9-cis β-carotene Inhibits Atherosclerosis Development in Female LDLR-/- Mice | Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007 Skip to main content Skip to main navigation menu Skip to site footer Open Menu Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007 Home Food Science Publisher Functional Food Center Current Archives About About the Journal Aims and Scope Contact Editing Services Editorial Team Express Peer Review Graphical Abstract Journal Insights Privacy Statement Submissions Announcements Search Search Register Login Home / Archives / Vol. 5 No. 2 (2015): February 2015 / Research Articles 9-cis β-carotene Inhibits Atherosclerosis Development in Female LDLR-/- Mice Authors Noa Zolberg Relevy The Bert W. Strassburger Lipid Center; Sheba Medical Center, Tel-Hashomer, 5265601 Israel; The Mina and Everard Goodman Faculty of Life Science, Bar-Ilan University, Ramat-Gan, Israel Ralph Rühl Department of Biochemistry and Molecular Biology, University of Debrecen, Debrecen, Hungary Ayelet Harari The Bert W. Strassburger Lipid Center; Sheba Medical Center, Tel-Hashomer, 5265601 Israel Itamar Grosskopf The Bert W. Strassburger Lipid Center; Sheba Medical Center, Tel-Hashomer, 5265601 Israel Iris Barshack Institute of Pathology, Sheba Medical Center, Tel-Hashomer; Sackler Faculty of Medicine, Tel-Aviv University, Israel Ami Ben-Amotz N.B.T Israel Uri Nir The Mina and Everard Goodman Faculty of Life Science, Bar-Ilan University, Ramat-Gan, Israel Hugo Gottlieb Department of Chemistry, Bar-Ilan University, Ramat-Gan 52900, Israel. Yehuda Kamari The Bert W. Strassburger Lipid Center; Sheba Medical Center, Tel-Hashomer, 5265601 Israel; Sackler Faculty of Medicine, Tel-Aviv University, Israel; Dror Harats The Bert W. Strassburger Lipid Center; Sheba Medical Center, Tel-Hashomer, 5265601 Israel; Sackler Faculty of Medicine, Tel-Aviv University, Israel; Aviv Shaish The Bert W. Strassburger Lipid Center; Sheba Medical Center, Tel-Hashomer, 5265601 Israel DOI: https://doi.org/10.31989/ffhd.v5i2.172 Abstract Background: Several epidemiological studies have shown that diets rich in carotenoids are associated with a reduced risk of cardiovascular disease. However, administration of synthetic all-trans b-carotene was reported to have no effect on cardiovascular disease. We previously showed that the 9-cis b-carotene-rich powder of the alga Dunaliella bardawil inhibits atherogenesis and reduces plasma non-HDL cholesterol levels in mice. Context and purpose of this study: We sought to study whether isolated 9-cis b-carotene inhibits atherogenesis in a murine model of atherosclerosis. Results: Twelve-week-old female LDL receptor knockout mice (LDLR-/-) were pretreated for  2 weeks with regular chow diet fortified with the alga Dunaliella powder, 9-cis β-carotene isomer, all-trans β-carotene isomer, or 9-cis retinoic acid, followed by 10 weeks of a high-fat diet with the same fortifications. In contrast to Dunaliella, 9-cis β-carotene did not inhibit the high fat diet-induced elevation of plasma cholesterol. In addition, diet fortification with Dunaliella powder, β-carotene isomers, or 9-cis retinoic acid did not change the plasma retinol or retinoic acid levels. Nevertheless, 9-cis β-carotene significantly inhibited atherogenesis compared to the control mice (39% reduction). Conclusions: The results suggest that 9-cis β-carotene should be considered as an anti-atherogenic agent in the human diet. Key words: Atherosclerosis, Dunaliella, 9CBC, LDLR-/- mice    Downloads [Abstract] [Full Article] Published 2015-02-15 Issue Vol. 5 No. 2 (2015): February 2015 Section Research Articles License Authors retain the copyright of their articles and grant the Functional Food Center (FFC) and its journals the right of first publication under the terms of the Creative Commons Attribution 4.0 International License. This license permits unrestricted use, distribution, and reproduction in any medium, including commercial use, provided the original author(s) and source are properly credited. Authors may post and share their published work freely, provided that the original publication in this journal is acknowledged. By submitting to this journal, authors confirm that their manuscripts are original, not under consideration elsewhere, and that they hold the necessary rights to grant this license. The Functional Food Center encourages open scientific exchange and allows derivative and extended works, provided attribution to the original publication is maintained. About the Journal      Editing Services      Graphical Abstract      Journal Insights        Submissions        Editorial Team       Privacy Statement        Contact        Current        Archives   Open Journal Systems Hosting and Support by: OpenJournalSystems.com