id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
iajs-1062	Mohammad, Taghreed A.; Mahdi, Sawsan Abdul Hussain	Isolation and Partial Purification of Cell wall Lipopolysaccharides of Pseudomonas Aeruginosa and Using It as Vaccine	2017	8	.pdf	application/pdf	2321	132	59	Vol. 30 (1) 2017 Table (2) Determination of LD50 of LPS purified from P. aeruginosa ATCC 15692 and its mortality rate Table (3) Immunization of LPS as a vaccine in mice body Figure (1) Gel filtration chromatography of LPS produced from P. aeruginosa ATCC 15692 Groups Type LPS injected subcutaneously Prednisolone Infections A Control 11.905 μg/ mouse 0 B Vaccination 11.905 μg/mouse 25μg/dl 6 C Control 6 LPS Dose/moue Number of Animals Mortality Rate Chromatography technique was employed to extract and purify cell wall containing lipopolysaccharide by using P. aeruginosa isolate ATCC 15692,the purification done by addition of ammonuium sulfate, sodium dodecyl sulfat (SDS) anddialysis, gel filtration chromatography by using sepharose-4B. Immunogenicity of LPS component was determined by mice injection under the skin ,then Ab concentration against LPS component formed and determined by ELISA .	cache/iajs-1062.pdf	txt/iajs-1062.txt