Protein hydrolysates and recovery of muscle damage following eccentric exercise | Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007 Skip to main content Skip to main navigation menu Skip to site footer Open Menu Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007 Home Food Science Publisher Functional Food Center Current Archives About About the Journal Aims and Scope Contact Editing Services Editorial Team Express Peer Review Graphical Abstract Journal Insights Privacy Statement Submissions Announcements Search Search Register Login Home / Archives / Vol. 5 No. 1 (2015): January 2015 / Research Articles Protein hydrolysates and recovery of muscle damage following eccentric exercise Authors Dale M.J Alliance for Research in Exercise, Nutrition and Activity (ARENA), Sansom Institute for Health Research and School of Health Sciences, University of South Australia, Adelaide, Australia; Thomson R.L Alliance for Research in Exercise, Nutrition and Activity (ARENA), Sansom Institute for Health Research and School of Health Sciences, University of South Australia, Adelaide, Australia; Coates A.M Alliance for Research in Exercise, Nutrition and Activity (ARENA), Sansom Institute for Health Research and School of Health Sciences, University of South Australia, Adelaide, Australia; Howe P.R.C Alliance for Research in Exercise, Nutrition and Activity (ARENA), Sansom Institute for Health Research and School of Health Sciences, University of South Australia, Adelaide, Australia; Clinical Nutrition Research Centre, University of Newcastle, Newcastle, Australia; Brown A Murray Goulburn Co-operative Co. Ltd, Melbourne, Australia Buckley J.D Alliance for Research in Exercise, Nutrition and Activity (ARENA), Sansom Institute for Health Research and School of Health Sciences, University of South Australia, Adelaide, Australia; DOI: https://doi.org/10.31989/ffhd.v5i1.164 Abstract Background: A whey protein hydrolysate (NatraBoost XR; WPHNB) has been shown to speed repair muscle damage. We sought to determine whether this benefit is specific to this hydrolysate to evaluate a marker for quality control. Methods: Three hydrolysates of the same whey protein isolate (WPI) were prepared (WPHNB, WPH1 and WPH2). Isometric knee extensor strength was measured in 39 sedentary male participants before and after 100 maximal eccentric contractions of the knee extensors to induce muscle damage. Participants were then randomised to consume 250 ml of flavoured water (FW, n=9), or 250 ml of FW containing 25 g of either NatraBoost XR (n=3), WPH1 (n=9), WPH2 (n=9) or WPI (n=9). Strength was reassessed over the next seven days while the supplements were consumed daily. Fibroblasts were cultured for 48 hr in the presence of the different hydrolysates, WPI, saline or fetal bovine serum to ascertain effects on cell proliferation. Results: Strength was reduced in all treatment groups after eccentric exercise (P<0.001). Strength recovered steadily over 7 days in the FW, WPI, WPH1 and WPH2 treatment groups (P<0.001), with no difference between treatments (P=0.87). WPHNB promoted faster strength recovery compared with the other treatments (P<0.001). Fibroblast proliferation was greater with WPHNB compared with saline, WPI or the other hydrolysates (P<0.001). Conclusions: Promoting recovery from muscle damage seems unique to WPHNB. In vitro fibroblast proliferation may be a useful marker for quality control. It is not clear whether effects on fibroblast proliferation contribute to the in vivo effect of WPHNB on muscle damage. Keywords: tissue repair, fibroblast proliferation, strength recovery, whey protein Downloads [Abstract] [Full Article] Published 2015-01-31 Issue Vol. 5 No. 1 (2015): January 2015 Section Research Articles License Authors retain the copyright of their articles and grant the Functional Food Center (FFC) and its journals the right of first publication under the terms of the Creative Commons Attribution 4.0 International License. This license permits unrestricted use, distribution, and reproduction in any medium, including commercial use, provided the original author(s) and source are properly credited. Authors may post and share their published work freely, provided that the original publication in this journal is acknowledged. By submitting to this journal, authors confirm that their manuscripts are original, not under consideration elsewhere, and that they hold the necessary rights to grant this license. The Functional Food Center encourages open scientific exchange and allows derivative and extended works, provided attribution to the original publication is maintained. About the Journal      Editing Services      Graphical Abstract      Journal Insights        Submissions        Editorial Team       Privacy Statement        Contact        Current        Archives   Open Journal Systems Hosting and Support by: OpenJournalSystems.com