	712	7	-	-	PUNCT
fnp-5883	712	8	miller	miller	PROPN
fnp-5883	712	9	,	,	PUNCT
fnp-5883	712	10	n.	n.	PROPN
fnp-5883	712	11	,	,	PUNCT
fnp-5883	712	12	taylor	taylor	PROPN
fnp-5883	712	13	,	,	PUNCT
fnp-5883	712	14	k.	k.	PROPN
fnp-5883	712	15	,	,	PUNCT
fnp-5883	712	16	2021	2021	NUM
fnp-5883	712	17	.	.	PUNCT
fnp-5883	713	1	freeze	freeze	NOUN
fnp-5883	713	2	-	-	PUNCT
fnp-5883	713	3	thaw	thaw	NOUN
fnp-5883	713	4	characterization	characterization	NOUN
fnp-5883	713	5	process	process	NOUN
fnp-5883	713	6	to	to	PART
fnp-5883	713	7	minimize	minimize	VERB
fnp-5883	713	8	aggregation	aggregation	NOUN
fnp-5883	713	9	and	and	CCONJ
fnp-5883	713	10	enable	enable	ADJ
fnp-5883	713	11	drug	drug	NOUN
fnp-5883	713	12	product	product	NOUN
fnp-5883	713	13	manufacturing	manufacturing	NOUN
fnp-5883	713	14	of	of	ADP
fnp-5883	713	15	protein	protein	NOUN
fnp-5883	713	16	based	base	VERB
fnp-5883	713	17	therapeutics	therapeutic	NOUN
fnp-5883	713	18	.	.	PUNCT
fnp-5883	714	1	sci	sci	PROPN
fnp-5883	714	2	.	.	PROPN
fnp-5883	714	3	rep	rep	PROPN
fnp-5883	714	4	.	.	PROPN
fnp-5883	714	5	11	11	NUM
fnp-5883	714	6	,	,	PUNCT
fnp-5883	714	7	11332	11332	NUM
fnp-5883	714	8	.	.	PUNCT
fnp-5883	715	1	https://doi.org/10.1038/s41598-021-90772-9	https://doi.org/10.1038/s41598-021-90772-9	NOUN
fnp-5883	715	2	jones	jones	PROPN
fnp-5883	715	3	,	,	PUNCT
fnp-5883	715	4	e.g.	e.g.	ADV
fnp-5883	715	5	,	,	PUNCT
fnp-5883	715	6	hendry	hendry	PROPN
fnp-5883	715	7	,	,	PUNCT
fnp-5883	715	8	s.h.c	s.h.c	VERB
fnp-5883	715	9	.	.	PROPN
fnp-5883	715	10	,	,	PUNCT
fnp-5883	715	11	liu	liu	PROPN
fnp-5883	715	12	,	,	PUNCT
fnp-5883	715	13	x.-b	x.-b	PROPN
fnp-5883	715	14	.	.	PROPN
fnp-5883	715	15	,	,	PUNCT
fnp-5883	715	16	hodgins	hodgins	PROPN
fnp-5883	715	17	,	,	PUNCT
fnp-5883	715	18	s.	s.	PROPN
fnp-5883	715	19	,	,	PUNCT
fnp-5883	715	20	potkin	potkin	PROPN
fnp-5883	715	21	,	,	PUNCT
fnp-5883	715	22	s.g	s.g	PROPN
fnp-5883	715	23	.	.	PROPN
fnp-5883	715	24	,	,	PUNCT
fnp-5883	715	25	tourtellotte	tourtellotte	PROPN
fnp-5883	715	26	,	,	PUNCT
fnp-5883	715	27	w.w	w.w	PROPN
fnp-5883	715	28	.	.	PROPN
fnp-5883	715	29	,	,	PUNCT
fnp-5883	715	30	1992	1992	NUM
fnp-5883	715	31	.	.	PUNCT
fnp-5883	716	1	a	a	DET
fnp-5883	716	2	method	method	NOUN
fnp-5883	716	3	for	for	ADP
fnp-5883	716	4	fixation	fixation	NOUN
fnp-5883	716	5	of	of	ADP
fnp-5883	716	6	previously	previously	ADV
fnp-5883	716	7	fresh	fresh	ADJ
fnp-5883	716	8	-	-	PUNCT
fnp-5883	716	9	frozen	frozen	ADJ
fnp-5883	716	10	human	human	ADJ
fnp-5883	716	11	adult	adult	NOUN
fnp-5883	716	12	and	and	CCONJ
fnp-5883	716	13	fetal	fetal	ADJ
fnp-5883	716	14	brains	brain	NOUN
fnp-5883	716	15	that	that	PRON
fnp-5883	716	16	preserves	preserve	VERB
fnp-5883	716	17	histological	histological	ADJ
fnp-5883	716	18	quality	quality	NOUN
fnp-5883	716	19	and	and	CCONJ
fnp-5883	716	20	immunoreactivity	immunoreactivity	NOUN
fnp-5883	716	21	.	.	PUNCT
fnp-5883	717	1	j.	j.	PROPN
fnp-5883	717	2	neurosci	neurosci	PROPN
fnp-5883	717	3	.	.	PUNCT
fnp-5883	718	1	methods	method	NOUN
fnp-5883	718	2	44	44	NUM
fnp-5883	718	3	,	,	PUNCT
fnp-5883	718	4	133–144	133–144	NUM
fnp-5883	718	5	.	.	PUNCT
fnp-5883	719	1	https://doi.org/10.1016/0165-0270(92)90006-y	https://doi.org/10.1016/0165-0270(92)90006-y	PROPN
fnp-5883	719	2	kagan	kagan	PROPN
fnp-5883	719	3	,	,	PUNCT
fnp-5883	719	4	r.	r.	PROPN
fnp-5883	719	5	,	,	PUNCT
fnp-5883	719	6	viner	viner	PROPN
fnp-5883	719	7	,	,	PUNCT
fnp-5883	719	8	t.c	t.c	PROPN
fnp-5883	719	9	.	.	PROPN
fnp-5883	719	10	,	,	PUNCT
fnp-5883	719	11	2022	2022	NUM
fnp-5883	719	12	.	.	PUNCT
fnp-5883	720	1	identification	identification	NOUN
fnp-5883	720	2	of	of	ADP
fnp-5883	720	3	freeze	freeze	ADJ
fnp-5883	720	4	-	-	PUNCT
fnp-5883	720	5	thaw	thaw	NOUN
fnp-5883	720	6	artifact	artifact	NOUN
fnp-5883	720	7	in	in	ADP
fnp-5883	720	8	fresh	fresh	ADJ
fnp-5883	720	9	and	and	CCONJ
fnp-5883	720	10	decomposed	decompose	VERB
fnp-5883	720	11	black	black	ADJ
fnp-5883	720	12	rockfish	rockfish	NOUN
fnp-5883	720	13	(	(	PUNCT
fnp-5883	720	14	sebastes	sebaste	NOUN
fnp-5883	720	15	melanops	melanop	NOUN
fnp-5883	720	16	)	)	PUNCT
fnp-5883	720	17	and	and	CCONJ
fnp-5883	720	18	steelhead	steelhead	ADJ
fnp-5883	720	19	trout	trout	NOUN
fnp-5883	720	20	(	(	PUNCT
fnp-5883	720	21	oncorhynchus	oncorhynchus	NOUN
fnp-5883	720	22	mykiss	mykiss	NOUN
fnp-5883	720	23	)	)	PUNCT
fnp-5883	720	24	.	.	PUNCT
fnp-5883	721	1	vet	vet	NOUN
fnp-5883	721	2	.	.	PUNCT
fnp-5883	721	3	pathol	pathol	PROPN
fnp-5883	721	4	.	.	PUNCT
fnp-5883	722	1	59	59	NUM
fnp-5883	722	2	,	,	PUNCT
fnp-5883	722	3	1022–1030	1022–1030	NUM
fnp-5883	722	4	.	.	PUNCT
fnp-5883	723	1	https://doi.org/10.1177/03009858221120012	https://doi.org/10.1177/03009858221120012	PROPN
fnp-5883	723	2	karlsson	karlsson	PROPN
fnp-5883	723	3	,	,	PUNCT
fnp-5883	723	4	j.o	j.o	PROPN
fnp-5883	723	5	.	.	PROPN
fnp-5883	723	6	,	,	PUNCT
fnp-5883	723	7	toner	toner	NOUN
fnp-5883	723	8	,	,	PUNCT
fnp-5883	723	9	m.	m.	NOUN
fnp-5883	723	10	,	,	PUNCT
fnp-5883	723	11	1996	1996	NUM
fnp-5883	723	12	.	.	PUNCT
fnp-5883	724	1	long	long	ADJ
fnp-5883	724	2	-	-	PUNCT
fnp-5883	724	3	term	term	NOUN
fnp-5883	724	4	storage	storage	NOUN
fnp-5883	724	5	of	of	ADP
fnp-5883	724	6	tissues	tissue	NOUN
fnp-5883	724	7	by	by	ADP
fnp-5883	724	8	cryopreservation	cryopreservation	NOUN
fnp-5883	724	9	:	:	PUNCT
fnp-5883	724	10	critical	critical	ADJ
fnp-5883	724	11	issues	issue	NOUN
fnp-5883	724	12	.	.	PUNCT
fnp-5883	725	1	biomaterials	biomaterial	NOUN
fnp-5883	725	2	17	17	NUM
fnp-5883	725	3	,	,	PUNCT
fnp-5883	725	4	243–256	243–256	NUM
fnp-5883	725	5	.	.	PUNCT
fnp-5883	726	1	https://doi.org/10.1016/0142-9612(96)85562-1	https://doi.org/10.1016/0142-9612(96)85562-1	PROPN
fnp-5883	726	2	kirschner	kirschner	PROPN
fnp-5883	726	3	,	,	PUNCT
fnp-5883	726	4	d.a	d.a	PROPN
fnp-5883	726	5	.	.	PROPN
fnp-5883	726	6	,	,	PUNCT
fnp-5883	726	7	hollingshead	hollingshead	ADJ
fnp-5883	726	8	,	,	PUNCT
fnp-5883	726	9	c.j	c.j	PROPN
fnp-5883	726	10	.	.	PROPN
fnp-5883	726	11	,	,	PUNCT
fnp-5883	726	12	thaxton	thaxton	PROPN
fnp-5883	726	13	,	,	PUNCT
fnp-5883	726	14	c.	c.	PROPN
fnp-5883	726	15	,	,	PUNCT
fnp-5883	726	16	caspar	caspar	PROPN
fnp-5883	726	17	,	,	PUNCT
fnp-5883	726	18	d.l	d.l	PROPN
fnp-5883	726	19	.	.	PROPN
fnp-5883	726	20	,	,	PUNCT
fnp-5883	726	21	goodenough	goodenough	PROPN
fnp-5883	726	22	,	,	PUNCT
fnp-5883	726	23	d.a	d.a	PROPN
fnp-5883	726	24	.	.	PROPN
fnp-5883	726	25	,	,	PUNCT
fnp-5883	726	26	1979	1979	NUM
fnp-5883	726	27	.	.	PUNCT
fnp-5883	727	1	structural	structural	ADJ
fnp-5883	727	2	states	state	NOUN
fnp-5883	727	3	of	of	ADP
fnp-5883	727	4	myelin	myelin	NOUN
fnp-5883	727	5	observed	observe	VERB
fnp-5883	727	6	by	by	ADP
fnp-5883	727	7	x	x	NOUN
fnp-5883	727	8	-	-	NOUN
fnp-5883	727	9	ray	ray	NOUN
fnp-5883	727	10	diffraction	diffraction	NOUN
fnp-5883	727	11	and	and	CCONJ
fnp-5883	727	12	freeze	freeze	ADJ
fnp-5883	727	13	-	-	PUNCT
fnp-5883	727	14	fracture	fracture	NOUN
fnp-5883	727	15	electron	electron	NOUN
fnp-5883	727	16	microscopy	microscopy	NOUN
fnp-5883	727	17	.	.	PUNCT
fnp-5883	728	1	j.	j.	PROPN
fnp-5883	728	2	cell	cell	PROPN
fnp-5883	728	3	biol	biol	PROPN
fnp-5883	728	4	.	.	PUNCT
fnp-5883	729	1	82	82	NUM
fnp-5883	729	2	,	,	PUNCT
fnp-5883	729	3	140–149	140–149	NUM
fnp-5883	729	4	.	.	PUNCT
fnp-5883	730	1	https://doi.org/10.1083/jcb.82.1.140	https://doi.org/10.1083/jcb.82.1.140	PROPN
fnp-5883	730	2	klbik	klbik	PROPN
fnp-5883	730	3	,	,	PUNCT
fnp-5883	730	4	i.	i.	PROPN
fnp-5883	730	5	,	,	PUNCT
fnp-5883	730	6	čechová	čechová	PROPN
fnp-5883	730	7	,	,	PUNCT
fnp-5883	730	8	k.	k.	PROPN
fnp-5883	730	9	,	,	PUNCT
fnp-5883	730	10	milovská	milovská	PROPN
fnp-5883	730	11	,	,	PUNCT
fnp-5883	730	12	s.	s.	PROPN
fnp-5883	730	13	,	,	PUNCT
fnp-5883	730	14	rusnák	rusnák	PROPN
fnp-5883	730	15	,	,	PUNCT
fnp-5883	730	16	j.	j.	PROPN
fnp-5883	730	17	,	,	PUNCT
fnp-5883	730	18	vlasáč	vlasáč	NOUN
fnp-5883	730	19	,	,	PUNCT
fnp-5883	730	20	j.	j.	PROPN
fnp-5883	730	21	,	,	PUNCT
fnp-5883	730	22	melicherčík	melicherčík	NOUN
fnp-5883	730	23	,	,	PUNCT
fnp-5883	730	24	m.	m.	NOUN
fnp-5883	730	25	,	,	PUNCT
fnp-5883	730	26	mat’ko	mat’ko	NOUN
fnp-5883	730	27	,	,	PUNCT
fnp-5883	730	28	i.	i.	PROPN
fnp-5883	730	29	,	,	PUNCT
fnp-5883	730	30	lakota	lakota	PROPN
fnp-5883	730	31	,	,	PUNCT
fnp-5883	730	32	j.	j.	PROPN
fnp-5883	730	33	,	,	PUNCT
fnp-5883	730	34	šauša	šauša	PROPN
fnp-5883	730	35	,	,	PUNCT
fnp-5883	730	36	o.	o.	NOUN
fnp-5883	730	37	,	,	PUNCT
fnp-5883	730	38	2022	2022	NUM
fnp-5883	730	39	.	.	PUNCT
fnp-5883	731	1	cryoprotective	cryoprotective	ADJ
fnp-5883	731	2	mechanism	mechanism	NOUN
fnp-5883	731	3	of	of	ADP
fnp-5883	731	4	dmso	dmso	NOUN
fnp-5883	731	5	induced	induce	VERB
fnp-5883	731	6	by	by	ADP
fnp-5883	731	7	the	the	DET
fnp-5883	731	8	inhibitory	inhibitory	ADJ
fnp-5883	731	9	effect	effect	NOUN
fnp-5883	731	10	on	on	ADP
fnp-5883	731	11	eutectic	eutectic	ADJ
fnp-5883	731	12	nacl	nacl	NOUN
fnp-5883	731	13	crystallization	crystallization	NOUN
fnp-5883	731	14	.	.	PUNCT
fnp-5883	732	1	j.	j.	PROPN
fnp-5883	732	2	phys	phys	PROPN
fnp-5883	732	3	.	.	PUNCT
fnp-5883	733	1	chem	chem	PROPN
fnp-5883	733	2	.	.	PUNCT
fnp-5883	734	1	lett	lett	PROPN
fnp-5883	734	2	.	.	PROPN
fnp-5883	735	1	13	13	NUM
fnp-5883	735	2	,	,	PUNCT
fnp-5883	735	3	11153–11159	11153–11159	NUM
fnp-5883	735	4	.	.	PUNCT
fnp-5883	736	1	https://doi.org/10.1021/acs.jpclett.2c03003	https://doi.org/10.1021/acs.jpclett.2c03003	PRON
fnp-5883	736	2	korogod	korogod	PROPN
fnp-5883	736	3	,	,	PUNCT
fnp-5883	736	4	n.	n.	NOUN
fnp-5883	736	5	,	,	PUNCT
fnp-5883	736	6	petersen	petersen	PROPN
fnp-5883	736	7	,	,	PUNCT
fnp-5883	736	8	c.c	c.c	PROPN
fnp-5883	736	9	.	.	PROPN
fnp-5883	736	10	,	,	PUNCT
fnp-5883	736	11	knott	knott	PROPN
fnp-5883	736	12	,	,	PUNCT
fnp-5883	736	13	g.w	g.w	PROPN
fnp-5883	736	14	.	.	PROPN
fnp-5883	736	15	,	,	PUNCT
fnp-5883	736	16	2015	2015	NUM
fnp-5883	736	17	.	.	PUNCT
fnp-5883	737	1	ultrastructural	ultrastructural	ADJ
fnp-5883	737	2	analysis	analysis	NOUN
fnp-5883	737	3	of	of	ADP
fnp-5883	737	4	adult	adult	NOUN
fnp-5883	737	5	mouse	mouse	NOUN
fnp-5883	737	6	neocortex	neocortex	NOUN
fnp-5883	737	7	comparing	compare	VERB
fnp-5883	737	8	aldehyde	aldehyde	NOUN
fnp-5883	737	9	perfusion	perfusion	NOUN
fnp-5883	737	10	with	with	ADP
fnp-5883	737	11	cryo	cryo	NOUN
fnp-5883	737	12	fixation	fixation	NOUN
fnp-5883	737	13	.	.	PUNCT
fnp-5883	738	1	elife	elife	NOUN
fnp-5883	738	2	4	4	NUM
fnp-5883	738	3	,	,	PUNCT
fnp-5883	738	4	e05793	e05793	ADJ
fnp-5883	738	5	.	.	PUNCT
fnp-5883	739	1	https://doi.org/10.7554/elife.05793	https://doi.org/10.7554/elife.05793	ADJ
fnp-5883	739	2	krassner	krassner	NOUN
fnp-5883	739	3	,	,	PUNCT
fnp-5883	739	4	m.m	m.m	PROPN
fnp-5883	739	5	.	.	PROPN
fnp-5883	739	6	,	,	PUNCT
fnp-5883	739	7	kauffman	kauffman	PROPN
fnp-5883	739	8	,	,	PUNCT
fnp-5883	739	9	j.	j.	PROPN
fnp-5883	739	10	,	,	PUNCT
fnp-5883	739	11	sowa	sowa	PROPN
fnp-5883	739	12	,	,	PUNCT
fnp-5883	739	13	a.	a.	PROPN
fnp-5883	739	14	,	,	PUNCT
fnp-5883	739	15	cialowicz	cialowicz	PROPN
fnp-5883	739	16	,	,	PUNCT
fnp-5883	739	17	k.	k.	PROPN
fnp-5883	739	18	,	,	PUNCT
fnp-5883	739	19	walsh	walsh	PROPN
fnp-5883	739	20	,	,	PUNCT
fnp-5883	739	21	s.	s.	PROPN
fnp-5883	739	22	,	,	PUNCT
fnp-5883	739	23	farrell	farrell	PROPN
fnp-5883	739	24	,	,	PUNCT
fnp-5883	739	25	k.	k.	PROPN
fnp-5883	739	26	,	,	PUNCT
fnp-5883	739	27	crary	crary	PROPN
fnp-5883	739	28	,	,	PUNCT
fnp-5883	739	29	j.f	j.f	PROPN
fnp-5883	739	30	.	.	PROPN
fnp-5883	739	31	,	,	PUNCT
fnp-5883	739	32	mckenzie	mckenzie	PROPN
fnp-5883	739	33	,	,	PUNCT
fnp-5883	739	34	a.t	a.t	PROPN
fnp-5883	739	35	.	.	PROPN
fnp-5883	739	36	,	,	PUNCT
fnp-5883	739	37	2023	2023	NUM
fnp-5883	739	38	.	.	PUNCT
fnp-5883	740	1	postmortem	postmortem	NOUN
fnp-5883	740	2	changes	change	NOUN
fnp-5883	740	3	in	in	ADP
fnp-5883	740	4	brain	brain	NOUN
fnp-5883	740	5	cell	cell	NOUN
fnp-5883	740	6	structure	structure	NOUN
fnp-5883	740	7	:	:	PUNCT
fnp-5883	740	8	a	a	DET
fnp-5883	740	9	review	review	NOUN
fnp-5883	740	10	.	.	PUNCT
fnp-5883	741	1	free	free	ADJ
fnp-5883	741	2	neuropathol	neuropathol	NOUN
fnp-5883	741	3	.	.	PUNCT
fnp-5883	742	1	4	4	NUM
fnp-5883	742	2	,	,	PUNCT
fnp-5883	742	3	4–10	4–10	NOUN
fnp-5883	742	4	.	.	PUNCT
fnp-5883	743	1	https://doi.org/10.17879/freeneuropathology-2023-4790	https://doi.org/10.17879/freeneuropathology-2023-4790	NOUN
fnp-5883	743	2	kumarasami	kumarasami	NOUN
fnp-5883	743	3	,	,	PUNCT
fnp-5883	743	4	r.	r.	PROPN
fnp-5883	743	5	,	,	PUNCT
fnp-5883	743	6	verma	verma	PROPN
fnp-5883	743	7	,	,	PUNCT
fnp-5883	743	8	r.	r.	PROPN
fnp-5883	743	9	,	,	PUNCT
fnp-5883	743	10	pandurangan	pandurangan	NOUN
fnp-5883	743	11	,	,	PUNCT
fnp-5883	743	12	k.	k.	PROPN
fnp-5883	743	13	,	,	PUNCT
fnp-5883	743	14	ramesh	ramesh	PROPN
fnp-5883	743	15	,	,	PUNCT
fnp-5883	743	16	j.j	j.j	PROPN
fnp-5883	743	17	.	.	PROPN
fnp-5883	743	18	,	,	PUNCT
fnp-5883	743	19	pandidurai	pandidurai	PROPN
fnp-5883	743	20	,	,	PUNCT
fnp-5883	743	21	s.	s.	PROPN
fnp-5883	743	22	,	,	PUNCT
fnp-5883	743	23	savoia	savoia	PROPN
fnp-5883	743	24	,	,	PUNCT
fnp-5883	743	25	s.	s.	PROPN
fnp-5883	743	26	,	,	PUNCT
fnp-5883	743	27	jayakumar	jayakumar	PROPN
fnp-5883	743	28	,	,	PUNCT
fnp-5883	743	29	j.	j.	PROPN
fnp-5883	743	30	,	,	PUNCT
fnp-5883	743	31	bota	bota	NOUN
fnp-5883	743	32	,	,	PUNCT
fnp-5883	743	33	m.	m.	NOUN
fnp-5883	743	34	,	,	PUNCT
fnp-5883	743	35	mitra	mitra	PROPN
fnp-5883	743	36	,	,	PUNCT
fnp-5883	743	37	p.	p.	PROPN
fnp-5883	743	38	,	,	PUNCT
fnp-5883	743	39	joseph	joseph	PROPN
fnp-5883	743	40	,	,	PUNCT
fnp-5883	743	41	j.	j.	PROPN
fnp-5883	743	42	,	,	PUNCT
fnp-5883	743	43	sivaprakasam	sivaprakasam	PROPN
fnp-5883	743	44	,	,	PUNCT
fnp-5883	743	45	m.	m.	NOUN
fnp-5883	743	46	,	,	PUNCT
fnp-5883	743	47	2023	2023	NUM
fnp-5883	743	48	.	.	PUNCT
fnp-5883	744	1	a	a	DET
fnp-5883	744	2	technology	technology	NOUN
fnp-5883	744	3	platform	platform	NOUN
fnp-5883	744	4	for	for	ADP
fnp-5883	744	5	standardized	standardized	ADJ
fnp-5883	744	6	cryoprotection	cryoprotection	NOUN
fnp-5883	744	7	and	and	CCONJ
fnp-5883	744	8	freezing	freezing	NOUN
fnp-5883	744	9	of	of	ADP
fnp-5883	744	10	large	large	ADJ
fnp-5883	744	11	-	-	PUNCT
fnp-5883	744	12	volume	volume	NOUN
fnp-5883	744	13	brain	brain	NOUN
fnp-5883	744	14	tissues	tissue	NOUN
fnp-5883	744	15	for	for	ADP
fnp-5883	744	16	high	high	ADJ
fnp-5883	744	17	-	-	PUNCT
fnp-5883	744	18	resolution	resolution	NOUN
fnp-5883	744	19	histology	histology	NOUN
fnp-5883	744	20	.	.	PUNCT
fnp-5883	745	1	front	front	PROPN
fnp-5883	745	2	.	.	PUNCT
fnp-5883	746	1	neuroanat	neuroanat	PROPN
fnp-5883	746	2	.	.	PUNCT
fnp-5883	747	1	17	17	NUM
fnp-5883	747	2	,	,	PUNCT
fnp-5883	747	3	1292655	1292655	NUM
fnp-5883	747	4	.	.	PUNCT
fnp-5883	748	1	https://doi.org/10.3389/fnana.2023.1292655	https://doi.org/10.3389/fnana.2023.1292655	PROPN
fnp-5883	748	2	landis	landis	PROPN
fnp-5883	748	3	,	,	PUNCT
fnp-5883	748	4	d.m	d.m	PROPN
fnp-5883	748	5	.	.	PROPN
fnp-5883	748	6	,	,	PUNCT
fnp-5883	748	7	reese	reese	PROPN
fnp-5883	748	8	,	,	PUNCT
fnp-5883	748	9	t.s	t.s	PROPN
fnp-5883	748	10	.	.	PROPN
fnp-5883	748	11	,	,	PUNCT
fnp-5883	748	12	1983	1983	NUM
fnp-5883	748	13	.	.	PUNCT
fnp-5883	749	1	cytoplasmic	cytoplasmic	ADJ
fnp-5883	749	2	organization	organization	NOUN
fnp-5883	749	3	in	in	ADP
fnp-5883	749	4	cerebellar	cerebellar	ADJ
fnp-5883	749	5	dendritic	dendritic	ADJ
fnp-5883	749	6	spines	spine	NOUN
fnp-5883	749	7	.	.	PUNCT
fnp-5883	750	1	j.	j.	PROPN
fnp-5883	750	2	cell	cell	PROPN
fnp-5883	750	3	biol	biol	PROPN
fnp-5883	750	4	.	.	PUNCT
fnp-5883	751	1	97	97	NUM
fnp-5883	751	2	,	,	PUNCT
fnp-5883	751	3	1169–1178	1169–1178	NUM
fnp-5883	751	4	.	.	PUNCT
fnp-5883	752	1	https://doi.org/10.1083/jcb.97.4.1169	https://doi.org/10.1083/jcb.97.4.1169	PROPN
fnp-5883	752	2	landis	landis	PROPN
fnp-5883	752	3	,	,	PUNCT
fnp-5883	752	4	d.m	d.m	PROPN
fnp-5883	752	5	.	.	PROPN
fnp-5883	752	6	,	,	PUNCT
fnp-5883	752	7	reese	reese	PROPN
fnp-5883	752	8	,	,	PUNCT
fnp-5883	752	9	t.s	t.s	PROPN
fnp-5883	752	10	.	.	PROPN
fnp-5883	752	11	,	,	PUNCT
fnp-5883	752	12	1981	1981	NUM
fnp-5883	752	13	.	.	PUNCT
fnp-5883	753	1	astrocyte	astrocyte	NOUN
fnp-5883	753	2	membrane	membrane	NOUN
fnp-5883	753	3	structure	structure	NOUN
fnp-5883	753	4	:	:	PUNCT
fnp-5883	753	5	changes	change	NOUN
fnp-5883	753	6	after	after	ADP
fnp-5883	753	7	circulatory	circulatory	ADJ
fnp-5883	753	8	arrest	arrest	NOUN
fnp-5883	753	9	.	.	PUNCT
fnp-5883	754	1	j.	j.	PROPN
fnp-5883	754	2	cell	cell	PROPN
fnp-5883	754	3	biol	biol	PROPN
fnp-5883	754	4	.	.	PUNCT
fnp-5883	755	1	88	88	NUM
fnp-5883	755	2	,	,	PUNCT
fnp-5883	755	3	660–663	660–663	NUM
fnp-5883	755	4	.	.	PUNCT
fnp-5883	756	1	https://doi.org/10.1083/jcb.88.3.660	https://doi.org/10.1083/jcb.88.3.660	PROPN
fnp-5883	756	2	lee	lee	PROPN
fnp-5883	756	3	,	,	PUNCT
fnp-5883	756	4	e.	e.	PROPN
fnp-5883	756	5	,	,	PUNCT
fnp-5883	756	6	baiz	baiz	PROPN
fnp-5883	756	7	,	,	PUNCT
fnp-5883	756	8	c.r	c.r	PROPN
fnp-5883	756	9	.	.	PROPN
fnp-5883	756	10	,	,	PUNCT
fnp-5883	756	11	2022	2022	NUM
fnp-5883	756	12	.	.	PUNCT
fnp-5883	757	1	how	how	SCONJ
fnp-5883	757	2	cryoprotectants	cryoprotectant	NOUN
fnp-5883	757	3	work	work	VERB
fnp-5883	757	4	:	:	PUNCT
fnp-5883	757	5	hydrogen	hydrogen	NOUN
fnp-5883	757	6	-	-	PUNCT
fnp-5883	757	7	bonding	bonding	NOUN
fnp-5883	757	8	in	in	ADP
fnp-5883	757	9	low	low	ADJ
fnp-5883	757	10	-	-	PUNCT
fnp-5883	757	11	temperature	temperature	NOUN
fnp-5883	757	12	vitrified	vitrify	VERB
fnp-5883	757	13	solutions	solution	NOUN
fnp-5883	757	14	.	.	PUNCT
fnp-5883	758	1	chem	chem	NOUN
fnp-5883	758	2	.	.	PUNCT
fnp-5883	759	1	sci	sci	PROPN
fnp-5883	759	2	.	.	PROPN
fnp-5883	759	3	13	13	NUM
fnp-5883	759	4	,	,	PUNCT
fnp-5883	759	5	9980–9984	9980–9984	NUM
fnp-5883	759	6	.	.	PUNCT
fnp-5883	759	7	https://doi.org/10.1039/d2sc03188d	https://doi.org/10.1039/d2sc03188d	PROPN
fnp-5883	759	8	lee	lee	PROPN
fnp-5883	759	9	,	,	PUNCT
fnp-5883	759	10	r.e	r.e	PROPN
fnp-5883	759	11	.	.	PROPN
fnp-5883	759	12	,	,	PUNCT
fnp-5883	759	13	allenspach	allenspach	NOUN
fnp-5883	759	14	,	,	PUNCT
fnp-5883	759	15	a.l	a.l	PROPN
fnp-5883	759	16	.	.	PROPN
fnp-5883	759	17	,	,	PUNCT
fnp-5883	759	18	collins	collins	PROPN
fnp-5883	759	19	,	,	PUNCT
fnp-5883	759	20	s.d	s.d	PROPN
fnp-5883	759	21	.	.	PROPN
fnp-5883	759	22	,	,	PUNCT
fnp-5883	759	23	1997	1997	NUM
fnp-5883	759	24	.	.	PUNCT
fnp-5883	760	1	ultrastructural	ultrastructural	ADJ
fnp-5883	760	2	effects	effect	NOUN
fnp-5883	760	3	of	of	ADP
fnp-5883	760	4	lethal	lethal	ADJ
fnp-5883	760	5	freezing	freezing	NOUN
fnp-5883	760	6	on	on	ADP
fnp-5883	760	7	brain	brain	NOUN
fnp-5883	760	8	,	,	PUNCT
fnp-5883	760	9	muscle	muscle	NOUN
fnp-5883	760	10	and	and	CCONJ
fnp-5883	760	11	malpighian	malpighian	ADJ
fnp-5883	760	12	tubules	tubule	NOUN
fnp-5883	760	13	from	from	ADP
fnp-5883	760	14	freeze	freeze	ADJ
fnp-5883	760	15	-	-	PUNCT
fnp-5883	760	16	tolerant	tolerant	ADJ
fnp-5883	760	17	larvae	larvae	NOUN
fnp-5883	760	18	of	of	ADP
fnp-5883	760	19	the	the	DET
fnp-5883	760	20	gall	gall	NOUN
fnp-5883	760	21	fly	fly	NOUN
fnp-5883	760	22	,	,	PUNCT
fnp-5883	760	23	eurosta	eurosta	PROPN
fnp-5883	760	24	solidaginis	solidaginis	PROPN
fnp-5883	760	25	.	.	PUNCT
fnp-5883	761	1	j.	j.	PROPN
fnp-5883	761	2	insect	insect	PROPN
fnp-5883	761	3	physiol	physiol	PROPN
fnp-5883	761	4	.	.	PUNCT
fnp-5883	762	1	43	43	NUM
fnp-5883	762	2	,	,	PUNCT
fnp-5883	762	3	39–45	39–45	NUM
fnp-5883	762	4	.	.	PUNCT
fnp-5883	762	5	lemler	lemler	PROPN
fnp-5883	762	6	,	,	PUNCT
fnp-5883	762	7	j.	j.	PROPN
fnp-5883	762	8	,	,	PUNCT
fnp-5883	762	9	harris	harris	PROPN
fnp-5883	762	10	,	,	PUNCT
fnp-5883	762	11	s.b	s.b	PROPN
fnp-5883	762	12	.	.	PROPN
fnp-5883	762	13	,	,	PUNCT
fnp-5883	762	14	platt	platt	PROPN
fnp-5883	762	15	,	,	PUNCT
fnp-5883	762	16	c.	c.	PROPN
fnp-5883	762	17	,	,	PUNCT
fnp-5883	762	18	huffman	huffman	PROPN
fnp-5883	762	19	,	,	PUNCT
fnp-5883	762	20	t.m	t.m	PROPN
fnp-5883	762	21	.	.	PROPN
fnp-5883	762	22	,	,	PUNCT
fnp-5883	762	23	2004	2004	NUM
fnp-5883	762	24	.	.	PUNCT
fnp-5883	763	1	the	the	DET
fnp-5883	763	2	arrest	arrest	NOUN
fnp-5883	763	3	of	of	ADP
fnp-5883	763	4	biological	biological	ADJ
fnp-5883	763	5	time	time	NOUN
fnp-5883	763	6	as	as	ADP
fnp-5883	763	7	a	a	DET
fnp-5883	763	8	bridge	bridge	NOUN
fnp-5883	763	9	to	to	ADP
fnp-5883	763	10	engineered	engineer	VERB
fnp-5883	763	11	negligible	negligible	ADJ
fnp-5883	763	12	senescence	senescence	NOUN
fnp-5883	763	13	.	.	PUNCT
fnp-5883	764	1	ann	ann	PROPN
fnp-5883	764	2	.	.	PUNCT
fnp-5883	764	3	n.	n.	PROPN
fnp-5883	764	4	y.	y.	PROPN
fnp-5883	764	5	acad	acad	PROPN
fnp-5883	764	6	.	.	PUNCT
fnp-5883	765	1	sci	sci	PROPN
fnp-5883	765	2	.	.	PROPN
fnp-5883	766	1	1019	1019	NUM
fnp-5883	766	2	,	,	PUNCT
fnp-5883	766	3	559–563	559–563	NUM
fnp-5883	766	4	.	.	PUNCT
fnp-5883	767	1	https://doi.org/10.1196/annals.1297.104	https://doi.org/10.1196/annals.1297.104	PROPN
fnp-5883	767	2	leonard	leonard	PROPN
fnp-5883	767	3	,	,	PUNCT
fnp-5883	767	4	a.	a.	PROPN
fnp-5883	767	5	,	,	PUNCT
fnp-5883	767	6	vink	vink	PROPN
fnp-5883	767	7	,	,	PUNCT
fnp-5883	767	8	r.	r.	PROPN
fnp-5883	767	9	,	,	PUNCT
fnp-5883	767	10	byard	byard	ADV
fnp-5883	767	11	,	,	PUNCT
fnp-5883	767	12	r.w	r.w	PROPN
fnp-5883	767	13	.	.	PROPN
fnp-5883	767	14	,	,	PUNCT
fnp-5883	767	15	2016	2016	NUM
fnp-5883	767	16	.	.	PUNCT
fnp-5883	768	1	brain	brain	NOUN
fnp-5883	768	2	fluid	fluid	NOUN
fnp-5883	768	3	content	content	NOUN
fnp-5883	768	4	related	relate	VERB
fnp-5883	768	5	to	to	ADP
fnp-5883	768	6	body	body	NOUN
fnp-5883	768	7	position	position	NOUN
fnp-5883	768	8	and	and	CCONJ
fnp-5883	768	9	postmortem	postmortem	NOUN
fnp-5883	768	10	interval	interval	NOUN
fnp-5883	768	11	an	an	DET
fnp-5883	768	12	animal	animal	NOUN
fnp-5883	768	13	model	model	NOUN
fnp-5883	768	14	.	.	PUNCT
fnp-5883	769	1	j.	j.	PROPN
fnp-5883	769	2	forensic	forensic	PROPN
fnp-5883	769	3	sci	sci	PROPN
fnp-5883	769	4	.	.	PROPN
fnp-5883	769	5	61	61	NUM
fnp-5883	769	6	,	,	PUNCT
fnp-5883	769	7	671–673	671–673	PROPN
fnp-5883	769	8	.	.	PUNCT
fnp-5883	770	1	https://doi.org/10.1111/1556-4029.13038	https://doi.org/10.1111/1556-4029.13038	PROPN
fnp-5883	770	2	li	li	PROPN
fnp-5883	770	3	,	,	PUNCT
fnp-5883	770	4	j.z	j.z	PROPN
fnp-5883	770	5	.	.	PROPN
fnp-5883	770	6	,	,	PUNCT
fnp-5883	770	7	meng	meng	PROPN
fnp-5883	770	8	,	,	PUNCT
fnp-5883	770	9	f.	f.	PROPN
fnp-5883	770	10	,	,	PUNCT
fnp-5883	770	11	tsavaler	tsavaler	NOUN
fnp-5883	770	12	,	,	PUNCT
fnp-5883	770	13	l.	l.	PROPN
fnp-5883	770	14	,	,	PUNCT
fnp-5883	770	15	evans	evans	PROPN
fnp-5883	770	16	,	,	PUNCT
fnp-5883	770	17	s.j	s.j	PROPN
fnp-5883	770	18	.	.	PROPN
fnp-5883	770	19	,	,	PUNCT
fnp-5883	770	20	choudary	choudary	PROPN
fnp-5883	770	21	,	,	PUNCT
fnp-5883	770	22	p.v	p.v	PROPN
fnp-5883	770	23	.	.	PROPN
fnp-5883	770	24	,	,	PUNCT
fnp-5883	770	25	tomita	tomita	PROPN
fnp-5883	770	26	,	,	PUNCT
fnp-5883	770	27	h.	h.	PROPN
fnp-5883	770	28	,	,	PUNCT
fnp-5883	770	29	vawter	vawter	NOUN
fnp-5883	770	30	,	,	PUNCT
fnp-5883	770	31	m.p	m.p	PROPN
fnp-5883	770	32	.	.	PROPN
fnp-5883	770	33	,	,	PUNCT
fnp-5883	770	34	walsh	walsh	PROPN
fnp-5883	770	35	,	,	PUNCT
fnp-5883	770	36	d.	d.	PROPN
fnp-5883	770	37	,	,	PUNCT
fnp-5883	770	38	shokoohi	shokoohi	PROPN
fnp-5883	770	39	,	,	PUNCT
fnp-5883	770	40	v.	v.	PROPN
fnp-5883	770	41	,	,	PUNCT
fnp-5883	770	42	chung	chung	PROPN
fnp-5883	770	43	,	,	PUNCT
fnp-5883	770	44	t.	t.	PROPN
fnp-5883	770	45	,	,	PUNCT
fnp-5883	770	46	bunney	bunney	NOUN
fnp-5883	770	47	,	,	PUNCT
fnp-5883	770	48	w.e	w.e	PROPN
fnp-5883	770	49	.	.	PROPN
fnp-5883	770	50	,	,	PUNCT
fnp-5883	770	51	jones	jones	PROPN
fnp-5883	770	52	,	,	PUNCT
fnp-5883	770	53	e.g.	e.g.	ADV
fnp-5883	770	54	,	,	PUNCT
fnp-5883	770	55	akil	akil	PROPN
fnp-5883	770	56	,	,	PUNCT
fnp-5883	770	57	h.	h.	PROPN
fnp-5883	770	58	,	,	PUNCT
fnp-5883	770	59	watson	watson	PROPN
fnp-5883	770	60	,	,	PUNCT
fnp-5883	770	61	s.j	s.j	PROPN
fnp-5883	770	62	.	.	PROPN
fnp-5883	770	63	,	,	PUNCT
fnp-5883	770	64	myers	myers	PROPN
fnp-5883	770	65	,	,	PUNCT
fnp-5883	770	66	r.m	r.m	PROPN
fnp-5883	770	67	.	.	PROPN
fnp-5883	770	68	,	,	PUNCT
fnp-5883	770	69	2007	2007	NUM
fnp-5883	770	70	.	.	PUNCT
fnp-5883	771	1	sample	sample	NOUN
fnp-5883	771	2	matching	matching	NOUN
fnp-5883	771	3	by	by	ADP
fnp-5883	771	4	inferred	infer	VERB
fnp-5883	771	5	agonal	agonal	ADJ
fnp-5883	771	6	stress	stress	NOUN
fnp-5883	771	7	in	in	ADP
fnp-5883	771	8	gene	gene	NOUN
fnp-5883	771	9	expression	expression	NOUN
fnp-5883	771	10	analyses	analysis	NOUN
fnp-5883	771	11	of	of	ADP
fnp-5883	771	12	the	the	DET
fnp-5883	771	13	brain	brain	NOUN
fnp-5883	771	14	.	.	PUNCT
fnp-5883	772	1	bmc	bmc	ADJ
fnp-5883	772	2	genomics	genomic	NOUN
fnp-5883	772	3	8	8	NUM
fnp-5883	772	4	,	,	PUNCT
fnp-5883	772	5	336	336	NUM
fnp-5883	772	6	.	.	PUNCT
fnp-5883	773	1	https://doi.org/10.1186/1471-2164-8-336	https://doi.org/10.1186/1471-2164-8-336	PROPN
fnp-5883	773	2	li	li	PROPN
fnp-5883	773	3	,	,	PUNCT
fnp-5883	773	4	j.z	j.z	PROPN
fnp-5883	773	5	.	.	PROPN
fnp-5883	773	6	,	,	PUNCT
fnp-5883	773	7	vawter	vawter	NOUN
fnp-5883	773	8	,	,	PUNCT
fnp-5883	773	9	m.p	m.p	PROPN
fnp-5883	773	10	.	.	PROPN
fnp-5883	773	11	,	,	PUNCT
fnp-5883	773	12	walsh	walsh	PROPN
fnp-5883	773	13	,	,	PUNCT
fnp-5883	773	14	d.m	d.m	PROPN
fnp-5883	773	15	.	.	PROPN
fnp-5883	773	16	,	,	PUNCT
fnp-5883	773	17	tomita	tomita	PROPN
fnp-5883	773	18	,	,	PUNCT
fnp-5883	773	19	h.	h.	PROPN
fnp-5883	773	20	,	,	PUNCT
fnp-5883	773	21	evans	evans	PROPN
fnp-5883	773	22	,	,	PUNCT
fnp-5883	773	23	s.j	s.j	PROPN
fnp-5883	773	24	.	.	PROPN
fnp-5883	773	25	,	,	PUNCT
fnp-5883	773	26	choudary	choudary	PROPN
fnp-5883	773	27	,	,	PUNCT
fnp-5883	773	28	p.v	p.v	PROPN
fnp-5883	773	29	.	.	PROPN
fnp-5883	773	30	,	,	PUNCT
fnp-5883	773	31	lopez	lopez	PROPN
fnp-5883	773	32	,	,	PUNCT
fnp-5883	773	33	j.f	j.f	PROPN
fnp-5883	773	34	.	.	PROPN
fnp-5883	773	35	,	,	PUNCT
fnp-5883	773	36	avelar	avelar	PROPN
fnp-5883	773	37	,	,	PUNCT
fnp-5883	773	38	a.	a.	NOUN
fnp-5883	773	39	,	,	PUNCT
fnp-5883	773	40	shokoohi	shokoohi	PROPN
fnp-5883	773	41	,	,	PUNCT
fnp-5883	773	42	v.	v.	PROPN
fnp-5883	773	43	,	,	PUNCT
fnp-5883	773	44	chung	chung	PROPN
fnp-5883	773	45	,	,	PUNCT
fnp-5883	773	46	t.	t.	PROPN
fnp-5883	773	47	,	,	PUNCT
fnp-5883	773	48	mesarwi	mesarwi	NOUN
fnp-5883	773	49	,	,	PUNCT
fnp-5883	773	50	o.	o.	PROPN
fnp-5883	773	51	,	,	PUNCT
fnp-5883	773	52	jones	jones	PROPN
fnp-5883	773	53	,	,	PUNCT
fnp-5883	773	54	e.g.	e.g.	ADV
fnp-5883	773	55	,	,	PUNCT
fnp-5883	773	56	watson	watson	PROPN
fnp-5883	773	57	,	,	PUNCT
fnp-5883	773	58	s.j	s.j	PROPN
fnp-5883	773	59	.	.	PROPN
fnp-5883	773	60	,	,	PUNCT
fnp-5883	773	61	akil	akil	PROPN
fnp-5883	773	62	,	,	PUNCT
fnp-5883	773	63	h.	h.	PROPN
fnp-5883	773	64	,	,	PUNCT
fnp-5883	773	65	bunney	bunney	NOUN
fnp-5883	773	66	,	,	PUNCT
fnp-5883	773	67	w.e	w.e	PROPN
fnp-5883	773	68	.	.	PROPN
fnp-5883	773	69	,	,	PUNCT
fnp-5883	773	70	jr	jr	PROPN
fnp-5883	773	71	,	,	PUNCT
fnp-5883	773	72	myers	myers	PROPN
fnp-5883	773	73	,	,	PUNCT
fnp-5883	773	74	r.m	r.m	PROPN
fnp-5883	773	75	.	.	PROPN
fnp-5883	773	76	,	,	PUNCT
fnp-5883	773	77	2004	2004	NUM
fnp-5883	773	78	.	.	PUNCT
fnp-5883	774	1	systematic	systematic	ADJ
fnp-5883	774	2	changes	change	NOUN
fnp-5883	774	3	in	in	ADP
fnp-5883	774	4	gene	gene	NOUN
fnp-5883	774	5	expression	expression	NOUN
fnp-5883	774	6	in	in	ADP
fnp-5883	774	7	postmortem	postmortem	NOUN
fnp-5883	774	8	human	human	ADJ
fnp-5883	774	9	brains	brain	NOUN
fnp-5883	774	10	associated	associate	VERB
fnp-5883	774	11	with	with	ADP
fnp-5883	774	12	tissue	tissue	NOUN
fnp-5883	774	13	ph	ph	ADJ
fnp-5883	774	14	and	and	CCONJ
fnp-5883	774	15	terminal	terminal	ADJ
fnp-5883	774	16	medical	medical	ADJ
fnp-5883	774	17	conditions	condition	NOUN
fnp-5883	774	18	.	.	PUNCT
fnp-5883	775	1	hum	hum	ADJ
fnp-5883	775	2	.	.	PUNCT
fnp-5883	776	1	mol	mol	PROPN
fnp-5883	776	2	.	.	PUNCT
fnp-5883	777	1	genet	genet	NOUN
fnp-5883	777	2	.	.	PUNCT
fnp-5883	778	1	13	13	NUM
fnp-5883	778	2	,	,	PUNCT
fnp-5883	778	3	609–616	609–616	NUM
fnp-5883	778	4	.	.	PUNCT
fnp-5883	779	1	https://doi.org/10.1093/hmg/ddh065	https://doi.org/10.1093/hmg/ddh065	PROPN
fnp-5883	779	2	li	li	PROPN
fnp-5883	779	3	,	,	PUNCT
fnp-5883	779	4	w.	w.	PROPN
fnp-5883	779	5	,	,	PUNCT
fnp-5883	779	6	chen	chen	PROPN
fnp-5883	779	7	,	,	PUNCT
fnp-5883	779	8	q.	q.	PROPN
fnp-5883	779	9	,	,	PUNCT
fnp-5883	779	10	wang	wang	PROPN
fnp-5883	779	11	,	,	PUNCT
fnp-5883	779	12	x.	x.	PROPN
fnp-5883	779	13	,	,	PUNCT
fnp-5883	779	14	chen	chen	PROPN
fnp-5883	779	15	,	,	PUNCT
fnp-5883	779	16	z.	z.	PROPN
fnp-5883	779	17	,	,	PUNCT
fnp-5883	779	18	2023	2023	NUM
fnp-5883	779	19	.	.	PUNCT
fnp-5883	780	1	effect	effect	NOUN
fnp-5883	780	2	of	of	ADP
fnp-5883	780	3	freezing	freeze	VERB
fnp-5883	780	4	on	on	ADP
fnp-5883	780	5	soybean	soybean	NOUN
fnp-5883	780	6	protein	protein	NOUN
fnp-5883	780	7	solution	solution	NOUN
fnp-5883	780	8	.	.	PUNCT
fnp-5883	781	1	foods	food	NOUN
fnp-5883	781	2	basel	basel	PROPN
fnp-5883	781	3	switz	switz	PROPN
fnp-5883	781	4	.	.	PUNCT
fnp-5883	782	1	12	12	NUM
fnp-5883	782	2	,	,	PUNCT
fnp-5883	782	3	2650	2650	NUM
fnp-5883	782	4	.	.	PUNCT
fnp-5883	783	1	https://doi.org/10.3390/foods12142650	https://doi.org/10.3390/foods12142650	NOUN
fnp-5883	783	2	linner	linner	NOUN
fnp-5883	783	3	,	,	PUNCT
fnp-5883	783	4	j.g	j.g	PROPN
fnp-5883	783	5	.	.	PROPN
fnp-5883	783	6	,	,	PUNCT
fnp-5883	783	7	livesey	livesey	ADJ
fnp-5883	783	8	,	,	PUNCT
fnp-5883	783	9	s.a	s.a	PROPN
fnp-5883	783	10	.	.	PROPN
fnp-5883	783	11	,	,	PUNCT
fnp-5883	783	12	harrison	harrison	PROPN
fnp-5883	783	13	,	,	PUNCT
fnp-5883	783	14	d.s	d.s	PROPN
fnp-5883	783	15	.	.	PROPN
fnp-5883	783	16	,	,	PUNCT
fnp-5883	783	17	steiner	steiner	PROPN
fnp-5883	783	18	,	,	PUNCT
fnp-5883	783	19	a.l	a.l	PROPN
fnp-5883	783	20	.	.	PROPN
fnp-5883	783	21	,	,	PUNCT
fnp-5883	783	22	1986	1986	NUM
fnp-5883	783	23	.	.	PUNCT
fnp-5883	784	1	a	a	DET
fnp-5883	784	2	new	new	ADJ
fnp-5883	784	3	technique	technique	NOUN
fnp-5883	784	4	for	for	ADP
fnp-5883	784	5	removal	removal	NOUN
fnp-5883	784	6	of	of	ADP
fnp-5883	784	7	amorphous	amorphous	ADJ
fnp-5883	784	8	phase	phase	NOUN
fnp-5883	784	9	tissue	tissue	NOUN
fnp-5883	784	10	water	water	NOUN
fnp-5883	784	11	without	without	ADP
fnp-5883	784	12	ice	ice	NOUN
fnp-5883	784	13	crystal	crystal	NOUN
fnp-5883	784	14	damage	damage	NOUN
fnp-5883	784	15	:	:	PUNCT
fnp-5883	784	16	a	a	DET
fnp-5883	784	17	preparative	preparative	ADJ
fnp-5883	784	18	method	method	NOUN
fnp-5883	784	19	for	for	ADP
fnp-5883	784	20	ultrastructural	ultrastructural	ADJ
fnp-5883	784	21	analysis	analysis	NOUN
fnp-5883	784	22	and	and	CCONJ
fnp-5883	784	23	immunoelectron	immunoelectron	ADJ
fnp-5883	784	24	microscopy	microscopy	NOUN
fnp-5883	784	25	.	.	PUNCT
fnp-5883	785	1	j.	j.	PROPN
fnp-5883	785	2	histochem	histochem	PROPN
fnp-5883	785	3	.	.	PUNCT
fnp-5883	786	1	cytochem	cytochem	PROPN
fnp-5883	786	2	.	.	PUNCT
fnp-5883	787	1	off	off	ADV
fnp-5883	787	2	.	.	PUNCT
fnp-5883	788	1	j.	j.	PROPN
fnp-5883	788	2	histochem	histochem	PROPN
fnp-5883	788	3	.	.	PUNCT
fnp-5883	789	1	soc	soc	PROPN
fnp-5883	789	2	.	.	PUNCT
fnp-5883	790	1	34	34	NUM
fnp-5883	790	2	,	,	PUNCT
fnp-5883	790	3	1123–1135	1123–1135	NUM
fnp-5883	790	4	.	.	PUNCT
fnp-5883	791	1	https://doi.org/10.1177/34.9.2426340	https://doi.org/10.1177/34.9.2426340	PROPN
fnp-5883	791	2	marshall	marshall	PROPN
fnp-5883	791	3	,	,	PUNCT
fnp-5883	791	4	w.h	w.h	PROPN
fnp-5883	791	5	.	.	PROPN
fnp-5883	791	6	,	,	PUNCT
fnp-5883	791	7	1940	1940	NUM
fnp-5883	791	8	.	.	PUNCT
fnp-5883	792	1	an	an	DET
fnp-5883	792	2	application	application	NOUN
fnp-5883	792	3	of	of	ADP
fnp-5883	792	4	the	the	DET
fnp-5883	792	5	frozen	frozen	ADJ
fnp-5883	792	6	sectioning	section	VERB
fnp-5883	792	7	technic	technic	NOUN
fnp-5883	792	8	for	for	ADP
fnp-5883	792	9	cutting	cut	VERB
fnp-5883	792	10	serial	serial	ADJ
fnp-5883	792	11	sections	section	NOUN
fnp-5883	792	12	thru	thru	ADP
fnp-5883	792	13	the	the	DET
fnp-5883	792	14	brain	brain	NOUN
fnp-5883	792	15	.	.	PUNCT
fnp-5883	793	1	stain	stain	NOUN
fnp-5883	793	2	technol	technol	NOUN
fnp-5883	793	3	.	.	PROPN
fnp-5883	793	4	15	15	NUM
fnp-5883	793	5	,	,	PUNCT
fnp-5883	793	6	133–138	133–138	NUM
fnp-5883	793	7	.	.	PUNCT
fnp-5883	794	1	https://doi.org/10.3109/10520294009110338	https://doi.org/10.3109/10520294009110338	PROPN
fnp-5883	794	2	massie	massie	PROPN
fnp-5883	794	3	,	,	PUNCT
fnp-5883	794	4	i.	i.	PROPN
fnp-5883	794	5	,	,	PUNCT
fnp-5883	794	6	selden	selden	PROPN
fnp-5883	794	7	,	,	PUNCT
fnp-5883	794	8	c.	c.	PROPN
fnp-5883	794	9	,	,	PUNCT
fnp-5883	794	10	hodgson	hodgson	PROPN
fnp-5883	794	11	,	,	PUNCT
fnp-5883	794	12	h.	h.	PROPN
fnp-5883	794	13	,	,	PUNCT
fnp-5883	794	14	fuller	full	ADJ
fnp-5883	794	15	,	,	PUNCT
fnp-5883	794	16	b.	b.	PROPN
fnp-5883	794	17	,	,	PUNCT
fnp-5883	794	18	2013	2013	NUM
fnp-5883	794	19	.	.	PUNCT
fnp-5883	795	1	storage	storage	NOUN
fnp-5883	795	2	temperatures	temperature	NOUN
fnp-5883	795	3	for	for	ADP
fnp-5883	795	4	cold	cold	ADJ
fnp-5883	795	5	-	-	PUNCT
fnp-5883	795	6	chain	chain	NOUN
fnp-5883	795	7	delivery	delivery	NOUN
fnp-5883	795	8	in	in	ADP
fnp-5883	795	9	cell	cell	NOUN
fnp-5883	795	10	therapy	therapy	NOUN
fnp-5883	795	11	:	:	PUNCT
fnp-5883	795	12	a	a	DET
fnp-5883	795	13	study	study	NOUN
fnp-5883	795	14	of	of	ADP
fnp-5883	795	15	alginate	alginate	NOUN
fnp-5883	795	16	-	-	PUNCT
fnp-5883	795	17	encapsulated	encapsulate	VERB
fnp-5883	795	18	liver	liver	NOUN
fnp-5883	795	19	cell	cell	NOUN
fnp-5883	795	20	spheroids	spheroid	NOUN
fnp-5883	795	21	stored	store	VERB
fnp-5883	795	22	at	at	ADP
fnp-5883	795	23	-80	-80	PUNCT
fnp-5883	795	24	 	 	SPACE
fnp-5883	795	25	°	°	ADP
fnp-5883	795	26	c	c	NOUN
fnp-5883	795	27	or	or	CCONJ
fnp-5883	795	28	-170	-170	NOUN
fnp-5883	795	29	 	 	SPACE
fnp-5883	795	30	°	°	NOUN
fnp-5883	795	31	c	c	NOUN
fnp-5883	795	32	for	for	ADP
fnp-5883	795	33	up	up	ADP
fnp-5883	795	34	to	to	PART
fnp-5883	795	35	1	1	NUM
fnp-5883	795	36	year	year	NOUN
fnp-5883	795	37	.	.	PUNCT
fnp-5883	796	1	tissue	tissue	NOUN
fnp-5883	796	2	eng	eng	PROPN
fnp-5883	796	3	.	.	PUNCT
fnp-5883	797	1	part	part	NOUN
fnp-5883	797	2	c	c	PROPN
fnp-5883	797	3	methods	method	NOUN
fnp-5883	797	4	19	19	NUM
fnp-5883	797	5	,	,	PUNCT
fnp-5883	797	6	189–195	189–195	NUM
fnp-5883	797	7	.	.	PUNCT
fnp-5883	798	1	https://doi.org/10.1089/ten.tec.2012.0307	https://doi.org/10.1089/ten.tec.2012.0307	NOUN
fnp-5883	798	2	mazur	mazur	PROPN
fnp-5883	798	3	,	,	PUNCT
fnp-5883	798	4	p.	p.	PROPN
fnp-5883	798	5	,	,	PUNCT
fnp-5883	798	6	leibo	leibo	PROPN
fnp-5883	798	7	,	,	PUNCT
fnp-5883	798	8	s.p	s.p	PROPN
fnp-5883	798	9	.	.	PROPN
fnp-5883	798	10	,	,	PUNCT
fnp-5883	798	11	chu	chu	PROPN
fnp-5883	798	12	,	,	PUNCT
fnp-5883	798	13	e.h	e.h	PROPN
fnp-5883	798	14	.	.	PROPN
fnp-5883	798	15	,	,	PUNCT
fnp-5883	798	16	1972	1972	NUM
fnp-5883	798	17	.	.	PUNCT
fnp-5883	799	1	a	a	DET
fnp-5883	799	2	two	two	NUM
fnp-5883	799	3	-	-	PUNCT
fnp-5883	799	4	factor	factor	NOUN
fnp-5883	799	5	hypothesis	hypothesis	NOUN
fnp-5883	799	6	of	of	ADP
fnp-5883	799	7	freezing	freeze	VERB
fnp-5883	799	8	injury	injury	NOUN
fnp-5883	799	9	.	.	PUNCT
fnp-5883	800	1	evidence	evidence	NOUN
fnp-5883	800	2	from	from	ADP
fnp-5883	800	3	chinese	chinese	PROPN
fnp-5883	800	4	hamster	hamster	PROPN
fnp-5883	800	5	tissue	tissue	NOUN
fnp-5883	800	6	-	-	PUNCT
fnp-5883	800	7	culture	culture	NOUN
fnp-5883	800	8	cells	cell	NOUN
fnp-5883	800	9	.	.	PUNCT
fnp-5883	801	1	exp	exp	NOUN
fnp-5883	801	2	.	.	PUNCT
fnp-5883	801	3	cell	cell	NOUN
fnp-5883	801	4	res	re	NOUN
fnp-5883	801	5	.	.	PUNCT
fnp-5883	802	1	71	71	NUM
fnp-5883	802	2	,	,	PUNCT
fnp-5883	802	3	345–355	345–355	NUM
fnp-5883	802	4	.	.	PUNCT
fnp-5883	803	1	https://doi.org/10.1016/0014-4827(72)90303-5	https://doi.org/10.1016/0014-4827(72)90303-5	PROPN
fnp-5883	803	2	mcfadden	mcfadden	PROPN
fnp-5883	803	3	,	,	PUNCT
fnp-5883	803	4	w.c	w.c	PROPN
fnp-5883	803	5	.	.	PROPN
fnp-5883	803	6	,	,	PUNCT
fnp-5883	803	7	walsh	walsh	PROPN
fnp-5883	803	8	,	,	PUNCT
fnp-5883	803	9	h.	h.	PROPN
fnp-5883	803	10	,	,	PUNCT
fnp-5883	803	11	richter	richter	PROPN
fnp-5883	803	12	,	,	PUNCT
fnp-5883	803	13	f.	f.	PROPN
fnp-5883	803	14	,	,	PUNCT
fnp-5883	803	15	soudant	soudant	NOUN
fnp-5883	803	16	,	,	PUNCT
fnp-5883	803	17	c.	c.	PROPN
fnp-5883	803	18	,	,	PUNCT
fnp-5883	803	19	bryce	bryce	PROPN
fnp-5883	803	20	,	,	PUNCT
fnp-5883	803	21	c.h	c.h	PROPN
fnp-5883	803	22	.	.	PROPN
fnp-5883	803	23	,	,	PUNCT
fnp-5883	803	24	hof	hof	PROPN
fnp-5883	803	25	,	,	PUNCT
fnp-5883	803	26	p.r	p.r	PROPN
fnp-5883	803	27	.	.	PROPN
fnp-5883	803	28	,	,	PUNCT
fnp-5883	803	29	fowkes	fowke	NOUN
fnp-5883	803	30	,	,	PUNCT
fnp-5883	803	31	m.	m.	NOUN
fnp-5883	803	32	,	,	PUNCT
fnp-5883	803	33	crary	crary	NOUN
fnp-5883	803	34	,	,	PUNCT
fnp-5883	803	35	j.f	j.f	PROPN
fnp-5883	803	36	.	.	PROPN
fnp-5883	803	37	,	,	PUNCT
fnp-5883	803	38	mckenzie	mckenzie	PROPN
fnp-5883	803	39	,	,	PUNCT
fnp-5883	803	40	a.t	a.t	PROPN
fnp-5883	803	41	.	.	PROPN
fnp-5883	803	42	,	,	PUNCT
fnp-5883	803	43	2019	2019	NUM
fnp-5883	803	44	.	.	PUNCT
fnp-5883	804	1	perfusion	perfusion	NOUN
fnp-5883	804	2	fixation	fixation	NOUN
fnp-5883	804	3	in	in	ADP
fnp-5883	804	4	brain	brain	NOUN
fnp-5883	804	5	banking	banking	NOUN
fnp-5883	804	6	:	:	PUNCT
fnp-5883	804	7	a	a	DET
fnp-5883	804	8	systematic	systematic	ADJ
fnp-5883	804	9	review	review	NOUN
fnp-5883	804	10	.	.	PUNCT
fnp-5883	805	1	acta	acta	PROPN
fnp-5883	805	2	neuropathol	neuropathol	PROPN
fnp-5883	805	3	.	.	PUNCT
fnp-5883	806	1	commun	commun	PROPN
fnp-5883	806	2	.	.	PUNCT
fnp-5883	807	1	7	7	NUM
fnp-5883	807	2	,	,	PUNCT
fnp-5883	807	3	146	146	NUM
fnp-5883	807	4	.	.	PUNCT
fnp-5883	808	1	https://doi.org/10.1186/s40478-019-0799-y	https://doi.org/10.1186/s40478-019-0799-y	PROPN
fnp-5883	808	2	mcgrath	mcgrath	PROPN
fnp-5883	808	3	,	,	PUNCT
fnp-5883	808	4	j.j	j.j	PROPN
fnp-5883	808	5	.	.	PROPN
fnp-5883	808	6	,	,	PUNCT
fnp-5883	808	7	1985	1985	NUM
fnp-5883	808	8	.	.	PUNCT
fnp-5883	809	1	preservation	preservation	NOUN
fnp-5883	809	2	of	of	ADP
fnp-5883	809	3	biological	biological	ADJ
fnp-5883	809	4	material	material	NOUN
fnp-5883	809	5	by	by	ADP
fnp-5883	809	6	freezing	freeze	VERB
fnp-5883	809	7	and	and	CCONJ
fnp-5883	809	8	thawing	thawing	NOUN
fnp-5883	809	9	,	,	PUNCT
fnp-5883	809	10	in	in	ADP
fnp-5883	809	11	:	:	PUNCT
fnp-5883	809	12	shitzer	shitzer	PROPN
fnp-5883	809	13	,	,	PUNCT
fnp-5883	809	14	a.	a.	NOUN
fnp-5883	809	15	,	,	PUNCT
fnp-5883	809	16	eberhart	eberhart	PROPN
fnp-5883	809	17	,	,	PUNCT
fnp-5883	809	18	r.c	r.c	PROPN
fnp-5883	809	19	.	.	PROPN
fnp-5883	809	20	(	(	PUNCT
fnp-5883	809	21	eds	ed	NOUN
fnp-5883	809	22	.	.	PUNCT
fnp-5883	809	23	)	)	PUNCT
fnp-5883	809	24	,	,	PUNCT
fnp-5883	809	25	heat	heat	NOUN
fnp-5883	809	26	transfer	transfer	NOUN
fnp-5883	809	27	in	in	ADP
fnp-5883	809	28	medicine	medicine	NOUN
fnp-5883	809	29	and	and	CCONJ
fnp-5883	809	30	biology	biology	NOUN
fnp-5883	809	31	:	:	PUNCT
fnp-5883	809	32	analysis	analysis	NOUN
fnp-5883	809	33	and	and	CCONJ
fnp-5883	809	34	applications	application	NOUN
fnp-5883	809	35	.	.	PUNCT
fnp-5883	810	1	volume	volume	NOUN
fnp-5883	810	2	2	2	NUM
fnp-5883	810	3	.	.	PUNCT
fnp-5883	811	1	springer	springer	PROPN
fnp-5883	811	2	us	us	PROPN
fnp-5883	811	3	,	,	PUNCT
fnp-5883	811	4	boston	boston	PROPN
fnp-5883	811	5	,	,	PUNCT
fnp-5883	811	6	ma	ma	PROPN
fnp-5883	811	7	,	,	PUNCT
fnp-5883	811	8	pp	pp	ADJ
fnp-5883	811	9	.	.	PUNCT
fnp-5883	812	1	185–238	185–238	NUM
fnp-5883	812	2	.	.	PUNCT
fnp-5883	813	1	https://doi.org/10.1007/978-1-4684-8285-0_5	https://doi.org/10.1007/978-1-4684-8285-0_5	PROPN
fnp-5883	813	2	mcintyre	mcintyre	PROPN
fnp-5883	813	3	,	,	PUNCT
fnp-5883	813	4	j.a	j.a	PROPN
fnp-5883	813	5	.	.	PROPN
fnp-5883	813	6	,	,	PUNCT
fnp-5883	813	7	gilula	gilula	PROPN
fnp-5883	813	8	,	,	PUNCT
fnp-5883	813	9	n.b	n.b	PROPN
fnp-5883	813	10	.	.	PROPN
fnp-5883	813	11	,	,	PUNCT
fnp-5883	813	12	karnovsky	karnovsky	PROPN
fnp-5883	813	13	,	,	PUNCT
fnp-5883	813	14	m.j	m.j	PROPN
fnp-5883	813	15	.	.	PROPN
fnp-5883	813	16	,	,	PUNCT
fnp-5883	813	17	1974	1974	NUM
fnp-5883	813	18	.	.	PUNCT
fnp-5883	814	1	cryoprotectant	cryoprotectant	NOUN
fnp-5883	814	2	-	-	PUNCT
fnp-5883	814	3	induced	induce	VERB
fnp-5883	814	4	redistribution	redistribution	NOUN
fnp-5883	814	5	of	of	ADP
fnp-5883	814	6	intramembranous	intramembranous	ADJ
fnp-5883	814	7	particles	particle	NOUN
fnp-5883	814	8	in	in	ADP
fnp-5883	814	9	mouse	mouse	NOUN
fnp-5883	814	10	lymphocytes	lymphocyte	NOUN
fnp-5883	814	11	.	.	PUNCT
fnp-5883	815	1	j.	j.	PROPN
fnp-5883	815	2	cell	cell	PROPN
fnp-5883	815	3	biol	biol	PROPN
fnp-5883	815	4	.	.	PUNCT
fnp-5883	816	1	60	60	NUM
fnp-5883	816	2	,	,	PUNCT
fnp-5883	816	3	192–203	192–203	NUM
fnp-5883	816	4	.	.	PUNCT
fnp-5883	817	1	https://doi.org/10.1083/jcb.60.1.192	https://doi.org/10.1083/jcb.60.1.192	PROPN
fnp-5883	817	2	mcintyre	mcintyre	PROPN
fnp-5883	817	3	,	,	PUNCT
fnp-5883	817	4	r.l	r.l	PROPN
fnp-5883	817	5	.	.	PROPN
fnp-5883	817	6	,	,	PUNCT
fnp-5883	817	7	fahy	fahy	PROPN
fnp-5883	817	8	,	,	PUNCT
fnp-5883	817	9	g.m	g.m	PROPN
fnp-5883	817	10	.	.	PROPN
fnp-5883	817	11	,	,	PUNCT
fnp-5883	817	12	2015	2015	NUM
fnp-5883	817	13	.	.	PUNCT
fnp-5883	818	1	aldehyde	aldehyde	NOUN
fnp-5883	818	2	-	-	PUNCT
fnp-5883	818	3	stabilized	stabilize	VERB
fnp-5883	818	4	cryopreservation	cryopreservation	NOUN
fnp-5883	818	5	.	.	PUNCT
fnp-5883	819	1	cryobiology	cryobiology	NOUN
fnp-5883	819	2	71	71	NUM
fnp-5883	819	3	,	,	PUNCT
fnp-5883	819	4	448–458	448–458	NUM
fnp-5883	819	5	.	.	PUNCT
fnp-5883	820	1	https://doi.org/10.1016/j.cryobiol.2015.09.003	https://doi.org/10.1016/j.cryobiol.2015.09.003	PROPN
fnp-5883	820	2	mckee	mckee	PROPN
fnp-5883	820	3	,	,	PUNCT
fnp-5883	820	4	a.c	a.c	PROPN
fnp-5883	820	5	.	.	PROPN
fnp-5883	820	6	,	,	PUNCT
fnp-5883	820	7	1999	1999	NUM
fnp-5883	820	8	.	.	PUNCT
fnp-5883	821	1	brain	brain	NOUN
fnp-5883	821	2	banking	banking	NOUN
fnp-5883	821	3	:	:	PUNCT
fnp-5883	821	4	basic	basic	ADJ
fnp-5883	821	5	science	science	NOUN
fnp-5883	821	6	methods	method	NOUN
fnp-5883	821	7	.	.	PUNCT
fnp-5883	822	1	alzheimer	alzheimer	PROPN
fnp-5883	822	2	dis	dis	PROPN
fnp-5883	822	3	.	.	PUNCT
fnp-5883	823	1	assoc	assoc	PROPN
fnp-5883	823	2	.	.	PUNCT
fnp-5883	824	1	disord	disord	PROPN
fnp-5883	824	2	.	.	PUNCT
fnp-5883	825	1	13	13	NUM
fnp-5883	825	2	suppl	suppl	ADJ
fnp-5883	825	3	1	1	NUM
fnp-5883	825	4	,	,	PUNCT
fnp-5883	825	5	s39	s39	NOUN
fnp-5883	825	6	-	-	SYM
fnp-5883	825	7	44	44	NUM
fnp-5883	825	8	.	.	PUNCT
fnp-5883	825	9	mckenzie	mckenzie	PROPN
fnp-5883	825	10	,	,	PUNCT
fnp-5883	825	11	a.t	a.t	PROPN
fnp-5883	825	12	.	.	PROPN
fnp-5883	825	13	,	,	PUNCT
fnp-5883	825	14	woodoff	woodoff	NOUN
fnp-5883	825	15	-	-	PUNCT
fnp-5883	825	16	leith	leith	PROPN
fnp-5883	825	17	,	,	PUNCT
fnp-5883	825	18	e.	e.	PROPN
fnp-5883	825	19	,	,	PUNCT
fnp-5883	825	20	dangoor	dangoor	PROPN
fnp-5883	825	21	,	,	PUNCT
fnp-5883	825	22	d.	d.	PROPN
fnp-5883	825	23	,	,	PUNCT
fnp-5883	825	24	cervera	cervera	NOUN
fnp-5883	825	25	,	,	PUNCT
fnp-5883	825	26	a.	a.	NOUN
fnp-5883	825	27	,	,	PUNCT
fnp-5883	825	28	ressler	ressler	NOUN
fnp-5883	825	29	,	,	PUNCT
fnp-5883	825	30	h.	h.	PROPN
fnp-5883	825	31	,	,	PUNCT
fnp-5883	825	32	whitney	whitney	PROPN
fnp-5883	825	33	,	,	PUNCT
fnp-5883	825	34	k.	k.	PROPN
fnp-5883	825	35	,	,	PUNCT
fnp-5883	825	36	dams	dam	NOUN
fnp-5883	825	37	-	-	PUNCT
fnp-5883	825	38	o’connor	o’connor	ADV
fnp-5883	825	39	,	,	PUNCT
fnp-5883	825	40	k.	k.	PROPN
fnp-5883	825	41	,	,	PUNCT
fnp-5883	825	42	wu	wu	PROPN
fnp-5883	825	43	,	,	PUNCT
fnp-5883	825	44	z.	z.	PROPN
fnp-5883	825	45	,	,	PUNCT
fnp-5883	825	46	hillman	hillman	PROPN
fnp-5883	825	47	,	,	PUNCT
fnp-5883	825	48	e.m.c	e.m.c	PROPN
fnp-5883	825	49	.	.	PROPN
fnp-5883	825	50	,	,	PUNCT
fnp-5883	825	51	seifert	seifert	PROPN
fnp-5883	825	52	,	,	PUNCT
fnp-5883	825	53	a.c	a.c	PROPN
fnp-5883	825	54	.	.	PROPN
fnp-5883	825	55	,	,	PUNCT
fnp-5883	825	56	crary	crary	PROPN
fnp-5883	825	57	,	,	PUNCT
fnp-5883	825	58	j.f	j.f	PROPN
fnp-5883	825	59	.	.	PROPN
fnp-5883	825	60	,	,	PUNCT
fnp-5883	825	61	2022	2022	NUM
fnp-5883	825	62	.	.	PUNCT
fnp-5883	826	1	ex	ex	PRON
fnp-5883	826	2	situ	situ	ADJ
fnp-5883	826	3	perfusion	perfusion	NOUN
fnp-5883	826	4	fixation	fixation	NOUN
fnp-5883	826	5	for	for	ADP
fnp-5883	826	6	brain	brain	NOUN
fnp-5883	826	7	banking	banking	NOUN
fnp-5883	826	8	:	:	PUNCT
fnp-5883	826	9	a	a	DET
fnp-5883	826	10	technical	technical	ADJ
fnp-5883	826	11	report	report	NOUN
fnp-5883	826	12	.	.	PUNCT
fnp-5883	827	1	free	free	ADJ
fnp-5883	827	2	neuropathol	neuropathol	NOUN
fnp-5883	827	3	.	.	PUNCT
fnp-5883	828	1	3	3	NUM
fnp-5883	828	2	,	,	PUNCT
fnp-5883	828	3	22–22	22–22	NUM
fnp-5883	828	4	.	.	PUNCT
fnp-5883	828	5	https://doi.org/10.17879/freeneuropathology-2022-4368	https://doi.org/10.17879/freeneuropathology-2022-4368	PROPN
fnp-5883	828	6	meissner	meissner	PROPN
fnp-5883	828	7	,	,	PUNCT
fnp-5883	828	8	d.h	d.h	PROPN
fnp-5883	828	9	.	.	PROPN
fnp-5883	828	10	,	,	PUNCT
fnp-5883	828	11	schwarz	schwarz	PROPN
fnp-5883	828	12	,	,	PUNCT
fnp-5883	828	13	h.	h.	PROPN
fnp-5883	828	14	,	,	PUNCT
fnp-5883	828	15	1990	1990	NUM
fnp-5883	828	16	.	.	PUNCT
fnp-5883	829	1	improved	improve	VERB
fnp-5883	829	2	cryoprotection	cryoprotection	NOUN
fnp-5883	829	3	and	and	CCONJ
fnp-5883	829	4	freeze	freeze	NOUN
fnp-5883	829	5	-	-	PUNCT
fnp-5883	829	6	substitution	substitution	NOUN
fnp-5883	829	7	of	of	ADP
fnp-5883	829	8	embryonic	embryonic	ADJ
fnp-5883	829	9	quail	quail	NOUN
fnp-5883	829	10	retina	retina	NOUN
fnp-5883	829	11	:	:	PUNCT
fnp-5883	829	12	a	a	DET
fnp-5883	829	13	tem	tem	PROPN
fnp-5883	829	14	study	study	NOUN
fnp-5883	829	15	on	on	ADP
fnp-5883	829	16	ultrastructural	ultrastructural	ADJ
fnp-5883	829	17	preservation	preservation	NOUN
fnp-5883	829	18	.	.	PUNCT
fnp-5883	830	1	j.	j.	PROPN
fnp-5883	830	2	electron	electron	PROPN
fnp-5883	830	3	microsc	microsc	PROPN
fnp-5883	830	4	.	.	PUNCT
fnp-5883	831	1	tech	tech	NOUN
fnp-5883	831	2	.	.	PUNCT
fnp-5883	832	1	14	14	NUM
fnp-5883	832	2	,	,	PUNCT
fnp-5883	832	3	348–356	348–356	NUM
fnp-5883	832	4	.	.	PUNCT
fnp-5883	832	5	https://doi.org/10.1002/jemt.1060140410	https://doi.org/10.1002/jemt.1060140410	PROPN
fnp-5883	832	6	meneghel	meneghel	PROPN
fnp-5883	832	7	,	,	PUNCT
fnp-5883	832	8	j.	j.	PROPN
fnp-5883	832	9	,	,	PUNCT
fnp-5883	832	10	kilbride	kilbride	PROPN
fnp-5883	832	11	,	,	PUNCT
fnp-5883	832	12	p.	p.	PROPN
fnp-5883	832	13	,	,	PUNCT
fnp-5883	832	14	morris	morris	PROPN
fnp-5883	832	15	,	,	PUNCT
fnp-5883	832	16	j.g	j.g	PROPN
fnp-5883	832	17	.	.	PROPN
fnp-5883	832	18	,	,	PUNCT
fnp-5883	832	19	fonseca	fonseca	PROPN
fnp-5883	832	20	,	,	PUNCT
fnp-5883	832	21	f.	f.	PROPN
fnp-5883	832	22	,	,	PUNCT
fnp-5883	832	23	2019	2019	NUM
fnp-5883	832	24	.	.	PUNCT
fnp-5883	833	1	physical	physical	ADJ
fnp-5883	833	2	events	event	NOUN
fnp-5883	833	3	occurring	occur	VERB
fnp-5883	833	4	during	during	ADP
fnp-5883	833	5	the	the	DET
fnp-5883	833	6	cryopreservation	cryopreservation	NOUN
fnp-5883	833	7	of	of	ADP
fnp-5883	833	8	immortalized	immortalize	VERB
fnp-5883	833	9	human	human	PROPN
fnp-5883	833	10	t	t	NOUN
fnp-5883	833	11	cells	cell	NOUN
fnp-5883	833	12	.	.	PUNCT
fnp-5883	834	1	plos	plos	PROPN
fnp-5883	834	2	one	one	NUM
fnp-5883	834	3	14	14	NUM
fnp-5883	834	4	,	,	PUNCT
fnp-5883	834	5	e0217304	e0217304	PROPN
fnp-5883	834	6	.	.	PUNCT
fnp-5883	835	1	https://doi.org/10.1371/journal.pone.0217304	https://doi.org/10.1371/journal.pone.0217304	PROPN
fnp-5883	835	2	menz	menz	PROPN
fnp-5883	835	3	,	,	PUNCT
fnp-5883	835	4	l.j	l.j	PROPN
fnp-5883	835	5	.	.	PROPN
fnp-5883	835	6	,	,	PUNCT
fnp-5883	835	7	1971	1971	NUM
fnp-5883	835	8	.	.	PUNCT
fnp-5883	836	1	structural	structural	ADJ
fnp-5883	836	2	changes	change	NOUN
fnp-5883	836	3	and	and	CCONJ
fnp-5883	836	4	impairment	impairment	NOUN
fnp-5883	836	5	of	of	ADP
fnp-5883	836	6	function	function	NOUN
fnp-5883	836	7	associated	associate	VERB
fnp-5883	836	8	with	with	ADP
fnp-5883	836	9	freezing	freeze	VERB
fnp-5883	836	10	and	and	CCONJ
fnp-5883	836	11	thawing	thaw	VERB
fnp-5883	836	12	in	in	ADP
fnp-5883	836	13	muscle	muscle	NOUN
fnp-5883	836	14	,	,	PUNCT
fnp-5883	836	15	nerve	nerve	NOUN
fnp-5883	836	16	,	,	PUNCT
fnp-5883	836	17	and	and	CCONJ
fnp-5883	836	18	leucocytes	leucocyte	NOUN
fnp-5883	836	19	.	.	PUNCT
fnp-5883	837	1	cryobiology	cryobiology	NOUN
fnp-5883	837	2	8	8	NUM
fnp-5883	837	3	,	,	PUNCT
fnp-5883	837	4	1–13	1–13	NOUN
fnp-5883	837	5	.	.	PUNCT
fnp-5883	838	1	https://doi.org/10.1016/0011-2240(71)90102-7	https://doi.org/10.1016/0011-2240(71)90102-7	PROPN
fnp-5883	838	2	meyronet	meyronet	PROPN
fnp-5883	838	3	,	,	PUNCT
fnp-5883	838	4	d.	d.	PROPN
fnp-5883	838	5	,	,	PUNCT
fnp-5883	838	6	dorey	dorey	PROPN
fnp-5883	838	7	,	,	PUNCT
fnp-5883	838	8	a.	a.	PROPN
fnp-5883	838	9	,	,	PUNCT
fnp-5883	838	10	massoma	massoma	NOUN
fnp-5883	838	11	,	,	PUNCT
fnp-5883	838	12	p.	p.	PROPN
fnp-5883	838	13	,	,	PUNCT
fnp-5883	838	14	rey	rey	PROPN
fnp-5883	838	15	,	,	PUNCT
fnp-5883	838	16	c.	c.	PROPN
fnp-5883	838	17	,	,	PUNCT
fnp-5883	838	18	alix	alix	PROPN
fnp-5883	838	19	,	,	PUNCT
fnp-5883	838	20	e.	e.	PROPN
fnp-5883	838	21	,	,	PUNCT
fnp-5883	838	22	silva	silva	PROPN
fnp-5883	838	23	,	,	PUNCT
fnp-5883	838	24	k.	k.	PROPN
fnp-5883	838	25	,	,	PUNCT
fnp-5883	838	26	perrin	perrin	PROPN
fnp-5883	838	27	,	,	PUNCT
fnp-5883	838	28	c.	c.	PROPN
fnp-5883	838	29	,	,	PUNCT
fnp-5883	838	30	quadrio	quadrio	PROPN
fnp-5883	838	31	,	,	PUNCT
fnp-5883	838	32	i.	i.	PROPN
fnp-5883	838	33	,	,	PUNCT
fnp-5883	838	34	perret	perret	PROPN
fnp-5883	838	35	-	-	PUNCT
fnp-5883	838	36	liaudet	liaudet	NOUN
fnp-5883	838	37	,	,	PUNCT
fnp-5883	838	38	a.	a.	NOUN
fnp-5883	838	39	,	,	PUNCT
fnp-5883	838	40	streichenberger	streichenberger	NOUN
fnp-5883	838	41	,	,	PUNCT
fnp-5883	838	42	n.	n.	NOUN
fnp-5883	838	43	,	,	PUNCT
fnp-5883	838	44	thomasset	thomasset	NOUN
fnp-5883	838	45	,	,	PUNCT
fnp-5883	838	46	n.	n.	NOUN
fnp-5883	838	47	,	,	PUNCT
fnp-5883	838	48	honnorat	honnorat	PROPN
fnp-5883	838	49	,	,	PUNCT
fnp-5883	838	50	j.	j.	PROPN
fnp-5883	838	51	,	,	PUNCT
fnp-5883	838	52	arzberger	arzberger	NOUN
fnp-5883	838	53	,	,	PUNCT
fnp-5883	838	54	t.	t.	PROPN
fnp-5883	838	55	,	,	PUNCT
fnp-5883	838	56	kretzschmar	kretzschmar	PROPN
fnp-5883	838	57	,	,	PUNCT
fnp-5883	838	58	h.	h.	PROPN
fnp-5883	838	59	,	,	PUNCT
fnp-5883	838	60	2015	2015	NUM
fnp-5883	838	61	.	.	PUNCT
fnp-5883	839	1	the	the	DET
fnp-5883	839	2	workflow	workflow	NOUN
fnp-5883	839	3	from	from	ADP
fnp-5883	839	4	post	post	ADJ
fnp-5883	839	5	-	-	ADJ
fnp-5883	839	6	mortem	mortem	ADJ
fnp-5883	839	7	human	human	ADJ
fnp-5883	839	8	brain	brain	NOUN
fnp-5883	839	9	sampling	sample	VERB
fnp-5883	839	10	to	to	ADP
fnp-5883	839	11	cell	cell	NOUN
fnp-5883	839	12	microdissection	microdissection	NOUN
fnp-5883	839	13	:	:	PUNCT
fnp-5883	839	14	a	a	DET
fnp-5883	839	15	brain	brain	NOUN
fnp-5883	839	16	net	net	PROPN
fnp-5883	839	17	europe	europe	PROPN
fnp-5883	839	18	study	study	PROPN
fnp-5883	839	19	.	.	PUNCT
fnp-5883	840	1	j.	j.	PROPN
fnp-5883	840	2	neural	neural	PROPN
fnp-5883	840	3	transm	transm	PROPN
fnp-5883	840	4	.	.	PUNCT
fnp-5883	841	1	vienna	vienna	PROPN
fnp-5883	841	2	austria	austria	PROPN
fnp-5883	841	3	1996	1996	NUM
fnp-5883	841	4	122	122	NUM
fnp-5883	841	5	,	,	PUNCT
fnp-5883	841	6	975–991	975–991	NUM
fnp-5883	841	7	.	.	PUNCT
fnp-5883	842	1	https://doi.org/10.1007/s00702-015-1378-4	https://doi.org/10.1007/s00702-015-1378-4	NUM
fnp-5883	842	2	möbius	möbius	PROPN
fnp-5883	842	3	,	,	PUNCT
fnp-5883	842	4	w.	w.	PROPN
fnp-5883	842	5	,	,	PUNCT
fnp-5883	842	6	cooper	cooper	PROPN
fnp-5883	842	7	,	,	PUNCT
fnp-5883	842	8	b.	b.	PROPN
fnp-5883	842	9	,	,	PUNCT
fnp-5883	842	10	kaufmann	kaufmann	PROPN
fnp-5883	842	11	,	,	PUNCT
fnp-5883	842	12	w.a	w.a	PROPN
fnp-5883	842	13	.	.	PROPN
fnp-5883	842	14	,	,	PUNCT
fnp-5883	842	15	imig	imig	NOUN
fnp-5883	842	16	,	,	PUNCT
fnp-5883	842	17	c.	c.	NOUN
fnp-5883	842	18	,	,	PUNCT
fnp-5883	842	19	ruhwedel	ruhwedel	NOUN
fnp-5883	842	20	,	,	PUNCT
fnp-5883	842	21	t.	t.	NOUN
fnp-5883	842	22	,	,	PUNCT
fnp-5883	842	23	snaidero	snaidero	NOUN
fnp-5883	842	24	,	,	PUNCT
fnp-5883	842	25	n.	n.	NOUN
fnp-5883	842	26	,	,	PUNCT
fnp-5883	842	27	saab	saab	PROPN
fnp-5883	842	28	,	,	PUNCT
fnp-5883	842	29	a.s	a.s	PROPN
fnp-5883	842	30	.	.	PROPN
fnp-5883	842	31	,	,	PUNCT
fnp-5883	842	32	varoqueaux	varoqueaux	PROPN
fnp-5883	842	33	,	,	PUNCT
fnp-5883	842	34	f.	f.	PROPN
fnp-5883	842	35	,	,	PUNCT
fnp-5883	842	36	2010	2010	NUM
fnp-5883	842	37	.	.	PUNCT
fnp-5883	843	1	electron	electron	NOUN
fnp-5883	843	2	microscopy	microscopy	NOUN
fnp-5883	843	3	of	of	ADP
fnp-5883	843	4	the	the	DET
fnp-5883	843	5	mouse	mouse	NOUN
fnp-5883	843	6	central	central	ADJ
fnp-5883	843	7	nervous	nervous	ADJ
fnp-5883	843	8	system	system	NOUN
fnp-5883	843	9	.	.	PUNCT
fnp-5883	844	1	methods	method	NOUN
fnp-5883	844	2	cell	cell	NOUN
fnp-5883	844	3	biol	biol	PROPN
fnp-5883	844	4	.	.	PUNCT
fnp-5883	845	1	96	96	NUM
fnp-5883	845	2	,	,	PUNCT
fnp-5883	845	3	475–512	475–512	NUM
fnp-5883	845	4	.	.	PUNCT
fnp-5883	845	5	https://doi.org/10.1016/s0091-679x(10)96020-2	https://doi.org/10.1016/s0091-679x(10)96020-2	PROPN
fnp-5883	845	6	moor	moor	PROPN
fnp-5883	845	7	,	,	PUNCT
fnp-5883	845	8	h.	h.	PROPN
fnp-5883	845	9	,	,	PUNCT
fnp-5883	845	10	bellin	bellin	PROPN
fnp-5883	845	11	,	,	PUNCT
fnp-5883	845	12	g.	g.	PROPN
fnp-5883	845	13	,	,	PUNCT
fnp-5883	845	14	sandri	sandri	PROPN
fnp-5883	845	15	,	,	PUNCT
fnp-5883	845	16	c.	c.	PROPN
fnp-5883	845	17	,	,	PUNCT
fnp-5883	845	18	akert	akert	PROPN
fnp-5883	845	19	,	,	PUNCT
fnp-5883	845	20	k.	k.	PROPN
fnp-5883	845	21	,	,	PUNCT
fnp-5883	845	22	1980	1980	NUM
fnp-5883	845	23	.	.	PUNCT
fnp-5883	846	1	the	the	DET
fnp-5883	846	2	influence	influence	NOUN
fnp-5883	846	3	of	of	ADP
fnp-5883	846	4	high	high	ADJ
fnp-5883	846	5	pressure	pressure	NOUN
fnp-5883	846	6	freezing	freezing	NOUN
fnp-5883	846	7	on	on	ADP
fnp-5883	846	8	mammalian	mammalian	ADJ
fnp-5883	846	9	nerve	nerve	NOUN
fnp-5883	846	10	tissue	tissue	NOUN
fnp-5883	846	11	.	.	PUNCT
fnp-5883	847	1	cell	cell	NOUN
fnp-5883	847	2	tissue	tissue	NOUN
fnp-5883	847	3	res	re	NOUN
fnp-5883	847	4	.	.	PUNCT
fnp-5883	848	1	209	209	NUM
fnp-5883	848	2	,	,	PUNCT
fnp-5883	848	3	201–216	201–216	NUM
fnp-5883	848	4	.	.	PUNCT
fnp-5883	849	1	https://doi.org/10.1007/bf00237626	https://doi.org/10.1007/bf00237626	PROPN
fnp-5883	849	2	murray	murray	PROPN
fnp-5883	849	3	,	,	PUNCT
fnp-5883	849	4	k.a	k.a	PROPN
fnp-5883	849	5	.	.	PROPN
fnp-5883	849	6	,	,	PUNCT
fnp-5883	849	7	gibson	gibson	PROPN
fnp-5883	849	8	,	,	PUNCT
fnp-5883	849	9	m.i	m.i	PROPN
fnp-5883	849	10	.	.	PROPN
fnp-5883	849	11	,	,	PUNCT
fnp-5883	849	12	2022	2022	NUM
fnp-5883	849	13	.	.	PUNCT
fnp-5883	850	1	chemical	chemical	NOUN
fnp-5883	850	2	approaches	approach	NOUN
fnp-5883	850	3	to	to	ADP
fnp-5883	850	4	cryopreservation	cryopreservation	NOUN
fnp-5883	850	5	.	.	PUNCT
fnp-5883	851	1	nat	nat	PROPN
fnp-5883	851	2	.	.	PUNCT
fnp-5883	851	3	rev	rev	PROPN
fnp-5883	851	4	.	.	PROPN
fnp-5883	851	5	chem	chem	PROPN
fnp-5883	851	6	.	.	PUNCT
fnp-5883	852	1	6	6	NUM
fnp-5883	852	2	,	,	PUNCT
fnp-5883	852	3	579–593	579–593	NUM
fnp-5883	852	4	.	.	PUNCT
fnp-5883	853	1	https://doi.org/10.1038/s41570-022-00407-4	https://doi.org/10.1038/s41570-022-00407-4	PROPN
fnp-5883	853	2	nochlin	nochlin	PROPN
fnp-5883	853	3	,	,	PUNCT
fnp-5883	853	4	d.	d.	PROPN
fnp-5883	853	5	,	,	PUNCT
fnp-5883	853	6	mackenzie	mackenzie	PROPN
fnp-5883	853	7	,	,	PUNCT
fnp-5883	853	8	a.p	a.p	PROPN
fnp-5883	853	9	.	.	PROPN
fnp-5883	853	10	,	,	PUNCT
fnp-5883	853	11	bryant	bryant	PROPN
fnp-5883	853	12	,	,	PUNCT
fnp-5883	853	13	e.m	e.m	PROPN
fnp-5883	853	14	.	.	PROPN
fnp-5883	853	15	,	,	PUNCT
fnp-5883	853	16	norwood	norwood	PROPN
fnp-5883	853	17	,	,	PUNCT
fnp-5883	853	18	t.h	t.h	PROPN
fnp-5883	853	19	.	.	PROPN
fnp-5883	853	20	,	,	PUNCT
fnp-5883	853	21	sumi	sumi	PROPN
fnp-5883	853	22	,	,	PUNCT
fnp-5883	853	23	s.m	s.m	PROPN
fnp-5883	853	24	.	.	PROPN
fnp-5883	853	25	,	,	PUNCT
fnp-5883	853	26	1993	1993	NUM
fnp-5883	853	27	.	.	PUNCT
fnp-5883	854	1	a	a	DET
fnp-5883	854	2	simple	simple	ADJ
fnp-5883	854	3	method	method	NOUN
fnp-5883	854	4	of	of	ADP
fnp-5883	854	5	rapid	rapid	ADJ
fnp-5883	854	6	freezing	freezing	NOUN
fnp-5883	854	7	adequately	adequately	ADV
fnp-5883	854	8	preserves	preserve	VERB
fnp-5883	854	9	brain	brain	NOUN
fnp-5883	854	10	tissue	tissue	NOUN
fnp-5883	854	11	for	for	ADP
fnp-5883	854	12	immunocytochemistry	immunocytochemistry	NOUN
fnp-5883	854	13	,	,	PUNCT
fnp-5883	854	14	light	light	NOUN
fnp-5883	854	15	and	and	CCONJ
fnp-5883	854	16	electron	electron	VERB
fnp-5883	854	17	microscopic	microscopic	ADJ
fnp-5883	854	18	examination	examination	NOUN
fnp-5883	854	19	.	.	PUNCT
fnp-5883	855	1	acta	acta	PROPN
fnp-5883	855	2	neuropathol	neuropathol	PROPN
fnp-5883	855	3	.	.	PUNCT
fnp-5883	856	1	(	(	PUNCT
fnp-5883	856	2	berl	berl	PROPN
fnp-5883	856	3	.	.	PUNCT
fnp-5883	856	4	)	)	PUNCT
fnp-5883	857	1	86	86	NUM
fnp-5883	857	2	,	,	PUNCT
fnp-5883	858	1	645–650	645–650	NUM
fnp-5883	858	2	.	.	PUNCT
fnp-5883	859	1	https://doi.org/10.1007/bf00294305	https://doi.org/10.1007/bf00294305	NOUN
fnp-5883	859	2	notter	notter	NOUN
fnp-5883	859	3	,	,	PUNCT
fnp-5883	859	4	t.	t.	PROPN
fnp-5883	859	5	,	,	PUNCT
fnp-5883	859	6	panzanelli	panzanelli	PROPN
fnp-5883	859	7	,	,	PUNCT
fnp-5883	859	8	p.	p.	NOUN
fnp-5883	859	9	,	,	PUNCT
fnp-5883	859	10	pfister	pfister	NOUN
fnp-5883	859	11	,	,	PUNCT
fnp-5883	859	12	s.	s.	PROPN
fnp-5883	859	13	,	,	PUNCT
fnp-5883	859	14	mircsof	mircsof	PROPN
fnp-5883	859	15	,	,	PUNCT
fnp-5883	859	16	d.	d.	PROPN
fnp-5883	859	17	,	,	PUNCT
fnp-5883	859	18	fritschy	fritschy	NOUN
fnp-5883	859	19	,	,	PUNCT
fnp-5883	859	20	j.-m	j.-m	NOUN
fnp-5883	859	21	.	.	PROPN
fnp-5883	859	22	,	,	PUNCT
fnp-5883	859	23	2014	2014	NUM
fnp-5883	859	24	.	.	PUNCT
fnp-5883	860	1	a	a	DET
fnp-5883	860	2	protocol	protocol	NOUN
fnp-5883	860	3	for	for	ADP
fnp-5883	860	4	concurrent	concurrent	ADJ
fnp-5883	860	5	high	high	ADJ
fnp-5883	860	6	-	-	PUNCT
fnp-5883	860	7	quality	quality	NOUN
fnp-5883	860	8	immunohistochemical	immunohistochemical	ADJ
fnp-5883	860	9	and	and	CCONJ
fnp-5883	860	10	biochemical	biochemical	ADJ
fnp-5883	860	11	analyses	analysis	NOUN
fnp-5883	860	12	in	in	ADP
fnp-5883	860	13	adult	adult	NOUN
fnp-5883	860	14	mouse	mouse	NOUN
fnp-5883	860	15	central	central	ADJ
fnp-5883	860	16	nervous	nervous	ADJ
fnp-5883	860	17	system	system	NOUN
fnp-5883	860	18	.	.	PUNCT
fnp-5883	861	1	eur	eur	PROPN
fnp-5883	861	2	.	.	PUNCT
fnp-5883	862	1	j.	j.	PROPN
fnp-5883	862	2	neurosci	neurosci	PROPN
fnp-5883	862	3	.	.	PUNCT
fnp-5883	863	1	39	39	NUM
fnp-5883	863	2	,	,	PUNCT
fnp-5883	863	3	165–175	165–175	NUM
fnp-5883	863	4	.	.	PUNCT
fnp-5883	864	1	https://doi.org/10.1111/ejn.12447	https://doi.org/10.1111/ejn.12447	PROPN
fnp-5883	864	2	okotrub	okotrub	PROPN
fnp-5883	864	3	,	,	PUNCT
fnp-5883	864	4	k.a	k.a	PROPN
fnp-5883	864	5	.	.	PROPN
fnp-5883	864	6	,	,	PUNCT
fnp-5883	864	7	surovtsev	surovtsev	PROPN
fnp-5883	864	8	,	,	PUNCT
fnp-5883	864	9	n.v	n.v	PROPN
fnp-5883	864	10	.	.	PROPN
fnp-5883	864	11	,	,	PUNCT
fnp-5883	864	12	2013	2013	NUM
fnp-5883	864	13	.	.	PUNCT
fnp-5883	865	1	raman	raman	NOUN
fnp-5883	865	2	scattering	scatter	VERB
fnp-5883	865	3	evidence	evidence	NOUN
fnp-5883	865	4	of	of	ADP
fnp-5883	865	5	hydrohalite	hydrohalite	ADJ
fnp-5883	865	6	formation	formation	NOUN
fnp-5883	865	7	on	on	ADP
fnp-5883	865	8	frozen	frozen	ADJ
fnp-5883	865	9	yeast	yeast	NOUN
fnp-5883	865	10	cells	cell	NOUN
fnp-5883	865	11	.	.	PUNCT
fnp-5883	866	1	cryobiology	cryobiology	NOUN
fnp-5883	866	2	66	66	NUM
fnp-5883	866	3	,	,	PUNCT
fnp-5883	866	4	47–51	47–51	PROPN
fnp-5883	866	5	.	.	PUNCT
fnp-5883	867	1	https://doi.org/10.1016/j.cryobiol.2012.11.002	https://doi.org/10.1016/j.cryobiol.2012.11.002	PROPN
fnp-5883	867	2	otubo	otubo	PROPN
fnp-5883	867	3	,	,	PUNCT
fnp-5883	867	4	a.	a.	PROPN
fnp-5883	867	5	,	,	PUNCT
fnp-5883	867	6	maejima	maejima	PROPN
fnp-5883	867	7	,	,	PUNCT
fnp-5883	867	8	s.	s.	PROPN
fnp-5883	867	9	,	,	PUNCT
fnp-5883	867	10	oti	oti	PROPN
fnp-5883	867	11	,	,	PUNCT
fnp-5883	867	12	t.	t.	PROPN
fnp-5883	867	13	,	,	PUNCT
fnp-5883	867	14	satoh	satoh	NOUN
fnp-5883	867	15	,	,	PUNCT
fnp-5883	867	16	k.	k.	PROPN
fnp-5883	867	17	,	,	PUNCT
fnp-5883	867	18	ueda	ueda	PROPN
fnp-5883	867	19	,	,	PUNCT
fnp-5883	867	20	y.	y.	PROPN
fnp-5883	867	21	,	,	PUNCT
fnp-5883	867	22	morris	morris	PROPN
fnp-5883	867	23	,	,	PUNCT
fnp-5883	867	24	j.f	j.f	PROPN
fnp-5883	867	25	.	.	PROPN
fnp-5883	867	26	,	,	PUNCT
fnp-5883	867	27	sakamoto	sakamoto	PROPN
fnp-5883	867	28	,	,	PUNCT
fnp-5883	867	29	t.	t.	PROPN
fnp-5883	867	30	,	,	PUNCT
fnp-5883	867	31	sakamoto	sakamoto	PROPN
fnp-5883	867	32	,	,	PUNCT
fnp-5883	867	33	h.	h.	PROPN
fnp-5883	867	34	,	,	PUNCT
fnp-5883	867	35	2021	2021	NUM
fnp-5883	867	36	.	.	PUNCT
fnp-5883	868	1	immunoelectron	immunoelectron	ADJ
fnp-5883	868	2	microscopic	microscopic	ADJ
fnp-5883	868	3	characterization	characterization	NOUN
fnp-5883	868	4	of	of	ADP
fnp-5883	868	5	vasopressin	vasopressin	NOUN
fnp-5883	868	6	-	-	PUNCT
fnp-5883	868	7	producing	produce	VERB
fnp-5883	868	8	neurons	neuron	NOUN
fnp-5883	868	9	in	in	ADP
fnp-5883	868	10	the	the	DET
fnp-5883	868	11	hypothalamo	hypothalamo	NOUN
fnp-5883	868	12	-	-	PUNCT
fnp-5883	868	13	pituitary	pituitary	ADJ
fnp-5883	868	14	axis	axis	NOUN
fnp-5883	868	15	of	of	ADP
fnp-5883	868	16	non	non	ADJ
fnp-5883	868	17	-	-	ADJ
fnp-5883	868	18	human	human	ADJ
fnp-5883	868	19	primates	primate	NOUN
fnp-5883	868	20	by	by	ADP
fnp-5883	868	21	use	use	NOUN
fnp-5883	868	22	of	of	ADP
fnp-5883	868	23	formaldehyde	formaldehyde	NOUN
fnp-5883	868	24	-	-	PUNCT
fnp-5883	868	25	fixed	fix	VERB
fnp-5883	868	26	tissues	tissue	NOUN
fnp-5883	868	27	stored	store	VERB
fnp-5883	868	28	at	at	ADP
fnp-5883	868	29	-25	-25	PROPN
fnp-5883	868	30	 	 	SPACE
fnp-5883	868	31	°	°	ADP
fnp-5883	868	32	c	c	NOUN
fnp-5883	868	33	for	for	ADP
fnp-5883	868	34	several	several	ADJ
fnp-5883	868	35	years	year	NOUN
fnp-5883	868	36	.	.	PUNCT
fnp-5883	869	1	int	int	NOUN
fnp-5883	869	2	.	.	PUNCT
fnp-5883	870	1	j.	j.	PROPN
fnp-5883	870	2	mol	mol	PROPN
fnp-5883	870	3	.	.	PUNCT
fnp-5883	871	1	sci	sci	PROPN
fnp-5883	871	2	.	.	PROPN
fnp-5883	872	1	22	22	NUM
fnp-5883	872	2	,	,	PUNCT
fnp-5883	872	3	9180	9180	NUM
fnp-5883	872	4	.	.	PUNCT
fnp-5883	873	1	https://doi.org/10.3390/ijms22179180	https://doi.org/10.3390/ijms22179180	PROPN
fnp-5883	873	2	ozyoruk	ozyoruk	PROPN
fnp-5883	873	3	,	,	PUNCT
fnp-5883	873	4	k.b	k.b	PROPN
fnp-5883	873	5	.	.	PROPN
fnp-5883	873	6	,	,	PUNCT
fnp-5883	873	7	can	can	AUX
fnp-5883	873	8	,	,	PUNCT
fnp-5883	873	9	s.	s.	PROPN
fnp-5883	873	10	,	,	PUNCT
fnp-5883	873	11	darbaz	darbaz	PROPN
fnp-5883	873	12	,	,	PUNCT
fnp-5883	873	13	b.	b.	PROPN
fnp-5883	873	14	,	,	PUNCT
fnp-5883	873	15	başak	başak	NOUN
fnp-5883	873	16	,	,	PUNCT
fnp-5883	873	17	k.	k.	PROPN
fnp-5883	873	18	,	,	PUNCT
fnp-5883	873	19	demir	demir	PROPN
fnp-5883	873	20	,	,	PUNCT
fnp-5883	873	21	d.	d.	PROPN
fnp-5883	873	22	,	,	PUNCT
fnp-5883	873	23	gokceler	gokceler	PROPN
fnp-5883	873	24	,	,	PUNCT
fnp-5883	873	25	g.i	g.i	PROPN
fnp-5883	873	26	.	.	PROPN
fnp-5883	873	27	,	,	PUNCT
fnp-5883	873	28	serin	serin	NOUN
fnp-5883	873	29	,	,	PUNCT
fnp-5883	873	30	g.	g.	PROPN
fnp-5883	873	31	,	,	PUNCT
fnp-5883	873	32	hacisalihoglu	hacisalihoglu	PROPN
fnp-5883	873	33	,	,	PUNCT
fnp-5883	873	34	u.p	u.p	PROPN
fnp-5883	873	35	.	.	PROPN
fnp-5883	873	36	,	,	PUNCT
fnp-5883	873	37	kurtuluş	kurtuluş	PROPN
fnp-5883	873	38	,	,	PUNCT
fnp-5883	873	39	e.	e.	PROPN
fnp-5883	873	40	,	,	PUNCT
fnp-5883	873	41	lu	lu	PROPN
fnp-5883	873	42	,	,	PUNCT
fnp-5883	873	43	m.y	m.y	PROPN
fnp-5883	873	44	.	.	PROPN
fnp-5883	873	45	,	,	PUNCT
fnp-5883	873	46	chen	chen	PROPN
fnp-5883	873	47	,	,	PUNCT
fnp-5883	873	48	t.y	t.y	PROPN
fnp-5883	873	49	.	.	PROPN
fnp-5883	873	50	,	,	PUNCT
fnp-5883	873	51	williamson	williamson	PROPN
fnp-5883	873	52	,	,	PUNCT
fnp-5883	873	53	d.f.k	d.f.k	PROPN
fnp-5883	873	54	.	.	PROPN
fnp-5883	873	55	,	,	PUNCT
fnp-5883	873	56	yılmaz	yılmaz	PROPN
fnp-5883	873	57	,	,	PUNCT
fnp-5883	873	58	f.	f.	PROPN
fnp-5883	873	59	,	,	PUNCT
fnp-5883	873	60	mahmood	mahmood	PROPN
fnp-5883	873	61	,	,	PUNCT
fnp-5883	873	62	f.	f.	PROPN
fnp-5883	873	63	,	,	PUNCT
fnp-5883	873	64	turan	turan	PROPN
fnp-5883	873	65	,	,	PUNCT
fnp-5883	873	66	m.	m.	NOUN
fnp-5883	873	67	,	,	PUNCT
fnp-5883	873	68	2022	2022	NUM
fnp-5883	873	69	.	.	PUNCT
fnp-5883	874	1	a	a	DET
fnp-5883	874	2	deep	deep	ADV
fnp-5883	874	3	-	-	PUNCT
fnp-5883	874	4	learning	learn	VERB
fnp-5883	874	5	model	model	NOUN
fnp-5883	874	6	for	for	ADP
fnp-5883	874	7	transforming	transform	VERB
fnp-5883	874	8	the	the	DET
fnp-5883	874	9	style	style	NOUN
fnp-5883	874	10	of	of	ADP
fnp-5883	874	11	tissue	tissue	NOUN
fnp-5883	874	12	images	image	NOUN
fnp-5883	874	13	from	from	ADP
fnp-5883	874	14	cryosectioned	cryosectione	VERB
fnp-5883	874	15	to	to	ADP
fnp-5883	874	16	formalin	formalin	NOUN
fnp-5883	874	17	-	-	PUNCT
fnp-5883	874	18	fixed	fix	VERB
fnp-5883	874	19	and	and	CCONJ
fnp-5883	874	20	paraffin	paraffin	NOUN
fnp-5883	874	21	-	-	PUNCT
fnp-5883	874	22	embedded	embed	VERB
fnp-5883	874	23	.	.	PUNCT
fnp-5883	875	1	nat	nat	PROPN
fnp-5883	875	2	.	.	PROPN
fnp-5883	875	3	biomed	biome	VERB
fnp-5883	875	4	.	.	PUNCT
fnp-5883	876	1	eng	eng	PROPN
fnp-5883	876	2	.	.	PROPN
fnp-5883	877	1	6	6	NUM
fnp-5883	877	2	,	,	PUNCT
fnp-5883	877	3	1407–1419	1407–1419	NUM
fnp-5883	877	4	.	.	PUNCT
fnp-5883	878	1	https://doi.org/10.1038/s41551-022-00952-9	https://doi.org/10.1038/s41551-022-00952-9	NOUN
fnp-5883	878	2	paljärvi	paljärvi	PROPN
fnp-5883	878	3	,	,	PUNCT
fnp-5883	878	4	l.	l.	PROPN
fnp-5883	878	5	,	,	PUNCT
fnp-5883	878	6	garcia	garcia	PROPN
fnp-5883	878	7	,	,	PUNCT
fnp-5883	878	8	j.h	j.h	PROPN
fnp-5883	878	9	.	.	PROPN
fnp-5883	878	10	,	,	PUNCT
fnp-5883	878	11	kalimo	kalimo	PROPN
fnp-5883	878	12	,	,	PUNCT
fnp-5883	878	13	h.	h.	PROPN
fnp-5883	878	14	,	,	PUNCT
fnp-5883	878	15	1979	1979	NUM
fnp-5883	878	16	.	.	PUNCT
fnp-5883	879	1	the	the	DET
fnp-5883	879	2	efficiency	efficiency	NOUN
fnp-5883	879	3	of	of	ADP
fnp-5883	879	4	aldehyde	aldehyde	NOUN
fnp-5883	879	5	fixation	fixation	NOUN
fnp-5883	879	6	for	for	ADP
fnp-5883	879	7	electron	electron	NOUN
fnp-5883	879	8	microscopy	microscopy	NOUN
fnp-5883	879	9	:	:	PUNCT
fnp-5883	879	10	stabilization	stabilization	NOUN
fnp-5883	879	11	of	of	ADP
fnp-5883	879	12	rat	rat	NOUN
fnp-5883	879	13	brain	brain	NOUN
fnp-5883	879	14	tissue	tissue	NOUN
fnp-5883	879	15	to	to	PART
fnp-5883	879	16	withstand	withstand	VERB
fnp-5883	879	17	osmotic	osmotic	ADJ
fnp-5883	879	18	stress	stress	NOUN
fnp-5883	879	19	.	.	PUNCT
fnp-5883	880	1	histochem	histochem	PROPN
fnp-5883	880	2	.	.	PUNCT
fnp-5883	881	1	j.	j.	PROPN
fnp-5883	881	2	11	11	NUM
fnp-5883	881	3	,	,	PUNCT
fnp-5883	881	4	267–276	267–276	NUM
fnp-5883	881	5	.	.	PUNCT
fnp-5883	882	1	https://doi.org/10.1007/bf01005026	https://doi.org/10.1007/bf01005026	PROPN
fnp-5883	882	2	paynter	paynter	PROPN
fnp-5883	882	3	,	,	PUNCT
fnp-5883	882	4	s.j	s.j	PROPN
fnp-5883	882	5	.	.	PROPN
fnp-5883	882	6	,	,	PUNCT
fnp-5883	882	7	2008	2008	NUM
fnp-5883	882	8	.	.	PUNCT
fnp-5883	883	1	principles	principle	NOUN
fnp-5883	883	2	and	and	CCONJ
fnp-5883	883	3	practical	practical	ADJ
fnp-5883	883	4	issues	issue	NOUN
fnp-5883	883	5	for	for	ADP
fnp-5883	883	6	cryopreservation	cryopreservation	NOUN
fnp-5883	883	7	of	of	ADP
fnp-5883	883	8	nerve	nerve	NOUN
fnp-5883	883	9	cells	cell	NOUN
fnp-5883	883	10	.	.	PUNCT
fnp-5883	884	1	brain	brain	NOUN
fnp-5883	884	2	res	re	NOUN
fnp-5883	884	3	.	.	PUNCT
fnp-5883	885	1	bull	bull	NOUN
fnp-5883	885	2	.	.	PUNCT
fnp-5883	886	1	75	75	NUM
fnp-5883	886	2	,	,	PUNCT
fnp-5883	886	3	1–14	1–14	PROPN
fnp-5883	886	4	.	.	PROPN
fnp-5883	886	5	https://doi.org/10.1016/j.brainresbull.2007.08.004	https://doi.org/10.1016/j.brainresbull.2007.08.004	PROPN
fnp-5883	886	6	pegg	pegg	PROPN
fnp-5883	886	7	,	,	PUNCT
fnp-5883	886	8	d.e	d.e	PROPN
fnp-5883	886	9	.	.	PROPN
fnp-5883	886	10	,	,	PUNCT
fnp-5883	886	11	2007	2007	NUM
fnp-5883	886	12	.	.	PUNCT
fnp-5883	887	1	principles	principle	NOUN
fnp-5883	887	2	of	of	ADP
fnp-5883	887	3	cryopreservation	cryopreservation	NOUN
fnp-5883	887	4	.	.	PUNCT
fnp-5883	888	1	methods	method	NOUN
fnp-5883	888	2	mol	mol	PROPN
fnp-5883	888	3	.	.	PUNCT
fnp-5883	889	1	biol	biol	PROPN
fnp-5883	889	2	.	.	PUNCT
fnp-5883	890	1	clifton	clifton	PROPN
fnp-5883	890	2	nj	nj	PROPN
fnp-5883	890	3	368	368	NUM
fnp-5883	890	4	,	,	PUNCT
fnp-5883	890	5	39–57	39–57	NUM
fnp-5883	890	6	.	.	PUNCT
fnp-5883	891	1	https://doi.org/10.1007/978-1-59745-362-2_3	https://doi.org/10.1007/978-1-59745-362-2_3	NOUN
fnp-5883	891	2	pegg	pegg	PROPN
fnp-5883	891	3	,	,	PUNCT
fnp-5883	891	4	d.e	d.e	PROPN
fnp-5883	891	5	.	.	PROPN
fnp-5883	891	6	,	,	PUNCT
fnp-5883	891	7	1976	1976	NUM
fnp-5883	891	8	.	.	PUNCT
fnp-5883	892	1	long	long	ADJ
fnp-5883	892	2	-	-	PUNCT
fnp-5883	892	3	term	term	NOUN
fnp-5883	892	4	preservation	preservation	NOUN
fnp-5883	892	5	of	of	ADP
fnp-5883	892	6	cells	cell	NOUN
fnp-5883	892	7	and	and	CCONJ
fnp-5883	892	8	tissues	tissue	NOUN
fnp-5883	892	9	:	:	PUNCT
fnp-5883	892	10	a	a	DET
fnp-5883	892	11	review	review	NOUN
fnp-5883	892	12	.	.	PUNCT
fnp-5883	893	1	j.	j.	PROPN
fnp-5883	893	2	clin	clin	PROPN
fnp-5883	893	3	.	.	PUNCT
fnp-5883	894	1	pathol	pathol	PROPN
fnp-5883	894	2	.	.	PUNCT
fnp-5883	895	1	29	29	NUM
fnp-5883	895	2	,	,	PUNCT
fnp-5883	895	3	271–285	271–285	NUM
fnp-5883	895	4	.	.	PUNCT
fnp-5883	896	1	pérez	pérez	NOUN
fnp-5883	896	2	-	-	PUNCT
fnp-5883	896	3	bermúdez	bermúdez	PROPN
fnp-5883	896	4	,	,	PUNCT
fnp-5883	896	5	i.	i.	PROPN
fnp-5883	896	6	,	,	PUNCT
fnp-5883	896	7	castillo	castillo	PROPN
fnp-5883	896	8	-	-	PUNCT
fnp-5883	896	9	suero	suero	NOUN
fnp-5883	896	10	,	,	PUNCT
fnp-5883	896	11	a.	a.	NOUN
fnp-5883	896	12	,	,	PUNCT
fnp-5883	896	13	cortés	cortés	NOUN
fnp-5883	896	14	-	-	ADJ
fnp-5883	896	15	inostroza	inostroza	ADJ
fnp-5883	896	16	,	,	PUNCT
fnp-5883	896	17	a.	a.	NOUN
fnp-5883	896	18	,	,	PUNCT
fnp-5883	896	19	jeldrez	jeldrez	PROPN
fnp-5883	896	20	,	,	PUNCT
fnp-5883	896	21	c.	c.	PROPN
fnp-5883	896	22	,	,	PUNCT
fnp-5883	896	23	dantas	danta	NOUN
fnp-5883	896	24	,	,	PUNCT
fnp-5883	896	25	a.	a.	NOUN
fnp-5883	896	26	,	,	PUNCT
fnp-5883	896	27	hernández	hernández	PROPN
fnp-5883	896	28	,	,	PUNCT
fnp-5883	896	29	e.	e.	PROPN
fnp-5883	896	30	,	,	PUNCT
fnp-5883	896	31	orellana	orellana	PROPN
fnp-5883	896	32	-	-	PUNCT
fnp-5883	896	33	palma	palma	NOUN
fnp-5883	896	34	,	,	PUNCT
fnp-5883	896	35	p.	p.	NOUN
fnp-5883	896	36	,	,	PUNCT
fnp-5883	896	37	petzold	petzold	PROPN
fnp-5883	896	38	,	,	PUNCT
fnp-5883	896	39	g.	g.	PROPN
fnp-5883	896	40	,	,	PUNCT
fnp-5883	896	41	2023	2023	NUM
fnp-5883	896	42	.	.	PUNCT
fnp-5883	897	1	observation	observation	NOUN
fnp-5883	897	2	and	and	CCONJ
fnp-5883	897	3	measurement	measurement	NOUN
fnp-5883	897	4	of	of	ADP
fnp-5883	897	5	ice	ice	NOUN
fnp-5883	897	6	morphology	morphology	NOUN
fnp-5883	897	7	in	in	ADP
fnp-5883	897	8	foods	food	NOUN
fnp-5883	897	9	:	:	PUNCT
fnp-5883	897	10	a	a	DET
fnp-5883	897	11	review	review	NOUN
fnp-5883	897	12	.	.	PUNCT
fnp-5883	898	1	foods	food	NOUN
fnp-5883	898	2	basel	basel	PROPN
fnp-5883	898	3	switz	switz	PROPN
fnp-5883	898	4	.	.	PUNCT
fnp-5883	899	1	12	12	NUM
fnp-5883	899	2	,	,	PUNCT
fnp-5883	899	3	3987	3987	NUM
fnp-5883	899	4	.	.	PUNCT
fnp-5883	900	1	https://doi.org/10.3390/foods12213987	https://doi.org/10.3390/foods12213987	PROPN
fnp-5883	900	2	peters	peters	PROPN
fnp-5883	900	3	,	,	PUNCT
fnp-5883	900	4	s.	s.	PROPN
fnp-5883	900	5	,	,	PUNCT
fnp-5883	900	6	2010	2010	NUM
fnp-5883	900	7	.	.	PUNCT
fnp-5883	901	1	a	a	DET
fnp-5883	901	2	practical	practical	ADJ
fnp-5883	901	3	guide	guide	NOUN
fnp-5883	901	4	to	to	ADP
fnp-5883	901	5	frozen	frozen	ADJ
fnp-5883	901	6	section	section	NOUN
fnp-5883	901	7	technique	technique	NOUN
fnp-5883	901	8	,	,	PUNCT
fnp-5883	901	9	a	a	DET
fnp-5883	901	10	practical	practical	ADJ
fnp-5883	901	11	guide	guide	NOUN
fnp-5883	901	12	to	to	ADP
fnp-5883	901	13	frozen	frozen	ADJ
fnp-5883	901	14	section	section	NOUN
fnp-5883	901	15	technique	technique	NOUN
fnp-5883	901	16	.	.	PUNCT
fnp-5883	902	1	https://doi.org/10.1007/978-1-4419-1234-3	https://doi.org/10.1007/978-1-4419-1234-3	ADJ
fnp-5883	902	2	petzold	petzold	NOUN
fnp-5883	902	3	,	,	PUNCT
fnp-5883	902	4	g.	g.	PROPN
fnp-5883	902	5	,	,	PUNCT
fnp-5883	902	6	aguilera	aguilera	PROPN
fnp-5883	902	7	,	,	PUNCT
fnp-5883	902	8	j.m	j.m	PROPN
fnp-5883	902	9	.	.	PROPN
fnp-5883	902	10	,	,	PUNCT
fnp-5883	902	11	2009	2009	NUM
fnp-5883	902	12	.	.	PUNCT
fnp-5883	903	1	ice	ice	NOUN
fnp-5883	903	2	morphology	morphology	NOUN
fnp-5883	903	3	:	:	PUNCT
fnp-5883	903	4	fundamentals	fundamental	NOUN
fnp-5883	903	5	and	and	CCONJ
fnp-5883	903	6	technological	technological	ADJ
fnp-5883	903	7	applications	application	NOUN
fnp-5883	903	8	in	in	ADP
fnp-5883	903	9	foods	food	NOUN
fnp-5883	903	10	.	.	PUNCT
fnp-5883	904	1	food	food	NOUN
fnp-5883	904	2	biophys	biophy	NOUN
fnp-5883	904	3	.	.	PUNCT
fnp-5883	905	1	4	4	NUM
fnp-5883	905	2	,	,	PUNCT
fnp-5883	905	3	378–396	378–396	NUM
fnp-5883	905	4	.	.	PUNCT
fnp-5883	906	1	https://doi.org/10.1007/s11483-009-9136-5	https://doi.org/10.1007/s11483-009-9136-5	PROPN
fnp-5883	906	2	pichugin	pichugin	NOUN
fnp-5883	906	3	,	,	PUNCT
fnp-5883	906	4	y.	y.	PROPN
fnp-5883	906	5	,	,	PUNCT
fnp-5883	906	6	fahy	fahy	PROPN
fnp-5883	906	7	,	,	PUNCT
fnp-5883	906	8	g.m	g.m	PROPN
fnp-5883	906	9	.	.	PROPN
fnp-5883	906	10	,	,	PUNCT
fnp-5883	906	11	morin	morin	PROPN
fnp-5883	906	12	,	,	PUNCT
fnp-5883	906	13	r.	r.	PROPN
fnp-5883	906	14	,	,	PUNCT
fnp-5883	906	15	2006	2006	NUM
fnp-5883	906	16	.	.	PUNCT
fnp-5883	907	1	cryopreservation	cryopreservation	NOUN
fnp-5883	907	2	of	of	ADP
fnp-5883	907	3	rat	rat	NOUN
fnp-5883	907	4	hippocampal	hippocampal	ADJ
fnp-5883	907	5	slices	slice	NOUN
fnp-5883	907	6	by	by	ADP
fnp-5883	907	7	vitrification	vitrification	NOUN
fnp-5883	907	8	.	.	PUNCT
fnp-5883	908	1	cryobiology	cryobiology	NOUN
fnp-5883	908	2	52	52	NUM
fnp-5883	908	3	,	,	PUNCT
fnp-5883	908	4	228–240	228–240	NUM
fnp-5883	908	5	.	.	PUNCT
fnp-5883	908	6	https://doi.org/10.1016/j.cryobiol.2005.11.006	https://doi.org/10.1016/j.cryobiol.2005.11.006	PROPN
fnp-5883	908	7	pontén	pontén	NOUN
fnp-5883	908	8	,	,	PUNCT
fnp-5883	908	9	u.	u.	PROPN
fnp-5883	908	10	,	,	PUNCT
fnp-5883	908	11	ratcheson	ratcheson	PROPN
fnp-5883	908	12	,	,	PUNCT
fnp-5883	908	13	r.a	r.a	PROPN
fnp-5883	908	14	.	.	PROPN
fnp-5883	908	15	,	,	PUNCT
fnp-5883	908	16	siesjö	siesjö	PROPN
fnp-5883	908	17	,	,	PUNCT
fnp-5883	908	18	b.k	b.k	PROPN
fnp-5883	908	19	.	.	PROPN
fnp-5883	908	20	,	,	PUNCT
fnp-5883	908	21	1973	1973	NUM
fnp-5883	908	22	.	.	PUNCT
fnp-5883	909	1	metabolic	metabolic	NOUN
fnp-5883	909	2	changes	change	NOUN
fnp-5883	909	3	in	in	ADP
fnp-5883	909	4	the	the	DET
fnp-5883	909	5	brains	brain	NOUN
fnp-5883	909	6	of	of	ADP
fnp-5883	909	7	mice	mouse	NOUN
fnp-5883	909	8	frozen	freeze	VERB
fnp-5883	909	9	in	in	ADP
fnp-5883	909	10	liquid	liquid	ADJ
fnp-5883	909	11	nitrogen	nitrogen	NOUN
fnp-5883	909	12	.	.	PUNCT
fnp-5883	910	1	j.	j.	PROPN
fnp-5883	910	2	neurochem	neurochem	PROPN
fnp-5883	910	3	.	.	PUNCT
fnp-5883	911	1	21	21	NUM
fnp-5883	911	2	,	,	PUNCT
fnp-5883	911	3	1211–1216	1211–1216	NUM
fnp-5883	911	4	.	.	PUNCT
fnp-5883	912	1	priemer	priemer	PROPN
fnp-5883	912	2	,	,	PUNCT
fnp-5883	912	3	d.s	d.s	PROPN
fnp-5883	912	4	.	.	PROPN
fnp-5883	912	5	,	,	PUNCT
fnp-5883	912	6	wysozan	wysozan	PROPN
fnp-5883	912	7	,	,	PUNCT
fnp-5883	912	8	t.	t.	PROPN
fnp-5883	912	9	,	,	PUNCT
fnp-5883	912	10	zahedi	zahedi	PROPN
fnp-5883	912	11	,	,	PUNCT
fnp-5883	912	12	f.	f.	PROPN
fnp-5883	912	13	,	,	PUNCT
fnp-5883	912	14	alrabadi	alrabadi	NOUN
fnp-5883	912	15	,	,	PUNCT
fnp-5883	912	16	n.	n.	PROPN
fnp-5883	912	17	,	,	PUNCT
fnp-5883	912	18	mesa	mesa	PROPN
fnp-5883	912	19	,	,	PUNCT
fnp-5883	912	20	h.	h.	PROPN
fnp-5883	912	21	,	,	PUNCT
fnp-5883	912	22	vortmeyer	vortmeyer	PROPN
fnp-5883	912	23	,	,	PUNCT
fnp-5883	912	24	a.o	a.o	PROPN
fnp-5883	912	25	.	.	PROPN
fnp-5883	912	26	,	,	PUNCT
fnp-5883	912	27	2023	2023	NUM
fnp-5883	912	28	.	.	PUNCT
fnp-5883	913	1	qualitative	qualitative	ADJ
fnp-5883	913	2	comparison	comparison	NOUN
fnp-5883	913	3	of	of	ADP
fnp-5883	913	4	cryostatversus	cryostatversus	PROPN
fnp-5883	913	5	snap	snap	NOUN
fnp-5883	913	6	-	-	PUNCT
fnp-5883	913	7	frozen	frozen	ADJ
fnp-5883	913	8	neurosurgical	neurosurgical	ADJ
fnp-5883	913	9	intraoperative	intraoperative	ADJ
fnp-5883	913	10	consultations	consultation	NOUN
fnp-5883	913	11	.	.	PUNCT
fnp-5883	914	1	int	int	NOUN
fnp-5883	914	2	.	.	PUNCT
fnp-5883	915	1	j.	j.	PROPN
fnp-5883	915	2	surg	surg	PROPN
fnp-5883	915	3	.	.	PUNCT
fnp-5883	916	1	pathol	pathol	PROPN
fnp-5883	916	2	.	.	PUNCT
fnp-5883	917	1	31	31	NUM
fnp-5883	917	2	,	,	PUNCT
fnp-5883	917	3	949–956	949–956	NUM
fnp-5883	917	4	.	.	PUNCT
fnp-5883	917	5	https://doi.org/10.1177/10668969221117987	https://doi.org/10.1177/10668969221117987	X
fnp-5883	918	1	rabin	rabin	PROPN
fnp-5883	918	2	,	,	PUNCT
fnp-5883	918	3	y.	y.	PROPN
fnp-5883	918	4	,	,	PUNCT
fnp-5883	918	5	steif	steif	NOUN
fnp-5883	918	6	,	,	PUNCT
fnp-5883	918	7	p.s	p.s	PROPN
fnp-5883	918	8	.	.	PROPN
fnp-5883	918	9	,	,	PUNCT
fnp-5883	918	10	hess	hess	PROPN
fnp-5883	918	11	,	,	PUNCT
fnp-5883	918	12	k.c	k.c	PROPN
fnp-5883	918	13	.	.	PROPN
fnp-5883	918	14	,	,	PUNCT
fnp-5883	918	15	jimenez	jimenez	PROPN
fnp-5883	918	16	-	-	PUNCT
fnp-5883	918	17	rios	rios	PROPN
fnp-5883	918	18	,	,	PUNCT
fnp-5883	918	19	j.l	j.l	PROPN
fnp-5883	918	20	.	.	PROPN
fnp-5883	918	21	,	,	PUNCT
fnp-5883	918	22	palastro	palastro	X
fnp-5883	918	23	,	,	PUNCT
fnp-5883	918	24	m.c	m.c	PROPN
fnp-5883	918	25	.	.	PROPN
fnp-5883	918	26	,	,	PUNCT
fnp-5883	918	27	2006	2006	NUM
fnp-5883	918	28	.	.	PUNCT
fnp-5883	919	1	fracture	fracture	ADJ
fnp-5883	919	2	formation	formation	NOUN
fnp-5883	919	3	in	in	ADP
fnp-5883	919	4	vitrified	vitrify	VERB
fnp-5883	919	5	thin	thin	ADJ
fnp-5883	919	6	films	film	NOUN
fnp-5883	919	7	of	of	ADP
fnp-5883	919	8	cryoprotectants	cryoprotectant	NOUN
fnp-5883	919	9	.	.	PUNCT
fnp-5883	920	1	cryobiology	cryobiology	NOUN
fnp-5883	920	2	53	53	NUM
fnp-5883	920	3	,	,	PUNCT
fnp-5883	920	4	75–95	75–95	NUM
fnp-5883	920	5	.	.	PUNCT
fnp-5883	921	1	https://doi.org/10.1016/j.cryobiol.2006.03.013	https://doi.org/10.1016/j.cryobiol.2006.03.013	PROPN
fnp-5883	921	2	ramirez	ramirez	PROPN
fnp-5883	921	3	,	,	PUNCT
fnp-5883	921	4	e.p.c	e.p.c	NOUN
fnp-5883	921	5	.	.	PUNCT
fnp-5883	921	6	,	,	PUNCT
fnp-5883	921	7	keller	keller	PROPN
fnp-5883	921	8	,	,	PUNCT
fnp-5883	921	9	c.e	c.e	PROPN
fnp-5883	921	10	.	.	PROPN
fnp-5883	921	11	,	,	PUNCT
fnp-5883	921	12	vonsattel	vonsattel	PROPN
fnp-5883	921	13	,	,	PUNCT
fnp-5883	921	14	j.p	j.p	PROPN
fnp-5883	921	15	.	.	PROPN
fnp-5883	921	16	,	,	PUNCT
fnp-5883	921	17	2018	2018	NUM
fnp-5883	921	18	.	.	PUNCT
fnp-5883	922	1	the	the	DET
fnp-5883	922	2	new	new	PROPN
fnp-5883	922	3	york	york	PROPN
fnp-5883	922	4	brain	brain	PROPN
fnp-5883	922	5	bank	bank	PROPN
fnp-5883	922	6	of	of	ADP
fnp-5883	922	7	columbia	columbia	PROPN
fnp-5883	922	8	university	university	PROPN
fnp-5883	922	9	:	:	PUNCT
fnp-5883	922	10	practical	practical	ADJ
fnp-5883	922	11	highlights	highlight	NOUN
fnp-5883	922	12	of	of	ADP
fnp-5883	922	13	35	35	NUM
fnp-5883	922	14	years	year	NOUN
fnp-5883	922	15	of	of	ADP
fnp-5883	922	16	experience	experience	NOUN
fnp-5883	922	17	.	.	PUNCT
fnp-5883	923	1	handb	handb	NOUN
fnp-5883	923	2	.	.	PUNCT
fnp-5883	924	1	clin	clin	PROPN
fnp-5883	924	2	.	.	PUNCT
fnp-5883	925	1	neurol	neurol	PROPN
fnp-5883	925	2	.	.	PUNCT
fnp-5883	926	1	150	150	NUM
fnp-5883	926	2	,	,	PUNCT
fnp-5883	926	3	105–118	105–118	NUM
fnp-5883	926	4	.	.	PUNCT
fnp-5883	927	1	https://doi.org/10.1016/b978-0-444-63639-3.00008-6	https://doi.org/10.1016/b978-0-444-63639-3.00008-6	PUNCT
fnp-5883	927	2	reger	reger	PROPN
fnp-5883	927	3	,	,	PUNCT
fnp-5883	927	4	j.f	j.f	PROPN
fnp-5883	927	5	.	.	PROPN
fnp-5883	927	6	,	,	PUNCT
fnp-5883	927	7	escaig	escaig	PROPN
fnp-5883	927	8	,	,	PUNCT
fnp-5883	927	9	f.	f.	PROPN
fnp-5883	927	10	,	,	PUNCT
fnp-5883	927	11	1988	1988	NUM
fnp-5883	927	12	.	.	PUNCT
fnp-5883	928	1	a	a	DET
fnp-5883	928	2	comparative	comparative	ADJ
fnp-5883	928	3	fine	fine	ADJ
fnp-5883	928	4	structure	structure	NOUN
fnp-5883	928	5	study	study	NOUN
fnp-5883	928	6	of	of	ADP
fnp-5883	928	7	rat	rat	NOUN
fnp-5883	928	8	cerebral	cerebral	ADJ
fnp-5883	928	9	cortex	cortex	NOUN
fnp-5883	928	10	following	follow	VERB
fnp-5883	928	11	ultra	ultra	ADJ
fnp-5883	928	12	-	-	ADJ
fnp-5883	928	13	rapid	rapid	ADJ
fnp-5883	928	14	freezing	freezing	NOUN
fnp-5883	928	15	and	and	CCONJ
fnp-5883	928	16	conventional	conventional	ADJ
fnp-5883	928	17	chemical	chemical	NOUN
fnp-5883	928	18	fixation	fixation	NOUN
fnp-5883	928	19	procedures	procedure	NOUN
fnp-5883	928	20	.	.	PUNCT
fnp-5883	929	1	j.	j.	PROPN
fnp-5883	929	2	submicrosc	submicrosc	PROPN
fnp-5883	929	3	.	.	PUNCT
fnp-5883	930	1	cytol	cytol	NOUN
fnp-5883	930	2	.	.	PUNCT
fnp-5883	930	3	pathol	pathol	PROPN
fnp-5883	930	4	.	.	PROPN
fnp-5883	931	1	20	20	NUM
fnp-5883	931	2	,	,	PUNCT
fnp-5883	931	3	691–700	691–700	NUM
fnp-5883	931	4	.	.	PUNCT
fnp-5883	932	1	robards	robard	NOUN
fnp-5883	932	2	,	,	PUNCT
fnp-5883	932	3	a.w	a.w	PROPN
fnp-5883	932	4	.	.	PROPN
fnp-5883	932	5	,	,	PUNCT
fnp-5883	932	6	sleytr	sleytr	PROPN
fnp-5883	932	7	,	,	PUNCT
fnp-5883	932	8	u.b	u.b	PROPN
fnp-5883	932	9	.	.	PROPN
fnp-5883	932	10	,	,	PUNCT
fnp-5883	932	11	1985	1985	NUM
fnp-5883	932	12	.	.	PUNCT
fnp-5883	933	1	low	low	ADJ
fnp-5883	933	2	temperature	temperature	NOUN
fnp-5883	933	3	methods	method	NOUN
fnp-5883	933	4	in	in	ADP
fnp-5883	933	5	biological	biological	ADJ
fnp-5883	933	6	electron	electron	NOUN
fnp-5883	933	7	microscopy	microscopy	NOUN
fnp-5883	933	8	.	.	PUNCT
fnp-5883	934	1	elsevier	elsevier	PROPN
fnp-5883	934	2	science	science	PROPN
fnp-5883	934	3	.	.	PUNCT
fnp-5883	935	1	rosene	rosene	PROPN
fnp-5883	935	2	,	,	PUNCT
fnp-5883	935	3	d.l	d.l	PROPN
fnp-5883	935	4	.	.	PROPN
fnp-5883	935	5	,	,	PUNCT
fnp-5883	935	6	rhodes	rhodes	PROPN
fnp-5883	935	7	,	,	PUNCT
fnp-5883	935	8	k.j	k.j	PROPN
fnp-5883	935	9	.	.	PROPN
fnp-5883	935	10	,	,	PUNCT
fnp-5883	935	11	1990	1990	NUM
fnp-5883	935	12	.	.	PUNCT
fnp-5883	936	1	cryoprotection	cryoprotection	NOUN
fnp-5883	936	2	and	and	CCONJ
fnp-5883	936	3	freezing	freezing	ADJ
fnp-5883	936	4	methods	method	NOUN
fnp-5883	936	5	to	to	PART
fnp-5883	936	6	control	control	VERB
fnp-5883	936	7	ice	ice	NOUN
fnp-5883	936	8	crystal	crystal	NOUN
fnp-5883	936	9	artifact	artifact	NOUN
fnp-5883	936	10	in	in	ADP
fnp-5883	936	11	frozen	frozen	ADJ
fnp-5883	936	12	sections	section	NOUN
fnp-5883	936	13	of	of	ADP
fnp-5883	936	14	fixed	fix	VERB
fnp-5883	936	15	and	and	CCONJ
fnp-5883	936	16	unfixed	unfixed	ADJ
fnp-5883	936	17	brain	brain	NOUN
fnp-5883	936	18	tissue	tissue	NOUN
fnp-5883	936	19	,	,	PUNCT
fnp-5883	936	20	in	in	ADP
fnp-5883	936	21	:	:	PUNCT
fnp-5883	936	22	conn	conn	PROPN
fnp-5883	936	23	,	,	PUNCT
fnp-5883	936	24	p.m.	p.m.	PROPN
fnp-5883	936	25	(	(	PUNCT
fnp-5883	936	26	ed	ed	NOUN
fnp-5883	936	27	.	.	PUNCT
fnp-5883	936	28	)	)	PUNCT
fnp-5883	936	29	,	,	PUNCT
fnp-5883	936	30	methods	method	NOUN
fnp-5883	936	31	in	in	ADP
fnp-5883	936	32	neurosciences	neuroscience	NOUN
fnp-5883	936	33	,	,	PUNCT
fnp-5883	936	34	quantitative	quantitative	ADJ
fnp-5883	936	35	and	and	CCONJ
fnp-5883	936	36	qualitative	qualitative	ADJ
fnp-5883	936	37	microscopy	microscopy	NOUN
fnp-5883	936	38	.	.	PUNCT
fnp-5883	937	1	academic	academic	ADJ
fnp-5883	937	2	press	press	NOUN
fnp-5883	937	3	,	,	PUNCT
fnp-5883	937	4	pp	pp	ADJ
fnp-5883	937	5	.	.	PUNCT
fnp-5883	938	1	360–385	360–385	NUM
fnp-5883	938	2	.	.	PUNCT
fnp-5883	939	1	https://doi.org/10.1016/b978-0-12-185255-9.50026-2	https://doi.org/10.1016/b978-0-12-185255-9.50026-2	X
fnp-5883	939	2	rosene	rosene	NOUN
fnp-5883	939	3	,	,	PUNCT
fnp-5883	939	4	d.l	d.l	PROPN
fnp-5883	939	5	.	.	PROPN
fnp-5883	939	6	,	,	PUNCT
fnp-5883	939	7	roy	roy	PROPN
fnp-5883	939	8	,	,	PUNCT
fnp-5883	939	9	n.j	n.j	PROPN
fnp-5883	939	10	.	.	PROPN
fnp-5883	939	11	,	,	PUNCT
fnp-5883	939	12	davis	davis	PROPN
fnp-5883	939	13	,	,	PUNCT
fnp-5883	939	14	b.j	b.j	PROPN
fnp-5883	939	15	.	.	PROPN
fnp-5883	939	16	,	,	PUNCT
fnp-5883	939	17	1986	1986	NUM
fnp-5883	939	18	.	.	PUNCT
fnp-5883	940	1	a	a	DET
fnp-5883	940	2	cryoprotection	cryoprotection	NOUN
fnp-5883	940	3	method	method	NOUN
fnp-5883	940	4	that	that	PRON
fnp-5883	940	5	facilitates	facilitate	VERB
fnp-5883	940	6	cutting	cut	VERB
fnp-5883	940	7	frozen	frozen	ADJ
fnp-5883	940	8	sections	section	NOUN
fnp-5883	940	9	of	of	ADP
fnp-5883	940	10	whole	whole	ADJ
fnp-5883	940	11	monkey	monkey	NOUN
fnp-5883	940	12	brains	brain	NOUN
fnp-5883	940	13	for	for	ADP
fnp-5883	940	14	histological	histological	ADJ
fnp-5883	940	15	and	and	CCONJ
fnp-5883	940	16	histochemical	histochemical	ADJ
fnp-5883	940	17	processing	processing	NOUN
fnp-5883	940	18	without	without	ADP
fnp-5883	940	19	freezing	freeze	VERB
fnp-5883	940	20	artifact	artifact	NOUN
fnp-5883	940	21	.	.	PUNCT
fnp-5883	941	1	j.	j.	PROPN
fnp-5883	941	2	histochem	histochem	PROPN
fnp-5883	941	3	.	.	PUNCT
fnp-5883	942	1	cytochem	cytochem	PROPN
fnp-5883	942	2	.	.	PUNCT
fnp-5883	943	1	off	off	ADV
fnp-5883	943	2	.	.	PUNCT
fnp-5883	944	1	j.	j.	PROPN
fnp-5883	944	2	histochem	histochem	PROPN
fnp-5883	944	3	.	.	PUNCT
fnp-5883	945	1	soc	soc	PROPN
fnp-5883	945	2	.	.	PUNCT
fnp-5883	946	1	34	34	NUM
fnp-5883	946	2	,	,	PUNCT
fnp-5883	946	3	1301–1315	1301–1315	NUM
fnp-5883	946	4	.	.	PUNCT
fnp-5883	947	1	https://doi.org/10.1177/34.10.3745909	https://doi.org/10.1177/34.10.3745909	PROPN
fnp-5883	947	2	rossner	rossner	PROPN
fnp-5883	947	3	,	,	PUNCT
fnp-5883	947	4	m.j	m.j	PROPN
fnp-5883	947	5	.	.	PROPN
fnp-5883	947	6	,	,	PUNCT
fnp-5883	947	7	hirrlinger	hirrlinger	PROPN
fnp-5883	947	8	,	,	PUNCT
fnp-5883	947	9	j.	j.	PROPN
fnp-5883	947	10	,	,	PUNCT
fnp-5883	947	11	wichert	wichert	PROPN
fnp-5883	947	12	,	,	PUNCT
fnp-5883	947	13	s.p	s.p	PROPN
fnp-5883	947	14	.	.	PROPN
fnp-5883	947	15	,	,	PUNCT
fnp-5883	947	16	boehm	boehm	PROPN
fnp-5883	947	17	,	,	PUNCT
fnp-5883	947	18	c.	c.	NOUN
fnp-5883	947	19	,	,	PUNCT
fnp-5883	947	20	newrzella	newrzella	PROPN
fnp-5883	947	21	,	,	PUNCT
fnp-5883	947	22	d.	d.	PROPN
fnp-5883	947	23	,	,	PUNCT
fnp-5883	947	24	hiemisch	hiemisch	PROPN
fnp-5883	947	25	,	,	PUNCT
fnp-5883	947	26	h.	h.	PROPN
fnp-5883	947	27	,	,	PUNCT
fnp-5883	947	28	eisenhardt	eisenhardt	PROPN
fnp-5883	947	29	,	,	PUNCT
fnp-5883	947	30	g.	g.	PROPN
fnp-5883	947	31	,	,	PUNCT
fnp-5883	947	32	stuenkel	stuenkel	NOUN
fnp-5883	947	33	,	,	PUNCT
fnp-5883	947	34	c.	c.	PROPN
fnp-5883	947	35	,	,	PUNCT
fnp-5883	947	36	von	von	PROPN
fnp-5883	947	37	ahsen	ahsen	PROPN
fnp-5883	947	38	,	,	PUNCT
fnp-5883	947	39	o.	o.	PROPN
fnp-5883	947	40	,	,	PUNCT
fnp-5883	947	41	nave	nave	NOUN
fnp-5883	947	42	,	,	PUNCT
fnp-5883	947	43	k.-a	k.-a	PROPN
fnp-5883	947	44	.	.	PROPN
fnp-5883	947	45	,	,	PUNCT
fnp-5883	947	46	2006	2006	NUM
fnp-5883	947	47	.	.	PUNCT
fnp-5883	948	1	global	global	ADJ
fnp-5883	948	2	transcriptome	transcriptome	ADJ
fnp-5883	948	3	analysis	analysis	NOUN
fnp-5883	948	4	of	of	ADP
fnp-5883	948	5	genetically	genetically	ADV
fnp-5883	948	6	identified	identify	VERB
fnp-5883	948	7	neurons	neuron	NOUN
fnp-5883	948	8	in	in	ADP
fnp-5883	948	9	the	the	DET
fnp-5883	948	10	adult	adult	NOUN
fnp-5883	948	11	cortex	cortex	NOUN
fnp-5883	948	12	.	.	PUNCT
fnp-5883	949	1	j.	j.	PROPN
fnp-5883	949	2	neurosci	neurosci	PROPN
fnp-5883	949	3	.	.	PUNCT
fnp-5883	950	1	off	off	ADV
fnp-5883	950	2	.	.	PUNCT
fnp-5883	951	1	j.	j.	PROPN
fnp-5883	951	2	soc	soc	PROPN
fnp-5883	951	3	.	.	PUNCT
fnp-5883	952	1	neurosci	neurosci	PROPN
fnp-5883	952	2	.	.	PUNCT
fnp-5883	953	1	26	26	NUM
fnp-5883	953	2	,	,	PUNCT
fnp-5883	953	3	9956–9966	9956–9966	NUM
fnp-5883	953	4	.	.	PUNCT
fnp-5883	953	5	https://doi.org/10.1523/jneurosci.0468-06.2006	https://doi.org/10.1523/jneurosci.0468-06.2006	NOUN
fnp-5883	953	6	rubinsky	rubinsky	NOUN
fnp-5883	953	7	,	,	PUNCT
fnp-5883	953	8	b.	b.	PROPN
fnp-5883	953	9	,	,	PUNCT
fnp-5883	953	10	pegg	pegg	PROPN
fnp-5883	953	11	,	,	PUNCT
fnp-5883	953	12	d.e	d.e	PROPN
fnp-5883	953	13	.	.	PROPN
fnp-5883	953	14	,	,	PUNCT
fnp-5883	953	15	1988	1988	NUM
fnp-5883	953	16	.	.	PUNCT
fnp-5883	954	1	a	a	DET
fnp-5883	954	2	mathematical	mathematical	ADJ
fnp-5883	954	3	model	model	NOUN
fnp-5883	954	4	for	for	ADP
fnp-5883	954	5	the	the	DET
fnp-5883	954	6	freezing	freezing	ADJ
fnp-5883	954	7	process	process	NOUN
fnp-5883	954	8	in	in	ADP
fnp-5883	954	9	biological	biological	ADJ
fnp-5883	954	10	tissue	tissue	NOUN
fnp-5883	954	11	.	.	PUNCT
fnp-5883	955	1	proc	proc	NOUN
fnp-5883	955	2	.	.	PUNCT
fnp-5883	956	1	r.	r.	PROPN
fnp-5883	956	2	soc	soc	PROPN
fnp-5883	956	3	.	.	PUNCT
fnp-5883	957	1	lond	lond	PROPN
fnp-5883	957	2	.	.	PUNCT
fnp-5883	958	1	b	b	X
fnp-5883	958	2	biol	biol	PROPN
fnp-5883	958	3	.	.	PUNCT
fnp-5883	959	1	sci	sci	PROPN
fnp-5883	959	2	.	.	PROPN
fnp-5883	959	3	234	234	NUM
fnp-5883	959	4	,	,	PUNCT
fnp-5883	959	5	343–358	343–358	NUM
fnp-5883	959	6	.	.	PUNCT
fnp-5883	960	1	https://doi.org/10.1098/rspb.1988.0053	https://doi.org/10.1098/rspb.1988.0053	PRON
fnp-5883	960	2	ryan	ryan	PROPN
fnp-5883	960	3	,	,	PUNCT
fnp-5883	960	4	k.p	k.p	PROPN
fnp-5883	960	5	.	.	PROPN
fnp-5883	960	6	,	,	PUNCT
fnp-5883	960	7	1991	1991	NUM
fnp-5883	960	8	.	.	PUNCT
fnp-5883	961	1	rapid	rapid	ADJ
fnp-5883	961	2	cryogenic	cryogenic	NOUN
fnp-5883	961	3	fixation	fixation	NOUN
fnp-5883	961	4	of	of	ADP
fnp-5883	961	5	biological	biological	ADJ
fnp-5883	961	6	specimens	specimen	NOUN
fnp-5883	961	7	for	for	ADP
fnp-5883	961	8	electron	electron	NOUN
fnp-5883	961	9	microscopy	microscopy	NOUN
fnp-5883	961	10	.	.	PUNCT
fnp-5883	962	1	schäfer	schäfer	NOUN
fnp-5883	962	2	,	,	PUNCT
fnp-5883	962	3	a.t	a.t	PROPN
fnp-5883	962	4	.	.	PROPN
fnp-5883	962	5	,	,	PUNCT
fnp-5883	962	6	kaufmann	kaufmann	PROPN
fnp-5883	962	7	,	,	PUNCT
fnp-5883	962	8	j.d	j.d	PROPN
fnp-5883	962	9	.	.	PROPN
fnp-5883	962	10	,	,	PUNCT
fnp-5883	962	11	1999	1999	NUM
fnp-5883	962	12	.	.	PUNCT
fnp-5883	963	1	what	what	PRON
fnp-5883	963	2	happens	happen	VERB
fnp-5883	963	3	in	in	ADP
fnp-5883	963	4	freezing	freeze	VERB
fnp-5883	963	5	bodies	body	NOUN
fnp-5883	963	6	?	?	PUNCT
fnp-5883	964	1	experimental	experimental	ADJ
fnp-5883	964	2	study	study	NOUN
fnp-5883	964	3	of	of	ADP
fnp-5883	964	4	histological	histological	ADJ
fnp-5883	964	5	tissue	tissue	NOUN
fnp-5883	964	6	change	change	NOUN
fnp-5883	964	7	caused	cause	VERB
fnp-5883	964	8	by	by	ADP
fnp-5883	964	9	freezing	freeze	VERB
fnp-5883	964	10	injuries	injury	NOUN
fnp-5883	964	11	.	.	PUNCT
fnp-5883	965	1	forensic	forensic	ADJ
fnp-5883	965	2	sci	sci	PROPN
fnp-5883	965	3	.	.	PUNCT
fnp-5883	965	4	int	int	PROPN
fnp-5883	965	5	.	.	PUNCT
fnp-5883	966	1	102	102	NUM
fnp-5883	966	2	,	,	PUNCT
fnp-5883	966	3	149–158	149–158	NUM
fnp-5883	966	4	.	.	PUNCT
fnp-5883	967	1	https://doi.org/10.1016/s0379-0738(99)00043-2	https://doi.org/10.1016/s0379-0738(99)00043-2	PROPN
fnp-5883	967	2	seilhean	seilhean	VERB
fnp-5883	967	3	,	,	PUNCT
fnp-5883	967	4	d.	d.	PROPN
fnp-5883	967	5	,	,	PUNCT
fnp-5883	967	6	gansmüller	gansmüller	PROPN
fnp-5883	967	7	,	,	PUNCT
fnp-5883	967	8	a.	a.	NOUN
fnp-5883	967	9	,	,	PUNCT
fnp-5883	967	10	baron	baron	NOUN
fnp-5883	967	11	-	-	PUNCT
fnp-5883	967	12	van	van	NOUN
fnp-5883	967	13	evercooren	evercooren	NOUN
fnp-5883	967	14	,	,	PUNCT
fnp-5883	967	15	a.	a.	NOUN
fnp-5883	967	16	,	,	PUNCT
fnp-5883	967	17	gumpel	gumpel	NOUN
fnp-5883	967	18	,	,	PUNCT
fnp-5883	967	19	m.	m.	NOUN
fnp-5883	967	20	,	,	PUNCT
fnp-5883	967	21	lachapelle	lachapelle	PROPN
fnp-5883	967	22	,	,	PUNCT
fnp-5883	967	23	f.	f.	PROPN
fnp-5883	967	24	,	,	PUNCT
fnp-5883	967	25	1996	1996	NUM
fnp-5883	967	26	.	.	PUNCT
fnp-5883	968	1	myelination	myelination	NOUN
fnp-5883	968	2	by	by	ADP
fnp-5883	968	3	transplanted	transplanted	ADJ
fnp-5883	968	4	human	human	PROPN
fnp-5883	968	5	and	and	CCONJ
fnp-5883	968	6	mouse	mouse	NOUN
fnp-5883	968	7	central	central	ADJ
fnp-5883	968	8	nervous	nervous	ADJ
fnp-5883	968	9	system	system	NOUN
fnp-5883	968	10	tissue	tissue	NOUN
fnp-5883	968	11	after	after	ADP
fnp-5883	968	12	long	long	ADJ
fnp-5883	968	13	-	-	PUNCT
fnp-5883	968	14	term	term	NOUN
fnp-5883	968	15	cryopreservation	cryopreservation	NOUN
fnp-5883	968	16	.	.	PUNCT
fnp-5883	969	1	acta	acta	PROPN
fnp-5883	969	2	neuropathol	neuropathol	PROPN
fnp-5883	969	3	.	.	PUNCT
fnp-5883	970	1	(	(	PUNCT
fnp-5883	970	2	berl	berl	PROPN
fnp-5883	970	3	.	.	PUNCT
fnp-5883	970	4	)	)	PUNCT
fnp-5883	971	1	91	91	NUM
fnp-5883	971	2	,	,	PUNCT
fnp-5883	971	3	82–88	82–88	NUM
fnp-5883	971	4	.	.	PUNCT
fnp-5883	972	1	https://doi.org/10.1007/s004010050396	https://doi.org/10.1007/s004010050396	NUM
fnp-5883	972	2	senda	senda	PROPN
fnp-5883	972	3	,	,	PUNCT
fnp-5883	972	4	t.	t.	PROPN
fnp-5883	972	5	,	,	PUNCT
fnp-5883	972	6	iizuka	iizuka	PROPN
fnp-5883	972	7	-	-	PUNCT
fnp-5883	972	8	kogo	kogo	PROPN
fnp-5883	972	9	,	,	PUNCT
fnp-5883	972	10	a.	a.	PROPN
fnp-5883	972	11	,	,	PUNCT
fnp-5883	972	12	shimomura	shimomura	NOUN
fnp-5883	972	13	,	,	PUNCT
fnp-5883	972	14	a.	a.	NOUN
fnp-5883	972	15	,	,	PUNCT
fnp-5883	972	16	2005	2005	NUM
fnp-5883	972	17	.	.	PUNCT
fnp-5883	973	1	visualization	visualization	NOUN
fnp-5883	973	2	of	of	ADP
fnp-5883	973	3	the	the	DET
fnp-5883	973	4	nuclear	nuclear	ADJ
fnp-5883	973	5	lamina	lamina	NOUN
fnp-5883	973	6	in	in	ADP
fnp-5883	973	7	mouse	mouse	NOUN
fnp-5883	973	8	anterior	anterior	ADJ
fnp-5883	973	9	pituitary	pituitary	ADJ
fnp-5883	973	10	cells	cell	NOUN
fnp-5883	973	11	and	and	CCONJ
fnp-5883	973	12	immunocytochemical	immunocytochemical	ADJ
fnp-5883	973	13	detection	detection	NOUN
fnp-5883	973	14	of	of	ADP
fnp-5883	973	15	lamin	lamin	NOUN
fnp-5883	973	16	a	a	DET
fnp-5883	973	17	/	/	SYM
fnp-5883	973	18	c	c	NOUN
fnp-5883	973	19	by	by	ADP
fnp-5883	973	20	quick	quick	ADJ
fnp-5883	973	21	-	-	PUNCT
fnp-5883	973	22	freeze	freeze	NOUN
fnp-5883	973	23	freeze	freeze	NOUN
fnp-5883	973	24	-	-	PUNCT
fnp-5883	973	25	substitution	substitution	NOUN
fnp-5883	973	26	electron	electron	NOUN
fnp-5883	973	27	microscopy	microscopy	NOUN
fnp-5883	973	28	.	.	PUNCT
fnp-5883	974	1	j.	j.	PROPN
fnp-5883	974	2	histochem	histochem	PROPN
fnp-5883	974	3	.	.	PUNCT
fnp-5883	975	1	cytochem	cytochem	PROPN
fnp-5883	975	2	.	.	PUNCT
fnp-5883	976	1	off	off	ADV
fnp-5883	976	2	.	.	PUNCT
fnp-5883	977	1	j.	j.	PROPN
fnp-5883	977	2	histochem	histochem	PROPN
fnp-5883	977	3	.	.	PUNCT
fnp-5883	978	1	soc	soc	PROPN
fnp-5883	978	2	.	.	PUNCT
fnp-5883	979	1	53	53	NUM
fnp-5883	979	2	,	,	PUNCT
fnp-5883	979	3	497–507	497–507	NUM
fnp-5883	979	4	.	.	PUNCT
fnp-5883	980	1	https://doi.org/10.1369/jhc.4a6478.2005	https://doi.org/10.1369/jhc.4a6478.2005	PROPN
fnp-5883	980	2	shabihkhani	shabihkhani	PROPN
fnp-5883	980	3	,	,	PUNCT
fnp-5883	980	4	m.	m.	NOUN
fnp-5883	980	5	,	,	PUNCT
fnp-5883	980	6	lucey	lucey	PROPN
fnp-5883	980	7	,	,	PUNCT
fnp-5883	980	8	g.m	g.m	PROPN
fnp-5883	980	9	.	.	PROPN
fnp-5883	980	10	,	,	PUNCT
fnp-5883	980	11	wei	wei	PROPN
fnp-5883	980	12	,	,	PUNCT
fnp-5883	980	13	b.	b.	PROPN
fnp-5883	980	14	,	,	PUNCT
fnp-5883	980	15	mareninov	mareninov	PROPN
fnp-5883	980	16	,	,	PUNCT
fnp-5883	980	17	s.	s.	PROPN
fnp-5883	980	18	,	,	PUNCT
fnp-5883	980	19	lou	lou	PROPN
fnp-5883	980	20	,	,	PUNCT
fnp-5883	980	21	j.j	j.j	PROPN
fnp-5883	980	22	.	.	PROPN
fnp-5883	980	23	,	,	PUNCT
fnp-5883	980	24	vinters	vinter	NOUN
fnp-5883	980	25	,	,	PUNCT
fnp-5883	980	26	h.v	h.v	PROPN
fnp-5883	980	27	.	.	PROPN
fnp-5883	980	28	,	,	PUNCT
fnp-5883	980	29	singer	singer	NOUN
fnp-5883	980	30	,	,	PUNCT
fnp-5883	980	31	e.j	e.j	PROPN
fnp-5883	980	32	.	.	PROPN
fnp-5883	980	33	,	,	PUNCT
fnp-5883	980	34	cloughesy	cloughesy	ADJ
fnp-5883	980	35	,	,	PUNCT
fnp-5883	980	36	t.f	t.f	PROPN
fnp-5883	980	37	.	.	PROPN
fnp-5883	980	38	,	,	PUNCT
fnp-5883	980	39	yong	yong	PROPN
fnp-5883	980	40	,	,	PUNCT
fnp-5883	980	41	w.h	w.h	PROPN
fnp-5883	980	42	.	.	PROPN
fnp-5883	980	43	,	,	PUNCT
fnp-5883	980	44	2014	2014	NUM
fnp-5883	980	45	.	.	PUNCT
fnp-5883	981	1	the	the	DET
fnp-5883	981	2	procurement	procurement	NOUN
fnp-5883	981	3	,	,	PUNCT
fnp-5883	981	4	storage	storage	NOUN
fnp-5883	981	5	,	,	PUNCT
fnp-5883	981	6	and	and	CCONJ
fnp-5883	981	7	quality	quality	NOUN
fnp-5883	981	8	assurance	assurance	NOUN
fnp-5883	981	9	of	of	ADP
fnp-5883	981	10	frozen	frozen	ADJ
fnp-5883	981	11	blood	blood	NOUN
fnp-5883	981	12	and	and	CCONJ
fnp-5883	981	13	tissue	tissue	NOUN
fnp-5883	981	14	biospecimens	biospecimen	NOUN
fnp-5883	981	15	in	in	ADP
fnp-5883	981	16	pathology	pathology	NOUN
fnp-5883	981	17	,	,	PUNCT
fnp-5883	981	18	biorepository	biorepository	NOUN
fnp-5883	981	19	,	,	PUNCT
fnp-5883	981	20	and	and	CCONJ
fnp-5883	981	21	biobank	biobank	ADJ
fnp-5883	981	22	settings	setting	NOUN
fnp-5883	981	23	.	.	PUNCT
fnp-5883	982	1	clin	clin	PROPN
fnp-5883	982	2	.	.	PUNCT
fnp-5883	983	1	biochem	biochem	PROPN
fnp-5883	983	2	.	.	PUNCT
fnp-5883	984	1	47	47	NUM
fnp-5883	984	2	,	,	PUNCT
fnp-5883	984	3	258–266	258–266	NUM
fnp-5883	984	4	.	.	PUNCT
fnp-5883	985	1	https://doi.org/10.1016/j.clinbiochem.2014.01.002	https://doi.org/10.1016/j.clinbiochem.2014.01.002	ADJ
fnp-5883	985	2	shepherd	shepherd	NOUN
fnp-5883	985	3	,	,	PUNCT
fnp-5883	985	4	c.e	c.e	PROPN
fnp-5883	985	5	.	.	PROPN
fnp-5883	985	6	,	,	PUNCT
fnp-5883	985	7	alvendia	alvendia	PROPN
fnp-5883	985	8	,	,	PUNCT
fnp-5883	985	9	h.	h.	PROPN
fnp-5883	985	10	,	,	PUNCT
fnp-5883	985	11	halliday	halliday	PROPN
fnp-5883	985	12	,	,	PUNCT
fnp-5883	985	13	g.m	g.m	PROPN
fnp-5883	985	14	.	.	PROPN
fnp-5883	985	15	,	,	PUNCT
fnp-5883	985	16	2019	2019	NUM
fnp-5883	985	17	.	.	PUNCT
fnp-5883	986	1	brain	brain	NOUN
fnp-5883	986	2	banking	banking	NOUN
fnp-5883	986	3	for	for	ADP
fnp-5883	986	4	research	research	NOUN
fnp-5883	986	5	into	into	ADP
fnp-5883	986	6	neurodegenerative	neurodegenerative	ADJ
fnp-5883	986	7	disorders	disorder	NOUN
fnp-5883	986	8	and	and	CCONJ
fnp-5883	986	9	ageing	ageing	NOUN
fnp-5883	986	10	.	.	PUNCT
fnp-5883	987	1	neurosci	neurosci	PROPN
fnp-5883	987	2	.	.	PUNCT
fnp-5883	988	1	bull	bull	NOUN
fnp-5883	988	2	.	.	PUNCT
fnp-5883	989	1	35	35	NUM
fnp-5883	989	2	,	,	PUNCT
fnp-5883	989	3	283–288	283–288	NUM
fnp-5883	989	4	.	.	PUNCT
fnp-5883	990	1	https://doi.org/10.1007/s12264-018-0326-3	https://doi.org/10.1007/s12264-018-0326-3	PROPN
fnp-5883	990	2	siksou	siksou	PROPN
fnp-5883	990	3	,	,	PUNCT
fnp-5883	990	4	l.	l.	PROPN
fnp-5883	990	5	,	,	PUNCT
fnp-5883	990	6	rostaing	rostaing	NOUN
fnp-5883	990	7	,	,	PUNCT
fnp-5883	990	8	p.	p.	NOUN
fnp-5883	990	9	,	,	PUNCT
fnp-5883	990	10	lechaire	lechaire	NOUN
fnp-5883	990	11	,	,	PUNCT
fnp-5883	990	12	j.-p	j.-p	PROPN
fnp-5883	990	13	.	.	PROPN
fnp-5883	990	14	,	,	PUNCT
fnp-5883	990	15	boudier	boudier	NOUN
fnp-5883	990	16	,	,	PUNCT
fnp-5883	990	17	t.	t.	PROPN
fnp-5883	990	18	,	,	PUNCT
fnp-5883	990	19	ohtsuka	ohtsuka	NOUN
fnp-5883	990	20	,	,	PUNCT
fnp-5883	990	21	t.	t.	PROPN
fnp-5883	990	22	,	,	PUNCT
fnp-5883	990	23	fejtová	fejtová	NOUN
fnp-5883	990	24	,	,	PUNCT
fnp-5883	990	25	a.	a.	PROPN
fnp-5883	990	26	,	,	PUNCT
fnp-5883	990	27	kao	kao	PROPN
fnp-5883	990	28	,	,	PUNCT
fnp-5883	990	29	h.-t	h.-t	NOUN
fnp-5883	990	30	.	.	PUNCT
fnp-5883	990	31	,	,	PUNCT
fnp-5883	990	32	greengard	greengard	NOUN
fnp-5883	990	33	,	,	PUNCT
fnp-5883	990	34	p.	p.	NOUN
fnp-5883	990	35	,	,	PUNCT
fnp-5883	990	36	gundelfinger	gundelfinger	NOUN
fnp-5883	990	37	,	,	PUNCT
fnp-5883	990	38	e.d	e.d	PROPN
fnp-5883	990	39	.	.	PROPN
fnp-5883	990	40	,	,	PUNCT
fnp-5883	990	41	triller	triller	PROPN
fnp-5883	990	42	,	,	PUNCT
fnp-5883	990	43	a.	a.	PROPN
fnp-5883	990	44	,	,	PUNCT
fnp-5883	990	45	marty	marty	PROPN
fnp-5883	990	46	,	,	PUNCT
fnp-5883	990	47	s.	s.	PROPN
fnp-5883	990	48	,	,	PUNCT
fnp-5883	990	49	2007	2007	NUM
fnp-5883	990	50	.	.	PUNCT
fnp-5883	991	1	three	three	NUM
fnp-5883	991	2	-	-	PUNCT
fnp-5883	991	3	dimensional	dimensional	ADJ
fnp-5883	991	4	architecture	architecture	NOUN
fnp-5883	991	5	of	of	ADP
fnp-5883	991	6	presynaptic	presynaptic	ADJ
fnp-5883	991	7	terminal	terminal	PROPN
fnp-5883	991	8	cytomatrix	cytomatrix	NOUN
fnp-5883	991	9	.	.	PUNCT
fnp-5883	992	1	j.	j.	PROPN
fnp-5883	992	2	neurosci	neurosci	PROPN
fnp-5883	992	3	.	.	PUNCT
fnp-5883	993	1	off	off	ADV
fnp-5883	993	2	.	.	PUNCT
fnp-5883	994	1	j.	j.	PROPN
fnp-5883	994	2	soc	soc	PROPN
fnp-5883	994	3	.	.	PUNCT
fnp-5883	995	1	neurosci	neurosci	PROPN
fnp-5883	995	2	.	.	PUNCT
fnp-5883	996	1	27	27	NUM
fnp-5883	996	2	,	,	PUNCT
fnp-5883	996	3	6868–6877	6868–6877	NUM
fnp-5883	996	4	.	.	PUNCT
fnp-5883	997	1	https://doi.org/10.1523/jneurosci.1773-07.2007	https://doi.org/10.1523/jneurosci.1773-07.2007	PROPN
fnp-5883	997	2	sosinsky	sosinsky	PROPN
fnp-5883	997	3	,	,	PUNCT
fnp-5883	997	4	g.e	g.e	PROPN
fnp-5883	997	5	.	.	PROPN
fnp-5883	997	6	,	,	PUNCT
fnp-5883	997	7	crum	crum	PROPN
fnp-5883	997	8	,	,	PUNCT
fnp-5883	997	9	j.	j.	PROPN
fnp-5883	997	10	,	,	PUNCT
fnp-5883	997	11	jones	jones	PROPN
fnp-5883	997	12	,	,	PUNCT
fnp-5883	997	13	y.z	y.z	PROPN
fnp-5883	997	14	.	.	PROPN
fnp-5883	997	15	,	,	PUNCT
fnp-5883	997	16	lanman	lanman	PROPN
fnp-5883	997	17	,	,	PUNCT
fnp-5883	997	18	j.	j.	PROPN
fnp-5883	997	19	,	,	PUNCT
fnp-5883	997	20	smarr	smarr	PROPN
fnp-5883	997	21	,	,	PUNCT
fnp-5883	997	22	b.	b.	PROPN
fnp-5883	997	23	,	,	PUNCT
fnp-5883	997	24	terada	terada	PROPN
fnp-5883	997	25	,	,	PUNCT
fnp-5883	997	26	m.	m.	NOUN
fnp-5883	997	27	,	,	PUNCT
fnp-5883	997	28	martone	martone	NOUN
fnp-5883	997	29	,	,	PUNCT
fnp-5883	997	30	m.e	m.e	PROPN
fnp-5883	997	31	.	.	PROPN
fnp-5883	997	32	,	,	PUNCT
fnp-5883	997	33	deerinck	deerinck	PROPN
fnp-5883	997	34	,	,	PUNCT
fnp-5883	997	35	t.j	t.j	PROPN
fnp-5883	997	36	.	.	PROPN
fnp-5883	997	37	,	,	PUNCT
fnp-5883	997	38	johnson	johnson	PROPN
fnp-5883	997	39	,	,	PUNCT
fnp-5883	997	40	j.e	j.e	PROPN
fnp-5883	997	41	.	.	PROPN
fnp-5883	997	42	,	,	PUNCT
fnp-5883	997	43	ellisman	ellisman	PROPN
fnp-5883	997	44	,	,	PUNCT
fnp-5883	997	45	m.h	m.h	PROPN
fnp-5883	997	46	.	.	PROPN
fnp-5883	997	47	,	,	PUNCT
fnp-5883	997	48	2008	2008	NUM
fnp-5883	997	49	.	.	PUNCT
fnp-5883	998	1	the	the	DET
fnp-5883	998	2	combination	combination	NOUN
fnp-5883	998	3	of	of	ADP
fnp-5883	998	4	chemical	chemical	ADJ
fnp-5883	998	5	fixation	fixation	NOUN
fnp-5883	998	6	procedures	procedure	NOUN
fnp-5883	998	7	with	with	ADP
fnp-5883	998	8	high	high	ADJ
fnp-5883	998	9	pressure	pressure	NOUN
fnp-5883	998	10	freezing	freezing	NOUN
fnp-5883	998	11	and	and	CCONJ
fnp-5883	998	12	freeze	freeze	VERB
fnp-5883	998	13	substitution	substitution	NOUN
fnp-5883	998	14	preserves	preserve	VERB
fnp-5883	998	15	highly	highly	ADV
fnp-5883	998	16	labile	labile	ADJ
fnp-5883	998	17	tissue	tissue	NOUN
fnp-5883	998	18	ultrastructure	ultrastructure	NOUN
fnp-5883	998	19	for	for	ADP
fnp-5883	998	20	electron	electron	NOUN
fnp-5883	998	21	tomography	tomography	NOUN
fnp-5883	998	22	applications	application	NOUN
fnp-5883	998	23	.	.	PUNCT
fnp-5883	999	1	j.	j.	PROPN
fnp-5883	999	2	struct	struct	PROPN
fnp-5883	999	3	.	.	PUNCT
fnp-5883	1000	1	biol	biol	PROPN
fnp-5883	1000	2	.	.	PUNCT
fnp-5883	1001	1	161	161	NUM
fnp-5883	1001	2	,	,	PUNCT
fnp-5883	1001	3	359–371	359–371	NUM
fnp-5883	1001	4	.	.	PUNCT
fnp-5883	1001	5	https://doi.org/10.1016/j.jsb.2007.09.002	https://doi.org/10.1016/j.jsb.2007.09.002	NOUN
fnp-5883	1001	6	studer	studer	NOUN
fnp-5883	1001	7	,	,	PUNCT
fnp-5883	1001	8	d.	d.	PROPN
fnp-5883	1001	9	,	,	PUNCT
fnp-5883	1001	10	zhao	zhao	PROPN
fnp-5883	1001	11	,	,	PUNCT
fnp-5883	1001	12	s.	s.	PROPN
fnp-5883	1001	13	,	,	PUNCT
fnp-5883	1001	14	chai	chai	NOUN
fnp-5883	1001	15	,	,	PUNCT
fnp-5883	1001	16	x.	x.	PROPN
fnp-5883	1001	17	,	,	PUNCT
fnp-5883	1001	18	jonas	jonas	PROPN
fnp-5883	1001	19	,	,	PUNCT
fnp-5883	1001	20	p.	p.	PROPN
fnp-5883	1001	21	,	,	PUNCT
fnp-5883	1001	22	graber	graber	PROPN
fnp-5883	1001	23	,	,	PUNCT
fnp-5883	1001	24	w.	w.	PROPN
fnp-5883	1001	25	,	,	PUNCT
fnp-5883	1001	26	nestel	nestel	PROPN
fnp-5883	1001	27	,	,	PUNCT
fnp-5883	1001	28	s.	s.	PROPN
fnp-5883	1001	29	,	,	PUNCT
fnp-5883	1001	30	frotscher	frotscher	ADJ
fnp-5883	1001	31	,	,	PUNCT
fnp-5883	1001	32	m.	m.	NOUN
fnp-5883	1001	33	,	,	PUNCT
fnp-5883	1001	34	2014	2014	NUM
fnp-5883	1001	35	.	.	PUNCT
fnp-5883	1002	1	capture	capture	NOUN
fnp-5883	1002	2	of	of	ADP
fnp-5883	1002	3	activity	activity	NOUN
fnp-5883	1002	4	-	-	PUNCT
fnp-5883	1002	5	induced	induce	VERB
fnp-5883	1002	6	ultrastructural	ultrastructural	ADJ
fnp-5883	1002	7	changes	change	NOUN
fnp-5883	1002	8	at	at	ADP
fnp-5883	1002	9	synapses	synapsis	NOUN
fnp-5883	1002	10	by	by	ADP
fnp-5883	1002	11	high	high	ADJ
fnp-5883	1002	12	-	-	PUNCT
fnp-5883	1002	13	pressure	pressure	NOUN
fnp-5883	1002	14	freezing	freezing	NOUN
fnp-5883	1002	15	of	of	ADP
fnp-5883	1002	16	brain	brain	NOUN
fnp-5883	1002	17	tissue	tissue	NOUN
fnp-5883	1002	18	.	.	PUNCT
fnp-5883	1003	1	nat	nat	NOUN
fnp-5883	1003	2	.	.	PUNCT
fnp-5883	1004	1	protoc	protoc	VERB
fnp-5883	1004	2	.	.	PUNCT
fnp-5883	1005	1	9	9	NUM
fnp-5883	1005	2	,	,	PUNCT
fnp-5883	1005	3	1480–1495	1480–1495	NUM
fnp-5883	1005	4	.	.	PUNCT
fnp-5883	1006	1	https://doi.org/10.1038/nprot.2014.099	https://doi.org/10.1038/nprot.2014.099	PROPN
fnp-5883	1006	2	suda	suda	PROPN
fnp-5883	1006	3	,	,	PUNCT
fnp-5883	1006	4	i.	i.	PROPN
fnp-5883	1006	5	,	,	PUNCT
fnp-5883	1006	6	kito	kito	PROPN
fnp-5883	1006	7	,	,	PUNCT
fnp-5883	1006	8	k.	k.	PROPN
fnp-5883	1006	9	,	,	PUNCT
fnp-5883	1006	10	adachi	adachi	PROPN
fnp-5883	1006	11	,	,	PUNCT
fnp-5883	1006	12	c.	c.	PROPN
fnp-5883	1006	13	,	,	PUNCT
fnp-5883	1006	14	1966	1966	NUM
fnp-5883	1006	15	.	.	PUNCT
fnp-5883	1007	1	viability	viability	NOUN
fnp-5883	1007	2	of	of	ADP
fnp-5883	1007	3	long	long	ADJ
fnp-5883	1007	4	term	term	NOUN
fnp-5883	1007	5	frozen	frozen	ADJ
fnp-5883	1007	6	cat	cat	NOUN
fnp-5883	1007	7	brain	brain	NOUN
fnp-5883	1007	8	in	in	ADP
fnp-5883	1007	9	vitro	vitro	X
fnp-5883	1007	10	.	.	PUNCT
fnp-5883	1008	1	nature	nature	NOUN
fnp-5883	1008	2	212	212	NUM
fnp-5883	1008	3	,	,	PUNCT
fnp-5883	1008	4	268–270	268–270	NUM
fnp-5883	1008	5	.	.	PUNCT
fnp-5883	1009	1	https://doi.org/10.1038/212268a0	https://doi.org/10.1038/212268a0	PROPN
fnp-5883	1009	2	szabo	szabo	PROPN
fnp-5883	1009	3	,	,	PUNCT
fnp-5883	1009	4	g.v	g.v	PROPN
fnp-5883	1009	5	.	.	PROPN
fnp-5883	1009	6	,	,	PUNCT
fnp-5883	1009	7	burg	burg	PROPN
fnp-5883	1009	8	,	,	PUNCT
fnp-5883	1009	9	t.p	t.p	PROPN
fnp-5883	1009	10	.	.	PROPN
fnp-5883	1009	11	,	,	PUNCT
fnp-5883	1009	12	2024	2024	NUM
fnp-5883	1009	13	.	.	PUNCT
fnp-5883	1009	14	time	time	NOUN
fnp-5883	1009	15	resolved	resolve	VERB
fnp-5883	1009	16	cryo	cryo	NOUN
fnp-5883	1009	17	-	-	PUNCT
fnp-5883	1009	18	correlative	correlative	ADJ
fnp-5883	1009	19	light	light	NOUN
fnp-5883	1009	20	and	and	CCONJ
fnp-5883	1009	21	electron	electron	NOUN
fnp-5883	1009	22	microscopy	microscopy	NOUN
fnp-5883	1009	23	.	.	PUNCT
fnp-5883	1010	1	adv	adv	PROPN
fnp-5883	1010	2	.	.	PUNCT
fnp-5883	1011	1	funct	funct	PROPN
fnp-5883	1011	2	.	.	PUNCT
fnp-5883	1012	1	mater	mater	PROPN
fnp-5883	1012	2	.	.	PUNCT
fnp-5883	1013	1	34	34	NUM
fnp-5883	1013	2	,	,	PUNCT
fnp-5883	1013	3	2313705	2313705	NUM
fnp-5883	1013	4	.	.	PUNCT
fnp-5883	1014	1	https://doi.org/10.1002/adfm.202313705	https://doi.org/10.1002/adfm.202313705	NOUN
fnp-5883	1014	2	taqi	taqi	NOUN
fnp-5883	1014	3	,	,	PUNCT
fnp-5883	1014	4	s.a	s.a	PROPN
fnp-5883	1014	5	.	.	PROPN
fnp-5883	1014	6	,	,	PUNCT
fnp-5883	1014	7	sami	sami	PROPN
fnp-5883	1014	8	,	,	PUNCT
fnp-5883	1014	9	s.a	s.a	PROPN
fnp-5883	1014	10	.	.	PROPN
fnp-5883	1014	11	,	,	PUNCT
fnp-5883	1014	12	sami	sami	PROPN
fnp-5883	1014	13	,	,	PUNCT
fnp-5883	1014	14	l.b	l.b	PROPN
fnp-5883	1014	15	.	.	PROPN
fnp-5883	1014	16	,	,	PUNCT
fnp-5883	1014	17	zaki	zaki	PROPN
fnp-5883	1014	18	,	,	PUNCT
fnp-5883	1014	19	s.a	s.a	PROPN
fnp-5883	1014	20	.	.	PROPN
fnp-5883	1014	21	,	,	PUNCT
fnp-5883	1014	22	2018	2018	NUM
fnp-5883	1014	23	.	.	PUNCT
fnp-5883	1015	1	a	a	DET
fnp-5883	1015	2	review	review	NOUN
fnp-5883	1015	3	of	of	ADP
fnp-5883	1015	4	artifacts	artifact	NOUN
fnp-5883	1015	5	in	in	ADP
fnp-5883	1015	6	histopathology	histopathology	NOUN
fnp-5883	1015	7	.	.	PUNCT
fnp-5883	1016	1	j.	j.	PROPN
fnp-5883	1016	2	oral	oral	PROPN
fnp-5883	1016	3	maxillofac	maxillofac	NOUN
fnp-5883	1016	4	.	.	PUNCT
fnp-5883	1017	1	pathol	pathol	NOUN
fnp-5883	1017	2	.	.	PUNCT
fnp-5883	1018	1	jomfp	jomfp	PROPN
fnp-5883	1018	2	22	22	NUM
fnp-5883	1018	3	,	,	PUNCT
fnp-5883	1018	4	279	279	NUM
fnp-5883	1018	5	.	.	PUNCT
fnp-5883	1019	1	https://doi.org/10.4103/jomfp.jomfp_125_15	https://doi.org/10.4103/jomfp.jomfp_125_15	PUNCT
fnp-5883	1019	2	tian	tian	ADJ
fnp-5883	1019	3	,	,	PUNCT
fnp-5883	1019	4	m.	m.	NOUN
fnp-5883	1019	5	,	,	PUNCT
fnp-5883	1019	6	reger	reger	PROPN
fnp-5883	1019	7	,	,	PUNCT
fnp-5883	1019	8	j.f	j.f	PROPN
fnp-5883	1019	9	.	.	PROPN
fnp-5883	1019	10	,	,	PUNCT
fnp-5883	1019	11	armstrong	armstrong	PROPN
fnp-5883	1019	12	,	,	PUNCT
fnp-5883	1019	13	w.e	w.e	PROPN
fnp-5883	1019	14	.	.	PROPN
fnp-5883	1019	15	,	,	PUNCT
fnp-5883	1019	16	1991	1991	NUM
fnp-5883	1019	17	.	.	PUNCT
fnp-5883	1020	1	electron	electron	NOUN
fnp-5883	1020	2	microscopic	microscopic	NOUN
fnp-5883	1020	3	and	and	CCONJ
fnp-5883	1020	4	immunocytochemical	immunocytochemical	ADJ
fnp-5883	1020	5	study	study	NOUN
fnp-5883	1020	6	of	of	ADP
fnp-5883	1020	7	rapidly	rapidly	ADV
fnp-5883	1020	8	frozen	freeze	VERB
fnp-5883	1020	9	,	,	PUNCT
fnp-5883	1020	10	freeze	freeze	NOUN
fnp-5883	1020	11	-	-	PUNCT
fnp-5883	1020	12	substituted	substitute	VERB
fnp-5883	1020	13	neural	neural	ADJ
fnp-5883	1020	14	lobes	lobe	NOUN
fnp-5883	1020	15	of	of	ADP
fnp-5883	1020	16	rats	rat	NOUN
fnp-5883	1020	17	.	.	PUNCT
fnp-5883	1021	1	j.	j.	PROPN
fnp-5883	1021	2	neurocytol	neurocytol	PROPN
fnp-5883	1021	3	.	.	PUNCT
fnp-5883	1022	1	20	20	NUM
fnp-5883	1022	2	,	,	PUNCT
fnp-5883	1022	3	79–96	79–96	NUM
fnp-5883	1022	4	.	.	PUNCT
fnp-5883	1023	1	https://doi.org/10.1007/bf01279613	https://doi.org/10.1007/bf01279613	PROPN
fnp-5883	1023	2	tofte	tofte	PROPN
fnp-5883	1023	3	,	,	PUNCT
fnp-5883	1023	4	k.	k.	PROPN
fnp-5883	1023	5	,	,	PUNCT
fnp-5883	1023	6	berger	berger	PROPN
fnp-5883	1023	7	,	,	PUNCT
fnp-5883	1023	8	c.	c.	PROPN
fnp-5883	1023	9	,	,	PUNCT
fnp-5883	1023	10	torp	torp	PROPN
fnp-5883	1023	11	,	,	PUNCT
fnp-5883	1023	12	s.h	s.h	PROPN
fnp-5883	1023	13	.	.	PROPN
fnp-5883	1023	14	,	,	PUNCT
fnp-5883	1023	15	solheim	solheim	PROPN
fnp-5883	1023	16	,	,	PUNCT
fnp-5883	1023	17	o.	o.	NOUN
fnp-5883	1023	18	,	,	PUNCT
fnp-5883	1023	19	2014	2014	NUM
fnp-5883	1023	20	.	.	PUNCT
fnp-5883	1024	1	the	the	DET
fnp-5883	1024	2	diagnostic	diagnostic	ADJ
fnp-5883	1024	3	properties	property	NOUN
fnp-5883	1024	4	of	of	ADP
fnp-5883	1024	5	frozen	frozen	ADJ
fnp-5883	1024	6	sections	section	NOUN
fnp-5883	1024	7	in	in	ADP
fnp-5883	1024	8	suspected	suspect	VERB
fnp-5883	1024	9	intracranial	intracranial	ADJ
fnp-5883	1024	10	tumors	tumor	NOUN
fnp-5883	1024	11	:	:	PUNCT
fnp-5883	1024	12	a	a	DET
fnp-5883	1024	13	study	study	NOUN
fnp-5883	1024	14	of	of	ADP
fnp-5883	1024	15	578	578	NUM
fnp-5883	1024	16	consecutive	consecutive	ADJ
fnp-5883	1024	17	cases	case	NOUN
fnp-5883	1024	18	.	.	PUNCT
fnp-5883	1025	1	surg	surg	NOUN
fnp-5883	1025	2	.	.	PUNCT
fnp-5883	1026	1	neurol	neurol	NOUN
fnp-5883	1026	2	.	.	PUNCT
fnp-5883	1027	1	int	int	NOUN
fnp-5883	1027	2	.	.	PUNCT
fnp-5883	1028	1	5	5	NUM
fnp-5883	1028	2	,	,	PUNCT
fnp-5883	1028	3	170	170	NUM
fnp-5883	1028	4	.	.	PUNCT
fnp-5883	1029	1	https://doi.org/10.4103/2152-7806.146153	https://doi.org/10.4103/2152-7806.146153	VERB
fnp-5883	1029	2	tourtellotte	tourtellotte	NOUN
fnp-5883	1029	3	,	,	PUNCT
fnp-5883	1029	4	w.w	w.w	PROPN
fnp-5883	1029	5	.	.	PROPN
fnp-5883	1029	6	,	,	PUNCT
fnp-5883	1029	7	rosario	rosario	PROPN
fnp-5883	1029	8	,	,	PUNCT
fnp-5883	1029	9	i.p	i.p	PROPN
fnp-5883	1029	10	.	.	PROPN
fnp-5883	1029	11	,	,	PUNCT
fnp-5883	1029	12	conrad	conrad	PROPN
fnp-5883	1029	13	,	,	PUNCT
fnp-5883	1029	14	a.	a.	PROPN
fnp-5883	1029	15	,	,	PUNCT
fnp-5883	1029	16	syndulko	syndulko	PROPN
fnp-5883	1029	17	,	,	PUNCT
fnp-5883	1029	18	k.	k.	PROPN
fnp-5883	1029	19	,	,	PUNCT
fnp-5883	1029	20	1993	1993	NUM
fnp-5883	1029	21	.	.	PUNCT
fnp-5883	1030	1	human	human	ADJ
fnp-5883	1030	2	neuro	neuro	PROPN
fnp-5883	1030	3	-	-	PUNCT
fnp-5883	1030	4	specimen	speciman	NOUN
fnp-5883	1030	5	banking	bank	VERB
fnp-5883	1030	6	1961	1961	NUM
fnp-5883	1030	7	-	-	SYM
fnp-5883	1030	8	1992	1992	NUM
fnp-5883	1030	9	.	.	PUNCT
fnp-5883	1031	1	the	the	DET
fnp-5883	1031	2	national	national	ADJ
fnp-5883	1031	3	neurological	neurological	ADJ
fnp-5883	1031	4	research	research	NOUN
fnp-5883	1031	5	specimen	speciman	NOUN
fnp-5883	1031	6	bank	bank	PROPN
fnp-5883	1031	7	(	(	PUNCT
fnp-5883	1031	8	a	a	DET
fnp-5883	1031	9	donor	donor	NOUN
fnp-5883	1031	10	program	program	NOUN
fnp-5883	1031	11	of	of	ADP
fnp-5883	1031	12	preand	preand	NOUN
fnp-5883	1031	13	post	post	ADJ
fnp-5883	1031	14	-	-	ADJ
fnp-5883	1031	15	mortem	mortem	ADJ
fnp-5883	1031	16	tissues	tissue	NOUN
fnp-5883	1031	17	and	and	CCONJ
fnp-5883	1031	18	cerebrospinal	cerebrospinal	ADJ
fnp-5883	1031	19	fluid	fluid	NOUN
fnp-5883	1031	20	/	/	SYM
fnp-5883	1031	21	blood	blood	NOUN
fnp-5883	1031	22	;	;	PUNCT
fnp-5883	1031	23	and	and	CCONJ
fnp-5883	1031	24	a	a	DET
fnp-5883	1031	25	collection	collection	NOUN
fnp-5883	1031	26	of	of	ADP
fnp-5883	1031	27	cryopreserved	cryopreserved	ADJ
fnp-5883	1031	28	human	human	ADJ
fnp-5883	1031	29	neurological	neurological	ADJ
fnp-5883	1031	30	specimens	specimen	NOUN
fnp-5883	1031	31	for	for	ADP
fnp-5883	1031	32	neuroscientists	neuroscientist	NOUN
fnp-5883	1031	33	)	)	PUNCT
fnp-5883	1031	34	.	.	PUNCT
fnp-5883	1032	1	j.	j.	PROPN
fnp-5883	1032	2	neural	neural	PROPN
fnp-5883	1032	3	transm	transm	PROPN
fnp-5883	1032	4	.	.	PUNCT
fnp-5883	1033	1	suppl	suppl	PROPN
fnp-5883	1033	2	.	.	PROPN
fnp-5883	1033	3	39	39	NUM
fnp-5883	1033	4	,	,	PUNCT
fnp-5883	1033	5	5–15	5–15	PROPN
fnp-5883	1033	6	.	.	PUNCT
fnp-5883	1034	1	trump	trump	NOUN
fnp-5883	1034	2	,	,	PUNCT
fnp-5883	1034	3	b.f	b.f	PROPN
fnp-5883	1034	4	.	.	PROPN
fnp-5883	1034	5	,	,	PUNCT
fnp-5883	1034	6	young	young	PROPN
fnp-5883	1034	7	,	,	PUNCT
fnp-5883	1034	8	d.e	d.e	PROPN
fnp-5883	1034	9	.	.	PROPN
fnp-5883	1034	10	,	,	PUNCT
fnp-5883	1034	11	arnold	arnold	PROPN
fnp-5883	1034	12	,	,	PUNCT
fnp-5883	1034	13	e.a	e.a	PROPN
fnp-5883	1034	14	.	.	PROPN
fnp-5883	1034	15	,	,	PUNCT
fnp-5883	1034	16	stowell	stowell	PROPN
fnp-5883	1034	17	,	,	PUNCT
fnp-5883	1034	18	r.e	r.e	PROPN
fnp-5883	1034	19	.	.	PROPN
fnp-5883	1034	20	,	,	PUNCT
fnp-5883	1034	21	1965	1965	NUM
fnp-5883	1034	22	.	.	PUNCT
fnp-5883	1035	1	effects	effect	NOUN
fnp-5883	1035	2	of	of	ADP
fnp-5883	1035	3	freezing	freeze	VERB
fnp-5883	1035	4	and	and	CCONJ
fnp-5883	1035	5	thawing	thaw	VERB
fnp-5883	1035	6	on	on	ADP
fnp-5883	1035	7	the	the	DET
fnp-5883	1035	8	structure	structure	NOUN
fnp-5883	1035	9	,	,	PUNCT
fnp-5883	1035	10	chemical	chemical	NOUN
fnp-5883	1035	11	constitution	constitution	NOUN
fnp-5883	1035	12	,	,	PUNCT
fnp-5883	1035	13	and	and	CCONJ
fnp-5883	1035	14	function	function	NOUN
fnp-5883	1035	15	of	of	ADP
fnp-5883	1035	16	cytoplasmic	cytoplasmic	ADJ
fnp-5883	1035	17	structures	structure	NOUN
fnp-5883	1035	18	.	.	PUNCT
fnp-5883	1036	1	fed	fed	PROPN
fnp-5883	1036	2	.	.	PUNCT
fnp-5883	1037	1	proc	proc	PROPN
fnp-5883	1037	2	.	.	PUNCT
fnp-5883	1038	1	24	24	NUM
fnp-5883	1038	2	,	,	PUNCT
fnp-5883	1038	3	s144	s144	PROPN
fnp-5883	1038	4	-	-	SYM
fnp-5883	1038	5	168	168	NUM
fnp-5883	1038	6	.	.	PUNCT
fnp-5883	1039	1	ulfig	ulfig	PROPN
fnp-5883	1039	2	,	,	PUNCT
fnp-5883	1039	3	n.	n.	NOUN
fnp-5883	1039	4	,	,	PUNCT
fnp-5883	1039	5	nickel	nickel	NOUN
fnp-5883	1039	6	,	,	PUNCT
fnp-5883	1039	7	j.	j.	PROPN
fnp-5883	1039	8	,	,	PUNCT
fnp-5883	1039	9	bohl	bohl	PROPN
fnp-5883	1039	10	,	,	PUNCT
fnp-5883	1039	11	j.	j.	PROPN
fnp-5883	1039	12	,	,	PUNCT
fnp-5883	1039	13	1998	1998	NUM
fnp-5883	1039	14	.	.	PUNCT
fnp-5883	1040	1	monoclonal	monoclonal	NOUN
fnp-5883	1040	2	antibodies	antibodie	VERB
fnp-5883	1040	3	smi	smi	PROPN
fnp-5883	1040	4	311	311	NUM
fnp-5883	1040	5	and	and	CCONJ
fnp-5883	1040	6	smi	smi	PROPN
fnp-5883	1040	7	312	312	NUM
fnp-5883	1040	8	as	as	ADP
fnp-5883	1040	9	tools	tool	NOUN
fnp-5883	1040	10	to	to	PART
fnp-5883	1040	11	investigate	investigate	VERB
fnp-5883	1040	12	the	the	DET
fnp-5883	1040	13	maturation	maturation	NOUN
fnp-5883	1040	14	of	of	ADP
fnp-5883	1040	15	nerve	nerve	NOUN
fnp-5883	1040	16	cells	cell	NOUN
fnp-5883	1040	17	and	and	CCONJ
fnp-5883	1040	18	axonal	axonal	ADJ
fnp-5883	1040	19	patterns	pattern	NOUN
fnp-5883	1040	20	in	in	ADP
fnp-5883	1040	21	human	human	ADJ
fnp-5883	1040	22	fetal	fetal	ADJ
fnp-5883	1040	23	brain	brain	NOUN
fnp-5883	1040	24	.	.	PUNCT
fnp-5883	1041	1	cell	cell	NOUN
fnp-5883	1041	2	tissue	tissue	NOUN
fnp-5883	1041	3	res	re	NOUN
fnp-5883	1041	4	.	.	PUNCT
fnp-5883	1041	5	291	291	NUM
fnp-5883	1041	6	,	,	PUNCT
fnp-5883	1041	7	433–443	433–443	NUM
fnp-5883	1041	8	.	.	PUNCT
fnp-5883	1042	1	https://doi.org/10.1007/s004410051013	https://doi.org/10.1007/s004410051013	NUM
fnp-5883	1042	2	van	van	PROPN
fnp-5883	1042	3	der	der	NOUN
fnp-5883	1042	4	wijngaart	wijngaart	NOUN
fnp-5883	1042	5	,	,	PUNCT
fnp-5883	1042	6	h.	h.	PROPN
fnp-5883	1042	7	,	,	PUNCT
fnp-5883	1042	8	jagga	jagga	PROPN
fnp-5883	1042	9	,	,	PUNCT
fnp-5883	1042	10	s.	s.	PROPN
fnp-5883	1042	11	,	,	PUNCT
fnp-5883	1042	12	dekker	dekker	PROPN
fnp-5883	1042	13	,	,	PUNCT
fnp-5883	1042	14	h.	h.	PROPN
fnp-5883	1042	15	,	,	PUNCT
fnp-5883	1042	16	de	de	PROPN
fnp-5883	1042	17	goeij	goeij	PROPN
fnp-5883	1042	18	,	,	PUNCT
fnp-5883	1042	19	r.	r.	PROPN
fnp-5883	1042	20	,	,	PUNCT
fnp-5883	1042	21	piersma	piersma	PROPN
fnp-5883	1042	22	,	,	PUNCT
fnp-5883	1042	23	s.r	s.r	PROPN
fnp-5883	1042	24	.	.	PROPN
fnp-5883	1042	25	,	,	PUNCT
fnp-5883	1042	26	pham	pham	PROPN
fnp-5883	1042	27	,	,	PUNCT
fnp-5883	1042	28	t.v	t.v	PROPN
fnp-5883	1042	29	.	.	PROPN
fnp-5883	1042	30	,	,	PUNCT
fnp-5883	1042	31	knol	knol	PROPN
fnp-5883	1042	32	,	,	PUNCT
fnp-5883	1042	33	j.c	j.c	PROPN
fnp-5883	1042	34	.	.	PROPN
fnp-5883	1042	35	,	,	PUNCT
fnp-5883	1042	36	zonderhuis	zonderhuis	PROPN
fnp-5883	1042	37	,	,	PUNCT
fnp-5883	1042	38	b.m	b.m	AUX
fnp-5883	1042	39	.	.	PROPN
fnp-5883	1042	40	,	,	PUNCT
fnp-5883	1042	41	holland	holland	PROPN
fnp-5883	1042	42	,	,	PUNCT
fnp-5883	1042	43	h.j	h.j	PROPN
fnp-5883	1042	44	.	.	PROPN
fnp-5883	1042	45	,	,	PUNCT
fnp-5883	1042	46	jiménez	jiménez	PROPN
fnp-5883	1042	47	,	,	PUNCT
fnp-5883	1042	48	c.r	c.r	PROPN
fnp-5883	1042	49	.	.	PROPN
fnp-5883	1042	50	,	,	PUNCT
fnp-5883	1042	51	verheul	verheul	ADJ
fnp-5883	1042	52	,	,	PUNCT
fnp-5883	1042	53	h.m.w	h.m.w	INTJ
fnp-5883	1042	54	.	.	PROPN
fnp-5883	1042	55	,	,	PUNCT
fnp-5883	1042	56	vanapalli	vanapalli	PROPN
fnp-5883	1042	57	,	,	PUNCT
fnp-5883	1042	58	s.	s.	PROPN
fnp-5883	1042	59	,	,	PUNCT
fnp-5883	1042	60	labots	labot	NOUN
fnp-5883	1042	61	,	,	PUNCT
fnp-5883	1042	62	m.	m.	NOUN
fnp-5883	1042	63	,	,	PUNCT
fnp-5883	1042	64	2023	2023	NUM
fnp-5883	1042	65	.	.	PUNCT
fnp-5883	1043	1	advancing	advance	VERB
fnp-5883	1043	2	wide	wide	ADJ
fnp-5883	1043	3	implementation	implementation	NOUN
fnp-5883	1043	4	of	of	ADP
fnp-5883	1043	5	precision	precision	NOUN
fnp-5883	1043	6	oncology	oncology	NOUN
fnp-5883	1043	7	:	:	PUNCT
fnp-5883	1043	8	a	a	DET
fnp-5883	1043	9	liquid	liquid	ADJ
fnp-5883	1043	10	nitrogen	nitrogen	NOUN
fnp-5883	1043	11	-	-	PUNCT
fnp-5883	1043	12	free	free	ADJ
fnp-5883	1043	13	snap	snap	NOUN
fnp-5883	1043	14	freezer	freezer	NOUN
fnp-5883	1043	15	preserves	preserve	VERB
fnp-5883	1043	16	molecular	molecular	ADJ
fnp-5883	1043	17	profiles	profile	NOUN
fnp-5883	1043	18	of	of	ADP
fnp-5883	1043	19	biological	biological	ADJ
fnp-5883	1043	20	samples	sample	NOUN
fnp-5883	1043	21	.	.	PUNCT
fnp-5883	1044	1	cancer	cancer	NOUN
fnp-5883	1044	2	med	med	PROPN
fnp-5883	1044	3	.	.	PROPN
fnp-5883	1044	4	12	12	NUM
fnp-5883	1044	5	,	,	PUNCT
fnp-5883	1044	6	10979–10989	10979–10989	NUM
fnp-5883	1044	7	.	.	PUNCT
fnp-5883	1045	1	https://doi.org/10.1002/cam4.5781	https://doi.org/10.1002/cam4.5781	X
fnp-5883	1046	1	vanhecke	vanhecke	PROPN
fnp-5883	1046	2	,	,	PUNCT
fnp-5883	1046	3	d.	d.	PROPN
fnp-5883	1046	4	,	,	PUNCT
fnp-5883	1046	5	graber	graber	PROPN
fnp-5883	1046	6	,	,	PUNCT
fnp-5883	1046	7	w.	w.	PROPN
fnp-5883	1046	8	,	,	PUNCT
fnp-5883	1046	9	studer	studer	NOUN
fnp-5883	1046	10	,	,	PUNCT
fnp-5883	1046	11	d.	d.	PROPN
fnp-5883	1046	12	,	,	PUNCT
fnp-5883	1046	13	2010	2010	NUM
fnp-5883	1046	14	.	.	PUNCT
fnp-5883	1047	1	rapidly	rapidly	ADV
fnp-5883	1047	2	excised	excise	VERB
fnp-5883	1047	3	and	and	CCONJ
fnp-5883	1047	4	cryofixed	cryofixe	VERB
fnp-5883	1047	5	rat	rat	NOUN
fnp-5883	1047	6	tissue	tissue	NOUN
fnp-5883	1047	7	.	.	PUNCT
fnp-5883	1048	1	methods	method	NOUN
fnp-5883	1048	2	cell	cell	NOUN
fnp-5883	1048	3	biol	biol	PROPN
fnp-5883	1048	4	.	.	PUNCT
fnp-5883	1049	1	96	96	NUM
fnp-5883	1049	2	,	,	PUNCT
fnp-5883	1049	3	513–527	513–527	NUM
fnp-5883	1049	4	.	.	PUNCT
fnp-5883	1050	1	https://doi.org/10.1016/s0091-679x(10)96021-4	https://doi.org/10.1016/s0091-679x(10)96021-4	PROPN
fnp-5883	1050	2	vonsattel	vonsattel	PROPN
fnp-5883	1050	3	,	,	PUNCT
fnp-5883	1050	4	j.p	j.p	PROPN
fnp-5883	1050	5	.	.	PROPN
fnp-5883	1050	6	,	,	PUNCT
fnp-5883	1050	7	aizawa	aizawa	PROPN
fnp-5883	1050	8	,	,	PUNCT
fnp-5883	1050	9	h.	h.	PROPN
fnp-5883	1050	10	,	,	PUNCT
fnp-5883	1050	11	ge	ge	PROPN
fnp-5883	1050	12	,	,	PUNCT
fnp-5883	1050	13	p.	p.	PROPN
fnp-5883	1050	14	,	,	PUNCT
fnp-5883	1050	15	difiglia	difiglia	PROPN
fnp-5883	1050	16	,	,	PUNCT
fnp-5883	1050	17	m.	m.	NOUN
fnp-5883	1050	18	,	,	PUNCT
fnp-5883	1050	19	mckee	mckee	PROPN
fnp-5883	1050	20	,	,	PUNCT
fnp-5883	1050	21	a.c	a.c	PROPN
fnp-5883	1050	22	.	.	PROPN
fnp-5883	1050	23	,	,	PUNCT
fnp-5883	1050	24	macdonald	macdonald	PROPN
fnp-5883	1050	25	,	,	PUNCT
fnp-5883	1050	26	m.	m.	NOUN
fnp-5883	1050	27	,	,	PUNCT
fnp-5883	1050	28	gusella	gusella	PROPN
fnp-5883	1050	29	,	,	PUNCT
fnp-5883	1050	30	j.f	j.f	PROPN
fnp-5883	1050	31	.	.	PROPN
fnp-5883	1050	32	,	,	PUNCT
fnp-5883	1050	33	landwehrmeyer	landwehrmeyer	PROPN
fnp-5883	1050	34	,	,	PUNCT
fnp-5883	1050	35	g.b	g.b	PROPN
fnp-5883	1050	36	.	.	PROPN
fnp-5883	1050	37	,	,	PUNCT
fnp-5883	1050	38	bird	bird	PROPN
fnp-5883	1050	39	,	,	PUNCT
fnp-5883	1050	40	e.d	e.d	PROPN
fnp-5883	1050	41	.	.	PROPN
fnp-5883	1050	42	,	,	PUNCT
fnp-5883	1050	43	richardson	richardson	PROPN
fnp-5883	1050	44	,	,	PUNCT
fnp-5883	1050	45	e.p	e.p	PROPN
fnp-5883	1050	46	.	.	PROPN
fnp-5883	1050	47	,	,	PUNCT
fnp-5883	1050	48	1995	1995	NUM
fnp-5883	1050	49	.	.	PUNCT
fnp-5883	1051	1	an	an	DET
fnp-5883	1051	2	improved	improved	ADJ
fnp-5883	1051	3	approach	approach	NOUN
fnp-5883	1051	4	to	to	PART
fnp-5883	1051	5	prepare	prepare	VERB
fnp-5883	1051	6	human	human	ADJ
fnp-5883	1051	7	brains	brain	NOUN
fnp-5883	1051	8	for	for	ADP
fnp-5883	1051	9	research	research	NOUN
fnp-5883	1051	10	.	.	PUNCT
fnp-5883	1052	1	j.	j.	PROPN
fnp-5883	1052	2	neuropathol	neuropathol	PROPN
fnp-5883	1052	3	.	.	PUNCT
fnp-5883	1053	1	exp	exp	PROPN
fnp-5883	1053	2	.	.	PUNCT
fnp-5883	1054	1	neurol	neurol	PROPN
fnp-5883	1054	2	.	.	PUNCT
fnp-5883	1055	1	54	54	NUM
fnp-5883	1055	2	,	,	PUNCT
fnp-5883	1055	3	42–56	42–56	NUM
fnp-5883	1055	4	.	.	PUNCT
fnp-5883	1056	1	https://doi.org/10.1097/00005072-199501000-00006	https://doi.org/10.1097/00005072-199501000-00006	PROPN
fnp-5883	1056	2	vonsattel	vonsattel	PROPN
fnp-5883	1056	3	,	,	PUNCT
fnp-5883	1056	4	j.p.g	j.p.g	PROPN
fnp-5883	1056	5	.	.	PROPN
fnp-5883	1056	6	,	,	PUNCT
fnp-5883	1056	7	amaya	amaya	PROPN
fnp-5883	1056	8	,	,	PUNCT
fnp-5883	1056	9	m.	m.	NOUN
fnp-5883	1056	10	del	del	PROPN
fnp-5883	1056	11	p.	p.	PROPN
fnp-5883	1056	12	,	,	PUNCT
fnp-5883	1056	13	cortes	corte	NOUN
fnp-5883	1056	14	,	,	PUNCT
fnp-5883	1056	15	e.p	e.p	PROPN
fnp-5883	1056	16	.	.	PROPN
fnp-5883	1056	17	,	,	PUNCT
fnp-5883	1056	18	mancevska	mancevska	PROPN
fnp-5883	1056	19	,	,	PUNCT
fnp-5883	1056	20	k.	k.	PROPN
fnp-5883	1056	21	,	,	PUNCT
fnp-5883	1056	22	keller	keller	PROPN
fnp-5883	1056	23	,	,	PUNCT
fnp-5883	1056	24	c.e	c.e	PROPN
fnp-5883	1056	25	.	.	PROPN
fnp-5883	1056	26	,	,	PUNCT
fnp-5883	1056	27	2008	2008	NUM
fnp-5883	1056	28	.	.	PUNCT
fnp-5883	1057	1	21st	21st	ADJ
fnp-5883	1057	2	century	century	NOUN
fnp-5883	1057	3	brain	brain	NOUN
fnp-5883	1057	4	banking	banking	NOUN
fnp-5883	1057	5	practical	practical	ADJ
fnp-5883	1057	6	prerequisites	prerequisite	NOUN
fnp-5883	1057	7	and	and	CCONJ
fnp-5883	1057	8	lessons	lesson	NOUN
fnp-5883	1057	9	from	from	ADP
fnp-5883	1057	10	the	the	DET
fnp-5883	1057	11	past	past	NOUN
fnp-5883	1057	12	:	:	PUNCT
fnp-5883	1057	13	the	the	DET
fnp-5883	1057	14	experience	experience	NOUN
fnp-5883	1057	15	of	of	ADP
fnp-5883	1057	16	new	new	PROPN
fnp-5883	1057	17	york	york	PROPN
fnp-5883	1057	18	brain	brain	PROPN
fnp-5883	1057	19	bank	bank	PROPN
fnp-5883	1057	20	–	–	PUNCT
fnp-5883	1057	21	taub	taub	PROPN
fnp-5883	1057	22	institute	institute	PROPN
fnp-5883	1057	23	columbia	columbia	PROPN
fnp-5883	1057	24	university	university	PROPN
fnp-5883	1057	25	.	.	PUNCT
fnp-5883	1058	1	cell	cell	NOUN
fnp-5883	1058	2	tissue	tissue	NOUN
fnp-5883	1058	3	bank	bank	NOUN
fnp-5883	1058	4	.	.	PUNCT
fnp-5883	1059	1	9	9	NUM
fnp-5883	1059	2	,	,	PUNCT
fnp-5883	1059	3	247–258	247–258	NUM
fnp-5883	1059	4	.	.	PUNCT
fnp-5883	1060	1	https://doi.org/10.1007/s10561-008-9079-y	https://doi.org/10.1007/s10561-008-9079-y	PROPN
fnp-5883	1060	2	watson	watson	PROPN
fnp-5883	1060	3	,	,	PUNCT
fnp-5883	1060	4	r.e.j	r.e.j	PROPN
fnp-5883	1060	5	.	.	PROPN
fnp-5883	1060	6	,	,	PUNCT
fnp-5883	1060	7	wiegand	wiegand	PROPN
fnp-5883	1060	8	,	,	PUNCT
fnp-5883	1060	9	s.j	s.j	PROPN
fnp-5883	1060	10	.	.	PROPN
fnp-5883	1060	11	,	,	PUNCT
fnp-5883	1060	12	clough	clough	PROPN
fnp-5883	1060	13	,	,	PUNCT
fnp-5883	1060	14	r.w	r.w	PROPN
fnp-5883	1060	15	.	.	PROPN
fnp-5883	1060	16	,	,	PUNCT
fnp-5883	1060	17	hoffman	hoffman	PROPN
fnp-5883	1060	18	,	,	PUNCT
fnp-5883	1060	19	g.e	g.e	PROPN
fnp-5883	1060	20	.	.	PROPN
fnp-5883	1060	21	,	,	PUNCT
fnp-5883	1060	22	1986	1986	NUM
fnp-5883	1060	23	.	.	PUNCT
fnp-5883	1061	1	use	use	NOUN
fnp-5883	1061	2	of	of	ADP
fnp-5883	1061	3	cryoprotectant	cryoprotectant	NOUN
fnp-5883	1061	4	to	to	PART
fnp-5883	1061	5	maintain	maintain	VERB
fnp-5883	1061	6	long	long	ADJ
fnp-5883	1061	7	-	-	PUNCT
fnp-5883	1061	8	term	term	NOUN
fnp-5883	1061	9	peptide	peptide	NOUN
fnp-5883	1061	10	immunoreactivity	immunoreactivity	NOUN
fnp-5883	1061	11	and	and	CCONJ
fnp-5883	1061	12	tissue	tissue	NOUN
fnp-5883	1061	13	morphology	morphology	NOUN
fnp-5883	1061	14	.	.	PUNCT
fnp-5883	1062	1	peptides	peptide	NOUN
fnp-5883	1062	2	7	7	NUM
fnp-5883	1062	3	,	,	PUNCT
fnp-5883	1062	4	155–159	155–159	NUM
fnp-5883	1062	5	.	.	PUNCT
fnp-5883	1063	1	https://doi.org/10.1016/0196-9781(86)90076-8	https://doi.org/10.1016/0196-9781(86)90076-8	PROPN
fnp-5883	1063	2	weng	weng	PROPN
fnp-5883	1063	3	,	,	PUNCT
fnp-5883	1063	4	l.	l.	PROPN
fnp-5883	1063	5	,	,	PUNCT
fnp-5883	1063	6	stott	stott	PROPN
fnp-5883	1063	7	,	,	PUNCT
fnp-5883	1063	8	s.l	s.l	PROPN
fnp-5883	1063	9	.	.	PROPN
fnp-5883	1063	10	,	,	PUNCT
fnp-5883	1063	11	toner	toner	NOUN
fnp-5883	1063	12	,	,	PUNCT
fnp-5883	1063	13	m.	m.	NOUN
fnp-5883	1063	14	,	,	PUNCT
fnp-5883	1063	15	2019	2019	NUM
fnp-5883	1063	16	.	.	PUNCT
fnp-5883	1064	1	exploring	explore	VERB
fnp-5883	1064	2	dynamics	dynamic	NOUN
fnp-5883	1064	3	and	and	CCONJ
fnp-5883	1064	4	structure	structure	NOUN
fnp-5883	1064	5	of	of	ADP
fnp-5883	1064	6	biomolecules	biomolecule	NOUN
fnp-5883	1064	7	,	,	PUNCT
fnp-5883	1064	8	cryoprotectants	cryoprotectant	NOUN
fnp-5883	1064	9	,	,	PUNCT
fnp-5883	1064	10	and	and	CCONJ
fnp-5883	1064	11	water	water	NOUN
fnp-5883	1064	12	using	use	VERB
fnp-5883	1064	13	molecular	molecular	ADJ
fnp-5883	1064	14	dynamics	dynamic	NOUN
fnp-5883	1064	15	simulations	simulation	NOUN
fnp-5883	1064	16	:	:	PUNCT
fnp-5883	1064	17	implications	implication	NOUN
fnp-5883	1064	18	for	for	ADP
fnp-5883	1064	19	biostabilization	biostabilization	NOUN
fnp-5883	1064	20	and	and	CCONJ
fnp-5883	1064	21	biopreservation	biopreservation	NOUN
fnp-5883	1064	22	.	.	PUNCT
fnp-5883	1065	1	annu	annu	PROPN
fnp-5883	1065	2	.	.	PUNCT
fnp-5883	1066	1	rev	rev	PROPN
fnp-5883	1066	2	.	.	PROPN
fnp-5883	1066	3	biomed	biome	VERB
fnp-5883	1066	4	.	.	PUNCT
fnp-5883	1067	1	eng	eng	PROPN
fnp-5883	1067	2	.	.	PROPN
fnp-5883	1067	3	21	21	NUM
fnp-5883	1067	4	,	,	PUNCT
fnp-5883	1067	5	1–31	1–31	PROPN
fnp-5883	1067	6	.	.	PUNCT
fnp-5883	1068	1	https://doi.org/10.1146/annurev-bioeng-060418-052130	https://doi.org/10.1146/annurev-bioeng-060418-052130	PROPN
fnp-5883	1068	2	wieser	wieser	PROPN
fnp-5883	1068	3	,	,	PUNCT
fnp-5883	1068	4	m.	m.	NOUN
fnp-5883	1068	5	,	,	PUNCT
fnp-5883	1068	6	burger	burger	NOUN
fnp-5883	1068	7	,	,	PUNCT
fnp-5883	1068	8	s.	s.	PROPN
fnp-5883	1068	9	,	,	PUNCT
fnp-5883	1068	10	ertl	ertl	PROPN
fnp-5883	1068	11	,	,	PUNCT
fnp-5883	1068	12	r.	r.	PROPN
fnp-5883	1068	13	,	,	PUNCT
fnp-5883	1068	14	kummer	kummer	PROPN
fnp-5883	1068	15	,	,	PUNCT
fnp-5883	1068	16	s.	s.	PROPN
fnp-5883	1068	17	,	,	PUNCT
fnp-5883	1068	18	stargardt	stargardt	PROPN
fnp-5883	1068	19	,	,	PUNCT
fnp-5883	1068	20	m.	m.	NOUN
fnp-5883	1068	21	,	,	PUNCT
fnp-5883	1068	22	walter	walter	PROPN
fnp-5883	1068	23	,	,	PUNCT
fnp-5883	1068	24	i.	i.	PROPN
fnp-5883	1068	25	,	,	PUNCT
fnp-5883	1068	26	2022	2022	NUM
fnp-5883	1068	27	.	.	PUNCT
fnp-5883	1069	1	example	example	NOUN
fnp-5883	1069	2	for	for	ADP
fnp-5883	1069	3	process	process	NOUN
fnp-5883	1069	4	validation	validation	NOUN
fnp-5883	1069	5	in	in	ADP
fnp-5883	1069	6	biobanking	biobanking	NOUN
fnp-5883	1069	7	:	:	PUNCT
fnp-5883	1069	8	fit	fit	ADJ
fnp-5883	1069	9	for	for	ADP
fnp-5883	1069	10	purpose	purpose	NOUN
fnp-5883	1069	11	testing	testing	NOUN
fnp-5883	1069	12	of	of	ADP
fnp-5883	1069	13	a	a	DET
fnp-5883	1069	14	cryopreservation	cryopreservation	NOUN
fnp-5883	1069	15	method	method	NOUN
fnp-5883	1069	16	without	without	ADP
fnp-5883	1069	17	isopentane	isopentane	NOUN
fnp-5883	1069	18	.	.	PUNCT
fnp-5883	1070	1	front	front	PROPN
fnp-5883	1070	2	.	.	PUNCT
fnp-5883	1071	1	mol	mol	PROPN
fnp-5883	1071	2	.	.	PUNCT
fnp-5883	1072	1	biosci	biosci	PROPN
fnp-5883	1072	2	.	.	PUNCT
fnp-5883	1073	1	9	9	NUM
fnp-5883	1073	2	,	,	PUNCT
fnp-5883	1073	3	876670	876670	NUM
fnp-5883	1073	4	.	.	PUNCT
fnp-5883	1074	1	https://doi.org/10.3389/fmolb.2022.876670	https://doi.org/10.3389/fmolb.2022.876670	PROPN
fnp-5883	1074	2	wong	wong	PROPN
fnp-5883	1074	3	,	,	PUNCT
fnp-5883	1074	4	g.	g.	PROPN
fnp-5883	1074	5	,	,	PUNCT
fnp-5883	1074	6	greenhalgh	greenhalgh	PROPN
fnp-5883	1074	7	,	,	PUNCT
fnp-5883	1074	8	t.	t.	PROPN
fnp-5883	1074	9	,	,	PUNCT
fnp-5883	1074	10	westhorp	westhorp	PROPN
fnp-5883	1074	11	,	,	PUNCT
fnp-5883	1074	12	g.	g.	PROPN
fnp-5883	1074	13	,	,	PUNCT
fnp-5883	1074	14	buckingham	buckingham	PROPN
fnp-5883	1074	15	,	,	PUNCT
fnp-5883	1074	16	j.	j.	PROPN
fnp-5883	1074	17	,	,	PUNCT
fnp-5883	1074	18	pawson	pawson	PROPN
fnp-5883	1074	19	,	,	PUNCT
fnp-5883	1074	20	r.	r.	PROPN
fnp-5883	1074	21	,	,	PUNCT
fnp-5883	1074	22	2013	2013	NUM
fnp-5883	1074	23	.	.	PUNCT
fnp-5883	1074	24	rameses	ramese	VERB
fnp-5883	1074	25	publication	publication	NOUN
fnp-5883	1074	26	standards	standard	NOUN
fnp-5883	1074	27	:	:	PUNCT
fnp-5883	1074	28	realist	realist	ADJ
fnp-5883	1074	29	syntheses	synthesis	NOUN
fnp-5883	1074	30	.	.	PUNCT
fnp-5883	1075	1	bmc	bmc	PROPN
fnp-5883	1075	2	med	med	PROPN
fnp-5883	1075	3	.	.	PROPN
fnp-5883	1076	1	11	11	NUM
fnp-5883	1076	2	,	,	PUNCT
fnp-5883	1076	3	21	21	NUM
fnp-5883	1076	4	.	.	PUNCT
fnp-5883	1077	1	https://doi.org/10.1186/1741-7015-11-21	https://doi.org/10.1186/1741-7015-11-21	PROPN
fnp-5883	1077	2	woods	wood	NOUN
fnp-5883	1077	3	,	,	PUNCT
fnp-5883	1077	4	e.j	e.j	PROPN
fnp-5883	1077	5	.	.	PROPN
fnp-5883	1077	6	,	,	PUNCT
fnp-5883	1077	7	mullen	mullen	PROPN
fnp-5883	1077	8	,	,	PUNCT
fnp-5883	1077	9	s.f	s.f	PROPN
fnp-5883	1077	10	.	.	PROPN
fnp-5883	1077	11	,	,	PUNCT
fnp-5883	1077	12	2016	2016	NUM
fnp-5883	1077	13	.	.	PUNCT
fnp-5883	1078	1	organ	organ	NOUN
fnp-5883	1078	2	preservation	preservation	NOUN
fnp-5883	1078	3	:	:	PUNCT
fnp-5883	1078	4	cryobiology	cryobiology	NOUN
fnp-5883	1078	5	and	and	CCONJ
fnp-5883	1078	6	beyond	beyond	ADP
fnp-5883	1078	7	.	.	PUNCT
fnp-5883	1079	1	curr	curr	PROPN
fnp-5883	1079	2	.	.	PUNCT
fnp-5883	1079	3	stem	stem	NOUN
fnp-5883	1079	4	cell	cell	NOUN
fnp-5883	1079	5	rep	rep	PROPN
fnp-5883	1079	6	.	.	PROPN
fnp-5883	1079	7	2	2	NUM
fnp-5883	1079	8	,	,	PUNCT
fnp-5883	1079	9	104–117	104–117	NUM
fnp-5883	1079	10	.	.	PUNCT
fnp-5883	1080	1	https://doi.org/10.1007/s40778-016-0042-8	https://doi.org/10.1007/s40778-016-0042-8	PROPN
fnp-5883	1080	2	wowk	wowk	PROPN
fnp-5883	1080	3	,	,	PUNCT
fnp-5883	1080	4	b.	b.	PROPN
fnp-5883	1080	5	,	,	PUNCT
fnp-5883	1080	6	2010	2010	NUM
fnp-5883	1080	7	.	.	PUNCT
fnp-5883	1081	1	thermodynamic	thermodynamic	ADJ
fnp-5883	1081	2	aspects	aspect	NOUN
fnp-5883	1081	3	of	of	ADP
fnp-5883	1081	4	vitrification	vitrification	NOUN
fnp-5883	1081	5	.	.	PUNCT
fnp-5883	1082	1	cryobiology	cryobiology	NOUN
fnp-5883	1082	2	60	60	NUM
fnp-5883	1082	3	,	,	PUNCT
fnp-5883	1082	4	11–22	11–22	NUM
fnp-5883	1082	5	.	.	PUNCT
fnp-5883	1083	1	https://doi.org/10.1016/j.cryobiol.2009.05.007	https://doi.org/10.1016/j.cryobiol.2009.05.007	VERB
fnp-5883	1083	2	wowk	wowk	NOUN
fnp-5883	1083	3	,	,	PUNCT
fnp-5883	1083	4	b.	b.	PROPN
fnp-5883	1083	5	,	,	PUNCT
fnp-5883	1083	6	phan	phan	PROPN
fnp-5883	1083	7	,	,	PUNCT
fnp-5883	1083	8	j.	j.	PROPN
fnp-5883	1083	9	,	,	PUNCT
fnp-5883	1083	10	pagotan	pagotan	PROPN
fnp-5883	1083	11	,	,	PUNCT
fnp-5883	1083	12	r.	r.	PROPN
fnp-5883	1083	13	,	,	PUNCT
fnp-5883	1083	14	galvez	galvez	PROPN
fnp-5883	1083	15	,	,	PUNCT
fnp-5883	1083	16	e.	e.	PROPN
fnp-5883	1083	17	,	,	PUNCT
fnp-5883	1083	18	fahy	fahy	PROPN
fnp-5883	1083	19	,	,	PUNCT
fnp-5883	1083	20	g.m	g.m	PROPN
fnp-5883	1083	21	.	.	PROPN
fnp-5883	1083	22	,	,	PUNCT
fnp-5883	1083	23	2024	2024	NUM
fnp-5883	1083	24	.	.	PUNCT
fnp-5883	1084	1	27	27	NUM
fnp-5883	1084	2	mhz	mhz	ADJ
fnp-5883	1084	3	constant	constant	ADJ
fnp-5883	1084	4	field	field	NOUN
fnp-5883	1084	5	dielectric	dielectric	ADJ
fnp-5883	1084	6	warming	warming	NOUN
fnp-5883	1084	7	of	of	ADP
fnp-5883	1084	8	kidneys	kidney	NOUN
fnp-5883	1084	9	cryopreserved	cryopreserve	VERB
fnp-5883	1084	10	by	by	ADP
fnp-5883	1084	11	vitrification	vitrification	NOUN
fnp-5883	1084	12	.	.	PUNCT
fnp-5883	1085	1	cryobiology	cryobiology	NOUN
fnp-5883	1085	2	104893	104893	NUM
fnp-5883	1085	3	.	.	PUNCT
fnp-5883	1086	1	https://doi.org/10.1016/j.cryobiol.2024.104893	https://doi.org/10.1016/j.cryobiol.2024.104893	ADJ
fnp-5883	1086	2	zennoune	zennoune	NOUN
fnp-5883	1086	3	,	,	PUNCT
fnp-5883	1086	4	a.	a.	NOUN
fnp-5883	1086	5	,	,	PUNCT
fnp-5883	1086	6	latil	latil	NOUN
fnp-5883	1086	7	,	,	PUNCT
fnp-5883	1086	8	p.	p.	PROPN
fnp-5883	1086	9	,	,	PUNCT
fnp-5883	1086	10	flin	flin	PROPN
fnp-5883	1086	11	,	,	PUNCT
fnp-5883	1086	12	f.	f.	PROPN
fnp-5883	1086	13	,	,	PUNCT
fnp-5883	1086	14	perrin	perrin	PROPN
fnp-5883	1086	15	,	,	PUNCT
fnp-5883	1086	16	j.	j.	PROPN
fnp-5883	1086	17	,	,	PUNCT
fnp-5883	1086	18	weitkamp	weitkamp	PROPN
fnp-5883	1086	19	,	,	PUNCT
fnp-5883	1086	20	t.	t.	PROPN
fnp-5883	1086	21	,	,	PUNCT
fnp-5883	1086	22	scheel	scheel	PROPN
fnp-5883	1086	23	,	,	PUNCT
fnp-5883	1086	24	m.	m.	NOUN
fnp-5883	1086	25	,	,	PUNCT
fnp-5883	1086	26	geindreau	geindreau	PROPN
fnp-5883	1086	27	,	,	PUNCT
fnp-5883	1086	28	c.	c.	PROPN
fnp-5883	1086	29	,	,	PUNCT
fnp-5883	1086	30	benkhelifa	benkhelifa	PROPN
fnp-5883	1086	31	,	,	PUNCT
fnp-5883	1086	32	h.	h.	PROPN
fnp-5883	1086	33	,	,	PUNCT
fnp-5883	1086	34	ndoye	ndoye	NOUN
fnp-5883	1086	35	,	,	PUNCT
fnp-5883	1086	36	f.-t	f.-t	NOUN
fnp-5883	1086	37	.	.	PUNCT
fnp-5883	1086	38	,	,	PUNCT
fnp-5883	1086	39	2022	2022	NUM
fnp-5883	1086	40	.	.	PUNCT
fnp-5883	1087	1	investigating	investigate	VERB
fnp-5883	1087	2	the	the	DET
fnp-5883	1087	3	influence	influence	NOUN
fnp-5883	1087	4	of	of	ADP
fnp-5883	1087	5	freezing	freeze	VERB
fnp-5883	1087	6	rate	rate	NOUN
fnp-5883	1087	7	and	and	CCONJ
fnp-5883	1087	8	frozen	frozen	ADJ
fnp-5883	1087	9	storage	storage	NOUN
fnp-5883	1087	10	conditions	condition	NOUN
fnp-5883	1087	11	on	on	ADP
fnp-5883	1087	12	a	a	DET
fnp-5883	1087	13	model	model	NOUN
fnp-5883	1087	14	sponge	sponge	NOUN
fnp-5883	1087	15	cake	cake	NOUN
fnp-5883	1087	16	using	use	VERB
fnp-5883	1087	17	synchrotron	synchrotron	NOUN
fnp-5883	1087	18	x	x	NOUN
fnp-5883	1087	19	-	-	NOUN
fnp-5883	1087	20	rays	ray	NOUN
fnp-5883	1087	21	micro	micro	ADJ
fnp-5883	1087	22	-	-	ADJ
fnp-5883	1087	23	computed	computed	ADJ
fnp-5883	1087	24	tomography	tomography	NOUN
fnp-5883	1087	25	.	.	PUNCT
fnp-5883	1088	1	food	food	NOUN
fnp-5883	1088	2	res	re	NOUN
fnp-5883	1088	3	.	.	PUNCT
fnp-5883	1089	1	int	int	NOUN
fnp-5883	1089	2	.	.	PUNCT
fnp-5883	1090	1	ott	ott	PROPN
fnp-5883	1090	2	.	.	PUNCT
fnp-5883	1091	1	ont	ont	PROPN
fnp-5883	1091	2	162	162	NUM
fnp-5883	1091	3	,	,	PUNCT
fnp-5883	1091	4	112116	112116	NUM
fnp-5883	1091	5	.	.	PUNCT
fnp-5883	1092	1	https://doi.org/10.1016/j.foodres.2022.112116	https://doi.org/10.1016/j.foodres.2022.112116	PROPN
fnp-5883	1092	2	zhang	zhang	PROPN
fnp-5883	1092	3	,	,	PUNCT
fnp-5883	1092	4	m.	m.	NOUN
fnp-5883	1092	5	,	,	PUNCT
fnp-5883	1092	6	oldenhof	oldenhof	ADV
fnp-5883	1092	7	,	,	PUNCT
fnp-5883	1092	8	h.	h.	PROPN
fnp-5883	1092	9	,	,	PUNCT
fnp-5883	1092	10	sieme	sieme	PROPN
fnp-5883	1092	11	,	,	PUNCT
fnp-5883	1092	12	h.	h.	PROPN
fnp-5883	1092	13	,	,	PUNCT
fnp-5883	1092	14	wolkers	wolker	NOUN
fnp-5883	1092	15	,	,	PUNCT
fnp-5883	1092	16	w.f	w.f	PROPN
fnp-5883	1092	17	.	.	PROPN
fnp-5883	1092	18	,	,	PUNCT
fnp-5883	1092	19	2016	2016	NUM
fnp-5883	1092	20	.	.	PUNCT
fnp-5883	1093	1	freezing	freeze	VERB
fnp-5883	1093	2	-	-	PUNCT
fnp-5883	1093	3	induced	induced	ADJ
fnp-5883	1093	4	uptake	uptake	NOUN
fnp-5883	1093	5	of	of	ADP
fnp-5883	1093	6	trehalose	trehalose	NOUN
fnp-5883	1093	7	into	into	ADP
fnp-5883	1093	8	mammalian	mammalian	ADJ
fnp-5883	1093	9	cells	cell	NOUN
fnp-5883	1093	10	facilitates	facilitate	VERB
fnp-5883	1093	11	cryopreservation	cryopreservation	NOUN
fnp-5883	1093	12	.	.	PUNCT
fnp-5883	1094	1	biochim	biochim	NOUN
fnp-5883	1094	2	.	.	PUNCT
fnp-5883	1095	1	biophys	biophy	NOUN
fnp-5883	1095	2	.	.	PUNCT
fnp-5883	1096	1	acta	acta	PROPN
fnp-5883	1096	2	1858	1858	NUM
fnp-5883	1096	3	,	,	PUNCT
fnp-5883	1096	4	1400–1409	1400–1409	NUM
fnp-5883	1096	5	.	.	PUNCT
fnp-5883	1097	1	https://doi.org/10.1016/j.bbamem.2016.03.020	https://doi.org/10.1016/j.bbamem.2016.03.020	PROPN
fnp-5883	1097	2	zhao	zhao	PROPN
fnp-5883	1097	3	,	,	PUNCT
fnp-5883	1097	4	s.	s.	PROPN
fnp-5883	1097	5	,	,	PUNCT
fnp-5883	1097	6	studer	studer	NOUN
fnp-5883	1097	7	,	,	PUNCT
fnp-5883	1097	8	d.	d.	PROPN
fnp-5883	1097	9	,	,	PUNCT
fnp-5883	1097	10	graber	graber	PROPN
fnp-5883	1097	11	,	,	PUNCT
fnp-5883	1097	12	w.	w.	PROPN
fnp-5883	1097	13	,	,	PUNCT
fnp-5883	1097	14	nestel	nestel	PROPN
fnp-5883	1097	15	,	,	PUNCT
fnp-5883	1097	16	s.	s.	PROPN
fnp-5883	1097	17	,	,	PUNCT
fnp-5883	1097	18	frotscher	frotscher	ADJ
fnp-5883	1097	19	,	,	PUNCT
fnp-5883	1097	20	m.	m.	NOUN
fnp-5883	1097	21	,	,	PUNCT
fnp-5883	1097	22	2012	2012	NUM
fnp-5883	1097	23	.	.	PUNCT
fnp-5883	1098	1	fine	fine	ADJ
fnp-5883	1098	2	structure	structure	NOUN
fnp-5883	1098	3	of	of	ADP
fnp-5883	1098	4	hippocampal	hippocampal	ADJ
fnp-5883	1098	5	mossy	mossy	NOUN
fnp-5883	1098	6	fiber	fiber	NOUN
fnp-5883	1098	7	synapses	synapsis	NOUN
fnp-5883	1098	8	following	follow	VERB
fnp-5883	1098	9	rapid	rapid	ADJ
fnp-5883	1098	10	high	high	ADJ
fnp-5883	1098	11	-	-	PUNCT
fnp-5883	1098	12	pressure	pressure	NOUN
fnp-5883	1098	13	freezing	freezing	NOUN
fnp-5883	1098	14	.	.	PUNCT
fnp-5883	1099	1	epilepsia	epilepsia	NOUN
fnp-5883	1099	2	53	53	NUM
fnp-5883	1099	3	suppl	suppl	ADJ
fnp-5883	1099	4	1	1	NUM
fnp-5883	1099	5	,	,	PUNCT
fnp-5883	1099	6	4–8	4–8	NOUN
fnp-5883	1099	7	.	.	PUNCT
fnp-5883	1100	1	https://doi.org/10.1111/j.1528-1167.2012.03469.x	https://doi.org/10.1111/j.1528-1167.2012.03469.x	PROPN
fnp-5883	1100	2	zilles	zilles	PROPN
fnp-5883	1100	3	,	,	PUNCT
fnp-5883	1100	4	k.	k.	PROPN
fnp-5883	1100	5	,	,	PUNCT
fnp-5883	1100	6	schleicher	schleicher	PROPN
fnp-5883	1100	7	,	,	PUNCT
fnp-5883	1100	8	a.	a.	NOUN
fnp-5883	1100	9	,	,	PUNCT
fnp-5883	1100	10	palomero	palomero	PROPN
fnp-5883	1100	11	-	-	PUNCT
fnp-5883	1100	12	gallagher	gallagher	PROPN
fnp-5883	1100	13	,	,	PUNCT
fnp-5883	1100	14	n.	n.	NOUN
fnp-5883	1100	15	,	,	PUNCT
fnp-5883	1100	16	amunts	amunt	NOUN
fnp-5883	1100	17	,	,	PUNCT
fnp-5883	1100	18	k.	k.	PROPN
fnp-5883	1100	19	,	,	PUNCT
fnp-5883	1100	20	2002	2002	NUM
fnp-5883	1100	21	.	.	PUNCT
fnp-5883	1101	1	21	21	NUM
fnp-5883	1101	2	quantitative	quantitative	ADJ
fnp-5883	1101	3	analysis	analysis	NOUN
fnp-5883	1101	4	of	of	ADP
fnp-5883	1101	5	cytoand	cytoand	NOUN
fnp-5883	1101	6	receptor	receptor	NOUN
fnp-5883	1101	7	architecture	architecture	NOUN
fnp-5883	1101	8	of	of	ADP
fnp-5883	1101	9	the	the	DET
fnp-5883	1101	10	human	human	ADJ
fnp-5883	1101	11	brain	brain	NOUN
fnp-5883	1101	12	,	,	PUNCT
fnp-5883	1101	13	in	in	ADP
fnp-5883	1101	14	:	:	PUNCT
fnp-5883	1101	15	toga	toga	PROPN
fnp-5883	1101	16	,	,	PUNCT
fnp-5883	1101	17	a.w	a.w	PROPN
fnp-5883	1101	18	.	.	PROPN
fnp-5883	1101	19	,	,	PUNCT
fnp-5883	1101	20	mazziotta	mazziotta	PROPN
fnp-5883	1101	21	,	,	PUNCT
fnp-5883	1101	22	j.c	j.c	PROPN
fnp-5883	1101	23	.	.	PROPN
fnp-5883	1101	24	(	(	PUNCT
fnp-5883	1101	25	eds	ed	NOUN
fnp-5883	1101	26	.	.	PUNCT
fnp-5883	1101	27	)	)	PUNCT
fnp-5883	1101	28	,	,	PUNCT
fnp-5883	1101	29	brain	brain	NOUN
fnp-5883	1101	30	mapping	mapping	NOUN
fnp-5883	1101	31	:	:	PUNCT
fnp-5883	1101	32	the	the	DET
fnp-5883	1101	33	methods	method	NOUN
fnp-5883	1101	34	(	(	PUNCT
fnp-5883	1101	35	second	second	ADJ
fnp-5883	1101	36	edition	edition	NOUN
fnp-5883	1101	37	)	)	PUNCT
fnp-5883	1101	38	.	.	PUNCT
fnp-5883	1102	1	academic	academic	ADJ
fnp-5883	1102	2	press	press	NOUN
fnp-5883	1102	3	,	,	PUNCT
fnp-5883	1102	4	san	san	PROPN
fnp-5883	1102	5	diego	diego	PROPN
fnp-5883	1102	6	,	,	PUNCT
fnp-5883	1102	7	pp	pp	X
fnp-5883	1102	8	.	.	PUNCT
fnp-5883	1103	1	573–602	573–602	NUM
fnp-5883	1103	2	.	.	PUNCT
fnp-5883	1104	1	https://doi.org/10.1016/b978-012693019-1/50023-x	https://doi.org/10.1016/b978-012693019-1/50023-x	PROPN
fnp-5883	1104	2	zuber	zuber	PROPN
fnp-5883	1104	3	,	,	PUNCT
fnp-5883	1104	4	b.	b.	PROPN
fnp-5883	1104	5	,	,	PUNCT
fnp-5883	1104	6	nikonenko	nikonenko	PROPN
fnp-5883	1104	7	,	,	PUNCT
fnp-5883	1104	8	i.	i.	PROPN
fnp-5883	1104	9	,	,	PUNCT
fnp-5883	1104	10	klauser	klauser	PROPN
fnp-5883	1104	11	,	,	PUNCT
fnp-5883	1104	12	p.	p.	PROPN
fnp-5883	1104	13	,	,	PUNCT
fnp-5883	1104	14	muller	muller	PROPN
fnp-5883	1104	15	,	,	PUNCT
fnp-5883	1104	16	d.	d.	PROPN
fnp-5883	1104	17	,	,	PUNCT
fnp-5883	1104	18	dubochet	dubochet	PROPN
fnp-5883	1104	19	,	,	PUNCT
fnp-5883	1104	20	j.	j.	PROPN
fnp-5883	1104	21	,	,	PUNCT
fnp-5883	1104	22	2005	2005	NUM
fnp-5883	1104	23	.	.	PUNCT
fnp-5883	1105	1	the	the	DET
fnp-5883	1105	2	mammalian	mammalian	ADJ
fnp-5883	1105	3	central	central	ADJ
fnp-5883	1105	4	nervous	nervous	ADJ
fnp-5883	1105	5	synaptic	synaptic	ADJ
fnp-5883	1105	6	cleft	cleft	NOUN
fnp-5883	1105	7	contains	contain	VERB
fnp-5883	1105	8	a	a	DET
fnp-5883	1105	9	high	high	ADJ
fnp-5883	1105	10	density	density	NOUN
fnp-5883	1105	11	of	of	ADP
fnp-5883	1105	12	periodically	periodically	ADV
fnp-5883	1105	13	organized	organize	VERB
fnp-5883	1105	14	complexes	complex	NOUN
fnp-5883	1105	15	.	.	PUNCT
fnp-5883	1106	1	proc	proc	NOUN
fnp-5883	1106	2	.	.	PUNCT
fnp-5883	1107	1	natl	natl	PROPN
fnp-5883	1107	2	.	.	PUNCT
fnp-5883	1108	1	acad	acad	PROPN
fnp-5883	1108	2	.	.	PUNCT
fnp-5883	1109	1	sci	sci	PROPN
fnp-5883	1109	2	.	.	PROPN
fnp-5883	1110	1	102	102	NUM
fnp-5883	1110	2	,	,	PUNCT
fnp-5883	1110	3	19192–19197	19192–19197	NUM
fnp-5883	1110	4	.	.	PUNCT
fnp-5883	1111	1	https://doi.org/10.1073/pnas.0509527102	https://doi.org/10.1073/pnas.0509527102	PROPN
fnp-5883	1111	2	copyright	copyright	NOUN
fnp-5883	1111	3	:	:	PUNCT
fnp-5883	1112	1	©	©	PROPN
fnp-5883	1112	2	2024	2024	NUM
fnp-5883	1112	3	the	the	DET
fnp-5883	1112	4	author(s	author(s	NOUN
fnp-5883	1112	5	)	)	PUNCT
fnp-5883	1112	6	.	.	PUNCT
fnp-5883	1113	1	this	this	PRON
fnp-5883	1113	2	is	be	AUX
fnp-5883	1113	3	an	an	DET
fnp-5883	1113	4	open	open	ADJ
fnp-5883	1113	5	access	access	NOUN
fnp-5883	1113	6	article	article	NOUN
fnp-5883	1113	7	distributed	distribute	VERB
fnp-5883	1113	8	under	under	ADP
fnp-5883	1113	9	the	the	DET
fnp-5883	1113	10	terms	term	NOUN
fnp-5883	1113	11	of	of	ADP
fnp-5883	1113	12	the	the	DET
fnp-5883	1113	13	creative	creative	ADJ
fnp-5883	1113	14	commons	common	NOUN
fnp-5883	1113	15	attribution	attribution	NOUN
fnp-5883	1113	16	4.0	4.0	NUM
fnp-5883	1113	17	international	international	ADJ
fnp-5883	1113	18	license	license	NOUN
fnp-5883	1113	19	(	(	PUNCT
fnp-5883	1113	20	https://creativecommons.org/licenses/by/4.0/	https://creativecommons.org/licenses/by/4.0/	PROPN
fnp-5883	1113	21	)	)	PUNCT
fnp-5883	1113	22	,	,	PUNCT
fnp-5883	1113	23	which	which	PRON
fnp-5883	1113	24	permits	permit	VERB
fnp-5883	1113	25	unrestricted	unrestricted	ADJ
fnp-5883	1113	26	use	use	NOUN
fnp-5883	1113	27	,	,	PUNCT
fnp-5883	1113	28	distribution	distribution	NOUN
fnp-5883	1113	29	,	,	PUNCT
fnp-5883	1113	30	and	and	CCONJ
fnp-5883	1113	31	reproduction	reproduction	NOUN
fnp-5883	1113	32	in	in	ADP
fnp-5883	1113	33	any	any	DET
fnp-5883	1113	34	medium	medium	NOUN
fnp-5883	1113	35	,	,	PUNCT
fnp-5883	1113	36	provided	provide	VERB
fnp-5883	1113	37	the	the	DET
fnp-5883	1113	38	original	original	ADJ
fnp-5883	1113	39	author	author	NOUN
fnp-5883	1113	40	and	and	CCONJ
fnp-5883	1113	41	source	source	NOUN
fnp-5883	1113	42	are	be	AUX
fnp-5883	1113	43	credited	credit	VERB
fnp-5883	1113	44	,	,	PUNCT
fnp-5883	1113	45	a	a	DET
fnp-5883	1113	46	link	link	NOUN
fnp-5883	1113	47	to	to	ADP
fnp-5883	1113	48	the	the	DET
fnp-5883	1113	49	creative	creative	ADJ
fnp-5883	1113	50	commons	common	NOUN
fnp-5883	1113	51	license	license	NOUN
fnp-5883	1113	52	is	be	AUX
fnp-5883	1113	53	provided	provide	VERB
fnp-5883	1113	54	,	,	PUNCT
fnp-5883	1113	55	and	and	CCONJ
fnp-5883	1113	56	any	any	DET
fnp-5883	1113	57	changes	change	NOUN
fnp-5883	1113	58	are	be	AUX
fnp-5883	1113	59	indicated	indicate	VERB
fnp-5883	1113	60	.	.	PUNCT
fnp-5883	1114	1	the	the	DET
fnp-5883	1114	2	creative	creative	ADJ
fnp-5883	1114	3	commons	common	NOUN
fnp-5883	1114	4	public	public	ADJ
fnp-5883	1114	5	domain	domain	NOUN
fnp-5883	1114	6	dedication	dedication	NOUN
fnp-5883	1114	7	waiver	waiver	NOUN
fnp-5883	1114	8	(	(	PUNCT
fnp-5883	1114	9	https://creativecommons.org/publicdomain/zero/1.0/	https://creativecommons.org/publicdomain/zero/1.0/	PROPN
fnp-5883	1114	10	)	)	PUNCT
fnp-5883	1114	11	applies	apply	VERB
fnp-5883	1114	12	to	to	ADP
fnp-5883	1114	13	the	the	DET
fnp-5883	1114	14	data	datum	NOUN
fnp-5883	1114	15	made	make	VERB
fnp-5883	1114	16	available	available	ADJ
fnp-5883	1114	17	in	in	ADP
fnp-5883	1114	18	this	this	DET
fnp-5883	1114	19	article	article	NOUN
fnp-5883	1114	20	,	,	PUNCT
fnp-5883	1114	21	unless	unless	SCONJ
fnp-5883	1114	22	otherwise	otherwise	ADV
fnp-5883	1114	23	stated	state	VERB
fnp-5883	1114	24	.	.	PUNCT