HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY Vol. 50 pp. 23–28 (2022) hjic.mk.uni-pannon.hu DOI: 10.33927/hjic-2022-05 PRODUCTION OF CHIRAL (S)-2-PHENYL-1-PROPANOL BY ENANTIOSE- LECTIVE BIOCATALYSTS PIROSKA LAJTAI-SZABÓ *1 , TÍMEA BRIGITTA BAGÓ1 , AND NÁNDOR NEMESTÓTHY1 1Research Group on Bioengineering, Membrane Technology and Energetics, University of Pannonia, Egyetem u. 10, Veszprém, 8200, HUNGARY Enantioselective production of (S)-2-phenyl-1-propanol is important as in order to be applied in industry, a high degree of optical purity is required. Besides organocatalysts and metal complexes, biocatalysts can be used for its synthesis in their isolated form or as whole-cell biocatalysts, both of which have various advantages and disadvantages. In this research, Saccharomyces cerevisiae, as a whole-cell biocatalyst, and recombinant horse-liver alcohol dehydrogenase (ADH), as an isolated enzyme, were investigated in terms of their activity, kinetics and enantioselectivity. In the case of yeast, the rate of cofactor regeneration was twice that of substrate conversion, moreover, the biocatalyst Saccharomyces cerevisiae can be characterised by substrate-limited kinetics and low enantioselectivity. In contrast, the isolated enzyme recombi- nant horse-liver ADH exhibited biphasic kinetics and cofactor regeneration was the rate-limiting step. The outstanding enantioselectivity of recombinant horse-liver ADH renders it a promising catalyst for the purpose of this synthesis. Keywords: alcohol dehydrogenase, whole-cell biocatalyst, Saccharomyces cerevisiae 1. Introduction 2-phenyl-1-propanol is a fragrance ingredient that pro- duces the Lila-hyacinth odour commonly used in cosmet- ics, fine fragrances and household cleaners [1]. Besides, it is the basis of some non-steroidal anti-inflammatory drugs and the precursor of other fragrances [2]. These fields of use require a high degree of optical purity since the enantiomers of the compound, by and large, bring about different biological effects [3]. Asymmetric syntheses are preferentially obtained us- ing enzymes, given the capability of most to catalyse or- ganic reactions with high enantioselectivity under mild conditions [4, 5]. Besides isolated enzymes, whole cells are also being applied more and more often as biocat- alysts, given the disparate attributes of both. Whole- cell biocatalysts ensure the optimal environment for the enzyme, thereby providing a quite stable system. Fur- thermore, they contain cofactors and are able to bring about cofactor regeneration without the necessary ad- dition of any other compounds [6]. However, the pres- ence of a variety of enzymes may lead to side reac- tions. Also, isozymes with different enantiomeric pref- erences may lower the overall enantiomeric excess into Recieved: 11 March 2022; Revised: 21 March 2022; Accepted: 21 March 2022 *Correspondence: lajtai-szabo.piroska@mk.uni-pannon.hu the bargain [7]. Unlike whole-cell biocatalysts, isolated enzymes improve the level of control over the process in the absence of side reactions, thereby enhancing its re- producibility. In addition, inhibition is less likely to oc- cur because of the greater degree of tolerance concerning the concentrations of both the substrate and product. On the other hand, the provision of a cofactor and its regen- erating system significantly increases costs. In order to choose the optimal catalyst for a given synthesis, a de- tailed comparison of their advantages and disadvantages, e.g. attainable yield, productivity, product purity, required downstream processes and costs, should be made [8]. In this research, two types of alcohol dehydrogenases (ADH) were investigated with regard to the conversion of racemic 2-phenylpropionaldehyde to (S)-2-phenyl-1- propanol. For the catalysis, the cofactor nicotinamide adenine dinucleotide (NADH) is required by the enzyme which has to be regenerated in order to ensure continuous product formation. Ethanol was applied as an auxiliary substrate for the regeneration which was catalysed by the ADH. The reaction schemes are presented in Fig. 1. Be- cause of the low solubility of the substrate and the product in aqueous media, a two-phase system was applied. Saccharomyces cerevisiae was applied in dried form (instant baker’s dry yeast) as a whole-cell biocatalyst. Over recent decades, both wild and genetically modified strains of yeast have been gaining more and more atten- tion as biocatalysts in the production of fine chemicals [7]. Although (S)-alcohol is generally the predominant https://doi.org/10.33927/hjic-2022-05 mailto:lajtai-szabo.piroska@mk.uni-pannon.hu 24 LAJTAI-SZABÓ, BAGÓ AND NEMESTÓTHY Figure 1: Reaction scheme enantiomer in the reduction of racemic carboxylic acid compounds by applying yeast [6], this is dependent on the given substrate [9]. Our second catalyst was recombinant horse-liver ADH expressed in E. coli. Isolated recombi- nant horse-liver ADH is frequently applied for asymmet- ric syntheses [10–12], moreover, its variant expressed in bacteria can be a cheaper and more accessible alternative. The aim of this research was to characterise the afore- mentioned biocatalyst in terms of activity, kinetics and enantioselectivity, thereby enabling their critical compar- ison. In addition, mass transfer through the organic-water interphase was also investigated in order to characterise the relationship between the rates of each step. 2. Experimental Methods 2.1 Applied chemicals All chemicals were commercially available and used without further purification. Diisopropyl ether (puriss), ethyl alcohol (a.r.), dodecane (a.r.), trifluoroacetic an- hydride (98%), racemic 2-phenylpropionaldehyde (98%) and S-(2)-phenyl-1-propanol (97%) were purchased from Sigma-Aldrich. Recombinant horse-liver alcohol dehy- drogenase (expressed in E. coli) as well as lyophilised powder (1.5 U/mg) and β-nicotinamide adenine dinu- cleotide (sodium salt, 98%) were obtained from Sigma- Aldrich and Thermo Fisher Scientific, respectively. K2HPO4, KH2PO4 and Na2CO3 were purchased from VWR, while instant baker’s dry yeast was purchased from a local store. 2.2 Activity measurements A spectrophotometric method was used to determine the catalytic activity characteristic of cofactor regeneration. Given that the maximum adsorption of NADH is at 340 nm while that of NAD+ is negligible at this wavelength, conversion of the cofactor can be followed by the change in the wavelength of adsorption. In the case of isolated ADH, 10 µL of enzyme solution (10 mg/mL), 600 µL of NAD+ solution (4 mg/mL) and 1240 µL of buffer so- lution were mixed in a quartz cuvette. The reaction was initiated by the addition of 150 µL cc. of ethyl alcohol and the change in absorbance was measured at 340 nm by a Shimadzu UV-1800 ultraviolet-visible spectropho- tometer. In the case of the whole-cell biocatalyst, 10 µL of an instant yeast suspension (10 mg/mL) was applied instead of an enzyme solution. The catalytic activity was calculated based on the following equation: VA = dA dt Vcuvette �d Venzyme h (1) where VA denotes the volume activity [U/cm3], dA dt rep- resents the gradient of the line (by plotting absorbance vs. time), Vcuvette stands for the volume of the mix- ture [cm3], � refers to the molar extinction coefficient of NADH at 340 nm [6.22 cm2/µmol], d is the width of the cuvette [1 cm], Venzyme denotes the volume of the en- zyme solution [cm3] and h represents dilution. The catalytic activity characteristic of the conversion of 2-phenylpropionaldehyde cannot be measured by a spectrophotometer since the reaction mixture consists of two phases. Therefore, the catalytic activity characteris- tic of the whole process (substrate conversion, cofactor regeneration and mass transfer through the organic-water interface) was calculated from the results gained by mea- suring the kinetics, as described in Sec. 2.4. 2.3 Mass-transfer rate Since experimental conditions applied by conversion measurements are unsuitable for determining the mass- transfer rate through organic-water interface, a simpli- fied system was applied for this purpose [13]. 13.4 mg of 2-phenylpropionaldehyde and 18.5 mg of dodecane, which served as an internal standard for gas chromatog- raphy (GC) analysis, were dissolved in 10 mL of diiso- propyl ether. A Schott glass bottle was filled with 6 L of distilled water and the organic solution carefully poured onto the surface of the water phase. During the following 27 hours, samples were taken from the organic phase and analysed by GC. (The parameters of chromatographic analysis were the same as described in Sec. 2.4). The ratio of aldehyde to dodecane was plotted as a function of time and a kinetic model was fitted to the mea- sured data in accordance with the following equation: J = d(c0 − acw) (2) where J denotes mass transfer through the interface [mol/(min cm2)], d represents the mass transfer coeffi- cient [cm/min], c0 stands for the initial substrate concen- tration in the organic phase [mol/cm3], cw refers to the substrate concentration in the water phase [mol/cm3] and a is the ratio of the activity coefficients. The difference between the measured and calculated data was minimized by the Excel Solver plug-in. Although the water phase was gently mixed by a magnetic stirrer, the water–organic interface was stationary. Therefore, its surface can be re- garded as a constant. The mass transfer rate can be calcu- lated by dividing the mass transfer (J) by the interfacial area. Hungarian Journal of Industry and Chemistry PRODUCTION OF CHIRAL (S)-2-PHENYL-1-PROPANOL 25 Table 1: Heating program Ramp rate [◦C/min] Temperature [◦C] Hold time [min] 70 25 1 110 0 20 180 2 2.4 Kinetics In all the experiments, 7 cm3 of organic solvent and 7 cm3 of phosphate buffer (75 mM, pH 8.0) were used. The molar ratio of 2-phenylpropionaldehyde to ethyl alcohol was set at 3.7, based on data from the literature [14]. In the case of isolated ADH, 60 µL of NADH solution (7.5 mg/mL, freshly made with a buffer solution) was added to the reaction media. (The optimal amount of cofactor was determined during preliminary measurements, however, this data is not shown.) In the case of instant baker’s dry yeast, since the cell contained a sufficient amount of co- factor, no further addition of NADH was necessary. The reactions were initiated by adding the catalyst – 300 mg of instant baker’s dry yeast or 500 µL of enzyme solution (10 mg/mL, freshly made with a buffer solution). The re- action mixtures were shaken in a thermostatic incubator (IKA KS 4000 i control) at 200 rpm and 30 ◦C. To inves- tigate the product formation, samples were taken from the organic phase and analysed by an HP 5890 gas chromato- graph (140 ◦C isothermal). The GC was equipped with a DB-FFAP column (1 µm × 30 m × 0.53 mm, Agilent Technologies) and a flame ionisation detector. 2.5 Enantioselectivity While measuring the enantioselectivity, the content of the reaction mixture and operational parameters were the same as described in Sec. 2.3. Before GC analysis, pre- liminary derivatization was required. 1 mL of trifluo- roacetic anhydride and 1 mL of diisopropyl ether were added to 500 µL of the sample while heating the mix- ture under reflux at 70 ◦C for 30 mins. Once the reaction mixture had been cooled to room temperature, it was neu- tralized with 4 mL of Na2CO3 (20%) and a sample from the organic phase analysed by a Shimadzu GC-2014 gas chromatograph equipped with a LIPODEX E capillary column (0.2 µm × 25 m × 0.25 mm, Macherey-Nagel) and a flame ionisation detector. Table 1 contains the parameters of the applied heat- ing program. The peaks were deconvoluted by OriginPro software to fit the Gaussian curves. To identify the peaks of the enantiomers, derivatization and analysis was per- formed using pure S-(2)-phenyl-1-propanol. The reten- tion time of the derivative of the product was 52.5 mins. 3. Results and Evaluation 3.1 Kinetics By plotting the amount of product as a function of time, a line can be fitted to the initial linear phase of the graph and its gradient is the initial rate of reaction as a result of the given substrate concentration. A kinetic curve re- sults from repeating the method with different initial sub- strate concentrations, yielding information about the de- pendence of the reaction rate on it. In the case of whole-cell biocatalysts, the prod- uct formation can be described by the single-substrate Michaelis-Menten model as the amount of cofactor can be considered to be constant due to its fast regeneration (see Sec. 2.3). In the region of lower substrate concentrations, first- order kinetics was observed as expected (Fig. 2). How- ever, at 0.267 M, the curve peaked followed by a rela- tively steady decrease instead of phase saturation. As a result, it can be concluded that higher amounts of sub- strate limit enzymatic conversion, therefore, 0.267 M is the optimal initial concentration to maximise the reaction rate. In contrast to yeast cells, by applying recombinant ADH, the reaction rate of cofactor regeneration may limit product formation (see Section 3.2), therefore, cannot be neglected. Since two reactions involved multiple sub- strates catalysed simultaneously by the same enzyme, the kinetics did not follow the Michaelis-Menten model. Al- ternatively, a biphasic model was applied which divides the curve into two phases: at low substrate concentra- tions, the enzyme’s affinity is relatively high while the turnover number is low. On the contrary, a low affinity and high turnover number is characteristic of the region of high substrate concentration (Fig. 3). Biphasic kinetics can be modelled by the following equation [15]: V = Vmax1 [S] + CLint[S] 2 KM1 + [S] (3) where Vmax1 as well as KM1 describe the first high affinity–low-turnover phase and CLint – equal to the ra- tio Vmax2 : KM2 – denotes the second low-affinity–high- turnover phase. Figure 2: Kinetics of the whole-cell biocatalyst 50 pp. 23–28 (2022) 26 LAJTAI-SZABÓ, BAGÓ AND NEMESTÓTHY Figure 3: Biphasic enzyme kinetics [15] Figure 4: Kinetics of the isolated enzyme Apart from one exception (at 0.22 M), since model data calculated using Eq. 3 fitted well to the measured data (Fig. 4), biphasic kinetics is presumably a suitable model to describe the kinetics of an isolated enzyme. Having been minimized by the Excel Solver plug-in, the model parameters were the following: Vmax1 = 7.523; CLint = 3.458; KM1 = 0.464. According to Manevski [15], biphasic kinetics may imply the presence of multiple substrate binding sites. However, the applied methods were unsuitable for further investigating the underlying mechanisms of the reactions taking place. 3.2 Activity The activity of the catalysts was measured during both cofactor regeneration and the process as a whole (Table 2). One Unit (U) stands for the amount of catalyst nec- essary to produce 1 µmol of product in 1 minute under the measurement parameters. (Substrate conversion could not be investigated separately as previously mentioned in Sec. 2.2). Although normally the catalytic activity should be measured in the saturation phase of the kinetics, none of the kinetic curves enabled this. Therefore, measure- ments were made at a substrate concentration of 0.27 M, which is the substrate concentration at which the kinetic Table 2: Activity of the catalysts yeast cell [U/mg] recombinant ADH [U/mg] cofactor regeneration 0.19 0.05 complete reaction (0.27 M) 0.02 0.7 complete reaction (1.08 M) – 1.64 curve of the yeast-cell catalyst is at its maximum. In the case of the isolated enzyme, the activity was measured at the same concentration as that of yeast in order to com- pare the two catalysts. This was also determined at the highest measured point of the kinetic curve, namely at 1.08 M. Based on the results, cofactor regeneration is one scale faster when applying a whole-cell biocatalyst in- stead of an isolated enzyme. Although the same enzyme catalyses both substrate conversion and cofactor regen- eration when isolated ADH is used, yeast cells contain several enzymes that are capable of participating in re- generation reactions which can occur more rapidly as a result. On the other hand, the overall reaction rate using the same substrate concentration was 35 times higher when recombinant ADH was used and further increases in sub- strate concentration resulted in even higher reaction rates. 3.3 Mass transfer through the organic–water interface In order to determine the rate-limiting step of the whole process, the mass transfer rate through an organic–water interface was investigated. Owing to simplifications of the measurement and the imprecise nature of model fit- ting, the calculated mass transfer rate may be some- what inaccurate. Nevertheless, the goal was to estimate its order of magnitude rather than determining its precise value. Based on the kinetic model fitted to the measured data (Fig. 5), the mass transfer rate was calculated to be 1.33 · 10−5 mol/min. By comparing this value with the Figure 5: Mass transfer process Hungarian Journal of Industry and Chemistry PRODUCTION OF CHIRAL (S)-2-PHENYL-1-PROPANOL 27 Table 3: Enantiomeric excess (ee) and degree of conversion from studies on the production of (S)-2-phenyl-1-propanol Catalyst Solvent Substrate conc. Product, degree of conversion ee (S) Ref. horse-liver ADH 0.24 U/mL buffer, 165 mM 41 mM, 25 % (2 h) 91 % [14]isopropyl ether (63 % v/v) 82 mM, 50 % (24 h) 88 % horse-liver ADH 0.01 mg/mL buffer pH= 7.5, CH3CN (10 % v/v) 0.5 mM 0.36 mM, 72 % (5 h) 78 % [12] recombinant horse-liver ADH, exp. in E. coli 0.5 U/mL buffer pH= 8.0, diisopropyl ether (1:1 v/v) 267 mM 45 mM, 17 % (1 h) 100 % this work CtXR D51A mutant E. coli, 4 gCDW/L buffer pH= 7.5 100 mM 41 mM, 41 % (2 h) 95 % [2] whole-cell 40 gCDW/L 70 mM, 70 % (2 h) 45 % S. cerevisiae, 43 gCDW/L buffer pH= 8.0, 267 mM 74 mM, 28 % (1 h) 24 % this work whole-cell diisopropyl ether (1:1 v/v) 345 mM 55 mM, 16 % (1 h) 36 % reaction rates of both catalysts, it could be established that the mass transfer rate is two or three times faster than those of product formation or cofactor regeneration. Therefore, the rate-limiting step of the whole process is cofactor regeneration and substrate conversion when an isolated enzyme and whole-cell biocatalyst is applied, re- spectively. 3.4 Enantioselectivity The enantioselectivity of the whole-cell biocatalyst was measured at three different initial substrate concentra- tions: at the maximum of the kinetic curve (0.267 M) as well as at two higher values (0.345 M and 0.746 M) to ex- amine whether increasing the substrate concentration is beneficial as far as achieving optical purity is concerned. The enantiomeric ratio was calculated from the ratio of the peak areas at 0.5, 1.0 and 1.5 hours (Fig. 6). Changes in the enantiomeric ratio as the reaction pro- gressed were insignificant, moreover, the difference in the reaction time between 0.345 M and 0.746 M was negligi- ble. At 0.267 M, the final result was 0.62, while the best result, that is, 0.68, was achieved at 0.746 M. As a result, Figure 6: The enantioselectivity of the whole- cellbiocatalyst it could be established that to achieve an optimal reac- tion rate and enantioselectivity, different initial substrate concentrations are required. In the case of the isolated enzyme, the enantioselec- tivity was measured at the same substrate concentrations as when the whole-cell biocatalyst was applied. However, since only one peak corresponding to the (S)-enantiomer of the derivative was detected, further analysis was un- necessary and the enantioselectivity regarded as 100%. As in terms of consumption optical purity is a key concern, the results of this work were compared with some data from the literature (Table 3). In the case of whole-cell biocatalysts, the results of Rapp et al. [16] are more favourable than ours. However, since both stud- ies suggest that the maximum degree of conversion and enantiomeric excess cannot be achieved simultaneously, a compromise must be made. In the case of the isolated enzyme, the degree of conversion in this study is promis- ing if the reaction time is also taken into account. As for the enantiomeric excess, our result is clearly outstanding, therefore, recombinant horse-liver ADH provides a satis- factory alternative for catalysing the production of (S)-2- phenyl-1-propanol. 4. Conclusion In this work, the conversion of racemic 2- phenylpropionaldehyde into (S)-2-phenyl-1-propanol was investigated by applying a whole-cell biocatalyst (Saccharomyces cerevisiae) and an isolated enzyme (recombinant horse-liver ADH expressed in E. coli). Significant differences were observed between the cat- alysts in terms of all the considered parameters. Firstly, the whole-cell biocatalyst exhibited substrate-limited kinetics, while the isolated enzyme could be described by biphasic kinetics. The yeast cell contained a sufficient amount of cofactor for the reaction, moreover, its regen- eration was twice as fast as in the case of the isolated 50 pp. 23–28 (2022) 28 LAJTAI-SZABÓ, BAGÓ AND NEMESTÓTHY enzyme. Therefore, the whole-cell biocatalyst is more beneficial from this point of view. On the other hand, the enzymatic activity of the whole process was at least 35 times higher when recombinant horse-liver ADH was applied and could be further enhanced by increasing the initial substrate concentration. Most importantly, the isolated enzyme catalysed the conversion with 100 % enantioselectivity, which is also clearly outstanding compared to data from the literature. In conclusion, although recombinant horse-liver ADH is more expen- sive, it is definitely a more efficient catalyst than yeast as a whole-cell biocatalyst. Therefore, recombinant horse-liver ADH is a promising biocatalyst with regard to the synthesis of (S)-2-phenyl-1-propanol. 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