Georgian Scientists/ . 7 N 4, 2025 557 Georgian Scientists Vol. 7 Issue 4, 2025 https://doi.org/10.52340/gs.2025.07.04.39 1; 2 3; 4 1 ; 2 ; 3 ; ; 4 ; ; OARSI- Grade 4, Grade 5 Grade 6 . 20 . - 2019-2025 . , AgNOR (CD45) (Ki67). , . AgNOR , Ki67- . : , , CD45, AgNOR, OARSI; Georgian Scientists/ . 7 N 4, 2025 558 , , . 2019 528 . 113%- 1990 1. , , . , , , , 2. . , . 50 , 50 . , 18,5%- 28,6%- . , , . . , , 3. , , . , , 4. OARSI- (osteoarthritis cartilage histopathology assessment system) . , 5. OARSI- . 6. Georgian Scientists/ . 7 N 4, 2025 559 , ; . , . , . , . . . Grade 0 - . . , . . Grade 1 - . , . ”, . Grade 2 - Grade 3 - . Grade 4 - . ; , . . Grade 5 - . , , - . , . Grade 6 - , ). . , , ; , . Georgian Scientists/ . 7 N 4, 2025 560 , . , 7. , . , ) . , , , ( ) . , 8 9 . . ( ) . , . , , . . , , 80%- Ki67- , , 10. , . (thymidin labeling index), (flow cytometry) Ki-67 AgNOR .11–13 AgNOR- , , . 14–16. , Georgian Scientists/ . 7 N 4, 2025 561 , . AgNOR . AgNOR . AgNOR- 17–19. . - 2019-2025 . 60 , . : Grade 4 - 20 Grade 5 - 20 Grade 6 - 20 , AgNOR (CD45) (Ki67). OARSI (The OARSI osteoarthritis cartilage histopathology assessment system). , CD45- . Georgian Scientists/ . 7 N 4, 2025 562 : OARSI Grade 4 30%- ; OARSI Grade 5 45%- ; OARSI Grade 6 55%- ; 1: - ; 1: CD45 - . IHC, CD45, 100x; Georgian Scientists/ . 7 N 4, 2025 563 OARSI Grade 4 27%- , OARSI Grade 5 49%- , OARSI Grade 6 58%- ; , AgNOR , Ki67- 2: ; 2: CD45 . IHC, CD45, 200x; Georgian Scientists/ . 7 N 4, 2025 564 , , AgNOR , OARSI Grade 4 12%- , OARSI Grade 5 14%- , OARSI Grade 6 21%- ; Ki67- , , OARSI Grade 4 21%- , OARSI Grade 5 26%- , OARSI Grade 6 30%- ; 3: - ; Georgian Scientists/ . 7 N 4, 2025 565 3: - , AgNOR, 200x AgNOR OARSI Grade 4 16%- ; OARSI Grade 5 - 17%- ; OARSI Grade 6 - 24%- ; Ki67- OARSI Grade 4 27%- ; Grade 5 - 29%- , Grade 6 - 32%- ; 4: ; Georgian Scientists/ . 7 N 4, 2025 566 4: , AgNOR, 200x; , „- “- , 20. , , , , , , . , , . , , , . , . AgNOR , Ki67- . Georgian Scientists/ . 7 N 4, 2025 567 1. Abbafati, C. et al. Global burden of 369 diseases and injuries in 204 countries and territories, 1990-2019: a systematic analysis for the Global Burden of Disease Study 2019. Lancet 396, 1204–1222 (2020). 2. Lee, K. M. et al. Risk Factors for Osteoarthritis and Contributing Factors to Current Arthritic Pain in South Korean Older Adults. Yonsei Med J 56, 124–131 (2014). 3. Knights, A. J., Redding, S. J. & Maerz, T. Inflammation in osteoarthritis: the latest progress and ongoing challenges. Curr Opin Rheumatol 35, 128–134 (2023). 4. Knights, A. J., Redding, S. J. & Maerz, T. Inflammation in osteoarthritis: the latest progress and ongoing challenges. Curr Opin Rheumatol 35, 128–134 (2023). 5. Pritzker, K. P. H. et al. Osteoarthritis cartilage histopathology: grading and staging. Osteoarthritis Cartilage 14, 13–29 (2006). 6. Pritzker, K. P. H. et al. Osteoarthritis cartilage histopathology: Grading and staging. Osteoarthritis Cartilage 14, 13–29 (2006). 7. Hall, A. C. The Role of Chondrocyte Morphology and Volume in Controlling Phenotype-Implications for Osteoarthritis, Cartilage Repair, and Cartilage Engineering. Curr Rheumatol Rep 21, (2019). 8. Boyde, A. The Bone Cartilage Interface and Osteoarthritis. Calcif Tissue Int 109, 303–328 (2021). 9. Mathiessen, A. & Conaghan, P. G. Synovitis in osteoarthritis: current understanding with therapeutic implications. Arthritis Res Ther 19, (2017). 10. Hoshiyama, Y. et al. Chondrocyte clusters adjacent to sites of cartilage degeneration have characteristics of progenitor cells. Journal of Orthopaedic Research 33, 548–555 (2015). 11. Adamashvili, N., Beriashvili, R., Tevzadze, N., Kepuladze, S. & Burkadze, G. Features of the distribution of acute and chronic inflammatory index in cervical intraepithelial neoplasia of different degrees and its relationship with proliferative activity detected by AgNOR technology. GEORGIAN SCIENTISTS https://doi.org/10.52340/gs.2024.06.01.08 (2024) doi:10.52340/gs.2024.06.01.08. 12. Meshveliani, P., Didava, G., Tomadze, G., Kepuladze, S. & Burkadze, G. Evaluation of proliferative activity of pre-tumor and tumor processes of Barrett’s Georgian Scientists/ . 7 N 4, 2025 568 esophagus using AGNOR technology. GEORGIAN SCIENTISTS https://doi.org/10.52340/gs.2023.05.02.07 (2023) doi:10.52340/gs.2023.05.02.07. 13. Tavdgiridze, N., Tevdorashvili, G., Kepuladze, S. & Burkadze, G. Assessment of proliferative activity of immature ovarian teratomas using AgNOR technology. GEORGIAN SCIENTISTS https://doi.org/10.52340/gs.2023.05.01.20 (2023) doi:10.52340/gs.2023.05.01.20. 14. Kveliashvili, T., Didava, G., Kepuladze, S. & Burkadze, G. Peculiarities of the proliferative activity of the gallbladder mucosa in precancerous and cancerous pathologies detected by AgNOR technology. GEORGIAN SCIENTISTS https://doi.org/10.52340/gs.2023.05.04.31 (2023) doi:10.52340/gs.2023.05.04.31. 15. Turashvili, T., Tevdorashvili, G., Burkadze, G. & Kepuladze, S. Evaluation of proliferative activity of endometrial metaplasias by AgNor technology. GEORGIAN SCIENTISTS https://doi.org/10.52340/2023.05.03.02 (2023) doi:10.52340/2023.05.03.02. 16. Metreveli, B., Gagua, D., Burkadze, G. & Kepuladze, S. Proliferative characteristics of eutopic and ectopic endometrium in adenomyosis using AgNOR technology. GEORGIAN SCIENTISTS https://doi.org/10.52340/gs.2023.05.01.04 (2023) doi:10.52340/gs.2023.05.01.04. 17. Svanadze, T., Kepuladze, S., Tevzadze, N. & Burkadze, G. ASSESSMENT OF PROLIFERATIVE ACTIVITY OF DIFFERENT TYPES OF SQUAMOUS CELL METAPLASIA OF THE CERVIX USING AgNor TECHNOLOGY. GEORGIAN SCIENTISTS https://doi.org/10.52340/gs.2023.05.02.35 (2023) doi:10.52340/gs.2023.05.02.35. 18. Arveladze, G., Beriashvili, R., Kepuladze, S. & Burkadze, G. Evaluation of proliferative activity of different subtypes of basal cell carcinomas by AgNOR technology. GEORGIAN SCIENTISTS https://doi.org/10.52340/gs.2023.05.04.19 (2023) doi:10.52340/gs.2023.05.04.19. 19. Barbakadze, L., Kintraia, N., Burkadze, G. & Kepuladze, S. ASSESSMENT OF PROLIFERATIVE ACTIVITY OF ENDOMETRIAL STROMAL TUMORS USING AgNOR TECHNOLOGY. GEORGIAN SCIENTISTS https://doi.org/10.52340/gs.2023.05.02.16 (2023) doi:10.52340/gs.2023.05.02.16. 20. Berenbaum, F. Osteoarthritis as an inflammatory disease (osteoarthritis is not osteoarthrosis!). Osteoarthritis Cartilage 21, 16–21 (2013). Georgian Scientists/ . 7 N 4, 2025 569 Peculiarities of changes in the proliferative activity and inflammatory index of the osteochondral component and synovial membrane of the hip joint during coxarthrosis Nino Kavtaradze1; Elene-Dodo Kartvelishvili2; Magda Jangiani3; Giorgi Burkadze4 1PhD student at Tbilisi State Medical University; 2Emeritus Professor at Tbilisi State Medical University; 3Resident of the Department of Pathological Anatomy of Tbilisi State Medical University; Doctor; 4Head of the Department of Molecular Pathology of Tbilisi State Medical University; Professor; Doctor of Pathology Abstract The aim of the study was a comparative analysis of inflammatory changes and proliferative activity in the osteochondral component and synovial membranes of the joint according to the OARSI classification in Grade 4, Grade 5 and Grade 6 groups. Each study group included 20 cases. The study material used for the study consisted of depersonalized paraffin blocks from the archive material of the TSMU Educational-Scientific and Diagnostic Laboratory for the years 2019-2025. The study used hematoxylin & eosin technology, AgNOR technology to assess proliferative activity, and immunohistochemical technology to assess inflammatory index (CD45) and proliferative activity (Ki67). As the analysis of the results showed, in osteoarthritis, the inflammatory index increases with the degree of damage and it is manifested in almost the same way in the bone-cartilage component and synovial membranes. The dynamics of proliferative activity is manifested in the same way both with AgNOR technology and with the use of the proliferative marker Ki67 and it increases in accordance with the degree of damage. Keywords: Synovial membrane, proliferation, CD45, AgNOR, OARSI; - ; Nino Kavtaradze - PhD student at Tbilisi State Medical University; n.qavtaradze@yahoo.com - ; Elene-Dodo Kartvelishvili - Emeritus Professor at Tbilisi State Medical University; n.qavtaradze@yahoo.com Georgian Scientists/ . 7 N 4, 2025 570 - ; ; Magda Jangiani - Resident of the Department of Pathological Anatomy of Tbilisi State Medical University; Doctor; jangianimagda@gmail.com - , ; George Burkadze - Professor at Tbilisi State Medical University, Head of the Department of Molecular Pathology; E-mail: burkadze@yahoo.com g.burkadze@tsmu.edu Phone Number: 599578833 ORCID: https://orcid.org/0000-0002-5028-4537