Georgian Scientists/ . 7 N 4, 2025 657 Georgian Scientists Vol. 7 Issue 4, 2025 https://doi.org/10.52340/gs.2025.07.04.45 (Cotinus Coggygria Scop) ( ) 1, 1, 3, 1, 3, 2, 2, 2, 2 1 , ; 2 . ; 3 : . . . , , . , , , , , ; ), in vivo . : , , , , , , , Enterecoccus faecalis. Georgian Scientists/ . 7 N 4, 2025 658 , . . , . , , . (NaOCl), , (EDTA) [1] , , (NaOCl) Enterecoccus faecalis [2]. . , , , , . . , , [3, 4]. , 700 . , , [5]. , . , , , . Georgian Scientists/ . 7 N 4, 2025 659 , , , [6]. , . . . , . . , , , , , , [7, 8]. (Cotinus Coggygria Scop) ) ( ) , , , NaOCl [9]. , , , , , , , , . , . , , (Cotinus Coggygria Scop) , , , , , . [10, 11, 12]. (Cotinus Coggygria Scop) (Anacardiaceae Lind) . : Cotinus Mill - , Postecia L - Rhus L. Georgian Scientists/ . 7 N 4, 2025 660 : Cotinus Coggygria Scop Cotinus Americanus Hutt, [13]. , , . . , ( ) ( ) . , 32 % [14, 15]. ( ) ( ) 1:5, ¼ . , , . , , ( . 1) Georgian Scientists/ . 7 N 4, 2025 661 1. (Cotinus Coggygria Scop.) HPLC/UV HPLC/MS 22 . : , , , , 3-0- -D , 3-0-a-L , , . , ( ) [16, 17]. , ) Agilent Tecnlolgies 1260. Eclipce plus C 18 (250mmx4,6mmx5µm); : ; 1 . UV 270 nm. ( ) ( ) : ; , , , 3-0- -D . ( 2) [16, 17, 18]. Georgian Scientists/ . 7 N 4, 2025 662 2.1 2.2 2. 2.1. ) . 2.2 ( ) . ( ) . (2450 MHz, 650 W) 7-10 . Giuliani- , Behera- Jeevitha- [1-3] (ICMSF) . , 3) [19, 20, 21]. 3.1 3.2 Georgian Scientists/ . 7 N 4, 2025 663 3.3 3.4 3. 3.1 ( ) . 3.2 ( ) . 3.3 ( ) . 3.4 ( ) . , , , ( ) . ( ) , , . . , (CLCT) ) (CLTF). Georgian Scientists/ . 7 N 4, 2025 664 , . (CLTF), (CLCT) . , (CLTF) , (CLCT), , ; CLCT CLTF- , . CLCT CLTF , [22, 23, 24]. . In vitro ,, 90’’. . - Enterococcus faecalis, Streptococcus ssp. 2% 5% 10% 15%. , 2% 5% , 10 % 15 % Enterococcus faecalis Streptococcus ssp . , , , ( ) . [25] - Georgian Scientists/ . 7 N 4, 2025 665 . : 10% 15% , , . , , . in vivo . - . 1. Brenda P F A Gomes, Emelly Aveiro, Anil Kishen. Irrigants and irrigation activation systems in Endodontics. Pub med, Braz Dent J. 2023 Jul-Aug;34(4):1-33. doi: 10.1590/0103- 6440202305577. 2. Kenan Tunç*, Ay egül Ho , Bilgen Güne Investigation of Antibacterial Properties of Cotinus coggygria from Turkey Pol. J. Environ. Stud. Vol. 22, No. 5 (2013), 1559-1561 3. Letícia Mendes Boppré, Julia Menezes Savaris, Emanuelle Catherine Maiola, Daniela Peressoni Vieira-Schuldt, Lucas da Fonseca Roberti Garcia, Cleonice da Silveira Teixeira, and Eduardo Antunes Bortoluzzi Can Heated Distilled Water Effectively Prevent Precipitate Formation between NaOCl and CHX? Int J Dent. 2024 Jan 6:2024:6612675. doi:10.1155/2024/6612675. eCollection 2024. 4. Tamar Shavadze, Natia Nizharadze, Marina Mamaladze, Ketevan Shalashvili, Eteri Kemertelidze Cotinus Coggygria Polyphenol Extract in Endodontics: Recent Results of Experimental Study Georgian Biomedical News DOI: 10.52340/GBMN.2024.01.01.91 5. L Lakshmi Narayanan, C Vaishnavi Endodontic microbiology J Conserv Dent. 2010 Oct;13(4):233-9. doi: 10.4103/0972-0707.73386. 6. Kaur, Kuljit; Kumar, Tarun; Mittal, Sunandan; Bansal, Renu Phytomedicine Herbal venture in green endodontics Endodontology / Volume 30 / Issue 2 / July December 2018 P. 98-102 7. Vishnuvardhini. S, Andamuthu Sivakumar, Vaiyapuri Ravi, A. S. Prasad and J.S.Sivakumar Herbendodontics–Phytotherapy In Endodontics: A Review Biomed Pharmacol J 2018;11(2) DOI : https://dx.doi.org/10.13005/bpj/1468 8. Mayada Jemâa, Dhiaedddine Shili, Sabrine Touaiti and MB Khattech Phytotherapy: A Complementary Approach in Endodontic Management Journal of Dentistry and Oral Care Medicine Volume 11 | Issue 1 ISSN: 2454-3276 Georgian Scientists/ . 7 N 4, 2025 666 9. Natia Nizharadze, Tamar Shavadze, Marine Mamaladze, Ketevan Shalashvili Antimicrobial screening of Cotinus Coggyggria Scop. Leaves extract for its application in endodontics https://doi.org/10.52340/jecm.2022.08.01 JECM 2022/8 10. Katarína Rendeková, Silvia Fialová, Lucia Jánošová, Pavel Mu aji and Lívia Slobodníková The Activity of Cotinus coggygria Scop. Leaves on Staphylococcus aureus Strains in Planktonic and Biofilm Growth Forms by Molecules 2016, 21(1), 50. 11. Dejan Stojkovi , Nina Dragi evi , Marija Ivanov, Nevena Gajovi , Milena Juriševi , Ivan Jovanovi , Marina Tomovi and Jelena Živkovi , New Evidence for Cotinus coggygria Scop. Extracts Application in Gastrointestinal Ailments by Pharmaceuticals 2025, 18(1), 98. 12. Gospodinova Z, Krasteva M. Cotinus coggygria Scop. leaf extract exerts high but not dose-and time-dependent in vitro cytotoxic activity on human breast cancer cells. Genet Plant Physiol. 2017;7:176-83. 13. VIII < > 1983 (207-213) 14. . . . , . 1976 , 3, 143-147 15. . . 2012 . . 16. ., ., . Cotinus coggygria Scop. – , , 2007, 3(4):451-460 17. . ., . ., . , . . Cotinus Coggygria m Alnus Barbata Chemical series volum 33 N4 tbilisi 2007 18. , , . Cotinus coggygria Scop. . - , 2011, (45) # 15, : (11) GE U 2011 1675 Y : (51) A 61 K 36/00 (IPC, 2006). . (8) 19. Giuliani, R., Bevilacqua, A., Corbo, M. R., & Severini, C. (2010). Use of microwave processing to reduce the initial contamination by Alicyclobacillus acidoterrestris in a cream of asparagus and effect of the treatment on the lipid fraction. Innovative Food Science & Emerging Technologies, 11(2), 328–334. https://doi.org/10.1016/j.ifset.2009.09.003 20. Behera, G., Sutar, P. P., & Aditya, S. (2017). Development of novel high power-short time (HPST) microwave assisted commercial decontamination process for dried turmeric powder (Curcuma Longa L.). Journal of food science and technology, 54(12), 4078–4091. https://doi.org/10.1007/s13197-017-2882-3 21. Jeevitha, G. C., Sowbhagya, H. B., & Hebbar, H. U. (2016). Application of microwaves for microbial load reduction in black pepper (Piper nigrumL.). Journal of the Science of Food and Agriculture, 96(12), 4243–4249. https://doi.org/10.1002/jsfa.7630 Georgian Scientists/ . 7 N 4, 2025 667 22. Sanja Matie, Snezana Stanie, Mirjana Mihailovie, Desanka Bogojevie Cotinus coggygria Scop.: An overview of its chemical constituents, pharmacological and toxicological potential Saudi Journal of Biological Sciences Volume 23, Issue 4, July 2016, Pages 452-461 23. OECD Guidelines for the Testing of Chemicals. Guideline #423. Strasbourg, 1998. 24. Finney, D. J., Ed. (1952). Probit Analysis. Cambridge, England, Cambridge University Press 25. Tamar Shavadze, Natia Nizharadze, Marina Mamaladze, Ketevan Shalashvili, Marine Sulakvelidze, Zhana Novikova, Karen Mulkijanyan, Eteri Kemertelidze. Comparative safety study of the phenolic constituents of smokebush (Cotinus coggygria) leaves. Georgian Biomedical News. 10 Apr 2023 Volume 1 (Issue 2):60-63 Application of Polyphenol (Tannin)-Rich Leaf Extracts of Cotinus coggygria Scop., Cultivated in Georgia, in Endodontic Practice. Abstract: In order to achieve effective and predictable outcomes in clinical endodontics, the search for novel therapeutic methods and agents remains highly relevant in contemporary dental practice. Considering the etiological factors of endodontic diseases, thorough and effective disinfection of the root canal system represents a fundamental prerequisite for successful treatment. The present study aims to evaluate the rationale for implementing an extract of Thrimli, a plant cultivated in Georgia, in endodontic practice. The selection of this agent was determined by its high content of polyphenolic compounds, which are known to provide broad-spectrum antimicrobial activity inherent to natural raw materials. This article reviews the results of all key preclinical in vitro experiments conducted as part of a retrospective framework for the continuation of in vivo and subsequent clinical investigations. Specifically, the study assesses the cytotoxicity, safety, allergenicity, biocompatibility, and local irritative effects of the experimental irrigating solution; evaluates its microbiological activity; determines the optimal extract fraction and solution concentration; and analyzes its compatibility with other commonly used endodontic irrigants. Keywords: Cotinus coggygria, polyphenols, antimicrobial activity, antibacterial activity, endodontics, pathogenic microflora, root canal system, Enterococcus faecalis.