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							| Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007



	

				
						
 
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																Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007
										

				


				
				
					
					
						
																					
				
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					Vol. 1 No. 10 (2011): October 2011
				
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									Research Articles
							
		



	
		  	 

		
	
		Fermentation by gut microbiota cultured in a simulator of the human intestinal microbial ecosystem is improved by probiotic Enterococcus faecium CRL 183
	


	
	
		

							
					Authors

						
							
								Katia Sivieri
							
															
									Department of Food and Nutrition, Laboratory Study of Probiotics and Prebiotics, Faculty of Pharmaceutical Sciences of Araraquara, UNESP-Paulista State University, Araraquara, São Paulo
																	
																																		
	
							
								Fernanda Bianchi
							
															
									Department of Food and Nutrition, Laboratory Study of Probiotics and Prebiotics, Faculty of Pharmaceutical Sciences of Araraquara, UNESP-Paulista State University, Araraquara, São Paulo
																	
																																		
	
							
								Maria A. Tallarico
							
															
									School of Engineering of São Carlos, University of São Paulo, Sao Paulo
																	
																																		
	
							
								Elizeu A. Rossi
							
															
									Department of Food and Nutrition, Laboratory Study of Probiotics and Prebiotics, Faculty of Pharmaceutical Sciences of Araraquara, UNESP-Paulista State University, Araraquara, São Paulo
																	
																																		


				
			
																	
					
												DOI:
					

					
						
							https://doi.org/10.31989/ffhd.v1i10.119
						
					
				
			

						
										
					Abstract

					Background: Enterococci are used in a large number of dairy products, such as starter cultures in food supplements and in foods considered functional. In vitro gut fermentation models present an unmatched opportunity of performing studies frequently allenged in humans and animals owing to ethical concerns. A dynamic model of the human intestinal microbial ecosystem (SHIME) was designed to better simulate conditions intestinal microbiota.
Methods: The SHIME model was used to study the effect of Enterococuus faecium CRL 183 on the fermentation pattern of the colon microbiota. Initially, an inoculum prepared from human feces was introduced into the reactor vessels and stabilized over 2 wk using a culture medium. This stabilization period was followed by a 2-wk control period during which the microbiota were monitored. The microbiota were then subjected to a 4-wk treatment period by adding 108 CFU/mL of the Enterococcus faecium CRL 183 to vessel one (the stomach compartment).
Results: The addition resulted into an overall increase of bacterial marker populations (Enterobacteriaceae, Lactobacillus spp., Bifidobacterium spp. and Clostridium spp.), with a significant increase of the Lactobacillus sp. and Bifidobacterium sp populations. The short-chain fatty acid (SCFA) concentration increased during the supplementation period; this was due mainly to a significant increase in the levels of acetic, butyric and propionic acids. Ammonium concentrations increased during the supplementation period.
Conclusions: Results showed that the major effect of E. faecium CRL 183 was found in the ascendant and transverse colon. 
Key words: Gut microbiota, Enterococcus, Gastrointestinal resource management, Simulator of Human Intestinal Microbial Ecosystem (SHIME)

				
			
			

						
																																								
						
		


		

						
										
					
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																			2011-10-08
																	

				
							

			
						
										

											
							
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									Vol. 1 No. 10 (2011): October 2011
								
							

						
					
											
							
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								Research Articles
							

						
					
									

			
						
										
					
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