INTRODUCTION Non-alcoholic fatty liver disease (NAFLD) consists of fat accumulation in the liver (hepatic steatosis) and affects about 1.8 billion people .NAFLD can progress towards non-alcoholic steato-hepatitis (NASH), which is a more severe condition characterized by necro-inammation with or without brosis. This can ultimately lead to cirrhosis and hepatocellular carcinoma (HCC). In addition, the related cardiovascular risk cannot be underestimated. To note, NAFLD is considered the hepatic manifestation of the metabolic syndrome, which is mainly characterized by obesity, dyslipidemia, insulin resistance, hypertension, and type 2 diabetes. In particular, in asymptomatic morbidly obese patients, there is a very high prevalence of approximately 90% of NAFLD (Adams LA, Angulo P et, al 2006) Non‐alcoholic fatty liver disease (NAFLD) encompasses a spectrum ranging from simple steatosis to non‐alcoholic steatohepatitis, which causes an increased risk of cirrhosis, type 2 diabetes, and cardiovascular complications. With the worldwide growing incidence of obesity, sedentary lifestyle, and unhealthy dietary pattern, NAFLD has currently been recognized as a major health burden. Dietary patterns and nutrients are the important contributors to the development, progression, and treatment of NAFLD and associated metabolic co morbidities. Generally, hyper caloric diet, especially rich in trans/ saturated fat and cholesterol, and fructose‐sweetened beverages seem to increase visceral adiposity and stimulate hepatic l ipid accumulat ion and progression into non‐alcoholic steatohepatitis, whereas reducing caloric intake, increasing soy protein and whey consumption, and supplement of monounsaturated fatty acids, omega‐3 fatty acids, and probiotics have preventive and therapeutic effects. In addition, choline, ber, coffee, green tea, and light alcohol drinking might be protective factors for NAFLD. Currently, NAFLD/NASH is the most common cause of liver disease worldwide and the third most common indication for liver transplantation in North America.1 The management of patients with NAFLD consists of treating steatohepatitis and the associated metabolic co morbidities (Jian‐Gao Fan et, al 2000) METHODOLOGY 100 samples were diagnosed denovo NAFLD were recruited as subjects in this study. Questionnaire method was used to collect data regarding General information such as-gender, age, income, family size, education, region, anthropometry, water consumption, past medical history of non- comm unicable problems, physical activity status, stress status etc., Thereafter, the condition of the subjects was assessed for every 3 months and 6months. The end results were evaluated, analyzed and maintained. Dietary intakes were assessed using 24hr recall method, Frequency of food consumption. The amounts consumed were recorded in house hold units by measuring with a ruler, katori and measuring cups. Records were validated according to corresponding food table and nutrient data base, nutritional values. Physical activity was assessed using 7-Day Physical activity diary. Participants were instructed to document all activities for 7 consecutive days. After every 3months and 6months the absolute minutes spent on various physical activities were calculated for each participant. Statistical analysis was completed and graphs were produced and obtained p-values and Chi-square signicance for majority of variables. RESULTS AND DISCUSSION TOTAL SUBJECT Fig.1. Distribution of subjects based on gender It is evident from Fig.1 that the majority of the subjects in the present study are females compared to the female population. About 56 % of female case studies are said to be having NAFLD when compared to the 44% of male case studies. Nonalcoholic fatty liver disease (NAFLD) has been recognized as a major health burden. Estimates suggest that about 20–30% of adults in developed countries have excess fat accumulation in the liver [Propst et al. 1995; Bellentani et al. 2000; Falck-Ytter et al. 2001; Bedogni and Bellentani, 2004; Zelber-Sagi et al. 2006], 50% among people with diabetes and about 80% in obese and morbidly obese people [Bellentani et al. 2000; Del Gaudio et al. 2002; Gupte et al. 2004] AGE GROUP Fig.2. Age group of the subjects NUTRITION STATUS AND NUTRIENT INTAKE OF DENOVO NAFLD PATIENTS Original Research Paper K. Vasundhara Padma R.D., M.SC., P.G. Diploma In Nutrition And Dietetics Clinical Research Non-alcoholic fatty liver disease (NAFLD) is one type of fatty liver which occurs when fat is deposited (steatosis) in the liver . It is a common and potential serious complication in the patient, who does not abuse or consume little to no alcohol. Non-alcoholic steatohepatitis (NASH) is the most extreme form of NAFLD. It is related to insulin resistance and the metabolic syndrome and may respond to treatment originally developed for other insulin-resistant states (e.g.: Diabetes mellitus type2) such as weight loss, metformin , and thiazolidinediones. It is marked by liver inammation, which might progress to scarring and irreversible damage. This damage is similar to the damage caused by heavy alcohol use. ABSTRACT KEYWORDS : Dr. Gowri Sankar* Pharm., Ph.D., FIC.*Corresponding Author VOLUME-9, ISSUE-1, JANUARY-2020 • PRINT ISSN No. 2277 - 8160 • DOI : 10.36106/gjra 20 X GJRA - GLOBAL JOURNAL FOR RESEARCH ANALYSIS Fig.2 shows that the majority of the subjects are between 40years to 50years of age. BODY MASS INDEX Fig.3. Classication of subjects based on BMI BMI was calculated as per their height and weight. Fig.3 shows majority of them are normal, only 39% of them are overweight and 16% of them are obese. DIETARY HABITS Fig.4. dietary habits of the selected subjects The above gure shows that the majority of them are non vegetarians, they are 76% high When compared to the vegetarians, who are 24%. There are no jains observed during this study. VOLUME-9, ISSUE-1, JANUARY-2020 • PRINT ISSN No. 2277 - 8160 • DOI : 10.36106/gjra PHYSICAL ACTIVITY DATA N ENERGY CARBOHYDRATES PROTEIN FAT INTIALLY 100 2626.5 ± 291.9 427.8 ± 46.8 64.4 ± 10.8 71.7 ± 12.3 RD3 MONTH 100 1550.5 ± 74.0 251.9 ± 12.0 56.5 ± 7.19 34.6 ± 1.8 TH6 MONTH 100 1406.5 ± 22.08 210.8 ± 2.9 70.2 ± 0.9 31.2 ± 0.4 ENERGY Fig.6. Energy (kcal/d) intakes of NAFLD patients CARBOHYDRATES Fig.7. Carbohydrates (kcal/d) intakes of NAFLD patients PROTEIN Fig.8. Protein (kcal/d) intakes of NAFLD patients FAT Fig.9. Fat (kcal/d) intakes of NAFLD patients The above gure shows the evident that there is a gradual increase in the physical activity like walking and running. Initially it was 31% and after an intervention in their diet and life style, NAFLD subjects are having an increase in their physical activity. TABLE.1. MACRO NUTRIENTS INTAKE OF DENOVO NAFLD PATIENTS TABLE.2. CLINICAL PARAMETERS OF THE NAFLD PATIENTS RANGE CLINICAL PARAMETERS BLOOD SUGARS (%) HTN+(%) LFT (%) FATTY LIVER (%) HYPERPIDEMIA (%) INITIALLY NORMAL 81 86 92 0 88 ABOVE NORMAL 19 14 8 100 12 RD3 MONTH NORMAL 91 96 98 13 95 ABOVE NORMAL 9 4 2 87 5 TH6 MONTH NORMAL 100 100 99 70 100 ABOVE NORMAL 0 0 1 30 0 Fig.5.The total mean of physical activity done by the NAFLD subjects X 21GJRA - GLOBAL JOURNAL FOR RESEARCH ANALYSIS VOLUME-9, ISSUE-1, JANUARY-2020 • PRINT ISSN No. 2277 - 8160 • DOI : 10.36106/gjra The above table.2. shows the clinical parameters of NAFLD patients . Initially the clinical parameters were high and in the rd3 month of intervention all the clinical parameters had slightly changed and improved. In the last intervention i.e. in ththe 6 month , the clinical parameters had been drastically changed when compared with the intial data. The results were highly satised. FATTY LIVER Fig.10. NAFLD patients with fatty liver normal and above normal Fig.10. the above graph explains the gradual change of the impression of fatty liver in the NAFLD pateints, who are abnormal intially, with the help of diet intervention,frequent coucelling on life style changes showed to normal levels. Perdomo et .al explained about the impact of nutritional changes on NAFLD is a major global health threat due to its growingincidence and prevalance . Theraputic aproaches emphasise lifestyle modications including phsical activity, healthy eating habbits that intend to controlbody weight and other factors. The purposeof this article is to assess the impact of dietary recommendations against NAFLD. During the study it was observed that, there was high consumption of simple sugars from soft drinks, soda, soda- pop, coca-cola etc., heavy intake of fructose from juices,jellies,jams, high fructosecorn syrup(HFCS) based products,and caramel colorant among the subjects, which allivited the risk for liver steatosis. Studies have assumed that fructose can be linked to NAFLD through both indirect and direct mechanisms. Indirectly, fructose may lead to adverese metabolic effects which can increase the risk of developing NAFLD.Directly, fructose might cause hepato toxic damage such as that observed inheriditary fructose intolerance. The evaluated group presents a health risk situation considering the indicators of nutritional status. The regular diet of the subjects appeared to be rich in carbohydrates-simple sugars, fructose, high calories, high fat etc., which triggers risk for NAFLD. CONCLUSION Counseling had a great impact on their life style, dietary modications and physical activity level. Patients were further advised to avoid consumption of rened processed foods which are high in fat(trans fat) and low ber content e.g. biscuits, cake, etc. Such foods are a source of empty calories and would worsen insulin resistance and may also promote weight gain. REFERENCES 1. Adams LA, Angulo P (2006). “Treatment of No-Alcoholic Fatty Liver Disease”. Post-graduation Medical Journal-82, (Page no: 315–22). 2. Bellentani S, Saccoccio G, Masutti F, Prevalence and risk factors for Hepatic steatosis in Northern Italy, Annual International Medical Journal 2000, 132: (Page no: 112-117). 3. Carolina M perdomo , gema fruhbeck and jevier escaladaimpact of nurtional changes on NAFLD. March -2019. 4. Chalasani N. et al. 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