Microsoft Word - 57-80 57 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 Synthesis, Antibacterial and Antifungal Activities for Novel Derivatives of 2,2'-(((1-benzylbenzoimidazol-2-yl) methyl) azanediyl) bis (ethan-1-ol) Aziz Latif Jarallah Department of Medical Lab, Technology, Esraa University College Khalid Fahad Ali Raied Mustafa Shakir raiedalsayab@yahoo.co.uk Shaimaa Abed Saoud Department of Chemistry, College of Education for Pure Science Ibn Al-Haitham, University of Baghdad, Baghdad, Iraq. Article history: Received 19 November 2018, Accepted 17 October 2018, Publish January 2019 Abstract The compound 2,2'-(((1H-benzo(d) imidazol-2-yl)methyl)azanediyl)bis(ethan-1-ol) was reacted with benzyl bromide to afford compound (1) which used as row material to prepare a series of compounds through condensation reaction, the starting compound were reacted with tosyl chloride to protect the OH group to afford compound 2, then reacted benzyl bromide to produce compound (2), then the compound (2) treated with three compounds (2- mercaptobenzthiazole, 2-mercaptobenimidazol and 2-chloromethyl benzimidazole) to form compounds 3a, b, 4a,b and 5a,b respectively. In the another step the click reaction of compound 2, 2'-(((1H-benzo(d)imidazol-2-yl) methyl) azanediyl)bis(ethan-1-ol) with Propargyl bromide produce compound 6 which reacted with sodium azide or benzyl azide to afford the compounds 7 and 8. The synthesized compounds were characterized and measured the physical properties via the FT-IR, HNMR, besides to the CHN analysis. These newly compounds were screened their antibacterial and antifungal activity. Compounds 1, 2a and 8 showed significant antibacterial activity as well these compounds exhibited either low or moderated antifungal activity. Keywords: Benzimidazole Derivatives, diethanolamine, Benzothiazole, Triazole, Antibacterial, Antifungal. 1.Introduction The heterocyclic compounds are considering very important compound in many fields like the agriculture, industry and medicine application, the benzimidazole compounds are derivatives from the heterocyclic compounds Benzimidazole can be described as fused bicycle between benzene and imidazole. The first synthesis of benzimidazole had been reported during 1872 by Hoebrecker whom synthesized 2,5-dimethylbenzimidazole and 2,6- dimethylbenzimidazole by the reduction of 2-nitro-4 methylacetanilide [1,2] the benzimidazoles and their derivatives exhibited remarkable biological activity and significant pharmacophore structure in therapeutic activities like antiulcers[3], antihypertensives, analgesic, anti-inflammatory [4,5], antiviral ]7,6[ , antifungals [8], anticancers ]9 -11[ , and 58 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 antihistaminics [12]. The most prominent benzimidazole compound in nature is N-ribosyl- dimethyl benzimidazole, which known as ligand for cobalt in vitamin B12 [13-15]. Due to the significant importance of benzimidazoles, a large numbers of methods have been reported for synthesizing benzimidazole derivatives and According to high biological activities of benzimidazole derivatives new benzimidazole containing some heterocyclic rings and test in vitro their preliminary antibacterial and antifungal activities. 2. Experimental All chemicals and solvents utilized brand Sigma-Aldrich, Fisher and Merck to synthesize the target compounds. The synthesized compounds were characterized and measured the physical properties via the FT-IR, HNMR, spectroscopies and melting points. 2.1. Synthesis of 2,2'-(((1-benzylbenzoimidazol-2-yl) methyl)azanediyl)bis(ethan-1-ol) (1). (1) Benzyl bromide (4.1 g, 2.4 mmol) was added to a suspension of 2,2'-(((1H-benzo(d)imidazol- 2-yl) methyl) azanediyl)bis(ethan-1-ol (2.35 g, 10 mmol) in 25 mL of acetone and anhydrous potassium carbonate (1.38 g ,10 mmol) drop wise within 30 mins. The mixture was refluxed for 48 hours and the excess of solvent was removed under reduce pressure. 50 mL of distilled water was added then transferred to a separating funnel. The mixture was extracted 25 × 3 by dichloromethane DCM. The combined organic layer washed with water and brine then dried under magnesium sulfate. After evaporating the solvent, the residue was purified by column chromatography ethyl acetate - hexane (5: 1) to afford 2.4 g (74%) as brown gummy precipitant. 2.2. Synthesis of (((1-benzyl-1H-benzo[d]imidazol-2-yl) methyl) azanediyl)bis(ethane- 2,1-diyl) bis(4-methylbenzenesulfonate)(2a) and 2-(((1-benzyl-1H-benzo[d]imidazol-2- yl)methyl)(2-hydroxyethyl)amino)ethyl 4-methylbenzenesulfonate (2b ). The 2.35 g (10 mmol) from compound 1 was taken in 40 mL dry triethylamine was stirred at 0 C for 15 mins. Tosyl Chloride (6.2 g, 32.52 mmol) was added in small portion in period of two hours with continues stirring and maintain temperature at 0 ₒC. after fulfillment the addition of the mixture is left to stirring at ambient temperature. The mixture poured into 50 mL crushed ice then acidified by 10% hydrochloric acid to pH = 5. Chloroform 100 mL is added, the mixture is transferred to separating funnel and extracted twice. The combined organic layer washed with distilled water several times then washed with brine. After daring the organic layer under anhydrous magnesium sulfate and evaporated under reduced pressure. The crude product was purified by column chromatograph using ethyl acetate - hexane (1: 8) 59 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 as eluent to obtain 4.1 g of compound 2a as brown precipitant and 1.83 g of compound 2b as brown precipitant. 2 a 2 b 2.3. Synthesis of 2-(benzo[d]thiazol-2-ylthio)-N-(2-(benzo[d]thiazol-2-ylthio)ethyl)-N-((1- benzyl-1H-benzo[d]imidazol-2-yl)methyl)ethanamine (3a) and 2-((2-(benzo[d]thiazol-2- ylthio)ethyl)((1-benzyl-1H-benzo[d]imidazol-2-yl)methyl)amino)ethyl 4- methylbenzenesulfonate (3b ). 3a 3 b Benzimidazole-2-thiol (3.35 g, 4 mmol) was added with a few portions to a solution of 2a (1.4 g, 2 mmol) in dimethyl sulfoxide DMSO and sodium hydroxide (0.16ng, 4 mmol) in normal room temperature. The mixture was heated to 100 ₒC for 5 hrs. On cooling 100 mL distill water is added then the mixture acerbated at fridge overnight. The precipitated is collected, dried at 50 ₒC. The crude compound was purified by column chromatography ethyl acetate – hexane (1: 11) to obtain brown gummy priciptant.51% of compound 3a and 30 % of compound 3b as brown gummy precipitant. 60 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 2.4. Synthesis of 2-((1H-benzo[d]imidazol-2-yl)thio)-N-(2-((1H-benzo[d]imidazol-2- yl)thio)ethyl)-N-((1-benzyl-1H-benzo[d]imidazol-2-yl)methyl)ethanamine (4a)and 2-((2- ((1H-benzo[d]imidazol-2-yl)thio)ethyl)((1-benzyl-1H-benzo[d]imidazol-2- yl)methyl)amino)ethyl 4-methylbenzenesulfonate (4b ). 4a 4 b The target compounds were synthesized utilizing the same method mentioned in synthesized the compounds 3a, 3b and using 4 mmol of benzimidazole-2-thiol instead of Benzathiazole-2-thiol. The crude compound was purified by column chromatography ethyl acetate – hexane (1: 1) to afford 47% of compound 4a as brown gummy precipitant and 33 % of compound 4b as brown Gummy precipitant. 2.5. Synthesis of 2-((1H-benzo[d]imidazol-2-yl)methoxy)-N-(2-((1H-benzo[d]imidazol-2- yl)methoxy)ethyl)-N-((1H-benzo[d]imidazol-2-yl)methyl)ethanamine (5a)and 2-((2-((1H- benzo[d]imidazol-2-yl)methoxy)ethyl)((1-((1H-benzo[d]imidazol-2-yl)methyl)-1H- benzo[d]imidazol-2-yl)methyl)amino)ethanol (5b ). 5 a 5 b 2-Chloromethyl benzimidazole (1 g, 6 mmol) was added with small portions to a solution of compound 1 (0.71g, 3 mmol) in dry pyridine at ambient temperature. The mixture was heated under reflux overnight. Upon cooling the mixture poured into 50 mL crashed ice then acidified to pH 6-5 by 5% cooled solution of hydrochloric acid. The resulting product was extracted with dichloromethane DCM (30 x 3) and washed with water then brine. The combine organic layer dried under magnesium sulfate and evaporated, the crude product 61 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 purified by column chromatograph to afford brown gummy precipitant yield 44% of compound 5a and 34 % of compound 5b as brown gummy precipitant. 2.6. Synthesis of 2,2'-(((1-(prop-2-yn-1-yl)-1H-benzo[d]imidazol-2- yl)methyl)azanediyl)diethanol (compound 6 ). (6) Propargyl bromide (2.6 g, 22 mmol) is added dropwise to a solution of 2,2'-(((1H- benzo[d]imidazol-2-yl)methyl)azanediyl)diethanol (4.7 g, 20 mmol) in 10 mL of dimethyl sulfoxide with sodium hydroxide (0.8 g, 20 mmol) at ambient temperature. The mixture is heated to 70 ₒC under reflux for 6 hrs. After cooling the mixture, it quenched with 40 mL distilled water then transferred to separate funnel and extract three times with 100 mL of dichloromethane DCM. The organic layer is washed with distilled water several time, dried under anhydrous magnesium sulfate. The organic solvent is removed under reduced pressure. The crude product was purified by column chromatography using ethyl acetate – hexane (1: 1) As eluent to afford yellow precipitant with 87% yield. 2.7. Synthesis of 2,2'-(((1-((1H-1,2,3-triazol-4-yl)methyl)-1H-benzo[d]imidazol-2- yl)methyl)azanediyl)diethanol (compound 7 ). (7) Sodium azide (0.2 g, 3.1 mmol) is added to a stirring solution of compound 6 (0.41g, .1.5mmol) in 25 mL Methanol - H2O (5:1). Copper sulfate five hydrates (0.08 g, 0.5 mmol) is added followed by (0.59 g, 3 mmol) of sodium ascorbate. The mixture has left under vigorously stirring at ambient temperature for 24 hours. The resulting solution is filtered, evaporated under reduced pressure. 62 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 The crude product was purified by column chromatograph using ethyl acetate – hexane (5: 1) to give white precipitant of yield 66%. 2.8. Synthesis of 2,2'-(((1-((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)-1H-benzo[d]imidazol- 2-yl)methyl)azanediyl)diethanol (8). 8 Freshly prepared Benzyl azide (0.2 g, 1.5 mmol) is added to a stirring solution of compound 6 (0.41g, 1.5 mmol) in 25 mL Methanol Copper sulfate five hydrate (0.08 g, 0.5 mmol) then followed by adding (0.59 g, 3 mmol) of sodium ascorbate. The mixture left under vigorously stirring at ambient temperature for 24 hours. The resulting is filtered, evaporated under reduced pressure. The crude product was purified by column chromatography using ethyl acetate – hexane (5 : 1) as eluent to give white precipitant. 3. Results and Discussion 3.1 Chemistry The compound 1 is synthesized by benzylation reaction in acetone and in the presence of potassium carbonate as demonstrated in Scheme 1. This compound is characterized by FTIR and 1H NMR besides to the CHN analysis. The FTIR spectrum of this compound showed the broad band for OH at 3404 cm-1, while the NH of benzimidazole was disappeared from 3384 cm-1. The band of C=N located at 1651 cm-1 and the C=C at 1485 cm-1 as displayed in Figure 1. The 1H NMR spectrum of this compound showed multiplet peak at 4.16 ppm for nine protons, eighth of them for CH2 and one broad peak for OH. The second OH did not appeared and that could be due to engage with intramolecular hydrogen bonding or to deuterium exchange[16-18]. The CH2 of benzyl appeared as singlet at 4.75 ppm with integral of two protons. Moreover, the singlet of CH2 attached with benzimidazole appeared at 5.49 ppm. The aromatic protons located as multiplet peak at 6.84-7.66 ppm as displayed in Figure 2. 63 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 Scheme 1: Route of synthesized compounds 1, 2a-b, 3a-b, 4a-b, 5a-b, 6 ,7 and 8. Tosylation of compound 1 in pyridine afforded two products. The major one was with two tosyl group, while the minor one was with on tosyl group. Both compounds 2a and 2b were characterized from their FTIR and 1H NMR spectra. The FTIR for 2a exhibited disappearance of the hydroxyl group and that was good indicator for the success of tosylation reaction. As well as the rest band was located at their expected region. For instance, the CH aliphatic and C=C were located at 2900 and 1662 cm-1 respectively as depicted in Figure 3. The 1H NMR of compound 2a showed singlet for six protons at 2.3 ppm attributed to two CH3 of tosyl as well multiplet peak at 3.42-3.5 ppm for eight protons for four CH2 group. The CH2 of benzyl is located at 4.27 ppm as singlet peak and the CH2 attached benzimidazole located at 4.29 ppm as singlet peak. The aromatic protons of the benzimidazole and benzyl appeared as multiplet at 6.4-6.69 ppm while the aromatic protons of two tosyl group appeared as two doublet peak at 7.22 ppm and 7.52 ppm respectively as shown in Figure 4. Existence of hydroxyl group at FTIR of compound 2b besides the integration of methyl group of tosyl at 2.27 ppm and the appearance of OH in 1H NMR spectrum for this compound confirms that one tosyl group was substituted. Furthermore, the results of CHN analysis harmonized with the proposed structure, as shown in Figure 5. 64 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 The compounds 3a and 3b were characterized by FTIR and 1H NMR spectra. The FTIR spectrum of compound 3a showed the aromatic CH at 3097 cm-1 and the aliphatic CH at 2924-2852 cm-1.the band of C=N is located at 1610 cm-1 and the C=C at 1516, 1425 cm-1 as demonstrated in Figure 6. The 1H NMR of this compound showed disappearing to the CH3 of tosyl group as well the four group of CH2 appeared as multiplet: with integration equal to eight protons. The two CH2 at high field 4.16-4.26 ppm with integral of four protons at 4.97 and 5 ppm attributed to CH2 of benzyl and to CH2 attached the benzimidazole ring respectively. The integration of aromatic protons was identical to judge that the two benzothiazole substituted as shown in Figure 7. Expectance singlet at 2.3 ppm for three protons of CH3 belong to tosyl quit enough evidence that the minor product (3b) is substituted with one group of benzothiazole. The FTIR and 1H NMR was depicted in Figures 8. and 9. respectively. 2- Thiobenzimidazole also was substituted as depicted in scheme 1. As well as this reaction proceed as earlier mentioned and afforded two products 4a as major product while 4b was isolated by column chromatography as minor product. Both compounds (a, b) were characterized by FTIR and 1H NMR spectra. The FTIR of compound 4a exhibited the NH of benzimidazole at 3105 cm-1 and the CH aliphatic at 2979. 2873 cm-1. The band of C=N was located at 1633 cm-1 and the C=C at 1606, 1531 cm-1 as demonstrated in Figure 10. The 1H NMR of compound 4a showed three multiplet peak at high magnetic field two of them for four CH2 of ethanol amine while the third one belongs CH2 of benzyl and CH2 attached Benzimidazole at position two. The aromatic protons were assigned as multiplet with integral equal seventeen protons. The interested NH of benzimidazole appeared as two protons at low magnetic field with integration equal to two. These two peaks refer that the two NH were unsymmetrical due to the geometrical orientation[19-20] as demonstrated in Figure 11. The FTIR of compound 4b (Figure 12.) showed the band of NH benzimidazole at 3323 cm-1 . The band of the CH aromatic was located at 3032cm-1 and the CH aliphatic at 2927, 2852 cm- 1. The band of C=N and C=C was located at 1620, 1568 and 1460 cm-1 respectively. Furthermore, the 1H NMR of compound 4b illustrated the structure and confirmed existence one tosyl group. Whereas the peak at 2.21 ppm with integral three refer for one tosyl group. All rest protons appeared at their expected region with their expected integration (Figure 13.). The route of synthesis of compounds 5a and 5b is demonstrated in scheme 1. These compounds were identified from their FTIR and 1H NMR spectrum besides to the CHN analysis. The FTIR spectrum of compound 5b displayed the hydroxyl group and rising new band at 3141 cm-1 for NH of benzimidazole. As well the CH aliphatic were assigned at 2981, 2931 cm-1. The strong band located at 1670 cm-1 attributed to C=N cm-1 and the C=C located at 1614 cm-1 as shown in Figure 14. The 1H NMR spectrum of this compound illustrated the structure by exhibiting all expected peak and their correct integrations. For instance, the 1H NMR spectrum showed multiplet peak at 4.19-4.93 ppm for fourteen protons attributed to seven set of CH2 as well as the integration of aromatic proton for the multiplet peak at 6.91-7.71 ppm equal seventeen protons. 65 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 These two multiplet peaks harmonized with the structure. Furthermore, the two NH of benzimidazole located at 10.39 ppm for wit integration equal to two protons as depicted in Figure 15. The results of CHN analysis also were in agreement with IR and 1H NMR results. The FTIR Figure 16. of the second compound 5b clarified the existence of the hydroxyl group through the band at 3244 cm-1 as well existence of the NH of benzimidazole. These bands refer to reaction one hydroxyl group with 2-chloromethyl benzimidazole. The band of aliphatic CH located at 2958 cm-1 and the C=N at 1647 cm-1 moreover the band of C=C were located at1603, 1520 cm-1. The 1H NMR spectrum of this compound exhibited one broad peak at 3.32 ppm integral equals one for alcoholic hydroxyl as well as multiplet peak at 5.22-5.64 ppm for five set of CH2. The two singlet peaks appeared as multiplet at 5.83-5.95 ppm due to overlap for two set of CH2, CH2OH and CH2 of benzyl group as demonstrated in Figure 17. Finally, the multiplet peak at 6.50-7.30 ppm showed integration equals thirteen aromatic protons and the integration of NH benzimidazole ring was one proton assigned at 10.00 ppm. All these peaks illustrate the proposed structure and it in agreement with the results of CHN analysis. Reaction of Propargyl chloride with compound 2,2'-(((1H-benzo[d]imidazol-2- yl)methyl)azanediyl) diethanol in dimethyl sulfoxide DMSO and sodium hydroxide afford compound 6 as depicted in Scheme 1. This compound was characterized by FTIR and 1H NMR. The FTIR showed broad band at 3417 cm -1which consists of the two groups of alcohol and the band of acetylenic hydrogen (≡CH), as well as the aromatic CH band and the aliphatic CH band were located at 2999,2817 cm-1 the band of C≡C was located at 2224 cm-1. The band of C=N assigned at 1658 cm-1 and the C=C assigned at 1599, 1533 cm-1 as demonstrated in Figure 18. The 1HNMR spectrum confirm success of the reaction where it displays the propynyl group. The protons of this group were located as triplet at 3.35 ppm with J= 6.5 Hz for ≡CH and doublet at 4.285 ppm with J=7 Hz due to long range coupling[21]. The 1H NMR spectrum showed two triplets at 3.34-3.38 ppm and 5.40-5.42 ppm attributed to NCH2CH2 and CH2OH respectively. The CH2 attached position two of benzimidazole located as singlet peak at 5.83 ppm and the aromatic protons appeared as multiplet peak at 7.45-7.52 ppm. The integrations of all protons were compatible with number of protons in chemical structure of compound 6 as depicted in Figure 19. The compound 7 was synthesized by click chemistry[22,23] utilizing sodium ascorbate and CuSO4.5H2O as depicted in Scheme 1. The proposed mechanism of this reaction was in agreement with the description of by Himo et al [24] and as shown in scheme 2 below : 66 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 Scheme 2: Proposed mechanism of Synthesis of compound 7 This compound was characterized by FTIR and 1H NMR spectra besides to the CHN analysis. All data confirm the success of the reaction. The FTIR showed disappearance of both band of propynyl group (≡CH and C≡C) as well rising new band for NH of 1,2,3-triazole ring at 3323 cm-1 while the OH appeared at 3496 cm-1. The CH aliphatic located at 2970, 2935 cm-1 and the C=N located at 1633 and C=C at 1562,1527cm-1 as depicted in Figure 20. The 1H NMR spectrum of compound 7 Figure 21. exhibited convert the doublet of CH2 of propynyl to singlet at 5.12 ppm besides to the change in pattern of aromatic protons and exhibited integration equal five protons quit good evidence for the success of the cyclization reaction. As well as all rest peaks located at their expected area. Although the one hydroxyl group wasn’t recognized in this spectrum, It could be either masked with the peak of water for DMSO-d6, or undergoes deuterium exchange [25 , 26] The percentage of nitrogen in CHN analysis (Table 1.) besides to the percentage of H and C were too persuasive with convert the propynyl group to corresponding 1,2,3-triazole ring. The compound 8 was synthesized by using the same method mentioned earlier. The difference in this reaction was by utilizing freshly synthesized of benzyl azide instead of sodium azide. The route of synthesizing this compound illustrated by scheme 1. This compound successfully characterized by FTIR and 1H NMR besides to the elemental analysis. The FTIR spectrum of is exhibited disappearing of the bands of propynyl, as well as the band of OH at 3191 cm-1. The aliphatic CH bands were located at 2956, 2871 cm-1 and the C=N at 1610 cm-1 besides to C=C bands were located at 1543, 1477 cm-1 as demonstrated in Figure 22. The 1H NMR spectrum displayed multiplet at 7.07 –7.25 ppm for six aromatic protons which was attributed to the benzimidazole and two protons of Meta benzyl. Furthermore, one triplet at 7.45 ppm with J=8.22 Hz for one proton of para benzyl besides two protons of ortho benzyl located at 7.81 ppm as doublet with J=7.97 Hz. Two singlet peaks for two CH2 groups attached with benzimidazole ring were assigned at 5.65 and 5.74 ppm. Five protons for two CH2 attached with hydroxyl and on hydroxyl located as multiplet peak at 4.12-4.23 ppm. The second hydroxyl did not recognize for the same reasons mention earlier. The triplet of N(CH2CH2OH)2 assigned at 3.35 with J =6.86 Hz as demonstrated in Figure 23. 67 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 Table 1. Elemental Analysis (Theoretical and Practical Values) for synthesized Compounds Formula S % O % N % H % C % CHN Analysis Compounds No C19H23N3O2 - 9.83 12.91 7.12 70.13 Theoretical Value 1 - 9.64 13.12 7.03 70.42 Practical Value C33H35N3O6S2 10.12 15.15 6.63 5.57 62.54 Theoretical Value 2a 10.27 15.24 6.82 5.90 62.33 Practical Value C26H29N3O4S 5.68 13.34 8.76 6.10 65.11 Theoretical Value 2b 5.87 13.11 8.98 6.24 65.56 Practical Value C33H29N5S4 20.56 - 11.23 4.69 63.53 Theoretical Value 3a 21.03 - 11.54 4. 98 63.21 Practical Value C33H32N4O3S3 15.30 7.63 8.91 5.13 63.03 Theoretical Value 3b 15.39 7.51 8.34 5.48 63.52 Practical Value C33H31N7S2 10.87 - 16.62 5.30 67.21 Theoretical Value 4a 10.57 - 16.41 5.55 67.84 Practical Value C33H33N5O3S2 10.48 7.85 11.45 5.44 64.79 Theoretical Value 4b 11.13 7.29 11.76 5.82 65.11 Practical Value C35H35N7O2 - 5.46 16.74 6.02 71.77 Theoretical Value 5a - 5.97 16.85 6.47 71.95 Practical Value C27H29N5O2 - 7.02 15.37 6.42 71.19 Theoretical Value 5b - 7.39 15.76 6.36 71.04 Practical Value C15H19N3O2 - 11.71 15.37 7.01 65.91 Theoretical Value 6 - 11.98 15.41 7.25 66.17 Practical Value C15H20N6O2 - 10.11 26.56 6.13 55.95 Theoretical Value 7 - 10.26 26.89 6.74 65.12 Practical Value C22H26N6O2 - 7.87 20.68 4.45 65.01 Theoretical Value 8 68 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 3.2 Antimicrobial Susceptibility and Antifungal Activity Antimicrobial susceptibility testing is one of the factors could be utilized in drug discovery, epidemiology and expectation of therapeutic value. The Antimicrobial Susceptibility was carried out according to procedure of McFarland et al. [27]. Two groups of Bacterial isolates were selected for biological activity. The first group is Gram Positive Bacteria (Vancomycin – Resistant Staphylococcus aureus (VRSA), while the second group is gram negative bacteria (Enterotoxigenic Eschrichia coli (ETEC), Psedumonas, Shigella and Vibrio). The antibacterial activity of the synthesized compounds was determined by minimum inhibitory concentration (MIC) method according to Flórez et al. (2006) [28]. The fungal conidia were harvested by scraping the sporulating colonies and suspended in sterile distilled water containing 1.0% Tween80 (v/v aqueous solution, as wetting agent) [29]. The conidia were suspended in sterile ddH2O. The resulted conidial suspensions were filtered for hyphal debris, a sterilized piece of clothes is used, and centrifugation the suspension for 5 min at 3000 rpm then washed two times with 0.05% Tween80 or ddH2O with intervening centrifugation. The Resulting conidia are resuspended in 1.0% Tween80 or ddH2O and the numbers of conidia are determined by using a haemacytometer before they were diluted with sterile water containing 1.0% Tween80 or ddH2O to reach the appropriate concentrations (1 × 106, 1 × 107 and 1 × 108 conidia/ml-1). Conidial viability is examined before to the experiments by placing three drops of a 1 × 106 conidia spores/ml-1 suspension onto agar medium followed by incubation in 30°C for 24h, after which, their germination is examined under a microscope (10 X) by observing > 90% growth for all isolates. Currently, microbial diseases are causing problems world-wide, because of their resistance to antimicrobial agents. A variety of clinically important species of microorganisms has become an important health problem globally[30] .One way to fight this challenge is the suitable usage of the marketed antibiotics the other is the evolution of novel antibiotics (8). At this point, there will be forever a vital need to discover new antimicrobial agents to control the resistance and shorten the duration of therapy. Because of the benzimidazoles similarity to purine, antibacterial ability of benzimidazoles are explained by their contest with purines resulting in growth inhibition by inhibit synthesis of bacterial nucleic acids and proteins [31]. Worldwide biochemical and pharmacological studies have proved that its derivatives are active against various strains of microorganisms [32]. For example, change the amide group to the anilide on the 2-phenyl benzimidazole produce antimicrobial activity[33].Hydrazone has antimicrobial activity and its considerable pharmacophore group. Furacilin, furazolidone and ftivazide are antibacterial drugs known to contain this group [31]. Our study Tables 2. and 3. showed that the derivatives (1) have a great activity against Vibrio cholera, the derivatives (1 and 8) have a great activity against Shigella while (1) derivative have a strongest activity against Pseudomonas aeruginosa and most of derivative (2a) have a strong activity against Vancomycin Resistant Staphylococcus aureus. 69 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 Table 2. Antibacterial activity of tested compounds against some Enterobacteriaceae and Vancomycin Resistant Staphylococcus aureus (VRSA) Test organisms Gram stain specify Compound No. Conc. (μL/ml) Zone of inhibition(mm) MIC (μL/ml) Vibrio cholerae G-ve 1 10-1-10-4 18 ( 1x10-3 ) 2a 10-1-10-4 15 (1x10-2) Shigella spp. G-ve 1 10-1-10-4 17 (1x10-3) 8 10-1-10-4 19 (1x10-2) Pseudomonas aeruginosa G-ve 1 10-1-10-4 20 (1x10-2) S. aureus (VRSA) G+ve 1 10-1-10-4 19 10 (1x10-2) 2a 10-1-10-4 20 ( 1x10-3 ) E.coli (UTEC) G-ve 1 10-1-10-4 No inhibition zone ------ It was found that the antibacterial of benzimidazole product expresses the excellent activity in gram positive bacteria with the inhibizone area around 18 mm and gives the moderate result in gram negative bacterial with the inhibizone area around 17 mm. As the result, when comparing with Gentamicin which is the antibiotic amino glycoside drug, the benzimidazole gives more excellent antibacterial than the drug. Table 3. Activty Against Enterobacteriaceae Vancomycin Resistant Staphylococcus aureus (VRSA). Test organisms Substance Zone of inhibition(mm) MIC (μl/ml) Vibrio cholerae 1 18 ( 1x10-3 ) Shigella spp. 8 19 (1x10-2) 1 20 (1x10-1) Pseudomonas aeruginosa 1 19 (1x10-2) S. aureus (VRSA) 2a 20 ( 1x10-3 ) Infectious diseases caused by fungi are called mycoses, and they are often chronic in nature. Mycotic infections may be superficial and involve only the skin (cutaneous mycoses extending into the epidermis), while others may penetrate the skin, causing subcutaneous or systemic infections. The characteristics of fungi are so unique and diverse that they are classified in their own kingdom. Unlike bacteria, fungi are eukaryotic, with rigid cell walls composed largely of chitin rather than peptidoglycan (a characteristic component of most bacterial cell walls). In addition, the fungal cell membrane contains Ergosterol rather than the cholesterol found in mammalian membranes. These structural characteristics are useful in targeting chemotherapeutic agents against fungal infections. In the present work, the results in Table 4. showed that a great activity against Aspergillus and Penicillium where the antibiotic Nystatin© have activity less than benzimidazole derivatives (3a, 5a and 8). Studies has showed that benzimidazole derivatives are specific inhibitor for microtubule assembly that act by binding to their heterodimeric subunit, the tubulin molecule. 70 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 Table 4. Challenge study between Nystatin and some chemical substances against Aspergillus and Penicillium fungi MIC (μl/ml) Zone of inhibition(mm) Conc. (μl/ml) Tested compound Test organism 100 IU 12 10-1 - 10-5 Nystatin® Aspergillus 3-1x10 14 10-1 - 10-5 3a 100 IU 11 10-1 - 10-5 Nystatin® Pencillium 3-1x10 15 10-1 - 10-5 3a 3-1x10 13 10-1 - 10-5 5a 3-1x10 3 10-1 - 10-5 8 Figure 1. FTIR spectrum of compound 1 Figure 2. 1H NMR spectrum of Compound 1. 71 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 Figure Error! No text of specified style in document.. FTIR spectrum of compound 2a. Figure 4. 1H NMR spectrum of Compound 2a 72 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 Figure 5. FTIR Spectrum of compound 2 b. Figure 6. FTIR spectrum of compound 3a. Figure 7. 1H NMR spectrum of Compound 3a. 73 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 Figure 8. FTIR Spectrum of compound 3b. Figure 9. 1H NMR spectrum of compound 3b. Figure 10. FTIR spectrum of compound 4a. 74 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 Figure 11. 1H NMR spectrum of Compound 4a. Figure 12. FTIR Spectrum of compound 4 b Figure 13. 1H NMR spectrum of compound 4b 75 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 Figure 14. FTIR spectrum of compound 5a. Figure 15. 1H NMR spectrum of Compound 5a Figure 16. FTIR spectrum of compound 5b. 76 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 Figure Error! No text of specified style in document.17. 1H NMR spectrum of Compound 5b. Figure 18. FTIR spectrum of compound 6. Figure 19. 1H NMR spectrum of Compound 6. 77 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 Figure 20. FTIR spectrum of compound 7. References 1. Wright, J. B. The Chemistry of the Benzimidazoles. Chemical Reviews. 1951, 48, 397- 541. 2. Kalidhar, U.; Kaur, A. An Overview on Some Benzimidazole and Sulfonamide Derivatives with Anti-Microbial Activity. Research Journal of Pharmaceutical, Biological and ChemicalSciences. 2011, 2, 1116-1135. 3. Shaharyar, M.; Mazumder, A. Benzimidazoles: A biologically active compounds. Arabian Journal of Chemistry. 2017, 10, S157-S173. 4. Khan, S. A. ; Nandan, A. M. 2-Substituted benzimidazoles as antiinflammatory and analgesic agents. Indian Journal of Heterocyclic Chemistry. 1997, 7, 55-58. 5. Kumar, N.; Sharma, C. S.; Ranawat, M. S.; Singh, H. P.; Chauhan, L. S.; Dashora, N. Synthesis, analgesic and anti-inflammatory activities of novel mannich bases of benzimidazoles. Journal of Pharmaceutical Investigation. 2015, 45, 65-71. 6. Ansari, K. F.; Lal, C. Synthesis and evaluation of some new benzimidazole derivatives as potential antimicrobial agents. European Journal of Medicinal Chemistry. 2009, 44, 2294-2299. 7. Kumar, B. V. S.; Vaidya, S. D.; Kumar, R. V.; Bhirud, S. B.; Mane, R. B. Synthesis and anti-bacterial activity of some novel 2-(6-fluorochroman-2-yl)-1-alkyl/acyl/aroyl- 1H-benzimidazoles. European Journal of Medicinal Chemistry. 2006, 41, 599-604. 8. Khabnadideh, S.; Rezaei, Z.; Pakshir, K.; Zomorodian, K.; Ghafari, N. Synthesis and antifungal activity of benzimidazole, benzotriazole and aminothiazole derivatives. Research in Pharmaceutical Sciences. 2012, 7, 65-72. 9. Hranjec, M.; Starčević, K.; Pavelić, S. K.; Lučin, P.; Pavelić, K.; Karminski Zamola, G. Synthesis, spectroscopic characterization and antiproliferative evaluation in vitro of novel Schiff bases related to benzimidazoles. European Journal of Medicinal Chemistry. 2011, 46, 2274-2279. 10. Gellis, A.; Kovacic, H.; Boufatah, N.; Vanelle, P. Synthesis and cytotoxicity evaluation of some benzimidazole-4,7-diones as bioreductive anticancer agents. European Journal of Medicinal Chemistry. 2008, 43, 1858-1864. 78 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 11. Suwalsky, M.; Castillo, I.; Sánchez-Eguía, B. N.; Gallardo, M. J.; Dukes, N.; Santiago-Osorio, E.; Aguiñiga, I.; Rivera-Martínez, A. R. In vitro effects of benzimidazole/thioether-copper complexes with antitumor activity on human erythrocytes. Journal of Inorganic Biochemistry. 2018, 178, 87-93. 12. Wang, X. J.; Xi, M. Y.; Fu, J. H.; Zhang, F. R.; Cheng, G. F.; Yin, D. L.; You, Q. D. Synthesis, biological evaluation and SAR studies of benzimidazole derivatives as H1- antihistamine agents. Chinese Chemical Letters. 2012, 23, 707-710. 13. Goswami, B.; Singh, A. K. Pharmacological Activities of Benzimidazole Derivatives- An Overview. International Journal of Science Innovations and Discoveries. 2012, 2, 121-136. 14. Manna, K.; Agrawal, Y. K. Microwave assisted synthesis of new indophenazine 1,3,5- trisubstruted pyrazoline derivatives of benzofuran and their antimicrobial activity. Bioorganic & Medicinal Chemistry Letters. 2009, 19, 2688-2692. 15. Walia, R.; Hedaitullah, M.; Naaz, S. F.; Iqbal, K. ; Lamba, H. S. Benzimidazole Derivatives- An Overview. International Journal Of Research In Pharmacy And Chemistry. 2011,1,565-574. 16. Balci, M. 6 - Spin Systems: Analysis of the 1H-NMR Spectra. In Basic 1H- and 13C- NMR Spectroscopy, Elsevier Science: Amsterdam. 2005, 159-197. 17. Simpson, J. H. Chapter 9 - Strategies for Assigning Resonances to Atoms within a Molecule. In Organic Structure Determination Using 2-D NMR Spectroscopy (Second Edition), Academic Press: Boston. 2012, 199-221. 18. Lomas, J. S. 1H NMR spectra of ethane-1,2-diol and other vicinal diols in benzene: GIAO/DFT shift calculations. Magnetic Resonance in Chemistry 2013, 51, 32-41. 19. 19.Simpson, J. H. Chapter 5 - Symmetry and Topicity. In Organic Structure Determination Using 2-D NMR Spectroscopy (Second Edition), Academic Press: Boston. 2012,113-121. 20. Simpson, J. H. Chapter 7 - Through-Space Effects: The Nuclear Overhauser Effect (NOE). In Organic Structure Determination Using 2-D NMR Spectroscopy (Second Edition), Academic Press: Boston. 2012, 169-184. 21. Robert M. Selverstein, F. X. W., Spectromeetric Identification Of Organic Compounds. Jon Wiley & Sons Inc. : New York. 1998, 90-91. 22. Zheng, Z. J.; Wang, D.; Xu, Z., ; Xu, L. W. Synthesis of bi- and bis-1,2,3-triazoles by copper-catalyzed Huisgen cycloaddition: A family of valuable products by click chemistry. Beilstein journal of organic chemistry. 2015, 11, 2557–2576. 23. Tiwari, V.K.; Mishra, B.B.; Mishra, K.B.; Mishra, N.; Singh, A.S. ; Chen, X. Cu- Catalyzed Click Reaction in Carbohydrate Chemistry. Chemical Reviews. 2016, 116,3086-3240. 24. Himo, F.; Lovell, T.; Hilgraf, R.; Rostovtsev, V. V.; Noodleman, L.; Sharpless, K. B.; Fokin, V. V. Copper(I)-Catalyzed Synthesis of Azoles. DFT Study Predicts Unprecedented Reactivity and Intermediates. Journal of the American Chemical Society. 2005, 127, 210-216. 25. Hoshino, M.; Katou, H.; Yamaguchi, K.I. ; Goto, Y. Dimethylsulfoxide-quenched hydrogen/deuterium exchange method to study amyloid fibril structure. Biochimica et Biophysica Acta (BBA)-Biomembranes. 2007, 1768,1886-1899. 79 Ibn Al-Haitham Jour. for Pure & Appl.Sci. IHJPAS https://doi.org/10.30526/32.1.1966 Vol. 32 (1) 2019 26. Hong, Y. S.; Hong, K. S.; Cho, J. H.; Volkov, V. I.; Lee, C. H. Chemical exchange of hydroxyl protons in quercetin measured by pulsed field gradient NMR. Applied Magnetic Resonance. 2009, 35, 261-270. 27. McFarland, L. V. Meta-analysis of probiotics for the prevention of antibiotic associated diarrhea and the treatment of Clostridium difficile disease. The American journal of gastroenterology. 2006, 101, 812-822. 28. Flórez, A. B.; Egervärn, M.; Danielsen, M.; Tosi, L.; Morelli, L.; Lindgren, S.; Mayo, B. Susceptibility of Lactobacillus plantarum strains to six antibiotics and definition of new susceptibility–resistance cutoff values. Microbial Drug Resistance. 2006, 12, 252-256. 29. Hicks, C. L.; von Baeyer, C. L.; Spafford, P. A.; van Korlaar, I.; Goodenough, B. The Faces Pain Scale–Revised: toward a common metric in pediatric pain measurement. Pain. 2001, 93, 173-183. 30. Nazzaro, F.; Fratianni, F.; De Martino, L.; Coppola, R.; De Feo, V., Effect of essential oils on pathogenic bacteria. Pharmaceuticals. 2013, 6, 1451-1474. 31. Balouiri, M.; Sadiki, M.; Ibnsouda, S. K.; Zhang, P.; Gou, Y.-Q.; Gao, X.; Bai, R.-B.; Zhao, W.-H.; Chaudhary, D. V.; Patel, D. P. Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis. 2016, 6, 71-79. 32. Arikan, S.; Yurdakul, P.; Hascelik, G., Comparison of two methods and three end points in determination of in vitro activity of micafungin against Aspergillus spp. Antimicrobial agents and chemotherapy. 2003, 47,2640-2643. 33. López-Oviedo, E.; Aller, A.; Martin, C.; Castro, C.; Ramirez, M.; Pemán, J.; Cantón, E.; Almeida, C.; Martín-Mazuelos, E., Evaluation of disk diffusion method for determining posaconazole susceptibility of filamentous fungi: comparison with CLSI broth microdilution method. Antimicrobial agents and chemotherapy. 2006, 50,1108- 1111.