1 Supplemental Data for Koodalugodaarachchi, V., Kekulandara, D. S., Gimhani, D. R. (2022). Genetic diversity of Oryza sativa ‘Dahanala’ traditional red rice and molecular markers associated with trichome density on adaxial surfaces. Genetic Resources 3 (5), 10–23. doi: 10.46265/genresj.AVEO9374 Supplemental Data 1: Description of seed morphological characters – modified based on IPGRI rice descriptors (IPGRI, 2007) 1. Seed shape (7.5.22) Time: At maturity, shape of the grains is classified as: 1) Round 2) Semi-round 3) Half-spindle shaped 4) Spindle-shaped 5) Very spindle-shaped 2. Lemma and palea colour are classified into 19 classes (7.5.5) (modified): 0) Straw 1) Gold and/or gold furrows on straw background 2) Brown spots on straw 3) Brown furrows on straw 4) Brown 5) Reddish to light purple 6) Purple spots on straw background 7) Purple furrows on straw background 8) Purple 9) Black 10) White 11) Brownish black 12) Yellowish gray 13) Yellowish brown 14) Brown furrows on gold background 15) Reddish brown spots on gold background 16) Black spots on straw background 17) Brownish black furrows on gold background 18) Brownish black furrows on straw background 19) Brown furrows on yellowish background 3. Seed coat (bran) colour (7.5.23) (modified): Time: At maturity, brown rice (dehulled grains) is classified into: 0) Off white 1) White 2) Light Brown 3) Speckled brown 4) Brown 5) Red 6) Variable Purple 7) Purple 8) Off white with black apiculus 9) Off white with brown apiculus https://10.0.180.185/genresj.AVEO9374 2 10) Brown seed coat with white strips 11) Red seed coat with white strips 12) Off white seed coat with white strip with black apiculus 13) Off white seed coat with white strips 14) Brown seed coat with black apiculus 15) Light brown seed coat with white strip 16) Brown seed coat with white strip and black apiculus 4. Lemma and palea pubescence (7.5.4): Time: At maturity, pubescence of the hull is classified as: 1) Glabrous 2) Hairs on lemma keel 3) Hairs on upper portion 4) Short hairs 5) Long hairs (velvety) 5. Grain length (7. 5. 15): Time: At maturity, grain length is measured as the distance from the base of the lowermost sterile lemma to the tip (apiculus) of the fertile lemma or palea, whichever is longer. In the case of awned varieties, the grain is measured to a point comparable to the tip of the apiculus 6. Grain width (7. 5. 16): Time: At maturity, width of grain is measured in millimeters as the distance across the fertile lemma and the palea at the widest point 3 Supplemental Figure 1: Additional SSR marker profiles amplified from DNA bulks (RM201, RM220, RM241, RM259, RM270). The arrowhead indicates corresponding scored alleles. B1–B8, DNA bulks; B9, Bg360; L25, L100, L200, size markers; bp, basepairs. Supplemental Figure 2: PCR profile or heterogenic marker RM515 of DNA bulks. 4 Supplemental Figure 3: PCR profiles of expanded DNA bulks for SSR markers (RM84, RM207, RM224, RM480, RM518, RM536) Amplification of bulked DNA samples and expanded bulks with a) RM480 (Product size: 199–221bp), b) RM84 (Product size: 118–124bp), c) RM224 (Product size: 124–142bp), d) RM207 (Product size: 110–132bp), e) RM536 (Product size: 220–230bp), f) RM518 (Product size: 158–180bp) (resulted with monomorphic alleles) (Product size: 132– 154bp). The arrowhead indicates corresponding scored alleles. B1–B8, DNA bulks; B9, Bg360; L25, L100, L200, size markers; bp, basepairs. 8, 15, 3, 11, 6, 21, 7, 22, 28, 20, 32, 33, 19, 4, 5, 17, 31, 27, 23, 13, 16, 26, 30, 29, 9, 24, 12, 18, 14, 9, 24, 12, 18, 14 - Individual accessions included in bulks, numbered as in Table 1. b) 5 Supplemental Figure 4: PCR profiles of expanded DNA bulks for SSR markers (RM217, RM237, RM418, RM440, RM515, RM571) Amplification of bulked DNA samples and expanded bulks with a) RM237 (Product size: 124–138bp), b) RM571 (Product size: 182–192bp, c) RM418 (Product size: 245–290bp), d) RM440 (Product size: 161–217bp), e) RM515 (Product size: 211–219bp), f) RM217 (Product size: 114–144bp). The arrowhead indicates corresponding scored alleles. B1– B8, DNA bulks; B9, Bg360; L25, L100, L200, size markers; bp, basepairs. 8, 15, 3, 11, 6, 21, 7, 22, 28, 20, 32, 33, 19, 4, 5, 17, 31, 27, 23, 13, 16, 26, 30, 29, 9, 24, 12, 18, 14, 9, 24, 12, 18, 14 - Individual accessions included in bulks, numbered as in Table 1. 6 Supplemental Figure 5: Map position of markers RM277 and RM279 on rice chromosomes 12 and 2. (adapted from Temnykh et al., 2000) 7 References International Plant Genetic Resources Institute, and West Africa Rice Development Association. Descriptors for Wild and Cultivated Rice (Oryza Spp.). Biodiversity International, 2007. https://www.bioversityinternational.org/fileadmin/user_upload/online_library/ publications/pdfs/232.pdf Temnykh, S., Park, W.D., Ayres, N., Cartinhour, S., Hauck, N., Lipovich, L., Cho Y.G., Ishii, T., McCouch, S,R. (2000). Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.). Theoretical Applied Genetics 100, 697–712. https://www.bioversityinternational.org/fileadmin/user_upload/online_library/publications/pdfs/232.pdf https://www.bioversityinternational.org/fileadmin/user_upload/online_library/publications/pdfs/232.pdf