








key: cord-321412-6g3obmzc
authors: Khamis, Faryal; Al-Zakwani, Ibrahim; Al Hashmi, Sabria; Al Dowaiki, Samata; Al Bahrani, Maher; Pandak, Nenad; Al Khalili, Huda; Memish, Ziad
title: Therapeutic Plasma Exchange in Adults with Severe COVID-19 Infection
date: 2020-06-23
journal: Int J Infect Dis
DOI: 10.1016/j.ijid.2020.06.064
sha: 
doc_id: 321412
cord_uid: 6g3obmzc

OBJECTIVES: To evaluate the therapeutic use of plasma exchange in COVID-19 patients compared to controls. METHODS: Case series of critically ill adult men and non-pregnant women, ≥18 years of age, with laboratory confirmed COVID-19, was conducted at the Royal Hospital, Oman, from April 17(th) to May 11(th), 2020. Therapeutic plasma exchange (TPE) was performed on patients admitted to intensive care unit (ICU) with confirmed or imminent acute respiratory distress syndrome (ARDS) or severe pneumonia. Analysis was performed using univariate statistics. RESULTS: A total of 31 COVID-19 patients were included with an overall mean age of 51 ± 15 years (range: 27-76 years), 90% (n = 28) were males, and 35% (n = 11) of the patients had TPE as a mode of treatment. The TPE group was associated with higher extubation rates than the non-TPE cohort (73% versus 20%; p = 0.018). Additionally, patients on TPE had a lower 14 days (0 versus 35%; p = 0.033) and 28 days (0 versus 35%; p = 0.033) all-cause mortality compared to patients not on TPE. However, all-cause mortality was only marginally lower in the TPE group compared to the non-TPE group (9.1% versus 45%; p = 0.055; power = 66%). Laboratory and ventilatory parameters also improved with the TPE. CONCLUSIONS: The use of TPE in severe COVID-19 patients has been associated with improved outcomes, however, randomized controlled clinical trials are warranted to draw final conclusive findings.

The novel coronavirus ("SARS-CoV-2") pandemic continues to perpetuate globally with an estimated 7,930,989 cases and 433,783 pandemic by country and territory, 2020) deaths (as of June 15 th , 2020) without available effective treatment or vaccine.

Several randomized controlled clinical trials, in search of the cure, are in progress . The SARS-CoV-2 virus infects the respiratory epithelium of the lower airways, causing widespread damage via cytopathic effects, resulting in severe inflammation and pneumonitis. It is estimated that 13.8% of cases are severe and 6.1% are critical (WHO-China Joint Mission on Coronavirus Disease 2019 , 2020) that manifest as acute respiratory distress syndrome (ARDS), sepsis and /or multiorgan failure. The response to fulminant infection is characterized by excessive immune dysregulation (cytokine storm), inflammation, hypercoagulable state and endothelial dysfunction Chang, 2019) . Severe COVID-19 disease has been associated with lymphopenia and high levels of ferritin, C-reactive protein (CRP), lactate dehydrogenase (LDH), D-dimer and interleukin-6 (IL-6) (Xu et al., 2020) .

Recently, convalescent plasma containing protective antibodies, donated from survivors of COVID-19 infection, has been shown as promising and safe treatment (Joyner et al., 2020) .

Similarly, therapeutic plasma exchange (TPE), which is not a novel therapy, has been used in several studies for the management of severe infections such as 2009 HIN1 influenza A, sepsis and multiorgan failure with a trend towards improved survival (Knaup et al., 2018; Busund et al., 2002; Rimmer et al., 2014; Patel et al., 2012; . Moreover, TPE has been proposed as a possible supportive treatment for severe COVID-19 infection and has been shown to be effective in a few case reports (Shi et al., 2020; Zhang et al., 2020) .

We report here the results of TPE as a supportive/adjunct therapy for the management of COVID-19 ARDS and severe pneumonia.

The study was conducted at the Royal Hospital, a tertiary care hospital in Muscat, Oman, from April 17th, 2020 to May 11th, 2020. TPE was given after 7 and up to 14 days of illness to adult patients, 18 years of age, with laboratory confirmed COVID-19 disease who were admitted to intensive care unit (ICU) with confirmed or imminent respiratory failure and any one of the following conditions (ARDS Definition Task Force, 2012):

1. ARDS was defined as acute-onset hypoxemia (the ratio of the partial pressure of arterial oxygen to the fraction of inspired oxygen [Pao2:Fio2], <300) with >50% bilateral pulmonary opacities on chest imaging within 24 to 48 hours that were not fully explained by congestive heart failure.

J o u r n a l P r e -p r o o f 2. Severe pneumonia in adults was defined as fever or suspected respiratory infection plus one of the following: respiratory rate of >30 breaths/ minute, severe respiratory distress and SpO2 of <93% on room air.

3. Septic shock in adults was defined as: persisting hypotension despite volume resuscitation, requiring vasopressors to maintain mean arterial pressure of ≥65 mmHg and serum lactate level of <2 mmol/L. 4. Multiple organ dysfunction syndrome (MODS) was defined as the progressive, potentially reversible, dysfunction of two or more organ systems following acute, life-threatening disruption of systemic homeostasis.

We excluded pregnant women, patients with suspected or confirmed pulmonary embolism and patients with acute coronary syndrome.

The study was approved by the Royal Hospital Research and Ethics Committee (SRC#36/2020) and a written informed consent was obtained from the patient or (if intubated) through the health proxy.

Data collected included demographics, baseline characteristics, risk factors, sequential organ failure assessment (SOFA) score, respiratory parameters ( FIO2, PEEP, PO2/FIO2) pre plasma exchange (day 0) and post plasma exchange (day 7), laboratory parameters pre-plasma exchange (day 0) and post-plasma exchange (day 7), (absolute lymphocytic count (ALC), CRP, LDH, ferritin, D-dimer, IL-6, PH and lactate), radiological features and clinical outcomes including ICU length of stay, total length of stay, extubation and mortality rates at day 14 and day 28 post-plasma exchange as well as all-cause mortality. The data was compared with the medical records of 21 patients admitted from April 17 th to May 11 th , 2020 to the ICU with J o u r n a l P r e -p r o o f laboratory confirmed COVID-19 disease but did not receive any form of plasma therapy. The control group received the usual standard of care as per the National Guidelines (Ministry of Health, Oman. Human infection with novel corona virus (COVID-19)-7. Interim guideline for hospitals, primary care and private healthcare, 2020).

The control group was matched for baseline characteristics and severity of illness.

Description of exchange plasma procedure TPE was performed using Spectra Optia ® Apheresis System (TermuBact, Japan), a standard plasma exchange kit (Catalog number: 502058310220) and citrate dextrose solution, solution A (ACD-A) as an anticoagulant. Fresh frozen plasma (FFP) was used as a replacement solution. The total volume of plasma to be replaced was calculated as follows: plasma replacement (L) = body weight (kg) x (1/13) x (100-hematocrit). TPE was performed through a standard femoral central venous catheter (12 Fr). Each patient underwent a total of five procedures.

Descriptive statistics were used to describe the data. For categorical variables, frequencies and percentages were reported. Differences between groups were analyzed using Pearson's 2 tests (or Fisher's exact tests for expected cells of <5). For continuous variables, mean and standard deviation were used to summarize the data while analyses were performed using Student's t-test. Abnormally distributed variables were summarized using median and interquartile range and analyzed using Wilcoxon Mann-Whitney test. Statistical analyses were conducted using STATA version 16.1 (STATA Corporation, College Station, TX, USA).

The study enrolled a total of 31 COVID-19 patients fulfilling the WHO case definition (WHO Clinical management of severe acute respiratory infection when novel coronavirus (nCoV) infection is suspected: interim guidance, 25 January 2020, 2020) with an overall mean age of 51  15 years (range: 27-76 years) and 90% (n = 28) were males. A total of 35% (n = 11) of the patients had TPE as a mode of treatment. The three most prevalent comorbidities were diabetes mellitus (48%; n = 15), hypertension (39%; n = 12) and chronic kidney disease (13%; n = 4). Both the TPE and the control groups had similar baseline characteristics and underlying comorbidities. The overall median sequential organ failure assessment (SOFA) score was 3 (2 -6) for the control group and 6 (3-9) for the TPE group. Ninety one percent (n = 10) of the patients in the TPE group had either moderate [PaO2/FiO2 -200-100] or severe ARDS [PaO2/FiO2 -<100], while 50% (n = 10) in the control had either moderate or severe ARDS. A total of 50% (n =10) of the patients in the control group presented with severe pneumonia. Sixty-three percent (n = 19) of the patients had septic shock, 82% (n = 9) in TPE group and 53% (n = 10) in the control group, and 13% (n = 4) had MOD, 9.1% (n = 1) in the TPE group and 15% (n = 3) in the control group, respectively. There was a trend for those who were in the TPE group for worse SOFA scores and ARDS, however, the p-values were not statistically significant. Those on plasma exchange were less likely to be diagnosed with severe pneumonia than those not on plasma exchange (9.1% versus 50%; p = 0.047). Table 1 Table 2 outline the clinical outcome characteristics of the cohort stratified by TPE. Those on plasma exchange were associated with longer ICU length of stay compared to those that were not on plasma exchange (14 versus 6 days; p = 0.028), as they were more seriously ill.

Additionally, those on TPE were also more likely to be extubated than those not on plasma J o u r n a l P r e -p r o o f exchange (73% versus 20%; p = 0.018). Furthermore, patients on TPE had a lower 14 days (0 versus 35%; p = 0.033) and 28 days (0 versus 35%; p =0.033) mortality compared to patients not on TPE . However, all-cause mortality was only marginally lower in the plasma exchange group than those not on plasma exchange (9.1% versus 45%; p = 0.055; power = 66%).

Clinical indexes, laboratory findings and ventilatory parameters before and after plasma exchange are presented in Table 3 . Those on TPE generally showed reductions in SOFA scores, IL-6, CRP, D-dimer, and ferritin levels.

The novel coronavirus (SAR-CoV-2) has generated a worldwide attention due to the potential impact on global health. Presently, there are no definitive treatments for COVID-19 infection and current management strategies focus on supportive care, infection control and investigational therapies. Morbidity and mortality from COVID-19 infection has been linked to certain risk factors that include: age, prior lung disease, diabetes mellitus, cardiac disease, hypertension, and stroke Khamis et al., 2020) . The most prevalent risk factors in our patients were diabetes mellitus, hypertension and chronic renal disease.

Nearly most of the patients in the plasma group (10/11) presented with moderate to severe ARDS, while severe pneumonia was the main presentation in the control group. In comparison to the control group, the clinical outcomes were favourable in terms of extubation and mortality benefit with TPE and the effect on the mortality extended up to 28 days (0 vs 35% mortality, p = 0.033). There was also a tendency towards improvement in overall all-cause J o u r n a l P r e -p r o o f mortality, however the sample size was small as denoted by the study statistical power of only 66%.

The main causes of death in patients with COVID-19 infection were ARDS and cytokine storm syndrome (Felsensteina et al., 2020; Huang et al., 2020; Qin et al., 2020) . Additionally, 50% of patients presenting with cytokine storm syndrome usually develop ARDS (Chen et al., 2019) , thus early recognition and control of dysregulated immune reaction is essential. In severe COVID-19 infection, TPE removes toxins and deleterious inflammatory cytokines such as IL-1, IL-6, granulocyte-colony stimulating factor, tumor necrosis factor and other inflammatory parameters. These inflammatory mediators can trigger a cytokine storm mediated immune injury to the different target organs, resulting in capillary leak syndrome, progressive lung injury, respiratory failure and ARDS, shock, acute kidney injury and liver impairment (Seguin et al., 2016) .

Simultaneous replacement with normal or convalescent plasma helps to improve hypercoagulable state, reduce cytokine response and replaces ADAMTS13 enzyme (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13). The effect of TPE on the clinical and laboratory parameters was instantly observed following the completion of the 5 cycles (day 7). This included improvement in the SOFA scores, increase in the ALC and reduction in all inflammatory parameters such as CRP, D-dimer, ferritin and IL-6. This effect has also been reported elsewhere (Shi et al., 2020; Zhang et al., 2020) .

Just over half of our patients in the TPE group and one third in the control group received the IL-6 inhibitor; tocilizumab, after plasma exchange and this might additionally have contributed to the decrease in the cytokine storm. However, in a study by Xu and colleagues, the J o u r n a l P r e -p r o o f reduction in the inflammatory markers was instant even prior to receiving the IL-6 inhibitor (Xu et al., 2020) .

The use of TPE in the treatment of severe COVID-19 infection has shown some positive results, however, the benefit has been limited in macrophage activation syndrome, or sepsis complicated with MODS . Theoretically, TPE could also remove the formed SARS-CoV-2 antibodies in addition to the "harmful" dysregulated inflammatory mediators but it remains unclear if there is an antibody response during the cytokine storm or it develops subsequently. The current recommendation of the American Plasma Exchange Association in this regard is as follows "the effectiveness of plasma exchange has not yet been determined and should be individually selected" (Winters et al., 2020) .

In the implementation of plasma exchange, the key factor in its success is to start TPE in the early stages of COVID-19 inflammation and probably when proinflammatory cytokines are high (Yang et al., 2020) . In COVID-19 patients, it has been reported that the inflammatory cytokines released with severe diseases, including IL-6, were significantly higher around 7 to 14 days after the onset of the illness (Wan et al. 2020) . Thus, early initiation of TPE treatment, within this time frame, could be associated with better outcomes. In our patients, TPE was initiated from 7 days and up to 14 days of illness.

It is also necessary to administer TPE for the correct duration and volume, to monitor the potential drug removal of certain therapies such as immunomodulating agents and to practice the proper infection prevention and control measures. TPE is a tolerable procedure by most patients without the occurrence of adverse events, however, the procedure can be a challenge to perform in COVID-19 patients who are placed in prone position. Earlier studies of the use of plasma J o u r n a l P r e -p r o o f exchange in sepsis has showed that both the timing and disease severity are important for the beneficial effect of TPE (Yang et al., 2020) . We routinely performed five TPEs daily and noted that respiratory improvements were only seen after completing the therapy on day 7. Recognized adverse events related to TPE include allergic reactions, hypotension, hypocalcemia and line related infections. All patients in this case study tolerated TPE except for one episode of hypotension which resolved after normal saline bolus and hydrocortisone injection and did not recur with subsequent procedures.

We note that this single-center small trial is not without limitations. It's possible that patients could have improved naturally but the laboratory and ventilatory changes before and after plasma exchange are encouraging for the TPE group. The use of the IL-6 antagonist, tocilizumab, could also have contributed to the beneficial effects of TPE. The current study is a case series with small number of patients, a larger well-powered randomized clinical trial is warranted to confirm the beneficial outcomes of TPE.

This single-center, case series study on the use of TPE in the management of infection has demonstrated results that are encouraging and support the need for further investigations. Based on our experience, TPE should be utilized earlier in critically ill patients with MODS and ARDS within 7 to 14 days of onset of illness. TPE shows promise; however, randomized clinical trials are warranted to draw final conclusive findings.

The declare no conflict of interest J o u r n a l P r e -p r o o f J o u r n a l P r e -p r o o f

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IL-6, interleukin 6; CRP, C-reactive protein; CLL, chronic lymphocytic leukemia; FiO2, fraction of inspired oxygen; Extub, extubated



