Iraqi J Pharm Sci, Vol.29(1) 2020 Mirtazapine nanoparticles DOI: https://doi.org/10.31351/vol29iss1pp62-75 62 Preparation, in vitro and ex-vivo Evaluation of Mirtazapine Nanosuspension and Nanoparticles Incorporated in Orodispersible Tablets Hiba E. Hamed*,1 and Ahmed A. Hussein* *Department of Pharmaceutics, College of Pharmacy, University of Baghdad, Baghdad, Iraq, Abstract The objective of the present investigation was to enhance the solubility of practically insoluble mirtazapine by preparing nanosuspension, prepared by using solvent anti solvent technology. Mirtazapine is practically insoluble in water which act as antidepressant .It was prepared as nano particles in order to improve its solubility and dissolution rate. Twenty formulas were prepared and different stabilizing agents were used with different concentrations such as poly vinyl pyrrolidone (PVPK-90), poly vinyl alcohol (PVA), poloxamer 188 and poloxamer 407. The ratios of drug to stabilizers used to prepare the nanoparticles were 1: 1 and 1:2. The prepared nanoparticles were evaluated for particle size, entrapment efficiency, dissolution study, Fourier transform infrared spectroscopy, differential scanning calorimetry, and atomic force microscopy. The percentage of drug entrapment efficiency of F1-F20 was ranged from 78.2% ± 1 to 95.9 % ± 1. The release rate and extent of mirtazapine nanoparticles were inversely proportional to the particle size of the drug i.e. it decreased when particle size increase. It is concluded that the nanoprecipitation have potential to formulate homogenous nanosuspensions with uniform-sized stable nanoparticles of mirtazapine. The prepared nanosuspension showed enhanced dissolution which may lead to enhanced solubility of mirtazapine. Keywords: Mirtazapine, Nanoparticles, Particle Size, Poloxamer. النانويةنانوي و الجسيمات الميرتازابين المعلق لتحضير وتقييم في المختبر وخارج الجسم الحي للتشتت ةفي اقراص قابل مدمجة *حسين احمد عباس و 1*،هبه عزت حامد .العراق ،بغداد ،بغداد ةجامع الصيدلة، كلية ،الصيدالنيات *فرع الخالصة للذوبان عمليا من خالل اعداد تعليق نانوي , تم اعداده القابلةهو تعزيز قابليه الذوبان لعقارالميرتازابين غير الدراسةان الهدف من هذه ميرتازابين .الشديداالكتئاب يستخدم لعالجء لاللمذيبات. ميرتازابين هو دواء غير ذائب بالماء وهو دوا المضادةباستخدام باستخدام تكنلوجيا المذيبات للذوبان ومعدل االمتصاص. القابليةبغيه تحسين نانويةاعد كجسيمات , وبولي الفنيل PVPمثل مثل الفاينيل بايرولدون المتعدد مختلفةبتراكيز مختلفة استخدمتتم اعداد عشرين صيغه باستخدام بوليمرات استقرار .1:2, 1:1في اعداد الجسيمات النانويه هي المستخدمةكانت نسب الدواء الى المثبتات . و407و بولوكسامير 188, بولوكسامير PVAالكحول التوافق دراسةوكذلك الدوائي،الشكل البياني للتحرر ودراسة الدواء،من حيث الحجم الحبيبي للجسيمات وكفاءه انحباس نانويةوقيمت جسيمات االولى الصيغةمن الدوائيةانحباس الدواء للصيغ لكفاءة المئوية النسبة. الذرية القوةي( ومجهر وقياس المسح التفاضل الحمراء،تحت األشعة)مطيافيه السطحية المساحة لزيادة النانويةاخرى يزداد تحرر الدواء كلما صغر حجم الجسيمات ناحية. من %95,9الى %78,2من الصيغة عشرين هيالى ان الجسيمات النانوية لديها القدرة على صياغة تعليق متجانس مع جسيمات نانوية مستقرة موحدة الحجم من الميرتازابين. ويمكن استنتاجللجسيم. .االنحالل يؤدي إلى تعزيز الذوبان من الميرتازابين ان تعزيزوأظهرت .بولوكسامير ن،الحبيبيالحجم ،النانوية الجسيمات ،ميرتازابين: المفتاحيةالكلمات Introduction Solubility is of the most important parameter to achieve the desired concentration of drug in systemic circulation for pharmacological response to be shown. Poorly water soluble drugs often require high doses in order to reach therapeutic plasma concentrations after oral administration. Low water solubility is the major problem encountered with formulation development of new chemical entities(1). Several formulation techniques exist for the manufacturing of nanosuspension, precipitation has been applied to prepare submicron particles, especially for the poorly soluble drugs. Rapid addition of a drug solution to the anti-solvent leads to sudden super saturation of drug and formation of ultrafine crystalline or amorphous drug solids(2). Mirtazapine is an antidepressant drug used for the treatment of moderate to severe depression, molecular formula: C17H19N3 (3). The drug has bioavailability of 50 % due to first-pass metabolism, high protein binding (80 %) and very high half-life (20 – 40 h)( 4). The aim of this study is to formulate and evaluate mirtazapine nanoparticles using solvent anti solvent method. Corresponding author E-mail: hibaa.19855@gmail.com Received:11 /6/2019 Accepted: 29/ 9/2019 Iraqi Journal of Pharmaceutical Science https://doi.org/10.31351/vol29iss1pp62-75 mailto:hibaa.19855@gmail.com Iraqi J Pharm Sci, Vol.29(1) 2020 Mirtazapine nanoparticles 63 Materials and Method Materials Mirtazapine powder was purchased from (Hyper-chem, China), PVP K-90(Hangzhou Sunflower ,China),PVA(JP&SB Converting Services, Spain), poloxamer 188 and poloxamer 407(HIMEDIA (Mumbai, India), methanol (Scharlau Chemie, S.A. Spain). All other chemicals were of analytical grade. Method Preparation of mirtazapine nanosuspension Nanosuspensions of mirtazapine were prepared by the solvent evaporation technique, which is also termed as anti-solvent precipitation method. Mirtazapine powder was dissolved in methanol (4 ml) at room temperature. This was poured into 20 ml of water containing different types of stabilizer (alone and in combination) maintained at 500C and subsequently stirred at agitation speed of 500 revolution per minute (rpm) on magnetic stirrer for 60 min.to allow the volatile solvent to evaporated (5). The resultant organic solution of drug (organic phase) was added drop by drop by means of a plastic syringe positioned with the needle directly into aqueous solution of stabilizer. The ratios of drug to stabilizer used to prepare the nanosuspension were 1:1 and 1:2, as shown in table (1,2). Table1. Composition of mirtazapine nanosuspension using different stabilizers at drug: stabilizer ratio 1:1. Formula Mirtazapine (mg) Poloxamer 188 (mg) Poloxamer407 (mg) PVP-k90 (mg) PVA (mg) Methanol (ml) Water (ml) F1 15 15 4 20 F2 15 15 4 20 F3 15 15 4 20 F4 15 15 4 20 F5 15 7.5 7.5 4 20 F6 15 7.5 7.5 4 20 F7 15 7.5 7.5 4 20 F8 15 7.5 7.5 4 20 F9 15 7.5 7.5 4 20 F10 15 7.5 7.5 4 20 Table2. Composition of mirtazapine nanosuspension using different stabilizers at drug: stabilizer ratio 1:2 Water (ml) Methanol (ml) PVA (mg) PVPk-90 (mg) Poloxamer407 (mg) Poloxamer 188 (mg) Mirtazapine (mg) Formula 20 4 30 15 F11 20 4 30 15 F12 20 4 30 15 F13 20 4 30 15 F14 20 4 15 15 15 F15 20 4 15 15 15 F16 20 4 15 15 15 F17 20 4 15 15 15 F18 20 4 15 15 15 F19 20 4 15 15 15 F20 Iraqi J Pharm Sci, Vol.29(1) 2020 Mirtazapine nanoparticles 64 Evaluation of the prepared nanosuspension Particle size and size distribution Particle size determination was done by using Angstrom Advanced Inc. ABT-9000 USA particle size analyzer which is a dynamic light scattering works by measuring the intensity of light scattered by the molecules in the sample as a function of time, at scattering angle 90° and a constant temperature of 25 °C. The polydispersity index (PDI) which is a measure of the width of the size distribution of each formula of mirtazapine nanosuspension was measure of the distribution of particle size of nanoparticles obtained from a particle analyzer, PDI is an index of spread or variation or width within the particle size distribution. Also, the analyzer determines the specific surface area of each sample(6) . Determination of drug entrapment efficiency (EE) of nanosuspension The freshly prepared nanosuspension of drug: stabilizer ratio 1:1, and 1:2 was centrifuged at 20,000 rpm for 20 minutes using ultracentrifuge. The amount of unincorporated drug was measured by taking the absorbance of the appropriately diluted 25 ml with water at 290 nm using UV-visible spectrophotometer. It was calculated by subtracting the amount of free drug in the supernatant from the initial amount of drug taken. For each formulation the experiment was repeated in triplicate and the average was calculated (7). In vitro dissolution profile of nanosuspension The in vitro dissolution study was performed using USP dissolution test apparatus-II (paddle assembly). The dissolution was performed using mirtazapine nanosuspension in 900 ml of 0.1N HCL (pH 1.2) maintained at 37 ± 0.5°C , 50 rpm and samples (5ml) were withdrawn at regular intervals of 5 minutes for 120 minutes and replaced with fresh dissolution medium. Samples were filtered through filter paper and assayed spectrophotometrically on UV-Visible spectrophotometer at 315 nm wave length (8). Freeze drying of nanosuspension In order to make nanoparticles in dried- powder state from the nanosuspensions, water- removal was conducted through freeze-drying, so that each formula was lyophilized using vacuum freeze dryer at a controlled temperature of (- 45) °C and the pump operating at a pressure of 2.5 × 10 pascal over a period of 48–72 hour. The yielded powders were used for further studies and also it is used to prepare the tablets (9). Formation of mirtazapine nanoparticles tablet Mirtazapine formulated in to orodispersible tablets by direct compression method containing drug equivalent to 15mg mirtazapine. All ingredients were properly mixed to gather then compressed in to tablets prepared by after freeze drying of formula (F15 ) that gave the best in vitro dissolution profile in minute in comparison with other nanoparticle formulas and pure drug, show as in table (3) (10). The orodispersible tablets were prepared using Avicel PH102 (MCC), crospovidone and, magnesium stearate as a diluent, disintegrente ,and lubricant at different concentration and tested to obtain the optimum formula that show the accepted hardness and the best in vitro dissolution profile(11). Table 3. Composition of mirtazapine tablets Quantity per tablet (mg) Materials F 15 b F 15 a 45 45 Lyophilized Powder 92 82 Avicel PH 102 (MCC) 10 20 Crospovidone 3 3 Magnesium Stearate 150 150 Tablet Weight (mg) Precompression studies of the prepared nanoparticle powder The flowability of a powder is of critical importance in the production of pharmaceutical dosage forms in order to get a uniform feed as well as reproducible filling of tablet dies otherwise high dose variations will occur. The powder flowability of prepared mirtazapine tablets were characterized by angle of repose, Hausner's ratio and Carr’s index (12). Evaluation of Mirtazapine Orodispersible Tablets Tablets were evaluated for hardness test, friability test, content uniformity test and weight variation tests (13), and dissolution study. In vitro dissolution profile of mirtazapine tablets An in vitro dissolution test was conducted in a dissolution apparatus according to the USP paddle method. The temperature was maintained at 37 ± 0.5°C, and the stirring rate was at 50 rpm. The commercial mirtazapine tablet accurately weighed bulk drug and were dispersed in 900 ml of dissolution medium (0.1 N HCl). 5 ml samples were drawn, and the same volume of fresh dissolution medium was added at 5, 10, 15, 20, 30, 45, 60, 70, 90, 105, 120 minutes.. Then, the samples were filtered through a 0.1-μm syringe filter immediately before dilution, when necessary. Drug content was determined with a UV spectrophotometer at 315 nm for 0.1 N HCl (pH 1.2) (14). Iraqi J Pharm Sci, Vol.29(1) 2020 Mirtazapine nanoparticles 65 Fourier transform infrared spectroscopy (FTIR) The (FTIR) spectra were recorded for pure drug and optimized formulation using KBr pellet technique.The pellets were prepared using KBr hydraulic press under hydraulic pressure of 150 kg/cm2 . The spectra were scanned over 3600-400 cm−1 at ambient temperature with a resolution of 4 cm−1, using FT-IR 2500 apparatus (15). Differential scanning calorimetry (DSC) DSC investigations were performed using DSC apparatus model DSC-6. Samples of about 5 mg of pure drug powder and selected formula are placed in an aluminum pan and the experiment was carried out under nitrogen atmosphere at a flow rate of 40 mL/min and scanning rate of 10°C/min in the range of 15-300°C(16). High performance liquid chromatographic (HPLC) RP-HPLC system was used for this study and the specifications are given below. A Waters HPLC equipped with SPA- 20A detector, an isocratic chromatographic separation technique was conducted utilizing Symmetry® ODS-C18 (250 × 4.6mm; 5μm) column and Breeze software. Chromatographic conditions: Mobile phase: HPLC grade of Methanol: 0.1M ammonium formate solution in a ratio of 77:23 percent (v/v) was filtered through (0.45μm) Millipore filter. Flow rate: it was maintained at 1.0 ml/min of the mobile phase. Detection was carried out by UV- detector; at a wavelength of 315 nm and the running time was 10 min. One hundred milligrams of mirtazapine was accurately weighed and transferred to a 100 ml volumetric flask. It was dissolved in 50 ml HPLC grade methanol and sonication for about 10 minutes and then made up to the volume with HPLC grade methanol. From this stock solution (1mg/ml) eight serial dilutions (1.66, 2.5, 5, 10, 20, 30, 40, and 50 μg/ml) were prepared. Twenty microliters of each dilution were injected into the column and the corresponding chromatograms were obtained (17). Atomic force microscopy (AFM) The AFM is capable of scanning the surfaces in controlled environmental conditions and is complementary to SEM imaging. The size and surface morphology of mirtazapine nanoparticle were confirmed by atomic force microscopy of the formula. Samples were determined in tapping mode, exerting pyramidal cantilevers with Pt probes. All results were recorded under ambient laboratory condition and scanning frequency of 2Hz. Resonance frequency was 79.491 kHz and a constant force in the range 2.5-10Nm-1, driving amplitude 334.6mv. silicon chip was newly operated by peeling off its upper layer to Form the sample. Particle size, 3D-dimension graph and histogram of particle size distribution were obtained(18). Statistical Analysis The results of the experiments were given as a mean of triplicate samples ± standard deviation and were analyzed according to the paired T test and one way analysis of variance (Single Factor ANOVA) at the level of (P < 0.05). Results and Discussion Evaluation of nanosuspension Particle size analysis The particle size of F1-F4 at drug : stabilizer ratio 1:1 was ranged from 429 - 691 nm measured by particle size analyzer (as shown in table 4) while for F11-F14 at drug : stabilizer ratio 1:2 the particle size ranged from 379 - 572 nm as in table (4) using poloxamer 188 , poloxamer 407 , PVP- K90 and PVA as primary stabilizers. PVP K-90 , poloxamer and PVA are stabilizers for nanosuspension. Vinyl groups of PVA (Polyvinyl alcohol), due to their hydrophobic nature tend to adsorb onto the hydrophobic part of mirtazapine nanoparticles while –OH extend themselves outside into the aqueous environment and thus providing stabilization to the nanoparticles and preventing agglomeration. –OH bonds of PVA makes hydrogen bonding with water molecules and thus viscosity of it increases (19). Polydispersity index is a parameter used to define the particle size distribution obtained from the particle size analyzer. Polydispersity index gives degree of particle size distribution at range from 0.021 to 0.420 depending on formulation variables. The formula F10 showed lowest PDI (0.029) at drug : stabilizer ratio 1:1 and 0.114 at drug : stabilizer ratio 1:2, as seen in table (4 ) ; that indicate good uniformity of nanoparticle size. Uniformity of particle size is determined by polydispersity index values in which the low value means the best uniformity(20). The range of PDI values (0-0.05) means (monodisperse system), 0.05-0.08 (nearly monodisperse), 0.08-0.7 (mid-range polydispersity), and >0.7 (very polydispersity). From the obtained results, one can conclude that the poloxamer 188 and poloxamer 407 are suitable as a primary stabilizer for nanoparticles because of poor adsorption and affinity of poloxamer to the drug molecules. Effect of polymer concentration on the size of Mirtazapine nanoparticles The effect of the stabilizer concentration on the particle size was investigated by depending on two ratios 1:1 of drug : stabilizer in the preparation of F1-F10 and 1:2 of drug : stabilizer in the preparation of F11-F20. Not only the type of stabilizer affects the particle size, but also the concentration of the stabilizers used. Stabilizer concentration also influences on the adsorption affinity of non-ionic stabilizers to particle surface. Iraqi J Pharm Sci, Vol.29(1) 2020 Mirtazapine nanoparticles 66 In general, as the concentration of stabilizer increase the particle size decrease at fixed drug concentration, the concentration of stabilizer may give negative effect (decrease particle size) or positive effect of on particle size (increase particle size). It can also influence on the adsorption affinity of non-ionic stabilizers to particle surface. In general, as the concentration of stabilizer increases the particle size decreases at fixed drug concentration.(21). It was observed that with an increase in surfactant concentration in the nanosuspension from the particle size of the nanosuspension decreases. This was due to the decrease in relative viscosity, which led to decrease in particle size. It means that hydrodynamic diameter of particle decreased with increase in the concentration of the surfactant.The concentration of surfactant affected on particle size because too little concentration of stabilizer induces agglomeration or aggregation of particles (22). As shown in tables (4,5) the size range of particles is decrease in the sequence of F1 (429nm) > F11 (383 nm), F2 (444nm) > F12 (401nm) that correspond to 1:1, 1:2 of drug: stabilizer (poloxamer 188, poloxamer 407) ratio, respectively. These results indicated that mean size of particles showed a regular decrease with increasing the concentration of poloxamer. These effects due to a process of a primary covering of the newer surfaces competing with the aggregation of the uncovered surfaces. Hence, an elevation in ratio of surfactant in the primary dispersion results in rapid enclosing of the newly formed particle surfaces. There was an optimum concentration of surfactant, above which the increase in concentration did not result in a decrease in particle size due to saturation point; these results are in agreement when poloxamer was used as stabilizer at different ratio (23). Poloxamer is a block co-polymer, can act as a surfactant, is responsible for the hydrophobic association with the molecules of drug. The inhibition of the crystal growth is mainly related to the hydrophobic part (polypropylene oxide group PPO) in the pluronic polymer, while the second chain which is (the hydrophilic oxide) (PEO) can provide steric hindrance against particles aggregation(24). The size range of particles is also decreased in the sequence F3 (460) > F13 (379nm), F4 (691nm) > F14(572nm) that correspond to 1:1, 1:2 of drug: stabilizer PVA, PVP k-90 ratio, respectively. On the other hand, the adsorption of surfactant makes the particles less hydrophobic and thereby reduces the hydrophobic forces of attractions (van der Waals interactions) and that reduced particle growth and aggregation(25). Effect of combination of two polymers on the size of mirtazapine nanoparticles The particle size of ( F5-F10) of drug : stabilizer ratio 1:1 was ranged from 310-610 nm (table 4) , (F15- F20) of drug : stabilizer ratio 1:2 was ranged from 146-544 nm (Table 5). At ratio1:2 drug : stabilizer large particle size show in combination of poloxamer 188 and PVP k-90 gave higher size than alone that show in F16 (544nm). In F11that contain poloxamer 188 alone get particle size 383 nm and in F13 that contain PVP k-90 alone get particle size 379 nm that mean PVP has a higher affinity to adsorb mirtazapine than Poloxamer 188, these results due to that the combination lead to increase viscosity of the disperse media, so it is ineffective combination and cannot stabilize the nanoparticulate system (26). Nanoparticles formulation generally requires addition of appropriate stabilizers to lower the free surface energy of the nanoparticles and prevent particle aggregation and/or particle growth. The high surface free energy of nanoparticles is readily lowered by lowering the solid–liquid interfacial tension upon addition of surfactants(24). The formula F15 showed lowest PDI (0.021), as seen in table 5 at drug : stabilizer ratio 1:2, that indicate good uniformity of nanoparticle size. Uniformity of particle size is determined by polydispersity index values in which the low value means the best uniformity when used two stabilizer (poloxamer 188 + poloxamer 407). Iraqi J Pharm Sci, Vol.29(1) 2020 Mirtazapine nanoparticles 67 Table 4. Particle Size , PDI and EE% of formulas at drug: stabilizer ratio 1:1. Formula Stabilizers Particles size PDI EE% F1 Poloxamer 188 429 0.171 83.9 F2 Poloxamer407 444 0.214 83.3 F3 PVP-k90 460 0.187 78.2 F4 PVA 691 0.293 85.3 F5 Poloxamer188+Poloxamer407 342 0.178 89.3 F6 Poloxamer188+PVP-k90 610 0.420 78.7 F7 Poloxamer188+PVA 492 0.123 88 F8 Poloxamer407+PVP-k90 488 0.331 86.5 F9 Poloxamer407+PVA 325 0.142 89.6 F10 PVP-k90+PVA 310 0.029 89.6 Table 5. Particle size, PDI and EE% of formulas at drug: stabilizer ratio 1:2. EE% PDI Particles size Stabilizers Formula 88.2 0.079 383 Poloxamer188 F11 93.7 0.192 401 Poloxamer407 F12 89.2 0.113 379 PVP-k90 F13 87.8 0.051 572 PVA F14 95.9 0.021 146 Poloxamer188+Poloxamer407 F15 79.6 0.341 544 Poloxamer188+PVP-k90 F16 87.8 0.132 381 Poloxamer188+PVA F17 87.2 0.038 338 Poloxamer407+PVP-k90 F18 88.7 0.188 239 Poloxamer407+PVA F19 89.7 0.114 208 PVP-k90+PVA F20 Determination of drug entrapment efficiency of nanosuspension (EE%) The EE% of the formulations from 78.2% – 95.9 % (Table 4,5) The drug entrapment efficiency of F15was high when compared to other formulations. In present work, a relatively high %EE (95.9) in F15 was obtained for most of the prepared mirtazapine nanosuspension formulas which may be attributed higher affinity towards the lipid matrix due to its lipophilic partition coefficient (27). They represent integral parameters in the formulation due to their influence on drug release characteristics and therefore its bioavailability to the biological system. Hydrophobic drug molecules are easier to be incorporated in nanoparticles with higher efficiency relative to hydrophilic drugs due to the later tendency to partition into the aqueous phase-out of the lipid phase during homogenization(28). In-vitro drug release study of mirtazapine nanosuspension In vitro dissolution study was performed for all formulas using USP dissolution test apparatus-II. In 0.1N HCl and in phosphate buffer solution (pH 6.8) media showed the F15 that contain poloxamer 188 and poloxamer 407 stabilizers gave the best release when comparison with other formulas and the formula shows a maximum cumulative percentage drug release of 99.9% within 20 min. The release of F15 in media of 0.1N HCl and in phosphate buffer pH6.8, the maximum cumulative percentage drug release reach to 99.9 % within 20 minutes and in phosphate buffer solution (pH 6.8) release of F15 reach 90.2 % in 40 minutes(29) (Figure1). Iraqi J Pharm Sci, Vol.29(1) 2020 Mirtazapine nanoparticles 68 The release of F15 was compared with the pure drug in media of 0.1N HCl (Figures 2) the maximum cumulative percentage drug release of F15 was 99.9 % within 20 minutes, whereas the pure drug having a release of 93.2 % within 60 minutes. The obtained results are in good accordance with Noyes–Whitney equation which states that the increase in saturation solubility and the decrease in particle size lead to an increased dissolution rate(30). Figure 1. Dissolution profile of mirtazapine ( F15) nanosuspension in 0.1 N HCl (pH 1.2) and in phosphate buffer (pH 6.8) at 37C . Figure 2. Dissolution profile of mirtazapine (F15) nanosuspension and pure drug in 0.1N HCl (pH 1.2) at 37C Drug content in lyophilized powder The drug content result of lyophilized powder of the selected formula (F 15) 97.64% of mirtazapine when determined by UV-visible spectrophotometer at λmax 315 nm. Evaluation of mirtazapine nanoparticles powder Powder flowability Angle of repose and compressibility index of the powder of the formulas (F15a and F15b) were reported in Table (6). Table 6. Flow properties of prepared blends of mirtazapine incorporating drug nanoparticles. Formula Angle of repose Carr,s index Hausner ratio Physical property Angle of repose Carr,s index F15a 13.6 11.4 1.16 Excellent good F15b 21 27.5 1.34 good poor Evaluation of mirtazapine tablets The mechanical properties of pharmaceutical tablets are quantifiable by the friability, hardness or crushing strength. The hardness of all the formulas as shown in Table (7) had an acceptable values 7, 6.5 kg/cm2. The hardness of F15a containing MCC (Avicel)® 82 mg was 7kg /cm2 larger than F15b . During compression, MCC (Avicel)® PH 102 is believed to undergo stress relief deformation by several mechanisms, this might be attributed to the hydrogen bonds formed among the hydroxyl groups of the adjacent cellulose particles of (Avicel)®, which are brought closely together by plastic deformation during compression, so that it produces hard tablets at low compression forces(31). The loss in total weight of the tablets due to friability was found in all formulation, which indicated to be less than 1% for friability and which confirms the mechanical stability of tablets(32). Physical properties of the prepared tablets, weight variation and drug content, demonstrated in Table (7). The weight variation of F15a, F15b was within the pharmacopoeia limits which is ± 7.5% of the average weight. Weight variation of the prepared tablets was within the limit (149.2 mg, 147.9 mg ) and this indicates that there is no deviation from the limit of 7.5% of USP pharmacopoeia limits(33). The content uniformity of the prepared formulas was within the accepted pharmacopeia limits (85% - 115%) and this mean that all the formulations revealed good uniformity and had yielded results from 101% , 98.7 respectively. Disintegration time of prepared tablets about 10 sec. and 13 sec. Table 7. Mechanical strength and physical properties of the prepared mirtazapine incorporating drug nanoparticles Formula Hardness (kg/cm2) Friability % Weight variation (mg) Drug content (%) Disintegration time (sec.) F15a 7 0.45 149.2 101 10 F15b 6.5 0.67 147.9 98.7 13 Iraqi J Pharm Sci, Vol.29(1) 2020 Mirtazapine nanoparticles 69 In Vitro dissolution study of tablet The release profiles of the prepared mirtazapine tablets incorporating drug nanoparticles (F15a, F15b) and tablet marketing of mirtazapine as a reference were tested in 0.1N HCl (pH 1.2) and phosphate buffer solution (pH 6.8) as shown in figures (3) and (4), F15a was faster compared with F15b and the marketed tablet of mirtazapine. Figure 3. Dissolution profile of prepared tablets and mirtazapine marketed in buffer (pH 1.2) at 50 r.p.m and 37ºC . Figure 4. Dissolution profile of prepared tablets and mirtazapin marketed in buffer (pH 6.8) at 50 r.p.m and 37ºC. Fourier transform infrared spectroscopy (FTIR) FTIR is one of the most widely reported spectroscopic techniques for solid-state characterization. IR spectroscopy of mirtazapine (Figure 5), N-H stretching 3245 cm-1, Methyl group attached to a N2 atom gives rise to a band at 2854 cm-1 , Bands for C-C stretching of the phenyl group appeared at 1585 cm-1 and 1444 cm-1. The primary aromatic amines with N directly on the ring give bands at 1336-1200 cm-1. The benzene ring C-H appears in the range of 1359-1074 cm-1 and 788-636 cm-1 for the in plane and out of plane bending vibrations respectively (34, 35). The characteristic bands of mirtazapine as lyophilized powder, blend powder of best formula (F15a) show The benzene ring C-H appears 1084 cm-1, C-H stretching vibrations band of methyl group at 3101.94 cm-1, Bands for C-C stretching of the phenyl group appeared at 1640 cm-1, N-H stretching peak show between (3101- 3369) cm-1. It was observed that there were no changes in these main peaks in the FTIR spectra of a mixture of drug and excipients. The FTIR study demonstrate that no physical or chemical interactions of Mirtazapine with other excipients. These are the main characteristic absorption band show in figure (6,7). Figure 5.FT–IR spectra of mirtazapine pure powder Iraqi J Pharm Sci, Vol.29(1) 2020 Mirtazapine nanoparticles 70 Figure 6. FTIR spectrum of lyophilized powder Figure 7. FTIR spectrum of blend powder best formula(F15a) Differential Scanning Calorimetry Figure (8) demonstrate DSC of mirtazapine showed sharp characteristic endothermic peak at 117ºC and this agrees with published results. This gives an indication that the drug has crystalline nature with high purity. For lyophilized powder, the melting point of mirtazapine disappeared as in figure (9) giving a strong indication that the drug lost the crystallinity state and converted to an amorphous form(36) . Iraqi J Pharm Sci, Vol.29(1) 2020 Mirtazapine nanoparticles 71 100.00 200.00 Temp [C] -5.00 0.00 5.00 mW DSC 117.31x100C Figure 8. DSC thermogram of mirtazapine pure powder Figure9. DSC thermogram of lyophilized powder Analytical RP-HPLC Method Assay for mirtazapine was determined using HPLC technology to be compared with the UV spectroscopy. Figure (10) shows the HPLC chromatogram of mirtazapine as pure powder in the mobile phase. [The retention time of mirtazapine in the HPLC chromatogram was 7.141 minutes , for lyophilized powder of mirtazapine nanosuspension for best formula (F15) the retention time in the HPLC chromatogragram was 7.129 minutes, as shown in figure (11)]. From the results it was found that no significant difference between the two methods for the assay of mirtazapine pure powder and mirtazapine lyophilized powder. Iraqi J Pharm Sci, Vol.29(1) 2020 Mirtazapine nanoparticles 72 Figure 10. HPLC chromatogram of mirtazapine pure powder Figure 11. HPLC chromatogram of lyophilized powder for mirtazapine in the selected formula, f15 Evaluation of surface morphology Atomic Force Microscopy Study (AFM) AFM is akind of scanning probe microscopes (SPM). It is an instrument that measure the properties of surfaces. AFM is capable of scanning the surfaces in controlled environmental conditions and is complementary to SEM imaging. With the high precision of the AFM, in principle it is possible to determine the dimensions of nanoparticles with high accuracy. AFM allows the visualization of samples with resolution in three dimensions x-, y- and z-directions in atmospheric or submerged conditions. The morphological analysis of mirtazapine pure powder performed by AFM showing spherical shaped nanoparticles Figure (12) . It was found to be Iraqi J Pharm Sci, Vol.29(1) 2020 Mirtazapine nanoparticles 73 stable and no aggregation of particles could be observed (37). The formulation was found to be stable and no aggregation of particles could be observed. The particle size of F15 obtained by AFM was comparable to or equal to that measured by ABT- 9000 nano laser and this agreement in particle size measurements provide the good size distribution and the stability of mirtazapine nanoparticles(38), as show in figure (13). Figure12. AFM of mirtazapine pure powder Figure 13. AFM of F15 Conclusion Nano particulate systems such as anti- solvent precipitation have a great potential method, being able to convert poorly soluble mirtazapine. Mirtazapine nanoparticles were successfully prepared using different types of stabilizers alone and combination of stabilizers at drug : stabilizer ratios 1:1 and 1:2. Drug : stabilizer ratio 1:1 was effective to stabilize mirtazapine nanoparticles and the particle size was decrease as the stabilizer concentration increase. The selected formula F15, containing poloxamer 188 and poloxamer 407 as stabilizers combination, showed good entrapment efficiency of 93 % and faster dissolution rate than other formulas and pure drug. Selected formula Iraqi J Pharm Sci, Vol.29(1) 2020 Mirtazapine nanoparticles 74 F15a was prepared as an orodispersible tablet by direct compression method and characterized by acceptable hardness, low friability and produced higher dissolution rate in comparison with the marketed tablet. References 1. Sikarra D, Shukla V, Kharia A, Chatterjee P. Techniques for solubility enhancement of poorly soluble drugs: An overview, Journal of Medical Pharmaceutical and Allied Sciences 2012; 1: 1- 22. 2. Merin M, Krishnakumar K, Dineshkumar B, Smitha N. Antibiotics nanosuspension: A review. Journal of Drug Delivery and Therapeutics. 2017; 7(2): 128- 31. 3. Hetal PT, Arpita A P, Nirav PC. Formulation and optimization of mucoadhesive microemulsion containing mirtazapine for intranasal delivery. Chronicles of Young Science. 2014; 5(1): 25- 32. 4. Kenneth ME, Chika JM, Patience O, Rui K. Pharmacokinetics studies of mirtazapine loaded nanoemulsion and its evaluation as transdermal delivery system. Journal of Chemical and Pharmaceutical Research. 2017; 9(3): 74- 84. 5. Abirami1 M, Raja J, Mekala P, Visha P. Preparation and characterisation of nanocurcumin suspension. International Journal of Science, Environment and Technology. 2018; 7(1):100-103. 6. Jassim ZE, Hussein AA. Formulation and evaluation of clopidogrel tablet incorporating drug nanoparticles. International Journal Pharmacy and Pharmaceutical Science. 2014; 6(1): 838- 845. 7. Vijay KS, Preeti S, Dinesh C. Formulation and evaluation of effect of different stabilizer at nanosuspension of satranidazole .World Journal of Pharmacy and Pharmaceutical Sciences 2014; 3(2): 1367-1377. 8. Shailaja k, Naga R, Deepika B, Regupathi T. Formulation and in vitro evaluation of dissolving tablets of mirtazapine using sublimation method. Innovate International Journal of Medical and Pharmaceutical Sciences. 2017; 2(1): 48- 55. 9. Amol K, Satyendra M, Ravindra K, Jitendra N. Formulation and evaluation of glipizide loaded nanoparticles. International Journal of Pharmacy and Pharmaceutical Sciences . 2013; 5(4): 147- 151. 10. Manal KD. Application of Quality by Design principles to study the effect of co-processed materials in the preparation of mirtazapine orodispersible tablets. International Journal of Drug Delivery . 2013; 5(3): 309- 322. 11. Christoph K, Veronika B, Atsutoshi I, Jochen S, Geoffrey L. Formation of Mefenamic Acid nanocrystals with improved dissolution characteristics. Chemical Ingredient technique. 2017; 89(8):1060- 1071. 12. Prashant BP, Avinash GM, Kuldip HR, Y P Sharma, Sagar NP. Mouth dissolving tablet: A review. International Journal of Herbal Drug Research. 2011; 1(2): 22- 29. 13. Haritha B. A review on evaluation of tablets. Journal of Formulation Science and Bioavailability. 2017; 1: 1-5. 14. Dandan L, Heming X, Baocheng T, Kun Y, Hao P, Shilin M, Xinggang Y, Weisan P. Fabrication of carvedilol nanosuspensions through the anti-solvent precipitation– ultrasonication method for the improvement of dissolution rate and oral bioavailability. APS Pharmaceutical Sciences Technology. 2012; 13(1):295– 304. 15. Prakash S, Suryadevera V , Anne R , Reddyvalam L , Kunam V. Development and characterization of a novel nanosuspension based drug delivery system of valsartan: A poorly soluble drug. Asian Journal of Pharmaceutics. January-March 2015; 9(1): 29- 34. 16. Randa MZ, Adel AA . Formulation and In- vitro evaluation of diacerein loaded niosomes. International Journal Pharmacy and Pharmaceutical Science. 2014; 6(2): 515–521. 17. Chai F, Sun L, Ding Y, Liu X, Zhang Y, Webster TJ, Zheng C. A solid Self- Nanoemulsifying system of the bcs class iib drug dabigatran etexilate to improve oral bioavailability. Nanomedicine (Lond.). 2016;11(14): 1801-1816 . 18. Manickam B, Shyam SA. Formulation and evaluation of chitosan based bioadhesive drug delivery systems of lisinopril for prolonged drug delivery. Pelagia Research Library. 2013; 4(3):1- 7. 19. Dhiman S, Thakur GS. Nanosuspension: A recent approach for nano drug delivery system. International Journal Current Pharmaceutical Research. 2011; 34(1): 96-101. 20. Mishra B, Arya N, Tiwari S. Investigation of formulation variables affecting the properties of lamotrigine nanosuspension using fractional factorial design. Daru. 2010;18(1):1-8. 21. LiuySun C, Haoy T, Zheng L, Wang S. Mechanism of dissolution enhancement and bioavailability of poorly water soluble celecoxib by preparing stable amorphous nanoparticles. Journal of Pharmacy and Pharmaceutical Sciences. 2010;13(4):589- 606. 22. Zuy SW, Zhao X, Wang W, Liyge Y. Preparation and characterization of amorphous amphotericin b nanoparticles for oral administration through liquid anti - solvent precipitation. Euroupe Journal Pharmaceutical Science. 2014; 53(1): 109- 117. https://www.ncbi.nlm.nih.gov/pubmed/?term=Liu%20D%5BAuthor%5D&cauthor=true&cauthor_uid=22246736 https://www.ncbi.nlm.nih.gov/pubmed/?term=Xu%20H%5BAuthor%5D&cauthor=true&cauthor_uid=22246736 https://www.ncbi.nlm.nih.gov/pubmed/?term=Tian%20B%5BAuthor%5D&cauthor=true&cauthor_uid=22246736 https://www.ncbi.nlm.nih.gov/pubmed/?term=Yuan%20K%5BAuthor%5D&cauthor=true&cauthor_uid=22246736 https://www.ncbi.nlm.nih.gov/pubmed/?term=Yuan%20K%5BAuthor%5D&cauthor=true&cauthor_uid=22246736 https://www.ncbi.nlm.nih.gov/pubmed/?term=Pan%20H%5BAuthor%5D&cauthor=true&cauthor_uid=22246736 https://www.ncbi.nlm.nih.gov/pubmed/?term=Ma%20S%5BAuthor%5D&cauthor=true&cauthor_uid=22246736 https://www.ncbi.nlm.nih.gov/pubmed/?term=Yang%20X%5BAuthor%5D&cauthor=true&cauthor_uid=22246736 https://www.ncbi.nlm.nih.gov/pubmed/?term=Pan%20W%5BAuthor%5D&cauthor=true&cauthor_uid=22246736 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3299468/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3299468/ Iraqi J Pharm Sci, Vol.29(1) 2020 Mirtazapine nanoparticles 75 23. Dora CP, Singh SK, Kumar S, Datusalia AK, Deep A. Development and characterization of nanoparticles of glibenclamide by solvent displacement method. Acta Polonia Pharmceutical , 2010; 67(3): 283- 290. 24. Cerdeira AM, Mazzotti M, Gander B. Formulation and drying of miconazole and itraconazole nanosuspensions. International Journal of Pharmaceutics. 2013; 443: 209- 220. 25. Wu L, Zhang J, Watanabe W. Physical and chemical stability of drug nanoparticles. Advanced Drug Delivery Reviews. 2011; 63(6): 456-469. 26. Lee J, Choi JY, Park CH. Characteristics of polymers enabling nano-comminution of water insoluble drugs. International Journal Pharmaceutical. 2008; 355: 328– 336. 27. Merck M, Maryadele J, Smith E. Heckelman S. The Merck Index: An Encyclopedia of Chemicals, Drugs, and Biologicals, Fifteenth Edition, Royal Society of Chemistry, 2013;p502. 28. Jyotsana RM, Priyanka AK, and Kamal D, Development and evaluation of solid lipid nanoparticles of mometasone furoate for topical delivery,Internatioonal Journal of Pharmaceutical Application. April 2014; 4(2). 29. Richard W. Method for Delivering an Active Ingredient by Controlled Time Release Utilizing a novel Delivery Vehicle Which can be Prepared by a Process Utilizing the Active Ingredient as a Porogen. United States Patent. 1987; 4690825. 30. Bansal S, Bansal M, Kumria R. nanocrystals :current strategies and trends. International Journal of Research in Pharmaceutical and Biomedical Sciences. 2012; 3(1): 406- 419. 31. Selvaraj B, Malarvizhi P, Shanmug P. Development and in-vitro characterization of tramadol hydrochloride sustained release tablets. International Journal of Pharmaceutical Technology Research. 2013; 5(2): 492-500. 32. Saigal N, Baboota S, Ahuja A, Ali J. Microcrystalline cellulose as a versatile excipient in drug research. Journal of Young Pharmacists. 2009; 1(1): 1-6. 33. Gad SC. Pharmaceutical Manufacturing Handbook: Production and Processes: Wiley- Interscience; 2008. 34. Seda G, Ayse E. Molecular Structure, FTIR, FT-Raman, NMR Studies and first order molecular hyper-polarizabilities by the dft method of mirtazapine and its comparison with mianserin. Spectroscopy Chemical and Bio- Molecular Spectroscopies. 2013;104(1):222- 234. 35. Mehta MR , khawala CK, Patel NC. Formulation and evaluation of quick dissolving film of mirtazapine. International Journal of Pharmaceutical Research and Bio- Science. 2014; 3(2): 950- 968. 36. AL- Majed A, Bakhiet AH, AL- Harbi RM, Abed AL- Aziz HA. Profiles of drug substances, excipients, and related methodology, Mirtazapine. 2018; 43(1): 209- 249. 37. Leedy H. 3-Dimensional profile distortion measured by stylus type su clark profilometer. Measurement.2013; 4(6): 803– 814. 38. Bailey N. Characterizations of drug nanoparticles by atomic force microscopy. 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