IBN AL- HAITHAM J. FOR PURE & APPL. S CI. VOL.23 (1) 2010 The Effect of Rifadin Drug on Lipids Metabolism of Rabbits R. F.Abd Al-Lattif Departme nt of Biology, College of Education ,Ibn Al-Haitham , Unive rsity of Baghdad Abstract The aim of this st udy was intended to st udy the effect of rifadin drug on lip ids metabolism in the blood of the rabbits .Eight rabbits were used in the exp eriment ,they were divided into two group s ,first group (control group ) was administ rated with 15mg/ml /day normal saline for 35 days ,while the second group (treated group )was administ rated with (15/mg /kg/day ) for 35 days of rifadin( cap sule 300mg/kg).This st udy examined the influences of rifadin drug on the concentration of cholesterol , triglycerides , HDL ,LDL and VLDL in rabbits sera .The result showed that there were no significant increased (p >0.05) in cholesterol concentration in rifadin treated group comp ared with control group ,and there were no significant increase (p >0.05)in LDL and HDL concentration in rifadin treated group comp ared with control group ,where the result showed that there was a significant decrease (p <0.05)in triglycerides and VLDL concentration in rifadin treated group comp ared with control group . It was concluded that t he rifadin drug has a negative effect p roduced dy sfunction on lip ids metabolism in blood. Key words: Rifadin drug, lip ids metabolism , lipids p rofiles . Introduction Cholest erol is a fatt y substance st eroid normally found in all body cells and p lasma ,the body uses cholesterol to help build cells and p roduce hormones, it travels through the blood att ached to a p rotein , this cholesterol –p rotein p ackage is called lip op rotein , 75% of the cholesterol is bound to low density p roteins (LDL the bad ) and 25% is bound to high density p roteins (HDL the good ) . Triglycerides are a comp lex dietary lip ids comp osed of a glycerol and 3 fatt y acids ( made up of 50% fat and 50% sugar ) it is st ored mainly in the liver and it is an important source of energy , its transp orted by very low density p roteins (VLDL) and HDL [1 ]The liver represents the main site of cholesterol and triglyceride metabolism [2], in the blood lip id are transp orted as a globular high molecular weight lip op rotein p articles , lip ids contents gradually undergo metabolism in various tissues including the liver and blood . Cholest erol and HDL are p roduced in the liver and p lasma [3]. The lip id p rofiles include the test :- total cholesterol , triglyceride , HDL , LDL and VLDL [4] . Rifadin is an imp ortant drug in the treatment of tuberculosis [ 5],[6] its an affective antibiotic against gram –p ositive bacteria inducing mycobacterium [7] ,rifadin inhibits DNA –dep endent RNA –p olymerase activity and protein sy nthesis in bacteria [8], it has a significant activity against Neisseria M enoigitidis isolated [9] rifadin is an enzy me induce and enhance formation of reactive metabolism [10]and an oral dose of 600mg once or twice daily can eliminate the majority of meningococci from caries[5]. Rifadin drug found to cause an increasing in the rate of T3 and T4 metabolism [11] anemia [12], thrombocy top enia and leucopenia [9] , reduced the level of glutathione [13] and the level of lip ids [14]. The aim of this st udy was intended to st udy the effect of rifadin drug on lip ids metabolism in the blood of the rabbits. IBN AL- HAITHAM J. FOR PURE & APPL. S CI. VOL.23 (1) 2010 Material and Method Eight mature rabbits weighting a bout (1200─ 1700)gm were used in this exp eriment .Animals were kept under slandered laboratory conditions and were given food and water ,animals were divided into two group s and were subjected to exp erimental schedule as follows :first group (control group ) was administ rated daily with (15gm/ml/day ) normal saline for 35 days and used as a control group , second group (treated group ) was administrated daily with (15gm/kg/day ) of rifadin cap sule (300gm/kg) from(M umbai , ajanta) .This schedule continued for consecutive for thirty five days .Twenty four hours after the last treated ,p erip heral blood was collected from the rabbits and serum samp les were sep arated and were freezed until assay ed .Plasma concentration of cholesterol ,triglyceride ,HDL,LDL and VLDL were determined by using sp ectrop hotometer. Re sult and Discussion The result of st atist ics analysis showed no significant increase (p >0.05)in cholesterol concentration in treated group (61.0±4.6) mg/dl as comp ared with control group (59.0±3.0) mg/dl, also there was no significant increase (p .0.05)in HDL concentration in treated group (35.0±6.0) mg /dl as comp ared with control group (28.7±3.5) mg /dl ,see figures (1),(2) ,also was‘nt found any significant increase (p >0.05) in LDL concentration in treated group (7.66±4.9) mg /dl as comp ared with control group (3.5±0.5) mg/dl, see figure (3) .From the a above result one can conclude that rifadin drug caused an increase in the level of cholesterol , HDL and LDL and that is due to rifadin wt ich causes cholestasis ,cholestasis is a bile flow st agnations which may result from a failure in the secretary transp ort in the hep atocy te or in the ductular cells and that caused increase in the level of cholesterol (115) ,rifadin caused liver injury [12] ,[17] ,and toxicity to the liver [15] ,[8] liver represents the main site of the cholesterol and HDL sy nthesis and storage [2] . It was found from that there was a relationship between the level of cholesterol and the level of HDL and LDL, and that is because HDL and LDL represent a very imp ortant role in the transp ortation of cholesterol in blood to the tissues from the liver [4]. The result showed a significant decreased (p <0.05) in triglycerides in treated group (93.6±14.6) mg /dl as comp ared with control group (151.0±34.5) mg/dl .Also it was found a significant decrease (p <0.05) in VLDL concentration in treated group (`18.3±3.2) mg/dl as comp ared with the control group (25.0±2.0) mg/dl , see figures (4) ,(5) that result is because rifadin drug caused a decrease in the level of triglycerides [19], also from the above result it was found that there was a relationship between VLDL is concentration and triglycerides concentration because VLDL very rich with triglycerides and its level became in the same side of the triglycerides concentration that means when the level of triglycerides is decreased the level of VLDL decreased also in the blood [4],the p lasma of triglycerides represent a biomarker for hepatot oxicity [19] because the liver represents the main site of the sy nthesis and storage of triglycerides [2] ,all that change in lip id p rofiles is due to the effect of rifadin drug in the liver [16] . It was concluded that rifadin drug decreases the concentration of triglycerides and VLDL, and increases the concentration of cholest erol , LDL and HDL in the blood, that means rifadin drug dy sfunction the lip ids metabolism in the blood . Re ferences 1. Cardett, Pat.(2007).Wellness Lab Rep orts & Rx .Dr of Wellness com . 2. Schroeder ,S.B.(2005). Chemical inhibition of the thy roid gland and its effects on E.coli O157-H7 fecal shedding p alierns in sheep .A t hesis submitt ed to T exas University . 3. Kumana ,C. R. ;Lam,K.S. and Janus, E.D.(1996) .J HK coll Cardiol 4. IBN AL- HAITHAM J. FOR PURE & APPL. S CI. VOL.23 (1) 2010 4. Abd Almahdi ,R .(2003). The effect of sub clinical Hy p othy roidism on lip id profile and lip id per oxidation factors .A t hesis submitt ed to college of Science .University of Baghdad. 5. M anosuthi ,W .(2008) .BM C infections Disease .8(136): 1-7. 6. Koju ,D.; Rao, B.,S.; Shrestha,B. ;Rajani ,Sha and M akaj,R. .(2005) . Journal of Scince 1(1):1-5. 7. Chen,J. and Ray mond,K .(2006). M akaj.Journal of Scince 5(3) : 14 8. Gz aanko, L.(2003).M ID 30 :anti fungal &anti tuberculosis agents .//health sciences. Columbia .edu./dept/ps/2007/mid/2006/M ID30.p df 9. M ,n,^h.,roH,hu.(1999).Cener for drug evaluation and research. BenVenue laboraties.inc 10. Razdan ,R. and Amar ,Deo,M .,J.(2008) . Pharmacognosy M agazine .4( 15) : 0975- 1296. 11. Ormrod , P .(1998).Chemotherap y and management of tuberculosis in the united kingdom: recommend dation 1998.Thorax ,53 :537-548. 12. M ahmood,k .(2007).Hep atot oxicity with anti tuberculosis drugs :the risk factors .Park J M ed Sci ,23(1) :1 -7. 13. Nigama ,P. and M ukhija, R.D.(1982).Heap ortective role of indigenous drug liv-52 in tepromatous leprosy .Hansen logia in denationalize ,Brazil ,7(1) :36. 14.Kumer,R. J.; Rajesh R. and M eena, B.D.(2008) .Ant i hepatot oxic effect of p icrorhiza on mitoc hondriald defuse sy st em in anti tuberculosis (isonazid and rifampicin )induced hepatitis in rats .Journal of M edicinal Plants research ,2(1) :017 -019. 15. Rodriuez ,A.E.(2003).Annals of hematology .2(4):150- 158. 16. Peradhx ,S.C.and Cirish ,C.(2006) .Indian J M ed Res .124: 491-504. 17. M inihin ,P.Y. and Gandhi ,T.R .(2008).Pharmacognosy Review . 2 ( 3):1-4. 18. Pari, L. and kumar ,w.A. (2002).Journal of M edicinal Food .5( 3): 171-177. 19. Provost , J.P.; Hanton ,G. and Lie Net,J. (2003) .Comp arative clinical p athology . 12( 2): 95-101. Table (1):-Effect of rifadin drug(15mg/kg/day) on choleste rol, triglycerides, HDL, LDL and VLDL in te sti ng and control groups. VLDL mg/dl LDL mg/dl HDL mg/dl Triglycerides mg/dl Cholest erol mg/dl Group s 18.3±3.2 7.66±4.9 35.0±6.0 93.6±14.5 61.0±4.6 Treated with rifadin (15mg/kg/day 25.0±2.0 3.5±0.5 28.7±3.5 151.0±34.5 59.0±3.0 Control IBN AL- HAITHAM J. FOR PURE & APPL. S CI. VOL.23 (1) 2010 59 61 58 58.5 59 59.5 60 60.5 61 cholester ol concentr ation mg/dl c t control and treated groups Fig.(1): Effect of rifadin(15/mg/kg/day) on chol esterol concentratio in treated and control groups. 7.66 3.5 0 1 2 3 4 5 6 7 8 LDL concentr a ti on m g/dl t c contr ol and tre ated grou ps Fig. (2): Effect of rifadin(15mg/kg/day) on LD L concentration in treated and control groups. IBN AL- HAITHAM J. FOR PURE & APPL. S CI. VOL.23 (1) 2010 28.7 35 0 5 10 15 20 25 30 35 HDL concentrati on mg/dl c t control and treated groups Fig.(3): Effect of rifadin(15mg/kg/day) on HDL concentration in treated and control groups 25 18.3 0 5 10 15 20 25 VLDL concentrati on mg/dl c t control and treated groups Fig. (4): Effect of rifadin(15mg/kg/day) on VLD L concentration in treated and control groups IBN AL- HAITHAM J. FOR PURE & APPL. S CI. VOL.23 (1) 2010 151 93.6 0 20 40 60 80 100 120 140 160 triglycride concentrati on mg/dl c t control and treated groups Fig. (5): Effect of rifadin(15mg/kg/day) on triglycerides concentration in treated and control groups 2010) 1( 23مجلة ابن الھیثم للعلوم الصرفة والتطبیقیة المجلد ایض الدهون في االرانب في تاثیرعقارالریفادین فائق عبد اللطیف أرش جامعة بغداد، كلیة التربیة ابن الهیثم ، قسم علوم الحیاة الخالصة ذة ثمانیـة ارانـب فـي هـ تاسـتخداماذ ،ایض الدهون في االرانـبفي هدفت الدراسة الحالیة دراسة تاثیر عقار الریفادین محلــوال ) یـوم/مــل /ملغـم 15( وتــم تجریعهـا ب) مجموعــة السـیطرة (مجمــوعتین المجموعـة االولـى علـى الدراسـة ثـم قســمت تم معاملتهـا بعقارالریفـادین فـ) المجموعـة المعاملـة (امـا المجموعـة الثانیـة , "ایومـ 35مـدة normal saline فسیولوجیا ملحیا تركیـز فـي شـملت الدراسـة تاثیرعقـار الریفـادین . "ایومـ 35مـدة ) یـوم/كغـم /ملغـم 15(وبجرعة ) كغم /ملغم 300كبسول ( pواظهـرت النتـائج عـدم حصـول زیـادة معنویـة، VLDL وHDL , ، LDLالـدهون الثالثیـة ,الكولیسـترول فــي ) (0.05< ففـي حـین ،دین مقارنـة مـع مجموعـة السـیطرةفي المجموعة المعاملة بالریفـاHDL وال LDLتركیز كل من الكولیسترول وال p) اظهرت النتائج و جـود نقصـان معنـوي فـي المجموعـة المعاملـة VLDL فـي تركیـز كـل مـن الـدهون الثالثیـة وال (0.05> ــــــیطرة ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ـــــع مجموعةالسـ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــة مــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ـــادین مقارنـــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ ــــ . بالریفـــ .یتضح من هذه الدراسة ان لعقار الریفادین تاثیرا سلبیا یودي الى حدوث اختالل ایض الدهون في الدم