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							| Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007



	

				
						
 
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																Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007
										

				


				
				
					
					
						
																					
				
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					Vol. 13 No. 2 (2023): February 2023
				
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									Research Articles
							
		



	
		  	 

		
	
		Screening and characterization of antioxidant, anti-aging, andanti-microbial activity of herbal extracts
	


	
	
		

							
					Authors

						
							
								Chakkraphong Khonthun
							
															
									Division of Biochemistry, Faculty of Medical Sciences, University of Phayao.
																	
																																		
	
							
								Krissana Khoothiam
							
															
									Division of Microbiology, School of Medical Sciences, University of Phayao, Phayao, Thailand
																	
																																		
	
							
								Orada Chumphukam
							
															
									Division of Biochemistry, Faculty of Medical Sciences, University of Phayao.
																	
																																		
	
							
								Rungthip Thongboontho
							
															
									Division of Biochemistry, Faculty of Medical Sciences, University of Phayao.
																	
																																		
	
							
								Panida Oonlao
							
															
									Division of Biochemistry, Faculty of Medical Sciences, University of Phayao.
																	
																																		
	
							
								Piyawan Nuntaboon
							
															
									Division of Biochemistry, Faculty of Medical Sciences, University of Phayao.
																	
																																		
	
							
								Kanokkarn Phromnoi
							
															
									Division of Biochemistry, Faculty of Medical Sciences, University of Phayao.
																	
																																				
																		
										https://orcid.org/0000-0002-8003-6112
									
								
													


				
			
																	
					
												DOI:
					

					
						
							https://doi.org/10.31989/ffhd.v13i2.1070
						
					
				
			

						
										
					Abstract

					Background: Aging is a process caused by oxidants and aging-related enzymes. Therefore, the inhibition of these processes can exacerbate anti-aging agents. This study aimed to evaluate the antioxidant and anti-aging activities of leaf extracts from seven herbs used in traditional Thai herbal remedies. 
Methods: Researchers assessed the total levels of phenolic content (TPC), antioxidant, anti-collagenase, and anti-tyrosinase activity using colorimetric methods. Cytotoxicity effects were determined using MTT assays, and matrix metalloproteinases(MMPs) secretion was assessed through gelatin zymography. In addition, inhibitory effects on the growth of microorganisms were examined using the disc diffusion and broth microdilution method.
Results: TPC ranged between 48.68–440.91 mg GAE/g of ethanolic leaf extracts. High antioxidant activities against ABTS radicals were detected in P. granatum, P. emblica, P. guajava, T. bellirica, and T. chebula, while high DPPH neutralization appeared in M. coreia, P. guajava, and P. granatum. FRAP assays significantly reduced the power of T. chebula and P. granatum. T. chebula and P. guajava exhibited the highest inhibitory effect on H2O2induced ROS production. P. emblica, P. guajava, T. bellirica, and E. hygrophilus reduced tyrosinase and collagenase activities.  P. gaujava, T. chebula, and T. bellirica were shown to inhibit the secretion of MMP-2 from fibroblast cells. All concentrations of leaf extracts were non-toxic to fibroblast cells. P.granatum and T. bellirica could inhibit the growth of P. acnes, E. coli, and S. aureus. 
Conclusion: Preliminary studies showed that P. granatum and T. bellirica leaf extracts have antioxidants, anti-aging, and anti-bacterial activitiesand have potential for use as active ingredients for the development of functional foods and cosmetic products.

Keywords: antioxidants, anti-aging, leaf extracts


				
			
			

						
																																																																																												
					
													Author Biographies
											

						
								
																																							Krissana Khoothiam,  Division of Microbiology, School of Medical Sciences, University of Phayao, Phayao, Thailand 
																	

								
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																																							Orada Chumphukam,  Division of Biochemistry, Faculty of Medical Sciences, University of Phayao. 
																	

								
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																																							Kanokkarn Phromnoi,  Division of Biochemistry, Faculty of Medical Sciences, University of Phayao. 
																	

								
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						Published
					

					
																			2023-02-27
																	

				
							

			
						
										

											
							
								Issue
							

							
								
									Vol. 13 No. 2 (2023): February 2023
								
							

						
					
											
							
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								Research Articles
							

						
					
									

			
						
										
					
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