Turmeric and Chinese goldthread synergistically inhibit prostate cancer cell proliferation and NF-kB signaling | Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007 Skip to main content Skip to main navigation menu Skip to site footer Open Menu Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007 Home Food Science Publisher Functional Food Center Current Archives About About the Journal Aims and Scope Contact Editing Services Editorial Team Express Peer Review Graphical Abstract Journal Insights Privacy Statement Submissions Announcements Search Search Register Login Home / Archives / Vol. 4 No. 7 (2014): July 2014 / Research Articles Turmeric and Chinese goldthread synergistically inhibit prostate cancer cell proliferation and NF-kB signaling Authors Yi Zhao Department of Nutrition University of Tennessee, Knoxville, TN 37996, J. Jason Collier Department of Nutrition University of Tennessee, Knoxville, TN 37996, E-Chu Huang Department of Nutrition University of Tennessee, Knoxville, TN 37996, Jay Whelan Department of Nutrition, Tennessee Agricultural Experiment Station University of Tennessee, Knoxville, TN 37996, DOI: https://doi.org/10.31989/ffhd.v4i7.1 Abstract Background: Pre-clinical studies using bioactive compounds from botanicals appear to offer some protection against cancer. Research using single bioactives contributes greatly to our understanding of their mechanism of action, but in vitro studies demand concentrations that are higher than achievable in humans (µM). However, maintaining these bioactives in the presence of other compounds originally derived from the food or extract of origin may synergistically lower the bioactive dose so translatability becomes feasible. The objective of this study was to determine if bio-efficacy of phytonutrients can be enhanced when used in combination even at doses that are ineffective for any compound when used in isolation.   Methods: The anti-proliferative and molecular effects of herbs (turmeric and Chinese goldthread) and their bioactives (curcumin and ar-turmerone, berberine and coptisine, respectively) were determined in isolation and in combination. Using CWR22Rv1 and HEK293 cells, cell proliferation (as assessed by the MTT assay) and NF-κB promoter activity (using a luciferase reporter construct) were evaluated and synergy of action was assessed by the Chou-Talalay method utilizing CompuSyn® software.   Results: Turmeric and Chinese goldthread act synergistically (combination index<1) when inhibiting cell proliferation with all cell lines tested. The synergy of action of combinations of companion bioactives from the same herb (i.e., curcumin/ar-turmerone and berberine/coptisine) and bioactives from different herbs (i.e., curcumin/berberine) help to explain why turmeric and Chinese goldthread are more effective than their major bioactives in isolation. At the molecule level, curcumin+ar-turmerone and curcumin+coptisine synergistically attenuated TNFα-stimulated NF-κB promoter activity. Even compounds with poor efficacy become more biologically active in the presence of companion compounds. Importantly, the effects of combining any two bioactives or herbal extracts were highly synergistic at concentrations approaching physiological significance (nanomolar).   Conclusions: These results suggest that bioactives in combination (as plant extracts or isolated compounds) are highly synergistic at the cellular and molecular level at physiologically relevant concentrations. These data help to explain why complex mixtures of botanicals may be more efficacious than their bioactives in isolation. Downloads [Abstract] [Full Article] Published 2014-07-22 Issue Vol. 4 No. 7 (2014): July 2014 Section Research Articles License Authors retain the copyright of their articles and grant the Functional Food Center (FFC) and its journals the right of first publication under the terms of the Creative Commons Attribution 4.0 International License. This license permits unrestricted use, distribution, and reproduction in any medium, including commercial use, provided the original author(s) and source are properly credited. Authors may post and share their published work freely, provided that the original publication in this journal is acknowledged. By submitting to this journal, authors confirm that their manuscripts are original, not under consideration elsewhere, and that they hold the necessary rights to grant this license. The Functional Food Center encourages open scientific exchange and allows derivative and extended works, provided attribution to the original publication is maintained. About the Journal      Editing Services      Graphical Abstract      Journal Insights        Submissions        Editorial Team       Privacy Statement        Contact        Current        Archives   Open Journal Systems Hosting and Support by: OpenJournalSystems.com