Evaluation of antioxidant potential, DNA damage and hepatoprotective properties of Lagenaria siceraria plant against acetaminophen induced hepatotoxicity | Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007 Skip to main content Skip to main navigation menu Skip to site footer Open Menu Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007 Home Food Science Publisher Functional Food Center Current Archives About About the Journal Aims and Scope Contact Editing Services Editorial Team Express Peer Review Graphical Abstract Journal Insights Privacy Statement Submissions Announcements Search Search Register Login Home / Archives / Vol. 13 No. 3 (2023): March 2023 / Research Articles Evaluation of antioxidant potential, DNA damage and hepatoprotective properties of Lagenaria siceraria plant against acetaminophen induced hepatotoxicity Authors Upma Singh Pankaj Singh Pradeep Kumar Singh Shikha Shukla Ram Lakhan Singh Sukanta Mondal DOI: https://doi.org/10.31989/ffhd.v13i3.1072 Abstract Introduction: Lagenaria siceraria has various pharmacological effects like antioxidant, anti-hyperglycemic, anti-ulcer, anti-cancer, hepatoprotective, antimicrobial, anti-hyperlipidemic, anti-inflammatory and cardioprotective activities and is used to treat various diseases like diabetes, jaundice, piles, ulcers, hypertension, colitis, insanity, and skin diseases. Context and purpose of the study: The aim of the present study was to evaluate the phytochemical content, antioxidant activity, DNA damage and hepatoprotective activities of Lagenaria siceraria (LS)plant’s parts to explore their nutraceutical value. Methods: Phytochemical content was measured by the estimation of total phenolics, carotenoids, ascorbic acid, protein and carbohydrates while antioxidant activities were investigated by assaying the parameters of free radical scavenging activity, lipid peroxidation, superoxide anion radical scavenging activity, reducing power, and DNA damage protection assay. Hepatoprotective activity of LS leaf was measured by assaying cell viability, superoxide dismutase activity, malondialdehyde and nitric oxide formation. Results: Among the tested plant samples, LS leaves showed high contents of total phenolics, carotenoids, ascorbic acid, and protein contents whereas LS fruits have high carbohydrate contents. Evaluation of antioxidant activities in different parts of the LS plant showed that the LS leaf has high antioxidant properties as compared to other plant parts. The L. siceraria leaf also showed DNA protection activities against Fenton’s reagent as well as UV induced hydroxyl radical’s damage. To test the hepatoprotective activity of LS leaf against acetaminophen, induced hepatocellular toxicity and leaf extract concentrations of 2.5, 5.0 and 7.5 μg/104 cells were administered to cultured hepatocytes before acetaminophen exposure. The experimental results showed that LS leaf extract restores superoxide dismutase activity and reduces malondialdehyde and nitric oxide formation as compared to acetaminophen alone treated cultured cells. Conclusion: Our findings suggest that leaves of L. siceraria plants have high antioxidant potential and DNA damage-protective and hepatoprotective activities. Hence, the leaf part of this plant may be used in different food applications to provide nutritional and health benefits, as well as in various liver care herbal formulations. Keywords: Antioxidant, Hepatoprotective activity, Hepatocytes, Lipid peroxidation, Total phenolics  Downloads [Abstract] [Full Article] Published 2023-03-16 Issue Vol. 13 No. 3 (2023): March 2023 Section Research Articles License Authors retain the copyright of their articles and grant the Functional Food Center (FFC) and its journals the right of first publication under the terms of the Creative Commons Attribution 4.0 International License. This license permits unrestricted use, distribution, and reproduction in any medium, including commercial use, provided the original author(s) and source are properly credited. Authors may post and share their published work freely, provided that the original publication in this journal is acknowledged. By submitting to this journal, authors confirm that their manuscripts are original, not under consideration elsewhere, and that they hold the necessary rights to grant this license. The Functional Food Center encourages open scientific exchange and allows derivative and extended works, provided attribution to the original publication is maintained. About the Journal      Editing Services      Graphical Abstract      Journal Insights        Submissions        Editorial Team       Privacy Statement        Contact        Current        Archives   Open Journal Systems Hosting and Support by: OpenJournalSystems.com