Int. J. Aquat. Biol. (2017) 5(6): 401-407 DOI: ISSN: 2322-5270; P-ISSN: 2383-0956 Journal homepage: www.ij-aquaticbiology.com © 2017 Iranian Society of Ichthyology Original Article A comparative study of parasite communities of some endemic fish species in the Alborz Dam and the Babol River in the Southern Caspian Sea basin, Mazandaran Province Ali Taheri Mirghaed*1, Maryam Barzegar1, Hoseinali Ebrahimzadeh Mousavi1, Hooman Rahmati-holasoo1, Abbas Bozorgnia2 1Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Iran. 2Faculty of Natural Resources Sciences, Ghaemshahr Branch, Islamic Azad University, Ghaemshahr, Iran. Article history: Received 10 September 2017 Accepted 2 December 2017 Available online 2 5 December 2017 Keywords: Caspian Sea Fish parasite Babol River Alborz Dam Abstract: The Babol River is one of the main rivers in the Southern Caspian Sea basin in Mazandaran Province. The Alborz Dam has been built along the river to provide annual irrigation and flood protection. This study aimed to identify and describe the prevalence and parasite intensity of some endemic fish species in Babol River and Alborz Dam and to compare their parasite communities. The field investigations were carried out from June 2016 to March 2017 and approximately, 546 fish specimens, including Alburnoides tabarestanensis, Capoeta razii and Squalius turcicus, were examined. A total of 13 parasite species, including Ichthyophthirius multifiliis, Trichodina gracilis, Myxobulus minutus, Dactylogyrus chalcalburni, D. vistulae, D. lenkorani, Gyrodactylus gobioninum, G. prostate, Paradiplozoon homoion, Allocreadium isoproum, Rhabdochona denudatai, Ligula intestinalis and Bothriocephalus acheilognathi, were isolated from the examined fishes. The results showed that host-parasite system in Alborz Dam is mainly influenced by the parasite’s fauna of Babol River. The only exception in this regard is the plerocercoids of L. intestinalis, one of the most common parasites in Alborz Dam and found in the abdominal cavity of all fish species. The prevalence rate and the mean intensity of parasitic infection in this reservoir are far more than those in Babol River. In addition this is the first report of Trichodina gracilis from the gills and skin of C. razii in Iran. Introduction The Babol River is one of main rivers in the Southern Caspian Sea basin in Mazandaran Province. The river originates from the northern slopes of the Alborz Mountains and flows toward the Caspian Sea after passing through the cities of Babol and Babolsar. The river flows in the mountainous part as a valley river with a notably slope. But as the river enters the Caspian coastal zone, the slope decreases rapidly and the river continues as a stream. The width of the Babol River in the Caspian coastal zone is about 40 kilometers (Ramin, 2001). The Alborz dam, with a height of 78 meters and volume of 150 million cubic meters, was built on the Babol River in 2010 to provide annual irrigation; it also is beneficial for flood protection and fisheries activities (Ghaffari et al., 2006). The Babol River provides a suitable habitat for *Corresponding author: Ali Taheri Mirghaed E-mail address: mirghaed@ut.ac.ir numerous species of migratory and non-migratory fishes belonging to the families of Petromyzonidae, Cobitidae, Nemacheilidae, Esocidae, Cyprinidae, Mugilidae and Gobiidae, but the largest number of fish species belongs to cyprinid family. Alburnoides tabarestanensis Mousavi-Sabet, AnvariFar & Azizi 2015; Capoeta razii Jouladeh-Roudbar, Eagderi, Ghanavi & Doadrio 2017 and Squalius turcicus De Filippi, 1865 are the most abundant fish species in the Babol River (Ramin, 2001; Esmaeili et al. 2017; Jouladeh-Roudbar et al., 2017) found in the Alborz Dam. Several parasitological studies have been conducted on the fishes inhabiting in rivers of the Southern Caspian Sea basin in Mazandaran Province. Molnár and Jalali (1992) have recorded the monogeneans Dactylogyrus lenkorani in the Tonekabon and Tajan rivers of the Caspian Sea basin 402 Barzegar et al./ Comparative study of fish parasites from the Babol River and Alborz Dam and D. pulcher in the Tajan, Tonekabon, and Ghasemlu rivers of the Caspian Sea basin as parasites of C. capoeta. Malek and Mobedi (2001) have reported Clinostomum complanatum from this species in the Shiroud River. Rohei Aminjan and Malek (2004) have found nine parasite species in fish from the Shiroud, namely trematodes C. complanatum, Diplostomum spathaceum, Postho- diplostomum cuticola and Allocreadium sp., the monogeneans D. pulcher, D. lenkorani and Gyro- dactylus mutabilitas, and nematodes Rhabdochona fortunatowi and Capillaria sp. Miar et al. (2008) have reported Myxobolus saidovi in the fish from the Valasht Lake and the Chalus River, Mazandaran. Maleki and Malek (2007) have reported infection of fish from the Shirud River in the Caspian Sea basin with the digeneans P. cuticola, D. spathaceum, C. complanatum, and Allocreadium sp. Jalali et al. (2005) have summarized the occurrence of Gyrodactylus species in Iran and recorded these species from fish in Sefid River. Barzegar and Jalali (2008) have reviewed crustacean parasites in Iran and found Ergasilus peregrinus, Ergasilus sp., Lamproglena compacta, Lernaea sp., Tracheliastes longicollis, and Tracheliastes polycolpus. Barzegar et al. (2008) have recorded the digenean eye parasite D. spathaceum from A. bipunctatus. The only research study on the fish parasites in the Babol River has been carried out by Hassanpour et al. (2004), who have recoded three metazoan parasite species, including Hepaticola petruschewskii from the intestine of A. bipunctatus (tabarestanensis), S. cephalus (turcicus) and C. gracilis (razii), and Capillaria sp., and Corynosoma villosum from the intestine of Rutilus kutum. So far, there is no report on fish parasites from the Alborz Dam. Communities of fauna and flora and the related parasites differ among the rivers and other water body; thus, different parasites are found in single hosts from two different ecosystems (Miar et al., 2008). These two water bodies, the Babol River and the Alborz Dam, belong to a single region, biogeographically, but with different ecosystems: one is a lotic, but the other is a lentic ecosystem. This study aimed to identify protozoan and metazoan parasites in some endemic fish species in the Babol River and the Alborz Dam in Mazandaran Province. It also aimed to compare parasite communities in the case of species diversity and the prevalence and intensity of parasite species among the infected members between the two different ecosystems. Materials and Methods Parasitological study: Field investigations were carried out in the Babol River and the Alborz Dam from June 2016 to March 2017. The fishes were caught by hook or electrofishing and immediately transported alive in oxygen-filled plastic bags to laboratory to be kept in aquaria. Identification of the fish was carried out according to Berg (1965), Coad (2017), and Keivany et al. (2017). Only fresh or immediately killed fish samples were examined for parasites. First, the fish were sedated using clove oil and then necropsied for parasitological analysis. The fish internal and external organs were examined. Methods used for collecting, fixing, staining, and mounting of the parasite specimens were carried out in accordance with Fernando et al. (1972), Gussev (1983), and Roberts (2001). The identification of parasites was carried following Gussev (1985), Lom and Dykova (1992), Yamaguti (1961), Woo (2000), and Jalali (1998). Data collection and management: To describe the parasite populations, some indices of infection, such as parasite prevalence and mean intensity were determined. Prevalence was defined as the number of individuals of a host species infected with one or more particular parasite species, divided by the total number of hosts examined for that parasite species (expressed as a percentage). The mean intensity was defined as the average intensity of a particular species of parasite among the infected members of a particular host species (Bush et al., 1997). Statistical analysis: To analyze of data, Excel and SPSS version 19 software were used. In this regard, Mann-Whitney U was used to compare the mean intensity of parasitic infection between the Babol River and the Alborz Dam. The values of P<0.05 403 Int. J. Aquat. Biol. (2017) 5(6): 401-407 were considered as significant. Results Approximately 546 fish specimens belonging to the cyprinid family, include A. tabarestanensis, C. razii, and S. turcicus, were examined (Table 1). A total of 13 protozoan and metazoan parasites were isolated from the examined fish of the Babol River and Alborz Dam. The species identified in the host fish include Ichthyophthirius multifiliis, Trichodina gracilis, Myxobulus minutus, Dactylogyrus chalcalburni, D. vistulae, D. lenkorani, Gyrodactylus gobioninum, G. prostate, Paradiplozoon homoion, Allocreadium isoproum, Rhabdochona denudatai, Ligula intestinalis and Bothriocephalus acheilognathi (Table 2). Monogenean parasites were the most abundant parasitic group in both river fish and dam fish (Fig. 1). The prevalence of the parasites among the examined fish in the Babol River and Alborz Dam are compared in Figure 2. Different fish species showed different prevalence; also prevalence of the Alborz Dam and Babol River is different. Mean intensity of the parasite species collected from the examined fish from the Babol River and Alborz Dam is also presented in Figure 3. There were significant difference in mean intensity of different parasites Table 1. Examined fish species from the Babol River and Alborz Dam in the southern part of the Caspian Sea basin, Iran. Examined fishes Length (cm) Weight (g) Number of specimens Number of infected fish A. tabarestanensis 6.9±0.2 7.20±0.6 114 94 C. razii 16.0±0.3 29.0±1.5 224 157.5 S. turcicus 12.3±0.4 14.8±0.6 208 187.5 Table 2. The parasite communities of some endemic fish species in the Babol River and Alborz Dam, in the southern Caspian Sea basin in Mazandaran Province. Parasite Host (S) Infected organs Ciliophora Ichthyophthirius multifiliis Fouquet, 1876 A. tabarestanensis Gills Alborz Dam, Babol River Trichodina gracilis Polyanski, 1995 C. razii Gills Babol River Myxozoa Myxobulus minutus Nemeczek, 1911 S. turcicus Gills Alborz Dam, Babol River Monogenea Dactylogyrus chalcalburni Dogiel & Bychowsky, 1934 A. tabarestanensis Gills Babol River Dactylogyrus vistulae Prost, 1957 S. turcicus Gills Alborz Dam, Babol River Dactylogyrus lenkorani Mikhailov, 1967 C. razii Gills Alborz Dam, Babol River Gyrodactylus gobioninum Gussev, 1955 C. razii Gills & skin Babol River Gyrodactylus prostae Ergens, 1963 C. razii A. tabarestanensis Gills & skin Babol River Paradiplozoon homoion Byckowsky et Nagibina, 1959 (Diporpa & adult stage) A. tabarestanensis S. turcicus Gills Alborz Dam, Babol River Digenea Allocreadium isoproum Looss, 1900 S. turcicus Intestine Babol River Cestoda Ligula intestinalis Linnaeus, 1758 A. tabarestanensis S. turcicus C. razii Abdominal cavity Alborz Dam Bothriocephalus opsariichthydis Yamaguti, 1934 A. tabarestanensis S. turcicus Intestine Alborz Dam, Babol River Nematode Rhabdochona denudata Dujardin, 1845 S. turcicus Intestine Babol River Figure 1. Frequency of different parasitic groups in the fish from the Babol River and Alborz Dam, in southern Caspian Sea basin in Mazandaran Province. http://researcharchive.calacademy.org/research/ichthyology/catalog/fishcatget.asp?genid=2867 http://researcharchive.calacademy.org/research/ichthyology/catalog/fishcatget.asp?genid=1720 http://researcharchive.calacademy.org/research/ichthyology/catalog/fishcatget.asp?spid=1989 http://researcharchive.calacademy.org/research/ichthyology/catalog/fishcatget.asp?genid=2867 http://researcharchive.calacademy.org/research/ichthyology/catalog/fishcatget.asp?genid=1720 404 Barzegar et al./ Comparative study of fish parasites from the Babol River and Alborz Dam among the fish species and between the Babol River and Alborz Dam. Overall, mean intensity of I. multifiliis, M. minutus, D. vistulae, D. lenkorani and L. intestinalis in the tested fish from the Alborz Dam was significantly higher than those of Babol River. However, T. gracilis, D. chalcalburni, G. gobioninum, G. prostate, A. isoproum and R. denudate in the tested fish from the Alborz Dam was significantly lower than those of Babol River. Discussion Only eight parasite species were isolated from the fishes in the Alborz Dam, of which seven species, including I. multifiliis, M. minutus, D. chalcalburni, Figure 2. Prevalence of different fish parasites in different fish species from the Babol River and the Alborz Dam in the southern Caspian Sea basin in Mazandaran Province. Figure 3. Mean intensity of fish parasite in different fish species from the Babol River and Alborz dam in the southern Caspian Sea basin in Mazandaran Province. Asterisks show significant difference between the Alborz Dam and Babol River. ** P<0.01; *** P<0.001; **** P<0.0001. 405 Int. J. Aquat. Biol. (2017) 5(6): 401-407 D. vistulae, D, lenkorani, P. homoion and B. opsariichthydis, are similar to those of Babol River. Miar et al. (2008) found similar results during their investigation on parasitic fauna of fish in the Valasht Lake and the Chaloos River. Based on their result, of 12 parasite species only three were isolated from the fish of the Valasht Lake. The Alborz Dam is a newly constructed and therefore the fish-parasite system in this reservoir is not completely formed and mainly influenced by the parasitic fauna of the River (Iziumova, 1987). The plerocercoids of L. intestinalis are of the most common parasites among examined fish which found only in the Alborz Dam. All the conditions for the incidence of Ligulose, including the high density of cyclops as the first intermediate host, the extensive presence of host fish species that feed on the cyclops as their preferred diet, and the fish-eating birds as definitive host are available in standing water of lakes and reservoir of dams (Jalali, 1998). In Iran, L. intestinalis has been reported from almost all dam reservoirs and lakes (Rouhani, 1998; Yousefi et al., 2005; Barzegar and Jalali, 2005; Jalali and Barzegar, 2006; Mohammadi Hefzabad and Ghare-Daghi, 2012). In the present study the prevalence rate of infection in C. capoeta and S. cephalus were 33% and 10% respectively, while the highest infection was observed in A. tabarestanensis (81%). An important aspect of the damage of l. intestinalis infestation is its effect on fish reproduction. The size of gonads even slightly infested fish is always smaller and participation in reproduction always ceases, which ultimately leads to a decrease in fish host populations (Parsa Khanghah et al., 2011). Besides direct losses caused by mortality, their presence may also reduce marketability of fish (Sac et al., 2016). This issue is important in commercially valuable fish and may cause great economic losses. Yousefi et al. (2005) recorded the occurrence of L. intestinalis in abdominal cavity of R. kutum with the prevalence rate of 100% in the Aras Dam. Bothriocephalus acheilognathi was isolated from A. tabarestanensis and S. turcicus from both Babol River and Alborz Dam. This parasite has spread throughout the world and grass carp and common carp are the hosts of this parasite (Xi et al., 2016). This parasite commonly infects native and endemic fish species in Iran. Bothriocephalus acheilognathi can cause massive fish kills in cultivated fish. However, it is a pathogen in the fish from natural environments (Salgado-Maldonado and Pineda-Lopez, 2003). This is the first report of B. acheilognathi in the intestine of A. tabarestanensis in Iran. The protozoan I. multifiliis was found on the skin of A. tabarestanensis in the Babol River and the Alborz Dam. Ichthyophthiriasis is recognized as one of the most pathogenic protozoan of fish resulting in significant economic losses in the affected fish (Jalali, 1998) and has been reported from many Iranian fish species (Pazooki et al., 2006; Jalali, 1998; Raissy et al., 2010). High infection rate with this parasite will have negative effect on fish population, a phenomenon that may occur in the Alborz Dam. Wurtsbaugh and Tapia (1988) reported a mass mortality of fish in the Lake Titicaca associated with an epizootic of the protozoan parasite, I. multifiliis. During the present study, adult stage of A. isoporum was found in the intestine of S. turcicus. Digeneans are heteroxenous, which means that they require more than one host to complete their life cycle and fish may also be infected by the metacercarial larval or adult stages (Paperna, 1964). Of course adult intestinal trematodes are normally considered unable to cause disease unless at high number (Paperna, 1964). Among the groups of parasites found in this study, monogeneans presented the highest number of species. Six species of monogeneans were found in the fish with D. lenkorani as the most abundant one. It is likely that fish protozoan and metazoan parasites, which are not dependent on an intermediate host, will be presented in their hosts. Their intensity may suddenly increase particularly when the hosts are under high stocking density (Lom and Hoffman, 1964). In the present study C. razii and A. tabarestanensis were introduced as a new host for P. homoion in Iran. Despite low number of the parasite species, the 406 Barzegar et al./ Comparative study of fish parasites from the Babol River and Alborz Dam prevalence and mean intensity parasite infection in the Alborz Dam is far more than Babol River. In most cases, the construction of a dam results in changes in fish biodiversity and stock abundance. Usually, the number of migrating fish species and fast flowing water species decline while stocks of pelagic species and species that prefer slow moving water such as A. tabarestanensis C. razii and S. turcicus (i.e. pre- adapted to lacustrine conditions) increase. The impounded waters have often been managed by introducing species better adapted to lacustrine environments to develop fishery activities. The high fish density in lakes and reservoirs along with other conditions including fairly constant environmental conditions, increase the chance of meeting the free living stages of parasites with their hosts and develop the outbreak of protozoan and metazoan parasites. In conclusion, both rapid and gradual changes of the environment can modify host immune responses, parasite communities and the specificity of their interactions so continuing such studies are powerful tools to understanding of how biotic and abiotic factors affect fish species and their parasite communities. References Barzegar M., Jalali B. (2005). Helminthes, Acanthocephala and Crustacean parasites of fishes in Vahdat Reservoir. Iranian Journal of Veterinary of Sciences, 3: 229-234. (In Persian) Barzegar M., Jalali B. (2008). Crustacean Parasites of Freshwater Fishes of Iran and Caspian Sea. A Review. Journal of Agricultural of Sciences and Technology, 11: 161-171. Barzegar M., Raissy M., Bozorgnia A., Jalali B. (2008). Parasites of the Eyes of Fresh and Brackish Water Fishes in Iran with Emphasis on Host Range and Geographical Distribution of Diplostomum spathaceum (metacercaria) Rudolphi, 1819. Iranian Journal of Veterinary Research, 9(3): 256-261. Berg L.S. (1965). Freshwater Fishes of USSR and Adjacent Countries. Vol. 3, Israel Program for Scientific Translations, Jerusalem, pp: 50-100. Bush A.O., Lafferty K.D., Lotz J.M., Shostak W. (1997). Parasitology meets ecology on its own terms: Margolis et al. Revisited. Journal of Parasitology, 83: 575-583. Coad B. (2017). Freshwater fishes of Iran. www.briancoad. com. Retrieved 3/19/2017. Esmaeili H.R., Mehraban H., Abbasi K., Keivany K., Coad B.W. (2017). Review and updated checklist of freshwater fishes of Iran: taxonomy, distribution and conservation status. Iranian Journal of Ichthyology 4(Suppl.1): 1-114. Fernando C.H., Furtado J.I., Gussev A.V., Hanek G., Kakonge S.A. (1972). Methods for the study of freshwater fish parasites, University of Waterloo. Biology series, Canada, 12, 76 p. Ghaffari G., Soleymani K., Saedi A. (2006). Investigating on the changes in morphology of the channel using geographic information system, Babolrood River, Iran. Journal of Geographical Research, 57: 61-71. Gussev A.V. (1983). The methods of collection and processing of fish parasitic monogenean materials, Nauka, Leningrad, USSR. 48 p. (In Russian) Gussev A.V. (1985). Parasitic metazoan. In: O.N, Bauer (Ed). Monogenea. Key to parasites of freshwater fish of USSR. Vol 2 . Nauka leningrad, USSR, 424 p. Hassanpour G., Ghasem-Zadeh F., Rahimian H. (2004). The first report of the introduction of three parasite species of Cyprinidae in Tajan and Babolrood River. Journal of Science, University of Tehran, 3(1): 13-35. Iziumova N.A. (1987). Parasitic fauna of reservoir fishes of the USSR and its evolution. Oxonian Press. 325 p. Jalali B. (1998). Parasites and parasitic diseases of fresh water fishes of Iran (In Persian). Fisheries Company of Iran, pp: 345-350. Jalali B., Barzegar M., (2006). Fish parasites in Zarivar Lake. Journal of Agricultural Sciences and Technology, 8: 47-58. Jalali B., Shamsi Sh., Barzegar M (2005). Occurrence of Gyrodactylus spp. (Monogenea: Gyrodactylidae) from Iranian freshwater fishes (In English). Iranian Journal of Fisheries Sciences, 4(2): 19-30. Jouladeh-Roudbar A., Eagderi S., Ghanavi, H.R., Doadrio I. (2017). A new species of the genus Capoeta Valenciennes, 1842 from the Caspian Sea basin in Iran (Teleostei, Cyprinidae). ZooKeys, 682: 137-155 Keivany Y., Nasri M., Abbasi K., Abdoli A. (2017). Atlas of inland water fishes of Iran. Iran. Department of Environment. 250 p. Lom J., Dykova I. (1992). Protozoan parasites of fishes. Elsevier Science Publisher. Amsterdam. Netherlands. 315 p. Lom J., Hoffman G.L. (1964). Distribution of some species 407 Int. J. Aquat. Biol. (2017) 5(6): 401-407 of Trichodinids (Ciliata: Peritricha) parasitic on fishes. Journal of Parasitology, 50(1): 30-35. Malek M., Mobedi. I. (2001). Occurrence of Clinostomum complanatum (Rudolphi, 1819) (Digenea: Clinostomatidae) in Capoeta capoeta gracilis (Osteichthys: Cyprinidae) from Shiroud River, Iran. Iranian Journal of Public Health, 30(3): 95-98. Maleki L., Malek M. (2007). Digenean parasites of Capoeta capoeta and Chalcalburnus chalcoides (Osteichthyes: Cyprinidae) in Shiroud River. Journal of Science of University of Tehran, 32(4): 369-373. Miar A., Bozorgnia A., Pazooki J., Barzegar M., Masoumian M. and Jalali B. (2008). Identification of parasitic fauna of fishes in Valasht Lake and Chalus River. Iranian Scientific Fisheries Journal, 17(1): 133- 138. (In Persian) Mohammadi Hefzabad M., Ghare-Daghi Y. (2012). Report of several cases infection of Chalcalburnus chalcoides by Ligula intestinalis in Sangar Dam, Guilan Province. Journal of Veterinary Clinic Pathology (Veterinary Medicine, Tabriz), 2(6): 1579-1582. Molnár K., Jalali B. (1992). Further monogeneans from Iranian freshwater fishes. Acta Veterinaria Hungarica, 40(1-2): 55-61. Paperna I. (1964). Host reaction to infestation of carp with D. vistulae Nybelin, 1924 (monogenean). Bamidgeh, 16(4): 1249-141. Parsa Khanghah A., Mojazi Amiri B., Sharifpour I., Jalali Jafari B., Motalebi A.A. (2011). Gonads tissue changes of Chalcalburnus mossulensis (Heckel, 1843) infected by Ligula intestinalis (cestoda). Iranian Journal of Fisheries Sciences, 10(1): 85-94. Pazooki J., Masoumian M., Jafari N. (2006). Tehran: Iranian Fisheries Research Organization. Check-list of Iranian fish parasites. (In Persian) Raissy M., Ansari M., Lashkari A., Jalali B. (2010). Occurrence of parasites in selected fish species in Gandoman Lagoon. Iranian Journal of Fisheries Sciences, 9(3): 17-21. Ramin M. (2001). Identification of the fishes of Babolrood River, Mazandaran, Iran. Iranian Journal of Fisheries Sciences, 6 (3): 59-72. Rohei Aminjan A., Malek M. (2004). Ecology of helminthes parasites of Capoeta capoeta gracilis from Shiroud River, Iran. Iranian Journal of Fisheries Sciences, 13(2): 73-82. (In Persian) Rouhani M. (1998). An investigation on parasitic infections and diseases of the fishes in the Sistan Province. The Second Meeting of Veterinarians in Clinical Sciences of Iran. 30-28 November, Tehran. Iran. Sac G., Serezli E.E., Okgerman H. (2016). The occurrence of Ligula intestinalis in its fish host Rutilus rutilus (L.) and the effects of parasite on the fish growth (Buyukcekmece Reservoir, Turkey). Journal of Engineering and Fisheries Research, 2(3): 142-150. Salgado-Maldonado G., Pineda-Lopez R.F. (2003). The Asian fish tapeworm Bothriocephalus acheilognathi: a potential threat to native freshwater fish species in Mexico Guillermo. Biological Invasions, 5: 261-268. Woo P. (1999). Protozoan and metazoan infections, Vol. 1, CABI Pub. London, UK. 775 p. Wurtsbaugh W.A., Tapia R.A. (1988). Mass mortality in Lake Titicaca (Peru-Bolivia) associated with the protozoan parasite Ichthyophthirius multifiliis. Transaction of American Fisheries Society, 117(1): 213-217. Xi B., Li W., Wang W., Nie P., Xie J. (2016). Cryptic genetic diversity and host specificity of Bothriocephalus acheilognathi Yamaguti, 1934 (Eucestoda: Bothriocephalidea). Zoological Systematics, 41(2): 140-148. Yamaguti S. (1961). Systema helminthum, Monogenea and Aspidocotylea, Interscience Publishers. 699 p. Yousefi M.R., Sefid-gar A.A., Maliji G., Mousavi J., Asna- Ashari M.Y. (2005). Report of several cases infection of Rutilus rutilus by Ligula intestinalis in Aras Dam. Journal of Medical Sciences, 2(26): 80-83. Int. J. Aquat. Biol. (2017) 5(6): 401-407 E-ISSN: 2322-5270; P-ISSN: 2383-0956 Journal homepage: www.ij-aquaticbiology.com © 2017 Iranian Society of Ichthyology چکیده فارسی ریاید آبریز هضحو شرقی جنوب در البرز سد و بابلرود رودخانه بومی ماهیان برخی انگلی جمعیت ایمقایسه بررسی مازندران استان در خزر 2بزرگنیا عباس ،1هوالسو رحمتی هومن ،1موسوی زاده ابراهیم حسینعلی ،1برزگر مریم، 1*میرقائد طاهری علی ، ایران.تهران تهران، دانشگاه دامپزشکی دانشکده آبزیان هایبیماری و بهداشت گروه1 .ایران قائمشهر، قائمشهر، واحد اسالمی آزاد دانشگاه طبیعی، منابع دانشکده آبزیان و شیالت گروه2 چکیده: منظوربه رودخانه این امتداد در البرز سد و بوده مازندران استان در خزر دریای آبریز هضحو شرقیجنوب هایرودخانه ترینآب پر از یکی بابلرود میزان تشریح و شناسایی تحقیق این اصلی هدف. است شده احداث سیل خطر برابر در مناطق حفاظت و آبیاری جهت نیاز مورد آب آوردن فراهم تحقیقات. است مذکور ماهیان هایانگل مقایسه و البرز سد و بابلرود بومی ماهیان برخی پریاخته و یاخته تک هایانگل به آلودگی شدت و شیوع ورازی ماهیسیاه ،طبرستانی خیاطهماهی شامل کپورماهیان خانواده به متعلق ماهی نمونه 546 تقریباً و شده انجام 1396 سال طی در میدانی ،Ichthyophthirius multifili، Trichodina gracilis شامل انگل گونه 13 مجموع در. گرفتند قرار شناسیانگل بررسی مورد ایرودخانه سفید Myxobulus minutus، Dactylogyrus chalcalburni، D. vistulae، D. lenkorani، Gyrodactylus gobioninum، G. prostate، Paradiplozoon homoion، Allocreadium isoproum، Rhabdochona denudatai، Ligula intestinalis و Bothriocephalus opsariichthydis أثرمت اساساً البرز سد دریاچه در میزبان-انگل سیستم که دهدمی نشان نتایج. شدند جدا بررسی مورد ماهیان مختلف هایاندام از دریاچه در انگلی هایونهگ ترینفراوان از یکی که است اینتستینالیس لیگوال پلروسرکوئید زمینه این در استثناء تنها .است بابلرود رودخانه انگلی فون از مختلف هایگونه به آلودگی شدت و شیوع درصد که است حالی در این. شد یافت بررسی مورد ماهیان تمامی شکمی محوطه در و بوده البرز سد پوست و بششآ از گراسیلیس تریکودینا حضور گزارش اولین حاضر بررسی این، بر عالوه. است بابلرود از بیشتر مراتب به البرز سد دریاچه در انگلی .است ایران در ماهی سیاه .البرز سد بابلرود، ماهی، انگل ،دریای خزر :کلمات کلیدی