Processed coffee alleviates DSS-induced colitis in mice | Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007 Skip to main content Skip to main navigation menu Skip to site footer Open Menu Functional Foods in Health and Disease - Online ISSN: 2160-3855; Print ISSN: 2378-7007 Home Food Science Publisher Functional Food Center Current Archives About About the Journal Aims and Scope Contact Editing Services Editorial Team Express Peer Review Graphical Abstract Journal Insights Privacy Statement Submissions Announcements Search Search Register Login Home / Archives / Vol. 3 No. 5 (2013): May 2013 / Research Articles Processed coffee alleviates DSS-induced colitis in mice Authors Bernd L. Fiebich VivaCell Biotechnology GmbH, Ferdinand-Porsche-Str. 5, D-79211 Denzlingen Amaya G. Vinuesa Departamento de Biología Celular, Fisiología e Inmunología, Facultad de Medicina. Universidad de Córdoba, Avda Menendez Pidal s/n. 14004 Córdoba Gonzalo Sanchez-Duffhues Departamento de Biología Celular, Fisiología e Inmunología, Facultad de Medicina. Universidad de Córdoba, Avda Menendez Pidal s/n. 14004 Córdoba Juan A. Collado Departamento de Biología Celular, Fisiología e Inmunología, Facultad de Medicina. Universidad de Córdoba, Avda Menendez Pidal s/n. 14004 Córdoba Thorsten Rose VivaCell Biotechnology GmbH, Ferdinand-Porsche-Str. 5, D-79211 Denzlingen Jörn Menthe J.J. Darboven GmbH & Co KG, Pinkertweg 13, D- 22113 Hamburg Eduardo Muñoz Departamento de Biología Celular, Fisiología e Inmunología, Facultad de Medicina. Universidad de Córdoba, Avda Menendez Pidal s/n. 14004 Córdoba DOI: https://doi.org/10.31989/ffhd.v3i5.58 Abstract Background: Coffee is one of the most widely consumed beverages in the world and it has been demonstrated that it has important therapeutic activities not only because of its caffeine content but also owing to the presence of other biologically active small molecules such as chlorogenic acid, trigonelline and cyclopentadiones. However, chlorogenic acid is degraded into catechol, pyrogallol and hydroxyhydroquinone, which are thought to induce irritation of the gastric mucosa. To reduce the content of irritant compounds processing methods have been developed prior to roasting the coffee beans. Objectives: The aim of this study was to study the anti-inflammatory and gastro-protective effects of processed coffee (Idee-Kaffee) on in LPS-treated human primary monocytes and in a murine model of colon inflammation (IBD model). Results: In this study we have analyzed the effects on inflammatory events in cultured cells and in mice drinking a commercially available processed coffee. The processed coffee inhibited lipopolysaccharide (LPS)-induced proinflammatory cytokines such as interleukin (IL)-1β, tumor necrosis factor (TNF)α, IL-6 and IL-8, and other inflammatory mediators such as prostaglandin (PG)E2 and 8-isoprostane in cultured human primary monocytes. Oral administration of dissolved processed coffee, i.e., in its usual beverage form, improved greatly the adverse macroscopic and histological features of dextran sodium sulfate (DSS)-induced colitis in mice in a dose-dependent manner. Processed coffee not only largely prevented DSS-induced colitis but also dramatically suppressed in vivo NF-ϰB and STAT3 activities through inhibition of IϰBα and STAT3 phosphorylation. Furthermore, this soluble coffee bean extract reduced the expression of proinflammatory cytokines TNFα, IL-11, and IL-6 and the expression of cyclooxygenase (COX)-2 in colonic tissues. Conclusions: This work identified processed coffee as an anti-inflammatory beverage with the capacity to reduce substantially DSS-induced colitis as well as the colitis-associated cellular inflammatory events. Keywords: coffee, Inflammatory Bowel Disease, NF-ϰB, STAT3, cytokines Downloads [Abstract] [Full Article] Published 2013-05-30 Issue Vol. 3 No. 5 (2013): May 2013 Section Research Articles License Authors retain the copyright of their articles and grant the Functional Food Center (FFC) and its journals the right of first publication under the terms of the Creative Commons Attribution 4.0 International License. This license permits unrestricted use, distribution, and reproduction in any medium, including commercial use, provided the original author(s) and source are properly credited. 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