TEXTINTRODUCTION: Obesity had emerged important risk factors associated with various disease. Prevalent of obesity is on the rise. In 2016, more than 1.9 billion adults aged 18 years and older were overweight. Of these over 650 million adults were obese. In 2016, 39% of adults aged 18 years and over (39% of men and 40% of women) were overweight. Overall, about 13% of the world's adult population (11% of men and 15% of women) were obese in 2016. The worldwide prevalence of obesity nearly tripled between 1975 and 2016. (1) Elevated LDL (>190 mg/dL) and triglycerides are risk factors for coronary heart disease. In all individuals, emphasize must be given on heart-healthy lifestyle across the life course. A healthy lifestyle reduces atherosclerotic cardiovascular disease risk at all ages. In younger individuals, healthy lifestyle can reduce development of risk factors and is the foundation of atherosclerotic cardiovascular disease risk reduction. In young adults 20 to 39 years of age, an assessment of lifetime risk facilitates the clinician–patient risk discussion and emphasizes intensive lifestyle efforts. In all age groups, lifestyle therapy is the primary intervention for metabolic syndrome. (2,3) Whole grain like wheat (Tritium aestivum Linn.) nds a place in the recommended DASH diet and is an important component of human diet, particularly in developing countries. Epidemiological studies reveal that consumption of whole grain and its products are protective against chronic diseases such as cardiovascular diseases, dyslipidemia, diabetes and cancer. Wheat when harvested as young green shoots germinated over a period of 6-10 days is generally called 'wheat grass' and it is known as 'functional food' during which vitamins, minerals and phenolic compounds such as avonoids are synthesized in wheat sprouts reaching maximum antioxidant potential.(4) Herbal products are highly acceptable and used 70 to 80% of the world population for their primary health care, especially in developing countries, due to their easy access, lesser side effects and low cost. It is also known to possess antioxidant as proven in several studies. (5,6) This property might protect the heart and preventing the most important cardiovascular complication. It may help in favourable lipid prole. This study will denitely help and add knowledge in scientic exploration of Effect of wheat grass (Tritium aestivum Linn) on lipid prole by observing its effect on Isoproterenol induced myocardial infarction in rats. SUBJECTS AND METHODS: This was a longitudinal study done on thirty healthy albino rats weighing between 100-200gms. Prior to the dietary manipulation, all rats were fed standard rat chow, containing 60% vegetable starch, 11% fat and 29% protein, water ad libitum and maintained on 12 hours light/dark cycle. Simultaneously rats were acclimatized to the procedure of blood pressure measurement daily for one week. DRUGS & CHEMICALS: The drugs selected for the study were obtained in pure powder form from the following sources. 1- Triticum aestivum powder –Girmes wheat grass, Pune 2- Carvediliol – Alkem Company, Mumbai, Maharastra, 400013 3- Isoproterenol (ISO) – Get well Pharmaceuticals, New Delhi Myocardial Infarction was induced in rats by intraperitoneal injection of Isoproterenol hydrochloride at a dose of 20mg/100g body weight, dissolved in normal saline for 2 consecutive days. Thirty albino rats were grouped into ve groups of six rats in each, and housed in separate cages group-wise, as follows. Group 1 (Control) : Rats were administered Normal saline 0.5ml orally for 15 day followed by Normal saline 5ml s.c. on th th14 & 15 day, at an interval of 24 hr. GROUP 2 (ISO) : Rats were administered Normal saline 0.5 ml orally once daily for 15 days followed by ISO 20mg/100g body weight th thsubcutaneously on the 14 & 15 day, at an interval of 24 hr. GROUP 3 (TR LOW DOSE + ISO) : Rats were pretreated with Triticum aestivum low dose 200mg/kg orally for a period of 15 days followed by subcutaneous injection of Isoproterenol 20mg/100g body EFFECT OF WHEAT GRASS (TRITIUM AESTIVUM LINN) ON LIPID PROFILE IN ALBINO RATS INDUCED WITH MYOCARDIAL INFARACTION. Original Research Paper Dr Annapoorna Sahoo Tutor, Pharmacology Department, Bhima-bhoi Medical College And Hospital, Balangir, Odisha X 51GJRA - GLOBAL JOURNAL FOR RESEARCH ANALYSIS Pharmacology Context: Obesity had emerged important risk factors associated with various disease. Prevalent of obesity is on the rise Aims: to study the effect of wheat grass (Tritium aestivum Linn) on lipid prole by observing its effect on Isoproterenol induced myocardial infarction in rats. Settings and Design: This was a longitudinal study done on thirty healthy albino rats Methods and Material: Thirty albino rats were grouped into ve groups of six rats in each, and housed in separate cages group- wise. All groups except control received Isoproterenol. In addition to Isoproterenol, two groups were given wheat grass at different dosage and one group received Carvediliol. Serum lipid prole was assessed. Statistical analysis used: ANOVA test and post hoc Dunnett test using SPSS version 16, unpaired t testResults: Signicant reduction in HDL and increse in LDL, cholesterol and triglyceride was seen.. Conclusions: Triticum aestivum has a favourable effect on lipid prole. Wheatgrass extract can be a good alternative or primary therapy in treating dyslipidemia. ABSTRACT KEYWORDS : Tritium Aestivum Linn, Dyslipidemia Dr Pravin Dhone* Professor And Head, Bhima-bhoi Medical College And Hospital, Balangir, Odisha *Corresponding Author VOLUME-8, ISSUE-12, DECEMBER-2019 • PRINT ISSN No. 2277 - 8160 • DOI : 10.36106/gjra 52 X GJRA - GLOBAL JOURNAL FOR RESEARCH ANALYSIS th thweight on 14 & 15 day at an interval of 24 hr. GROUP 4 (TR HIGH DOSE + ISO): Rats were pretreated with Triticum aestivum high dose 250mg/kg orally for a period of 15 days followed by subcutaneous injection of Isoproterenol 20mg/100g body th thweight on 14 & 15 day at an interval of 24 hr. GROUP 5 (CARVEDILOL + ISO): Rats were pretreated with carvedilol 5mg/kg orally for a period of 15 days followed by subcutaneous injection of th thIsoproterenol 20mg/100g body weight on 14 and 15 day at an interval of 24hr. BIOCHEMICAL STUDIES : Serum lipid prole (cholesterol, triglyceride, HDL, LDL) will also be estimated in all the groups. This was done by standard acceptable method. (7) Statistical Analysis--The comparison were done using ANOVA test and post hoc Dunnett test using SPSS version 16, unpaired t test and p<0.05 was considered as statistically signicant. RESULTS: Effect of Triticum aestivum linn. on various lipid prole is shown in in Isoproterenol induced myocardial infarction in albino rats. As evident in the table, it is having comparable effect to that of Carvedilol. Wheat grass (WG) had signicantly increased the level of HDL and it has signicantly decreased the level of cholesterol, LDL and triglycerides. We also dose dependant result. (Table 1) VOLUME-8, ISSUE-12, DECEMBER-2019 • PRINT ISSN No. 2277 - 8160 • DOI : 10.36106/gjra Group Drug Dose Duration Induction of MI 1 Distilled Water 0.5 ml For 15 days No treatment 2 Distilled Water 0.5 ml Isoproterenol 20mg/100gbwt. SC Inj on 14th& 15th day. 3 Triticum aestivum Extract 200mg/ kg Parameters (mmHg) Group1 CONTROL (Distilled Water) Group2 CONTROL ISO 20mg/100gbwt Group3 WG200mg/kg +ISO 20mg/100gbwt Group4 WG250mg/kg +ISO 20mg/100gbwt Group5 Carvedilol 5mg/kg+ ISO 20mg/100gbwt Cholesterol ± SE 54.43 ±4.145 109.16*** ±4.88 90.49* ±5.52 67.73*** ±5.91 56.96 ±3.53 Triglyceride ± SE 132.32 ± 5.529 317.07*** ±13.5 247.405* ±7.45 178.638*** ±8.112 127.77 ±4.867 HDL ± SE 27.135 ±1.87 33.14*** ±1.51 34.847* ±1.93 38.98*** ±2.14 41.03*** ±1.35 LDL ± SE 66.97 ±0.59 39.9*** ±0.66 59.06* ±0.66 51.97*** ±0.6 44.99*** ±0.59 Table 1: Effect of Triticum aestivum Linn. on lipid prole. 4 Triticum aestivum Extract 250mg/ kg 5 Carvedilol 5mg/kg DISCUSSION: This study was carried out to nd out Effect of wheat grass (Tritium aestivum Linn) on lipid prole by observing its effect on Isoproterenol induced myocardial infarction in rats. Wheat grass (WG) had signicantly increased the level of HDL and it has signicantly decreased the level of cholesterol, LDL and triglycerides. We also dose dependant result. Wheat grass refers to the young grass of wheat (Triticum aestivum) germinated for a period of 6 to 10 days. It contains vitamin C and E, ß carotene, ferulic acid, vanilic acid and phenols, especially avonoids. Wheat grass juice is found to have healing properties in various degenerative diseases and is known to benet blood cells, bones, glands, kidney and other parts of the body. (8,9) Since little or no work has been done on cardio-protective effects of wheat grass, the present study was designed to analyse its role on Isoproteronol induced myocardial insufciency. In the study , rst myocardial-infarction was induced with a �- adrenergic agonist Isoproterenol, which caused severe stress in the myocardium causing coagulative necrosis (i.e. infarct like) of heart muscle. Pre-treatment of Triticum aestivum linn.in both doses (TA Linn + ISO) showed a reduction in blood lipid prole levels with concomitantly increase in HDL cholesterol. Lipid lowering effect of Triticum aestivum linn.is due to the inhibition of cholesterol biosynthesis, increased fecal bile acid secretion and stimulation of receptor mediated catabolism of LDL cholesterol and increase in the uptake of LDL from blood by the liver. Heart tissue injury induced by ISO in rats was indicated by elevated level of the marker enzymes such as serum. (10,11,12) Wheat grass by virtue of its avonoids and phenolics, effectively protected the heart and vessels from induced damage. Hence Wheat grass extract can become an effective therapeutic agent for treatment of dyslipidemia. To conclude, the present result suggests that Triticum aestivum has a favourable effect on lipid prole. Wheatgrass extract can be a good alternative or primary therapy in treating dyslipidemia. . 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