Int. J. Aquat. Biol. (2021) 9(4): 244-247 ISSN: 2322-5270; P-ISSN: 2383-0956 Journal homepage: www.ij-aquaticbiology.com © 2021 Iranian Society of Ichthyology Short Communication Two new localities for Lobocheilos falcifer (Valenciennes, 1842) (Teleostei: Cyprinidae), a rare and vulnerable freshwater fish species of Java, Indonesia Veryl Hasan1,2, Lucas de Oliveira Vieira3,4, Felipe Polivanov Ottoni*3,4,1Endang Dewi Masithah5 1Aquaculture Department, Fisheries and Marine Faculty, Airlangga University, Surabaya, Indonesia. 2Generasi Biology Indonesia Foundation, Zoology Division, Gresik, Indonesia. 3Laboratory of Systematics and Ecology of Aquatic Organisms, Center of Agrarian and Environmental Sciences (CCAA), Federal University of Maranhão, Chapadinha-MA, Brazil. 4Federal University of Maranhão, Postgraduate Program in Environmental Sciences, Center of Agrarian and Environmental Sciences, Chapadinha-MA, Brazil. 5Marine Department, Fisheries and Marine Faculty, Airlangga University, Surabaya, Indonesia. Article history: Received 12 June 2021 Accepted 26 July 2021 Available online 2 5 August 2021 Keywords: Ahan, Endangered species, Labeoninae, Native species. Abstract: Lobocheilos falcifer is a rare and vulnerable freshwater fish species from Java, Indonesia and currently listed as Vulnerable (VU) within the IUCN Red List. In 2019, three specimens of L. falcifer were collected and photographed on Rawa Pening Lake, Semarang Regency, Central Java Province and Solo River, Karanganyar Regency, Central Java Province as two new localities for this species, extending its distribution in about 80 km to southeast. We also provided the morphological data of this species as well. Introduction Lobocheilos Bleeker, 1854 is a cyprinid endemic to Southeast Asia and the western Indonesia archipelago (Borneo, Java and Sumatra) (Rainboth, 1991; Ciccotto and Page, 2016; Ciccotto and Tan, 2018). Two species of Lobocheilos viz. L. lehat Bleeker, 1858 and L. falcifer (Valenciennes, 1842) occur in Java (Fricke et al., 2021), but few information on these species are available, especially on their distribution. Lobocheilos lehat is probably extinct (Fricke et al., 2021) considering that it is not sampled since it was first discovered on Parongkalong, West Java Province in 1853 (Kottelat et al., 1993; Kottelat and Tan, 2008), whereas L. falcifer is currently listed as a vulnerable species (VU) based on the IUCN Red List Status (Lombantobing, 2019). Lobocheilos falcifer was reported in the following freshwater localities in Java: Cisadane River, West Java Province (Kottelat and Tan, 2008; Hadiaty, 2011), Wadaslintang Reservoir, Central Java Province (Hasan et al., 2019a), and Tuntang River, Semarang Regency, Central Java Province (Hasan et al., 2019b). *Correspondence: Felipe Polivanov Ottoni DOI: https://doi.org/10.22034/ijab.v9i4.1285 E-mail: fpottoni@gmail.com In this paper, we reported new records of L. falcifer in the Rawa Pening Lake and Solo River, Central Java Province, a range extension of about 80 km southeast from the closest locality where the species was already known. Materials and Methods Three specimens of L. falcifer (Fig. 1) were collected using cast nets in 2019 in the outlet of the Rawa Pening Lake, and in the upstream of Solo River (Figs. 2, 3). These two sites are located in Semarang Regency and Karanganyar Regency, respectively, at Central Java Province, Indonesia. The specimens were labeled and fixed in 96% ethanol (Hasan et al., 2019). The material was deposited at the Zoology Laboratory, Generasi Biologi Indonesia Foundation (GBI). Morphological identification followed diagnostic characters presented in Kottelat and Tan (2008). Examined materials (All from Indonesia: Central Java Province): Lobocheilos falcifer: GBI0002, 1 male, 161 mm TL, Semarang Regency, Rawa Pening 245 Int. J. Aquat. Biol. (2021) 9(4): 244-247 Lake, outlet of the lake, 7°18'23"S; 110°26'37"E, V. Hasan, 10 Nov. 2019. — GBI0003, 1 female, 176 mm TL, Semarang Regency, Rawa Pening Lake, outlet of the lake, 7°18'23"S; 110°26'37"E, V. Hasan, 10 Nov. 2019. — GBI0004, 1 male, 168 mm TL, Karanganyar Regency, Solo River, upstream of the river, 7°47'06.0"S; 110°57'07.0"E, V. Hasan, 5 Dec. 2019. Results and Discussions Lobocheilos falcifer (Valenciennes, 1842) (Fig. 1) New records: See examined material section. Identification: Some morphometric and meristic data of the specimens examined here are presented in Table 1. Specimens collected on Rawa Pening Lake and Solo River (Fig. 1) were identified as L. falcifer based on the following diagnostic features proposed by Kottelat and Tan (2008), listed below. Lobocheilos falcifer is distinguished from the other species of Lobocheilos occurring in Borneo, Sumatra, Figure 1. Specimen of Lobocheilos falcifer GBI0002. A. lateral view of the body. B. lateral view of head. C. ventral view of head and position of the mouth (Photograph by V. Hasan). Figure 2. The locations where Lobocheilos. falcifer specimens were collected in this work. A. Rawa Pening Lake. B. Solo River; Central Java province, Java, Indonesia (Photograph by V. Hasan). 246 Hasan et al./ Two new localities for Lobocheilos falcifer and Java by the combination of the following features: no dark blotch on caudal peduncle; 30–31+2–3 lateral line scales; body uniformly silver and greenish on top of head to caudal peduncle in life and fresh specimens; faint mid–lateral strip in life and fresh specimens and all fins membranes colorless in life and fresh specimens. Our identification was confirmed by Tan Heok Hui (Lee Kong Chian Natural History Museum, Singapore). The discovery of L. falcifer from the Rawa Pening Lake and Solo River (Figs. 2, 3), both in Java, are new records for this rare and vulnerable species, extending its distribution in about 80 km to the southeast from the previous closest record (Fig. 3). There are several studies on freshwater fishes in Indonesia that are limited to a single or few rivers. In Sumatra, Kottelat and Tan (2008) recorded L. ixocheilos Kottelat & Tan, 2008 on Batang Hari Basin, and then Iqbal et al. (2017) L. ixocheilos on Musi Basin, being sampled in two localities separated by more than 150 km. New Table 1. Morphometric and meristic data of Lobocheilos falcifer. Morphometric data (mm) Present study Kottelat and Tan (2008) GBI0002 GBI0003 GBI0004 Total length 161.0 176.0 168.0 136.8 Standard length 134.0 143.0 137.0 115.1 Head length 25.0 25.9 25.5 25.8 Snout length 12.2 12.5 12.2 12.0 Pre dorsal length 50.4 60.0 50.7 44.7 Pre ventral length 60.2 60.5 60.3 51.3 Pre anal length 95.0 100.0 95.0 75.0 Meristic data Dorsal-fin rays 11 11 11 12 Pectoral-fin rays 16 17 16 17 Ventral-fin rays 9 9 9 9 Anal-fin rays 8 8 8 8 Caudal-fin rays 16 16 17 17 Figure 3. Distribution of Lobocheilos falcifer in Java. Black rhombus are the previous records of the species on Cisadane River (West Java Province), Wadaslintang Reservoir (Central Java provnce), and Tuntang River (Central Java Province). Black aquares are the new records of this study, on Rawa Pening Lake and Solo River (Central Java Province). 247 Int. J. Aquat. Biol. (2021) 9(4): 244-247 records of endemic species are an important contribution for the understanding of species diversity and biogeography, among other biological topics (Hasan and Islam, 2020; Hasan et al., 2021). Acknowledgments We thank Pedro Bragança (South African Institute for Aquatic Biodiversity, South Africa) for the English language review, Generasi Biologi Indonesia Foundation and Andik Setiawan as our guide; and the Ministry of Finance Indonesian as the Scholarship Program sponsor and for funding our research (no. 20160221035555). FPO thanks Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES - Finance Code 001), and Fundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão (FAPEMA) for providing the financial and infrastructure support to carry out his scientific research. 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