3(1)81-87 CONTACT : Saroj Thapa Thapasaroj1998@gmail.com © International Journal of Applied Biology 81 Abstract Rice (Oryza sativa L.) is first staple crop of Nepal. The national average yield of rice is less than its potential yield, for which poor agronomic management has been reported as the critical factor. Among various agro-management practices seedling age, seeding density and nitrogen rates significantly affect the growth and yield of rice. The lower seeding density produces the taller plant, more effective tiller, lower sterility and higher grain yield. On the other hand, seedling of older age from higher seeding density gives the lowest yield. Transplanting younger seedling with low seeding density and application of recommended dose of nitrogen gives a higher yield. This article summarizes various effects of seedling age, seeding density and nitrogen rates on plant height, number of effective tillers, number of filled grains, thousand grain weight and grain yield of rice. This information may be useful for maize growers and researchers. ISSN : 2580-2410 eISSN : 2580-2119 Effect of seedling age, seeding density and nitrogen fertilizer on growth and grain yield of rice (Oryza sativa L.) Saroj Thapa1, Khagendra Thapa1, Jiban Shrestha2 & Amit Chaudhary1 1Agriculture and Forestry University, Rampur, Chitwan, Nepal 2Nepal Agricultural Research Council, Agriculture Botany Division, Khumaltar, Lalitpur, Nepal Introduction Rice (Oryza sativa L.) is considered as the most important staple food crop in Nepal, followed by maize (Zea mays L.) and wheat (Triticum aestivum L.) respectively. Rice is cultivated in wide range of altitude from plain of Terai to Jumla (3050 metre above sea level) of high hill region of Nepal (NARC, 2007). Total rice cultivated area in Nepal is 1.36 million ha with total production of 4.3 million ton (t) (CBS, 2015-16). The yield of rice in Nepal and world context are 3.16 and 4.36 t ha-1 respectively (FAOSTAT, 2012). Dependency of rainwater, low input use and poor crop management practices are the major causes of low rice yield in Nepal (AICC, 2018). Nepal has still very low productivity compared to other developed countries though rice is major cereal crop for Nepal and its high yielding varieties are already available in global market. There is huge gap between rice productivity at researcher level and farmer’s field. It is so because farmers are still unable to do proper nursery as well stand crop management. Padalia (1980) and Lal and Roy (1996) concluded seedling health determines the final yield of transplanted rice. Practices of proper nursery management, integrated nutrient and pest management, maintenance of proper seedling density and timely OPEN ACCESS International Journal of Applied Biology Keyword Rice Oryza sativa L. growth, grain yield, Nitrogen (N), Seedling age, seeding density. Article History Received 12 May 2019 Accepted 24 June 2019 International Journal of Applied Biology is licensed under a Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. International Journal of Applied Biology, p-ISSN : 2580-2410 e-ISSN : 2580-2119. Journal homepage : http://journal.unhas.ac.id/index.php/ijoab International Journal of Applied Biology, 3(1), 2019 82 transplantation of seedling can improve growth and development of rice plant (Naeem Sarwar, 2011; Subedi, 2013). Rainfed rice covers 79% proportion of total rice produced in Nepal. Among it, distribution of rainfed rice in lowland and upland area are 70 and 9 % respectively (CBS, 2015/16). Irregular and insufficient rainfall occurrence has delayed the transplantation date of seedling which has been reducing the final yield of rice (Mishra and Salokhe, 2008 ) as plant height, tiller production, panicle length, grain formation, grains per panicle, and other yield attributing characters are highly affected due to delayed transplantation of seedling (Ali et. al., 1995). Reduction of primary tiller occurs due to detoriation of buds in older seedlings as the consequence of delayed transplantation (Mobasser, 2007). Sufficient nutrient supply in rice nursery helps to enhance the dry weight, height, root density, root length and overall growth as well development of seedling (Singh, 2005). Proper growth and good final yield is obtained only after proper management of rice seedlings at their young stage and also using of healthy seedlings. Reduction in seedling mortality, enhancement in stress tolerating capacity, increased number of effective tillers etc. are possible as the result of optimum application of nitrogenous fertilizers in nursery time and using of good seedlings only. About 50% and 100% more dry matter accumulation have been observed in rice seedlings due to application of nitrogen and phosphorus in nursery (Ros et al., 1997). Maximum rice productivity (4.9 t ha-1) have been observed after the application of di-ammonium phosphate and single super phosphate @ 50 kg P2O5 ha -1 in nursery. It valued 21% more yield compared to control method (Rajagopalan and Krishnarajan, 1987). Practice of sufficient water supply, labour, weed management including other inputs that determines the optimum age of seedlings to be transplanted for better grain yield. In case of lowland field, seedlings of 25 to 50 days old are used for transplantation by farmers (Wagh, 1988). Previous researches has shown better yield when seedlings are transplanted before it gets 25 days old (Rao, 1987; Wagh, 1988). Researches has directed towards the yield performance of rice when transplanted under age of 20 days old although findings are still contradictory (NARC, 2004). Few researchers have studied the synergistic influence of seedbed management on the characteristics of younger/older seedlings and the consequent impact on plant growth and yield after transplanting. Thus the objectives of this study were to quantify the distinctive effects of nursery management and seedling age on yield and yield components of fine rice Materials and Methods Relevant literatures from 1980 to 2018 were collected from journal articles, books, dissertations and working papers on Effect of seedlings age, seeding density and nitrogen fertilizer on growth and grain yeld of rice. Web pages were visited and the relevant information was collected. Author's experience in agriculture intensification is also internalized in the paper. Finally, information collected from different sources were analyzed and presented. Results and Discussion Plant Height Both the environment as well as crop management practice effect the height of plant which is one of the major parameter determining growth status of plant. Nutrient management, health of young seedlings, crop field environment and genetic characteristics International Journal of Applied Biology, 3(1), 2019 83 also determine height of plant (Yoshida, 1981). Crop geometry of 20×15 cm found to be significantly increasing tiller number and plant height compared to spacing of 20× 10cm, 20×25 cm or 20×20 cm (Nayak et al.,2011). Transplantation of the younger seedling of 12 days old showed significantly taller plant height as compared to 30 days older seedlings in case of rice (Mishra and Salokhe, 2008). Similarly, compared to 21 days old seedlings, 7 and 14 days old younger seedlings produced significantly taller plant height of rice (Gani et al., 2002). Insufficient fertilizer application and maintaining higher seedling density in nursery significantly reduces plant height (Naeem Sarwar, 2011). Seedling health mainly depend upon nutrient management during its early growth stage (Mishra and Salokhe, 2008). Both the process of cell division and elongation are responsible in improvement of plant height. Significant increment in dry matter accumulation as the result of higher photo assimilates due to larger leaf area by the action of optimum utilization of nitrogenous fertilizer have been observed in rice plant (Chaturvedi, 2005; Zhilin et al., 1997). Longer root length, higher root density, lesser transplantation shock due to lesser leaf area and good health of younger seedlings are responsible for increasing cell division and elongation process which ultimately increases plant height (Sangsu et al., 1999; Rahman, 2001). Better seedlings used for transplantation showed significant enhancement of plant height (Farooq, 2007). Effective tillers Effective tiller number can be increased by using nitrogenous fertilizer (Budhar and Palaniappan, 1996). The combined practice of using N fertilizer and low seeding density during nursery management can increase the number of productive tiller of rice seedlings. On other hand, if nitrogenous fertilizer was not applied then the number of effective tiller found to be higher for high seeding density than low seedling density (Subedi, 2013). Generally, lower seeding density favours the number of effective tillers significantly (Sarwar, 2011). It has also been observed that in case of high seeding density, there were no tiller formation occurred as compared to low seeding density when 40 days old seedlings were used for transplantation. The age of seedling also effects the effective tiller number significantly. Comparison to younger seedlings, older seedlings have more number of productive tillers (Adhikari, 2013). Mobasser (2007) found that higher number of effective trillers (355 effective tillers m-2) was produced in case of 45 days older seedlings as compared to younger ones. If young seedlings of 12 days old are transplanted then higher number of effective tillers are produced independent to amount of nitrogenous fertilizer applied and seeding density maintained in nursery (Mishra and Salokhe, 2008). This result occurs due to proper growth and development of root, increased cell division and enlargement process which are the intermediary consequence of increased photo assimilate. These enhanced processes finally increases the height of plant as well as tiller density in rice (Shrirame et al., 2000; Vijayakumar et al., 2005; Salem et al., 2011). Number of filled grains The number of filled grains per panicle is also one of the essential yield attribute. The number of filled grains per panicle has been found to be significantly higher in case of low seeding density compared to medium seeding density but result was not significant compared to high seeding density. There was also non-significant difference between effect of medium and high seeding density on filled grain number per panicle. Comparison to transplanted older seedlings, younger seedling led to higher number of filled grains (Subedi, 2013). Highest number of filled grains produced in case of transplanted 45 days old seedlings among International Journal of Applied Biology, 3(1), 2019 84 transplanted 30, 60 and 75 days old seedlings (Khatun, 2002). Despite of significant effect of seedling age on filled grain number, there was no significant effect seen on amount of nitrogen fertilizer application done in nursery. Whereas, Mursal (2008) found that number of tiller per clump, seed weight and number of seed per panicle increased due to application of N fertilizer application. Huge or low amount of N application can lead to decreased filled grains number per panicle. The relation observed is that enormous amount of N utilization increases number of filled grains and also reduce number of unfilled grains. Thousand grain weight The 1000 grain weight being an essential yield attribute mainly depends upon genetic characteristic of that plant compared to environmental influence (Ashraf, 1999). Optimum utilization of fertilizer significantly enhances the 1000 grain weight value (Panda, 1991). Rice crop management with high seeding density, no nitrogen application and use of older seedling significantly reduces 1000 grain weight than compared to optimum management including practices of low seeding density, optimum N application and transplantation of younger seedlings (Saha, 1979; Sarwar, 2011).The low nitrogen application of @ 150 kg ha-1 produced significantly higher test weight than compared to higher nitrogen level of 200 kg ha-1. It may have happen due to excessive growth of rice plant creating quite large number of spikelet which caused deficiency of carbohydrates require to fill all the empty grains reducing dry matter accumulation. This finally reduced the test weight of rice crop (Pramanik and Bera, 2013; Channabasavanna and Setty, 1994; Raju and Reddy, 1993). Furthermore, Hasegawa et al. (1994) also supported the result of reduced dry matter accumulation due to application of high amount of nitrogen in rice. Grain Yield The factors including seeding density, N fertilizer application and seedling transplanting age have shown significant interactive effect on final grain yield of rice. Grain yield is reduced when practice of high seeding density and older seedlings transplantation with or without using N fertilizer are used. The amount and quality of grain can also be effected by types of nitrogenous fertilizer used for cultivation (Gately and Kelly, 1987). Urea is considered as cheaper nitrogenous fertilizer that also don’t have detrimental effect on amount and quality of rice grain yield. Gately et al. (1988) found that higher grain yield as the positive effect of calcium ammonium nitrate application which also have higher amount of N content compared to urea. Ebaid and Ghanem (2000) reported that application of nitrogen up to 144 kg ha-1 enhanced the growth of crop as well as its final grain yield too. El-Batal et al. (2004) found that plant height, panicle length, no. of filled grains panicle-1and grain yield significantly increased when nitrogen dose increased between 120 to 190 kg ha-1. Likewise, Yoseftabar (2013) higher plant growth, yield attributes and grain yield obtained as the result of nitrogen application done at the rate of 100, 200 and 300 kg ha-1 respectively. Comparison to practice of high seeding density, older seedlings, with or without nitrogen application has produced minimum grain yield (5.74 t ha-1) which is significantly lower compared to yield of 6096 t ha-1 obtained as the result of low seeding density, younger seedlings and with or without N application. Result found to be non-significant for low, medium and high seeding density in case of young seedling transplantation and without N application (Subedi, 2013). Higher competitive condition for plant growth and changes in pattern of growth are caused by late transplantation of seedling (Mandal, 1984). Higher development of plyllocron and higher effective tiller number per plant which finally increases International Journal of Applied Biology, 3(1), 2019 85 grin yield. This result is obtained by the transplantation as younger seedlings (De Datta, 1980). Moreover, younger seedlings of 20 days old maintaining low seeding density of 100 g m-2and with N application when transplanted in field produced maximum grain yield of 6.96 t ha-1 (Subedi, 2013). Conclusions The transplanting younger seedlings produces the maximum yield, irrespective of nursery management. The seedlings obtained from high seeding density should not be transplanted at older age because it reduced the yield of rice drastically. Transplanting younger seedling with low seeding density and application recommended dose of nitrogen gives higher yield References Adhikari, B., Mehera, B. & Haefele, S. 2013. Impact of Rice Nursery Nutrient Management, Seeding Density and Seedling Age on Yield and Yield Attributes. 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