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							| Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007



	

				
						
 
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																Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007
										

				


				
				
					
					
						
																					
				
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					Vol. 2 No. 5 (2012): May 2012
				
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									Research Articles
							
		



	
		  	 

		
	
		Therapeutic efficacy of Genistein-Cytoreg® combination in breast cancer cells
	


	
	
		

							
					Authors

						
							
								M. M. Johnson
							
															
									Department of Biological Sciences, Department of Biological Sciences, College of Sciences, Florida Atlantic University at Davie, 3200 College Avenue Davie FL 33314
																	
																																		
	
							
								K. J. Kumi-Diaka
							
															
									Department of Biological Sciences, Department of Biological Sciences, College of Sciences, Florida Atlantic University at Davie, 3200 College Avenue Davie FL 33314
																	
																																		
	
							
								R. Zoeller
							
															
									Department of Exercise and Health Sciences, Florida Atlantic University at Davie 3200 College Avenue Davie FL 33314
																	
																																		
	
							
								B. S. Graves
							
															
									Department of Exercise and Health Sciences, Florida Atlantic University at Davie 3200 College Avenue Davie FL 33314
																	
																																		
	
							
								K. T. Merchant
							
															
									Department of Biological Sciences, Department of Biological Sciences, College of Sciences, Florida Atlantic University at Davie, 3200 College Avenue Davie FL 33314
																	
																																		
	
							
								V. P. Hörmann
							
															
									Department of Biological Sciences, Department of Biological Sciences, College of Sciences, Florida Atlantic University at Davie, 3200 College Avenue Davie FL 33314
																	
																																		
	
							
								M. Hassanhi
							
															
									Department of Blood Bank, University of Zulia, Maracaibo
																	
																																		


				
			
																	
					
												DOI:
					

					
						
							https://doi.org/10.31989/ffhd.v2i5.93
						
					
				
			

						
										
					Abstract

					Background: In spite the heavy investments in therapeutic research breast cancer still impacts the lives of women globally. The projected incidence of new cases of in situ breast cancer in the USA for 2011 is 57,650, with estimated 39,520 deaths. The phytoestrogen, genistein and the synthetic compound, Cytoreg® have been shown to inhibit growth and proliferation in many cancer cell lines. Purpose of the Study: In this study, we investigated the therapeutic efficacy of Cytoreg®-genistein combination on growth inhibition in the MCF-7 human breast cancer cells. 
Method: MCF-7 cells were treated with genistein and Cytoreg® single and combination treatments for 24-48hrs; and post treatment chemosensitivity assessed, using: Trypan Blue exclusion and MTT assays for cell viability, Ethidium bromide/Acridine orange to assess apoptosis induction, and FAM Poly-Caspase binding assay for mechanism of action. 
Results: The overall data indicated dose- and time-dependent cell death in the MCF-cells and apoptosis as the major means of treatment-induced growth inhibition with all the treatment regimens. 
Conclusion: Comparatively, the genistein-Cytoreg® combination treatment was significantly more efficacious in growth inhibition in the MCF cells than either genistein or Cytoreg® alone. Genistein seems to act additively with Cytoreg® in combination treatment-induced apoptosis in MCF-7 cells. The normal human breast epithelial cells were not significantly inhibited by either single or the combination treatments.
Key words: Cytoreg®, Genistein, Combination treatment, MCF- cancer cells, apoptosis

				
			
			

						
																																																													
						
		


		

						
										
					
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																			2012-05-20
																	

				
							

			
						
										

											
							
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									Vol. 2 No. 5 (2012): May 2012
								
							

						
					
											
							
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