






















































Regetting-18 complex powder (R-18CP) - A formulation of components from food-medicine and its anti-aging effects
							| Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007



	

				
						
 
	Skip to main content
	Skip to main navigation menu
		Skip to site footer


			

				
					
						Open Menu
					
										
																Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007
										

				


				
				
					
					
						
																					
				
					Home
				
										
									
										Food Science Publisher
									
								
	
									
										Functional Food Center
									
								


							
	
				
					Current
				
							
	
				
					Archives
				
							
	
				
					About
				
										
									
										About the Journal
									
								
	
									
										Aims and Scope
									
								
	
									
										Contact
									
								
	
									
										Editing Services
									
								
	
									
										Editorial Team
									
								
	
									
										Express Peer Review
									
								
	
									
										Graphical Abstract
									
								
	
									
										Journal Insights
									
								
	
									
										Privacy Statement
									
								
	
									
										Submissions
									
								


							
	
				
					Announcements
				
							
	
				
					Search
				
							



				

																						
									
										
										Search
									
								

													

					

					
								
				
					Register
				
							
	
				
					Login
				
							



					

				
			

		

						
			
				


			
		
			
				Home
			
			/
		
	
			
				Archives
			
			/
		
	
				
					Vol. 15 No. 7 (2025): July 2025
				
				/
			
	
			
									Research Articles
							
		



	
		  	 

		
	
		Regetting-18 complex powder (R-18CP) - A formulation of components from food-medicine and its anti-aging effects
	


	
	
		

							
					Authors

						
							
								Paul F. Davis
							
																																		
	
							
								Lan Yuan
							
																																		


				
			
																	
					
												DOI:
					

					
						
							https://doi.org/10.31989/ffhd.v15i7.1647
						
					
				
			

						
										
					Abstract

					Background: Aging is a natural process that affects all living beings. This process leads to various physiological and pathological changes. Human health has traditionally relied on a combination of dietary supplements and herbal remedies (food-medicine homogeneity) to combat the effects of aging. Recent scientific advancements have provided more profound insights into the mechanisms by which these traditional substances contribute to the anti-aging impacts through their active components. 

Objectives: A formulation called Regetting-18 Complex Powder (R-18CP) was investigated using multiple aging markers that were hypothesized to inhibit cellular senescence. This formulation included several bioactive ingredients known for their anti-aging properties, such as Panax ginseng, Ganoderma lucidum, and Polygonati rhizome.

Methods: Adult male and female zebrafish were paired overnight, and embryos were collected the following day. Six experimental groups were established, each consisting of 10 fish, and all groups were conducted in triplicate. The groups included: (1) Control, (2) Model, (3) Positive control (treated with 5 μg/mL resveratrol), and (4) three R-18CP treatment groups (treated with 1 μg/mL, 0.5 μg/mL, and 0.1 μg/mL, respectively). At 72 hours post-fertilization, various assays were performed: 10 fish per group were stained for β-galactosidase, another 10 for reactive oxygen species (ROS), 10 were analyzed for mitochondrial membrane potential, 50 were used to assess telomerase levels, and DNA was extracted from 30 fish for telomere length analysis.

Results: R-18CP reduced β-galactosidase staining intensity in a dose-dependent manner. Increasing concentrations of R-18CP were associated with decreased ROS staining intensity, elevated telomerase levels, and longer telomeres. Additionally, the mitochondrial membrane potential staining intensity increased in proportion to the R-18CP concentration.

Conclusion: Based on the five distinct aging markers assessed in the zebrafish model, R-18CP may be able to restrict aging. Further studies using human models are recommended and necessary. 

Novelty of the Study: This study is the first to report that an R-18CP can exert anti-aging effects through telomere protection and improved mitochondrial function in zebrafish models. This suggests R-18CP is a potential therapeutic compound for anti-aging.

Keywords: anti-aging, food-medicine homogeneity, zebrafish, β-galactosidase, telomere, telomerase, reactive oxygen, membrane potential 





				
			
			

						
																										
						
		


		

						
										
					
						Downloads
					

						
								
	
													


		
	[Abstract]

	
							
	
								
	
													


		
	[Full Article]

	
							


				

						
						
				
					
						Published
					

					
																			2025-07-08
																	

				
							

			
						
										

											
							
								Issue
							

							
								
									Vol. 15 No. 7 (2025): July 2025
								
							

						
					
											
							
								Section
							

							
								Research Articles
							

						
					
									

			
						
										
					
						License
					

																										Copyright (c) 2025 Functional Foods in Health and Disease

														This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

																Authors retain the copyright of their articles and grant the Functional Food Center (FFC) and its journals the right of first publication under the terms of the Creative Commons Attribution 4.0 International License.

This license permits unrestricted use, distribution, and reproduction in any medium, including commercial use, provided the original author(s) and source are properly credited. Authors may post and share their published work freely, provided that the original publication in this journal is acknowledged.

By submitting to this journal, authors confirm that their manuscripts are original, not under consideration elsewhere, and that they hold the necessary rights to grant this license. The Functional Food Center encourages open scientific exchange and allows derivative and extended works, provided attribution to the original publication is maintained.

				

			
			

		

	




	




	


							



	

	

					
				About the Journal      Editing Services      Graphical Abstract      Journal Insights        Submissions        Editorial Team       Privacy Statement        Contact        Current        Archives

 

			

		
		Open Journal Systems Hosting and Support by: OpenJournalSystems.com


	










