Proceeding of Veterinary and Animal Science Days 2017, 6th- 8th June, Milan, Italy HAF © 2013 Vol. IV, No. 1s ISSN: 2283-3927 l Keywords Eels, Perfluoroalkyl substances, LC-HRMS CORRESPONDING AUTHOR Maria Nobile maria.nobile1@unimi.it JOURNAL HOME PAGE riviste.unimi.it/index.php/haf Preliminary study about the detection of perfluoroalkyl substances in eel samples of Lake Garda by liquid chromatography tandem mass high resolution mass spectrometry (LC-HRMS). Maria Nobile1*, Sara Panseri1, Francesco Arioli1, Luca M. Chiesa1 1 University of Milan, Department of Health, Animal Science and Food Safety, Italy Abstract Perfluoroalkyl substances (PFASs) are a large class of fluorinated aliphatic chemical of anthropogenic origin with high chemical stability even at high temperatures and in presence of alkalis, strong acids or oxidizing agents (Lau et al. 2004). All these characteristics make them no biodegradable and very persistent in the environment, associated with adverse health risks (Eriksen et al. 2010). Food, especially fish and other seafood, is considered the main source of exposure to PFASs (EFSA, 2012). In this preliminary study we developed and validated a sensitive, selective and specific method by LC- HRMS Orbitrap to monitor the presence of 16 PFASs in eel (Anguilla Anguilla) samples. The clean-up of the lyophilized samples consisted of a previous extraction step with acetonitrile to precipitate also proteins, followed by a purification step through Oasis® WAX SPE (Weak Anionic Exchange Solid Phase Extraction) cartridges. The method applied to 45 farmed eel samples from Lake Garda showed the presence of several PFASs, up to 10 in the same eel, in the order of ng/g (Fig.1). The results provided a representative situation of the PFASs contamination level of the lake, lower than those of others European countries (Hoff et al. 2005, Kwadijk et al. 2010). http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en Proceeding of Veterinary and Animal Science Days 2017, 6th- 8th June, Milan, Italy HAF © 2013 Vol. IV, No. 1s ISSN: 2283-3927 Fig.1: Average concentrations (ng/g) of the PFASs detected in the 45 eel samples of Lake Garda. PFBA: perfluoro-n-butanoic acid; PFPeA: perfluoro-n-pentanoic acid; PFHpA: perfluoro-n-heptanoic acid; PFOA: perfluoro-n-octanoic acid; PFNA: perfluoro-n-nonanoic acid; PFOS: sodium perfluoro-1-octanesulfonate; PFDA: perfluoro-n-decanoic acid; PFUdA: perfluoro-n- undecanoic acid; PFDoA: perfluoro-n-dodecanoic acid; PFTrDA: perfluoro-n-tridecanoic acid; PFTeDA: perfluoro-n- tetradecanoic acid. References EFSA, 2012. Perfluoroalkylated substances in food: occurrence and dietary exposure. EFSA Journal. 10 (6):2743, 1-55. Eriksen, K.T., Raaschou-Nielsenb, O., Sørensen, M., Roursgaard, M., Loft, S., Møller, P., 2010. Genotoxic potential of the perfluorinated chemicals PFOA, PFOS, PFBS, PFNA and PFHxA in human HepG2 cells. Mutation Research. 700, 39–43. Hoff, P.T, Van Campenhout, K., Van de Vijver, K., Covaci, A., Bervoets, L., Moens, L., Huyskens, G., Goemans, G., Belpaire, C., Blust, R., De Coen, W., 2005. Perfluorooctane sulfonic acid and organohalogen pollutants in liver of three freshwater fish species in Flanders (Belgium): relationships with biochemical and organismal effects. Environmental Pollution. 137, 324–333. Kwadijk, C.J.A.F., Korytar, P., Koelmans, A.A., 2010. Distribution of perfluorinated compounds in aquatic systems in the Netherlands. Environmental Science & Technology. 44, 3746–3751. Lau, C., Butenhoff, J.L., Rogers, J.M., 2004. The developmental toxicity of perfluoroalkyl acids and their derivatives. Toxicology and Applied Pharmacology 198(2), 231–241. http://creativecommons.org/licenses/by-nc-nd/3.0/deed.en