Helichrysum arenarium as a source of flavonoids: Evaluation of antimicrobial activity and flavonoid content of extracts of Helichrysum flowers in vitro | Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007 Skip to main content Skip to main navigation menu Skip to site footer Open Menu Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007 Home Food Science Publisher Functional Food Center Current Archives About About the Journal Aims and Scope Contact Editing Services Editorial Team Express Peer Review Graphical Abstract Journal Insights Privacy Statement Submissions Announcements Search Search Register Login Home / Archives / Vol. 14 No. 1 (2024): January 2024 / Research Articles Helichrysum arenarium as a source of flavonoids: Evaluation of antimicrobial activity and flavonoid content of extracts of Helichrysum flowers in vitro Authors Asya Badalyan Syuzanna Abrahamyan Arevik Abovyan Armen Badalyan Gayane Semerjyan Narine Hovhannisyan DOI: https://doi.org/10.31989/ffhd.v14i1.1257 Abstract Background: Helichrysum (family Asteraceae, kind of H. armenium, subfamily of Asteroideae) is known in Armenian traditional medicine for its diuretic, antioxidant, choleretic and anti-inflammatory properties. Studies have shown that Helichrysum flowers have a high content of flavonoids. In this study, we investigated the impact of various extraction process parameters on the yield of flavonoids obtained from Helichrysum flowers in Armenia. Subsequently, a comprehensive antimicrobial assay was conducted on the ethanol and aqueous extracts derived from the dried flower heads of Helichrysum.  Objective: The objective of this study is to explore the influence of extraction process parameters on the yield of flavonoids from H. arenarium flowers and to determine their antibacterial activity. Methods: Antibacterial activity was determined using the agar-well diffusion method against four bacterial species (E. coli, Salmonella enterica, Staphylococcus aureus and S. typhimirium). The assessment of the total flavonoid content in the ethanol extract of Helichrysum was conducted using the AlCl3 method, following the procedure outlined by Andreeva and Kalinkina.  Results: The ethanolic and aqueous extracts demonstrated antibacterial activity against Staphylococcus aureus ATCC 6538 and Bacillus Subtilis 168. However, no significant activity was observed against Escherichia coli ATCC 8739 and Salmonella Typhimurium ATCC 14028. The research findings affirm that the extract derived from everlasting flowers exhibits antimicrobial properties, particularly targeting Gram-positive bacteria.  Conclusion: The results of our tests indicate that the extract of Helichrysum belonging to Armenian flora, is rich in flavonoids and has significant antimicrobial activity and may find application in the food industry, as well as in organic agriculture. Keywords: Helichrysum, plant extracts, flavonoids, antimicrobial activity, spectrophotometry Downloads [Abstract] [Full Article] Published 2024-01-09 Issue Vol. 14 No. 1 (2024): January 2024 Section Research Articles License Authors retain the copyright of their articles and grant the Functional Food Center (FFC) and its journals the right of first publication under the terms of the Creative Commons Attribution 4.0 International License. This license permits unrestricted use, distribution, and reproduction in any medium, including commercial use, provided the original author(s) and source are properly credited. Authors may post and share their published work freely, provided that the original publication in this journal is acknowledged. By submitting to this journal, authors confirm that their manuscripts are original, not under consideration elsewhere, and that they hold the necessary rights to grant this license. The Functional Food Center encourages open scientific exchange and allows derivative and extended works, provided attribution to the original publication is maintained. About the Journal      Editing Services      Graphical Abstract      Journal Insights        Submissions        Editorial Team       Privacy Statement        Contact        Current        Archives   Open Journal Systems Hosting and Support by: OpenJournalSystems.com