id sid tid token lemma pos hjic-799 1 1 4 4 NUM hjic-799 1 2 hanáková hanáková NOUN hjic-799 1 3 , , PUNCT hjic-799 1 4 potěšil potěšil PROPN hjic-799 1 5 , , PUNCT hjic-799 1 6 bernatík bernatík ADJ hjic-799 1 7 , , PUNCT hjic-799 1 8 červenka červenka PROPN hjic-799 1 9 , , PUNCT hjic-799 1 10 rádsetoulal rádsetoulal PROPN hjic-799 1 11 , , PUNCT hjic-799 1 12 bryja bryja NOUN hjic-799 1 13 , , PUNCT hjic-799 1 14 and and CCONJ hjic-799 1 15 zdráhal zdráhal NOUN hjic-799 1 16 figure figure VERB hjic-799 1 17 1 1 NUM hjic-799 1 18 : : PUNCT hjic-799 1 19 comparison comparison NOUN hjic-799 1 20 of of ADP hjic-799 1 21 phosphorylation phosphorylation NOUN hjic-799 1 22 sites site NOUN hjic-799 1 23 of of ADP hjic-799 1 24 hdvl3 hdvl3 NOUN hjic-799 1 25 induced induce VERB hjic-799 1 26 by by ADP hjic-799 1 27 ck1ε ck1ε PROPN hjic-799 1 28 and and CCONJ hjic-799 1 29 nek2 nek2 PROPN hjic-799 1 30 . . PUNCT hjic-799 2 1 experimentally experimentally ADV hjic-799 2 2 determined determine VERB hjic-799 2 3 phosphorylation phosphorylation NOUN hjic-799 2 4 sites site NOUN hjic-799 2 5 by by ADP hjic-799 2 6 ck1ε ck1ε PROPN hjic-799 2 7 and and CCONJ hjic-799 2 8 nek2 nek2 NOUN hjic-799 2 9 are be AUX hjic-799 2 10 indicated indicate VERB hjic-799 2 11 by by ADP hjic-799 2 12 green green ADJ hjic-799 2 13 background background NOUN hjic-799 2 14 color color NOUN hjic-799 2 15 . . PUNCT hjic-799 3 1 phosphorylation phosphorylation NOUN hjic-799 3 2 sites site NOUN hjic-799 3 3 identified identify VERB hjic-799 3 4 only only ADV hjic-799 3 5 by by ADP hjic-799 3 6 nek2 nek2 NOUN hjic-799 3 7 are be AUX hjic-799 3 8 indicated indicate VERB hjic-799 3 9 by by ADP hjic-799 3 10 red red ADJ hjic-799 3 11 background background NOUN hjic-799 3 12 color color NOUN hjic-799 3 13 . . PUNCT hjic-799 4 1 analyzed analyze VERB hjic-799 4 2 by by ADP hjic-799 4 3 liquid liquid ADJ hjic-799 4 4 chromatography chromatography NOUN hjic-799 4 5 coupled couple VERB hjic-799 4 6 with with ADP hjic-799 4 7 mass mass ADJ hjic-799 4 8 spectrometry spectrometry NOUN hjic-799 4 9 ( ( PUNCT hjic-799 4 10 lc lc PROPN hjic-799 4 11 - - PUNCT hjic-799 4 12 ms ms PROPN hjic-799 4 13 / / SYM hjic-799 4 14 ms ms NOUN hjic-799 4 15 ) ) PUNCT hjic-799 4 16 for for ADP hjic-799 4 17 protein protein NOUN hjic-799 4 18 identification identification NOUN hjic-799 4 19 ( ( PUNCT hjic-799 4 20 i i NOUN hjic-799 4 21 d d PROPN hjic-799 4 22 run run PROPN hjic-799 4 23 ) ) PUNCT hjic-799 4 24 . . PUNCT hjic-799 5 1 the the DET hjic-799 5 2 rest rest NOUN hjic-799 5 3 of of ADP hjic-799 5 4 the the DET hjic-799 5 5 peptide peptide NOUN hjic-799 5 6 mixture mixture NOUN hjic-799 5 7 was be AUX hjic-799 5 8 used use VERB hjic-799 5 9 for for ADP hjic-799 5 10 phosphopeptide phosphopeptide NOUN hjic-799 5 11 analysis analysis NOUN hjic-799 5 12 . . PUNCT hjic-799 6 1 ms ms PROPN hjic-799 6 2 phosphomix phosphomix PROPN hjic-799 6 3 1 1 NUM hjic-799 6 4 , , PUNCT hjic-799 6 5 2 2 NUM hjic-799 6 6 , , PUNCT hjic-799 6 7 3 3 NUM hjic-799 6 8 light light NOUN hjic-799 6 9 ( ( PUNCT hjic-799 6 10 sigma sigma PROPN hjic-799 6 11 aldrich aldrich PROPN hjic-799 6 12 ) ) PUNCT hjic-799 6 13 was be AUX hjic-799 6 14 added add VERB hjic-799 6 15 to to ADP hjic-799 6 16 the the DET hjic-799 6 17 samples sample NOUN hjic-799 6 18 before before ADP hjic-799 6 19 the the DET hjic-799 6 20 phosphopeptide phosphopeptide NOUN hjic-799 6 21 enrichment enrichment NOUN hjic-799 6 22 step step NOUN hjic-799 6 23 in in ADP hjic-799 6 24 a a DET hjic-799 6 25 concentration concentration NOUN hjic-799 6 26 of of ADP hjic-799 6 27 0.1 0.1 NUM hjic-799 6 28 pmol pmol NOUN hjic-799 6 29 . . PUNCT hjic-799 7 1 phosphopeptides phosphopeptide NOUN hjic-799 7 2 were be AUX hjic-799 7 3 enriched enrich VERB hjic-799 7 4 using use VERB hjic-799 7 5 a a DET hjic-799 7 6 pierce pierce PROPN hjic-799 7 7 magnetic magnetic PROPN hjic-799 7 8 titanium titanium PROPN hjic-799 7 9 dioxide dioxide NOUN hjic-799 7 10 phosphopeptide phosphopeptide NOUN hjic-799 7 11 enrichment enrichment NOUN hjic-799 7 12 kit kit NOUN hjic-799 7 13 ( ( PUNCT hjic-799 7 14 thermo thermo NOUN hjic-799 7 15 fisher fisher PROPN hjic-799 7 16 scientific scientific PROPN hjic-799 7 17 , , PUNCT hjic-799 7 18 waltham waltham PROPN hjic-799 7 19 , , PUNCT hjic-799 7 20 massachusetts massachusetts PROPN hjic-799 7 21 , , PUNCT hjic-799 7 22 usa usa PROPN hjic-799 7 23 ) ) PUNCT hjic-799 7 24 according accord VERB hjic-799 7 25 to to ADP hjic-799 7 26 the the DET hjic-799 7 27 protocol protocol NOUN hjic-799 7 28 of of ADP hjic-799 7 29 the the DET hjic-799 7 30 manufacturer manufacturer NOUN hjic-799 7 31 and and CCONJ hjic-799 7 32 eluted elute VERB hjic-799 7 33 into into ADP hjic-799 7 34 an an DET hjic-799 7 35 autosampler autosampler ADJ hjic-799 7 36 vial vial NOUN hjic-799 7 37 . . PUNCT hjic-799 8 1 the the DET hjic-799 8 2 solution solution NOUN hjic-799 8 3 was be AUX hjic-799 8 4 concentrated concentrate VERB hjic-799 8 5 under under ADP hjic-799 8 6 a a DET hjic-799 8 7 vacuum vacuum NOUN hjic-799 8 8 to to ADP hjic-799 8 9 a a DET hjic-799 8 10 volume volume NOUN hjic-799 8 11 of of ADP hjic-799 8 12 less less ADJ hjic-799 8 13 than than ADP hjic-799 8 14 5 5 NUM hjic-799 8 15 µl µl NOUN hjic-799 8 16 , , PUNCT hjic-799 8 17 dissolved dissolve VERB hjic-799 8 18 in in ADP hjic-799 8 19 water water NOUN hjic-799 8 20 and and CCONJ hjic-799 8 21 0.6 0.6 NUM hjic-799 8 22 µl µl ADP hjic-799 8 23 of of ADP hjic-799 8 24 5 5 NUM hjic-799 8 25 % % NOUN hjic-799 8 26 fa fa NOUN hjic-799 8 27 was be AUX hjic-799 8 28 used use VERB hjic-799 8 29 to to PART hjic-799 8 30 obtain obtain VERB hjic-799 8 31 15 15 NUM hjic-799 8 32 µl µl NOUN hjic-799 8 33 of of ADP hjic-799 8 34 the the DET hjic-799 8 35 peptide peptide NOUN hjic-799 8 36 solution solution NOUN hjic-799 8 37 before before ADP hjic-799 8 38 lc lc PROPN hjic-799 8 39 - - PUNCT hjic-799 8 40 ms ms PROPN hjic-799 8 41 / / SYM hjic-799 8 42 ms ms NOUN hjic-799 8 43 analysis analysis NOUN hjic-799 8 44 . . PUNCT hjic-799 9 1 2.3 2.3 NUM hjic-799 9 2 mass mass NOUN hjic-799 9 3 spectrometry spectrometry NOUN hjic-799 9 4 lc lc PROPN hjic-799 9 5 - - PUNCT hjic-799 9 6 ms ms PROPN hjic-799 9 7 / / SYM hjic-799 9 8 ms ms NOUN hjic-799 9 9 analyses analysis NOUN hjic-799 9 10 of of ADP hjic-799 9 11 the the DET hjic-799 9 12 peptide peptide NOUN hjic-799 9 13 mixture mixture NOUN hjic-799 9 14 were be AUX hjic-799 9 15 conducted conduct VERB hjic-799 9 16 using use VERB hjic-799 9 17 a a DET hjic-799 9 18 rslcnano rslcnano NOUN hjic-799 9 19 system system NOUN hjic-799 9 20 connected connect VERB hjic-799 9 21 to to ADP hjic-799 9 22 a a DET hjic-799 9 23 orbitrap orbitrap ADJ hjic-799 9 24 elite elite ADJ hjic-799 9 25 hybrid hybrid ADJ hjic-799 9 26 mass mass NOUN hjic-799 9 27 spectrometer spectrometer NOUN hjic-799 9 28 ( ( PUNCT hjic-799 9 29 thermo thermo NOUN hjic-799 9 30 fisher fisher PROPN hjic-799 9 31 scientific scientific PROPN hjic-799 9 32 ) ) PUNCT hjic-799 9 33 with with ADP hjic-799 9 34 a a DET hjic-799 9 35 abird abird NOUN hjic-799 9 36 ( ( PUNCT hjic-799 9 37 active active ADJ hjic-799 9 38 background background NOUN hjic-799 9 39 ion ion NOUN hjic-799 9 40 reduction reduction NOUN hjic-799 9 41 device device NOUN hjic-799 9 42 ; ; PUNCT hjic-799 9 43 esi esi X hjic-799 9 44 source source NOUN hjic-799 9 45 solutions solution NOUN hjic-799 9 46 ) ) PUNCT hjic-799 9 47 and and CCONJ hjic-799 9 48 a a DET hjic-799 9 49 digital digital ADJ hjic-799 9 50 picoview picoview NOUN hjic-799 9 51 dpv550 dpv550 PROPN hjic-799 9 52 ( ( PUNCT hjic-799 9 53 new new ADJ hjic-799 9 54 objective objective NOUN hjic-799 9 55 ) ) PUNCT hjic-799 9 56 ion ion NOUN hjic-799 9 57 source source NOUN hjic-799 9 58 ( ( PUNCT hjic-799 9 59 tip tip NOUN hjic-799 9 60 rinsing rinsing NOUN hjic-799 9 61 by by ADP hjic-799 9 62 50 50 NUM hjic-799 9 63 % % NOUN hjic-799 9 64 acn acn NOUN hjic-799 9 65 with with ADP hjic-799 9 66 0.1 0.1 NUM hjic-799 9 67 % % NOUN hjic-799 9 68 fa fa NOUN hjic-799 9 69 ) ) PUNCT hjic-799 9 70 installed instal VERB hjic-799 9 71 . . PUNCT hjic-799 10 1 prior prior ADV hjic-799 10 2 to to ADP hjic-799 10 3 lc lc PROPN hjic-799 10 4 separation separation NOUN hjic-799 10 5 , , PUNCT hjic-799 10 6 tryptic tryptic ADJ hjic-799 10 7 digests digest NOUN hjic-799 10 8 were be AUX hjic-799 10 9 concentrated concentrate VERB hjic-799 10 10 online online ADV hjic-799 10 11 and and CCONJ hjic-799 10 12 desalted desalt VERB hjic-799 10 13 using use VERB hjic-799 10 14 a a DET hjic-799 10 15 trapping trap VERB hjic-799 10 16 column column NOUN hjic-799 10 17 ( ( PUNCT hjic-799 10 18 100 100 NUM hjic-799 10 19 µm µm PART hjic-799 10 20 × × NOUN hjic-799 10 21 30 30 NUM hjic-799 10 22 mm mm NOUN hjic-799 10 23 ) ) PUNCT hjic-799 10 24 filled fill VERB hjic-799 10 25 with with ADP hjic-799 10 26 3.5-µm 3.5-µm NUM hjic-799 10 27 of of ADP hjic-799 10 28 xbridge xbridge PROPN hjic-799 10 29 beh beh PROPN hjic-799 10 30 130å 130å PROPN hjic-799 10 31 c18 c18 NOUN hjic-799 10 32 sorbent sorbent NOUN hjic-799 10 33 ( ( PUNCT hjic-799 10 34 waters water NOUN hjic-799 10 35 ) ) PUNCT hjic-799 10 36 . . PUNCT hjic-799 11 1 after after SCONJ hjic-799 11 2 the the DET hjic-799 11 3 trapping trap VERB hjic-799 11 4 column column NOUN hjic-799 11 5 was be AUX hjic-799 11 6 washed wash VERB hjic-799 11 7 with with ADP hjic-799 11 8 0.1 0.1 NUM hjic-799 11 9 % % NOUN hjic-799 11 10 fa fa NOUN hjic-799 11 11 , , PUNCT hjic-799 11 12 the the DET hjic-799 11 13 peptides peptide NOUN hjic-799 11 14 were be AUX hjic-799 11 15 eluted elute VERB hjic-799 11 16 ( ( PUNCT hjic-799 11 17 flow flow NOUN hjic-799 11 18 rate rate NOUN hjic-799 11 19 of of ADP hjic-799 11 20 300 300 NUM hjic-799 11 21 nl nl PROPN hjic-799 11 22 / / SYM hjic-799 11 23 min min NOUN hjic-799 11 24 ) ) PUNCT hjic-799 11 25 from from ADP hjic-799 11 26 the the DET hjic-799 11 27 trapping trap VERB hjic-799 11 28 column column NOUN hjic-799 11 29 onto onto ADP hjic-799 11 30 a a DET hjic-799 11 31 acclaim acclaim NOUN hjic-799 11 32 pepmap100 pepmap100 PROPN hjic-799 11 33 c18 c18 PROPN hjic-799 11 34 column column NOUN hjic-799 11 35 ( ( PUNCT hjic-799 11 36 3 3 NUM hjic-799 11 37 µm µm ADP hjic-799 11 38 particles particle NOUN hjic-799 11 39 , , PUNCT hjic-799 11 40 75 75 NUM hjic-799 11 41 µm µm PART hjic-799 11 42 × × PROPN hjic-799 11 43 500 500 NUM hjic-799 11 44 mm mm NOUN hjic-799 11 45 ; ; PUNCT hjic-799 11 46 thermo thermo PROPN hjic-799 11 47 fisher fisher PROPN hjic-799 11 48 scientific scientific PROPN hjic-799 11 49 ) ) PUNCT hjic-799 11 50 along along ADP hjic-799 11 51 a a DET hjic-799 11 52 65 65 NUM hjic-799 11 53 min min NOUN hjic-799 11 54 - - ADJ hjic-799 11 55 long long ADJ hjic-799 11 56 gradient gradient NOUN hjic-799 11 57 . . PUNCT hjic-799 12 1 mobile mobile ADJ hjic-799 12 2 phase phase NOUN hjic-799 12 3 a a DET hjic-799 12 4 ( ( PUNCT hjic-799 12 5 0.1 0.1 NUM hjic-799 12 6 % % NOUN hjic-799 12 7 fa fa NOUN hjic-799 12 8 in in ADP hjic-799 12 9 water water NOUN hjic-799 12 10 ) ) PUNCT hjic-799 12 11 and and CCONJ hjic-799 12 12 mobile mobile ADJ hjic-799 12 13 phase phase NOUN hjic-799 12 14 b b PROPN hjic-799 12 15 ( ( PUNCT hjic-799 12 16 0.1 0.1 NUM hjic-799 12 17 % % NOUN hjic-799 12 18 fa fa NOUN hjic-799 12 19 in in ADP hjic-799 12 20 80 80 NUM hjic-799 12 21 % % NOUN hjic-799 12 22 acn acn PROPN hjic-799 12 23 ) ) PUNCT hjic-799 12 24 were be AUX hjic-799 12 25 used use VERB hjic-799 12 26 in in ADP hjic-799 12 27 both both DET hjic-799 12 28 cases case NOUN hjic-799 12 29 . . PUNCT hjic-799 13 1 the the DET hjic-799 13 2 gradient gradient PROPN hjic-799 13 3 elution elution NOUN hjic-799 13 4 started start VERB hjic-799 13 5 at at ADP hjic-799 13 6 1 1 NUM hjic-799 13 7 % % NOUN hjic-799 13 8 of of ADP hjic-799 13 9 mobile mobile ADJ hjic-799 13 10 phase phase NOUN hjic-799 13 11 b b PROPN hjic-799 13 12 and and CCONJ hjic-799 13 13 increased increase VERB hjic-799 13 14 from from ADP hjic-799 13 15 1 1 NUM hjic-799 13 16 % % NOUN hjic-799 13 17 to to ADP hjic-799 13 18 56 56 NUM hjic-799 13 19 % % NOUN hjic-799 13 20 during during ADP hjic-799 13 21 the the DET hjic-799 13 22 first first ADJ hjic-799 13 23 50 50 NUM hjic-799 13 24 mins min NOUN hjic-799 13 25 ( ( PUNCT hjic-799 13 26 30 30 NUM hjic-799 13 27 % % NOUN hjic-799 13 28 in in ADP hjic-799 13 29 the the DET hjic-799 13 30 35th 35th ADJ hjic-799 13 31 and and CCONJ hjic-799 13 32 56 56 NUM hjic-799 13 33 % % NOUN hjic-799 13 34 in in ADP hjic-799 13 35 the the DET hjic-799 13 36 50th 50th ADJ hjic-799 13 37 min min NOUN hjic-799 13 38 ) ) PUNCT hjic-799 13 39 , , PUNCT hjic-799 13 40 then then ADV hjic-799 13 41 increased increase VERB hjic-799 13 42 linearly linearly ADV hjic-799 13 43 to to ADP hjic-799 13 44 80 80 NUM hjic-799 13 45 % % NOUN hjic-799 13 46 of of ADP hjic-799 13 47 mobile mobile ADJ hjic-799 13 48 phase phase NOUN hjic-799 13 49 b b NOUN hjic-799 13 50 over over ADP hjic-799 13 51 the the DET hjic-799 13 52 following follow VERB hjic-799 13 53 5 5 NUM hjic-799 13 54 mins min NOUN hjic-799 13 55 and and CCONJ hjic-799 13 56 remained remain VERB hjic-799 13 57 at at ADP hjic-799 13 58 this this DET hjic-799 13 59 state state NOUN hjic-799 13 60 for for ADP hjic-799 13 61 the the DET hjic-799 13 62 next next ADJ hjic-799 13 63 10 10 NUM hjic-799 13 64 mins min NOUN hjic-799 13 65 . . PUNCT hjic-799 14 1 equilibration equilibration NOUN hjic-799 14 2 of of ADP hjic-799 14 3 the the DET hjic-799 14 4 trapping trap VERB hjic-799 14 5 column column NOUN hjic-799 14 6 and and CCONJ hjic-799 14 7 the the DET hjic-799 14 8 anlytical anlytical ADJ hjic-799 14 9 column column NOUN hjic-799 14 10 was be AUX hjic-799 14 11 conducted conduct VERB hjic-799 14 12 prior prior ADV hjic-799 14 13 to to ADP hjic-799 14 14 injection injection NOUN hjic-799 14 15 of of ADP hjic-799 14 16 the the DET hjic-799 14 17 sample sample NOUN hjic-799 14 18 into into ADP hjic-799 14 19 the the DET hjic-799 14 20 sample sample NOUN hjic-799 14 21 loop loop NOUN hjic-799 14 22 . . PUNCT hjic-799 15 1 the the DET hjic-799 15 2 outlet outlet NOUN hjic-799 15 3 of of ADP hjic-799 15 4 the the DET hjic-799 15 5 analytical analytical ADJ hjic-799 15 6 column column NOUN hjic-799 15 7 was be AUX hjic-799 15 8 directly directly ADV hjic-799 15 9 connected connect VERB hjic-799 15 10 to to ADP hjic-799 15 11 the the DET hjic-799 15 12 digital digital ADJ hjic-799 15 13 picoview picoview VERB hjic-799 15 14 dpv550 dpv550 PROPN hjic-799 15 15 ion ion NOUN hjic-799 15 16 source source NOUN hjic-799 15 17 . . PUNCT hjic-799 16 1 ms ms PROPN hjic-799 16 2 data data PROPN hjic-799 16 3 were be AUX hjic-799 16 4 acquired acquire VERB hjic-799 16 5 in in ADP hjic-799 16 6 a a DET hjic-799 16 7 data data NOUN hjic-799 16 8 - - PUNCT hjic-799 16 9 dependent dependent ADJ hjic-799 16 10 strategy strategy NOUN hjic-799 16 11 by by ADP hjic-799 16 12 selecting select VERB hjic-799 16 13 the the DET hjic-799 16 14 top top ADJ hjic-799 16 15 6 6 NUM hjic-799 16 16 precursors precursor NOUN hjic-799 16 17 based base VERB hjic-799 16 18 on on ADP hjic-799 16 19 precursor precursor NOUN hjic-799 16 20 abundance abundance NOUN hjic-799 16 21 in in ADP hjic-799 16 22 the the DET hjic-799 16 23 survey survey NOUN hjic-799 16 24 scan scan NOUN hjic-799 16 25 ( ( PUNCT hjic-799 16 26 350 350 NUM hjic-799 16 27 - - SYM hjic-799 16 28 2000 2000 NUM hjic-799 16 29 m m PROPN hjic-799 16 30 / / SYM hjic-799 16 31 z z NOUN hjic-799 16 32 ) ) PUNCT hjic-799 16 33 . . PUNCT hjic-799 17 1 the the DET hjic-799 17 2 resolution resolution NOUN hjic-799 17 3 of of ADP hjic-799 17 4 the the DET hjic-799 17 5 survey survey NOUN hjic-799 17 6 scan scan NOUN hjic-799 17 7 was be AUX hjic-799 17 8 60,000 60,000 NUM hjic-799 17 9 ( ( PUNCT hjic-799 17 10 at at ADP hjic-799 17 11 400 400 NUM hjic-799 17 12 m m NOUN hjic-799 17 13 / / SYM hjic-799 17 14 z z NOUN hjic-799 17 15 ) ) PUNCT hjic-799 17 16 with with ADP hjic-799 17 17 a a DET hjic-799 17 18 target target NOUN hjic-799 17 19 value value NOUN hjic-799 17 20 of of ADP hjic-799 17 21 1×106 1×106 NUM hjic-799 17 22 ions ion NOUN hjic-799 17 23 , , PUNCT hjic-799 17 24 one one NUM hjic-799 17 25 microscan microscan NOUN hjic-799 17 26 and and CCONJ hjic-799 17 27 a a DET hjic-799 17 28 maximum maximum ADJ hjic-799 17 29 injection injection NOUN hjic-799 17 30 time time NOUN hjic-799 17 31 of of ADP hjic-799 17 32 200 200 NUM hjic-799 17 33 ms ms PROPN hjic-799 17 34 . . PROPN hjic-799 17 35 high high ADJ hjic-799 17 36 resolution resolution NOUN hjic-799 17 37 ( ( PUNCT hjic-799 17 38 resolution resolution NOUN hjic-799 17 39 of of ADP hjic-799 17 40 15,000 15,000 NUM hjic-799 17 41 at at ADP hjic-799 17 42 400 400 NUM hjic-799 17 43 m m PROPN hjic-799 17 44 / / SYM hjic-799 17 45 z z NOUN hjic-799 17 46 ) ) PUNCT hjic-799 17 47 higher high ADJ hjic-799 17 48 energy energy NOUN hjic-799 17 49 collisional collisional ADJ hjic-799 17 50 dissociation dissociation NOUN hjic-799 17 51 ( ( PUNCT hjic-799 17 52 hcd hcd ADJ hjic-799 17 53 ) ) PUNCT hjic-799 17 54 ms ms PROPN hjic-799 17 55 / / SYM hjic-799 17 56 ms ms PROPN hjic-799 17 57 spectra spectra PROPN hjic-799 17 58 were be AUX hjic-799 17 59 acquired acquire VERB hjic-799 17 60 with with ADP hjic-799 17 61 a a DET hjic-799 17 62 target target NOUN hjic-799 17 63 value value NOUN hjic-799 17 64 of of ADP hjic-799 17 65 50,000 50,000 NUM hjic-799 17 66 . . PUNCT hjic-799 18 1 the the DET hjic-799 18 2 normalized normalize VERB hjic-799 18 3 collision collision NOUN hjic-799 18 4 energy energy NOUN hjic-799 18 5 was be AUX hjic-799 18 6 32 32 NUM hjic-799 18 7 % % NOUN hjic-799 18 8 for for ADP hjic-799 18 9 hcd hcd ADJ hjic-799 18 10 spectra spectra PROPN hjic-799 18 11 . . PUNCT hjic-799 19 1 the the DET hjic-799 19 2 maximum maximum ADJ hjic-799 19 3 injection injection NOUN hjic-799 19 4 time time NOUN hjic-799 19 5 for for SCONJ hjic-799 19 6 ms ms PROPN hjic-799 19 7 / / SYM hjic-799 19 8 ms ms PROPN hjic-799 19 9 was be AUX hjic-799 19 10 500 500 NUM hjic-799 19 11 ms ms PROPN hjic-799 19 12 . . PROPN hjic-799 19 13 dynamic dynamic ADJ hjic-799 19 14 exclusion exclusion NOUN hjic-799 19 15 was be AUX hjic-799 19 16 enabled enable VERB hjic-799 19 17 for for ADP hjic-799 19 18 45 45 NUM hjic-799 19 19 s s NOUN hjic-799 19 20 after after SCONJ hjic-799 19 21 the the DET hjic-799 19 22 acquisition acquisition NOUN hjic-799 19 23 of of ADP hjic-799 19 24 one one NUM hjic-799 19 25 ms ms PROPN hjic-799 19 26 / / SYM hjic-799 19 27 ms ms PROPN hjic-799 19 28 spectra spectra NOUN hjic-799 19 29 and and CCONJ hjic-799 19 30 early early ADJ hjic-799 19 31 expiration expiration NOUN hjic-799 19 32 was be AUX hjic-799 19 33 disabled disable VERB hjic-799 19 34 . . PUNCT hjic-799 20 1 the the DET hjic-799 20 2 isolation isolation NOUN hjic-799 20 3 window window NOUN hjic-799 20 4 for for ADP hjic-799 20 5 ms ms PROPN hjic-799 20 6 / / SYM hjic-799 20 7 ms ms PROPN hjic-799 20 8 fragmentation fragmentation NOUN hjic-799 20 9 was be AUX hjic-799 20 10 set set VERB hjic-799 20 11 to to ADP hjic-799 20 12 2 2 NUM hjic-799 20 13 m m NOUN hjic-799 20 14 / / SYM hjic-799 20 15 z. z. PROPN hjic-799 21 1 the the DET hjic-799 21 2 analysis analysis NOUN hjic-799 21 3 of of ADP hjic-799 21 4 the the DET hjic-799 21 5 mass mass ADJ hjic-799 21 6 spectrometric spectrometric ADJ hjic-799 21 7 raw raw ADJ hjic-799 21 8 data data NOUN hjic-799 21 9 files file NOUN hjic-799 21 10 was be AUX hjic-799 21 11 carried carry VERB hjic-799 21 12 out out ADP hjic-799 21 13 using use VERB hjic-799 21 14 the the DET hjic-799 21 15 proteome proteome PROPN hjic-799 21 16 discoverer discoverer PROPN hjic-799 21 17 software software NOUN hjic-799 21 18 ( ( PUNCT hjic-799 21 19 thermo thermo NOUN hjic-799 21 20 fisher fisher PROPN hjic-799 21 21 scientific scientific PROPN hjic-799 21 22 ; ; PUNCT hjic-799 21 23 version version NOUN hjic-799 21 24 1.4 1.4 NUM hjic-799 21 25 ) ) PUNCT hjic-799 21 26 with with ADP hjic-799 21 27 utilization utilization NOUN hjic-799 21 28 of of ADP hjic-799 21 29 the the DET hjic-799 21 30 in in ADP hjic-799 21 31 - - PUNCT hjic-799 21 32 house house NOUN hjic-799 21 33 mascot mascot NOUN hjic-799 21 34 ( ( PUNCT hjic-799 21 35 matrixscience matrixscience NOUN hjic-799 21 36 ; ; PUNCT hjic-799 21 37 version version NOUN hjic-799 21 38 2.4.1 2.4.1 NUM hjic-799 21 39 ) ) PUNCT hjic-799 21 40 search search NOUN hjic-799 21 41 engine engine NOUN hjic-799 21 42 . . PUNCT hjic-799 22 1 ms ms PROPN hjic-799 22 2 / / SYM hjic-799 22 3 ms ms PROPN hjic-799 22 4 ion ion NOUN hjic-799 22 5 searches search NOUN hjic-799 22 6 were be AUX hjic-799 22 7 conducted conduct VERB hjic-799 22 8 against against ADP hjic-799 22 9 an an DET hjic-799 22 10 in in ADP hjic-799 22 11 - - PUNCT hjic-799 22 12 house house NOUN hjic-799 22 13 database database NOUN hjic-799 22 14 containing contain VERB hjic-799 22 15 the the DET hjic-799 22 16 expected expect VERB hjic-799 22 17 protein protein NOUN hjic-799 22 18 of of ADP hjic-799 22 19 interest interest NOUN hjic-799 22 20 with with ADP hjic-799 22 21 additional additional ADJ hjic-799 22 22 sequences sequence NOUN hjic-799 22 23 from from ADP hjic-799 22 24 the the DET hjic-799 22 25 crap crap NOUN hjic-799 22 26 ( ( PUNCT hjic-799 22 27 common common ADJ hjic-799 22 28 repository repository NOUN hjic-799 22 29 of of ADP hjic-799 22 30 adventitious adventitious ADJ hjic-799 22 31 proteins protein NOUN hjic-799 22 32 ) ) PUNCT hjic-799 22 33 database database NOUN hjic-799 22 34 ( ( PUNCT hjic-799 22 35 downloaded download VERB hjic-799 22 36 from from ADP hjic-799 22 37 http://www.thegpm.org/crap/ http://www.thegpm.org/crap/ NOUN hjic-799 22 38 ) ) PUNCT hjic-799 22 39 . . PUNCT hjic-799 23 1 mass mass ADJ hjic-799 23 2 tolerance tolerance NOUN hjic-799 23 3 for for ADP hjic-799 23 4 peptides peptide NOUN hjic-799 23 5 and and CCONJ hjic-799 23 6 ms ms PROPN hjic-799 23 7 / / SYM hjic-799 23 8 ms ms PROPN hjic-799 23 9 fragments fragment NOUN hjic-799 23 10 were be AUX hjic-799 23 11 7 7 NUM hjic-799 23 12 ppm ppm NOUN hjic-799 23 13 and and CCONJ hjic-799 23 14 0.03 0.03 NUM hjic-799 23 15 da da NOUN hjic-799 23 16 , , PUNCT hjic-799 23 17 respectively respectively ADV hjic-799 23 18 . . PUNCT hjic-799 24 1 oxidation oxidation NOUN hjic-799 24 2 of of ADP hjic-799 24 3 methionine methionine NOUN hjic-799 24 4 , , PUNCT hjic-799 24 5 deamidation deamidation NOUN hjic-799 24 6 ( ( PUNCT hjic-799 24 7 n n CCONJ hjic-799 24 8 , , PUNCT hjic-799 24 9 q q ADJ hjic-799 24 10 ) ) PUNCT hjic-799 24 11 hungarian hungarian ADJ hjic-799 24 12 journal journal NOUN hjic-799 24 13 of of ADP hjic-799 24 14 industry industry NOUN hjic-799 24 15 and and CCONJ hjic-799 24 16 chemistry chemistry NOUN hjic-799 24 17 semiquantitative semiquantitative ADJ hjic-799 24 18 analysis analysis NOUN hjic-799 24 19 of of ADP hjic-799 24 20 dvl3 dvl3 NOUN hjic-799 24 21 phosphorylations phosphorylation NOUN hjic-799 24 22 5 5 NUM hjic-799 24 23 figure figure NOUN hjic-799 24 24 2 2 NUM hjic-799 24 25 : : PUNCT hjic-799 24 26 semiquantitative semiquantitative ADJ hjic-799 24 27 analysis analysis NOUN hjic-799 24 28 of of ADP hjic-799 24 29 phosphorylation phosphorylation NOUN hjic-799 24 30 site site NOUN hjic-799 24 31 serine serine NOUN hjic-799 24 32 204 204 NUM hjic-799 24 33 ( ( PUNCT hjic-799 24 34 s204 s204 NUM hjic-799 24 35 ) ) PUNCT hjic-799 24 36 on on ADP hjic-799 24 37 peptide peptide ADJ hjic-799 24 38 fsspsteqssasr fsspsteqssasr PROPN hjic-799 24 39 induced induce VERB hjic-799 24 40 by by ADP hjic-799 24 41 ck1ε ck1ε PROPN hjic-799 24 42 and and CCONJ hjic-799 24 43 nek2 nek2 PROPN hjic-799 24 44 . . PUNCT hjic-799 25 1 precursor precursor NOUN hjic-799 25 2 and and CCONJ hjic-799 25 3 selected select VERB hjic-799 25 4 fragment fragment ADJ hjic-799 25 5 traces trace NOUN hjic-799 25 6 of of ADP hjic-799 25 7 corresponding correspond VERB hjic-799 25 8 hdvl3 hdvl3 NOUN hjic-799 25 9 phosphopeptides phosphopeptide NOUN hjic-799 25 10 are be AUX hjic-799 25 11 shown show VERB hjic-799 25 12 for for ADP hjic-799 25 13 ck1ε ck1ε PROPN hjic-799 25 14 and and CCONJ hjic-799 25 15 nek2 nek2 PROPN hjic-799 25 16 ( ( PUNCT hjic-799 25 17 in in ADP hjic-799 25 18 skyline skyline NOUN hjic-799 25 19 ) ) PUNCT hjic-799 25 20 . . PUNCT hjic-799 26 1 the the DET hjic-799 26 2 highest high ADJ hjic-799 26 3 signal signal ADJ hjic-799 26 4 intensity intensity NOUN hjic-799 26 5 was be AUX hjic-799 26 6 detected detect VERB hjic-799 26 7 in in ADP hjic-799 26 8 the the DET hjic-799 26 9 case case NOUN hjic-799 26 10 of of ADP hjic-799 26 11 nek2 nek2 PROPN hjic-799 26 12 . . PUNCT hjic-799 27 1 and and CCONJ hjic-799 27 2 phosphorylation phosphorylation NOUN hjic-799 27 3 ( ( PUNCT hjic-799 27 4 s s PROPN hjic-799 27 5 , , PUNCT hjic-799 27 6 t t PROPN hjic-799 27 7 , , PUNCT hjic-799 27 8 y y PROPN hjic-799 27 9 ) ) PUNCT hjic-799 27 10 as as ADP hjic-799 27 11 optional optional ADJ hjic-799 27 12 modifications modification NOUN hjic-799 27 13 , , PUNCT hjic-799 27 14 carbamidomethylation carbamidomethylation NOUN hjic-799 27 15 of of ADP hjic-799 27 16 c c PROPN hjic-799 27 17 as as ADP hjic-799 27 18 a a DET hjic-799 27 19 fixed fix VERB hjic-799 27 20 modification modification NOUN hjic-799 27 21 and and CCONJ hjic-799 27 22 three three NUM hjic-799 27 23 miss miss ADJ hjic-799 27 24 cleavages cleavage NOUN hjic-799 27 25 of of ADP hjic-799 27 26 enzymes enzyme NOUN hjic-799 27 27 were be AUX hjic-799 27 28 set set VERB hjic-799 27 29 for for ADP hjic-799 27 30 all all DET hjic-799 27 31 searches search NOUN hjic-799 27 32 . . PUNCT hjic-799 28 1 the the DET hjic-799 28 2 phosphors phosphor NOUN hjic-799 28 3 feature feature NOUN hjic-799 28 4 was be AUX hjic-799 28 5 used use VERB hjic-799 28 6 for for ADP hjic-799 28 7 phosphorylation phosphorylation NOUN hjic-799 28 8 site site NOUN hjic-799 28 9 localization localization NOUN hjic-799 28 10 . . PUNCT hjic-799 29 1 quantitative quantitative ADJ hjic-799 29 2 information information NOUN hjic-799 29 3 was be AUX hjic-799 29 4 assessed assess VERB hjic-799 29 5 and and CCONJ hjic-799 29 6 manually manually ADV hjic-799 29 7 validated validate VERB hjic-799 29 8 in in ADP hjic-799 29 9 skyline skyline ADJ hjic-799 29 10 software software NOUN hjic-799 29 11 ( ( PUNCT hjic-799 29 12 skyline skyline NOUN hjic-799 29 13 - - PUNCT hjic-799 29 14 daily daily ADJ hjic-799 29 15 3.1.1.8884 3.1.1.8884 NUM hjic-799 29 16 ) ) PUNCT hjic-799 29 17 . . PUNCT hjic-799 30 1 3 3 X hjic-799 30 2 . . NOUN hjic-799 30 3 results result NOUN hjic-799 30 4 and and CCONJ hjic-799 30 5 analysis analysis NOUN hjic-799 30 6 3.1 3.1 NUM hjic-799 30 7 identification identification NOUN hjic-799 30 8 of of ADP hjic-799 30 9 phosphorylation phosphorylation NOUN hjic-799 30 10 sites site NOUN hjic-799 30 11 phosphorylation phosphorylation NOUN hjic-799 30 12 is be AUX hjic-799 30 13 important important ADJ hjic-799 30 14 for for ADP hjic-799 30 15 protein protein NOUN hjic-799 30 16 function function NOUN hjic-799 30 17 and and CCONJ hjic-799 30 18 regulation regulation NOUN hjic-799 30 19 . . PUNCT hjic-799 31 1 the the DET hjic-799 31 2 phosphorylation phosphorylation NOUN hjic-799 31 3 status status NOUN hjic-799 31 4 of of ADP hjic-799 31 5 human human ADJ hjic-799 31 6 dvl3 dvl3 NOUN hjic-799 31 7 induced induce VERB hjic-799 31 8 by by ADP hjic-799 31 9 eight eight NUM hjic-799 31 10 individual individual ADJ hjic-799 31 11 ser ser NOUN hjic-799 31 12 / / SYM hjic-799 31 13 thr thr PROPN hjic-799 31 14 kinases kinase NOUN hjic-799 31 15 that that PRON hjic-799 31 16 were be AUX hjic-799 31 17 previously previously ADV hjic-799 31 18 reported report VERB hjic-799 31 19 or or CCONJ hjic-799 31 20 identified identify VERB hjic-799 31 21 by by ADP hjic-799 31 22 an an DET hjic-799 31 23 unbiased unbiased ADJ hjic-799 31 24 ms ms NOUN hjic-799 31 25 screen screen NOUN hjic-799 31 26 for for ADP hjic-799 31 27 dvl dvl PROPN hjic-799 31 28 - - PUNCT hjic-799 31 29 associated associate VERB hjic-799 31 30 kinases kinase NOUN hjic-799 31 31 was be AUX hjic-799 31 32 analysed analyse VERB hjic-799 31 33 . . PUNCT hjic-799 32 1 dvl3 dvl3 NOUN hjic-799 32 2 contains contain VERB hjic-799 32 3 131 131 NUM hjic-799 32 4 serines serine NOUN hjic-799 32 5 / / SYM hjic-799 32 6 threonines threonine NOUN hjic-799 32 7 , , PUNCT hjic-799 32 8 which which PRON hjic-799 32 9 can can AUX hjic-799 32 10 be be AUX hjic-799 32 11 potentially potentially ADV hjic-799 32 12 phosphorylated phosphorylate VERB hjic-799 32 13 . . PUNCT hjic-799 33 1 in in ADP hjic-799 33 2 total total ADJ hjic-799 33 3 , , PUNCT hjic-799 33 4 88 88 NUM hjic-799 33 5 ser ser NOUN hjic-799 33 6 / / SYM hjic-799 33 7 thr thr PROPN hjic-799 33 8 phosphorylation phosphorylation NOUN hjic-799 33 9 sites site NOUN hjic-799 33 10 and and CCONJ hjic-799 33 11 one one NUM hjic-799 33 12 tyrosine tyrosine NOUN hjic-799 33 13 phosphorylation phosphorylation NOUN hjic-799 33 14 site site NOUN hjic-799 33 15 in in ADP hjic-799 33 16 dvl3 dvl3 PROPN hjic-799 33 17 were be AUX hjic-799 33 18 identified identify VERB hjic-799 33 19 . . PUNCT hjic-799 34 1 3.2 3.2 NUM hjic-799 34 2 phosphorylations phosphorylation NOUN hjic-799 34 3 induced induce VERB hjic-799 34 4 by by ADP hjic-799 34 5 ck1ε ck1ε PROPN hjic-799 34 6 and and CCONJ hjic-799 34 7 nek2 nek2 PROPN hjic-799 34 8 based base VERB hjic-799 34 9 on on ADP hjic-799 34 10 our our PRON hjic-799 34 11 experiment experiment NOUN hjic-799 34 12 , , PUNCT hjic-799 34 13 a a DET hjic-799 34 14 phosphorylation phosphorylation NOUN hjic-799 34 15 “ " PUNCT hjic-799 34 16 map map NOUN hjic-799 34 17 ” " PUNCT hjic-799 34 18 of of ADP hjic-799 34 19 the the DET hjic-799 34 20 dvl dvl PROPN hjic-799 34 21 protein protein NOUN hjic-799 34 22 was be AUX hjic-799 34 23 created create VERB hjic-799 34 24 that that PRON hjic-799 34 25 described describe VERB hjic-799 34 26 the the DET hjic-799 34 27 complex complex ADJ hjic-799 34 28 phosphorylation phosphorylation NOUN hjic-799 34 29 “ " PUNCT hjic-799 34 30 fingerprint fingerprint NOUN hjic-799 34 31 ” " PUNCT hjic-799 34 32 for for ADP hjic-799 34 33 each each DET hjic-799 34 34 kinase kinase NOUN hjic-799 34 35 tested test VERB hjic-799 34 36 . . PUNCT hjic-799 35 1 eight eight NUM hjic-799 35 2 of of ADP hjic-799 35 3 the the DET hjic-799 35 4 kinases kinase NOUN hjic-799 35 5 used use VERB hjic-799 35 6 to to PART hjic-799 35 7 induce induce VERB hjic-799 35 8 phosphorylation phosphorylation NOUN hjic-799 35 9 include include VERB hjic-799 35 10 ck1ε ck1ε PROPN hjic-799 35 11 and and CCONJ hjic-799 35 12 nek2 nek2 PROPN hjic-799 35 13 . . PUNCT hjic-799 36 1 fig fig NOUN hjic-799 36 2 . . PUNCT hjic-799 37 1 1 1 NUM hjic-799 37 2 shows show VERB hjic-799 37 3 a a DET hjic-799 37 4 qualitative qualitative ADJ hjic-799 37 5 comparison comparison NOUN hjic-799 37 6 of of ADP hjic-799 37 7 the the DET hjic-799 37 8 identified identify VERB hjic-799 37 9 phosphorylation phosphorylation NOUN hjic-799 37 10 sites site NOUN hjic-799 37 11 using use VERB hjic-799 37 12 these these DET hjic-799 37 13 two two NUM hjic-799 37 14 kinases kinase NOUN hjic-799 37 15 . . PUNCT hjic-799 38 1 in in ADP hjic-799 38 2 the the DET hjic-799 38 3 case case NOUN hjic-799 38 4 of of ADP hjic-799 38 5 ck1ε ck1ε PROPN hjic-799 38 6 induction induction NOUN hjic-799 38 7 , , PUNCT hjic-799 38 8 77 77 NUM hjic-799 38 9 phosphorylation phosphorylation NOUN hjic-799 38 10 sites site NOUN hjic-799 38 11 were be AUX hjic-799 38 12 identified identify VERB hjic-799 38 13 , , PUNCT hjic-799 38 14 and and CCONJ hjic-799 38 15 in in ADP hjic-799 38 16 the the DET hjic-799 38 17 case case NOUN hjic-799 38 18 of of ADP hjic-799 38 19 nek2 nek2 NOUN hjic-799 38 20 , , PUNCT hjic-799 38 21 87 87 NUM hjic-799 38 22 phosphorylation phosphorylation NOUN hjic-799 38 23 sites site NOUN hjic-799 38 24 were be AUX hjic-799 38 25 determined determine VERB hjic-799 38 26 from from ADP hjic-799 38 27 a a DET hjic-799 38 28 total total NOUN hjic-799 38 29 of of ADP hjic-799 38 30 131 131 NUM hjic-799 38 31 possible possible ADJ hjic-799 38 32 ser ser NOUN hjic-799 38 33 / / SYM hjic-799 38 34 thr thr PROPN hjic-799 38 35 phosphorylation phosphorylation NOUN hjic-799 38 36 sites site NOUN hjic-799 38 37 in in ADP hjic-799 38 38 the the DET hjic-799 38 39 dvl3 dvl3 NOUN hjic-799 38 40 protein protein NOUN hjic-799 38 41 . . PUNCT hjic-799 39 1 next next ADJ hjic-799 39 2 in in ADP hjic-799 39 3 terms term NOUN hjic-799 39 4 of of ADP hjic-799 39 5 qualitative qualitative ADJ hjic-799 39 6 characterization characterization NOUN hjic-799 39 7 , , PUNCT hjic-799 39 8 a a DET hjic-799 39 9 semiquantitative semiquantitative ADJ hjic-799 39 10 comparison comparison NOUN hjic-799 39 11 with with ADP hjic-799 39 12 regard regard NOUN hjic-799 39 13 to to ADP hjic-799 39 14 the the DET hjic-799 39 15 occupancy occupancy NOUN hjic-799 39 16 of of ADP hjic-799 39 17 phosphorylation phosphorylation NOUN hjic-799 39 18 sites site NOUN hjic-799 39 19 induced induce VERB hjic-799 39 20 by by ADP hjic-799 39 21 individual individual ADJ hjic-799 39 22 kinases kinase NOUN hjic-799 39 23 was be AUX hjic-799 39 24 conducted conduct VERB hjic-799 39 25 . . PUNCT hjic-799 40 1 the the DET hjic-799 40 2 skyline skyline ADJ hjic-799 40 3 software software NOUN hjic-799 40 4 was be AUX hjic-799 40 5 used use VERB hjic-799 40 6 for for ADP hjic-799 40 7 this this DET hjic-799 40 8 evaluation evaluation NOUN hjic-799 40 9 . . PUNCT hjic-799 41 1 the the DET hjic-799 41 2 individual individual ADJ hjic-799 41 3 phosphorylated phosphorylate VERB hjic-799 41 4 peptides peptide NOUN hjic-799 41 5 were be AUX hjic-799 41 6 compared compare VERB hjic-799 41 7 based base VERB hjic-799 41 8 on on ADP hjic-799 41 9 their their PRON hjic-799 41 10 peak peak NOUN hjic-799 41 11 areas area NOUN hjic-799 41 12 . . PUNCT hjic-799 42 1 a a DET hjic-799 42 2 comparison comparison NOUN hjic-799 42 3 of of ADP hjic-799 42 4 a a DET hjic-799 42 5 selected select VERB hjic-799 42 6 peptide peptide NOUN hjic-799 42 7 phosphorylated phosphorylate VERB hjic-799 42 8 in in ADP hjic-799 42 9 the the DET hjic-799 42 10 position position NOUN hjic-799 42 11 of of ADP hjic-799 42 12 s204 s204 PROPN hjic-799 42 13 by by ADP hjic-799 42 14 ck1ε ck1ε PROPN hjic-799 42 15 and and CCONJ hjic-799 42 16 nek2 nek2 NOUN hjic-799 42 17 is be AUX hjic-799 42 18 shown show VERB hjic-799 42 19 in in ADP hjic-799 42 20 fig fig NOUN hjic-799 42 21 . . PUNCT hjic-799 43 1 2 2 X hjic-799 43 2 . . X hjic-799 43 3 the the DET hjic-799 43 4 peak peak NOUN hjic-799 43 5 area area NOUN hjic-799 43 6 was be AUX hjic-799 43 7 determined determine VERB hjic-799 43 8 for for ADP hjic-799 43 9 ck1ε ck1ε PROPN hjic-799 43 10 as as ADP hjic-799 43 11 7.60e6 7.60e6 NUM hjic-799 43 12 and and CCONJ hjic-799 43 13 for for ADP hjic-799 43 14 nek2 nek2 NOUN hjic-799 43 15 as as ADP hjic-799 43 16 1.07e9 1.07e9 NUM hjic-799 43 17 . . PUNCT hjic-799 44 1 subsequently subsequently ADV hjic-799 44 2 , , PUNCT hjic-799 44 3 double double ADJ hjic-799 44 4 normalization normalization NOUN hjic-799 44 5 of of ADP hjic-799 44 6 the the DET hjic-799 44 7 data datum NOUN hjic-799 44 8 was be AUX hjic-799 44 9 performed perform VERB hjic-799 44 10 using use VERB hjic-799 44 11 a a DET hjic-799 44 12 set set NOUN hjic-799 44 13 of of ADP hjic-799 44 14 phosphopeptide phosphopeptide NOUN hjic-799 44 15 standards standard NOUN hjic-799 44 16 ( ( PUNCT hjic-799 44 17 added add VERB hjic-799 44 18 to to ADP hjic-799 44 19 the the DET hjic-799 44 20 sample sample NOUN hjic-799 44 21 prior prior ADV hjic-799 44 22 to to ADP hjic-799 44 23 the the DET hjic-799 44 24 phospho phospho ADJ hjic-799 44 25 - - PUNCT hjic-799 44 26 enrichment enrichment NOUN hjic-799 44 27 step step NOUN hjic-799 44 28 ) ) PUNCT hjic-799 44 29 and and CCONJ hjic-799 44 30 by by ADP hjic-799 44 31 unphosphorylated unphosphorylated ADJ hjic-799 44 32 peptides peptide NOUN hjic-799 44 33 identified identify VERB hjic-799 44 34 in in ADP hjic-799 44 35 the the DET hjic-799 44 36 identification identification NOUN hjic-799 44 37 run run NOUN hjic-799 44 38 . . PUNCT hjic-799 45 1 the the DET hjic-799 45 2 resulting result VERB hjic-799 45 3 areas area NOUN hjic-799 45 4 ( ( PUNCT hjic-799 45 5 ck1ε ck1ε NOUN hjic-799 45 6 : : PUNCT hjic-799 45 7 1.23e7 1.23e7 NUM hjic-799 45 8 and and CCONJ hjic-799 45 9 nek2 nek2 PROPN hjic-799 45 10 : : PUNCT hjic-799 45 11 1.10e9 1.10e9 NUM hjic-799 45 12 ) ) PUNCT hjic-799 45 13 were be AUX hjic-799 45 14 compared compare VERB hjic-799 45 15 with with ADP hjic-799 45 16 each each DET hjic-799 45 17 other other ADJ hjic-799 45 18 . . PUNCT hjic-799 46 1 4 4 X hjic-799 46 2 . . X hjic-799 46 3 discussion discussion NOUN hjic-799 46 4 our our PRON hjic-799 46 5 study study NOUN hjic-799 46 6 focused focus VERB hjic-799 46 7 on on ADP hjic-799 46 8 the the DET hjic-799 46 9 determination determination NOUN hjic-799 46 10 of of ADP hjic-799 46 11 the the DET hjic-799 46 12 phosphorylation phosphorylation NOUN hjic-799 46 13 sites site NOUN hjic-799 46 14 of of ADP hjic-799 46 15 dvl3 dvl3 NOUN hjic-799 46 16 by by ADP hjic-799 46 17 ms ms PROPN hjic-799 46 18 induced induce VERB hjic-799 46 19 by by ADP hjic-799 46 20 eight eight NUM hjic-799 46 21 kinases kinase NOUN hjic-799 46 22 . . PUNCT hjic-799 47 1 88 88 NUM hjic-799 47 2 ser ser NOUN hjic-799 47 3 / / SYM hjic-799 47 4 thr thr NOUN hjic-799 47 5 phosphorylations phosphorylation NOUN hjic-799 47 6 from from ADP hjic-799 47 7 a a DET hjic-799 47 8 total total NOUN hjic-799 47 9 of of ADP hjic-799 47 10 131 131 NUM hjic-799 47 11 sites site NOUN hjic-799 47 12 and and CCONJ hjic-799 47 13 1 1 NUM hjic-799 47 14 tyrosine tyrosine NOUN hjic-799 47 15 phosphorylation phosphorylation NOUN hjic-799 47 16 were be AUX hjic-799 47 17 identified identify VERB hjic-799 47 18 which which PRON hjic-799 47 19 can can AUX hjic-799 47 20 be be AUX hjic-799 47 21 potentially potentially ADV hjic-799 47 22 phosphorylated phosphorylate VERB hjic-799 47 23 . . PUNCT hjic-799 48 1 ck1ε ck1ε NOUN hjic-799 48 2 - - PUNCT hjic-799 48 3 induced induce VERB hjic-799 48 4 phosphorylation phosphorylation NOUN hjic-799 48 5 was be AUX hjic-799 48 6 identified identify VERB hjic-799 48 7 at at ADP hjic-799 48 8 77 77 NUM hjic-799 48 9 unique unique ADJ hjic-799 48 10 sites site NOUN hjic-799 48 11 and and CCONJ hjic-799 48 12 10 10 NUM hjic-799 48 13 more more ADJ hjic-799 48 14 phosphorylation phosphorylation NOUN hjic-799 48 15 sites site NOUN hjic-799 48 16 were be AUX hjic-799 48 17 induced induce VERB hjic-799 48 18 by by ADP hjic-799 48 19 nek2 nek2 PROPN hjic-799 48 20 . . PUNCT hjic-799 49 1 previous previous ADJ hjic-799 49 2 studies study NOUN hjic-799 49 3 in in ADP hjic-799 49 4 various various ADJ hjic-799 49 5 experimental experimental ADJ hjic-799 49 6 systems system NOUN hjic-799 49 7 identified identify VERB hjic-799 49 8 several several ADJ hjic-799 49 9 phosphorylation phosphorylation NOUN hjic-799 49 10 sites site NOUN hjic-799 49 11 spread spread VERB hjic-799 49 12 throughout throughout ADP hjic-799 49 13 the the DET hjic-799 49 14 structure structure NOUN hjic-799 49 15 of of ADP hjic-799 49 16 the the DET hjic-799 49 17 protein protein NOUN hjic-799 49 18 dvl3 dvl3 NOUN hjic-799 50 1 [ [ X hjic-799 50 2 3 3 NUM hjic-799 50 3 , , PUNCT hjic-799 50 4 4 4 NUM hjic-799 50 5 ] ] PUNCT hjic-799 50 6 . . PUNCT hjic-799 51 1 our our PRON hjic-799 51 2 data datum NOUN hjic-799 51 3 clearly clearly ADV hjic-799 51 4 demonstrate demonstrate VERB hjic-799 51 5 that that SCONJ hjic-799 51 6 the the DET hjic-799 51 7 phosphorylation phosphorylation NOUN hjic-799 51 8 of of ADP hjic-799 51 9 the the DET hjic-799 51 10 protein protein NOUN hjic-799 51 11 dvl3 dvl3 NOUN hjic-799 51 12 is be AUX hjic-799 51 13 extensive extensive ADJ hjic-799 51 14 and and CCONJ hjic-799 51 15 the the DET hjic-799 51 16 number number NOUN hjic-799 51 17 of of ADP hjic-799 51 18 phosphorylated phosphorylate VERB hjic-799 51 19 sites site NOUN hjic-799 51 20 exceeds exceed VERB hjic-799 51 21 60 60 NUM hjic-799 51 22 . . PUNCT hjic-799 52 1 5 5 NUM hjic-799 52 2 . . X hjic-799 52 3 conclusion conclusion NOUN hjic-799 52 4 an an DET hjic-799 52 5 approach approach NOUN hjic-799 52 6 based base VERB hjic-799 52 7 on on ADP hjic-799 52 8 the the DET hjic-799 52 9 sds sds NOUN hjic-799 52 10 - - PUNCT hjic-799 52 11 page page NOUN hjic-799 52 12 separation separation NOUN hjic-799 52 13 of of ADP hjic-799 52 14 dvl3 dvl3 NOUN hjic-799 52 15 immunoprecipitates immunoprecipitate NOUN hjic-799 52 16 , , PUNCT hjic-799 52 17 tio2 tio2 NOUN hjic-799 52 18 phospho phospho NOUN hjic-799 52 19 - - PUNCT hjic-799 52 20 enrichment enrichment NOUN hjic-799 52 21 followed follow VERB hjic-799 52 22 by by ADP hjic-799 52 23 lc lc PROPN hjic-799 52 24 - - PUNCT hjic-799 52 25 ms ms PROPN hjic-799 52 26 / / SYM hjic-799 52 27 ms ms NOUN hjic-799 52 28 analysis analysis NOUN hjic-799 52 29 and and CCONJ hjic-799 52 30 data datum NOUN hjic-799 52 31 processing processing NOUN hjic-799 52 32 using use VERB hjic-799 52 33 skyline skyline ADJ hjic-799 52 34 software software NOUN hjic-799 52 35 was be AUX hjic-799 52 36 utilized utilize VERB hjic-799 52 37 for for ADP hjic-799 52 38 the the DET hjic-799 52 39 evaluation evaluation NOUN hjic-799 52 40 of of ADP hjic-799 52 41 semiquantitative semiquantitative ADJ hjic-799 52 42 differences difference NOUN hjic-799 52 43 in in ADP hjic-799 52 44 the the DET hjic-799 52 45 phosphorylation phosphorylation NOUN hjic-799 52 46 level level NOUN hjic-799 52 47 of of ADP hjic-799 52 48 hdvl3 hdvl3 NOUN hjic-799 52 49 at at ADP hjic-799 52 50 particular particular ADJ hjic-799 52 51 sites site NOUN hjic-799 52 52 within within ADP hjic-799 52 53 the the DET hjic-799 52 54 set set NOUN hjic-799 52 55 of of ADP hjic-799 52 56 eight eight NUM hjic-799 52 57 selected select VERB hjic-799 52 58 kinases kinase NOUN hjic-799 52 59 . . PUNCT hjic-799 53 1 46(1 46(1 X hjic-799 53 2 ) ) PUNCT hjic-799 54 1 pp pp ADP hjic-799 54 2 . . PUNCT hjic-799 55 1 3 3 NUM hjic-799 55 2 - - SYM hjic-799 55 3 6 6 NUM hjic-799 55 4 ( ( PUNCT hjic-799 55 5 2018 2018 NUM hjic-799 55 6 ) ) PUNCT hjic-799 55 7 6 6 NUM hjic-799 55 8 hanáková hanáková NOUN hjic-799 55 9 , , PUNCT hjic-799 55 10 potěšil potěšil PROPN hjic-799 55 11 , , PUNCT hjic-799 55 12 bernatík bernatík ADJ hjic-799 55 13 , , PUNCT hjic-799 55 14 červenka červenka PROPN hjic-799 55 15 , , PUNCT hjic-799 55 16 rádsetoulal rádsetoulal PROPN hjic-799 55 17 , , PUNCT hjic-799 55 18 bryja bryja NOUN hjic-799 55 19 , , PUNCT hjic-799 55 20 and and CCONJ hjic-799 55 21 zdráhal zdráhal NOUN hjic-799 55 22 differences difference NOUN hjic-799 55 23 were be AUX hjic-799 55 24 observed observe VERB hjic-799 55 25 in in ADP hjic-799 55 26 terms term NOUN hjic-799 55 27 of of ADP hjic-799 55 28 the the DET hjic-799 55 29 phosphorylation phosphorylation NOUN hjic-799 55 30 profiles profile NOUN hjic-799 55 31 induced induce VERB hjic-799 55 32 by by ADP hjic-799 55 33 individual individual ADJ hjic-799 55 34 kinases kinase NOUN hjic-799 55 35 , , PUNCT hjic-799 55 36 as as SCONJ hjic-799 55 37 indicated indicate VERB hjic-799 55 38 in in ADP hjic-799 55 39 fig fig NOUN hjic-799 55 40 . . PUNCT hjic-799 56 1 1 1 X hjic-799 56 2 . . PUNCT hjic-799 56 3 based base VERB hjic-799 56 4 on on ADP hjic-799 56 5 our our PRON hjic-799 56 6 results result NOUN hjic-799 56 7 , , PUNCT hjic-799 56 8 a a DET hjic-799 56 9 “ " PUNCT hjic-799 56 10 comprehensive comprehensive ADJ hjic-799 56 11 map map NOUN hjic-799 56 12 ” " PUNCT hjic-799 56 13 of of ADP hjic-799 56 14 the the DET hjic-799 56 15 phosphorylations phosphorylation NOUN hjic-799 56 16 of of ADP hjic-799 56 17 human human ADJ hjic-799 56 18 dvl3 dvl3 NOUN hjic-799 56 19 will will AUX hjic-799 56 20 be be AUX hjic-799 56 21 created create VERB hjic-799 56 22 . . PUNCT hjic-799 57 1 acknowledgement acknowledgement NOUN hjic-799 57 2 this this DET hjic-799 57 3 work work NOUN hjic-799 57 4 was be AUX hjic-799 57 5 carried carry VERB hjic-799 57 6 out out ADP hjic-799 57 7 with with ADP hjic-799 57 8 the the DET hjic-799 57 9 support support NOUN hjic-799 57 10 of of ADP hjic-799 57 11 the the DET hjic-799 57 12 project project NOUN hjic-799 57 13 ceitec ceitec ADJ hjic-799 57 14 2020 2020 NUM hjic-799 57 15 ( ( PUNCT hjic-799 57 16 lq1601 lq1601 PROPN hjic-799 57 17 ) ) PUNCT hjic-799 57 18 funded fund VERB hjic-799 57 19 by by ADP hjic-799 57 20 the the DET hjic-799 57 21 ministry ministry PROPN hjic-799 57 22 of of ADP hjic-799 57 23 education education PROPN hjic-799 57 24 , , PUNCT hjic-799 57 25 youth youth NOUN hjic-799 57 26 and and CCONJ hjic-799 57 27 sports sport NOUN hjic-799 57 28 ( ( PUNCT hjic-799 57 29 meys mey NOUN hjic-799 57 30 ) ) PUNCT hjic-799 57 31 of of ADP hjic-799 57 32 the the DET hjic-799 57 33 czech czech PROPN hjic-799 57 34 republic republic NOUN hjic-799 57 35 under under ADP hjic-799 57 36 the the DET hjic-799 57 37 national national ADJ hjic-799 57 38 sustainability sustainability PROPN hjic-799 57 39 programme programme PROPN hjic-799 57 40 ii ii PROPN hjic-799 57 41 . . PUNCT hjic-799 58 1 the the DET hjic-799 58 2 czech czech PROPN hjic-799 58 3 infrastructurefor infrastructurefor VERB hjic-799 58 4 integrative integrative ADJ hjic-799 58 5 structural structural ADJ hjic-799 58 6 biology biology NOUN hjic-799 58 7 ( ( PUNCT hjic-799 58 8 ciisb ciisb NOUN hjic-799 58 9 ) ) PUNCT hjic-799 58 10 research research NOUN hjic-799 58 11 infrastructure infrastructure NOUN hjic-799 58 12 project project NOUN hjic-799 58 13 lm2015043 lm2015043 PROPN hjic-799 58 14 funded fund VERB hjic-799 58 15 by by ADP hjic-799 58 16 meys mey NOUN hjic-799 58 17 is be AUX hjic-799 58 18 gratefully gratefully ADV hjic-799 58 19 acknowledged acknowledge VERB hjic-799 58 20 for for ADP hjic-799 58 21 financially financially ADV hjic-799 58 22 supporting support VERB hjic-799 58 23 our our PRON hjic-799 58 24 lc lc NOUN hjic-799 58 25 - - PUNCT hjic-799 58 26 ms ms PROPN hjic-799 58 27 / / SYM hjic-799 58 28 ms ms PROPN hjic-799 58 29 measurements measurement NOUN hjic-799 58 30 at at ADP hjic-799 58 31 the the DET hjic-799 58 32 proteomics proteomic NOUN hjic-799 58 33 core core NOUN hjic-799 58 34 facility facility NOUN hjic-799 58 35 . . PUNCT hjic-799 59 1 the the DET hjic-799 59 2 support support NOUN hjic-799 59 3 from from ADP hjic-799 59 4 the the DET hjic-799 59 5 czech czech PROPN hjic-799 59 6 science science PROPN hjic-799 59 7 foundation foundation PROPN hjic-799 59 8 project project PROPN hjic-799 59 9 no no NOUN hjic-799 59 10 . . NOUN hjic-799 59 11 15 15 NUM hjic-799 59 12 - - NOUN hjic-799 59 13 21789s 21789s NUM hjic-799 59 14 is be AUX hjic-799 59 15 also also ADV hjic-799 59 16 gratefully gratefully ADV hjic-799 59 17 acknowledged acknowledge VERB hjic-799 59 18 . . PUNCT hjic-799 60 1 references reference NOUN hjic-799 60 2 [ [ X hjic-799 60 3 1 1 NUM hjic-799 60 4 ] ] X hjic-799 60 5 kersten kersten NOUN hjic-799 60 6 , , PUNCT hjic-799 60 7 b. b. PROPN hjic-799 60 8 , , PUNCT hjic-799 60 9 agrawal agrawal PROPN hjic-799 60 10 , , PUNCT hjic-799 60 11 g. g. PROPN hjic-799 60 12 k. k. PROPN hjic-799 60 13 , , PUNCT hjic-799 60 14 iwahashi iwahashi PROPN hjic-799 60 15 , , PUNCT hjic-799 60 16 h. h. PROPN hjic-799 60 17 , , PUNCT hjic-799 60 18 rakwal rakwal PROPN hjic-799 60 19 , , PUNCT hjic-799 60 20 r. r. PROPN hjic-799 60 21 : : PUNCT hjic-799 60 22 plant plant NOUN hjic-799 60 23 phosphoproteomics phosphoproteomic NOUN hjic-799 60 24 : : PUNCT hjic-799 60 25 a a DET hjic-799 60 26 long long ADJ hjic-799 60 27 road road NOUN hjic-799 60 28 ahead ahead ADV hjic-799 60 29 , , PUNCT hjic-799 60 30 proteomics proteomic NOUN hjic-799 60 31 , , PUNCT hjic-799 60 32 2006 2006 NUM hjic-799 60 33 6(20 6(20 NUM hjic-799 60 34 ) ) PUNCT hjic-799 60 35 , , PUNCT hjic-799 60 36 5517–5528 5517–5528 NUM hjic-799 60 37 doi doi NOUN hjic-799 60 38 : : PUNCT hjic-799 60 39 10.1002 10.1002 NUM hjic-799 60 40 / / SYM hjic-799 61 1 pmic.200600232 pmic.200600232 PROPN hjic-799 62 1 [ [ X hjic-799 62 2 2 2 NUM hjic-799 62 3 ] ] PUNCT hjic-799 62 4 bernatík bernatík NOUN hjic-799 62 5 , , PUNCT hjic-799 62 6 o. o. PROPN hjic-799 62 7 , , PUNCT hjic-799 62 8 šedová šedová X hjic-799 62 9 , , PUNCT hjic-799 62 10 k. k. PROPN hjic-799 62 11 , , PUNCT hjic-799 62 12 schille schille PROPN hjic-799 62 13 , , PUNCT hjic-799 62 14 c. c. PROPN hjic-799 62 15 , , PUNCT hjic-799 62 16 ganji ganji PROPN hjic-799 62 17 , , PUNCT hjic-799 62 18 s. s. PROPN hjic-799 62 19 r. r. PROPN hjic-799 62 20 , , PUNCT hjic-799 62 21 červenka červenka PROPN hjic-799 62 22 , , PUNCT hjic-799 62 23 i. i. NOUN hjic-799 62 24 , , PUNCT hjic-799 62 25 trantírek trantírek NOUN hjic-799 62 26 , , PUNCT hjic-799 62 27 l. l. PROPN hjic-799 62 28 , , PUNCT hjic-799 62 29 schambony schambony PROPN hjic-799 62 30 , , PUNCT hjic-799 62 31 a. a. NOUN hjic-799 62 32 , , PUNCT hjic-799 62 33 zdráhal zdráhal NOUN hjic-799 62 34 , , PUNCT hjic-799 62 35 z. z. PROPN hjic-799 62 36 , , PUNCT hjic-799 62 37 bryja bryja NOUN hjic-799 62 38 , , PUNCT hjic-799 62 39 v. v. ADP hjic-799 62 40 : : PUNCT hjic-799 62 41 functional functional ADJ hjic-799 62 42 analysis analysis NOUN hjic-799 62 43 of of ADP hjic-799 62 44 dishevelled3 dishevelled3 NOUN hjic-799 62 45 phosphorylation phosphorylation NOUN hjic-799 62 46 identifies identify VERB hjic-799 62 47 distinct distinct ADJ hjic-799 62 48 mechanisms mechanism NOUN hjic-799 62 49 driven drive VERB hjic-799 62 50 by by ADP hjic-799 62 51 casein casein PROPN hjic-799 62 52 kinase kinase PROPN hjic-799 62 53 1 1 NUM hjic-799 62 54 epsilon epsilon PROPN hjic-799 62 55 and and CCONJ hjic-799 62 56 frizzled5 frizzled5 PROPN hjic-799 62 57 , , PUNCT hjic-799 62 58 j. j. PROPN hjic-799 62 59 biol biol PROPN hjic-799 62 60 . . PUNCT hjic-799 63 1 chem chem PROPN hjic-799 63 2 . . PUNCT hjic-799 63 3 , , PUNCT hjic-799 63 4 2014 2014 NUM hjic-799 63 5 34(289 34(289 NUM hjic-799 63 6 ) ) PUNCT hjic-799 63 7 , , PUNCT hjic-799 63 8 23520 23520 NUM hjic-799 63 9 - - SYM hjic-799 63 10 23533 23533 NUM hjic-799 63 11 doi doi NOUN hjic-799 63 12 : : PUNCT hjic-799 63 13 10.1074 10.1074 NUM hjic-799 63 14 / / SYM hjic-799 63 15 jbc.m114.590638 jbc.m114.590638 NOUN hjic-799 63 16 [ [ X hjic-799 63 17 3 3 NUM hjic-799 63 18 ] ] X hjic-799 63 19 yanfeng yanfeng X hjic-799 63 20 , , PUNCT hjic-799 63 21 w. w. PROPN hjic-799 63 22 a. a. PROPN hjic-799 63 23 , , PUNCT hjic-799 63 24 berhane berhane NOUN hjic-799 63 25 , , PUNCT hjic-799 63 26 h. h. PROPN hjic-799 63 27 , , PUNCT hjic-799 63 28 mola mola PROPN hjic-799 63 29 , , PUNCT hjic-799 63 30 m. m. NOUN hjic-799 63 31 , , PUNCT hjic-799 63 32 singh singh PROPN hjic-799 63 33 , , PUNCT hjic-799 63 34 j. j. PROPN hjic-799 63 35 , , PUNCT hjic-799 63 36 jenny jenny PROPN hjic-799 63 37 , , PUNCT hjic-799 63 38 a. a. NOUN hjic-799 63 39 , , PUNCT hjic-799 63 40 mlodzik mlodzik NOUN hjic-799 63 41 , , PUNCT hjic-799 63 42 m. m. NOUN hjic-799 63 43 : : PUNCT hjic-799 63 44 functional functional ADJ hjic-799 63 45 dissection dissection NOUN hjic-799 63 46 of of ADP hjic-799 63 47 phosphorylation phosphorylation NOUN hjic-799 63 48 of of ADP hjic-799 63 49 disheveled dishevel VERB hjic-799 63 50 in in ADP hjic-799 63 51 drosophila drosophila PROPN hjic-799 63 52 , , PUNCT hjic-799 63 53 dev dev PROPN hjic-799 64 1 . . PUNCT hjic-799 64 2 biol biol PROPN hjic-799 64 3 . . PUNCT hjic-799 64 4 , , PUNCT hjic-799 64 5 2011 2011 NUM hjic-799 64 6 360 360 NUM hjic-799 64 7 , , PUNCT hjic-799 64 8 132–142 132–142 NUM hjic-799 64 9 doi doi NOUN hjic-799 64 10 : : PUNCT hjic-799 64 11 10.1016 10.1016 NUM hjic-799 64 12 / / SYM hjic-799 64 13 j.ydbio.2011.09.017 j.ydbio.2011.09.017 PROPN hjic-799 64 14 [ [ X hjic-799 64 15 4 4 NUM hjic-799 64 16 ] ] X hjic-799 64 17 klimowski klimowski PROPN hjic-799 64 18 , , PUNCT hjic-799 64 19 l. l. PROPN hjic-799 64 20 k. k. PROPN hjic-799 64 21 , , PUNCT hjic-799 64 22 garcia garcia PROPN hjic-799 64 23 , , PUNCT hjic-799 64 24 b. b. PROPN hjic-799 64 25 a. a. PROPN hjic-799 64 26 , , PUNCT hjic-799 64 27 shabanowitz shabanowitz PROPN hjic-799 64 28 , , PUNCT hjic-799 64 29 j. j. PROPN hjic-799 64 30 , , PUNCT hjic-799 64 31 hunt hunt PROPN hjic-799 64 32 , , PUNCT hjic-799 64 33 d. d. PROPN hjic-799 64 34 f. f. PROPN hjic-799 64 35 , , PUNCT hjic-799 64 36 virshup virshup PROPN hjic-799 64 37 , , PUNCT hjic-799 64 38 d. d. PROPN hjic-799 64 39 m. m. PROPN hjic-799 64 40 : : PUNCT hjic-799 64 41 site site NOUN hjic-799 64 42 - - PUNCT hjic-799 64 43 specific specific ADJ hjic-799 64 44 casein casein NOUN hjic-799 64 45 kinase kinase PROPN hjic-799 64 46 1ε 1ε PROPN hjic-799 64 47 - - PUNCT hjic-799 64 48 dependent dependent ADJ hjic-799 64 49 phosphorylation phosphorylation NOUN hjic-799 64 50 of of ADP hjic-799 64 51 dishevelled dishevelled ADJ hjic-799 64 52 modulates modulate NOUN hjic-799 64 53 β β NOUN hjic-799 64 54 - - ADJ hjic-799 64 55 catenin catenin ADJ hjic-799 64 56 signaling signaling NOUN hjic-799 64 57 , , PUNCT hjic-799 64 58 febs febs NOUN hjic-799 64 59 j. j. PROPN hjic-799 64 60 , , PUNCT hjic-799 64 61 2006 2006 NUM hjic-799 64 62 273 273 NUM hjic-799 64 63 , , PUNCT hjic-799 64 64 4594–4602 4594–4602 NUM hjic-799 64 65 doi doi NOUN hjic-799 64 66 : : PUNCT hjic-799 64 67 10.1111 10.1111 NUM hjic-799 64 68 / / SYM hjic-799 64 69 j.1742 j.1742 PROPN hjic-799 64 70 - - PUNCT hjic-799 64 71 4658.2006.05462.x 4658.2006.05462.x NUM hjic-799 64 72 hungarian hungarian ADJ hjic-799 64 73 journal journal NOUN hjic-799 64 74 of of ADP hjic-799 64 75 industry industry NOUN hjic-799 64 76 and and CCONJ hjic-799 64 77 chemistry chemistry NOUN hjic-799 64 78 introduction introduction NOUN hjic-799 64 79 experimental experimental ADJ hjic-799 64 80 cell cell NOUN hjic-799 64 81 culture culture NOUN hjic-799 64 82 and and CCONJ hjic-799 64 83 transfection transfection NOUN hjic-799 64 84 gel gel NOUN hjic-799 64 85 electrophoresis electrophoresis NOUN hjic-799 64 86 , , PUNCT hjic-799 64 87 protein protein NOUN hjic-799 64 88 digestion digestion NOUN hjic-799 64 89 and and CCONJ hjic-799 64 90 phosphopeptide phosphopeptide NOUN hjic-799 64 91 enrichment enrichment NOUN hjic-799 64 92 mass mass PROPN hjic-799 64 93 spectrometry spectrometry NOUN hjic-799 64 94 results result NOUN hjic-799 64 95 and and CCONJ hjic-799 64 96 analysis analysis NOUN hjic-799 64 97 identification identification NOUN hjic-799 64 98 of of ADP hjic-799 64 99 phosphorylation phosphorylation NOUN hjic-799 64 100 sites site NOUN hjic-799 64 101 phosphorylations phosphorylation NOUN hjic-799 64 102 induced induce VERB hjic-799 64 103 by by ADP hjic-799 64 104 ck1 ck1 PROPN hjic-799 64 105 and and CCONJ hjic-799 64 106 nek2 nek2 NOUN hjic-799 64 107 discussion discussion NOUN hjic-799 64 108 conclusion conclusion NOUN