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							| Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007



	

				
						
 
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																Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007
										

				


				
				
					
					
						
																					
				
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					Vol. 3 No. 3 (2013): March 2013
				
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									Research Articles
							
		



	
		  	 

		
	
		Activation of the intrinsic-apoptotic pathway in LNCaP prostate cancer cells by genistein- topotecan combination treatments
	


	
	
		

							
					Authors

						
							
								Vanessa Hörmann
							
															
									Department of Biological Sciences, Florida Atlantic University, Davie FL 33314
																	
																																		
	
							
								Sivanesan Dhandayuthapani
							
															
									Rumbaugh Goodwin Institute for Cancer Research, Nova Southeastern University, Plantation FL, 33322
																	
																																		
	
							
								James Kumi-Diaka
							
															
									Department of Biological Sciences, Florida Atlantic University, Davie FL 33314
																	
																																		
	
							
								Appu Rathinavelu
							
															
									Rumbaugh Goodwin Institute for Cancer Research, Nova Southeastern University, Plantation FL, 33322
																	
																																		


				
			
																	
					
												DOI:
					

					
						
							https://doi.org/10.31989/ffhd.v3i3.64
						
					
				
			

						
										
					Abstract

					Background: Prostate cancer is the second most common cancer in American men. The development of alternative preventative and/or treatment options utilizing a combination of phytochemicals and chemotherapeutic drugs could be an attractive alternative compared to conventional carcinoma treatments. Genistein isoflavone is the primary dietary phytochemical found in soy and has demonstrated anti-tumor activities in LNCaP prostate cancer cells. Topotecan Hydrochloride (Hycamtin) is an FDA-approved chemotherapy for secondary treatment of lung, ovarian and cervical cancers. The purpose of this study was to detail the potential activation of the intrinsic apoptotic pathway in LNCaP prostate cancer cells through genistein-topotecan combination treatments. 

Methods: LNCaP cells were cultured in complete RPMI medium in a monolayer (70-80% confluency) at 37ºC and 5% CO2. Treatment consisted of single and combination groups of genistein and topotecan for 24 hours. The treated cells were assayed for i) growth inhibition through trypan blue exclusion assay and microphotography, ii) classification of cellular death through acridine/ ethidium bromide fluorescent staining, and iii) activation of the intrinsic apoptotic pathway through Jc-1: mitochondrial membrane potential assay, cytochrome c release and Bcl-2 protein expression.

Results: The overall data indicated that genistein-topotecan combination was significantly more efficacious in reducing the prostate carcinoma’s viability compared to the single treatment options. In all treatment groups, cell death occurred primarily through the activation of the intrinsic apoptotic pathway.

Conclusion: The combination of topotecan and genistein has the potential to lead to treatment options with equal therapeutic efficiency as traditional chemo- and radiation therapies, but lower cell cytotoxicity and fewer side effects in patients. 

Key words: topotecan; genistein; intrinsic apoptotic cell death

				
			
			

						
																																								
						
		


		

						
										
					
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																			2013-03-30
																	

				
							

			
						
										

											
							
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									Vol. 3 No. 3 (2013): March 2013
								
							

						
					
											
							
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								Research Articles
							

						
					
									

			
						
										
					
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