Georgian Scientists/ . 7 N 4, 2025 493 Georgian Scientists Vol. 7 Issue 4, 2025 https://doi.org/10.52340/gs.2025.07.04.33 1, 2, 3, 4, 5, 6 1 , .d.lagazidze@tsmu.edu 2 . 3 , . 4 , . 5 , . 6 , , . . ( , , W 35) - , . 1,0 - 1,25 / ( ). , , . : , , , , . , [1,2,3]. , , , . Georgian Scientists/ . 7 N 4, 2025 494 , , , , , . , , , [4]. . Thymus L. . ( ), . , , , [5]. , , , , . , - [2], , , [1,6] , [6,7,8]. , , . . . - - (Hippophae rhamnoides L.) ( ), (99%, CAS 89-83-8) Sigma-Aldrich. : , W35, : 400 1500, -80. . : , , . . Georgian Scientists/ . 7 N 4, 2025 495 . , , . . , , . : , W-35 . , [9]. , , . , , , [10]. . . , [11,12] ( . 1). 1. W35 - 38-400C . F1 F2 F3 , , , 0,5 0,5 0,5 0,1 0,1 0,1 -400:1500 (1:4) - - 2,4 W35 - 2,4 - 2,4 - - -80 - - 0,005 Georgian Scientists/ . 7 N 4, 2025 496 3 3 1-2 . . +(4-8)0C . (45±2°C ), 1500 - , (1:4) -400- , ( -80) . . [13,14,15,16]. - ( . 2). 2. - . -W35 (400:1500) , , , , % 1,0-1,25 1,0-1,25 1,0-1,25 , 2,6 – 2,7 2,9-3,1 2,9- 3,0 ,°C 32,7 35,4 35,6 , °C 25,7 26,4 25,6 9 9 10 , KOH 4,2 4,4 - , % I2 0,0075 0,0045 - , , 2,7 - 3,0 . , - Georgian Scientists/ . 7 N 4, 2025 497 , . -W35- , , , , . [14] 450 , Jasco-730 . . ( ) 1,0 – 1,25 . [15]. 1 1 Agilent Technologies 7890B , Agilent Technologies 5977A MSD. split/splitless . : HP-5ms Ultra Inhert (30m x 250µm x 0.25µm). . 2500C, . 2800C . 900C 2 , 900C 2800C (20C/ ); 2800C 10 . 2800C ( . 1). .1. . NIST- [16]. -3 . 3. . , % Georgian Scientists/ . 7 N 4, 2025 498 (C14:0) 0,31 – 0,35 (C16:0) 1,89 – 1,95 (C16:0) 28,63 – 28,67 (C17:0) 0,29 – 0,33 (C18:0) 1,96 – 0,99 (C18:0) 31,41 – 31,45 (C18:0) 27,30 – 27,34 (C 20:0) 1,42 – 1,44 , , . ( ) W 35 - . 2 . In vitro BJ- II (Disintegration tester) . 400 30% , -(30 ). 37°±2°C. : 30 . 5-5 30% . . ( .2). .2. . -10 0 10 20 30 40 50 60 0 50 100 150 200 250 300 C, % T, min Georgian Scientists/ . 7 N 4, 2025 499 , W 35 - 53 - 55 %- . USP (Dissolution Tester DT 126/128 light). 400 30% ). 37°±2°C. . . : .3. , W 35 - 5-10 30% , 60-65%. . , - , W35- . , . , 1,0-1,25 ( ). In vitro W 35- . , 30% 55-65%. 0 10 20 30 40 50 60 70 0 5 10 15 20 25 30 35 C, % T, min ( ) Georgian Scientists/ . 7 N 4, 2025 500 1. ., . . , . 2010. . 8, N 6. . 9-12. 2. . . . 2016. N 2(15). . 60-69. 3. . . 2010 2, c.31-33. 4. Lagazidze D., Kutateladze G., Orjonikidze M., Getia M., Bakuridze A., Development and standardization of Sea-buckthorn (Hippophae ramnoides) suppositories, Georgian Scientists, Vol.5 Issue 2, 2023, https://doi.org/10.52340/gs.2023.05.02.34. 5. . (THYMOL), https://www.vidal.ru/drugs/molecule-in/1042. 6. . . , , , , 2004, #2, c-249-253. 7. . . 1997,#12, 90-93. 8. . c : , , 2016 N4(17), c.92-97. 9. USP Pharmacists,Pharmacopeia,The United State Pharmacopeial,Inc.,Rockville(2015). 10. European Pharmacopoeia,4th ed.,Strasbourg, Counsil of Europe (2015). 11. ., , , 2006. 12. ., 2, , 2003, . 80-82. 13. 1.4.1.0013.15. 14. -42-1730-98 . 15. -42-0057 407 203 0,1 . 16. Kovats,(1958) Characterization of organic compounds by gas chromatographi.Part 1.retention indices of aliphatic halides, alcohols, aldehydes and ketones. Helvetica Chimica Acta,41,1915-1932. DEVELOPMENT OF SUPPOSITORIES CONTAINING SEA BUCKTHORN OIL AND THYMOL Lagazidze Dimitri1, Benashvili Tamuna2, Orjonikidze Manana 3, Kikalishvili Bela 4, Getia Malkhaz5, Bakuridze Alyosha6. 1Doctor of Pharmacy, Senior Researcher at the Iovel Kutateladze Institute of Pharmacochemistry, TSMU; 2Master student at the Department of Pharmaceutical Technology, TSMU; 3Doctor of Pharmacy, Senior Researcher at the Iovel Kutateladze Institute of Pharmacochemistry, TSMU; 4Doctor of Pharmacy, Head Researcher at the Iovel Kutateladze Institute of Pharmacochemistry, TSMU; 5Doctor of Pharmacy, Head Researcher at the Iovel Kutateladze Institute of Pharmacochemistry, TSMU; 6Doctor of Pharmaceutical Sciences, Professor, Tbilisi State Medical University, Head of the Department of Pharmaceutical Technology. ABSTRACT Georgian Scientists/ . 7 N 4, 2025 501 The study is devoted to the development and quality assessment of combined vaginal suppositories containing sea buckthorn oil and thymol with anti-inflammatory and antiseptic effect. The physical, chemical and technological properties of model samples prepared using three different bases (cocoa butter, polyethylene glycol of different molecular weights, Vitepsol W 35) have been studied, the suppository base has been selected on the basis of biopharmaceutical studies and the formulation has been determined. The total content of carotenoids is 1.0 - 1.25 mg / suppository (calculated on -carotene). The identification of fatty acids has been carried out, among which triglycerides of oleic, palmitic and stearic acids are dominant.