Screening and characterization of antioxidant, anti-aging, andanti-microbial activity of herbal extracts | Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007 Skip to main content Skip to main navigation menu Skip to site footer Open Menu Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007 Home Food Science Publisher Functional Food Center Current Archives About About the Journal Aims and Scope Contact Editing Services Editorial Team Express Peer Review Graphical Abstract Journal Insights Privacy Statement Submissions Announcements Search Search Register Login Home / Archives / Vol. 13 No. 2 (2023): February 2023 / Research Articles Screening and characterization of antioxidant, anti-aging, andanti-microbial activity of herbal extracts Authors Chakkraphong Khonthun Division of Biochemistry, Faculty of Medical Sciences, University of Phayao. Krissana Khoothiam Division of Microbiology, School of Medical Sciences, University of Phayao, Phayao, Thailand Orada Chumphukam Division of Biochemistry, Faculty of Medical Sciences, University of Phayao. Rungthip Thongboontho Division of Biochemistry, Faculty of Medical Sciences, University of Phayao. Panida Oonlao Division of Biochemistry, Faculty of Medical Sciences, University of Phayao. Piyawan Nuntaboon Division of Biochemistry, Faculty of Medical Sciences, University of Phayao. Kanokkarn Phromnoi Division of Biochemistry, Faculty of Medical Sciences, University of Phayao. https://orcid.org/0000-0002-8003-6112 DOI: https://doi.org/10.31989/ffhd.v13i2.1070 Abstract Background: Aging is a process caused by oxidants and aging-related enzymes. Therefore, the inhibition of these processes can exacerbate anti-aging agents. This study aimed to evaluate the antioxidant and anti-aging activities of leaf extracts from seven herbs used in traditional Thai herbal remedies.  Methods: Researchers assessed the total levels of phenolic content (TPC), antioxidant, anti-collagenase, and anti-tyrosinase activity using colorimetric methods. Cytotoxicity effects were determined using MTT assays, and matrix metalloproteinases(MMPs) secretion was assessed through gelatin zymography. In addition, inhibitory effects on the growth of microorganisms were examined using the disc diffusion and broth microdilution method. Results: TPC ranged between 48.68–440.91 mg GAE/g of ethanolic leaf extracts. High antioxidant activities against ABTS radicals were detected in P. granatum, P. emblica, P. guajava, T. bellirica, and T. chebula, while high DPPH neutralization appeared in M. coreia, P. guajava, and P. granatum. FRAP assays significantly reduced the power of T. chebula and P. granatum. T. chebula and P. guajava exhibited the highest inhibitory effect on H2O2induced ROS production. P. emblica, P. guajava, T. bellirica, and E. hygrophilus reduced tyrosinase and collagenase activities.  P. gaujava, T. chebula, and T. bellirica were shown to inhibit the secretion of MMP-2 from fibroblast cells. All concentrations of leaf extracts were non-toxic to fibroblast cells. P.granatum and T. bellirica could inhibit the growth of P. acnes, E. coli, and S. aureus.  Conclusion: Preliminary studies showed that P. granatum and T. bellirica leaf extracts have antioxidants, anti-aging, and anti-bacterial activitiesand have potential for use as active ingredients for the development of functional foods and cosmetic products. Keywords: antioxidants, anti-aging, leaf extracts Author Biographies Krissana Khoothiam, Division of Microbiology, School of Medical Sciences, University of Phayao, Phayao, Thailand - Orada Chumphukam, Division of Biochemistry, Faculty of Medical Sciences, University of Phayao. - Kanokkarn Phromnoi, Division of Biochemistry, Faculty of Medical Sciences, University of Phayao. - Downloads [Abstract] [Full Article] Published 2023-02-27 Issue Vol. 13 No. 2 (2023): February 2023 Section Research Articles License Authors retain the copyright of their articles and grant the Functional Food Center (FFC) and its journals the right of first publication under the terms of the Creative Commons Attribution 4.0 International License. This license permits unrestricted use, distribution, and reproduction in any medium, including commercial use, provided the original author(s) and source are properly credited. 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