Proceeding of Veterinary and Animal Science Days 2018, 6th- 8th June, Milan, Italy HAF © 2013 Vol. V, No. 1s ISSN: 2283-3927 l To date, knowledge about leukemias in feline patients is poor. Classification of leukemias is mostly based on morphological, immunological and genetic aspects in dogs and humans (Harvey, 2012). However, in cat poor literature about classification of leukemias via flow cytometric (FC) immunophenotyping is available and just a few publications of case reports are present (Gelain et al., 2006; Sharifi et al., 2007; Shirani et al., 2011). We retrospectively selected 44 cases of leukemias from our FC service database from 2009 to 2017 with the aim to describe the major immunological features and define the prevalence of different subtypes. Blood and/or bone marrow submitted for FC analysis with a prevalent hematological presentation were selected. Cases with an evident lymph node enlargement or with clinical aspects suggesting a solid lesion (lymphoma) were excluded. Cases were classified depending on antigen expression and hematologic features in five groups: chronic lymphocytic leukemias (CLL), acute lymphoblastic leukemias (ALL), acute myelogenous leukemias (AML), acute undifferentiated leukemias (AUL) and undetermined ALL vs lymphoma stage V. 26 cases (59%) were classified as acute leukemias whereas 11 cases (25%) were classified as chronic leukemias; 7 cases (16%) were classified as undetermined. Among acute leukemias 10 (38.5%) were AML, 10 (38.5%) AUL and 6 (23%) ALL. All ALL were of T cell origin (CD3+ or CD5+). Among chronic leukemias, 10 (91%) were of T cell origin and among these, 80% expressed CD4 (T-helper lymphocytes), while 20% were double negative (CD4- CD8-). These results confirmed that T cell leukemias are more frequent than B ones in the cat with a prevalent T-helper phenotypes as previously described (Helfand and Kisseberth, 2010). AMLs were highly represented, but the lack of an adequate panel of specific antibodies for myeloid lineage rendered a high number of AUL in our caseload. Similarly, the lack of an anti-feline CD34 antibody did not permit differential diagnosis of acute leukemia vs lymphoma with blood involvement in a remarkable percentage of cases without an evident nodal enlargement and without an extreme leukocytosis. Keywords Feline, Flow cytometry, Leukemia, Classification. CORRESPONDING AUTHOR Serena Bernardi serena.bernardi@unimi.it JOURNAL HOME PAGE riviste.unimi.it/index.php/haf Immunophenotypical and cytomorphological examination of feline peripheral blood in patients with suspect leukemia. S. Bernardi1*, V. Martini1, S. Costanzo1, M. Cozzi1, S. Comazzi1 1 Department of Veterinary Medicine, Università degli Studi di Milano, Via dell’Università 6, 26900 Lodi, Italy. http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en Proceeding of Veterinary and Animal Science Days 2018, 6th- 8th June, Milan, Italy 58 HAF © 2013 Vol. V, No. 1s ISSN: 2283-3927 References Gelain M.E., Antoniazzi E., Bertazzolo W., Zaccolo M., Comazzi S., 2006. Chronic eosinophilic leukemia in a cat: cytochemical and immunophenotypical features. Vet Clin Path. Dec,35 (4): 454-9. Harvey J.W., 2012. Veterinary hematology: a diagnostic guide and color atlas. Saunders, Elsevier inc. Helfand, C.S and Kisseberth, W.C., 2010. General Features of leukemia and lymphoma. Schalm’s Veterinary hematology. Blackwell publishing Ltd. p 455-465. Sharifi H., Nassiri S.M., Esmaelli H., Khoshnega J., 2007. Eosinophilic leukemia in a cat. J Feline Med Surg. Dec,9(6): 514-7. Shirani D., Nassiri S.M., Aldavood S.J., Sheddig H.S., Fathi E., 2011. Acute erythroid leukemia with multilineage dysplasia in a cat. Can Vet J. Apr,52(4): 389-93. Figure 1: Peripheral blood: evaluation of morphology of white blood cells in a blood sample with suspect acute leukemia. Large mononuclear cells are present, with modest basophilic cytoplasm, rounded nuclei with a single large nucleolus and dispersed chromatin. FC analysis revealed a CD14 positivity and negativity to common lymphocyte markers: the final diagnosis was AML. http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en