Microsoft Word - 11 448 | Chemistry 2014) عام 3العدد ( 27مجلة إبن الھيثم للعلوم الصرفة و التطبيقية المجلد Ibn Al-Haitham Jour. for Pure & Appl. Sci. Vol. 27 (3) 2014 Simultaneous Determination of Ciprofloxacin Hydrochloride and Mebeverin Hydrochloride by Derivative Spectrophotometry Maha A. Mohammmed. Muna A. Kadhum Dept. of Chemistry/ College of Education for pur science/(Ibn Al-Haitham)/ University Of Baghdad Received in:28 May 2014,Accepted in:15 September 2014 Abstract A new Spectrophotometric method, is for individual and simultaneous determination of Ciprofloxacin hydrochloride(CIP) and Mebeverin hydrochloride(MEB) by the first and second derivative mode techniques. The first and second derivative spectra of these compounds permitted individual and simultaneous determination of CIP and MEB in concentration range of (4-28µg/mL) by measuring the amplitude of peak- to- base line and the area under peak at selected spectrum intervals. The methods showed a reasonable precision and accuracy and have been applied to determine CIP and MEB in four different pharmaceutical preparations. Key Words: Derivative, Ciprofloxacin hydrochloride, Mebeverin hydrochloride, Determination, Spectrophotometry 449 | Chemistry 2014) عام 3العدد ( 27مجلة إبن الھيثم للعلوم الصرفة و التطبيقية المجلد Ibn Al-Haitham Jour. for Pure & Appl. Sci. Vol. 27 (3) 2014 Introduction Ciprofloxacin hydrochloride (CIP) is a synthetic chemotherapeutic antibiotic (1- cyclopropyl-6-fluoro-1, 4-dihydro-4- oxo-7-(1-piperazinyl)-3-quinolinecarboxylic acid), Its empirical formula is C17H18FN3O3 and its molecular weight is 331.4 g/molfigure1, Ciprofloxacin hydrochloride is a broad-spectrum antimicrobial agents belonging to the fluoroquinolone group Its mode of action depends upon blocking bacterial DNA replication by binding itself to an enzyme called DNA gyrase, thereby preventing the enzyme ability to untwist the DNA double helix, which is required for DNA replication [1,2]. Mebeverine hydrochloride (MEB) has an empirical formulaC25H35NO5, chemical name. 4- (ethyl[1-(4-methoxyphenyl)propan-2-yl]amino)butyl-3,4 dimethoxybenzoate)and its molecular weight is 429,6[g/mol] as shown in figure 2. MEB is a drug whose major therapeutic role is in the treatment of irritable bowel syndrome (IBS) and the associated abdominal cramping. It works by relaxing the muscles in and around the gut. It is a musculotropic antispasmodic drug without anticholinergic side- effects. The drug is also indicated for treatment of gastrointestinal spasm secondary to organic disorder Hydrochloride [3].Most methods for CIP analysis include high performance liquid chromatographic techniques [4,5], thin layer chromatography [6,7], gas chromatography [8], capillary electrophoresis [9,10] , polarography [ 11]and spectrophotometry [12,13 ] were reported. Different methods have been reported form the determination of MEB including ,spectrophotometric methods [14-15], high performance liquid chromatographic techniques [16,17]. The main goal of this work is to establish accurate, precise, rapid and reproducible stability indicating spectrophotometric method for the determination of CIP, MEB individually. And for simultaneous determination of CIP.AndMEB. In binary mixtures, this can be used for the routine quality control analysis of these drugs in raw material and pharmaceutical formulations and stability studies. Experimental Apparatus UV-visible spectrophotometer (Shimadzu 1800) with UV-Probe Version 1.10 (Japan) connected to computer was used for the drugs estimation. Quartz cuvettes (1.00 cm) were matched and used for all absorbance measurements. Chemicals Pure gift samples of (CIP) and (MEB) double distilled water were provided by the state company of drug industries and medical appliances (IRAQ_Samara), CIP (500mg) tablet and MEB (135mg) tablet from local market. All drugs were used as working standards without further purification. Preparation of Stock and Working Standard Solutions: The stock solutions of CIP and MEB (100 µg/mL) were prepared by dissolving 10 mg of drugs in 100 mL water using volumetric flask. The working standard solutions of the respective drugs were prepared by several dilution using water . Procedure 1- Individual Determination of CIP and MEB Aliquots of CIP or MEB solution containing (4-20)µg/mL for each one aliquots alone were transfered into 10 mL volumetric flasks and dilute to mark with double distilled water. The absorption spectra were recoded and showed absorption maxima at 206,276and 316 nm for CIP, and 220, 262 and 293nm for MEB. 450 | Chemistry 2014) عام 3العدد ( 27مجلة إبن الھيثم للعلوم الصرفة و التطبيقية المجلد Ibn Al-Haitham Jour. for Pure & Appl. Sci. Vol. 27 (3) 2014 Determination was made by measuring the first and second derivative values and area under peaks of their spectra at certain given wavelengths and wavelength regions. The concentration of CIP and MEB was determined respectively. Simultaneous Determination of CIP and MEB (i) The content of series of 10 mL calibration flasks containing (8 and 14 µg/mL) for (CIP) with different concentrations (4, 8, 12, 16, and 20 µg/mL) of (MEB) were diluted with double distilled water. The absorption spectra were recorded against blank (prepared by the same manner as test solution but without CIP and MEB). The derivative values of their first andsecond spectra the concentration of CIP were masured and determined. (ii) The content of series of 10 mL calibration flasks containing (8 µg/mL) for (MEB) with different concentrations (4, 8, 10,12and 16 µg/mL) of (CIP) were diluted with double distilled water. The absorption spectra were recorded against blank (prepared by the same manner as test solution but without MEB and CIP). The derivative values of their first and second spectra were measured and the concentration of MEB was determined. 3- Standard Addition Method It was carried out by preparing several 10 mL aliquot solutions containing the same amount (40 µg) of CIP (or MEB) drug ( tablet), and different amounts of standard (0,40, 80,100,120) µg, similary for (MEB). The first and second derivative were recorded, figure 3. 4- Effect of interference This research includes, a study about the effect of different interferences on the first and second derivative of spectra for CIP and MEB which are found in pharmaceutical material A stock solution of Glucose, Lactose and Starch were prepared by dissolving (0.1g)of each additives in 10 mL volumetric flask to get a solution of 10000 µg/mL. In 10 mL calibrated flask containing (8,16) µg/mL of CIP and (12,16) µg/mL of MEB,1ml aliquots were transferred of to each additive, from which the absorption spectra were recoded. Preparation of Pharmaceutical Formulation Ten tablets were weighed and crushed to fine powder.The tablet powder equivalent to 500 mg of (CIP), 135 mg of (MEB) are mixed and dissolved in 2 mL double distilled water. The resultant solution was diluted to 100 mLwith double distilled water in volumetric flask. The solution was filtrate by using Whatman filter paper no.41to avoid any suspended or undissolved material before analyses. Results and Discussion Absorption Spectra The absorption spectra of the (CIP) and (MEB) were measured from (190-400 nm.) against double distilled water as blank. The absorption spectra of CIP and MEB and for their mixture were recorded. Fig.(4) (a) shows the absorption spectrum of CIP solution (14 µg/mL) with three absorption maxima at wavelength 206, 276 and 316 nm, while spectrum (b) shows the absorption spectrum of MEB solution (8 µg/mL) with three absorption maxima at wavelength 220,262 and 293nm .The total spectrum of mixture of ( 14 CIP /and 8 MEB µg/mL) is shown in curve( c) with (224 and 274 nm)between the absorption maxima of the two components. First and Second Derivative Modes The first and second order derivative spectra of (CIP) and (MEB) and for their mixture are shown in Figure 5 and Figure 6 respectively. It was obvious that there is a large overlap of the 451 | Chemistry 2014) عام 3العدد ( 27مجلة إبن الھيثم للعلوم الصرفة و التطبيقية المجلد Ibn Al-Haitham Jour. for Pure & Appl. Sci. Vol. 27 (3) 2014 spectra of CIP and MEB using the zero order absorption measurements. Therfore derivative spectrophotometric technique is of a particular utility in determining the concentration of single component in such mixtures with a large spectral overlapping. For this reason, derivative spectrophotometric methods have been applied .Both first and second order modes were tested and the results obtained showed that techniques could be successfully applied when the measurements are carried out under optimum concentration . The present work, graphically (peak-to-base line), technique in addition to peak area were used to deal with derivatives spectra to carry out the measurement. In the first and second derivative modes show a good proportionality to CIP and MEB concentration in their mixtures. To select the derivative order, the first, second, third and fourth derivative spectra of CIP and MEB were also studied. The study of first and second order spectra was simple and gave results of highest accuracy and detection limits. Figurure 7 and Figure 8 show sets of first order spectra of mixtures containing different amounts of each of CIP or MEB in the presence of (14µg/mL of CIP and 8µg/mL of MEB). The results in Figure 6 indicated that when the concentration of CIP is kept constant and the concentration of MEB varied, the peak area at the, intervals (207-233 nm ) and (239- 273nm) were proportional to the concentration of MEB. Moreover, the peak-to-base line at (218nm) and (287.5nm) was found to be a function of MEB concentration. The same features were found when inspecting Figure 7 for the determination of CIP. The peak areas in the wavelengths intervals of ( 220-236nm ), (244-262nm ) and (294-320nm) and the peak amplitude measured at peak-to-baseline (251)nm were in proportion to the concentration of CIP (Table 2 ). Figure 9 and Figure 10 have shown in further sets of second derivative of the same above mixtures. Applying the same mentioned techniques in measuring peak amplitudes (in millimeter) at peak-to-base line of the other compound, and peak areas at selected wavelengths intervals enable the measurement of MEB and CIP respectively (Table 2). Calibration Graph and Statistical Analysis The analysis characteristic and most statistical data for each of the proposed methods are given in Table 2. Under the optimum conditions, liner calibration graphs were obtained in the range of (4-28µg/mL) with correlation coefficient values in the range ( 0.9981-0.9999) for different techniques. Accuracy and Precision Under the optimum condition, the accuracy and precision of the proposed method (two different techniques for each of first and second order derivative modes) tested. Table 3 shows the values of relative error percent and relative standard deviation percent for two different levels of concentration of CIP and MEB. Application Two of proposed methods (namely first derivative peak–to–base line at 342 nm and second derivative peak –to- base line at 326nm ) were successfully applied for direct determination of CIP in two different drugs. The results obtained are presented in Table (4) , and are in quite agreement with the spiked values. On the other hand, CIP has also been successfully determined in two different pharmaceutical preparations by two of proposed methods. The results are shown in table (4).The standard additions method was used to determine each drug in pharmaceutical Tablets. Accuracy of the proposed method was assisted by determining CIP and MEB solutions using the standard additions method for the above methods and the data obtained for pharmaceutical tablets were listed in Table (4). 452 | Chemistry 2014) عام 3العدد ( 27مجلة إبن الھيثم للعلوم الصرفة و التطبيقية المجلد Ibn Al-Haitham Jour. for Pure & Appl. Sci. Vol. 27 (3) 2014 References 1- Rossi, S. (2006), Australian Medicines Handbook, ISBN 0-9757919-2-3. 2-Tadros, MI, (2010), Controlled-release effervescent floating matrix tablets of ciprofloxacin hydrochloride: development, optimization and in vitro-in vivo evaluation in healthy human volunteers, Eur J Pharm Biopharm, 74, 2 , 332-9. 3-Budavari, S. (2001), The Merk Index. An Encyclopedia of Chemicals, Drugs and Biologicals 13 Ed),Merck and Co.Inc.,Whitehouse Station,NJ,1097,1946. 4- Singh, R. Maithani, M. Saraf, SK. Saraf, S. and Gupta, RC. (2009), Simultaneous estimation of ciprofloxacin hydrochloride, ofloxacin, tinidazole and ornidazole by reverse phase – High performance liquid chromatography, EJAC, 4, 2, 161-168. 5- Imre, S. Dogaru, MT. Vari CE. Muntean, T. and Kelemen, L. (2003),Validation of an HPL C method for the determination of ciprofloxacin in human plasma. J Pharm Biomed Anal, 33, , 1, 125-30 6-Novakovic, J1. Nesmerak, K. Nova, H.and Filka, K. (2001), An HPTLC method for the determination and the purity control of ciprofloxacin HCl in coated tablets, J Pharm Biomed Anal., 25, 5-6, 957-64. 7- Yu-lin feng and Chuan Dong. October (2004), Simultaneous Determination of trace Ofloxacin,Ciprofloxacin and Sparfloxacin by Micelle TLC-Flourimetry, J of chromatographic Science, 42. 8- PNS Pai, Balaphanisekhar, B . Rao ,GK .and Pasha , K . (2006), Determination of methylene chloride organic volatile impurity in marketed formulations of ciprofloxacin, norfloxacin, pefloxacin and ofloxacin, . Indian ,J. Pharmaceutical Sciences, 68 , , 3,368-370 9- Adriana, F. Faria, Marcus, V. N. de Souza ,and Marcone A. L. de Oliveira, (2008),Validation of a capillary zone electrophoresis method for the determination of ciprofloxacin, gatifloxacin, moxifloxacin and ofloxacin in pharmaceutical formulations. J. Braz. Chem. Soc. 19 , 3, ISSN 0103-5053 10-. Katarzyna, Michalska, Genowefa, Pajchel,and Stefan, Tyski, (2004), Determination of ciprofloxacin and its impurities by capillary zone electrophoresis . Journal of Chromatography, 1051, 1–2, 267–272. 11- Salvi, V S*. Sathe, P A and Rege, P V. (2010), Determination of Tinidazole and Ciprofloxacin Hydrochloride in Single Formulation Tablet using Differential Pulse Polarography J Anal Bioanal Tech., 1:110. doi:10.4172/2155-9872.1000110 12- Marianne Alphonse Mahrouse , (2012), Development and validation of a UV spectrophotometric method for the simultaneous determination of ciprofloxacin hydrochloride and metronidazole in binary mixture, Journal of chemical and pharmaceutical Research, 4, , 11, 4710-4715,. 13- Manaswi Patil. Vrushali tambe. Vijaya Vichare and Reshma Kolte, (2012), Validated simultaneous UV spectrophotometric methods for estimation of ciprofloxacin and Tinidazole in tablet dosage form,. International Journal of Pharmacy and Pharmaceutical Sciences 4, 182- 185, 3. 14- Sayed, M.S.Derayea. (2014), An application of eosin y for the selective spectrophotometer and spectrofluorimetric determination of Mebeverine Hydrochloride, ,Journal Home. 6, 2270-2275. 15-. Eglal, A.Abd el aleem and Nada abdel Wahab, (2012), Simltaneous determination of some antiprotozoal drugs in different combined dosage forms by mean centering of ration spectra and multivariate calibration with model updating methods, chemistry central Journal6, 27,1, 1-8. 16- Rim, S, Haggag, Rasha, A. Shaalan and Tarek, S. Bela(2010), Validated HPLC Determination of the Two Fixed Dose Combinations (Chlordiazepoxide Hydrochloride and 453 | Chemistry 2014) عام 3العدد ( 27مجلة إبن الھيثم للعلوم الصرفة و التطبيقية المجلد Ibn Al-Haitham Jour. for Pure & Appl. Sci. Vol. 27 (3) 2014 Mebeverine Hydrochloride; Carvedilol and Hydrochlorothiazide) in Their Tablets; J. AOAC Int. 93, 4, 1192-1200. 17- Eglal A. Abd el aleem and Nada Abdel Wahab, (2013), Validated Chromatographic and spectrophotometric methods for analysis of some amoebicide drugs in their combined pharmaceutical preparation., Pak.J.Pharm.Sci. 26, 1, 175-183. Table No. (1) : Percent recovery for (8,16) µg.mL-1of CIP and (12,16)µg.mL-1of MEB in the presence of 1000 µg.mL-1of excipients. RE% Recovery conc. Found (µg/mL) conc. Taken (µg/mL) Excipients Compound -0.062 99.900 7.995 8 Lactose Ciprofloxacin hydrochloride -0.262 99.580 7.979 Starch -0.012 99.980 7.999 Glucose -0.050 99.890 15.992 16 Lactose -0.012 99.900 15.998 Starch 0.000 99.999 16.000 Glucose -0.166 98.990 11.980 12 Lactose Mebevrine hydrochloride -0.066 99.986 11.992 Starch -0.016 99.969 11.998 Glucose -0.018 99.997 15.997 16 Lactose -0.093 99.959 15.985 Starch -0.068 99.999 15.989 Glucose 454 | Chemistry 2014) عام 3العدد ( 27مجلة إبن الھيثم للعلوم الصرفة و التطبيقية المجلد Ibn Al-Haitham Jour. for Pure & Appl. Sci. Vol. 27 (3) 2014 Table No. (2) Statistical analysis of the determination of Ciprofloxacin hydrochloride and Mebeverine hydrochloride Slope Correlation coefficent (r) Regression equation Wave length (nm) Mode of calculation Order of derivative Compound 0.0026 0.9983 Y= 0.0026x + 0.0026 262 Peak to base line First Cprophloxacine Hydrochlored -0.0027 0.9984 Y=-0.0027x-0.0018 288 Peak to base line First -0.0008 0.9991 Y=-0.0008x-0.0003 342 Peak to base line First 0.0467 0.9985 Y = 0.0467x+0.044 240-274 Peak area First -0.107 0.9965 Y=-0.0401x-0.0522 276-306 Peak area First 0.0193 0.9936 Y=-0.0193x-0.0060 320-376 Peak area First 0.0002 0.9992 Y=0.0002x+ 0.0002 254 Peak to base line second 0.0004- 0.9964 Y=-0.0004X-0.0006 276 Peak to base line second 0.0002 0.9995 Y=0.0002X-0.0002 296 Peak to base line second -5E-05 0.9991 Y=-5E-05X-4E-05 326 Peak to base line second 0.0018 0.9964 Y=0.0018X+0.0005 240-262 Peak area second 0.0049- 0.9975 Y=-0.0049X-0.0072 264-286 Peak area second 0.0024 0.9984 Y=0.0024X+0.0025 288-308 Peak area second -0.0006 0.9992 Y=-0.0006X-0.0003 312-338 Peak area second -0.002 0.9994 Y=-0.002X-0.0006 230 Peak to base line First Mebeverine hydrochloride 0.0007 0.9990 Y=0.0007X+0.0004 252 Peak to base line First -0.0005 0.9990 Y=-0.0005X-2E-05 274 Peak to base line First -0.0005 0.9999 Y=-0.0005X-0.0003 306 Peak to base line First -0.014 0.9985 Y=-0.014X+0.0032 222-242 Peak area First 0.0067 0.9992 Y=0.0067X+0.0029 244-262 Peak area First -0.0041 0.9982 Y=-0.0041X-0.0049 264-286 Peak area First -0.729 0.9995 Y=-0.0059X-0.0033 292-324 Peak area First 0.0003 0.9993 Y=0.0003X+4E-05 238 Peak to base line second -0.0001 0.9995 Y=-0.0001X-0.0002 262 Peak to base line second 5E-05 0.9994 Y=5E-05X - 1E-05 280 Peak to base line second -4E-05 0.9997 Y=-4E-05X – 7E-06 296 Peak to base line second 4E-05 0.9991 Y=4E-05X + 2E-05 314 Peak to base line second 0.0023 0.9991 Y= 0.0023X + 4E-05 230-248 Peak area second -0.0008 0.9989 Y=-0.0008X -0.0001 252-270 Peak area second 0.0002 0.9996 Y=0.0002X+0.0001 274-286 Peak area second -0.0004 0.9995 Y= -0.0004X-9E-05 288-304 Peak area second 0.0006 0.9993 Y=0.0006X+0.0003 304-332 Peak area second 455 | Chemistry 2014) عام 3العدد ( 27مجلة إبن الھيثم للعلوم الصرفة و التطبيقية المجلد Ibn Al-Haitham Jour. for Pure & Appl. Sci. Vol. 27 (3) 2014 Table No. (3) Precision and accuracy of the methods RSD % RE % Found (µg/mL) Taken (µg/mL) Method of analysis Compound 0.436 0.750- 3.970 4 First order peak-to-base line at 342 nm 1.540 2.741 12.329 12 0.378 0.660 28.185 28 2.762 5.025 4.201 4 Second order peak-to- base line at 326nm 1.458 2.591 12.311 12 0.254 0.442 28.124 28 1.979 3.550 6.213 6 First order peak-to-base line at 306 nm 0.009 0.016 - 11.998 12 0.030 0.053 28.015 28 0.183 -0.317 5.981 6 Second order peak-to- base line at 296 nm 1.048 1.850 12.222 12 0.285 0.496 28.139 28 *Average of four determination Table No. (4) Results for analysis of Ciprofloxacin hydrochloride and Mebeverine hydrochloride in two pharmaceutical formulation sample RSD % n =3 RE% r Regression equation Wave length ( nm) Found (µg/mL) Taken (µg/mL) Method of analysis Compound 1.892     2.75  0.9993 Y=-0.0084X-0.0034 342 4.11 4 First Ciprofloxacin hydrochloride Ras Al khaimah,U.A.E. 500 mg 1.724     2.50  0.9987 Y=-0.0005X-0.0002 326 4.10 4 Second 1.627     ‐2.25   0.9999 Y= -0.0051X+0.002 306 3.91 4 First Mebeverine hydrochloride Jeddah, Saudi Arabia 135mg    2.564    ‐3.50  0.9956 Y= -0.0004X-0.0001 296 3.86 4 Second Ciprofloxacin hydrochloride Mebeverine hydrochloride 456 | Chemistry 2014) عام 3العدد ( 27مجلة إبن الھيثم للعلوم الصرفة و التطبيقية المجلد Ibn Al-Haitham Jour. for Pure & Appl. Sci. Vol. 27 (3) 2014 Figure No. (1) Chemical Formula of Ciprofloxacin Hydrochloride 457 | Chemistry 2014) عام 3العدد ( 27مجلة إبن الھيثم للعلوم الصرفة و التطبيقية المجلد Ibn Al-Haitham Jour. for Pure & Appl. Sci. Vol. 27 (3) 2014 Figure No. (2):Formula of Mebeverine hydrochloride Figure No. (3) Determination of CIP in several amount of MEB(a,b) and determination of MEB in several concentration of CIP (c,d) by standard additions method. Figure No. (4) Absorption spectra of (a) 14 µg/mL Ciprofloxacin hydrochloride , (b) 8 µg/mL Mebeverine hydrochloride (c) Ciprofloxacin hydrochloride and Mebeverine hydrochloride 458 | Chemistry 2014) عام 3العدد ( 27مجلة إبن الھيثم للعلوم الصرفة و التطبيقية المجلد Ibn Al-Haitham Jour. for Pure & Appl. Sci. Vol. 27 (3) 2014 Figure No. (5) first derivatives spectra of: (a) 14 µg/mL Ciprofloxacin hydrochloride (b) 8µg/mL Mebeverine hydrochloride (c) Ciprofloxacin hydrochloride and Mebeverine hydrochloride mixture Figure No. (6) Second derivatives spectra of: (a) 14 µg/mL Ciprofloxacin hydrochloride, (b) 8 µg/mL Mebeverine hydrochloride (c) Ciprofloxacin hydrochloride and Mebeverine hydrochlorid 459 | Chemistry 2014) عام 3العدد ( 27مجلة إبن الھيثم للعلوم الصرفة و التطبيقية المجلد Ibn Al-Haitham Jour. for Pure & Appl. Sci. Vol. 27 (3) 2014 Figure No.(7): First derivative spectra of mixtures containing(4-20 µg/mL) Mebeverinehydrochloride and 14 µg/mL of Ciprofloxacin hydrochloride Figure No. (8) :First derivative spectra of mixtures containing(4-20 µg/mL) Ciprofloxacin hydrochloride and 14 µg/mL of Mebeverinehydrochloride 460 | Chemistry 2014) عام 3العدد ( 27مجلة إبن الھيثم للعلوم الصرفة و التطبيقية المجلد Ibn Al-Haitham Jour. for Pure & Appl. Sci. Vol. 27 (3) 2014 Figure No. (9): Second derivative spectra of mixtures containing of Mebeverine hydrochloride (4-20 µg/mL) and Ciprofloxacin hydrochloride14 µg/mL \ Figure No. (10): Second derivative spectra of mixtures containingof (4-20 µg/mL) Ciprofloxacin hydrochloride and Mebeverine hydrochloride 8 µg/mL 461 | Chemistry 2014) عام 3العدد ( 27مجلة إبن الھيثم للعلوم الصرفة و التطبيقية المجلد Ibn Al-Haitham Jour. for Pure & Appl. Sci. Vol. 27 (3) 2014 التقدير االني لعقاري السبروفلوكساسين ھايدروكلوريد و المبفرين ھايدروكلوريد بوساطة طيف المشتقة مھا عبد الستار محمد نجيب منى عبد الرسول كاظم للعلوم الصرفة(ابن الھيثم)/جامعة بغدادقسم الكيمياء/كلية التربية 2014ايلول15,قبل البحث في:2014ايار28استلم البحث في: الخالصة نفرد وبشكل اني طورت طريقة طيفية جديدة لتقدير السبروفلوكساسين ھيدروكلوريد والمبيفرين ھيدروكلوريد بشكل م لمشتقة الثانية الطياف ھذه المركبات. لقد وجد ان المشتقة االولى واباالعتماد على تقنية المشتقة االولى والمشتقة الثانية د بمدى يتراوح بين الطياف ھذه المركبات تمكن من التقديراالني للسبروفلوكساسين ھيدروكلوريد وللمبيفرين ھيدروكلوري وال حة تحت الحزمة عند اطخط القاعدة وكذلك من خالل قياس المسا -مل وذلك بقياس ارتفاع القمة\) مايكروغرام 28 - 4( دقيقة بشكل موجية محددة لكل مركب. لقد كانت النتائج التي تم الحصول عليھامن تحليل المركبات قيد الدراسة متوافقة و اجح في اثنين من مقبول, كما وامكن تطبيقھا لتقدير السبروفلوكساسين ھيدروكلوريد والمبيفرين ھيدروكلوريد بشكل ن لصيدالنيةا المستحضرات يافتقدير , المط المشتقة , سبروفلوكساسين ھايدروكلوريد, مبفرين ھايدروكلوريد ,: الكلمات المفتاحية