id	sid	tid	token	lemma	pos
iajs-2699	1	1	35	35	NUM
iajs-2699	1	2	polymer	polymer	NOUN
iajs-2699	1	3	chain	chain	NOUN
iajs-2699	1	4	reaction	reaction	NOUN
iajs-2699	1	5	(	(	PUNCT
iajs-2699	1	6	pcr	pcr	ADJ
iajs-2699	1	7	):	):	PUNCT
iajs-2699	1	8	principle	principle	NOUN
iajs-2699	1	9	and	and	CCONJ
iajs-2699	1	10	applications	application	NOUN
iajs-2699	1	11	noorhan	noorhan	ADP
iajs-2699	1	12	khalid	khalid	PROPN
iajs-2699	1	13	shafeeq	shafeeq	PROPN
iajs-2699	1	14	department	department	PROPN
iajs-2699	1	15	of	of	ADP
iajs-2699	1	16	chemistry/	chemistry/	NUM
iajs-2699	1	17	college	college	NOUN
iajs-2699	1	18	of	of	ADP
iajs-2699	1	19	education	education	NOUN
iajs-2699	1	20	,	,	PUNCT
iajs-2699	1	21	for	for	ADP
iajs-2699	1	22	pure	pure	ADJ
iajs-2699	1	23	science	science	NOUN
iajs-2699	1	24	(	(	PUNCT
iajs-2699	1	25	ibn	ibn	PROPN
iajs-2699	1	26	-	-	PUNCT
iajs-2699	1	27	al	al	PROPN
iajs-2699	1	28	-	-	PUNCT
iajs-2699	1	29	haitham	haitham	PROPN
iajs-2699	1	30	)	)	PUNCT
iajs-2699	1	31	university	university	PROPN
iajs-2699	1	32	of	of	ADP
iajs-2699	1	33	baghdad	baghdad	PROPN
iajs-2699	1	34	.	.	PUNCT
iajs-2699	2	1	iraq	iraq	PROPN
iajs-2699	2	2	.	.	PUNCT
iajs-2699	3	1	noorhan.albayaty@gmail.com	noorhan.albayaty@gmail.com	X
iajs-2699	3	2	abstract	abstract	ADJ
iajs-2699	3	3	the	the	DET
iajs-2699	3	4	new	new	ADJ
iajs-2699	3	5	,	,	PUNCT
iajs-2699	3	6	standard	standard	ADJ
iajs-2699	3	7	molecular	molecular	ADJ
iajs-2699	3	8	biologic	biologic	NOUN
iajs-2699	3	9	system	system	NOUN
iajs-2699	3	10	for	for	ADP
iajs-2699	3	11	duplicating	duplicate	VERB
iajs-2699	3	12	dna	dna	NOUN
iajs-2699	3	13	enzymatically	enzymatically	ADV
iajs-2699	3	14	devoid	devoid	ADJ
iajs-2699	3	15	of	of	ADP
iajs-2699	3	16	employing	employ	VERB
iajs-2699	3	17	a	a	DET
iajs-2699	3	18	living	live	VERB
iajs-2699	3	19	organism	organism	NOUN
iajs-2699	3	20	,	,	PUNCT
iajs-2699	3	21	like	like	ADP
iajs-2699	3	22	e.	e.	PROPN
iajs-2699	3	23	coli	coli	PROPN
iajs-2699	3	24	or	or	CCONJ
iajs-2699	3	25	yeast	yeast	NOUN
iajs-2699	3	26	,	,	PUNCT
iajs-2699	3	27	represents	represent	VERB
iajs-2699	3	28	polymerases	polymerase	NOUN
iajs-2699	3	29	chain	chain	NOUN
iajs-2699	3	30	reaction	reaction	NOUN
iajs-2699	3	31	(	(	PUNCT
iajs-2699	3	32	pcr	pcr	PROPN
iajs-2699	3	33	)	)	PUNCT
iajs-2699	3	34	.	.	PUNCT
iajs-2699	4	1	this	this	DET
iajs-2699	4	2	technology	technology	NOUN
iajs-2699	4	3	allows	allow	VERB
iajs-2699	4	4	an	an	DET
iajs-2699	4	5	exponential	exponential	ADJ
iajs-2699	4	6	intensification	intensification	NOUN
iajs-2699	4	7	of	of	ADP
iajs-2699	4	8	a	a	DET
iajs-2699	4	9	minor	minor	ADJ
iajs-2699	4	10	quantity	quantity	NOUN
iajs-2699	4	11	of	of	ADP
iajs-2699	4	12	dna	dna	PROPN
iajs-2699	4	13	molecule	molecule	NOUN
iajs-2699	4	14	several	several	ADJ
iajs-2699	4	15	times	time	NOUN
iajs-2699	4	16	.	.	PUNCT
iajs-2699	5	1	analysis	analysis	NOUN
iajs-2699	5	2	can	can	AUX
iajs-2699	5	3	be	be	AUX
iajs-2699	5	4	straightforward	straightforward	ADJ
iajs-2699	5	5	with	with	ADP
iajs-2699	5	6	more	more	ADJ
iajs-2699	5	7	dna	dna	NOUN
iajs-2699	5	8	available	available	ADJ
iajs-2699	5	9	.	.	PUNCT
iajs-2699	6	1	a	a	DET
iajs-2699	6	2	thermal	thermal	ADJ
iajs-2699	6	3	heat	heat	NOUN
iajs-2699	6	4	cycler	cycler	NOUN
iajs-2699	6	5	performs	perform	VERB
iajs-2699	6	6	a	a	DET
iajs-2699	6	7	polymerization	polymerization	NOUN
iajs-2699	6	8	chain	chain	NOUN
iajs-2699	6	9	reaction	reaction	NOUN
iajs-2699	6	10	that	that	PRON
iajs-2699	6	11	involves	involve	VERB
iajs-2699	6	12	repeated	repeat	VERB
iajs-2699	6	13	cycles	cycle	NOUN
iajs-2699	6	14	of	of	ADP
iajs-2699	6	15	heating	heating	NOUN
iajs-2699	6	16	and	and	CCONJ
iajs-2699	6	17	cooling	cool	VERB
iajs-2699	6	18	the	the	DET
iajs-2699	6	19	reactant	reactant	NOUN
iajs-2699	6	20	tubes	tube	NOUN
iajs-2699	6	21	at	at	ADP
iajs-2699	6	22	the	the	DET
iajs-2699	6	23	desired	desire	VERB
iajs-2699	6	24	temperature	temperature	NOUN
iajs-2699	6	25	for	for	ADP
iajs-2699	6	26	each	each	DET
iajs-2699	6	27	reaction	reaction	NOUN
iajs-2699	6	28	step	step	NOUN
iajs-2699	6	29	.	.	PUNCT
iajs-2699	7	1	a	a	DET
iajs-2699	7	2	heated	heated	ADJ
iajs-2699	7	3	deck	deck	NOUN
iajs-2699	7	4	is	be	AUX
iajs-2699	7	5	positioned	position	VERB
iajs-2699	7	6	on	on	ADP
iajs-2699	7	7	the	the	DET
iajs-2699	7	8	upper	upper	ADJ
iajs-2699	7	9	reaction	reaction	NOUN
iajs-2699	7	10	tube	tube	NOUN
iajs-2699	7	11	to	to	PART
iajs-2699	7	12	avoid	avoid	VERB
iajs-2699	7	13	evaporating	evaporate	VERB
iajs-2699	7	14	the	the	DET
iajs-2699	7	15	reaction	reaction	NOUN
iajs-2699	7	16	mixture	mixture	NOUN
iajs-2699	7	17	(	(	PUNCT
iajs-2699	7	18	normally	normally	ADV
iajs-2699	7	19	volumes	volume	NOUN
iajs-2699	7	20	range	range	VERB
iajs-2699	7	21	from	from	ADP
iajs-2699	7	22	15	15	NUM
iajs-2699	7	23	to	to	PART
iajs-2699	7	24	100	100	NUM
iajs-2699	7	25	l	l	NOUN
iajs-2699	7	26	per	per	ADP
iajs-2699	7	27	tube	tube	NOUN
iajs-2699	7	28	)	)	PUNCT
iajs-2699	7	29	,	,	PUNCT
iajs-2699	7	30	or	or	CCONJ
iajs-2699	7	31	an	an	DET
iajs-2699	7	32	oil	oil	NOUN
iajs-2699	7	33	layer	layer	NOUN
iajs-2699	7	34	can	can	AUX
iajs-2699	7	35	be	be	AUX
iajs-2699	7	36	placed	place	VERB
iajs-2699	7	37	on	on	ADP
iajs-2699	7	38	a	a	DET
iajs-2699	7	39	reaction	reaction	NOUN
iajs-2699	7	40	mixture	mixture	NOUN
iajs-2699	7	41	surface	surface	NOUN
iajs-2699	7	42	.	.	PUNCT
iajs-2699	8	1	the	the	DET
iajs-2699	8	2	amplified	amplify	VERB
iajs-2699	8	3	dna	dna	PROPN
iajs-2699	8	4	fragment	fragment	NOUN
iajs-2699	8	5	is	be	AUX
iajs-2699	8	6	determined	determine	VERB
iajs-2699	8	7	based	base	VERB
iajs-2699	8	8	on	on	ADP
iajs-2699	8	9	selecting	select	VERB
iajs-2699	8	10	primers	primer	NOUN
iajs-2699	8	11	in	in	ADP
iajs-2699	8	12	addition	addition	NOUN
iajs-2699	8	13	to	to	ADP
iajs-2699	8	14	the	the	DET
iajs-2699	8	15	starting	starting	NOUN
iajs-2699	8	16	and	and	CCONJ
iajs-2699	8	17	end	end	NOUN
iajs-2699	8	18	of	of	ADP
iajs-2699	8	19	the	the	DET
iajs-2699	8	20	dna	dna	PROPN
iajs-2699	8	21	fragment	fragment	NOUN
iajs-2699	8	22	.	.	PUNCT
iajs-2699	9	1	the	the	DET
iajs-2699	9	2	primers	primer	NOUN
iajs-2699	9	3	stand	stand	VERB
iajs-2699	9	4	for	for	ADP
iajs-2699	9	5	short	short	ADJ
iajs-2699	9	6	,	,	PUNCT
iajs-2699	9	7	artificial	artificial	ADJ
iajs-2699	9	8	dna	dna	NOUN
iajs-2699	9	9	stripes	stripe	NOUN
iajs-2699	9	10	,	,	PUNCT
iajs-2699	9	11	no	no	ADV
iajs-2699	9	12	higher	high	ADJ
iajs-2699	9	13	than	than	ADP
iajs-2699	9	14	fifty	fifty	NUM
iajs-2699	9	15	(	(	PUNCT
iajs-2699	9	16	typically	typically	ADV
iajs-2699	9	17	18	18	NUM
iajs-2699	9	18	-	-	PUNCT
iajs-2699	9	19	25bp	25bp	NOUN
iajs-2699	9	20	)	)	PUNCT
iajs-2699	9	21	nucleotides	nucleotide	NOUN
iajs-2699	9	22	have	have	AUX
iajs-2699	9	23	been	be	AUX
iajs-2699	9	24	based	base	VERB
iajs-2699	9	25	on	on	ADP
iajs-2699	9	26	a	a	DET
iajs-2699	9	27	starting	starting	NOUN
iajs-2699	9	28	and	and	CCONJ
iajs-2699	9	29	ending	ending	NOUN
iajs-2699	9	30	of	of	ADP
iajs-2699	9	31	dna	dna	PROPN
iajs-2699	9	32	fragment	fragment	NOUN
iajs-2699	9	33	to	to	PART
iajs-2699	9	34	be	be	AUX
iajs-2699	9	35	amplified	amplify	VERB
iajs-2699	9	36	.	.	PUNCT
iajs-2699	10	1	dna	dna	NOUN
iajs-2699	10	2	-	-	PUNCT
iajs-2699	10	3	polymerase	polymerase	NOUN
iajs-2699	10	4	connects	connect	VERB
iajs-2699	10	5	and	and	CCONJ
iajs-2699	10	6	starts	start	VERB
iajs-2699	10	7	a	a	DET
iajs-2699	10	8	new	new	ADJ
iajs-2699	10	9	dna	dna	NOUN
iajs-2699	10	10	strand	strand	NOUN
iajs-2699	10	11	synthesis	synthesis	NOUN
iajs-2699	10	12	the	the	DET
iajs-2699	10	13	pcr	pcr	ADJ
iajs-2699	10	14	products	product	NOUN
iajs-2699	10	15	can	can	AUX
iajs-2699	10	16	be	be	AUX
iajs-2699	10	17	visualized	visualize	VERB
iajs-2699	10	18	by	by	ADP
iajs-2699	10	19	dual	dual	ADJ
iajs-2699	10	20	foremost	foremost	ADJ
iajs-2699	10	21	methods	method	NOUN
iajs-2699	10	22	:	:	PUNCT
iajs-2699	10	23	(	(	PUNCT
iajs-2699	10	24	1	1	X
iajs-2699	10	25	)	)	PUNCT
iajs-2699	10	26	staining	stain	VERB
iajs-2699	10	27	of	of	ADP
iajs-2699	10	28	the	the	DET
iajs-2699	10	29	product	product	NOUN
iajs-2699	10	30	of	of	ADP
iajs-2699	10	31	dna	dna	PROPN
iajs-2699	10	32	amplified	amplify	VERB
iajs-2699	10	33	by	by	ADP
iajs-2699	10	34	a	a	DET
iajs-2699	10	35	chemical	chemical	ADJ
iajs-2699	10	36	dye	dye	NOUN
iajs-2699	10	37	like	like	ADP
iajs-2699	10	38	bromide	bromide	NOUN
iajs-2699	10	39	ethidium	ethidium	NOUN
iajs-2699	10	40	,	,	PUNCT
iajs-2699	10	41	or	or	CCONJ
iajs-2699	10	42	(	(	PUNCT
iajs-2699	10	43	2	2	X
iajs-2699	10	44	)	)	PUNCT
iajs-2699	10	45	marking	marking	NOUN
iajs-2699	10	46	of	of	ADP
iajs-2699	10	47	fluorescent	fluorescent	ADJ
iajs-2699	10	48	dyes	dye	NOUN
iajs-2699	10	49	(	(	PUNCT
iajs-2699	10	50	fluorophores	fluorophore	NOUN
iajs-2699	10	51	)	)	PUNCT
iajs-2699	10	52	pcr	pcr	ADJ
iajs-2699	10	53	primers	primer	NOUN
iajs-2699	10	54	or	or	CCONJ
iajs-2699	10	55	nucleotides	nucleotide	NOUN
iajs-2699	10	56	before	before	ADP
iajs-2699	10	57	amplification	amplification	NOUN
iajs-2699	10	58	of	of	ADP
iajs-2699	10	59	pcrs	pcrs	PROPN
iajs-2699	10	60	.	.	PUNCT
iajs-2699	11	1	pcr	pcr	PROPN
iajs-2699	11	2	offers	offer	VERB
iajs-2699	11	3	some	some	DET
iajs-2699	11	4	benefits	benefit	NOUN
iajs-2699	11	5	.	.	PUNCT
iajs-2699	12	1	first	first	ADV
iajs-2699	12	2	,	,	PUNCT
iajs-2699	12	3	it	it	PRON
iajs-2699	12	4	is	be	AUX
iajs-2699	12	5	a	a	DET
iajs-2699	12	6	simple	simple	ADJ
iajs-2699	12	7	method	method	NOUN
iajs-2699	12	8	of	of	ADP
iajs-2699	12	9	understanding	understanding	NOUN
iajs-2699	12	10	and	and	CCONJ
iajs-2699	12	11	using	use	VERB
iajs-2699	12	12	and	and	CCONJ
iajs-2699	12	13	quick	quick	ADJ
iajs-2699	12	14	results	result	NOUN
iajs-2699	12	15	.	.	PUNCT
iajs-2699	13	1	it	it	PRON
iajs-2699	13	2	has	have	VERB
iajs-2699	13	3	an	an	DET
iajs-2699	13	4	extremely	extremely	ADV
iajs-2699	13	5	sensitive	sensitive	ADJ
iajs-2699	13	6	technology	technology	NOUN
iajs-2699	13	7	with	with	ADP
iajs-2699	13	8	the	the	DET
iajs-2699	13	9	potential	potential	NOUN
iajs-2699	13	10	for	for	ADP
iajs-2699	13	11	sequencing	sequence	VERB
iajs-2699	13	12	,	,	PUNCT
iajs-2699	13	13	cloning	cloning	NOUN
iajs-2699	13	14	,	,	PUNCT
iajs-2699	13	15	and	and	CCONJ
iajs-2699	13	16	analyzing	analyze	VERB
iajs-2699	13	17	millions	million	NOUN
iajs-2699	13	18	or	or	CCONJ
iajs-2699	13	19	milliards	milliard	NOUN
iajs-2699	13	20	of	of	ADP
iajs-2699	13	21	copies	copy	NOUN
iajs-2699	13	22	of	of	ADP
iajs-2699	13	23	a	a	DET
iajs-2699	13	24	particular	particular	ADJ
iajs-2699	13	25	product	product	NOUN
iajs-2699	13	26	.	.	PUNCT
iajs-2699	14	1	keywords	keyword	NOUN
iajs-2699	14	2	:	:	PUNCT
iajs-2699	14	3	pcr	pcr	ADJ
iajs-2699	14	4	,	,	PUNCT
iajs-2699	14	5	molecular	molecular	ADJ
iajs-2699	14	6	techniques	technique	NOUN
iajs-2699	14	7	.	.	PUNCT
iajs-2699	15	1	ibn	ibn	PROPN
iajs-2699	15	2	al	al	PROPN
iajs-2699	15	3	haitham	haitham	PROPN
iajs-2699	15	4	journal	journal	PROPN
iajs-2699	15	5	for	for	ADP
iajs-2699	15	6	pure	pure	ADJ
iajs-2699	15	7	and	and	CCONJ
iajs-2699	15	8	applied	apply	VERB
iajs-2699	15	9	science	science	NOUN
iajs-2699	15	10	journal	journal	PROPN
iajs-2699	15	11	homepage	homepage	NOUN
iajs-2699	15	12	:	:	PUNCT
iajs-2699	15	13	http://jih.uobaghdad.edu.iq/index.php/j/index	http://jih.uobaghdad.edu.iq/index.php/j/index	NOUN
iajs-2699	15	14	doi	doi	NOUN
iajs-2699	15	15	:	:	PUNCT
iajs-2699	15	16	10.30526/34.4.2699	10.30526/34.4.2699	NUM
iajs-2699	15	17	article	article	NOUN
iajs-2699	15	18	history	history	NOUN
iajs-2699	15	19	:	:	PUNCT
iajs-2699	15	20	received	receive	VERB
iajs-2699	15	21	25	25	NUM
iajs-2699	15	22	,	,	PUNCT
iajs-2699	15	23	july	july	PROPN
iajs-2699	15	24	,	,	PUNCT
iajs-2699	15	25	2021	2021	NUM
iajs-2699	15	26	,	,	PUNCT
iajs-2699	15	27	accepted	accept	VERB
iajs-2699	15	28	26,september	26,september	NUM
iajs-2699	15	29	,	,	PUNCT
iajs-2699	15	30	2021	2021	NUM
iajs-2699	15	31	,	,	PUNCT
iajs-2699	15	32	published	publish	VERB
iajs-2699	15	33	in	in	ADP
iajs-2699	15	34	2021	2021	NUM
iajs-2699	15	35	.	.	PUNCT
iajs-2699	16	1	mailto:.albayaty@gmail.com	mailto:.albayaty@gmail.com	X
iajs-2699	16	2	mailto:.albayaty@gmail.com	mailto:.albayaty@gmail.com	X
iajs-2699	16	3	ibn	ibn	PROPN
iajs-2699	16	4	al	al	PROPN
iajs-2699	16	5	-	-	PUNCT
iajs-2699	16	6	haitham	haitham	PROPN
iajs-2699	16	7	jour	jour	X
iajs-2699	16	8	.	.	PROPN
iajs-2699	17	1	for	for	ADP
iajs-2699	17	2	pure	pure	ADJ
iajs-2699	17	3	&	&	CCONJ
iajs-2699	17	4	appl	appl	PROPN
iajs-2699	17	5	.	.	PUNCT
iajs-2699	18	1	sci	sci	PROPN
iajs-2699	18	2	.	.	PROPN
iajs-2699	19	1	34(4)2021	34(4)2021	NUM
iajs-2699	19	2	36	36	NUM
iajs-2699	19	3	1.introduction	1.introduction	NUM
iajs-2699	19	4	the	the	DET
iajs-2699	19	5	new	new	ADJ
iajs-2699	19	6	,	,	PUNCT
iajs-2699	19	7	standard	standard	ADJ
iajs-2699	19	8	molecular	molecular	ADJ
iajs-2699	19	9	biologic	biologic	NOUN
iajs-2699	19	10	system	system	NOUN
iajs-2699	19	11	for	for	ADP
iajs-2699	19	12	duplicating	duplicate	VERB
iajs-2699	19	13	dna	dna	NOUN
iajs-2699	19	14	enzymatically	enzymatically	ADV
iajs-2699	19	15	devoid	devoid	ADJ
iajs-2699	19	16	of	of	ADP
iajs-2699	19	17	employing	employ	VERB
iajs-2699	19	18	a	a	DET
iajs-2699	19	19	living	live	VERB
iajs-2699	19	20	organism	organism	NOUN
iajs-2699	19	21	,	,	PUNCT
iajs-2699	19	22	like	like	ADP
iajs-2699	19	23	e.	e.	PROPN
iajs-2699	19	24	coli	coli	PROPN
iajs-2699	19	25	or	or	CCONJ
iajs-2699	19	26	yeast	yeast	NOUN
iajs-2699	19	27	,	,	PUNCT
iajs-2699	19	28	represents	represent	VERB
iajs-2699	19	29	polymerases	polymerase	NOUN
iajs-2699	19	30	chain	chain	NOUN
iajs-2699	19	31	reaction	reaction	NOUN
iajs-2699	19	32	(	(	PUNCT
iajs-2699	19	33	pcr	pcr	PROPN
iajs-2699	19	34	)	)	PUNCT
iajs-2699	19	35	.	.	PUNCT
iajs-2699	20	1	this	this	DET
iajs-2699	20	2	technology	technology	NOUN
iajs-2699	20	3	allows	allow	VERB
iajs-2699	20	4	an	an	DET
iajs-2699	20	5	exponential	exponential	ADJ
iajs-2699	20	6	intensification	intensification	NOUN
iajs-2699	20	7	of	of	ADP
iajs-2699	20	8	a	a	DET
iajs-2699	20	9	minor	minor	ADJ
iajs-2699	20	10	quantity	quantity	NOUN
iajs-2699	20	11	of	of	ADP
iajs-2699	20	12	dna	dna	PROPN
iajs-2699	20	13	molecule	molecule	NOUN
iajs-2699	20	14	several	several	ADJ
iajs-2699	20	15	times	time	NOUN
iajs-2699	20	16	.	.	PUNCT
iajs-2699	21	1	analysis	analysis	NOUN
iajs-2699	21	2	can	can	AUX
iajs-2699	21	3	be	be	AUX
iajs-2699	21	4	straightforward	straightforward	ADJ
iajs-2699	21	5	with	with	ADP
iajs-2699	21	6	more	more	ADJ
iajs-2699	21	7	dna	dna	NOUN
iajs-2699	21	8	available	available	ADJ
iajs-2699	21	9	.	.	PUNCT
iajs-2699	22	1	for	for	ADP
iajs-2699	22	2	many	many	ADJ
iajs-2699	22	3	tasks	task	NOUN
iajs-2699	22	4	,	,	PUNCT
iajs-2699	22	5	for	for	ADP
iajs-2699	22	6	example	example	NOUN
iajs-2699	22	7	,	,	PUNCT
iajs-2699	22	8	detecting	detect	VERB
iajs-2699	22	9	hereditary	hereditary	ADJ
iajs-2699	22	10	disease	disease	NOUN
iajs-2699	22	11	,	,	PUNCT
iajs-2699	22	12	genetic	genetic	ADJ
iajs-2699	22	13	fingerprint	fingerprint	NOUN
iajs-2699	22	14	identification	identification	NOUN
iajs-2699	22	15	,	,	PUNCT
iajs-2699	22	16	gene	gene	NOUN
iajs-2699	22	17	cloning	cloning	NOUN
iajs-2699	22	18	,	,	PUNCT
iajs-2699	22	19	genetic	genetic	ADJ
iajs-2699	22	20	testing	testing	NOUN
iajs-2699	22	21	,	,	PUNCT
iajs-2699	22	22	and	and	CCONJ
iajs-2699	22	23	dna	dna	PROPN
iajs-2699	22	24	computing	computing	NOUN
iajs-2699	22	25	,	,	PUNCT
iajs-2699	22	26	many	many	ADJ
iajs-2699	22	27	pcr	pcr	ADJ
iajs-2699	22	28	laboratories	laboratory	NOUN
iajs-2699	22	29	are	be	AUX
iajs-2699	22	30	commonly	commonly	ADV
iajs-2699	22	31	utilized	utilize	VERB
iajs-2699	22	32	in	in	ADP
iajs-2699	22	33	medical	medical	ADJ
iajs-2699	22	34	and	and	CCONJ
iajs-2699	22	35	biological	biological	ADJ
iajs-2699	22	36	research	research	NOUN
iajs-2699	22	37	[	[	X
iajs-2699	22	38	1	1	NUM
iajs-2699	22	39	]	]	PUNCT
iajs-2699	22	40	.	.	PUNCT
iajs-2699	23	1	the	the	DET
iajs-2699	23	2	technique	technique	NOUN
iajs-2699	23	3	was	be	AUX
iajs-2699	23	4	initiated	initiate	VERB
iajs-2699	23	5	by	by	ADP
iajs-2699	23	6	kary	kary	PROPN
iajs-2699	23	7	mullis	mullis	PROPN
iajs-2699	23	8	in	in	ADP
iajs-2699	23	9	1983	1983	NUM
iajs-2699	23	10	.	.	PUNCT
iajs-2699	24	1	pcr	pcr	PROPN
iajs-2699	24	2	has	have	AUX
iajs-2699	24	3	been	be	AUX
iajs-2699	24	4	nowadays	nowadays	ADV
iajs-2699	24	5	an	an	DET
iajs-2699	24	6	important	important	ADJ
iajs-2699	24	7	and	and	CCONJ
iajs-2699	24	8	typical	typical	ADJ
iajs-2699	24	9	method	method	NOUN
iajs-2699	24	10	for	for	ADP
iajs-2699	24	11	various	various	ADJ
iajs-2699	24	12	applications	application	NOUN
iajs-2699	24	13	in	in	ADP
iajs-2699	24	14	biological	biological	ADJ
iajs-2699	24	15	and	and	CCONJ
iajs-2699	24	16	medical	medical	ADJ
iajs-2699	24	17	research	research	NOUN
iajs-2699	24	18	labs	lab	NOUN
iajs-2699	24	19	.	.	PUNCT
iajs-2699	25	1	these	these	PRON
iajs-2699	25	2	consist	consist	VERB
iajs-2699	25	3	of	of	ADP
iajs-2699	25	4	sequencing	sequence	VERB
iajs-2699	25	5	dna	dna	NOUN
iajs-2699	25	6	cloning	cloning	NOUN
iajs-2699	25	7	,	,	PUNCT
iajs-2699	25	8	gene	gene	NOUN
iajs-2699	25	9	phylogenesis	phylogenesis	NOUN
iajs-2699	25	10	or	or	CCONJ
iajs-2699	25	11	gene	gene	NOUN
iajs-2699	25	12	functional	functional	ADJ
iajs-2699	25	13	analysis	analysis	NOUN
iajs-2699	25	14	,	,	PUNCT
iajs-2699	25	15	identifying	identify	VERB
iajs-2699	25	16	hereditary	hereditary	ADJ
iajs-2699	25	17	diseases	disease	NOUN
iajs-2699	25	18	,	,	PUNCT
iajs-2699	25	19	genetic	genetic	ADJ
iajs-2699	25	20	fingerprints	fingerprint	NOUN
iajs-2699	25	21	(	(	PUNCT
iajs-2699	25	22	useful	useful	ADJ
iajs-2699	25	23	in	in	ADP
iajs-2699	25	24	forensics	forensic	NOUN
iajs-2699	25	25	and	and	CCONJ
iajs-2699	25	26	pathology	pathology	NOUN
iajs-2699	25	27	)	)	PUNCT
iajs-2699	25	28	identification	identification	NOUN
iajs-2699	25	29	,	,	PUNCT
iajs-2699	25	30	and	and	CCONJ
iajs-2699	25	31	infectious	infectious	ADJ
iajs-2699	25	32	disease	disease	NOUN
iajs-2699	25	33	detecting	detect	VERB
iajs-2699	25	34	and	and	CCONJ
iajs-2699	25	35	diagnosis	diagnosis	NOUN
iajs-2699	25	36	.	.	PUNCT
iajs-2699	26	1	in	in	ADP
iajs-2699	26	2	1993	1993	NUM
iajs-2699	26	3	,	,	PUNCT
iajs-2699	26	4	mullis	mullis	PROPN
iajs-2699	26	5	and	and	CCONJ
iajs-2699	26	6	michael	michael	PROPN
iajs-2699	26	7	smith	smith	PROPN
iajs-2699	26	8	,	,	PUNCT
iajs-2699	26	9	for	for	ADP
iajs-2699	26	10	his	his	PRON
iajs-2699	26	11	work	work	NOUN
iajs-2699	26	12	on	on	ADP
iajs-2699	26	13	pcr	pcr	PROPN
iajs-2699	26	14	,	,	PUNCT
iajs-2699	26	15	were	be	AUX
iajs-2699	26	16	awarded	award	VERB
iajs-2699	26	17	a	a	DET
iajs-2699	26	18	nobel	nobel	PROPN
iajs-2699	26	19	prize	prize	NOUN
iajs-2699	26	20	for	for	ADP
iajs-2699	26	21	chemistry[2	chemistry[2	PROPN
iajs-2699	26	22	]	]	PUNCT
iajs-2699	26	23	.	.	PUNCT
iajs-2699	27	1	the	the	DET
iajs-2699	27	2	pcr	pcr	PROPN
iajs-2699	27	3	is	be	AUX
iajs-2699	27	4	usually	usually	ADV
iajs-2699	27	5	performed	perform	VERB
iajs-2699	27	6	in	in	ADP
iajs-2699	27	7	small	small	ADJ
iajs-2699	27	8	reaction	reaction	NOUN
iajs-2699	27	9	tubes	tube	NOUN
iajs-2699	27	10	in	in	ADP
iajs-2699	27	11	the	the	DET
iajs-2699	27	12	thermal	thermal	ADJ
iajs-2699	27	13	cycler	cycler	NOUN
iajs-2699	27	14	in	in	ADP
iajs-2699	27	15	a	a	DET
iajs-2699	27	16	volume	volume	NOUN
iajs-2699	27	17	of	of	ADP
iajs-2699	27	18	10	10	NUM
iajs-2699	27	19	-	-	SYM
iajs-2699	27	20	200	200	NUM
iajs-2699	27	21	ml	ml	NOUN
iajs-2699	27	22	.	.	PUNCT
iajs-2699	28	1	a	a	DET
iajs-2699	28	2	thermal	thermal	ADJ
iajs-2699	28	3	cycler	cycler	NOUN
iajs-2699	28	4	heats	heat	VERB
iajs-2699	28	5	and	and	CCONJ
iajs-2699	28	6	refrigerates	refrigerate	VERB
iajs-2699	28	7	the	the	DET
iajs-2699	28	8	reacting	react	VERB
iajs-2699	28	9	tubes	tube	NOUN
iajs-2699	28	10	for	for	ADP
iajs-2699	28	11	achieving	achieve	VERB
iajs-2699	28	12	the	the	DET
iajs-2699	28	13	necessary	necessary	ADJ
iajs-2699	28	14	temperatures	temperature	NOUN
iajs-2699	28	15	at	at	ADP
iajs-2699	28	16	any	any	DET
iajs-2699	28	17	reaction	reaction	NOUN
iajs-2699	28	18	stage	stage	NOUN
iajs-2699	28	19	.	.	PUNCT
iajs-2699	29	1	numerous	numerous	ADJ
iajs-2699	29	2	modern	modern	ADJ
iajs-2699	29	3	thermal	thermal	ADJ
iajs-2699	29	4	cyclers	cycler	NOUN
iajs-2699	29	5	based	base	VERB
iajs-2699	29	6	on	on	ADP
iajs-2699	29	7	the	the	DET
iajs-2699	29	8	peltier	peltier	ADJ
iajs-2699	29	9	effect	effect	NOUN
iajs-2699	29	10	allow	allow	VERB
iajs-2699	29	11	block	block	NOUN
iajs-2699	29	12	-	-	PUNCT
iajs-2699	29	13	holding	hold	VERB
iajs-2699	29	14	pcr	pcr	ADJ
iajs-2699	29	15	tubes	tube	NOUN
iajs-2699	29	16	to	to	PART
iajs-2699	29	17	be	be	AUX
iajs-2699	29	18	heated	heat	VERB
iajs-2699	29	19	and	and	CCONJ
iajs-2699	29	20	cooled	cool	VERB
iajs-2699	29	21	by	by	ADP
iajs-2699	29	22	simply	simply	ADV
iajs-2699	29	23	retreating	retreat	VERB
iajs-2699	29	24	an	an	DET
iajs-2699	29	25	electric	electric	ADJ
iajs-2699	29	26	current	current	NOUN
iajs-2699	29	27	.	.	PUNCT
iajs-2699	30	1	thin	thin	ADJ
iajs-2699	30	2	-	-	PUNCT
iajs-2699	30	3	walled	wall	VERB
iajs-2699	30	4	reaction	reaction	NOUN
iajs-2699	30	5	tubes	tube	NOUN
iajs-2699	30	6	enable	enable	VERB
iajs-2699	30	7	good	good	ADJ
iajs-2699	30	8	thermal	thermal	ADJ
iajs-2699	30	9	conductivity	conductivity	NOUN
iajs-2699	30	10	for	for	ADP
iajs-2699	30	11	enabling	enable	VERB
iajs-2699	30	12	speedy	speedy	ADJ
iajs-2699	30	13	thermal	thermal	ADJ
iajs-2699	30	14	balance	balance	NOUN
iajs-2699	30	15	.	.	PUNCT
iajs-2699	31	1	the	the	DET
iajs-2699	31	2	majority	majority	NOUN
iajs-2699	31	3	of	of	ADP
iajs-2699	31	4	thermal	thermal	ADJ
iajs-2699	31	5	cyclers	cycler	NOUN
iajs-2699	31	6	possess	possess	VERB
iajs-2699	31	7	heated	heated	ADJ
iajs-2699	31	8	pads	pad	NOUN
iajs-2699	31	9	in	in	ADP
iajs-2699	31	10	the	the	DET
iajs-2699	31	11	reaction	reaction	NOUN
iajs-2699	31	12	tube	tube	NOUN
iajs-2699	31	13	to	to	PART
iajs-2699	31	14	prevent	prevent	VERB
iajs-2699	31	15	condensation	condensation	NOUN
iajs-2699	31	16	.	.	PUNCT
iajs-2699	32	1	older	old	ADJ
iajs-2699	32	2	thermocyclers	thermocycler	NOUN
iajs-2699	32	3	lacking	lack	VERB
iajs-2699	32	4	a	a	DET
iajs-2699	32	5	heated	heated	ADJ
iajs-2699	32	6	lid	lid	NOUN
iajs-2699	32	7	necessitate	necessitate	ADJ
iajs-2699	32	8	an	an	DET
iajs-2699	32	9	oil	oil	NOUN
iajs-2699	32	10	layer	layer	NOUN
iajs-2699	32	11	on	on	ADP
iajs-2699	32	12	the	the	DET
iajs-2699	32	13	upper	upper	ADJ
iajs-2699	32	14	reaction	reaction	NOUN
iajs-2699	32	15	mix	mix	NOUN
iajs-2699	32	16	or	or	CCONJ
iajs-2699	32	17	wax	wax	NOUN
iajs-2699	32	18	ball	ball	NOUN
iajs-2699	32	19	in	in	ADP
iajs-2699	32	20	a	a	DET
iajs-2699	32	21	tube	tube	NOUN
iajs-2699	32	22	.	.	PUNCT
iajs-2699	33	1	2.pcr	2.pcr	ADJ
iajs-2699	33	2	process	process	NOUN
iajs-2699	33	3	multiple	multiple	ADJ
iajs-2699	33	4	basic	basic	ADJ
iajs-2699	33	5	components	component	NOUN
iajs-2699	33	6	are	be	AUX
iajs-2699	33	7	required	require	VERB
iajs-2699	33	8	to	to	ADP
iajs-2699	33	9	pcr	pcr	PROPN
iajs-2699	33	10	based	base	VERB
iajs-2699	33	11	on	on	ADP
iajs-2699	33	12	:	:	PUNCT
iajs-2699	33	13	1dna	1dna	NUM
iajs-2699	33	14	or	or	CCONJ
iajs-2699	33	15	cdna	cdna	PROPN
iajs-2699	33	16	template	template	NOUN
iajs-2699	33	17	containing	contain	VERB
iajs-2699	33	18	the	the	DET
iajs-2699	33	19	dna	dna	PROPN
iajs-2699	33	20	fragment	fragment	NOUN
iajs-2699	33	21	region	region	NOUN
iajs-2699	33	22	to	to	PART
iajs-2699	33	23	be	be	AUX
iajs-2699	33	24	enlarged	enlarge	VERB
iajs-2699	33	25	2dual	2dual	ADJ
iajs-2699	33	26	primers	primer	NOUN
iajs-2699	33	27	that	that	PRON
iajs-2699	33	28	regulate	regulate	VERB
iajs-2699	33	29	the	the	DET
iajs-2699	33	30	start	start	NOUN
iajs-2699	33	31	and	and	CCONJ
iajs-2699	33	32	end	end	NOUN
iajs-2699	33	33	of	of	ADP
iajs-2699	33	34	an	an	DET
iajs-2699	33	35	area	area	NOUN
iajs-2699	33	36	to	to	PART
iajs-2699	33	37	be	be	AUX
iajs-2699	33	38	amplified	amplify	VERB
iajs-2699	33	39	(	(	PUNCT
iajs-2699	33	40	as	as	ADP
iajs-2699	33	41	in	in	ADP
iajs-2699	33	42	the	the	DET
iajs-2699	33	43	next	next	ADJ
iajs-2699	33	44	section	section	NOUN
iajs-2699	33	45	about	about	ADP
iajs-2699	33	46	primers	primer	NOUN
iajs-2699	33	47	)	)	PUNCT
iajs-2699	33	48	3taq	3taq	NUM
iajs-2699	33	49	polymerase	polymerase	NOUN
iajs-2699	33	50	that	that	PRON
iajs-2699	33	51	copies	copy	VERB
iajs-2699	33	52	the	the	DET
iajs-2699	33	53	amplified	amplified	ADJ
iajs-2699	33	54	region	region	NOUN
iajs-2699	33	55	4nucleotides	4nucleotide	NOUN
iajs-2699	33	56	,	,	PUNCT
iajs-2699	33	57	the	the	DET
iajs-2699	33	58	dna	dna	NOUN
iajs-2699	33	59	-	-	PUNCT
iajs-2699	33	60	polymerase	polymerase	NOUN
iajs-2699	33	61	for	for	ADP
iajs-2699	33	62	new	new	ADJ
iajs-2699	33	63	dna	dna	PROPN
iajs-2699	33	64	5buffer	5buffer	NUM
iajs-2699	33	65	that	that	PRON
iajs-2699	33	66	offers	offer	VERB
iajs-2699	33	67	an	an	DET
iajs-2699	33	68	appropriate	appropriate	ADJ
iajs-2699	33	69	dna	dna	NOUN
iajs-2699	33	70	polymerase	polymerase	NOUN
iajs-2699	33	71	chemical	chemical	NOUN
iajs-2699	33	72	surroundings	surrounding	NOUN
iajs-2699	33	73	.	.	PUNCT
iajs-2699	34	1	a	a	DET
iajs-2699	34	2	thermal	thermal	ADJ
iajs-2699	34	3	cycler	cycler	NOUN
iajs-2699	34	4	performs	perform	VERB
iajs-2699	34	5	the	the	DET
iajs-2699	34	6	pcr	pcr	ADJ
iajs-2699	34	7	reaction	reaction	NOUN
iajs-2699	34	8	.	.	PUNCT
iajs-2699	35	1	this	this	DET
iajs-2699	35	2	machine	machine	NOUN
iajs-2699	35	3	heats	heat	VERB
iajs-2699	35	4	and	and	CCONJ
iajs-2699	35	5	cools	cool	VERB
iajs-2699	35	6	the	the	DET
iajs-2699	35	7	reacting	react	VERB
iajs-2699	35	8	tubes	tube	NOUN
iajs-2699	35	9	at	at	ADP
iajs-2699	35	10	a	a	DET
iajs-2699	35	11	required	require	VERB
iajs-2699	35	12	temperature	temperature	NOUN
iajs-2699	35	13	for	for	ADP
iajs-2699	35	14	every	every	DET
iajs-2699	35	15	reaction	reaction	NOUN
iajs-2699	35	16	step	step	NOUN
iajs-2699	35	17	.	.	PUNCT
iajs-2699	36	1	a	a	DET
iajs-2699	36	2	heated	heated	ADJ
iajs-2699	36	3	deck	deck	NOUN
iajs-2699	36	4	is	be	AUX
iajs-2699	36	5	positioned	position	VERB
iajs-2699	36	6	on	on	ADP
iajs-2699	36	7	the	the	DET
iajs-2699	36	8	upper	upper	ADJ
iajs-2699	36	9	reaction	reaction	NOUN
iajs-2699	36	10	tube	tube	NOUN
iajs-2699	36	11	to	to	PART
iajs-2699	36	12	avoid	avoid	VERB
iajs-2699	36	13	evaporating	evaporate	VERB
iajs-2699	36	14	the	the	DET
iajs-2699	36	15	reaction	reaction	NOUN
iajs-2699	36	16	mixture	mixture	NOUN
iajs-2699	36	17	(	(	PUNCT
iajs-2699	36	18	normally	normally	ADV
iajs-2699	36	19	volumes	volume	NOUN
iajs-2699	36	20	range	range	VERB
iajs-2699	36	21	from	from	ADP
iajs-2699	36	22	15	15	NUM
iajs-2699	36	23	to	to	PART
iajs-2699	36	24	100	100	NUM
iajs-2699	36	25	l	l	NOUN
iajs-2699	36	26	per	per	ADP
iajs-2699	36	27	tube	tube	NOUN
iajs-2699	36	28	)	)	PUNCT
iajs-2699	36	29	,	,	PUNCT
iajs-2699	36	30	or	or	CCONJ
iajs-2699	36	31	an	an	DET
iajs-2699	36	32	oil	oil	NOUN
iajs-2699	36	33	layer	layer	NOUN
iajs-2699	36	34	can	can	AUX
iajs-2699	36	35	be	be	AUX
iajs-2699	36	36	placed	place	VERB
iajs-2699	36	37	on	on	ADP
iajs-2699	36	38	a	a	DET
iajs-2699	36	39	reaction	reaction	NOUN
iajs-2699	36	40	mixture	mixture	NOUN
iajs-2699	36	41	surface	surface	NOUN
iajs-2699	36	42	.	.	PUNCT
iajs-2699	37	1	pcr	pcr	PROPN
iajs-2699	37	2	is	be	AUX
iajs-2699	37	3	an	an	DET
iajs-2699	37	4	enzymatic	enzymatic	ADJ
iajs-2699	37	5	test	test	NOUN
iajs-2699	37	6	that	that	PRON
iajs-2699	37	7	can	can	AUX
iajs-2699	37	8	enhance	enhance	VERB
iajs-2699	37	9	a	a	DET
iajs-2699	37	10	particular	particular	ADJ
iajs-2699	37	11	dna	dna	NOUN
iajs-2699	37	12	fragment	fragment	NOUN
iajs-2699	37	13	from	from	ADP
iajs-2699	37	14	a	a	DET
iajs-2699	37	15	complex	complex	ADJ
iajs-2699	37	16	dna	dna	NOUN
iajs-2699	37	17	body	body	NOUN
iajs-2699	37	18	,	,	PUNCT
iajs-2699	37	19	a	a	DET
iajs-2699	37	20	simple	simple	ADJ
iajs-2699	37	21	yet	yet	ADV
iajs-2699	37	22	elegant	elegant	ADJ
iajs-2699	37	23	one	one	NUM
iajs-2699	37	24	.	.	PUNCT
iajs-2699	38	1	dr	dr	PROPN
iajs-2699	38	2	.	.	PROPN
iajs-2699	38	3	kary	kary	PROPN
iajs-2699	38	4	mullis	mullis	PROPN
iajs-2699	38	5	found	find	VERB
iajs-2699	38	6	that	that	SCONJ
iajs-2699	38	7	the	the	DET
iajs-2699	38	8	pcr	pcr	PROPN
iajs-2699	38	9	test	test	NOUN
iajs-2699	38	10	"	"	PUNCT
iajs-2699	38	11	may	may	AUX
iajs-2699	38	12	let	let	VERB
iajs-2699	38	13	you	you	PRON
iajs-2699	38	14	determine	determine	VERB
iajs-2699	38	15	the	the	DET
iajs-2699	38	16	piece	piece	NOUN
iajs-2699	38	17	of	of	ADP
iajs-2699	38	18	dna	dna	PROPN
iajs-2699	38	19	that	that	PRON
iajs-2699	38	20	you	you	PRON
iajs-2699	38	21	want	want	VERB
iajs-2699	38	22	and	and	CCONJ
iajs-2699	38	23	have	have	AUX
iajs-2699	38	24	based	base	VERB
iajs-2699	38	25	on	on	ADP
iajs-2699	38	26	your	your	PRON
iajs-2699	38	27	requirement	requirement	NOUN
iajs-2699	38	28	"	"	PUNCT
iajs-2699	38	29	three	three	NUM
iajs-2699	38	30	pcr	pcr	NOUN
iajs-2699	38	31	can	can	AUX
iajs-2699	38	32	be	be	AUX
iajs-2699	38	33	done	do	VERB
iajs-2699	38	34	using	use	VERB
iajs-2699	38	35	various	various	ADJ
iajs-2699	38	36	tissues	tissue	NOUN
iajs-2699	38	37	,	,	PUNCT
iajs-2699	38	38	including	include	VERB
iajs-2699	38	39	peripheral	peripheral	ADJ
iajs-2699	38	40	blood	blood	NOUN
iajs-2699	38	41	,	,	PUNCT
iajs-2699	38	42	saliva	saliva	NOUN
iajs-2699	38	43	,	,	PUNCT
iajs-2699	38	44	hair	hair	NOUN
iajs-2699	38	45	,	,	PUNCT
iajs-2699	38	46	skin	skin	NOUN
iajs-2699	38	47	,	,	PUNCT
iajs-2699	38	48	and	and	CCONJ
iajs-2699	38	49	microbes	microbe	NOUN
iajs-2699	38	50	,	,	PUNCT
iajs-2699	38	51	as	as	ADP
iajs-2699	38	52	the	the	DET
iajs-2699	38	53	dna	dna	PROPN
iajs-2699	38	54	source	source	NOUN
iajs-2699	38	55	.	.	PUNCT
iajs-2699	39	1	for	for	ADP
iajs-2699	39	2	generating	generate	VERB
iajs-2699	39	3	sufficient	sufficient	ADJ
iajs-2699	39	4	copies	copy	NOUN
iajs-2699	39	5	for	for	ADP
iajs-2699	39	6	analysis	analysis	NOUN
iajs-2699	39	7	by	by	ADP
iajs-2699	39	8	means	mean	NOUN
iajs-2699	39	9	of	of	ADP
iajs-2699	39	10	ibn	ibn	PROPN
iajs-2699	39	11	al	al	PROPN
iajs-2699	39	12	-	-	PUNCT
iajs-2699	39	13	haitham	haitham	PROPN
iajs-2699	39	14	jour	jour	X
iajs-2699	39	15	.	.	PROPN
iajs-2699	40	1	for	for	ADP
iajs-2699	40	2	pure	pure	ADJ
iajs-2699	40	3	&	&	CCONJ
iajs-2699	40	4	appl	appl	PROPN
iajs-2699	40	5	.	.	PUNCT
iajs-2699	41	1	sci	sci	PROPN
iajs-2699	41	2	.	.	PROPN
iajs-2699	42	1	34(4)2021	34(4)2021	NUM
iajs-2699	42	2	37	37	NUM
iajs-2699	42	3	conventional	conventional	ADJ
iajs-2699	42	4	lab	lab	NOUN
iajs-2699	42	5	methods	method	NOUN
iajs-2699	42	6	for	for	ADP
iajs-2699	42	7	pcr	pcr	NOUN
iajs-2699	42	8	,	,	PUNCT
iajs-2699	42	9	just	just	ADV
iajs-2699	42	10	trace	trace	NOUN
iajs-2699	42	11	quantities	quantity	NOUN
iajs-2699	42	12	of	of	ADP
iajs-2699	42	13	dna	dna	PROPN
iajs-2699	42	14	are	be	AUX
iajs-2699	42	15	required	require	VERB
iajs-2699	42	16	.	.	PUNCT
iajs-2699	43	1	pcr	pcr	PROPN
iajs-2699	43	2	is	be	AUX
iajs-2699	43	3	a	a	DET
iajs-2699	43	4	sensitive	sensitive	ADJ
iajs-2699	43	5	test	test	NOUN
iajs-2699	43	6	,	,	PUNCT
iajs-2699	43	7	therefore	therefore	ADV
iajs-2699	43	8	.	.	PUNCT
iajs-2699	44	1	the	the	DET
iajs-2699	44	2	dna	dna	PROPN
iajs-2699	44	3	,	,	PUNCT
iajs-2699	44	4	primers	primer	NOUN
iajs-2699	44	5	,	,	PUNCT
iajs-2699	44	6	nucleotides	nucleotide	NOUN
iajs-2699	44	7	,	,	PUNCT
iajs-2699	44	8	and	and	CCONJ
iajs-2699	44	9	dna	dna	PROPN
iajs-2699	44	10	polymerases	polymerase	NOUN
iajs-2699	44	11	template	template	NOUN
iajs-2699	44	12	must	must	AUX
iajs-2699	44	13	be	be	AUX
iajs-2699	44	14	available	available	ADJ
iajs-2699	44	15	for	for	SCONJ
iajs-2699	44	16	every	every	DET
iajs-2699	44	17	pcr	pcr	NOUN
iajs-2699	44	18	assay	assay	VERB
iajs-2699	44	19	.	.	PUNCT
iajs-2699	45	1	the	the	DET
iajs-2699	45	2	main	main	ADJ
iajs-2699	45	3	enzyme	enzyme	NOUN
iajs-2699	45	4	,	,	PUNCT
iajs-2699	45	5	which	which	PRON
iajs-2699	45	6	associates	associate	VERB
iajs-2699	45	7	distinct	distinct	ADJ
iajs-2699	45	8	nucleotides	nucleotide	NOUN
iajs-2699	45	9	for	for	ADP
iajs-2699	45	10	forming	form	VERB
iajs-2699	45	11	the	the	DET
iajs-2699	45	12	pcr	pcr	ADJ
iajs-2699	45	13	product	product	NOUN
iajs-2699	45	14	,	,	PUNCT
iajs-2699	45	15	stands	stand	VERB
iajs-2699	45	16	for	for	ADP
iajs-2699	45	17	dna	dna	PROPN
iajs-2699	45	18	polymerase	polymerase	NOUN
iajs-2699	45	19	.	.	PUNCT
iajs-2699	46	1	nucleotides	nucleotide	NOUN
iajs-2699	46	2	have	have	VERB
iajs-2699	46	3	four	four	NUM
iajs-2699	46	4	basic	basic	ADJ
iajs-2699	46	5	components	component	NOUN
iajs-2699	46	6	(	(	PUNCT
iajs-2699	46	7	a	a	DET
iajs-2699	46	8	,	,	PUNCT
iajs-2699	46	9	t	t	PROPN
iajs-2699	46	10	,	,	PUNCT
iajs-2699	46	11	c	c	X
iajs-2699	46	12	,	,	PUNCT
iajs-2699	46	13	g	g	NOUN
iajs-2699	46	14	)	)	PUNCT
iajs-2699	46	15	in	in	ADP
iajs-2699	46	16	dna	dna	PROPN
iajs-2699	46	17	:	:	PUNCT
iajs-2699	46	18	adenine	adenine	NOUN
iajs-2699	46	19	,	,	PUNCT
iajs-2699	46	20	thymine	thymine	NOUN
iajs-2699	46	21	,	,	PUNCT
iajs-2699	46	22	cytosine	cytosine	NOUN
iajs-2699	46	23	,	,	PUNCT
iajs-2699	46	24	and	and	CCONJ
iajs-2699	46	25	guanine	guanine	NOUN
iajs-2699	46	26	.	.	PUNCT
iajs-2699	47	1	these	these	PRON
iajs-2699	47	2	are	be	AUX
iajs-2699	47	3	the	the	DET
iajs-2699	47	4	building	building	NOUN
iajs-2699	47	5	blocks	block	NOUN
iajs-2699	47	6	used	use	VERB
iajs-2699	47	7	to	to	PART
iajs-2699	47	8	create	create	VERB
iajs-2699	47	9	of	of	ADP
iajs-2699	47	10	the	the	DET
iajs-2699	47	11	resultant	resultant	NOUN
iajs-2699	47	12	product	product	NOUN
iajs-2699	47	13	of	of	ADP
iajs-2699	47	14	pcr	pcr	NOUN
iajs-2699	47	15	by	by	ADP
iajs-2699	47	16	the	the	DET
iajs-2699	47	17	dna	dna	PROPN
iajs-2699	47	18	polymerase	polymerase	NOUN
iajs-2699	47	19	.	.	PUNCT
iajs-2699	48	1	the	the	DET
iajs-2699	48	2	primers	primer	NOUN
iajs-2699	48	3	identify	identify	VERB
iajs-2699	48	4	a	a	DET
iajs-2699	48	5	particular	particular	ADJ
iajs-2699	48	6	dna	dna	NOUN
iajs-2699	48	7	product	product	NOUN
iajs-2699	48	8	to	to	PART
iajs-2699	48	9	be	be	AUX
iajs-2699	48	10	amplified	amplify	VERB
iajs-2699	48	11	in	in	ADP
iajs-2699	48	12	a	a	DET
iajs-2699	48	13	reaction	reaction	NOUN
iajs-2699	48	14	.	.	PUNCT
iajs-2699	49	1	the	the	DET
iajs-2699	49	2	primers	primer	NOUN
iajs-2699	49	3	stand	stand	VERB
iajs-2699	49	4	for	for	ADP
iajs-2699	49	5	small	small	ADJ
iajs-2699	49	6	dna	dna	NOUN
iajs-2699	49	7	fragments	fragment	NOUN
iajs-2699	49	8	with	with	ADP
iajs-2699	49	9	an	an	DET
iajs-2699	49	10	arrangement	arrangement	NOUN
iajs-2699	49	11	to	to	PART
iajs-2699	49	12	complement	complement	VERB
iajs-2699	49	13	and	and	CCONJ
iajs-2699	49	14	amplify	amplify	VERB
iajs-2699	49	15	the	the	DET
iajs-2699	49	16	target	target	NOUN
iajs-2699	49	17	dna	dna	NOUN
iajs-2699	49	18	.	.	PUNCT
iajs-2699	50	1	the	the	DET
iajs-2699	50	2	dna	dna	PROPN
iajs-2699	50	3	polymerase	polymerase	NOUN
iajs-2699	50	4	is	be	AUX
iajs-2699	50	5	supplemented	supplement	VERB
iajs-2699	50	6	by	by	ADP
iajs-2699	50	7	these	these	PRON
iajs-2699	50	8	as	as	ADP
iajs-2699	50	9	an	an	DET
iajs-2699	50	10	expanding	expand	VERB
iajs-2699	50	11	point	point	NOUN
iajs-2699	50	12	.	.	PUNCT
iajs-2699	51	1	the	the	DET
iajs-2699	51	2	components	component	NOUN
iajs-2699	51	3	have	have	AUX
iajs-2699	51	4	been	be	AUX
iajs-2699	51	5	mixed	mix	VERB
iajs-2699	51	6	in	in	ADP
iajs-2699	51	7	a	a	DET
iajs-2699	51	8	96	96	NUM
iajs-2699	51	9	-	-	PUNCT
iajs-2699	51	10	well	well	NOUN
iajs-2699	51	11	tube	tube	NOUN
iajs-2699	51	12	and	and	CCONJ
iajs-2699	51	13	then	then	ADV
iajs-2699	51	14	positioned	position	VERB
iajs-2699	51	15	in	in	ADP
iajs-2699	51	16	the	the	DET
iajs-2699	51	17	machine	machine	NOUN
iajs-2699	51	18	that	that	PRON
iajs-2699	51	19	enables	enable	VERB
iajs-2699	51	20	repetitive	repetitive	ADJ
iajs-2699	51	21	dna	dna	NOUN
iajs-2699	51	22	amplifying	amplify	VERB
iajs-2699	51	23	cycles	cycle	NOUN
iajs-2699	51	24	to	to	PART
iajs-2699	51	25	be	be	AUX
iajs-2699	51	26	carried	carry	VERB
iajs-2699	51	27	out	out	ADP
iajs-2699	51	28	in	in	ADP
iajs-2699	51	29	3	3	NUM
iajs-2699	51	30	elementary	elementary	ADJ
iajs-2699	51	31	steps	step	NOUN
iajs-2699	51	32	.	.	PUNCT
iajs-2699	52	1	most	most	ADJ
iajs-2699	52	2	of	of	ADP
iajs-2699	52	3	the	the	DET
iajs-2699	52	4	machine	machine	NOUN
iajs-2699	52	5	stands	stand	VERB
iajs-2699	52	6	for	for	ADP
iajs-2699	52	7	a	a	DET
iajs-2699	52	8	thermal	thermal	ADJ
iajs-2699	52	9	cycler	cycler	NOUN
iajs-2699	52	10	.	.	PUNCT
iajs-2699	53	1	it	it	PRON
iajs-2699	53	2	has	have	VERB
iajs-2699	53	3	a	a	DET
iajs-2699	53	4	hollow	hollow	ADJ
iajs-2699	53	5	thermal	thermal	ADJ
iajs-2699	53	6	block	block	NOUN
iajs-2699	53	7	.	.	PUNCT
iajs-2699	54	1	pcr	pcr	ADJ
iajs-2699	54	2	reaction	reaction	NOUN
iajs-2699	54	3	mixture	mixture	NOUN
iajs-2699	54	4	testing	testing	NOUN
iajs-2699	54	5	tubes	tube	NOUN
iajs-2699	54	6	or	or	CCONJ
iajs-2699	54	7	plates	plate	NOUN
iajs-2699	54	8	have	have	AUX
iajs-2699	54	9	been	be	AUX
iajs-2699	54	10	added	add	VERB
iajs-2699	54	11	.	.	PUNCT
iajs-2699	55	1	indistinct	indistinct	ADJ
iajs-2699	55	2	,	,	PUNCT
iajs-2699	55	3	accurate	accurate	ADJ
iajs-2699	55	4	,	,	PUNCT
iajs-2699	55	5	and	and	CCONJ
iajs-2699	55	6	pre	pre	VERB
iajs-2699	55	7	-	-	ADJ
iajs-2699	55	8	programmed	programmed	ADJ
iajs-2699	55	9	steps	step	NOUN
iajs-2699	55	10	the	the	DET
iajs-2699	55	11	machine	machine	NOUN
iajs-2699	55	12	increases	increase	VERB
iajs-2699	55	13	and	and	CCONJ
iajs-2699	55	14	lowers	lower	VERB
iajs-2699	55	15	block	block	NOUN
iajs-2699	55	16	temperatures	temperature	NOUN
iajs-2699	55	17	[	[	X
iajs-2699	55	18	4	4	NUM
iajs-2699	55	19	]	]	PUNCT
iajs-2699	55	20	.	.	PUNCT
iajs-2699	56	1	first	first	ADV
iajs-2699	56	2	of	of	ADP
iajs-2699	56	3	all	all	PRON
iajs-2699	56	4	,	,	PUNCT
iajs-2699	56	5	it	it	PRON
iajs-2699	56	6	is	be	AUX
iajs-2699	56	7	heated	heat	VERB
iajs-2699	56	8	higher	high	ADJ
iajs-2699	56	9	than	than	ADP
iajs-2699	56	10	a	a	DET
iajs-2699	56	11	melting	melting	NOUN
iajs-2699	56	12	point	point	NOUN
iajs-2699	56	13	for	for	ADP
iajs-2699	56	14	dual	dual	ADJ
iajs-2699	56	15	complementary	complementary	ADJ
iajs-2699	56	16	dna	dna	PROPN
iajs-2699	56	17	strands	strand	NOUN
iajs-2699	56	18	of	of	ADP
iajs-2699	56	19	a	a	DET
iajs-2699	56	20	target	target	NOUN
iajs-2699	56	21	dna	dna	NOUN
iajs-2699	56	22	,	,	PUNCT
iajs-2699	56	23	enabling	enable	VERB
iajs-2699	56	24	a	a	DET
iajs-2699	56	25	denaturation	denaturation	NOUN
iajs-2699	56	26	process	process	NOUN
iajs-2699	56	27	to	to	PART
iajs-2699	56	28	be	be	AUX
iajs-2699	56	29	separated	separate	VERB
iajs-2699	56	30	among	among	ADP
iajs-2699	56	31	the	the	DET
iajs-2699	56	32	strands	strand	NOUN
iajs-2699	56	33	.	.	PUNCT
iajs-2699	57	1	then	then	ADV
iajs-2699	57	2	the	the	DET
iajs-2699	57	3	temperature	temperature	NOUN
iajs-2699	57	4	is	be	AUX
iajs-2699	57	5	reduced	reduce	VERB
iajs-2699	57	6	to	to	PART
iajs-2699	57	7	connect	connect	VERB
iajs-2699	57	8	the	the	DET
iajs-2699	57	9	specific	specific	ADJ
iajs-2699	57	10	primers	primer	NOUN
iajs-2699	57	11	with	with	ADP
iajs-2699	57	12	the	the	DET
iajs-2699	57	13	target	target	NOUN
iajs-2699	57	14	dna	dna	NOUN
iajs-2699	57	15	segments	segment	NOUN
iajs-2699	57	16	,	,	PUNCT
iajs-2699	57	17	called	call	VERB
iajs-2699	57	18	a	a	DET
iajs-2699	57	19	hybridization	hybridization	NOUN
iajs-2699	57	20	process	process	NOUN
iajs-2699	57	21	.	.	PUNCT
iajs-2699	58	1	hybridization	hybridization	NOUN
iajs-2699	58	2	among	among	ADP
iajs-2699	58	3	target	target	NOUN
iajs-2699	58	4	dna	dna	NOUN
iajs-2699	58	5	and	and	CCONJ
iajs-2699	58	6	primers	primer	NOUN
iajs-2699	58	7	occurs	occur	VERB
iajs-2699	58	8	as	as	SCONJ
iajs-2699	58	9	they	they	PRON
iajs-2699	58	10	have	have	AUX
iajs-2699	58	11	been	be	AUX
iajs-2699	58	12	sequence	sequence	NOUN
iajs-2699	58	13	-	-	PUNCT
iajs-2699	58	14	complementary	complementary	ADJ
iajs-2699	58	15	(	(	PUNCT
iajs-2699	58	16	as	as	ADP
iajs-2699	58	17	in	in	ADP
iajs-2699	58	18	a	a	DET
iajs-2699	58	19	binding	binding	NOUN
iajs-2699	58	20	to	to	ADP
iajs-2699	58	21	g	g	NOUN
iajs-2699	58	22	)	)	PUNCT
iajs-2699	58	23	.	.	PUNCT
iajs-2699	59	1	temperature	temperature	NOUN
iajs-2699	59	2	can	can	AUX
iajs-2699	59	3	be	be	AUX
iajs-2699	59	4	elevated	elevate	VERB
iajs-2699	59	5	again	again	ADV
iajs-2699	59	6	,	,	PUNCT
iajs-2699	59	7	as	as	SCONJ
iajs-2699	59	8	the	the	DET
iajs-2699	59	9	dna	dna	PROPN
iajs-2699	59	10	polymerase	polymerase	NOUN
iajs-2699	59	11	has	have	AUX
iajs-2699	59	12	been	be	AUX
iajs-2699	59	13	capable	capable	ADJ
iajs-2699	59	14	of	of	ADP
iajs-2699	59	15	extending	extend	VERB
iajs-2699	59	16	the	the	DET
iajs-2699	59	17	primers	primer	NOUN
iajs-2699	59	18	through	through	ADP
iajs-2699	59	19	inserting	insert	VERB
iajs-2699	59	20	nucleotides	nucleotide	NOUN
iajs-2699	59	21	to	to	ADP
iajs-2699	59	22	a	a	DET
iajs-2699	59	23	developing	develop	VERB
iajs-2699	59	24	dna	dna	NOUN
iajs-2699	59	25	strand	strand	NOUN
iajs-2699	59	26	,	,	PUNCT
iajs-2699	59	27	as	as	SCONJ
iajs-2699	59	28	depicted	depict	VERB
iajs-2699	59	29	in	in	ADP
iajs-2699	59	30	figure	figure	NOUN
iajs-2699	59	31	1	1	NUM
iajs-2699	59	32	.	.	PUNCT
iajs-2699	60	1	an	an	DET
iajs-2699	60	2	amount	amount	NOUN
iajs-2699	60	3	of	of	ADP
iajs-2699	60	4	copied	copy	VERB
iajs-2699	60	5	dna	dna	NOUN
iajs-2699	60	6	molecules	molecule	NOUN
iajs-2699	60	7	doubles	double	VERB
iajs-2699	60	8	each	each	DET
iajs-2699	60	9	time	time	NOUN
iajs-2699	60	10	based	base	VERB
iajs-2699	60	11	on	on	ADP
iajs-2699	60	12	these	these	DET
iajs-2699	60	13	three	three	NUM
iajs-2699	60	14	repeated	repeat	VERB
iajs-2699	60	15	steps	step	NOUN
iajs-2699	60	16	.	.	PUNCT
iajs-2699	61	1	3.primers	3.primers	NUM
iajs-2699	61	2	the	the	DET
iajs-2699	61	3	amplified	amplify	VERB
iajs-2699	61	4	dna	dna	PROPN
iajs-2699	61	5	fragment	fragment	NOUN
iajs-2699	61	6	is	be	AUX
iajs-2699	61	7	determined	determine	VERB
iajs-2699	61	8	based	base	VERB
iajs-2699	61	9	on	on	ADP
iajs-2699	61	10	selecting	select	VERB
iajs-2699	61	11	primers	primer	NOUN
iajs-2699	61	12	in	in	ADP
iajs-2699	61	13	addition	addition	NOUN
iajs-2699	61	14	to	to	ADP
iajs-2699	61	15	the	the	DET
iajs-2699	61	16	start	start	NOUN
iajs-2699	61	17	and	and	CCONJ
iajs-2699	61	18	end	end	NOUN
iajs-2699	61	19	of	of	ADP
iajs-2699	61	20	the	the	DET
iajs-2699	61	21	dna	dna	PROPN
iajs-2699	61	22	fragment	fragment	NOUN
iajs-2699	61	23	.	.	PUNCT
iajs-2699	62	1	the	the	DET
iajs-2699	62	2	primers	primer	NOUN
iajs-2699	62	3	stand	stand	VERB
iajs-2699	62	4	for	for	ADP
iajs-2699	62	5	short	short	ADJ
iajs-2699	62	6	,	,	PUNCT
iajs-2699	62	7	artificial	artificial	ADJ
iajs-2699	62	8	dna	dna	NOUN
iajs-2699	62	9	stripes	stripe	NOUN
iajs-2699	62	10	,	,	PUNCT
iajs-2699	62	11	no	no	ADV
iajs-2699	62	12	higher	high	ADJ
iajs-2699	62	13	than	than	ADP
iajs-2699	62	14	fifty	fifty	NUM
iajs-2699	62	15	(	(	PUNCT
iajs-2699	62	16	typically	typically	ADV
iajs-2699	62	17	18	18	NUM
iajs-2699	62	18	-	-	PUNCT
iajs-2699	62	19	25bp	25bp	NOUN
iajs-2699	62	20	)	)	PUNCT
iajs-2699	62	21	nucleotides	nucleotide	NOUN
iajs-2699	62	22	have	have	AUX
iajs-2699	62	23	been	be	AUX
iajs-2699	62	24	based	base	VERB
iajs-2699	62	25	on	on	ADP
iajs-2699	62	26	a	a	DET
iajs-2699	62	27	starting	starting	NOUN
iajs-2699	62	28	and	and	CCONJ
iajs-2699	62	29	ending	ending	NOUN
iajs-2699	62	30	of	of	ADP
iajs-2699	62	31	dna	dna	PROPN
iajs-2699	62	32	fragment	fragment	NOUN
iajs-2699	62	33	to	to	PART
iajs-2699	62	34	be	be	AUX
iajs-2699	62	35	amplified	amplify	VERB
iajs-2699	62	36	.	.	PUNCT
iajs-2699	63	1	dna	dna	NOUN
iajs-2699	63	2	-	-	PUNCT
iajs-2699	63	3	polymerase	polymerase	NOUN
iajs-2699	63	4	connects	connect	VERB
iajs-2699	63	5	and	and	CCONJ
iajs-2699	63	6	starts	start	VERB
iajs-2699	63	7	a	a	DET
iajs-2699	63	8	new	new	ADJ
iajs-2699	63	9	dna	dna	NOUN
iajs-2699	63	10	strand	strand	NOUN
iajs-2699	63	11	synthesis	synthesis	NOUN
iajs-2699	63	12	,	,	PUNCT
iajs-2699	63	13	they	they	PRON
iajs-2699	63	14	are	be	AUX
iajs-2699	63	15	annealed	anneal	VERB
iajs-2699	63	16	(	(	PUNCT
iajs-2699	63	17	adhere	adhere	NOUN
iajs-2699	63	18	)	)	PUNCT
iajs-2699	63	19	to	to	ADP
iajs-2699	63	20	a	a	DET
iajs-2699	63	21	dna	dna	PROPN
iajs-2699	63	22	template	template	NOUN
iajs-2699	63	23	.	.	PUNCT
iajs-2699	64	1	ibn	ibn	PROPN
iajs-2699	64	2	al	al	PROPN
iajs-2699	64	3	-	-	PUNCT
iajs-2699	64	4	haitham	haitham	PROPN
iajs-2699	64	5	jour	jour	X
iajs-2699	64	6	.	.	PROPN
iajs-2699	64	7	for	for	ADP
iajs-2699	64	8	pure	pure	ADJ
iajs-2699	64	9	&	&	CCONJ
iajs-2699	64	10	appl	appl	PROPN
iajs-2699	64	11	.	.	PUNCT
iajs-2699	65	1	sci	sci	PROPN
iajs-2699	65	2	.	.	PROPN
iajs-2699	66	1	34(4)2021	34(4)2021	NUM
iajs-2699	66	2	38	38	NUM
iajs-2699	66	3	figure	figure	NOUN
iajs-2699	66	4	1	1	NUM
iajs-2699	66	5	:	:	PUNCT
iajs-2699	66	6	pcr	pcr	ADJ
iajs-2699	66	7	process	process	NOUN
iajs-2699	66	8	and	and	CCONJ
iajs-2699	66	9	thermal	thermal	ADJ
iajs-2699	66	10	cycle	cycle	NOUN
iajs-2699	66	11	(	(	PUNCT
iajs-2699	66	12	weier	weier	X
iajs-2699	66	13	et	et	PROPN
iajs-2699	66	14	al	al	PROPN
iajs-2699	66	15	,	,	PUNCT
iajs-2699	66	16	1988	1988	NUM
iajs-2699	66	17	)	)	PUNCT
iajs-2699	66	18	4.analyzing	4.analyzing	NUM
iajs-2699	66	19	pcr	pcr	ADJ
iajs-2699	66	20	product	product	NOUN
iajs-2699	66	21	the	the	DET
iajs-2699	66	22	pcr	pcr	ADJ
iajs-2699	66	23	products	product	NOUN
iajs-2699	66	24	can	can	AUX
iajs-2699	66	25	be	be	AUX
iajs-2699	66	26	visualized	visualize	VERB
iajs-2699	66	27	by	by	ADP
iajs-2699	66	28	dual	dual	ADJ
iajs-2699	66	29	foremost	foremost	ADJ
iajs-2699	66	30	methods	method	NOUN
iajs-2699	66	31	:	:	PUNCT
iajs-2699	66	32	(	(	PUNCT
iajs-2699	66	33	1	1	X
iajs-2699	66	34	)	)	PUNCT
iajs-2699	66	35	staining	stain	VERB
iajs-2699	66	36	of	of	ADP
iajs-2699	66	37	the	the	DET
iajs-2699	66	38	product	product	NOUN
iajs-2699	66	39	of	of	ADP
iajs-2699	66	40	dna	dna	PROPN
iajs-2699	66	41	amplified	amplify	VERB
iajs-2699	66	42	by	by	ADP
iajs-2699	66	43	a	a	DET
iajs-2699	66	44	chemical	chemical	ADJ
iajs-2699	66	45	dye	dye	NOUN
iajs-2699	66	46	like	like	ADP
iajs-2699	66	47	bromide	bromide	NOUN
iajs-2699	66	48	ethidium	ethidium	NOUN
iajs-2699	66	49	,	,	PUNCT
iajs-2699	66	50	that	that	PRON
iajs-2699	66	51	is	be	AUX
iajs-2699	66	52	intercalated	intercalate	VERB
iajs-2699	66	53	among	among	ADP
iajs-2699	66	54	dual	dual	ADJ
iajs-2699	66	55	duplex	duplex	NOUN
iajs-2699	66	56	strands	strand	NOUN
iajs-2699	66	57	or	or	CCONJ
iajs-2699	66	58	(	(	PUNCT
iajs-2699	66	59	2	2	X
iajs-2699	66	60	)	)	PUNCT
iajs-2699	66	61	marking	marking	NOUN
iajs-2699	66	62	of	of	ADP
iajs-2699	66	63	fluorescent	fluorescent	ADJ
iajs-2699	66	64	dyes	dye	NOUN
iajs-2699	66	65	(	(	PUNCT
iajs-2699	66	66	fluorophores	fluorophore	NOUN
iajs-2699	66	67	)	)	PUNCT
iajs-2699	66	68	pcr	pcr	ADJ
iajs-2699	66	69	primers	primer	NOUN
iajs-2699	66	70	or	or	CCONJ
iajs-2699	66	71	nucleotides	nucleotide	NOUN
iajs-2699	66	72	before	before	ADP
iajs-2699	66	73	amplification	amplification	NOUN
iajs-2699	66	74	of	of	ADP
iajs-2699	66	75	pcrs	pcrs	PROPN
iajs-2699	66	76	.	.	PUNCT
iajs-2699	67	1	this	this	DET
iajs-2699	67	2	method	method	NOUN
iajs-2699	67	3	permits	permit	VERB
iajs-2699	67	4	the	the	DET
iajs-2699	67	5	incorporation	incorporation	NOUN
iajs-2699	67	6	of	of	ADP
iajs-2699	67	7	the	the	DET
iajs-2699	67	8	labels	label	NOUN
iajs-2699	67	9	directly	directly	ADV
iajs-2699	67	10	into	into	ADP
iajs-2699	67	11	the	the	DET
iajs-2699	67	12	pcr	pcr	ADJ
iajs-2699	67	13	product	product	NOUN
iajs-2699	67	14	.	.	PUNCT
iajs-2699	68	1	an	an	DET
iajs-2699	68	2	agarose	agarose	NOUN
iajs-2699	68	3	gel	gel	NOUN
iajs-2699	68	4	electrophoresis	electrophoresis	NOUN
iajs-2699	68	5	,	,	PUNCT
iajs-2699	68	6	which	which	PRON
iajs-2699	68	7	parts	part	VERB
iajs-2699	68	8	product	product	NOUN
iajs-2699	68	9	of	of	ADP
iajs-2699	68	10	dna	dna	NOUN
iajs-2699	68	11	according	accord	VERB
iajs-2699	68	12	to	to	ADP
iajs-2699	68	13	their	their	PRON
iajs-2699	68	14	size	size	NOUN
iajs-2699	68	15	and	and	CCONJ
iajs-2699	68	16	charging	charging	NOUN
iajs-2699	68	17	,	,	PUNCT
iajs-2699	68	18	is	be	AUX
iajs-2699	68	19	usually	usually	ADV
iajs-2699	68	20	employed	employ	VERB
iajs-2699	68	21	technique	technique	NOUN
iajs-2699	68	22	for	for	ADP
iajs-2699	68	23	investigating	investigate	VERB
iajs-2699	68	24	the	the	DET
iajs-2699	68	25	pcr	pcr	ADJ
iajs-2699	68	26	product	product	NOUN
iajs-2699	68	27	.	.	PUNCT
iajs-2699	69	1	the	the	DET
iajs-2699	69	2	easiest	easy	ADJ
iajs-2699	69	3	way	way	NOUN
iajs-2699	69	4	to	to	PART
iajs-2699	69	5	view	view	VERB
iajs-2699	69	6	and	and	CCONJ
iajs-2699	69	7	analyze	analyze	VERB
iajs-2699	69	8	the	the	DET
iajs-2699	69	9	product	product	NOUN
iajs-2699	69	10	of	of	ADP
iajs-2699	69	11	pcr	pcr	NOUN
iajs-2699	69	12	is	be	AUX
iajs-2699	69	13	by	by	ADP
iajs-2699	69	14	using	use	VERB
iajs-2699	69	15	agarose	agarose	NOUN
iajs-2699	69	16	gel	gel	NOUN
iajs-2699	69	17	electrophoresis	electrophoresis	NOUN
iajs-2699	69	18	.	.	PUNCT
iajs-2699	70	1	it	it	PRON
iajs-2699	70	2	tolerates	tolerate	VERB
iajs-2699	70	3	the	the	DET
iajs-2699	70	4	existence	existence	NOUN
iajs-2699	70	5	and	and	CCONJ
iajs-2699	70	6	size	size	NOUN
iajs-2699	70	7	of	of	ADP
iajs-2699	70	8	the	the	DET
iajs-2699	70	9	product	product	NOUN
iajs-2699	70	10	of	of	ADP
iajs-2699	70	11	pcr	pcr	NOUN
iajs-2699	70	12	to	to	PART
iajs-2699	70	13	be	be	AUX
iajs-2699	70	14	resolved	resolve	VERB
iajs-2699	70	15	as	as	ADP
iajs-2699	70	16	depicted	depict	VERB
iajs-2699	70	17	(	(	PUNCT
iajs-2699	70	18	figure	figure	NOUN
iajs-2699	70	19	2	2	NUM
iajs-2699	70	20	)	)	PUNCT
iajs-2699	70	21	.	.	PUNCT
iajs-2699	71	1	a	a	DET
iajs-2699	71	2	determined	determined	ADJ
iajs-2699	71	3	set	set	NOUN
iajs-2699	71	4	of	of	ADP
iajs-2699	71	5	products	product	NOUN
iajs-2699	71	6	of	of	ADP
iajs-2699	71	7	pcr	pcr	NOUN
iajs-2699	71	8	with	with	ADP
iajs-2699	71	9	identified	identify	VERB
iajs-2699	71	10	sizes	size	NOUN
iajs-2699	71	11	have	have	AUX
iajs-2699	71	12	been	be	AUX
iajs-2699	71	13	run	run	VERB
iajs-2699	71	14	instantaneously	instantaneously	ADV
iajs-2699	71	15	on	on	ADP
iajs-2699	71	16	a	a	DET
iajs-2699	71	17	gel	gel	NOUN
iajs-2699	71	18	as	as	ADP
iajs-2699	71	19	typical	typical	ADJ
iajs-2699	71	20	molecular	molecular	ADJ
iajs-2699	71	21	indicators	indicator	NOUN
iajs-2699	71	22	for	for	ADP
iajs-2699	71	23	determining	determine	VERB
iajs-2699	71	24	the	the	DET
iajs-2699	71	25	product	product	NOUN
iajs-2699	71	26	size	size	NOUN
iajs-2699	71	27	.	.	PUNCT
iajs-2699	72	1	in	in	ADP
iajs-2699	72	2	general	general	ADJ
iajs-2699	72	3	,	,	PUNCT
iajs-2699	72	4	pcr	pcr	PROPN
iajs-2699	72	5	is	be	AUX
iajs-2699	72	6	referred	refer	VERB
iajs-2699	72	7	to	to	ADP
iajs-2699	72	8	as	as	SCONJ
iajs-2699	72	9	qualitative	qualitative	ADJ
iajs-2699	72	10	pcr	pcr	NOUN
iajs-2699	72	11	used	use	VERB
iajs-2699	72	12	to	to	PART
iajs-2699	72	13	distinguish	distinguish	VERB
iajs-2699	72	14	existence	existence	NOUN
iajs-2699	72	15	or	or	CCONJ
iajs-2699	72	16	lack	lack	NOUN
iajs-2699	72	17	of	of	ADP
iajs-2699	72	18	a	a	DET
iajs-2699	72	19	particular	particular	ADJ
iajs-2699	72	20	dna	dna	NOUN
iajs-2699	72	21	product	product	NOUN
iajs-2699	72	22	.	.	PUNCT
iajs-2699	73	1	pcr	pcr	PROPN
iajs-2699	73	2	is	be	AUX
iajs-2699	73	3	an	an	DET
iajs-2699	73	4	excellent	excellent	ADJ
iajs-2699	73	5	method	method	NOUN
iajs-2699	73	6	for	for	ADP
iajs-2699	73	7	cloning	cloning	NOUN
iajs-2699	73	8	and/or	and/or	CCONJ
iajs-2699	73	9	identifying	identify	VERB
iajs-2699	73	10	of	of	ADP
iajs-2699	73	11	a	a	DET
iajs-2699	73	12	pathogen	pathogen	NOUN
iajs-2699	73	13	when	when	SCONJ
iajs-2699	73	14	pcr	pcr	PROPN
iajs-2699	73	15	is	be	AUX
iajs-2699	73	16	performed	perform	VERB
iajs-2699	73	17	.	.	PUNCT
iajs-2699	74	1	for	for	ADP
iajs-2699	74	2	example	example	NOUN
iajs-2699	74	3	,	,	PUNCT
iajs-2699	74	4	merkel	merkel	PROPN
iajs-2699	74	5	cell	cell	NOUN
iajs-2699	74	6	polyomavirus	polyomavirus	NOUN
iajs-2699	74	7	exists	exist	VERB
iajs-2699	74	8	in	in	ADP
iajs-2699	74	9	the	the	DET
iajs-2699	74	10	skin	skin	NOUN
iajs-2699	74	11	-	-	PUNCT
iajs-2699	74	12	scale	scale	NOUN
iajs-2699	74	13	cell	cell	NOUN
iajs-2699	74	14	carcinoma	carcinoma	NOUN
iajs-2699	74	15	(	(	PUNCT
iajs-2699	74	16	scc	scc	NOUN
iajs-2699	74	17	)	)	PUNCT
iajs-2699	74	18	in	in	ADP
iajs-2699	74	19	immunocompetent	immunocompetent	ADJ
iajs-2699	74	20	individuals	individual	NOUN
iajs-2699	74	21	has	have	AUX
iajs-2699	74	22	been	be	AUX
iajs-2699	74	23	detected	detect	VERB
iajs-2699	74	24	through	through	ADP
iajs-2699	74	25	qualitative	qualitative	ADJ
iajs-2699	74	26	pcr	pcr	NOUN
iajs-2699	74	27	.	.	PUNCT
iajs-2699	75	1	ibn	ibn	PROPN
iajs-2699	75	2	al	al	PROPN
iajs-2699	75	3	-	-	PUNCT
iajs-2699	75	4	haitham	haitham	PROPN
iajs-2699	75	5	jour	jour	X
iajs-2699	75	6	.	.	PROPN
iajs-2699	75	7	for	for	ADP
iajs-2699	75	8	pure	pure	ADJ
iajs-2699	75	9	&	&	CCONJ
iajs-2699	75	10	appl	appl	PROPN
iajs-2699	75	11	.	.	PUNCT
iajs-2699	76	1	sci	sci	PROPN
iajs-2699	76	2	.	.	PROPN
iajs-2699	77	1	34(4)2021	34(4)2021	NUM
iajs-2699	77	2	39	39	NUM
iajs-2699	77	3	figure	figure	NOUN
iajs-2699	77	4	2	2	NUM
iajs-2699	77	5	:	:	PUNCT
iajs-2699	77	6	determining	determine	VERB
iajs-2699	77	7	existence	existence	NOUN
iajs-2699	77	8	and	and	CCONJ
iajs-2699	77	9	pcr	pcr	ADJ
iajs-2699	77	10	product	product	NOUN
iajs-2699	77	11	size	size	NOUN
iajs-2699	77	12	(	(	PUNCT
iajs-2699	77	13	dworkin	dworkin	PROPN
iajs-2699	77	14	et	et	PROPN
iajs-2699	77	15	al,2009	al,2009	PROPN
iajs-2699	77	16	)	)	PUNCT
iajs-2699	77	17	in	in	ADP
iajs-2699	77	18	general	general	ADJ
iajs-2699	77	19	,	,	PUNCT
iajs-2699	77	20	pcr	pcr	PROPN
iajs-2699	77	21	is	be	AUX
iajs-2699	77	22	referred	refer	VERB
iajs-2699	77	23	to	to	ADP
iajs-2699	77	24	as	as	SCONJ
iajs-2699	77	25	qualitative	qualitative	ADJ
iajs-2699	77	26	pcr	pcr	NOUN
iajs-2699	77	27	to	to	PART
iajs-2699	77	28	detect	detect	VERB
iajs-2699	77	29	the	the	DET
iajs-2699	77	30	existence	existence	NOUN
iajs-2699	77	31	or	or	CCONJ
iajs-2699	77	32	lack	lack	NOUN
iajs-2699	77	33	of	of	ADP
iajs-2699	77	34	a	a	DET
iajs-2699	77	35	particular	particular	ADJ
iajs-2699	77	36	dna	dna	NOUN
iajs-2699	77	37	product	product	NOUN
iajs-2699	77	38	.	.	PUNCT
iajs-2699	78	1	pcr	pcr	PROPN
iajs-2699	78	2	stands	stand	VERB
iajs-2699	78	3	for	for	ADP
iajs-2699	78	4	an	an	DET
iajs-2699	78	5	effective	effective	ADJ
iajs-2699	78	6	method	method	NOUN
iajs-2699	78	7	for	for	ADP
iajs-2699	78	8	cloning	cloning	NOUN
iajs-2699	78	9	and/or	and/or	CCONJ
iajs-2699	78	10	identifying	identify	VERB
iajs-2699	78	11	a	a	DET
iajs-2699	78	12	pathogen	pathogen	NOUN
iajs-2699	78	13	when	when	SCONJ
iajs-2699	78	14	pcr	pcr	PROPN
iajs-2699	78	15	is	be	AUX
iajs-2699	78	16	performed	perform	VERB
iajs-2699	78	17	.	.	PUNCT
iajs-2699	79	1	for	for	ADP
iajs-2699	79	2	instance	instance	NOUN
iajs-2699	79	3	,	,	PUNCT
iajs-2699	79	4	based	base	VERB
iajs-2699	79	5	on	on	ADP
iajs-2699	79	6	dworkin	dworkin	PROPN
iajs-2699	79	7	et	et	PROPN
iajs-2699	79	8	al	al	PROPN
iajs-2699	79	9	.	.	PROPN
iajs-2699	79	10	report	report	PROPN
iajs-2699	79	11	,	,	PUNCT
iajs-2699	79	12	the	the	DET
iajs-2699	79	13	existence	existence	NOUN
iajs-2699	79	14	of	of	ADP
iajs-2699	79	15	merkel	merkel	PROPN
iajs-2699	79	16	cell	cell	NOUN
iajs-2699	79	17	polyomavirus	polyomavirus	NOUN
iajs-2699	79	18	in	in	ADP
iajs-2699	79	19	skin	skin	NOUN
iajs-2699	79	20	squamous	squamous	ADJ
iajs-2699	79	21	cell	cell	NOUN
iajs-2699	79	22	carcinoma	carcinoma	NOUN
iajs-2699	79	23	(	(	PUNCT
iajs-2699	79	24	scc	scc	NOUN
iajs-2699	79	25	)	)	PUNCT
iajs-2699	79	26	in	in	ADP
iajs-2699	79	27	immunocompetent	immunocompetent	ADJ
iajs-2699	79	28	persons	person	NOUN
iajs-2699	79	29	was	be	AUX
iajs-2699	79	30	measured	measure	VERB
iajs-2699	79	31	by	by	ADP
iajs-2699	79	32	qualitative	qualitative	ADJ
iajs-2699	79	33	pcr	pcr	PROPN
iajs-2699	79	34	(	(	PUNCT
iajs-2699	79	35	dworkin	dworkin	PROPN
iajs-2699	79	36	et	et	PROPN
iajs-2699	79	37	al	al	PROPN
iajs-2699	79	38	,	,	PUNCT
iajs-2699	79	39	2009	2009	NUM
iajs-2699	79	40	)	)	PUNCT
iajs-2699	79	41	.	.	PUNCT
iajs-2699	80	1	by	by	ADP
iajs-2699	80	2	means	mean	NOUN
iajs-2699	80	3	of	of	ADP
iajs-2699	80	4	isolated	isolate	VERB
iajs-2699	80	5	genomic	genomic	NOUN
iajs-2699	80	6	dna	dna	NOUN
iajs-2699	80	7	from	from	ADP
iajs-2699	80	8	excised	excise	VERB
iajs-2699	80	9	sccs	sccs	NOUN
iajs-2699	80	10	from	from	ADP
iajs-2699	80	11	immunocompetent	immunocompetent	ADJ
iajs-2699	80	12	individuals	individual	NOUN
iajs-2699	80	13	and	and	CCONJ
iajs-2699	80	14	primers	primer	NOUN
iajs-2699	80	15	specific	specific	ADJ
iajs-2699	80	16	to	to	ADP
iajs-2699	80	17	virus	virus	NOUN
iajs-2699	80	18	genes	gene	NOUN
iajs-2699	80	19	.	.	PUNCT
iajs-2699	81	1	the	the	DET
iajs-2699	81	2	investigators	investigator	NOUN
iajs-2699	81	3	were	be	AUX
iajs-2699	81	4	capable	capable	ADJ
iajs-2699	81	5	of	of	ADP
iajs-2699	81	6	demonstrating	demonstrate	VERB
iajs-2699	81	7	an	an	DET
iajs-2699	81	8	existence	existence	NOUN
iajs-2699	81	9	of	of	ADP
iajs-2699	81	10	a	a	DET
iajs-2699	81	11	351base	351base	PROPN
iajs-2699	81	12	pair	pair	NOUN
iajs-2699	81	13	(	(	PUNCT
iajs-2699	81	14	bp	bp	PROPN
iajs-2699	81	15	)	)	PUNCT
iajs-2699	81	16	viral	viral	ADJ
iajs-2699	81	17	gene	gene	NOUN
iajs-2699	81	18	in	in	ADP
iajs-2699	81	19	six	six	NUM
iajs-2699	81	20	out	out	ADP
iajs-2699	81	21	of	of	ADP
iajs-2699	81	22	sixteen	sixteen	NUM
iajs-2699	81	23	tested	test	VERB
iajs-2699	81	24	samples	sample	NOUN
iajs-2699	81	25	,	,	PUNCT
iajs-2699	81	26	by	by	ADP
iajs-2699	81	27	the	the	DET
iajs-2699	81	28	existence	existence	NOUN
iajs-2699	81	29	of	of	ADP
iajs-2699	81	30	a	a	DET
iajs-2699	81	31	pcr	pcr	ADJ
iajs-2699	81	32	-	-	PUNCT
iajs-2699	81	33	product	product	NOUN
iajs-2699	81	34	band	band	NOUN
iajs-2699	81	35	of	of	ADP
iajs-2699	81	36	351	351	NUM
iajs-2699	81	37	bp	bp	PROPN
iajs-2699	81	38	long	long	ADV
iajs-2699	81	39	,	,	PUNCT
iajs-2699	81	40	as	as	SCONJ
iajs-2699	81	41	noted	note	VERB
iajs-2699	81	42	in	in	ADP
iajs-2699	81	43	figure-3	figure-3	NUM
iajs-2699	81	44	on	on	ADP
iajs-2699	81	45	the	the	DET
iajs-2699	81	46	2	2	NUM
iajs-2699	81	47	%	%	NOUN
iajs-2699	81	48	agarose	agarose	NOUN
iajs-2699	81	49	gel	gel	NOUN
iajs-2699	81	50	with	with	ADP
iajs-2699	81	51	ethidium	ethidium	NOUN
iajs-2699	81	52	bromide	bromide	NOUN
iajs-2699	81	53	depicted	depict	VERB
iajs-2699	81	54	.	.	PUNCT
iajs-2699	82	1	the	the	DET
iajs-2699	82	2	experimentation	experimentation	NOUN
iajs-2699	82	3	also	also	ADV
iajs-2699	82	4	involved	involve	VERB
iajs-2699	82	5	a	a	DET
iajs-2699	82	6	plasmid	plasmid	NOUN
iajs-2699	82	7	-	-	PUNCT
iajs-2699	82	8	containing	contain	VERB
iajs-2699	82	9	polyomavirus	polyomavirus	NOUN
iajs-2699	82	10	template	template	NOUN
iajs-2699	82	11	dna	dna	NOUN
iajs-2699	82	12	as	as	ADP
iajs-2699	82	13	a	a	DET
iajs-2699	82	14	positive	positive	ADJ
iajs-2699	82	15	(	(	PUNCT
iajs-2699	82	16	p	p	NOUN
iajs-2699	82	17	)	)	PUNCT
iajs-2699	82	18	and	and	CCONJ
iajs-2699	82	19	negative	negative	ADJ
iajs-2699	82	20	water	water	NOUN
iajs-2699	82	21	management	management	NOUN
iajs-2699	82	22	(	(	PUNCT
iajs-2699	82	23	w	w	NOUN
iajs-2699	82	24	)	)	PUNCT
iajs-2699	82	25	.	.	PUNCT
iajs-2699	83	1	a	a	DET
iajs-2699	83	2	foremost	foremost	ADJ
iajs-2699	83	3	(	(	PUNCT
iajs-2699	83	4	m	m	NOUN
iajs-2699	83	5	)	)	PUNCT
iajs-2699	83	6	lane	lane	NOUN
iajs-2699	83	7	is	be	AUX
iajs-2699	83	8	a	a	DET
iajs-2699	83	9	molecular	molecular	ADJ
iajs-2699	83	10	marker	marker	NOUN
iajs-2699	83	11	employed	employ	VERB
iajs-2699	83	12	to	to	PART
iajs-2699	83	13	recognize	recognize	VERB
iajs-2699	83	14	the	the	DET
iajs-2699	83	15	pcr	pcr	ADJ
iajs-2699	83	16	product	product	NOUN
iajs-2699	83	17	's	's	PART
iajs-2699	83	18	size	size	NOUN
iajs-2699	83	19	a	a	DET
iajs-2699	83	20	presence	presence	NOUN
iajs-2699	83	21	of	of	ADP
iajs-2699	83	22	a	a	DET
iajs-2699	83	23	pcr	pcr	NOUN
iajs-2699	83	24	-	-	PUNCT
iajs-2699	83	25	detected	detect	VERB
iajs-2699	83	26	viral	viral	ADJ
iajs-2699	83	27	particular	particular	ADJ
iajs-2699	83	28	gene	gene	NOUN
iajs-2699	83	29	has	have	AUX
iajs-2699	83	30	been	be	AUX
iajs-2699	83	31	characterized	characterize	VERB
iajs-2699	83	32	by	by	ADP
iajs-2699	83	33	(	(	PUNCT
iajs-2699	83	34	+	+	ADJ
iajs-2699	83	35	)	)	PUNCT
iajs-2699	83	36	;	;	PUNCT
iajs-2699	83	37	the	the	DET
iajs-2699	83	38	viral	viral	ADJ
iajs-2699	83	39	gene	gene	NOUN
iajs-2699	83	40	is	be	AUX
iajs-2699	83	41	not	not	PART
iajs-2699	83	42	present	present	ADJ
iajs-2699	83	43	by	by	ADP
iajs-2699	83	44	(	(	PUNCT
iajs-2699	83	45	−	−	NOUN
iajs-2699	83	46	)	)	PUNCT
iajs-2699	83	47	.	.	PUNCT
iajs-2699	84	1	figure-3.qualitative	figure-3.qualitative	ADJ
iajs-2699	84	2	pcr	pcr	PROPN
iajs-2699	84	3	distinguishes	distinguish	VERB
iajs-2699	84	4	merkel	merkel	PROPN
iajs-2699	84	5	cell	cell	NOUN
iajs-2699	84	6	polyomavirus	polyomavirus	PROPN
iajs-2699	84	7	existence	existence	NOUN
iajs-2699	84	8	in	in	ADP
iajs-2699	84	9	cutaneous	cutaneous	ADJ
iajs-2699	84	10	squamous	squamous	ADJ
iajs-2699	84	11	cell	cell	NOUN
iajs-2699	84	12	carcinoma	carcinoma	NOUN
iajs-2699	84	13	(	(	PUNCT
iajs-2699	84	14	scc	scc	NOUN
iajs-2699	84	15	)	)	PUNCT
iajs-2699	84	16	in	in	ADP
iajs-2699	84	17	immunocompetent	immunocompetent	ADJ
iajs-2699	84	18	individuals	individual	NOUN
iajs-2699	84	19	(	(	PUNCT
iajs-2699	84	20	drowkin	drowkin	PROPN
iajs-2699	84	21	et	et	PROPN
iajs-2699	84	22	al	al	PROPN
iajs-2699	84	23	,	,	PUNCT
iajs-2699	84	24	2009	2009	NUM
iajs-2699	84	25	)	)	PUNCT
iajs-2699	84	26	based	base	VERB
iajs-2699	84	27	on	on	ADP
iajs-2699	84	28	drowkin	drowkin	PROPN
iajs-2699	84	29	et	et	PROPN
iajs-2699	84	30	al	al	PROPN
iajs-2699	84	31	,	,	PUNCT
iajs-2699	84	32	2009	2009	NUM
iajs-2699	84	33	:	:	PUNCT
iajs-2699	84	34	mcpyv	mcpyv	ADV
iajs-2699	84	35	detecting	detecting	NOUN
iajs-2699	84	36	.	.	PUNCT
iajs-2699	85	1	(	(	PUNCT
iajs-2699	85	2	a	a	X
iajs-2699	85	3	)	)	PUNCT
iajs-2699	85	4	an	an	DET
iajs-2699	85	5	mcpyv	mcpyv	ADJ
iajs-2699	85	6	existence	existence	NOUN
iajs-2699	85	7	in	in	ADP
iajs-2699	85	8	normal	normal	ADJ
iajs-2699	85	9	genomic	genomic	NOUN
iajs-2699	85	10	dna	dna	NOUN
iajs-2699	85	11	,	,	PUNCT
iajs-2699	85	12	sccs	sccs	NOUN
iajs-2699	85	13	,	,	PUNCT
iajs-2699	85	14	and	and	CCONJ
iajs-2699	85	15	adjacent	adjacent	ADJ
iajs-2699	85	16	skin	skin	NOUN
iajs-2699	85	17	dna	dna	PROPN
iajs-2699	85	18	has	have	AUX
iajs-2699	85	19	been	be	AUX
iajs-2699	85	20	affected	affect	VERB
iajs-2699	85	21	through	through	ADP
iajs-2699	85	22	pcr	pcr	NOUN
iajs-2699	85	23	via	via	ADP
iajs-2699	85	24	vp1	vp1	NOUN
iajs-2699	85	25	https://www.ncbi.nlm.nih.gov/pmc/articles/pmc4102308/#r3	https://www.ncbi.nlm.nih.gov/pmc/articles/pmc4102308/#r3	NUM
iajs-2699	85	26	https://www.ncbi.nlm.nih.gov/pmc/articles/pmc4102308/figure/f3/	https://www.ncbi.nlm.nih.gov/pmc/articles/pmc4102308/figure/f3/	PROPN
iajs-2699	85	27	ibn	ibn	PROPN
iajs-2699	85	28	al	al	PROPN
iajs-2699	85	29	-	-	PUNCT
iajs-2699	85	30	haitham	haitham	PROPN
iajs-2699	85	31	jour	jour	X
iajs-2699	85	32	.	.	PROPN
iajs-2699	86	1	for	for	ADP
iajs-2699	86	2	pure	pure	ADJ
iajs-2699	86	3	&	&	CCONJ
iajs-2699	86	4	appl	appl	PROPN
iajs-2699	86	5	.	.	PUNCT
iajs-2699	87	1	sci	sci	PROPN
iajs-2699	87	2	.	.	PROPN
iajs-2699	88	1	34(4)2021	34(4)2021	NUM
iajs-2699	88	2	40	40	NUM
iajs-2699	88	3	primers	primer	NOUN
iajs-2699	88	4	.	.	PUNCT
iajs-2699	89	1	the	the	DET
iajs-2699	89	2	representative	representative	ADJ
iajs-2699	89	3	consequence	consequence	NOUN
iajs-2699	89	4	has	have	AUX
iajs-2699	89	5	been	be	AUX
iajs-2699	89	6	depicted	depict	VERB
iajs-2699	89	7	with	with	ADP
iajs-2699	89	8	6	6	NUM
iajs-2699	89	9	of	of	ADP
iajs-2699	89	10	16	16	NUM
iajs-2699	89	11	tested	test	VERB
iajs-2699	89	12	samples	sample	NOUN
iajs-2699	89	13	viewing	view	VERB
iajs-2699	89	14	a	a	DET
iajs-2699	89	15	pcr	pcr	ADJ
iajs-2699	89	16	product	product	NOUN
iajs-2699	89	17	at	at	ADP
iajs-2699	89	18	351	351	NUM
iajs-2699	89	19	bp	bp	NOUN
iajs-2699	89	20	.	.	PUNCT
iajs-2699	90	1	each	each	DET
iajs-2699	90	2	experimentation	experimentation	NOUN
iajs-2699	90	3	involved	involve	VERB
iajs-2699	90	4	dna	dna	NOUN
iajs-2699	90	5	from	from	ADP
iajs-2699	90	6	the	the	DET
iajs-2699	90	7	mcpyv	mcpyv	NOUN
iajs-2699	90	8	plasmid	plasmid	NOUN
iajs-2699	90	9	as	as	ADP
iajs-2699	90	10	a	a	DET
iajs-2699	90	11	positive	positive	ADJ
iajs-2699	90	12	control	control	NOUN
iajs-2699	90	13	(	(	PUNCT
iajs-2699	90	14	p	p	NOUN
iajs-2699	90	15	)	)	PUNCT
iajs-2699	90	16	and	and	CCONJ
iajs-2699	90	17	negative	negative	ADJ
iajs-2699	90	18	water	water	NOUN
iajs-2699	90	19	control	control	NOUN
iajs-2699	90	20	(	(	PUNCT
iajs-2699	90	21	w	w	NOUN
iajs-2699	90	22	)	)	PUNCT
iajs-2699	90	23	.	.	PUNCT
iajs-2699	91	1	m	m	PROPN
iajs-2699	91	2	,	,	PUNCT
iajs-2699	91	3	molecular	molecular	ADJ
iajs-2699	91	4	weight	weight	NOUN
iajs-2699	91	5	indicator	indicator	NOUN
iajs-2699	91	6	;	;	PUNCT
iajs-2699	92	1	+	+	ADJ
iajs-2699	92	2	,	,	PUNCT
iajs-2699	92	3	positive	positive	ADJ
iajs-2699	92	4	for	for	ADP
iajs-2699	92	5	virus	virus	NOUN
iajs-2699	92	6	;	;	PUNCT
iajs-2699	92	7	−	−	PROPN
iajs-2699	92	8	,	,	PUNCT
iajs-2699	92	9	negative	negative	ADJ
iajs-2699	92	10	for	for	ADP
iajs-2699	92	11	the	the	DET
iajs-2699	92	12	virus	virus	NOUN
iajs-2699	92	13	.	.	PUNCT
iajs-2699	93	1	5.pcr	5.pcr	ADV
iajs-2699	93	2	uses	use	VERB
iajs-2699	93	3	pcr	pcr	PROPN
iajs-2699	93	4	may	may	AUX
iajs-2699	93	5	be	be	AUX
iajs-2699	93	6	used	use	VERB
iajs-2699	93	7	to	to	PART
iajs-2699	93	8	diagnose	diagnose	VERB
iajs-2699	93	9	a	a	DET
iajs-2699	93	10	wide	wide	ADJ
iajs-2699	93	11	range	range	NOUN
iajs-2699	93	12	of	of	ADP
iajs-2699	93	13	human	human	ADJ
iajs-2699	93	14	diseases	disease	NOUN
iajs-2699	93	15	,	,	PUNCT
iajs-2699	93	16	experiments	experiment	NOUN
iajs-2699	93	17	,	,	PUNCT
iajs-2699	93	18	and	and	CCONJ
iajs-2699	93	19	analyzes	analyze	VERB
iajs-2699	93	20	[	[	PUNCT
iajs-2699	93	21	6,7	6,7	NUM
iajs-2699	93	22	]	]	PUNCT
iajs-2699	93	23	below	below	ADP
iajs-2699	93	24	are	be	AUX
iajs-2699	93	25	some	some	DET
iajs-2699	93	26	examples	example	NOUN
iajs-2699	93	27	.	.	PUNCT
iajs-2699	94	1	1	1	X
iajs-2699	94	2	.	.	X
iajs-2699	94	3	diseases	disease	NOUN
iajs-2699	94	4	of	of	ADP
iajs-2699	94	5	vih	vih	PROPN
iajs-2699	94	6	,	,	PUNCT
iajs-2699	94	7	cmv	cmv	NOUN
iajs-2699	94	8	,	,	PUNCT
iajs-2699	94	9	pneumonia	pneumonia	NOUN
iajs-2699	94	10	,	,	PUNCT
iajs-2699	94	11	hiv	hiv	PROPN
iajs-2699	94	12	,	,	PUNCT
iajs-2699	94	13	syphilis	syphilis	PROPN
iajs-2699	94	14	(	(	PUNCT
iajs-2699	94	15	mycoplasma	mycoplasma	PROPN
iajs-2699	94	16	)	)	PUNCT
iajs-2699	94	17	,	,	PUNCT
iajs-2699	94	18	msd	msd	PROPN
iajs-2699	94	19	,	,	PUNCT
iajs-2699	94	20	hiv	hiv	PROPN
iajs-2699	94	21	,	,	PUNCT
iajs-2699	94	22	hepatitis	hepatitis	NOUN
iajs-2699	94	23	,	,	PUNCT
iajs-2699	94	24	etc	etc	X
iajs-2699	94	25	.	.	X
iajs-2699	94	26	2	2	X
iajs-2699	94	27	.	.	X
iajs-2699	94	28	cancer	cancer	NOUN
iajs-2699	94	29	diagnosis	diagnosis	NOUN
iajs-2699	94	30	mainly	mainly	ADV
iajs-2699	94	31	for	for	ADP
iajs-2699	94	32	leukemia	leukemia	NOUN
iajs-2699	94	33	and	and	CCONJ
iajs-2699	94	34	lymphoma	lymphoma	NOUN
iajs-2699	94	35	3	3	NUM
iajs-2699	94	36	.	.	X
iajs-2699	94	37	paternity	paternity	NOUN
iajs-2699	94	38	test	test	NOUN
iajs-2699	94	39	genetic	genetic	ADJ
iajs-2699	94	40	fingerprinting	fingerprinting	NOUN
iajs-2699	94	41	.	.	PUNCT
iajs-2699	95	1	the	the	DET
iajs-2699	95	2	pcr	pcr	NOUN
iajs-2699	95	3	enables	enable	VERB
iajs-2699	95	4	early	early	ADJ
iajs-2699	95	5	diagnosis	diagnosis	NOUN
iajs-2699	95	6	of	of	ADP
iajs-2699	95	7	malignant	malignant	ADJ
iajs-2699	95	8	diseases	disease	NOUN
iajs-2699	95	9	as	as	ADP
iajs-2699	95	10	the	the	DET
iajs-2699	95	11	most	most	ADV
iajs-2699	95	12	advanced	advanced	ADJ
iajs-2699	95	13	diseases	disease	NOUN
iajs-2699	95	14	of	of	ADP
iajs-2699	95	15	cancer	cancer	NOUN
iajs-2699	95	16	research	research	NOUN
iajs-2699	95	17	currently	currently	ADV
iajs-2699	95	18	in	in	ADP
iajs-2699	95	19	use	use	NOUN
iajs-2699	95	20	,	,	PUNCT
iajs-2699	95	21	like	like	ADP
iajs-2699	95	22	leukemia	leukemia	NOUN
iajs-2699	95	23	and	and	CCONJ
iajs-2699	95	24	lymphomas	lymphoma	NOUN
iajs-2699	95	25	.	.	PUNCT
iajs-2699	96	1	to	to	PART
iajs-2699	96	2	identify	identify	VERB
iajs-2699	96	3	translocation	translocation	NOUN
iajs-2699	96	4	-	-	PUNCT
iajs-2699	96	5	specific	specific	ADJ
iajs-2699	96	6	malignant	malignant	ADJ
iajs-2699	96	7	cells	cell	NOUN
iajs-2699	96	8	,	,	PUNCT
iajs-2699	96	9	pcr	pcr	NOUN
iajs-2699	96	10	tests	test	NOUN
iajs-2699	96	11	may	may	AUX
iajs-2699	96	12	be	be	AUX
iajs-2699	96	13	done	do	VERB
iajs-2699	96	14	directly	directly	ADV
iajs-2699	96	15	in	in	ADP
iajs-2699	96	16	genomic	genomic	ADJ
iajs-2699	96	17	dna	dna	NOUN
iajs-2699	96	18	samples	sample	NOUN
iajs-2699	96	19	at	at	ADV
iajs-2699	96	20	least	least	ADV
iajs-2699	96	21	10,000	10,000	NUM
iajs-2699	96	22	times	time	NOUN
iajs-2699	96	23	greater	great	ADJ
iajs-2699	96	24	than	than	ADP
iajs-2699	96	25	other	other	ADJ
iajs-2699	96	26	techniques	technique	NOUN
iajs-2699	96	27	.	.	PUNCT
iajs-2699	97	1	pcr	pcr	PROPN
iajs-2699	97	2	also	also	ADV
iajs-2699	97	3	,	,	PUNCT
iajs-2699	97	4	tolerates	tolerate	VERB
iajs-2699	97	5	the	the	DET
iajs-2699	97	6	identification	identification	NOUN
iajs-2699	97	7	of	of	ADP
iajs-2699	97	8	microorganisms	microorganism	NOUN
iajs-2699	97	9	that	that	PRON
iajs-2699	97	10	can	can	AUX
iajs-2699	97	11	not	not	PART
iajs-2699	97	12	be	be	AUX
iajs-2699	97	13	cultivated	cultivate	VERB
iajs-2699	97	14	or	or	CCONJ
iajs-2699	97	15	slow	slow	ADV
iajs-2699	97	16	-	-	PUNCT
iajs-2699	97	17	growing	grow	VERB
iajs-2699	97	18	,	,	PUNCT
iajs-2699	97	19	like	like	ADP
iajs-2699	97	20	anaerobic	anaerobic	ADJ
iajs-2699	97	21	bacteria	bacteria	NOUN
iajs-2699	97	22	,	,	PUNCT
iajs-2699	97	23	mycobacteria	mycobacterium	NOUN
iajs-2699	97	24	or	or	CCONJ
iajs-2699	97	25	tissues	tissue	NOUN
iajs-2699	97	26	,	,	PUNCT
iajs-2699	97	27	and	and	CCONJ
iajs-2699	97	28	animal	animal	NOUN
iajs-2699	97	29	model	model	NOUN
iajs-2699	97	30	viruses	virus	NOUN
iajs-2699	97	31	.	.	PUNCT
iajs-2699	98	1	pcr	pcr	ADJ
iajs-2699	98	2	diagnostics	diagnostic	NOUN
iajs-2699	98	3	in	in	ADP
iajs-2699	98	4	microbiology	microbiology	NOUN
iajs-2699	98	5	are	be	AUX
iajs-2699	98	6	based	base	VERB
iajs-2699	98	7	on	on	ADP
iajs-2699	98	8	detecting	detect	VERB
iajs-2699	98	9	infectious	infectious	ADJ
iajs-2699	98	10	agents	agent	NOUN
iajs-2699	98	11	and	and	CCONJ
iajs-2699	98	12	discrimination	discrimination	NOUN
iajs-2699	98	13	against	against	ADP
iajs-2699	98	14	non	non	ADJ
iajs-2699	98	15	-	-	ADJ
iajs-2699	98	16	pathogenic	pathogenic	ADJ
iajs-2699	98	17	agents	agent	NOUN
iajs-2699	98	18	by	by	ADP
iajs-2699	98	19	particular	particular	ADJ
iajs-2699	98	20	genes	gene	NOUN
iajs-2699	98	21	from	from	ADP
iajs-2699	98	22	pathogenic	pathogenic	ADJ
iajs-2699	98	23	strains	strain	NOUN
iajs-2699	98	24	.	.	PUNCT
iajs-2699	99	1	for	for	ADP
iajs-2699	99	2	amplifying	amplify	VERB
iajs-2699	99	3	a	a	DET
iajs-2699	99	4	short	short	ADJ
iajs-2699	99	5	,	,	PUNCT
iajs-2699	99	6	well	well	ADV
iajs-2699	99	7	-	-	PUNCT
iajs-2699	99	8	defined	define	VERB
iajs-2699	99	9	portion	portion	NOUN
iajs-2699	99	10	of	of	ADP
iajs-2699	99	11	the	the	DET
iajs-2699	99	12	dna	dna	PROPN
iajs-2699	99	13	stream	stream	NOUN
iajs-2699	99	14	,	,	PUNCT
iajs-2699	99	15	pcr	pcr	PROPN
iajs-2699	99	16	is	be	AUX
iajs-2699	99	17	used	use	VERB
iajs-2699	99	18	.	.	PUNCT
iajs-2699	100	1	it	it	PRON
iajs-2699	100	2	can	can	AUX
iajs-2699	100	3	either	either	CCONJ
iajs-2699	100	4	be	be	AUX
iajs-2699	100	5	a	a	DET
iajs-2699	100	6	single	single	ADJ
iajs-2699	100	7	gene	gene	NOUN
iajs-2699	100	8	or	or	CCONJ
iajs-2699	100	9	a	a	DET
iajs-2699	100	10	gene	gene	NOUN
iajs-2699	100	11	part	part	NOUN
iajs-2699	100	12	.	.	PUNCT
iajs-2699	101	1	the	the	DET
iajs-2699	101	2	process	process	NOUN
iajs-2699	101	3	of	of	ADP
iajs-2699	101	4	pcr	pcr	NOUN
iajs-2699	101	5	can	can	AUX
iajs-2699	101	6	only	only	ADV
iajs-2699	101	7	copy	copy	VERB
iajs-2699	101	8	short	short	ADJ
iajs-2699	101	9	dna	dna	PROPN
iajs-2699	101	10	fragments	fragment	NOUN
iajs-2699	101	11	,	,	PUNCT
iajs-2699	101	12	typically	typically	ADV
iajs-2699	101	13	fit	fit	ADJ
iajs-2699	101	14	for	for	ADP
iajs-2699	101	15	10	10	NUM
iajs-2699	101	16	kb	kb	NOUN
iajs-2699	101	17	as	as	SCONJ
iajs-2699	101	18	opposed	oppose	VERB
iajs-2699	101	19	to	to	ADP
iajs-2699	101	20	living	live	VERB
iajs-2699	101	21	organisms	organism	NOUN
iajs-2699	101	22	(	(	PUNCT
iajs-2699	101	23	kb	kb	PROPN
iajs-2699	101	24	represents	represent	VERB
iajs-2699	101	25	kilo	kilo	PROPN
iajs-2699	101	26	base	base	ADJ
iajs-2699	101	27	pairs	pair	NOUN
iajs-2699	101	28	)	)	PUNCT
iajs-2699	101	29	.	.	PUNCT
iajs-2699	102	1	some	some	DET
iajs-2699	102	2	approaches	approach	NOUN
iajs-2699	102	3	may	may	AUX
iajs-2699	102	4	copy	copy	VERB
iajs-2699	102	5	fragments	fragment	NOUN
iajs-2699	102	6	fit	fit	ADJ
iajs-2699	102	7	for	for	ADP
iajs-2699	102	8	40	40	NUM
iajs-2699	102	9	kb	kb	PROPN
iajs-2699	102	10	,	,	PUNCT
iajs-2699	102	11	much	much	ADV
iajs-2699	102	12	smaller	small	ADJ
iajs-2699	102	13	than	than	ADP
iajs-2699	102	14	a	a	DET
iajs-2699	102	15	eucaryotic	eucaryotic	ADJ
iajs-2699	102	16	cell	cell	NOUN
iajs-2699	102	17	's	's	PART
iajs-2699	102	18	chromosomal	chromosomal	ADJ
iajs-2699	102	19	dna	dna	NOUN
iajs-2699	102	20	–	–	PUNCT
iajs-2699	102	21	for	for	ADP
iajs-2699	102	22	example	example	NOUN
iajs-2699	102	23	a	a	DET
iajs-2699	102	24	human	human	ADJ
iajs-2699	102	25	cell	cell	NOUN
iajs-2699	102	26	has	have	VERB
iajs-2699	102	27	approximately	approximately	ADV
iajs-2699	102	28	3	3	NUM
iajs-2699	102	29	billion	billion	NUM
iajs-2699	102	30	basic	basic	ADJ
iajs-2699	102	31	pairs	pair	NOUN
iajs-2699	102	32	.	.	PUNCT
iajs-2699	103	1	6.application	6.application	NUM
iajs-2699	103	2	of	of	ADP
iajs-2699	103	3	pcr	pcr	ADJ
iajs-2699	103	4	and	and	CCONJ
iajs-2699	103	5	diseases	disease	NOUN
iajs-2699	103	6	diagnosis	diagnosis	NOUN
iajs-2699	103	7	:	:	PUNCT
iajs-2699	103	8	detecting	detect	VERB
iajs-2699	103	9	mycobacterium	mycobacterium	NOUN
iajs-2699	103	10	tuberculosis	tuberculosis	NOUN
iajs-2699	103	11	from	from	ADP
iajs-2699	103	12	the	the	DET
iajs-2699	103	13	dot	dot	NOUN
iajs-2699	103	14	corner	corner	NOUN
iajs-2699	103	15	of	of	ADP
iajs-2699	103	16	mymensingh	mymensingh	PROPN
iajs-2699	103	17	medical	medical	PROPN
iajs-2699	103	18	college	college	PROPN
iajs-2699	103	19	hospital	hospital	NOUN
iajs-2699	103	20	in	in	ADP
iajs-2699	103	21	100	100	NUM
iajs-2699	103	22	suspected	suspect	VERB
iajs-2699	103	23	cases	case	NOUN
iajs-2699	103	24	of	of	ADP
iajs-2699	103	25	tb	tb	NOUN
iajs-2699	103	26	.	.	PUNCT
iajs-2699	104	1	in	in	ADP
iajs-2699	104	2	all	all	DET
iajs-2699	104	3	strains	strain	NOUN
iajs-2699	104	4	of	of	ADP
iajs-2699	104	5	mycobacterium	mycobacterium	NOUN
iajs-2699	104	6	tuberculosis	tuberculosis	NOUN
iajs-2699	104	7	,	,	PUNCT
iajs-2699	104	8	pcr	pcr	PROPN
iajs-2699	104	9	has	have	AUX
iajs-2699	104	10	been	be	AUX
iajs-2699	104	11	carried	carry	VERB
iajs-2699	104	12	out	out	ADP
iajs-2699	104	13	with	with	ADP
iajs-2699	104	14	primers	primer	NOUN
iajs-2699	104	15	mtb1	mtb1	NOUN
iajs-2699	104	16	and	and	CCONJ
iajs-2699	104	17	mttb2	mttb2	NOUN
iajs-2699	104	18	based	base	VERB
iajs-2699	104	19	on	on	ADP
iajs-2699	104	20	is6110	is6110	PROPN
iajs-2699	104	21	sequences	sequence	NOUN
iajs-2699	104	22	of	of	ADP
iajs-2699	104	23	the	the	DET
iajs-2699	104	24	pcr	pcr	ADJ
iajs-2699	104	25	sensitivity	sensitivity	NOUN
iajs-2699	104	26	and	and	CCONJ
iajs-2699	104	27	was	be	AUX
iajs-2699	104	28	found	find	VERB
iajs-2699	104	29	to	to	PART
iajs-2699	104	30	be	be	AUX
iajs-2699	104	31	94,7	94,7	NUM
iajs-2699	104	32	%	%	NOUN
iajs-2699	104	33	and	and	CCONJ
iajs-2699	104	34	100	100	NUM
iajs-2699	104	35	%	%	NOUN
iajs-2699	104	36	,	,	PUNCT
iajs-2699	104	37	respectively	respectively	ADV
iajs-2699	104	38	,	,	PUNCT
iajs-2699	104	39	for	for	ADP
iajs-2699	104	40	sputum	sputum	NOUN
iajs-2699	104	41	samples	sample	NOUN
iajs-2699	105	1	[	[	X
iajs-2699	105	2	6	6	NUM
iajs-2699	105	3	]	]	PUNCT
iajs-2699	105	4	.	.	PUNCT
iajs-2699	106	1	one	one	NUM
iajs-2699	106	2	of	of	ADP
iajs-2699	106	3	the	the	DET
iajs-2699	106	4	studies	study	NOUN
iajs-2699	106	5	revealed	reveal	VERB
iajs-2699	106	6	that	that	SCONJ
iajs-2699	106	7	klebsiella	klebsiella	PROPN
iajs-2699	106	8	pneumonia	pneumonia	NOUN
iajs-2699	106	9	carbapenems	carbapenem	NOUN
iajs-2699	106	10	genes	gene	NOUN
iajs-2699	106	11	,	,	PUNCT
iajs-2699	106	12	which	which	PRON
iajs-2699	106	13	have	have	VERB
iajs-2699	106	14	high	high	ADJ
iajs-2699	106	15	analytical	analytical	ADJ
iajs-2699	106	16	sensitivity	sensitivity	NOUN
iajs-2699	106	17	and	and	CCONJ
iajs-2699	106	18	specificity	specificity	NOUN
iajs-2699	106	19	of	of	ADP
iajs-2699	106	20	multidrug	multidrug	NOUN
iajs-2699	106	21	resistance	resistance	NOUN
iajs-2699	106	22	,	,	PUNCT
iajs-2699	106	23	have	have	AUX
iajs-2699	106	24	been	be	AUX
iajs-2699	106	25	accurately	accurately	ADV
iajs-2699	106	26	detected	detect	VERB
iajs-2699	106	27	in	in	ADP
iajs-2699	106	28	real	real	ADJ
iajs-2699	106	29	time	time	NOUN
iajs-2699	106	30	pcr	pcr	PROPN
iajs-2699	106	31	.	.	PUNCT
iajs-2699	107	1	according	accord	VERB
iajs-2699	107	2	to	to	ADP
iajs-2699	107	3	sybr	sybr	PROPN
iajs-2699	107	4	green	green	PROPN
iajs-2699	107	5	i	i	PROPN
iajs-2699	107	6	,	,	PUNCT
iajs-2699	107	7	a	a	DET
iajs-2699	107	8	real	real	ADJ
iajs-2699	107	9	-	-	PUNCT
iajs-2699	107	10	time	time	NOUN
iajs-2699	107	11	pcr	pcr	NOUN
iajs-2699	107	12	assay	assay	NOUN
iajs-2699	107	13	has	have	AUX
iajs-2699	107	14	been	be	AUX
iajs-2699	107	15	designed	design	VERB
iajs-2699	107	16	to	to	PART
iajs-2699	107	17	amplify	amplify	VERB
iajs-2699	107	18	a	a	DET
iajs-2699	107	19	106bp	106bp	ADJ
iajs-2699	107	20	product	product	NOUN
iajs-2699	107	21	for	for	ADP
iajs-2699	107	22	the	the	DET
iajs-2699	107	23	blakpc	blakpc	NOUN
iajs-2699	107	24	gene	gene	NOUN
iajs-2699	107	25	from	from	ADP
iajs-2699	107	26	159	159	NUM
iajs-2699	107	27	clinical	clinical	ADJ
iajs-2699	107	28	gram	gram	NOUN
iajs-2699	107	29	-ve	-ve	NOUN
iajs-2699	107	30	isolates	isolate	NOUN
iajs-2699	107	31	resistant	resistant	ADJ
iajs-2699	107	32	to	to	ADP
iajs-2699	107	33	numerous	numerous	ADJ
iajs-2699	107	34	kinds	kind	NOUN
iajs-2699	107	35	of	of	ADP
iajs-2699	107	36	lactam	lactam	NOUN
iajs-2699	107	37	antibiotics	antibiotic	NOUN
iajs-2699	107	38	.	.	PUNCT
iajs-2699	108	1	a	a	DET
iajs-2699	108	2	real	real	ADJ
iajs-2699	108	3	-	-	PUNCT
iajs-2699	108	4	time	time	NOUN
iajs-2699	108	5	pcr	pcr	NOUN
iajs-2699	108	6	detecting	detecting	NOUN
iajs-2699	108	7	limit	limit	NOUN
iajs-2699	108	8	has	have	AUX
iajs-2699	108	9	been	be	AUX
iajs-2699	108	10	approximately	approximately	ADV
iajs-2699	108	11	0.8cfu	0.8cfu	NUM
iajs-2699	108	12	using	use	VERB
iajs-2699	108	13	clinical	clinical	ADJ
iajs-2699	108	14	isolates	isolate	NOUN
iajs-2699	108	15	[	[	X
iajs-2699	108	16	8	8	NUM
iajs-2699	108	17	]	]	PUNCT
iajs-2699	108	18	.	.	PUNCT
iajs-2699	109	1	ibn	ibn	PROPN
iajs-2699	109	2	al	al	PROPN
iajs-2699	109	3	-	-	PUNCT
iajs-2699	109	4	haitham	haitham	PROPN
iajs-2699	109	5	jour	jour	X
iajs-2699	109	6	.	.	PROPN
iajs-2699	109	7	for	for	ADP
iajs-2699	109	8	pure	pure	ADJ
iajs-2699	109	9	&	&	CCONJ
iajs-2699	109	10	appl	appl	PROPN
iajs-2699	109	11	.	.	PUNCT
iajs-2699	110	1	sci	sci	PROPN
iajs-2699	110	2	.	.	PROPN
iajs-2699	111	1	34(4)2021	34(4)2021	NUM
iajs-2699	111	2	41	41	NUM
iajs-2699	111	3	pcr	pcr	ADJ
iajs-2699	111	4	diagnosis	diagnosis	NOUN
iajs-2699	111	5	has	have	AUX
iajs-2699	111	6	also	also	ADV
iajs-2699	111	7	,	,	PUNCT
iajs-2699	111	8	investigated	investigate	VERB
iajs-2699	111	9	in	in	ADP
iajs-2699	111	10	detecting	detect	VERB
iajs-2699	111	11	and	and	CCONJ
iajs-2699	111	12	differentiating	differentiate	VERB
iajs-2699	111	13	para	para	NOUN
iajs-2699	111	14	typhi	typhi	VERB
iajs-2699	111	15	,	,	PUNCT
iajs-2699	111	16	salmonella	salmonella	NOUN
iajs-2699	111	17	typhi	typhi	ADJ
iajs-2699	111	18	,	,	PUNCT
iajs-2699	111	19	brucellosis	brucellosis	NOUN
iajs-2699	111	20	,	,	PUNCT
iajs-2699	111	21	malaria	malaria	NOUN
iajs-2699	111	22	parasite	parasite	NOUN
iajs-2699	111	23	detection	detection	NOUN
iajs-2699	111	24	,	,	PUNCT
iajs-2699	111	25	dengue	dengue	NOUN
iajs-2699	111	26	infection	infection	NOUN
iajs-2699	111	27	,	,	PUNCT
iajs-2699	111	28	and	and	CCONJ
iajs-2699	111	29	h.pylori	h.pylori	NOUN
iajs-2699	111	30	in	in	ADP
iajs-2699	111	31	various	various	ADJ
iajs-2699	111	32	studies	study	NOUN
iajs-2699	111	33	all	all	ADV
iajs-2699	111	34	over	over	ADP
iajs-2699	111	35	the	the	DET
iajs-2699	111	36	world	world	NOUN
iajs-2699	111	37	.	.	PUNCT
iajs-2699	112	1	a	a	DET
iajs-2699	112	2	target	target	NOUN
iajs-2699	112	3	gene	gene	NOUN
iajs-2699	112	4	made	make	VERB
iajs-2699	112	5	amplicons	amplicon	NOUN
iajs-2699	112	6	at	at	ADP
iajs-2699	112	7	429bp	429bp	NOUN
iajs-2699	112	8	,	,	PUNCT
iajs-2699	112	9	330	330	NUM
iajs-2699	112	10	bp	bp	NOUN
iajs-2699	112	11	,	,	PUNCT
iajs-2699	112	12	and	and	CCONJ
iajs-2699	112	13	600bp	600bp	NOUN
iajs-2699	112	14	showing	show	VERB
iajs-2699	112	15	100	100	NUM
iajs-2699	112	16	%	%	NOUN
iajs-2699	112	17	sensitivity	sensitivity	NOUN
iajs-2699	112	18	for	for	ADP
iajs-2699	112	19	detecting	detect	VERB
iajs-2699	112	20	typhi	typhi	ADV
iajs-2699	112	21	,	,	PUNCT
iajs-2699	112	22	salmonella	salmonella	NOUN
iajs-2699	112	23	species	specie	NOUN
iajs-2699	112	24	,	,	PUNCT
iajs-2699	112	25	and	and	CCONJ
iajs-2699	112	26	paratyphi	paratyphi	VERB
iajs-2699	112	27	regarding	regard	VERB
iajs-2699	112	28	detecting	detect	VERB
iajs-2699	112	29	plasmodium	plasmodium	NOUN
iajs-2699	112	30	vivax	vivax	NOUN
iajs-2699	112	31	,	,	PUNCT
iajs-2699	112	32	the	the	DET
iajs-2699	112	33	+	+	NOUN
iajs-2699	112	34	ve	ve	VERB
iajs-2699	112	35	predictive	predictive	ADJ
iajs-2699	112	36	magnitudes	magnitude	NOUN
iajs-2699	112	37	of	of	ADP
iajs-2699	112	38	thick	thick	ADJ
iajs-2699	112	39	blood	blood	NOUN
iajs-2699	112	40	microscopy	microscopy	NOUN
iajs-2699	112	41	,	,	PUNCT
iajs-2699	112	42	nested	nested	ADJ
iajs-2699	112	43	pcr	pcr	NOUN
iajs-2699	112	44	,	,	PUNCT
iajs-2699	112	45	and	and	CCONJ
iajs-2699	112	46	real	real	ADJ
iajs-2699	112	47	-	-	PUNCT
iajs-2699	112	48	time	time	NOUN
iajs-2699	112	49	pcr	pcr	NOUN
iajs-2699	112	50	have	have	AUX
iajs-2699	112	51	been	be	AUX
iajs-2699	112	52	47.8	47.8	NUM
iajs-2699	112	53	%	%	NOUN
iajs-2699	112	54	,	,	PUNCT
iajs-2699	112	55	56.5	56.5	NUM
iajs-2699	112	56	%	%	NOUN
iajs-2699	112	57	,	,	PUNCT
iajs-2699	112	58	and	and	CCONJ
iajs-2699	112	59	60.9	60.9	NUM
iajs-2699	112	60	%	%	NOUN
iajs-2699	112	61	.	.	PUNCT
iajs-2699	113	1	a	a	DET
iajs-2699	113	2	real	real	ADJ
iajs-2699	113	3	time	time	NOUN
iajs-2699	113	4	pcr	pcr	PROPN
iajs-2699	113	5	has	have	VERB
iajs-2699	113	6	75	75	NUM
iajs-2699	113	7	%	%	NOUN
iajs-2699	113	8	specificity	specificity	NOUN
iajs-2699	113	9	,	,	PUNCT
iajs-2699	113	10	and	and	CCONJ
iajs-2699	113	11	100	100	NUM
iajs-2699	113	12	%	%	NOUN
iajs-2699	113	13	sensitivity	sensitivity	NOUN
iajs-2699	113	14	,	,	PUNCT
iajs-2699	113	15	whereas	whereas	SCONJ
iajs-2699	113	16	nested	nested	ADJ
iajs-2699	113	17	pcr	pcr	NOUN
iajs-2699	113	18	has	have	AUX
iajs-2699	113	19	shown	show	VERB
iajs-2699	113	20	81.2	81.2	NUM
iajs-2699	113	21	and	and	CCONJ
iajs-2699	113	22	97.7	97.7	NUM
iajs-2699	113	23	%	%	NOUN
iajs-2699	113	24	,	,	PUNCT
iajs-2699	113	25	respectively	respectively	ADV
iajs-2699	113	26	[	[	X
iajs-2699	113	27	10	10	NUM
iajs-2699	113	28	]	]	PUNCT
iajs-2699	113	29	for	for	ADP
iajs-2699	113	30	diagnosing	diagnose	VERB
iajs-2699	113	31	brucellosis	brucellosis	NOUN
iajs-2699	113	32	,	,	PUNCT
iajs-2699	113	33	the	the	DET
iajs-2699	113	34	most	most	ADV
iajs-2699	113	35	common	common	ADJ
iajs-2699	113	36	zoonotic	zoonotic	ADJ
iajs-2699	113	37	disease	disease	NOUN
iajs-2699	113	38	,	,	PUNCT
iajs-2699	113	39	pcr	pcr	ADJ
iajs-2699	113	40	detection	detection	NOUN
iajs-2699	113	41	using	use	VERB
iajs-2699	113	42	primers	primer	NOUN
iajs-2699	113	43	b4	b4	NOUN
iajs-2699	113	44	/	/	SYM
iajs-2699	113	45	b5	b5	PROPN
iajs-2699	113	46	amplifies	amplify	VERB
iajs-2699	113	47	gene	gene	NOUN
iajs-2699	113	48	encoding	encode	VERB
iajs-2699	113	49	a	a	DET
iajs-2699	113	50	31kda	31kda	NOUN
iajs-2699	113	51	immunogenic	immunogenic	ADJ
iajs-2699	113	52	outer	outer	ADJ
iajs-2699	113	53	membrane	membrane	NOUN
iajs-2699	113	54	protein	protein	NOUN
iajs-2699	113	55	(	(	PUNCT
iajs-2699	113	56	bcsp31	bcsp31	ADV
iajs-2699	113	57	)	)	PUNCT
iajs-2699	113	58	showing	show	VERB
iajs-2699	113	59	51.3	51.3	NUM
iajs-2699	113	60	%	%	NOUN
iajs-2699	113	61	exact	exact	ADJ
iajs-2699	113	62	sensitivity	sensitivity	NOUN
iajs-2699	113	63	and	and	CCONJ
iajs-2699	113	64	specificity	specificity	NOUN
iajs-2699	113	65	of	of	ADP
iajs-2699	113	66	100	100	NUM
iajs-2699	113	67	and	and	CCONJ
iajs-2699	113	68	10	10	NUM
iajs-2699	113	69	,	,	PUNCT
iajs-2699	113	70	respectively	respectively	ADV
iajs-2699	113	71	.	.	PUNCT
iajs-2699	114	1	tests	test	NOUN
iajs-2699	114	2	of	of	ADP
iajs-2699	114	3	histopathological	histopathological	ADJ
iajs-2699	114	4	and	and	CCONJ
iajs-2699	114	5	urease	urease	VERB
iajs-2699	114	6	in	in	ADP
iajs-2699	114	7	biopsy	biopsy	NOUN
iajs-2699	114	8	samples	sample	NOUN
iajs-2699	114	9	occasionally	occasionally	ADV
iajs-2699	114	10	generate	generate	VERB
iajs-2699	114	11	incorrect	incorrect	ADJ
iajs-2699	114	12	negative	negative	ADJ
iajs-2699	114	13	consequences	consequence	NOUN
iajs-2699	114	14	.	.	PUNCT
iajs-2699	115	1	for	for	ADP
iajs-2699	115	2	comparison	comparison	NOUN
iajs-2699	115	3	,	,	PUNCT
iajs-2699	115	4	pcr	pcr	ADJ
iajs-2699	115	5	assays	assay	NOUN
iajs-2699	115	6	have	have	AUX
iajs-2699	115	7	been	be	AUX
iajs-2699	115	8	implemented	implement	VERB
iajs-2699	115	9	in	in	ADP
iajs-2699	115	10	each	each	DET
iajs-2699	115	11	positive	positive	ADJ
iajs-2699	115	12	control	control	NOUN
iajs-2699	115	13	for	for	ADP
iajs-2699	115	14	h	h	NOUN
iajs-2699	115	15	pylori	pylori	NOUN
iajs-2699	115	16	showing	show	VERB
iajs-2699	115	17	sensitivity	sensitivity	NOUN
iajs-2699	115	18	and	and	CCONJ
iajs-2699	115	19	specificity	specificity	NOUN
iajs-2699	115	20	of	of	ADP
iajs-2699	115	21	64	64	NUM
iajs-2699	115	22	%	%	NOUN
iajs-2699	115	23	and	and	CCONJ
iajs-2699	115	24	80	80	NUM
iajs-2699	115	25	%	%	NOUN
iajs-2699	115	26	using	use	VERB
iajs-2699	115	27	optimal	optimal	ADJ
iajs-2699	115	28	combination	combination	NOUN
iajs-2699	115	29	of	of	ADP
iajs-2699	115	30	16rrna+urea[11	16rrna+urea[11	NUM
iajs-2699	115	31	]	]	PUNCT
iajs-2699	115	32	a	a	DET
iajs-2699	115	33	major	major	ADJ
iajs-2699	115	34	tropical	tropical	ADJ
iajs-2699	115	35	threat	threat	NOUN
iajs-2699	115	36	is	be	AUX
iajs-2699	115	37	chagas	chagas	NOUN
iajs-2699	115	38	or	or	CCONJ
iajs-2699	115	39	treypanosoma	treypanosoma	VERB
iajs-2699	115	40	cruzi	cruzi	PROPN
iajs-2699	115	41	.	.	PUNCT
iajs-2699	116	1	priorities	priority	NOUN
iajs-2699	116	2	are	be	AUX
iajs-2699	116	3	mainly	mainly	ADV
iajs-2699	116	4	on	on	ADP
iajs-2699	116	5	accurately	accurately	ADV
iajs-2699	116	6	diagnosing	diagnose	VERB
iajs-2699	116	7	and	and	CCONJ
iajs-2699	116	8	parasitological	parasitological	ADJ
iajs-2699	116	9	response	response	NOUN
iajs-2699	116	10	to	to	ADP
iajs-2699	116	11	treatment	treatment	NOUN
iajs-2699	116	12	.	.	PUNCT
iajs-2699	117	1	pcr	pcr	ADJ
iajs-2699	117	2	detection	detection	NOUN
iajs-2699	117	3	was	be	AUX
iajs-2699	117	4	investigated	investigate	VERB
iajs-2699	117	5	usingsequential	usingsequential	ADJ
iajs-2699	117	6	dilutions	dilution	NOUN
iajs-2699	117	7	of	of	ADP
iajs-2699	117	8	purified	purified	ADJ
iajs-2699	117	9	dna	dna	NOUN
iajs-2699	117	10	stocks	stock	NOUN
iajs-2699	117	11	including	include	VERB
iajs-2699	117	12	human	human	ADJ
iajs-2699	117	13	blood	blood	NOUN
iajs-2699	117	14	spiked	spike	VERB
iajs-2699	117	15	with	with	ADP
iajs-2699	117	16	parasite	parasite	NOUN
iajs-2699	117	17	cells	cell	NOUN
iajs-2699	117	18	(	(	PUNCT
iajs-2699	117	19	setb	setb	NOUN
iajs-2699	117	20	)	)	PUNCT
iajs-2699	117	21	,	,	PUNCT
iajs-2699	117	22	trypanosoma	trypanosoma	PROPN
iajs-2699	117	23	cruzi	cruzi	PROPN
iajs-2699	117	24	discrete	discrete	ADJ
iajs-2699	117	25	typing	type	VERB
iajs-2699	117	26	units	unit	NOUN
iajs-2699	117	27	i	i	PRON
iajs-2699	117	28	,	,	PUNCT
iajs-2699	117	29	iv	iv	PROPN
iajs-2699	117	30	,	,	PUNCT
iajs-2699	117	31	vi	vi	PROPN
iajs-2699	117	32	(	(	PUNCT
iajs-2699	117	33	set	set	VERB
iajs-2699	117	34	a	a	PRON
iajs-2699	117	35	)	)	PUNCT
iajs-2699	117	36	,	,	PUNCT
iajs-2699	117	37	and	and	CCONJ
iajs-2699	117	38	guinadine	guinadine	VERB
iajs-2699	117	39	hydrochroloride	hydrochroloride	ADV
iajs-2699	117	40	edta	edta	NOUN
iajs-2699	117	41	blood	blood	NOUN
iajs-2699	117	42	samples	sample	NOUN
iajs-2699	117	43	from	from	ADP
iajs-2699	117	44	thirty	thirty	NUM
iajs-2699	117	45	-	-	PUNCT
iajs-2699	117	46	two	two	NUM
iajs-2699	117	47	seropositive	seropositive	ADJ
iajs-2699	117	48	and	and	CCONJ
iajs-2699	117	49	ten	ten	NUM
iajs-2699	117	50	seronegative	seronegative	ADJ
iajs-2699	117	51	controls	control	NOUN
iajs-2699	117	52	(	(	PUNCT
iajs-2699	117	53	set	set	VERB
iajs-2699	117	54	c	c	NOUN
iajs-2699	117	55	)	)	PUNCT
iajs-2699	117	56	run	run	VERB
iajs-2699	117	57	by	by	ADP
iajs-2699	117	58	diverse	diverse	ADJ
iajs-2699	117	59	pcr	pcr	ADJ
iajs-2699	117	60	approaches	approach	NOUN
iajs-2699	117	61	based	base	VERB
iajs-2699	117	62	on	on	ADP
iajs-2699	117	63	primers	primer	NOUN
iajs-2699	117	64	121/122	121/122	NUM
iajs-2699	117	65	.	.	PUNCT
iajs-2699	118	1	the	the	DET
iajs-2699	118	2	consequence	consequence	NOUN
iajs-2699	118	3	has	have	AUX
iajs-2699	118	4	given	give	VERB
iajs-2699	118	5	83.3%-94.4	83.3%-94.4	NUM
iajs-2699	118	6	%	%	NOUN
iajs-2699	118	7	sensitivity	sensitivity	NOUN
iajs-2699	118	8	and	and	CCONJ
iajs-2699	118	9	specificity	specificity	NOUN
iajs-2699	118	10	of	of	ADP
iajs-2699	118	11	85	85	NUM
iajs-2699	118	12	-	-	SYM
iajs-2699	118	13	95	95	NUM
iajs-2699	118	14	%	%	NOUN
iajs-2699	118	15	.	.	PUNCT
iajs-2699	119	1	this	this	PRON
iajs-2699	119	2	characterizes	characterize	VERB
iajs-2699	119	3	global	global	ADJ
iajs-2699	119	4	substantiation	substantiation	NOUN
iajs-2699	119	5	for	for	ADP
iajs-2699	119	6	pcr	pcr	NOUN
iajs-2699	119	7	techniques	technique	NOUN
iajs-2699	119	8	for	for	ADP
iajs-2699	119	9	detecting	detect	VERB
iajs-2699	119	10	trepanosoma	trepanosoma	NOUN
iajs-2699	119	11	cruzi	cruzi	PROPN
iajs-2699	119	12	in	in	ADP
iajs-2699	119	13	human	human	ADJ
iajs-2699	119	14	blood	blood	NOUN
iajs-2699	119	15	samples	sample	NOUN
iajs-2699	119	16	[	[	X
iajs-2699	119	17	12	12	NUM
iajs-2699	119	18	]	]	PUNCT
iajs-2699	119	19	.	.	PUNCT
iajs-2699	120	1	for	for	ADP
iajs-2699	120	2	diagnosing	diagnose	VERB
iajs-2699	120	3	scrub	scrub	NOUN
iajs-2699	120	4	typhus	typhus	NOUN
iajs-2699	120	5	,	,	PUNCT
iajs-2699	120	6	a	a	DET
iajs-2699	120	7	study	study	NOUN
iajs-2699	120	8	studied	study	VERB
iajs-2699	120	9	conventional	conventional	ADJ
iajs-2699	120	10	pcr	pcr	NOUN
iajs-2699	120	11	(	(	PUNCT
iajs-2699	120	12	c	c	NOUN
iajs-2699	120	13	-	-	PUNCT
iajs-2699	120	14	pcr	pcr	ADJ
iajs-2699	120	15	)	)	PUNCT
iajs-2699	120	16	performances	performance	NOUN
iajs-2699	120	17	,	,	PUNCT
iajs-2699	120	18	nested	nest	VERB
iajs-2699	120	19	pcr(n	pcr(n	PROPN
iajs-2699	120	20	-	-	PUNCT
iajs-2699	120	21	pcr	pcr	NOUN
iajs-2699	120	22	)	)	PUNCT
iajs-2699	120	23	,	,	PUNCT
iajs-2699	120	24	real	real	ADJ
iajs-2699	120	25	-	-	PUNCT
iajs-2699	120	26	time	time	NOUN
iajs-2699	120	27	quantitative	quantitative	ADJ
iajs-2699	120	28	pcr(q	pcr(q	ADJ
iajs-2699	120	29	-	-	ADJ
iajs-2699	120	30	pcr	pcr	ADJ
iajs-2699	120	31	)	)	PUNCT
iajs-2699	120	32	aiming	aim	VERB
iajs-2699	120	33	o.tsutsugamushi	o.tsutsugamushi	X
iajs-2699	120	34	specific	specific	ADJ
iajs-2699	120	35	47kda	47kda	NOUN
iajs-2699	120	36	gene	gene	NOUN
iajs-2699	120	37	.	.	PUNCT
iajs-2699	121	1	dual	dual	ADJ
iajs-2699	121	2	template	template	NOUN
iajs-2699	121	3	plasmid	plasmid	NOUN
iajs-2699	121	4	dna	dna	NOUN
iajs-2699	121	5	and	and	CCONJ
iajs-2699	121	6	genomic	genomic	ADJ
iajs-2699	121	7	dna	dna	NOUN
iajs-2699	121	8	from	from	ADP
iajs-2699	121	9	buffy	buffy	PROPN
iajs-2699	121	10	coat	coat	NOUN
iajs-2699	121	11	sample	sample	NOUN
iajs-2699	121	12	for	for	ADP
iajs-2699	121	13	one	one	NUM
iajs-2699	121	14	patient	patient	NOUN
iajs-2699	121	15	has	have	AUX
iajs-2699	121	16	been	be	AUX
iajs-2699	121	17	employed	employ	VERB
iajs-2699	121	18	.	.	PUNCT
iajs-2699	122	1	n	n	CCONJ
iajs-2699	122	2	-	-	PUNCT
iajs-2699	122	3	pcr	pcr	ADJ
iajs-2699	122	4	,	,	PUNCT
iajs-2699	122	5	c	c	NOUN
iajs-2699	122	6	-	-	PUNCT
iajs-2699	122	7	pcr	pcr	ADJ
iajs-2699	122	8	,	,	PUNCT
iajs-2699	122	9	and	and	CCONJ
iajs-2699	122	10	q	q	ADJ
iajs-2699	122	11	pcr	pcr	ADJ
iajs-2699	122	12	sensitivities	sensitivity	NOUN
iajs-2699	122	13	have	have	AUX
iajs-2699	122	14	hown	hown	VERB
iajs-2699	122	15	with	with	ADP
iajs-2699	122	16	blood	blood	NOUN
iajs-2699	122	17	samples	sample	NOUN
iajs-2699	122	18	taken	take	VERB
iajs-2699	122	19	from	from	ADP
iajs-2699	122	20	patients	patient	NOUN
iajs-2699	122	21	with	with	ADP
iajs-2699	122	22	four	four	NUM
iajs-2699	122	23	weeks	week	NOUN
iajs-2699	122	24	for	for	ADP
iajs-2699	122	25	onset	onset	NOUN
iajs-2699	122	26	of	of	ADP
iajs-2699	122	27	fever	fever	NOUN
iajs-2699	122	28	have	have	AUX
iajs-2699	122	29	been	be	AUX
iajs-2699	122	30	7.3%,85.4	7.3%,85.4	NUM
iajs-2699	122	31	%	%	NOUN
iajs-2699	122	32	,	,	PUNCT
iajs-2699	122	33	and	and	CCONJ
iajs-2699	122	34	82.9%(95%ci)[13	82.9%(95%ci)[13	NUM
iajs-2699	122	35	]	]	X
iajs-2699	122	36	newly	newly	ADV
iajs-2699	122	37	diagnosing	diagnose	VERB
iajs-2699	122	38	pcr	pcr	NOUN
iajs-2699	122	39	was	be	AUX
iajs-2699	122	40	investigated	investigate	VERB
iajs-2699	122	41	in	in	ADP
iajs-2699	122	42	[	[	X
iajs-2699	122	43	14	14	NUM
iajs-2699	122	44	]	]	PUNCT
iajs-2699	122	45	in	in	ADP
iajs-2699	122	46	on	on	ADP
iajs-2699	122	47	samples	sample	NOUN
iajs-2699	122	48	from	from	ADP
iajs-2699	122	49	150	150	NUM
iajs-2699	122	50	healthy	healthy	ADJ
iajs-2699	122	51	volunteers	volunteer	NOUN
iajs-2699	122	52	and	and	CCONJ
iajs-2699	122	53	hospitalized	hospitalize	VERB
iajs-2699	122	54	adults	adult	NOUN
iajs-2699	122	55	.	.	PUNCT
iajs-2699	123	1	employing	employ	VERB
iajs-2699	123	2	culture	culture	NOUN
iajs-2699	123	3	as	as	ADP
iajs-2699	123	4	gold	gold	NOUN
iajs-2699	123	5	standard	standard	NOUN
iajs-2699	123	6	,	,	PUNCT
iajs-2699	123	7	the	the	DET
iajs-2699	123	8	pcr	pcr	ADJ
iajs-2699	123	9	sensitivity	sensitivity	NOUN
iajs-2699	123	10	and	and	CCONJ
iajs-2699	123	11	specificity	specificity	NOUN
iajs-2699	123	12	percentages	percentage	NOUN
iajs-2699	123	13	have	have	AUX
iajs-2699	123	14	been	be	AUX
iajs-2699	123	15	100	100	NUM
iajs-2699	123	16	%	%	NOUN
iajs-2699	123	17	and	and	CCONJ
iajs-2699	123	18	99.2	99.2	NUM
iajs-2699	123	19	%	%	NOUN
iajs-2699	123	20	respectively.[14	respectively.[14	PROPN
iajs-2699	123	21	]	]	PUNCT
iajs-2699	123	22	also	also	ADV
iajs-2699	123	23	,	,	PUNCT
iajs-2699	123	24	pcr	pcr	PROPN
iajs-2699	123	25	can	can	AUX
iajs-2699	123	26	detect	detect	VERB
iajs-2699	123	27	viral	viral	ADJ
iajs-2699	123	28	dna	dna	NOUN
iajs-2699	123	29	.	.	PUNCT
iajs-2699	124	1	the	the	DET
iajs-2699	124	2	primers	primer	NOUN
iajs-2699	124	3	employed	employ	VERB
iajs-2699	124	4	must	must	AUX
iajs-2699	124	5	be	be	AUX
iajs-2699	124	6	targeted	target	VERB
iajs-2699	124	7	in	in	ADP
iajs-2699	124	8	the	the	DET
iajs-2699	124	9	virus	virus	NOUN
iajs-2699	124	10	dna	dna	PROPN
iajs-2699	124	11	sequences	sequence	NOUN
iajs-2699	124	12	,	,	PUNCT
iajs-2699	124	13	and	and	CCONJ
iajs-2699	124	14	pcr	pcr	NOUN
iajs-2699	124	15	is	be	AUX
iajs-2699	124	16	feasibly	feasibly	ADV
iajs-2699	124	17	used	use	VERB
iajs-2699	124	18	or	or	CCONJ
iajs-2699	124	19	diagnostic	diagnostic	ADJ
iajs-2699	124	20	analysis	analysis	NOUN
iajs-2699	124	21	or	or	CCONJ
iajs-2699	124	22	viral	viral	ADJ
iajs-2699	124	23	genome	genome	NOUN
iajs-2699	124	24	dna	dna	NOUN
iajs-2699	124	25	sequences	sequence	NOUN
iajs-2699	124	26	.	.	PUNCT
iajs-2699	125	1	pcr	pcr	PROPN
iajs-2699	125	2	's	's	PART
iajs-2699	125	3	high	high	ADJ
iajs-2699	125	4	sensitivity	sensitivity	NOUN
iajs-2699	125	5	enables	enable	VERB
iajs-2699	125	6	the	the	DET
iajs-2699	125	7	virus	virus	NOUN
iajs-2699	125	8	to	to	PART
iajs-2699	125	9	be	be	AUX
iajs-2699	125	10	detected	detect	VERB
iajs-2699	125	11	immediately	immediately	ADV
iajs-2699	125	12	after	after	ADP
iajs-2699	125	13	and	and	CCONJ
iajs-2699	125	14	before	before	ADP
iajs-2699	125	15	the	the	DET
iajs-2699	125	16	infection	infection	NOUN
iajs-2699	125	17	.	.	PUNCT
iajs-2699	126	1	such	such	ADJ
iajs-2699	126	2	early	early	ADJ
iajs-2699	126	3	detection	detection	NOUN
iajs-2699	126	4	may	may	AUX
iajs-2699	126	5	lead	lead	VERB
iajs-2699	126	6	to	to	ADP
iajs-2699	126	7	significant	significant	ADJ
iajs-2699	126	8	treatment	treatment	NOUN
iajs-2699	126	9	leads	lead	VERB
iajs-2699	126	10	for	for	ADP
iajs-2699	126	11	doctors	doctor	NOUN
iajs-2699	126	12	.	.	PUNCT
iajs-2699	127	1	pcr	pcr	NOUN
iajs-2699	127	2	-	-	PUNCT
iajs-2699	127	3	based	base	VERB
iajs-2699	127	4	dna	dna	PROPN
iajs-2699	127	5	quantitation	quantitation	NOUN
iajs-2699	127	6	techniques	technique	NOUN
iajs-2699	127	7	can	can	AUX
iajs-2699	127	8	also	also	ADV
iajs-2699	127	9	quantify	quantify	VERB
iajs-2699	127	10	the	the	DET
iajs-2699	127	11	quantity	quantity	NOUN
iajs-2699	127	12	of	of	ADP
iajs-2699	127	13	viral	viral	ADJ
iajs-2699	127	14	load	load	NOUN
iajs-2699	127	15	in	in	ADP
iajs-2699	127	16	a	a	DET
iajs-2699	127	17	patient	patient	NOUN
iajs-2699	127	18	.	.	PUNCT
iajs-2699	127	19	"	"	PUNCT
iajs-2699	128	1	genetic	genetic	ADJ
iajs-2699	128	2	printing	printing	NOUN
iajs-2699	128	3	is	be	AUX
iajs-2699	128	4	the	the	DET
iajs-2699	128	5	technique	technique	NOUN
iajs-2699	128	6	of	of	ADP
iajs-2699	128	7	forensics	forensic	NOUN
iajs-2699	128	8	employed	employ	VERB
iajs-2699	128	9	to	to	PART
iajs-2699	128	10	categorize	categorize	VERB
iajs-2699	128	11	a	a	DET
iajs-2699	128	12	person	person	NOUN
iajs-2699	128	13	by	by	ADP
iajs-2699	128	14	ibn	ibn	PROPN
iajs-2699	128	15	al	al	PROPN
iajs-2699	128	16	-	-	PUNCT
iajs-2699	128	17	haitham	haitham	PROPN
iajs-2699	128	18	jour	jour	X
iajs-2699	128	19	.	.	PROPN
iajs-2699	128	20	for	for	ADP
iajs-2699	128	21	pure	pure	ADJ
iajs-2699	128	22	&	&	CCONJ
iajs-2699	128	23	appl	appl	PROPN
iajs-2699	128	24	.	.	PUNCT
iajs-2699	129	1	sci	sci	PROPN
iajs-2699	129	2	.	.	PROPN
iajs-2699	130	1	34(4)2021	34(4)2021	NUM
iajs-2699	130	2	42	42	NUM
iajs-2699	130	3	comparing	compare	VERB
iajs-2699	130	4	the	the	DET
iajs-2699	130	5	blood	blood	NOUN
iajs-2699	130	6	in	in	ADP
iajs-2699	130	7	a	a	DET
iajs-2699	130	8	crime	crime	NOUN
iajs-2699	130	9	script	script	NOUN
iajs-2699	130	10	with	with	ADP
iajs-2699	130	11	the	the	DET
iajs-2699	130	12	blood	blood	NOUN
iajs-2699	130	13	in	in	ADP
iajs-2699	130	14	a	a	DET
iajs-2699	130	15	suspect	suspect	NOUN
iajs-2699	130	16	's	's	PART
iajs-2699	130	17	dna	dna	NOUN
iajs-2699	130	18	to	to	ADP
iajs-2699	130	19	a	a	DET
iajs-2699	130	20	given	give	VERB
iajs-2699	130	21	sample	sample	NOUN
iajs-2699	130	22	[	[	X
iajs-2699	130	23	15,16,17	15,16,17	NUM
iajs-2699	130	24	]	]	PUNCT
iajs-2699	130	25	.	.	PUNCT
iajs-2699	131	1	7.pcr	7.pcr	ADJ
iajs-2699	131	2	advantages	advantage	NOUN
iajs-2699	131	3	pcr	pcr	NOUN
iajs-2699	131	4	is	be	AUX
iajs-2699	131	5	feasibly	feasibly	ADV
iajs-2699	131	6	employed	employ	VERB
iajs-2699	131	7	for	for	ADP
iajs-2699	131	8	diagnosing	diagnose	VERB
iajs-2699	131	9	numerous	numerous	ADJ
iajs-2699	131	10	human	human	ADJ
iajs-2699	131	11	diseases	disease	NOUN
iajs-2699	131	12	in	in	ADP
iajs-2699	131	13	various	various	ADJ
iajs-2699	131	14	experiments	experiment	NOUN
iajs-2699	131	15	and	and	CCONJ
iajs-2699	131	16	analyses	analysis	NOUN
iajs-2699	131	17	.	.	PUNCT
iajs-2699	132	1	"	"	PUNCT
iajs-2699	132	2	pcr	pcr	NOUN
iajs-2699	132	3	stands	stand	VERB
iajs-2699	132	4	for	for	ADP
iajs-2699	132	5	very	very	ADV
iajs-2699	132	6	imperative	imperative	ADJ
iajs-2699	132	7	confirmatory	confirmatory	NOUN
iajs-2699	132	8	diagnostic	diagnostic	ADJ
iajs-2699	132	9	aid	aid	NOUN
iajs-2699	132	10	in	in	ADP
iajs-2699	132	11	infectious	infectious	ADJ
iajs-2699	132	12	disease	disease	NOUN
iajs-2699	132	13	as	as	ADP
iajs-2699	132	14	in	in	ADP
iajs-2699	132	15	tuberculosis	tuberculosis	NOUN
iajs-2699	132	16	,	,	PUNCT
iajs-2699	132	17	syphilis	syphilis	PROPN
iajs-2699	132	18	,	,	PUNCT
iajs-2699	132	19	hiv	hiv	PROPN
iajs-2699	132	20	,	,	PUNCT
iajs-2699	132	21	cmv	cmv	NOUN
iajs-2699	132	22	,	,	PUNCT
iajs-2699	132	23	mycoplasma	mycoplasma	NOUN
iajs-2699	132	24	,	,	PUNCT
iajs-2699	132	25	fungous	fungous	ADJ
iajs-2699	132	26	and	and	CCONJ
iajs-2699	132	27	protozoal	protozoal	NOUN
iajs-2699	132	28	diseases	disease	NOUN
iajs-2699	132	29	,	,	PUNCT
iajs-2699	132	30	hepatitis	hepatitis	NOUN
iajs-2699	132	31	,	,	PUNCT
iajs-2699	132	32	cancers	cancer	NOUN
iajs-2699	132	33	particularly	particularly	ADV
iajs-2699	132	34	lymphoma	lymphoma	VERB
iajs-2699	132	35	malaria	malaria	NOUN
iajs-2699	132	36	and	and	CCONJ
iajs-2699	132	37	leukemia	leukemia	NOUN
iajs-2699	132	38	,	,	PUNCT
iajs-2699	132	39	tuberculosis	tuberculosis	NOUN
iajs-2699	132	40	,	,	PUNCT
iajs-2699	132	41	staphylococcal	staphylococcal	ADJ
iajs-2699	132	42	bacteremia	bacteremia	NOUN
iajs-2699	132	43	,	,	PUNCT
iajs-2699	132	44	toxoplasma	toxoplasma	NOUN
iajs-2699	132	45	gondoic	gondoic	NOUN
iajs-2699	133	1	[	[	X
iajs-2699	133	2	18	18	NUM
iajs-2699	133	3	,	,	PUNCT
iajs-2699	133	4	19	19	NUM
iajs-2699	133	5	,	,	PUNCT
iajs-2699	133	6	20	20	NUM
iajs-2699	133	7	]	]	PUNCT
iajs-2699	133	8	.	.	PUNCT
iajs-2699	134	1	"	"	PUNCT
iajs-2699	134	2	for	for	ADP
iajs-2699	134	3	the	the	DET
iajs-2699	134	4	fingers	finger	NOUN
iajs-2699	134	5	and	and	CCONJ
iajs-2699	134	6	paternity	paternity	NOUN
iajs-2699	134	7	test	test	NOUN
iajs-2699	134	8	,	,	PUNCT
iajs-2699	134	9	pcr	pcr	PROPN
iajs-2699	134	10	is	be	AUX
iajs-2699	134	11	also	also	ADV
iajs-2699	134	12	necessary	necessary	ADJ
iajs-2699	134	13	.	.	PUNCT
iajs-2699	135	1	high	high	ADJ
iajs-2699	135	2	sensitivity	sensitivity	NOUN
iajs-2699	135	3	and	and	CCONJ
iajs-2699	135	4	specificity	specificity	NOUN
iajs-2699	135	5	of	of	ADP
iajs-2699	135	6	pcr	pcr	NOUN
iajs-2699	135	7	(	(	PUNCT
iajs-2699	135	8	95	95	NUM
iajs-2699	135	9	-	-	SYM
iajs-2699	135	10	100	100	NUM
iajs-2699	135	11	percent	percent	NOUN
iajs-2699	135	12	)	)	PUNCT
iajs-2699	135	13	(	(	PUNCT
iajs-2699	135	14	100	100	NUM
iajs-2699	135	15	percent	percent	NOUN
iajs-2699	135	16	)	)	PUNCT
iajs-2699	136	1	[	[	X
iajs-2699	136	2	21,22	21,22	X
iajs-2699	136	3	]	]	X
iajs-2699	136	4	the	the	DET
iajs-2699	136	5	disadvantage	disadvantage	NOUN
iajs-2699	136	6	of	of	ADP
iajs-2699	136	7	pcr	pcr	NOUN
iajs-2699	136	8	*	*	PUNCT
iajs-2699	136	9	,	,	PUNCT
iajs-2699	136	10	it	it	PRON
iajs-2699	136	11	involves	involve	VERB
iajs-2699	136	12	pricey	pricey	ADJ
iajs-2699	136	13	instruments	instrument	NOUN
iajs-2699	136	14	such	such	ADJ
iajs-2699	136	15	as	as	ADP
iajs-2699	136	16	a	a	DET
iajs-2699	136	17	thermal	thermal	ADJ
iajs-2699	136	18	cycling	cycling	NOUN
iajs-2699	136	19	device	device	NOUN
iajs-2699	136	20	,	,	PUNCT
iajs-2699	136	21	a	a	DET
iajs-2699	136	22	diagnostic	diagnostic	ADJ
iajs-2699	136	23	tray	tray	NOUN
iajs-2699	136	24	of	of	ADP
iajs-2699	136	25	gar	gar	NOUN
iajs-2699	136	26	gels	gel	NOUN
iajs-2699	136	27	,	,	PUNCT
iajs-2699	136	28	a	a	DET
iajs-2699	136	29	dna	dna	PROPN
iajs-2699	136	30	separation	separation	NOUN
iajs-2699	136	31	kit	kit	PROPN
iajs-2699	136	32	,	,	PUNCT
iajs-2699	136	33	and	and	CCONJ
iajs-2699	136	34	other	other	ADJ
iajs-2699	136	35	chemical	chemical	ADJ
iajs-2699	136	36	agents	agent	NOUN
iajs-2699	136	37	that	that	PRON
iajs-2699	136	38	are	be	AUX
iajs-2699	136	39	unable	unable	ADJ
iajs-2699	136	40	to	to	PART
iajs-2699	136	41	buy	buy	VERB
iajs-2699	136	42	in	in	ADP
iajs-2699	136	43	all	all	DET
iajs-2699	136	44	laboratories	laboratory	NOUN
iajs-2699	136	45	.	.	PUNCT
iajs-2699	137	1	*	*	PUNCT
iajs-2699	137	2	adequate	adequate	ADJ
iajs-2699	137	3	air	air	NOUN
iajs-2699	137	4	conditioning	conditioning	NOUN
iajs-2699	137	5	space	space	NOUN
iajs-2699	137	6	,	,	PUNCT
iajs-2699	137	7	dehumidifier	dehumidifier	NOUN
iajs-2699	137	8	,	,	PUNCT
iajs-2699	137	9	laminar	laminar	ADJ
iajs-2699	137	10	flow	flow	NOUN
iajs-2699	137	11	equipment	equipment	NOUN
iajs-2699	137	12	,	,	PUNCT
iajs-2699	137	13	*	*	PUNCT
iajs-2699	137	14	maximum	maximum	ADJ
iajs-2699	137	15	scope	scope	NOUN
iajs-2699	137	16	of	of	ADP
iajs-2699	137	17	disease	disease	NOUN
iajs-2699	137	18	diagnosis	diagnosis	NOUN
iajs-2699	137	19	,	,	PUNCT
iajs-2699	137	20	*	*	PUNCT
iajs-2699	137	21	expensive	expensive	ADJ
iajs-2699	137	22	,	,	PUNCT
iajs-2699	137	23	not	not	PART
iajs-2699	137	24	everyone	everyone	PRON
iajs-2699	137	25	,	,	PUNCT
iajs-2699	137	26	*	*	PUNCT
iajs-2699	137	27	false	false	ADJ
iajs-2699	137	28	positive	positive	ADJ
iajs-2699	137	29	and	and	CCONJ
iajs-2699	137	30	false	false	ADJ
iajs-2699	137	31	negative	negative	ADJ
iajs-2699	137	32	results	result	NOUN
iajs-2699	137	33	could	could	AUX
iajs-2699	137	34	reduce	reduce	VERB
iajs-2699	137	35	specificities	specificity	NOUN
iajs-2699	137	36	and	and	CCONJ
iajs-2699	137	37	sensitivities	sensitivity	NOUN
iajs-2699	137	38	.	.	PUNCT
iajs-2699	138	1	8.benefits	8.benefits	NUM
iajs-2699	138	2	and	and	CCONJ
iajs-2699	138	3	drawbacks	drawback	NOUN
iajs-2699	138	4	of	of	ADP
iajs-2699	138	5	pcr	pcr	ADJ
iajs-2699	138	6	pcr	pcr	NOUN
iajs-2699	138	7	offers	offer	VERB
iajs-2699	138	8	some	some	DET
iajs-2699	138	9	benefits	benefit	NOUN
iajs-2699	138	10	.	.	PUNCT
iajs-2699	139	1	first	first	ADV
iajs-2699	139	2	,	,	PUNCT
iajs-2699	139	3	it	it	PRON
iajs-2699	139	4	is	be	AUX
iajs-2699	139	5	a	a	DET
iajs-2699	139	6	simple	simple	ADJ
iajs-2699	139	7	method	method	NOUN
iajs-2699	139	8	of	of	ADP
iajs-2699	139	9	understanding	understanding	NOUN
iajs-2699	139	10	and	and	CCONJ
iajs-2699	139	11	using	use	VERB
iajs-2699	139	12	quick	quick	ADJ
iajs-2699	139	13	results	result	NOUN
iajs-2699	139	14	(	(	PUNCT
iajs-2699	139	15	bolognia	bolognia	NOUN
iajs-2699	139	16	et	et	PROPN
iajs-2699	139	17	al	al	PROPN
iajs-2699	139	18	.	.	PROPN
iajs-2699	139	19	,	,	PUNCT
iajs-2699	139	20	2008	2008	NUM
iajs-2699	139	21	)	)	PUNCT
iajs-2699	139	22	.	.	PUNCT
iajs-2699	140	1	it	it	PRON
iajs-2699	140	2	has	have	VERB
iajs-2699	140	3	an	an	DET
iajs-2699	140	4	extremely	extremely	ADV
iajs-2699	140	5	sensitive	sensitive	ADJ
iajs-2699	140	6	technology	technology	NOUN
iajs-2699	140	7	with	with	ADP
iajs-2699	140	8	the	the	DET
iajs-2699	140	9	potential	potential	NOUN
iajs-2699	140	10	for	for	ADP
iajs-2699	140	11	sequencing	sequence	VERB
iajs-2699	140	12	,	,	PUNCT
iajs-2699	140	13	cloning	cloning	NOUN
iajs-2699	140	14	,	,	PUNCT
iajs-2699	140	15	and	and	CCONJ
iajs-2699	140	16	analyzing	analyze	VERB
iajs-2699	140	17	millions	million	NOUN
iajs-2699	140	18	or	or	CCONJ
iajs-2699	140	19	milliards	milliard	NOUN
iajs-2699	140	20	of	of	ADP
iajs-2699	140	21	copies	copy	NOUN
iajs-2699	140	22	of	of	ADP
iajs-2699	140	23	a	a	DET
iajs-2699	140	24	particular	particular	ADJ
iajs-2699	140	25	product	product	NOUN
iajs-2699	140	26	.	.	PUNCT
iajs-2699	141	1	this	this	PRON
iajs-2699	141	2	also	also	ADV
iajs-2699	141	3	applies	apply	VERB
iajs-2699	141	4	to	to	ADP
iajs-2699	141	5	qrt	qrt	NOUN
iajs-2699	141	6	-	-	PUNCT
iajs-2699	141	7	pcr	pcr	ADJ
iajs-2699	141	8	.	.	PUNCT
iajs-2699	142	1	the	the	DET
iajs-2699	142	2	quantification	quantification	NOUN
iajs-2699	142	3	of	of	ADP
iajs-2699	142	4	the	the	DET
iajs-2699	142	5	made	make	VERB
iajs-2699	142	6	product	product	NOUN
iajs-2699	142	7	has	have	VERB
iajs-2699	142	8	the	the	DET
iajs-2699	142	9	advantage	advantage	NOUN
iajs-2699	142	10	of	of	ADP
iajs-2699	142	11	qrt	qrt	NOUN
iajs-2699	142	12	-	-	PUNCT
iajs-2699	142	13	pcr	pcr	NOUN
iajs-2699	142	14	.	.	PUNCT
iajs-2699	143	1	it	it	PRON
iajs-2699	143	2	can	can	AUX
iajs-2699	143	3	thus	thus	ADV
iajs-2699	143	4	be	be	AUX
iajs-2699	143	5	used	use	VERB
iajs-2699	143	6	to	to	PART
iajs-2699	143	7	analyze	analyze	VERB
iajs-2699	143	8	changes	change	NOUN
iajs-2699	143	9	in	in	ADP
iajs-2699	143	10	tumor	tumor	NOUN
iajs-2699	143	11	,	,	PUNCT
iajs-2699	143	12	microbe	microbe	NOUN
iajs-2699	143	13	,	,	PUNCT
iajs-2699	143	14	or	or	CCONJ
iajs-2699	143	15	other	other	ADJ
iajs-2699	143	16	conditions	condition	NOUN
iajs-2699	143	17	of	of	ADP
iajs-2699	143	18	gene	gene	NOUN
iajs-2699	143	19	expression	expression	NOUN
iajs-2699	143	20	.	.	PUNCT
iajs-2699	144	1	pcr	pcr	PROPN
iajs-2699	144	2	is	be	AUX
iajs-2699	144	3	a	a	DET
iajs-2699	144	4	valuable	valuable	ADJ
iajs-2699	144	5	technique	technique	NOUN
iajs-2699	144	6	,	,	PUNCT
iajs-2699	144	7	but	but	CCONJ
iajs-2699	144	8	it	it	PRON
iajs-2699	144	9	is	be	AUX
iajs-2699	144	10	limited	limited	ADJ
iajs-2699	144	11	.	.	PUNCT
iajs-2699	145	1	due	due	ADP
iajs-2699	145	2	to	to	ADP
iajs-2699	145	3	the	the	DET
iajs-2699	145	4	high	high	ADJ
iajs-2699	145	5	sensitivity	sensitivity	NOUN
iajs-2699	145	6	of	of	ADP
iajs-2699	145	7	pcr	pcr	NOUN
iajs-2699	145	8	,	,	PUNCT
iajs-2699	145	9	any	any	DET
iajs-2699	145	10	form	form	NOUN
iajs-2699	145	11	of	of	ADP
iajs-2699	145	12	sample	sample	NOUN
iajs-2699	145	13	contamination	contamination	NOUN
iajs-2699	145	14	can	can	AUX
iajs-2699	145	15	produce	produce	VERB
iajs-2699	145	16	misleading	misleading	ADJ
iajs-2699	145	17	results	result	NOUN
iajs-2699	145	18	with	with	ADP
iajs-2699	145	19	even	even	ADV
iajs-2699	145	20	trace	trace	NOUN
iajs-2699	145	21	quantities	quantity	NOUN
iajs-2699	145	22	of	of	ADP
iajs-2699	145	23	dna	dna	PROPN
iajs-2699	145	24	[	[	X
iajs-2699	145	25	9,11	9,11	X
iajs-2699	145	26	]	]	PUNCT
iajs-2699	145	27	.	.	PUNCT
iajs-2699	146	1	furthermore	furthermore	ADV
iajs-2699	146	2	,	,	PUNCT
iajs-2699	146	3	certain	certain	ADJ
iajs-2699	146	4	previous	previous	ADJ
iajs-2699	146	5	sequence	sequence	NOUN
iajs-2699	146	6	data	datum	NOUN
iajs-2699	146	7	are	be	AUX
iajs-2699	146	8	necessary	necessary	ADJ
iajs-2699	146	9	for	for	ADP
iajs-2699	146	10	designing	design	VERB
iajs-2699	146	11	primers	primer	NOUN
iajs-2699	146	12	for	for	ADP
iajs-2699	146	13	pcr	pcr	NOUN
iajs-2699	146	14	.	.	PUNCT
iajs-2699	147	1	the	the	DET
iajs-2699	147	2	existence	existence	NOUN
iajs-2699	147	3	or	or	CCONJ
iajs-2699	147	4	nonexistence	nonexistence	NOUN
iajs-2699	147	5	of	of	ADP
iajs-2699	147	6	an	an	DET
iajs-2699	147	7	identified	identify	VERB
iajs-2699	147	8	pathogen	pathogen	NOUN
iajs-2699	147	9	or	or	CCONJ
iajs-2699	147	10	gene	gene	NOUN
iajs-2699	147	11	can	can	AUX
iajs-2699	147	12	therefore	therefore	ADV
iajs-2699	147	13	only	only	ADV
iajs-2699	147	14	be	be	AUX
iajs-2699	147	15	identified	identify	VERB
iajs-2699	147	16	by	by	ADP
iajs-2699	147	17	pcr	pcr	PROPN
iajs-2699	147	18	.	.	PUNCT
iajs-2699	148	1	a	a	DET
iajs-2699	148	2	further	further	ADJ
iajs-2699	148	3	restriction	restriction	NOUN
iajs-2699	148	4	is	be	AUX
iajs-2699	148	5	represented	represent	VERB
iajs-2699	148	6	by	by	ADP
iajs-2699	148	7	employed	employ	VERB
iajs-2699	148	8	primers	primer	NOUN
iajs-2699	148	9	for	for	ADP
iajs-2699	148	10	pcr	pcr	NOUN
iajs-2699	148	11	that	that	PRON
iajs-2699	148	12	can	can	AUX
iajs-2699	148	13	not	not	PART
iajs-2699	148	14	especially	especially	ADV
iajs-2699	148	15	be	be	AUX
iajs-2699	148	16	annealed	anneal	VERB
iajs-2699	148	17	to	to	PART
iajs-2699	148	18	not	not	PART
iajs-2699	148	19	identical	identical	VERB
iajs-2699	148	20	sequences	sequence	NOUN
iajs-2699	148	21	to	to	ADP
iajs-2699	148	22	the	the	DET
iajs-2699	148	23	target	target	NOUN
iajs-2699	148	24	dna	dna	NOUN
iajs-2699	148	25	but	but	CCONJ
iajs-2699	148	26	they	they	PRON
iajs-2699	148	27	are	be	AUX
iajs-2699	148	28	similar	similar	ADJ
iajs-2699	148	29	.	.	PUNCT
iajs-2699	149	1	furthermore	furthermore	ADV
iajs-2699	149	2	,	,	PUNCT
iajs-2699	149	3	dna	dna	NOUN
iajs-2699	149	4	polymerase	polymerase	NOUN
iajs-2699	149	5	can	can	AUX
iajs-2699	149	6	include	include	VERB
iajs-2699	149	7	improper	improper	ADJ
iajs-2699	149	8	nucleotides	nucleotide	NOUN
iajs-2699	149	9	into	into	ADP
iajs-2699	149	10	the	the	DET
iajs-2699	149	11	pcr	pcr	ADJ
iajs-2699	149	12	sequence	sequence	NOUN
iajs-2699	149	13	,	,	PUNCT
iajs-2699	149	14	albeit	albeit	SCONJ
iajs-2699	149	15	at	at	ADP
iajs-2699	149	16	a	a	DET
iajs-2699	149	17	highly	highly	ADV
iajs-2699	149	18	low	low	ADJ
iajs-2699	149	19	rate	rate	NOUN
iajs-2699	149	20	.	.	PUNCT
iajs-2699	150	1	9.conclusions	9.conclusions	NUM
iajs-2699	150	2	pcr	pcr	NOUN
iajs-2699	150	3	is	be	AUX
iajs-2699	150	4	a	a	DET
iajs-2699	150	5	simple	simple	ADJ
iajs-2699	150	6	and	and	CCONJ
iajs-2699	150	7	widely	widely	ADV
iajs-2699	150	8	used	use	VERB
iajs-2699	150	9	process	process	NOUN
iajs-2699	150	10	in	in	ADP
iajs-2699	150	11	which	which	PRON
iajs-2699	150	12	minute	minute	ADJ
iajs-2699	150	13	amounts	amount	NOUN
iajs-2699	150	14	of	of	ADP
iajs-2699	150	15	dna	dna	PROPN
iajs-2699	150	16	can	can	AUX
iajs-2699	150	17	be	be	AUX
iajs-2699	150	18	amplified	amplify	VERB
iajs-2699	150	19	into	into	ADP
iajs-2699	150	20	multiple	multiple	ADJ
iajs-2699	150	21	copies	copy	NOUN
iajs-2699	150	22	also	also	ADV
iajs-2699	150	23	,	,	PUNCT
iajs-2699	150	24	a	a	DET
iajs-2699	150	25	specific	specific	ADJ
iajs-2699	150	26	sequence	sequence	NOUN
iajs-2699	150	27	can	can	AUX
iajs-2699	150	28	be	be	AUX
iajs-2699	150	29	shown	show	VERB
iajs-2699	150	30	quantitatively	quantitatively	ADV
iajs-2699	150	31	with	with	ADP
iajs-2699	150	32	a	a	DET
iajs-2699	150	33	short	short	ADJ
iajs-2699	150	34	time	time	NOUN
iajs-2699	150	35	and	and	CCONJ
iajs-2699	150	36	accurate	accurate	ADJ
iajs-2699	150	37	results	result	NOUN
iajs-2699	150	38	for	for	ADP
iajs-2699	150	39	the	the	DET
iajs-2699	150	40	speed	speed	NOUN
iajs-2699	150	41	with	with	ADP
iajs-2699	150	42	which	which	PRON
iajs-2699	150	43	this	this	DET
iajs-2699	150	44	technique	technique	NOUN
iajs-2699	150	45	works	work	VERB
iajs-2699	150	46	.	.	PUNCT
iajs-2699	151	1	it	it	PRON
iajs-2699	151	2	is	be	AUX
iajs-2699	151	3	considered	consider	VERB
iajs-2699	151	4	an	an	DET
iajs-2699	151	5	accurate	accurate	ADJ
iajs-2699	151	6	diagnostic	diagnostic	ADJ
iajs-2699	151	7	method	method	NOUN
iajs-2699	151	8	for	for	ADP
iajs-2699	151	9	some	some	DET
iajs-2699	151	10	diseases	disease	NOUN
iajs-2699	151	11	and	and	CCONJ
iajs-2699	151	12	is	be	AUX
iajs-2699	151	13	characterized	characterize	VERB
iajs-2699	151	14	by	by	ADP
iajs-2699	151	15	high	high	ADJ
iajs-2699	151	16	sensitivity	sensitivity	NOUN
iajs-2699	151	17	and	and	CCONJ
iajs-2699	151	18	specificity	specificity	NOUN
iajs-2699	151	19	;	;	PUNCT
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iajs-2699	151	21	chain	chain	NOUN
iajs-2699	151	22	reaction	reaction	NOUN
iajs-2699	151	23	(	(	PUNCT
iajs-2699	151	24	pcr	pcr	PROPN
iajs-2699	151	25	)	)	PUNCT
iajs-2699	151	26	is	be	AUX
iajs-2699	151	27	a	a	DET
iajs-2699	151	28	very	very	ADV
iajs-2699	151	29	common	common	ADJ
iajs-2699	151	30	and	and	CCONJ
iajs-2699	151	31	widely	widely	ADV
iajs-2699	151	32	accepted	accept	VERB
iajs-2699	151	33	method	method	NOUN
iajs-2699	151	34	now	now	ADV
iajs-2699	151	35	all	all	ADV
iajs-2699	151	36	over	over	ADP
iajs-2699	151	37	the	the	DET
iajs-2699	151	38	world	world	NOUN
iajs-2699	151	39	references	reference	NOUN
iajs-2699	151	40	1	1	NUM
iajs-2699	151	41	.	.	PUNCT
iajs-2699	152	1	mullis	mullis	PROPN
iajs-2699	152	2	,	,	PUNCT
iajs-2699	152	3	k.b	k.b	PROPN
iajs-2699	152	4	.	.	PUNCT
iajs-2699	153	1	the	the	DET
iajs-2699	153	2	unusual	unusual	ADJ
iajs-2699	153	3	origin	origin	NOUN
iajs-2699	153	4	of	of	ADP
iajs-2699	153	5	the	the	DET
iajs-2699	153	6	polymerase	polymerase	NOUN
iajs-2699	153	7	chain	chain	NOUN
iajs-2699	153	8	reaction	reaction	NOUN
iajs-2699	153	9	.	.	PUNCT
iajs-2699	154	1	scientific	scientific	ADJ
iajs-2699	154	2	american	american	PROPN
iajs-2699	154	3	.	.	PROPN
iajs-2699	154	4	1990	1990	NUM
iajs-2699	154	5	,	,	PUNCT
iajs-2699	154	6	262(4	262(4	NUM
iajs-2699	154	7	)	)	PUNCT
iajs-2699	154	8	,	,	PUNCT
iajs-2699	154	9	56–61	56–61	NUM
iajs-2699	154	10	.	.	PUNCT
iajs-2699	155	1	[	[	X
iajs-2699	155	2	pubmed	pubmed	NOUN
iajs-2699	155	3	:	:	PUNCT
iajs-2699	155	4	2315679	2315679	NUM
iajs-2699	155	5	]	]	PUNCT
iajs-2699	155	6	doi	doi	NOUN
iajs-2699	155	7	:	:	PUNCT
iajs-2699	155	8	10.1038	10.1038	NUM
iajs-2699	155	9	/	/	SYM
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iajs-2699	155	11	-	-	SYM
iajs-2699	155	12	56	56	NUM
iajs-2699	155	13	.	.	NOUN
iajs-2699	155	14	2	2	NUM
iajs-2699	155	15	.	.	X
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iajs-2699	155	17	,	,	PUNCT
iajs-2699	155	18	j.	j.	PROPN
iajs-2699	155	19	m.	m.	PROPN
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iajs-2699	155	27	history	history	NOUN
iajs-2699	155	28	of	of	ADP
iajs-2699	155	29	the	the	DET
iajs-2699	155	30	polymerase	polymerase	NOUN
iajs-2699	155	31	chain	chain	NOUN
iajs-2699	155	32	reaction	reaction	NOUN
iajs-2699	155	33	.	.	PUNCT
iajs-2699	156	1	pcr	pcr	ADJ
iajs-2699	156	2	protocols	protocol	NOUN
iajs-2699	156	3	.	.	PUNCT
iajs-2699	157	1	2003	2003	NUM
iajs-2699	157	2	,	,	PUNCT
iajs-2699	157	3	226	226	NUM
iajs-2699	157	4	,	,	PUNCT
iajs-2699	157	5	3	3	NUM
iajs-2699	157	6	-	-	SYM
iajs-2699	157	7	6	6	NUM
iajs-2699	157	8	.	.	PUNCT
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iajs-2699	158	2	-	-	PUNCT
iajs-2699	158	3	59259	59259	NUM
iajs-2699	158	4	-	-	PUNCT
iajs-2699	158	5	384	384	NUM
iajs-2699	158	6	-	-	NUM
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iajs-2699	158	8	.	.	PUNCT
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iajs-2699	161	5	k.b	k.b	PROPN
iajs-2699	161	6	.	.	PUNCT
iajs-2699	162	1	the	the	DET
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iajs-2699	162	4	of	of	ADP
iajs-2699	162	5	the	the	DET
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iajs-2699	162	7	chain	chain	NOUN
iajs-2699	162	8	reaction	reaction	NOUN
iajs-2699	162	9	.	.	PUNCT
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iajs-2699	163	2	american	american	PROPN
iajs-2699	163	3	.	.	PROPN
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iajs-2699	163	8	,	,	PUNCT
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iajs-2699	164	2	.	.	PUNCT
iajs-2699	165	1	[	[	X
iajs-2699	165	2	pubmed	pubmed	NOUN
iajs-2699	165	3	:	:	PUNCT
iajs-2699	165	4	2315679	2315679	NUM
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iajs-2699	166	2	,	,	PUNCT
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iajs-2699	168	7	polymerase	polymerase	NOUN
iajs-2699	168	8	chain	chain	NOUN
iajs-2699	168	9	reaction	reaction	NOUN
iajs-2699	168	10	.	.	PUNCT
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iajs-2699	170	5	.	.	PUNCT
iajs-2699	171	1	[	[	X
iajs-2699	171	2	pubmed	pubmed	X
iajs-2699	171	3	:	:	PUNCT
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iajs-2699	171	7	:	:	PUNCT
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iajs-2699	173	2	,	,	PUNCT
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iajs-2699	173	4	)	)	PUNCT
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iajs-2699	173	7	.	.	PUNCT
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iajs-2699	174	2	pubmed	pubmed	X
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iajs-2699	174	22	;	;	PUNCT
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iajs-2699	176	4	)	)	PUNCT
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iajs-2699	176	7	.	.	PUNCT
iajs-2699	177	1	[	[	X
iajs-2699	177	2	pubmed	pubmed	X
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iajs-2699	178	3	m.a	m.a	PROPN
iajs-2699	178	4	.	.	PROPN
iajs-2699	178	5	;	;	PUNCT
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iajs-2699	178	14	-	-	PUNCT
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iajs-2699	179	5	.	.	PUNCT
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iajs-2699	180	2	,	,	PUNCT
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iajs-2699	180	6	)	)	PUNCT
iajs-2699	180	7	,	,	PUNCT
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iajs-2699	180	9	-	-	SYM
iajs-2699	180	10	9	9	NUM
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iajs-2699	181	3	:	:	PUNCT
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iajs-2699	182	9	;	;	PUNCT
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iajs-2699	182	12	,	,	PUNCT
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iajs-2699	185	1	[	[	X
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iajs-2699	185	3	:	:	PUNCT
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iajs-2699	185	18	,	,	PUNCT
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iajs-2699	185	21	jorizzo	jorizzo	PROPN
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iajs-2699	185	31	.	.	PUNCT
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iajs-2699	186	4	:	:	PUNCT
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iajs-2699	186	6	.	.	PUNCT
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iajs-2699	187	7	:	:	PUNCT
iajs-2699	188	1	mosby	mosby	PROPN
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iajs-2699	188	3	elsevier	elsevier	PROPN
iajs-2699	188	4	,	,	PUNCT
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iajs-2699	189	28	qpcr	qpcr	ADJ
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iajs-2699	189	31	in	in	ADP
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iajs-2699	189	34	.	.	PUNCT
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iajs-2699	190	3	.	.	PUNCT
iajs-2699	191	1	2011	2011	NUM
iajs-2699	191	2	,	,	PUNCT
iajs-2699	191	3	28(5	28(5	NUM
iajs-2699	191	4	)	)	PUNCT
iajs-2699	191	5	,	,	PUNCT
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iajs-2699	191	7	.	.	PUNCT
iajs-2699	192	1	[	[	X
iajs-2699	192	2	pubmed	pubmed	X
iajs-2699	192	3	:	:	PUNCT
iajs-2699	192	4	21569926	21569926	NUM
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iajs-2699	192	6	dol	dol	NOUN
iajs-2699	192	7	:	:	PUNCT
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iajs-2699	192	12	.	.	PUNCT
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iajs-2699	193	3	j.s	j.s	PROPN
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iajs-2699	193	14	pcr	pcr	NOUN
iajs-2699	193	15	(	(	PUNCT
iajs-2699	193	16	qpcr)-based	qpcr)-base	VERB
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iajs-2699	193	21	.	.	PUNCT
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iajs-2699	194	4	.	.	PUNCT
iajs-2699	195	1	2009	2009	NUM
iajs-2699	195	2	,	,	PUNCT
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iajs-2699	195	4	.	.	PUNCT
iajs-2699	196	1	[	[	X
iajs-2699	196	2	pubmed	pubmed	X
iajs-2699	196	3	:	:	PUNCT
iajs-2699	196	4	19120456	19120456	NUM
iajs-2699	196	5	]	]	PUNCT
iajs-2699	196	6	dol	dol	NOUN
iajs-2699	196	7	:	:	PUNCT
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iajs-2699	196	20	;	;	PUNCT
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iajs-2699	197	24	-	-	PUNCT
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iajs-2699	198	12	-	-	SYM
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iajs-2699	199	1	[	[	X
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iajs-2699	199	3	:	:	PUNCT
iajs-2699	199	4	22771614	22771614	NUM
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iajs-2699	199	6	.	.	PUNCT
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iajs-2699	200	2	.	.	X
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iajs-2699	204	6	-	-	PUNCT
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iajs-2699	205	17	,	,	PUNCT
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iajs-2699	206	10	.	.	PUNCT
iajs-2699	207	1	j	j	PROPN
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iajs-2699	207	4	.	.	PUNCT
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iajs-2699	208	3	49(11	49(11	PROPN
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iajs-2699	209	6	-	-	PUNCT
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iajs-2699	209	9	.	.	PUNCT
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iajs-2699	211	4	.	.	PUNCT
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iajs-2699	212	2	,	,	PUNCT
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iajs-2699	212	4	)	)	PUNCT
iajs-2699	212	5	,	,	PUNCT
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iajs-2699	212	7	-	-	SYM
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iajs-2699	212	10	:	:	PUNCT
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iajs-2699	212	17	.	.	PUNCT
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iajs-2699	213	7	;	;	PUNCT
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iajs-2699	213	9	,	,	PUNCT
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iajs-2699	213	11	-	-	PUNCT
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iajs-2699	213	17	-	-	PUNCT
iajs-2699	213	18	r.	r.	PROPN
iajs-2699	213	19	;	;	PUNCT
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iajs-2699	213	21	,	,	PUNCT
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iajs-2699	213	23	-	-	PUNCT
iajs-2699	213	24	ja	ja	PROPN
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iajs-2699	213	28	,	,	PUNCT
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iajs-2699	213	30	-	-	PUNCT
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iajs-2699	213	34	of	of	ADP
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iajs-2699	213	36	histolytica	histolytica	PROPN
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iajs-2699	213	38	in	in	ADP
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iajs-2699	213	40	samples	sample	NOUN
iajs-2699	213	41	.	.	PUNCT
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iajs-2699	214	2	j	j	PROPN
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iajs-2699	214	4	.	.	PUNCT
iajs-2699	215	1	2011	2011	NUM
iajs-2699	215	2	,	,	PUNCT
iajs-2699	215	3	49(3	49(3	PROPN
iajs-2699	215	4	)	)	PUNCT
iajs-2699	215	5	,	,	PUNCT
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iajs-2699	215	7	-	-	SYM
iajs-2699	215	8	84	84	NUM
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iajs-2699	215	10	:	:	PUNCT
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iajs-2699	215	12	/	/	SYM
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iajs-2699	215	14	.	.	PUNCT
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iajs-2699	216	11	.	.	PUNCT
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iajs-2699	217	2	.	.	PROPN
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iajs-2699	218	4	.	.	PUNCT
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iajs-2699	219	2	,	,	PUNCT
iajs-2699	219	3	m.s	m.s	PROPN
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iajs-2699	219	5	;	;	PUNCT
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iajs-2699	219	7	,	,	PUNCT
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iajs-2699	219	9	,	,	PUNCT
iajs-2699	219	10	n.	n.	NOUN
iajs-2699	219	11	;	;	PUNCT
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iajs-2699	219	13	,	,	PUNCT
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iajs-2699	219	15	;	;	PUNCT
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iajs-2699	219	17	,	,	PUNCT
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iajs-2699	219	22	,	,	PUNCT
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iajs-2699	219	39	;	;	PUNCT
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iajs-2699	219	41	,	,	PUNCT
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iajs-2699	220	4	,	,	PUNCT
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iajs-2699	220	6	,	,	PUNCT
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iajs-2699	220	8	:	:	PUNCT
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iajs-2699	220	10	-	-	SYM
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iajs-2699	220	12	-	-	SYM
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iajs-2699	220	14	-	-	SYM
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iajs-2699	220	17	.	.	PUNCT
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iajs-2699	223	2	,	,	PUNCT
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iajs-2699	223	4	)	)	PUNCT
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iajs-2699	224	6	-	-	SYM
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iajs-2699	224	9	.	.	PUNCT
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iajs-2699	226	35	.	.	PUNCT
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iajs-2699	228	2	,	,	PUNCT
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iajs-2699	228	4	)	)	PUNCT
iajs-2699	228	5	,	,	PUNCT
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iajs-2699	228	7	-	-	SYM
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iajs-2699	228	9	.	.	PUNCT
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iajs-2699	229	2	:	:	PUNCT
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iajs-2699	229	9	.	.	PUNCT
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iajs-2699	234	2	,	,	PUNCT
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iajs-2699	234	4	)	)	PUNCT
iajs-2699	234	5	,	,	PUNCT
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iajs-2699	234	9	.	.	PUNCT
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iajs-2699	235	2	:	:	PUNCT
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iajs-2699	235	4	/	/	SYM
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iajs-2699	235	6	-	-	SYM
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iajs-2699	235	9	.	.	PUNCT
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iajs-2699	239	4	)	)	PUNCT
iajs-2699	239	5	,	,	PUNCT
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iajs-2699	239	7	-	-	SYM
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