P U B L I C A T I O N S CODON ISSN 1120-1770 online, DOI 10.15586/ijfs.v33iSP1.2088 137 P U B L I C A T I O N S CODON Underutilized horse chestnut (Aesculus indica) flour and its utilization for the development of gluten-free pasta Syed Insha Rafiq1, Khalid Muzaffar2, Syed Mansha Rafiq3, DC Saxena1*, BN Dar2 1Department of Food Engineering and Technology, SLIET, Longowal, Punjab, India; 2Department of Food Technology, IUST, Awantipora, India; 3Department of Food Science & Technology, NIFTEM Kundli, Sonepat, Haryana, India *Corresponding Authors: DC Saxena, Department of Food Engineering and Technology, SLIET, Longowal, Punjab India. Email: dcsaxena@yahoo.com; BN Dar, Department of Food Technology, IUST, Awantipora, India. Email: darnabi@gmail.com Received: 11 June 2021; Accepted: 18 September 2021; Published: 7 October 2021 © 2021 Codon Publications OPEN ACCESS PAPER Abstract There has been a growing demand for the production of gluten-free products due to increased occurrence of celiac disease. Thus, different research groups have been investigating the use of various available materials for the development of these functional products to fulfill customer’s needs. Horse Chestnut (Aesculus indica) seeds are underutilized, low-cost, and gluten-free, found in hilly areas of the Himalayan region of Kashmir valley, India. To determine their potential as an alternative to conventional food grains, an investigation was conducted to determine the physicochemical, functional, pasting, and thermal properties of Horse Chestnut (HCN) flour and its compatibility for the development of gluten-free pasta. HCN flour comprised 73.34% carbohydrate, 11.36% protein, 6.34% crude fiber, 3.27% fat, 3.16% ash, 3.15g/g oil absorption capacity, and 4.65% water absorption index. HCN flour showed 505 cp peak, 354 cp trough, 151 cp breakdown, 472 cp final viscosity, and 66.05°C pasting tem- perature. Transition temperatures (onset, peak, and conclusion) and enthalpy change (ΔH) were 60.12°C, 69.90°C, 81.53°C, and 10.56 J/g, respectively. Pasta prepared from HCN flour using guar gum (0, 0.5, and 1%) was ana- lyzed for color, cooking qualities, and textural and sensory analysis. The present results showed that HCN flour possesses good nutritional quality and has properties comparable to conventional wheat flour. Therefore, HCN- flour-based pasta can act as a nutritious alternative to conventional gluten-free pasta and add variety to the diet of people suffering from celiac disease. Keywords: celiac disease; cooking; horse chestnut; nonconventional; nutritional; pasta Introduction Horse Chestnut (Aesculus indica) is abundantly found in hilly areas of the Himalayan region of Kashmir val- ley, India. The seeds of the tree are harvested during October and November. Horse Chestnut (HCN) seeds are mainly consumed by wild animals and many seeds are wasted. The seeds are kept in running water to reduce the bitterness attributed to the presence of anti-nutri- ents and then added in powdered form to wheat flour for making Halwa (porridge) and chapattis, a traditional flatbread (Rajeasekaran and Joginder, 2009). HCN seeds can be used as nutritional supplements because they are nutritionally good and have a distinctive composition of carbohydrate, crude protein, fat, and ash. Oleic acid, linoleic acid, palmitic acid, linolenic acid, arachidic acid, and myristic acid are the major fatty acids present in the HCN seed. The chief minerals present in HCN include potassium, phosphorus, calcium, sulfur, iron, copper, zinc, and manganese (Majeed et al., 2010). The seeds Italian Journal of Food Science, 2021; 33 (SP1): 137–149 mailto:dcsaxena@yahoo.com mailto:darnabi@gmail.com 138 Italian Journal of Food Science, 2021; 33 (SP1) Rafiq SI et al. improve their textural characteristics (Lorenzo et  al., 2018; Palavecino et al., 2017). To improve the quality attributes like mouthfeel, texture, consumer acceptabil- ity, and shelf-life of gluten-free products, diverse research has been done using alternative components like hydro- colloids, emulsifiers, starches, and nongluten proteins (Capriles et al., 2016; Kumar et al., 2019). The worldwide market for gluten-free food products increased up to 83% with an estimated annual growth rate of 12.3% for pasta products until 2022 (Chauvin, 2019). Gluten-free products are not only consumed by celiac patients but also by other individuals as well (Bresciani et al., 2021). Researchers have been motivated by United Nations for the Agenda 2030 to research healthy formulations for bakery and pasta products and the use of functional agro-industrial by-products for fortification in foods (Simonato, 2021). Because of its good nutritional profile and gluten-free nature, HCN flour may represent a potential noncon- ventional alternative to conventional flours. Only limited information is available regarding HCN flour and there are no reports about the production of gluten-free pasta from HCN four. Therefore, it is essential to characterize the flour to explore possible utilization in food industry and to optimize the ingredients for the development of quality pasta from HCN flour. So, in the present study, charac- terization of HCN flour with respect to physicochemical, functional, thermal, and pasting properties, and develop- ment and characterization of pasta were carried out. Materials and Methods Raw materials Fully matured Horse Chestnut seeds were obtained from Horse chestnut trees located at Anantnag, Jammu & Kashmir, India, cleaned and stored at 5°C till used. Hard wheat was procured from Punjab Agriculture University (Ludhiana). The chemicals and reagents were procured from Sigma-Aldrich. Flour preparation Horse Chestnut flour was prepared with slight modifi- cation to the method of Singh et al. (2013). The kernels obtained after manual dehulling of HCN seeds were sliced and then dried in a tray dryer at 60°C. The slices before drying were treated to reduce the anti-nutritional factors in the previous work of RAFIQ et al. (2016). The dried seed slices were ground in a laboratory-type grinder to obtain HCN flour. Both wheat and HCN flours were sieved using a 60-mesh sieve. HCN and wheat flour were then analyzed for various properties. also have several medicinal uses like in the treatment of fever, viral diseases, skin infections, and cardiovascular disorders (Kaur et al., 2011). HCN seeds are rich in poly- phenols, flavonoids, fiber, and essential minerals (Majeed et al., 2010). Pasta, a traditional delicacy, is a popular staple food used throughout the world because of its low cost, lon- ger shelf life, and ease of preparation. Commonly, wheat flour is used in the preparation of pasta (Kaur et al., 2012). Pasta food products are produced by extruding feed mixture in an extruder equipped with different dyes that determine the shape of the final product. The pasta products are then dried and packed. It has been recog- nized as an ancient means of nourishment and versatile food from nutritive and gastronomic perspectives (Fuad and Prabhasankar, 2010). Pasta is considered a superior medium for the incorporation of nutrients as per the reports of Food and Drug Administration and World Health Organization (Pasqualone et al., 2016). The selec- tion of flour for pasta development depends on water absorption and water solubility indexes and the accept- ability and cooking parameters of the developed pasta (Rudra et al., 2020). The behavior of starch and protein in pasta during cooking is such that starch swells and solubilizes but protein gets coagulated during cooking (Hager et al., 2012). Best-quality pasta has acceptable firmness with low solid loss, and stickiness (Phongthai et al., 2017). Gluten as the structure builder promotes viscosity, elasticity, extensibility, and cohesiveness in pasta products (Lazaridou et al., 2007). Gluten helps to maintain the shape of bakery products and pasta due to the extensibility and elastic dough behavior with resil- ience and textural properties acceptable to consumers (Phongthai et al., 2017). However, due to the amplified dominance of celiac disease and other health issues such as gluten allergy, nonceliac gluten sensitivity, and other gluten-related disorders, demand for gluten-free pasta products has increased (Dib et al., 2018; Ozgoren and Yapar, 2019). To avoid these disorders, grains other than wheat may be used, either individually or in combined formulation, to replace gluten in food products. The only possible treatment for individuals having celiac disease is to consume foods devoid of gluten (Witczak et al., 2017). However, gluten-free products have low nutritional qual- ity, and defective textural and sensory property due to crumbly and fragile dough formation (Cai et al., 2016). Hence, to counterbalance the absence of gluten, fortifi- cation with gums, proteins, and different flours are con- sidered (Foschia et al., 2017; Padalino et al., 2016). To replace gluten and to maintain the dough structure and texture of the final product, various types of protein and hydrocolloids are used in gluten-free products (Foschia et al., 2017). Hydrocolloids, such as xanthan gum, guar gum, hydroxypropyl methylcellulose, and carboxymethyl cellulose used as an addictive in gluten free products can Italian Journal of Food Science, 2021; 33 (SP1) 139 Underutilized horse chestnut (Aesculus indica) flour and its utilization sample was weighed to calculate OAC as shown in the below equation: Weight of residue OAC (g / g) Sample weight = Emulsion properties The emulsion activity and stability of samples were cal- culated by using the method of Du et al. (2014). Sample (3.5  g) and water (50 mL) were agitated for 30 s using the high setting homogenizer (Yorco, India). Afterward, groundnut oil (25 mL) was added and then again homog- enized for 30 s, followed by another addition of 25 mL groundnut oil and then homogenized for 90 s. The emul- sion obtained was then centrifuged for 5 min at 1100 × g. Emulsion activity (%) Volume of emulsified layer Emu Emulsion volume before centrifugation = × After calculating the emulsion activity, emulsion stabil- ity was evaluated by heating the flour emulsion samples (15  min at 85ºC), then cooling, followed by centrifuga- tion for 5 min at 1100 × g. The percent emulsion activ- ity that remained after heating can be expressed as the emulsion stability. Foaming properties The foaming properties, viz., foaming capacity (FC) and foaming stability (FS), were observed with minor mod- ifications to the method followed by Jia et al. (2021). Sample (3 g) and distilled water (50 mL) were mixed, and the dispersion was homogenized for 3–5 min. The mix- ture was then instantly poured into the measuring cylin- der and the increase in volume percent upon whipping was calculated. The percentage of volume increase was the foaming capacity and the change in the foam volume after 1 h of storage was recorded as the foaming stability. FC and FS were calculated using the below mentioned formulas: 2 1 2 3 2 1 V V FC(%) 100 V V FS(%) 100 V V − = × = × − where, V1 = volume prior to whipping (mL), V2 = foam volume after whipping (mL), and V3 = foam volume (mL) after holding Least gelation concentration (LGC) The method of Khan and Saini (2016) was used to deter- mine the gelation properties of the flour samples. Two Physicochemical characteristics of HCN flour Color analysis Flour color analysis was done in triplicates, with Color Flex Spectro-colorimeter (D-25, Ruston, USA), and color values (L*, a*, and b*) were determined. Proximate analysis Standard methods were employed to analyze the flour samples for moisture, protein, ash, fiber, and fat con- tent (AOAC, 2006), while the carbohydrate content was determined using the difference method. Bulk density An accurately weighed sample was poured into a grad- uated cylinder. Tapping during the filling was done for uniform packing. Bulk density was calculated using the below equation: 3 3 Weight of the sample (g) Bulk density (g / cm ) Volume of sample (cm ) = True density The method followed by Raigar and Mishra (2015) was used to determine true density. An accurately weighed flour sample (1 g) was taken and poured into a burette filled with toluene. The equation given below was used to calculate true density: 3 3 Weight of flour sample (g) True density (g / cm ) Rise in toluene level (cm ) = Functional properties Water absorption capacity Water absorption capacity (WAC) of the flour was deter- mined per Vázquez-Luna et al. (2019) with slight modifi- cations. Approximately, 5 g of the sample was mixed with 70 mL of water for 1 min and then held at room tempera- ture for 30 min, followed by centrifugation (3000 × g for 15 min). After centrifugation, the sediment obtained was weighed and WAC was calculated: Weight of se dim ent WAC(g / g) Weight of flour sample = Oil absorption capacity The method followed by Khan and Saini (2016) was used to determine the oil absorption capacity (OAC) of the sample. Five grams of flour and 75 mL of oil were mixed and agitated for 30 min and then centrifuged for 10 min at 3000 × g. Afterward, oil was drained while the retained 140 Italian Journal of Food Science, 2021; 33 (SP1) Rafiq SI et al. observed by squeezing the pasta between glass plates. The weight of the cooked pasta after being drained for 2  min was taken as the cooked weight (g). The cooking loss was calculated by evaporation of cooking water to dryness at 110°C overnight. Water uptake Water uptake is the difference between the weight of cooked and uncooked samples, and is expressed as uncooked pasta weight percentage (Çabuk and Yilmaz, 2020). The investigation indicated the absorption of water during the cooking process. Cooking loss The method followed by Teterycz et al. (2020) was employed for cooking loss determination. Sample (10 g) was cooked in boiling water (300 mL) and then cooking water was dried overnight (105°C). The residue left after drying was weighed and then cooking loss was calculated. Texture For determination of texture, the samples were cooked till optimum cooking time, and then drained with 10 min rest time. Four pasta strands were taken and placed under the compression plate of pasta firmness/stickiness rig. The resulting force-time curve was used to determine firmness, cohesiveness, springiness, adhesiveness, chewi- ness, and gumminess. Sensory evaluation Panel comprising 12 trained members have done the sen- sory evaluation of pasta samples; evaluated the attributes like firmness, slipperiness, chewiness, surface adhesive- ness, appearance; and determined the total score for each pasta sample. Perceived intensities were scored on a 5-point scale. Statistical analysis The experimental data was statistically analyzed by One- way ANOVA using triplicate readings for all experiments except sensory evaluation where 12 observations were averaged. Results and Discussion Physiochemical properties of HCN flour The desired color characteristics for the flour are low chroma (a*) value and high lightness (L*) value to meet the consumer preference. The color parameters and nutritional composition of HCN and wheat flour are given in Table 1. Results showed that the L* value of the flours was almost similar; however, significant difference percent, 4, 6, 8, 10, 12, 14, 16, 18, and 20% (w/v) of flour suspensions in 5 mL of water were boiled for 1 h, cooled in cold water, and kept at 4°C for 2 h. Pasting characteristics Rapid Visco Analyzer (TecMaster, Warriewood, Australia) was used for the determination of the pasting profile. A programmed heating and cooling cycle (taking sample 3 g, 14% mb; and distilled water 25 mL) was used for generating the viscosity profile. Experimental condi- tions include holding the samples for 1 min at 50°C, heat- ing to 95°C at 12°C/min cooling rate, holding for 2.5 min at 95°C), cooling to 95 to 50°C at 12°C/min heating rate, and then holding for 2 min at 50°C. Thermal properties A differential scanning calorimeter (DSC821, Mettler Toledo, Switzerland) was involved in determining ther- mal properties of the sample. The samples were prepared with flour (3mg, dwb) and water (7 µl), sealed, and kept undisturbed at room temperature for 1 h. The sample was heated at 20–120°C at a rate of 10°C/min. Pasta preparation Different pasta formulations (control sample (durum wheat); native sample (HCN flour + 0% gum); pasta for- mulation containing HCN flour and 0.5 and 1% guar gum, respectively) were mixed using Hobart benchtop mixer (5KPM50, USA) for 1 min at low speed followed by conditioning for 5 min with distilled water (30 mL). The sample was fed to a single screw extruder (Model: Dolly La Monferrina Italy) of 7 mm die diameter. Pasta was then transferred to cabinet drier for 4 h set at 60°C, reducing the moisture content to 8%, and then packed in high-density polyethylene pouches. Characterization of pasta Color parameters Color parameters of pasta were determined using a color spectrophotometer (CM-3600d, Konica Minolta, Bremen, Germany). Color analysis was done in terms of L*, a*, and b* values. Cooking properties Cooking time was evaluated by using the AACC (2000) method. Pasta sample (10 g) was cooked in water (300 mL). The optimum time of cooking is the point of dis- appearance of the inner white core of pasta and was Italian Journal of Food Science, 2021; 33 (SP1) 141 Underutilized horse chestnut (Aesculus indica) flour and its utilization Table 1. Physicochemical characteristics. Physicochemical Horse Chestnut flour Wheat flour Color L* 92.07 ± 0.49a 92.85 ± 0.28a a* 3.47 ± 0.21a 2.03 ± 0.07b b* 13.70 ± 0.26a 9.69 ± 0.15b Moisture content (%) 9.71 ± 1.23b 11.35 ± 0.54a Protein (%) 7.78 ± 1.19b 11.36 ± 1.10a Crude fat (%) 3.27 ± 0.39a 1.40 ± 0.57b Crude fiber (%) 6.34 ± 0.22a 1.67 ± 0.14b Ash (%) 3.16 ± 0.05a 0.88 ± 0.03b Carbohydrate (%) 69.74b 73.34a Energy value (Cal/100g) 327.51b 346.84a Bulk density (g/mL) 0.64 ± 0.12a 0.51 ± 0.10a True density (g/mL) 1.25 ± 0.24a 1.42 ± 0.12a Results presented are mean values and the superscripts significantly differ in row (P < 0.05). was observed in a* and b* values. This variation in the color parameters may be ascribed to a difference in the natural pigment content inherent to HCN and wheat flours depending on the origin of the plant (Drakos et al., 2017). Nutritional composition of HCN and wheat flour also varied significantly (Table 1). Moisture content of HCN flour was low (9.71%) compared to wheat flour (11.35%). Similarly, protein and carbohydrate content of HCN flour was lower than wheat flour. However, HCN flour showed significantly higher values for ash, fat, and crude fiber content than wheat flour. High ash content of HCN flour could be related to high mineral content of the HCN seeds being rich in nitrogen, potassium, phos- phorus, sulfur, calcium, iron, etc. (Majeed et al., 2010). The bulk density did not differ significantly in both the flours (Table 1). Similar results were observed for the true density of HCN flour (1.25 g/mL) and wheat flour (1.42 g/mL). Functional properties of HCN flour Water absorption capacity WAC describes the interaction of flour and water under a limited quantity of water. The WAC of flour plays a significant part in food research as it affects other deter- mining properties of food material. HCN flour showed significantly lower values of WAC, 2.21 g/g as compared to wheat flour, 4.65 g/g (Table 2). Hydrophilic behav- ior and gel-forming ability of starch, and protein qual- ity are the determining factors that affect the WAC of flour (Al-Farga et al., 2016). Higher number of hydro- philic components in flour is responsible for its elevated WAC (Njintang et al., 2008). The variation in the WAC Table 2. Functional characteristics. Properties HCN flour Wheat flour WAC (g/g) 2.22 ± 0.12b 4.65 ± 0.08a OAC (g/g) 3.24 ± 0.15a 1.10 ± 0.05b Foaming capacity (%) 20.2 ± 1.17b 31.40 ± 0.71a Foaming stability (%) 22.32 ± 2.41b 54.05 ± 5.52a Emulsion activity (%) 58.69 ± 0.31b 61.01 ± 1.33a Emulsion stability (%) 62 ± 0.27b 68.32 ± 0.12a WAC, Water absorption capacity; OAC, Oil absorption capacity. Results presented are mean values and the superscripts significantly differ in row (P < 0.05). behavior of the flours could be related to difference in the composition of the flour. Oil absorption capacity OAC capacity is an important property of the flour, which defines its application in various food formula- tions and determines its capacity to entrap oil which affects flavor retention and mouthfeel of the developed product (Khattab and Arntfield, 2009). OAC of flour is important for new product development and storage stability. Significantly higher values of OAC of 3.15 g/g in HCN flour than 1.10 g/g in wheat flour was observed (Table 2). Interaction of nonpolar side chains present in the flour with oil through hydrocarbon side chains affect their OAC (Adebowale and Lawal, 2004). Flour particle size, starch content, and occurrence of nonpolar amino acids are other factors that affect the OAC of flour (Chau et al., 1997; Ofori et al., 2020). Also, the protein type and content contribute to oil-retaining properties of the foods (Ravi and Sushelamma, 2005). Foaming properties The foaming properties are essential parameters for the formulation of food products. Results related to foaming capacity and stability of HCN, and wheat flour is given in Table 2. Carbohydrate and protein content play an important role in determining the foaming properties of flour (Sreerama et al., 2012). Formation of interfa- cial layer by proteins is responsible for foaming, keeps air bubbles in suspension, and slows down coalescence rate (Adebowale and Lawal, 2004). HCN flour and wheat flour showed foaming capacity of 20.20 and 31.40%, respectively. Low-protein and high-fat content of HCN flour is responsible for lower foaming capacity of HCN flour (Siddiq et al., 2010). Formation of the protein-fat complex may influence the foaming properties. Foam stability for Horse Chestnut flour (22.32%) was also lower than wheat flour (54.05%). Good foam stability is most likely associated with soluble proteins’ surface activity 142 Italian Journal of Food Science, 2021; 33 (SP1) Rafiq SI et al. to swelling that is responsible for the low PT of Horse Chestnut flour. The pasting temperature has also been related to the amount of protein present. Shevkani et al. (2015) have reported high pasting temperature with higher amount of protein in rice flour. Peak viscosity (PV) which indicates the degree of granule swelling was 505 cP for HCN flour and 1215 cP for wheat flour. The low peak viscosity could be due to high fat content in HCN flour that produces more fluid system and subsequently low viscosity. Trough viscosity was 354 cP (HCN flour) and 1005 cP (wheat flour) and depicts a decline follow- ing PV owing to swollen starch granules disruption upon high temperature and shear. Breakdown viscosity was 151 cp for HCN flour and 210 cp for wheat flour. The low breakdown viscosity indicates higher resistance to shear- ing at high temperature. Final viscosity (FV) was 472 cP for HCN flour and 1560 cP for wheat flour. Besides this, setback viscosity (SBV) of HCN flour (118 cp) was con- siderably lower than that of wheat flour (301 cP). The important factors that affect setback and final viscosities are polymerization or reordering of leached amylose and linear amylopectin (Abu et al., 2006). The difference in pasting parameters of the flours could be linked to the occurrence of components such as proteins, minerals, lipids, etc., which can bind with starch, thereby affecting the pasting parameters of the flour (Ocheme et al., 2018). The protein and lipid components of the flour affect the pasting properties through amylose–protein and amylose– lipid interactions (Yu et al., 2012). Due to the protective effect of protein molecules on the integrity of starch granules, viscosity of corn starch paste decreased (Singh et al., 2014). Difference in the viscosity of wheat flour pastes is correlated to variation in the proportion of polymeric or monomeric proteins among wheat flours (Singh et al., 2016). Thermal properties of HCN flour Thermal properties are related to gelatinization behavior described as granular disruption, loss of in continuous water phase while the presence of highly ordered globular proteins in the flour which resist sur- face denaturation could be related to low foamability (Du et al., 2014). Emulsion properties The ability of protein to form a stable emulsion is known as emulsion activity, and it gives information about the ability of protein to occupy the interfacial layer of oil– water emulsion while the emulsion stability is related to the strength of an emulsion to stress, provided by the proteins (Singh et al., 2010). Emulsion capacity and sta- bility values of HCN and wheat flour are significantly dif- ferent (Table 2). Emulsion activity and stability of HCN flour was lower (58.69 and 62%) than wheat flour (61.01 and 67.87%), which might be related to the difference in quality and content of protein. Proteins having several polar side chains make them more hydrophilic, thereby increasing the protein–water interactions, and thus affecting their emulsification properties (Yan et al., 2020). The high emulsion stability depends on the globular nature of proteins in the flour. The emulsifying proper- ties of different foods vary due to varied composition and processing conditions and the capacity of protein to form and stabilize emulsions (Adebowale et al., 2005). Least gelation concentration LGC is expressed as the quantity of flour required per volume to obtain a gel. Results related to LGC of flour samples are presented in Table 3. Flour which has lower value of LGC has greater ability to form gels and low con- centration of flour is required for gel formation. Higher concentration (6 g/100 mL) of HCN flour was required for initial gel formation than wheat flour (4 g/100 mL). A firm gel was obtained when using 12% of HCN flour and 10% of wheat. The difference in the gel-forming abil- ity among various types of flours is related to different ratios of components like carbohydrates, proteins, and lipids (Drakos et al., 2017). The lower LGC value of wheat flour might be related to high protein and starch con- tent, which are responsible for gel formation (Joshi et al., 2015). Pasting characteristics Pasting profile of wheat and HCN flour is depicted in Figure 1. Pasting properties play a significant part in the selection of raw materials for industrial use as a thickener, binder, etc. Rigidity of starch granules which affects swelling of starch granules and leaching of amy- lose in solution determine the pasting properties of the flour (Leewatchararongjaroen and Anuntagool, 2016). Compared to wheat flour (pasting temperature: 83.36°C), the pasting temperature of HCN flour was low (66.05°C). It could be the starch in HCN flour that is less resistant Table 3. Least gelation concentration. Concentration HCN Gel Wheat Gel 2% No No 4% No Weak 6% Weak Weak 8% Weak Weak 10% Weak Firm 12% Firm Firm 14% Firm Firm 16% Firm Hard 18 % Hard Hard 20% Hard Hard Italian Journal of Food Science, 2021; 33 (SP1) 143 Underutilized horse chestnut (Aesculus indica) flour and its utilization double helical and crystalline structure of starch (Rincón-Londoño et al., 2016). Table 4 shows the results related to onset, peak, and conclusion temperatures, and enthalpy of gelatinization (ΔH) of HCN and wheat flour. Transition temperatures for HCN flour were To = 60.12, Tp = 69.90, and Tc = 81.53 ºC) while for wheat flour tran- sition temperatures were high (To = 78.32, Tp = 81.12, and Tc = 86.34°C). Starch granule size, their form and distribution, and the bonding between micellar network of the granule and other flour components are responsi- ble for variation in transition temperatures of flours (Xu et al., 2019). Enthalpy of gelatinization of 10.56 J/g was observed for HCN flour while for wheat flour, it was 13.21 J/g. Variation in the shape of starch granules, and propor- tion of large and small granules are the factors that affect the enthalpy of gelatinization (Kaur and Singh, 2005). The decreased gelatinization enthalpy can also be due to the disruption of crystalline regions upon depolymerization of amylose and breakdown of amylopectin. Color characteristics of pasta Customer acceptance depends on the color of the prod- uct and the most desirable attribute of pasta quality is the bright yellow color (Petitot et al., 2010). Color values (L*, a*, and b*) of raw and cooked Horse Chestnut pasta, and control were significantly different (Table 5). The L* value for Horse Chestnut pasta was less than the control pasta sample. The differences in color parameters between 1600 1500 1400 1300 1200 1100 1000 V is co ci st y 900 800 700 600 500 400 300 200 100 0 0 1 2 3 4 5 6 7 Time (Min) 8 9 10 11 12 13 14 15 Wheat HCN Flour 45 200 250 300 350 400 450 500 550 S pe ed ( rp m )600 650 700 750 800 850 900 950 Te m pe ra tu re ( ˚C ) 50 55 60 65 70 75 80 85 90 95 Figure 1. Pasting profile of wheat and HCN flour. Table 4. Thermal characteristics. Parameters Horse Chestnut flour Wheat flour Onset Temp. (°C) 60.12 ± 0.11b 78.32 ± 0.98a Peak Temp. (°C) 69.90 ± 0.23b 81.12 ± 0.12a Conclusion Temp. (°C) 81.53 ± 0.17b 86.34 ± 0.18a ΔH (J/g) 10.56 ± 0.41b 13.21 ± 0.63a Results presented are mean values and the superscripts significantly differ in row (P < 0.05). control and HCN pasta samples could be related to the inherent color of the flour and the pigments present. The addition of gum in pasta formulation at different levels showed no significant effect on pasta color. Similar effect of hydrocolloids on color characteristics of developed noodles was observed by Shere et al. (2020) Color values of cooked and uncooked pasta were significantly differ- ent which could be related to the leaching of pigments on cooking. Cooking characteristics of pasta The cooking characteristics of pasta are influenced by numerous phenomena taking place during cooking, viz., hydration, starch gelatinization, and interaction with nonstarchy constituents. Cooking parameters for the pasta samples including cooked weight, water uptake, solid loss, and cooking time are given in Table 6. The 144 Italian Journal of Food Science, 2021; 33 (SP1) Rafiq SI et al. Table 5. Color characteristics of raw and cooked pasta Sample Raw pasta Cooked Pasta L* a* b* L* a* b* A 75.40 ± 2.05a 1.30 ± 0.34b 20.73 ± 1.2 a 70.60 ± 1.68a 0.04 ± 0.01a 19.34 ± 0.76a B 62.85 ± 1.36b 1.49 ± 0.40a 18.09 ± 1.83b 55.84 ± 1.46b 0.04 ± 0.03a 16.10 ± 0.70b C 61.41 ± 1.90b 1.35 ± 0.32b 17.49 ± 1.31c 56.17 ± 2.01b 0.03 ± 0.03a 15.50 ± 0.72c D 61.82 ± 1.41b 1.38 ± 0.29ab 18.88 ± 0.53b 56.65 ± 1.61b 0.02 ± 0.02a 15.20 ± 1.61c Results presented are mean values and the superscripts significantly differ in column (P < 0.05). Sample A: control (durum wheat); Sample B: HCN seed flour; Sample C: HCN seed flour + 0.5% gum addition; Sample D: HCN seed flour + 1% gum addition. Table 6. Cooking properties of pasta. Parameters Sample (g) Cooking time (min) Cooked wt. (g/100g) Solid loss (g/100g) Water uptake (g/100g) Sample A 5 5.1 ± 0.20a 13.49 ± 0.86a 6.9 ± 0.42c 137 ± 7.21a Sample B 5 4.2 ± 0.25bc 8.57 ± 0.12c 16.8 ± 1.43a 115 ± 2.65c Sample C 5 4.5 ± 0.40ac 10.30 ± 0.90b 10.6 ± 0.52b 125 ± 3.61bc Sample D 5 4.8 ± 0.56ab 12.39 ± 0.38a 8.13 ± 0.45c 132 ± 6.56ab Sample A: control durum wheat; Sample B: native Horse Chestnut flour; Sample C: 0.5% gum addition; Sample D: 1% gum addition. Results presented are mean values and the superscripts significantly differ in column (P < 0.05) (n = 6). cooking time, cooked weight, and water uptake increased linearly (P < 0.05) with gum addition in pasta formula- tion. Results observed are in accordance with findings of Sozer et al. (2007). The enhancement in water uptake with the addition of gum might be related to the accessi- bility of carboxyl and hydroxyl groups in gum structure, thereby increasing the water uptake of the pasta sample (Gull et al., 2018). In addition, higher water uptake occurs during extended cooking time, as extra water diffuses and interacts with both protein and starch matrices. Besides this, increased affinity for water occurs because of a surge in polar amino-acid groups during cooking owing to pro- tein denaturation (Alonso et al., 2000), leading to sub- sequent increase in water uptake for pasta. The cooked weight of HCN pasta samples increased from 9.57/5 g to 12.39g/5 g with an increase in the concentration of gum used. The rise in cooked weight may be related to water-binding and water-holding capacity of the gum used for the preparation of pasta (Widelska et al., 2019). Cooking loss is regarded a vital indicator of pasta qual- ity. Cooking loss of pasta samples significantly decreased (P < 0.05) with an increase in the addition of gum con- centration from 0 to 1% in the pasta formulation. The decrease in cooking loss might be due to the addition of guar gum, which improved the protein network, formed a matrix with the proteins where starch granules get entrenched, and thus decreased the tendency of solid loss (Chauhan et al., 2017; Martin-Esparza et al., 2018). HCN pasta made with 1% gum showed comparable cooking parameters to that of the control sample. For good-qual- ity pasta, an acceptable cooking loss is around 8% (Dick and Youngs, 1988). Hence, the HCN-flour-based pasta containing 1% gum has a cooking loss in the acceptable range and could be considered as good quality. A desir- able firmness and cooking resistance, low cooking loss, and stickiness are characteristics of high-quality pasta (Lucisano et al., 2012). Studies have shown lowest cook- ing loss with improved physical and textural properties of gluten-free pasta prepared with 0.6% of xanthan gum concentration (Milde et al., 2020). Kamsiati and Herawati (2019) observed cooking loss of 5.060% and hardness of 27.530N in cassava macaroni with guar gum addition of 0.25%. Textural properties of pasta The textural assessment of pasta is an important crite- rion to estimate its quality and acceptance by consumers (Milde et al., 2020). Textural attributes of the developed pasta are presented in Table 7. The use of gum showed significant effects (P < 0.05) on the various textural attri- butes of the tested pasta samples. Firmness of the native HCN pasta sample (5.44N) was lower than the control sample (2.307N). Moreover, with the addition of gum, the firmness of HCN pasta samples increased from 2.307N to 4.297N, which might be related to an increase in the binding between protein and starch (Duda et al., 2019). Adhesiveness of HCN pasta (native) was higher than control pasta while adhesiveness of HCN pasta prepared with 1% gum addition was comparable with control. Pasta adhesiveness decreased significantly (P < 0.05) with increased gum concentration in the pasta formulation. The adhesiveness is associated with the amount of starch Italian Journal of Food Science, 2021; 33 (SP1) 145 Underutilized horse chestnut (Aesculus indica) flour and its utilization Table 7. Textural properties of cooked pasta. Parameters Sample A Sample B Sample C Sample D Hardness (N) 5.44 ± 0.63a 2.307 ± 0.10d 3.139 ± 0.22c 4.297 ± 0.42b Adhesiveness (Ns) −0.390 ± 0.02c −0.202 ± 0.07a −0.354 ± −0.27b −0.398 ± 0.09c Cohesion 0.699 ± 0.07a 0.470 ± 0.02c 0.584 ± 0.07b 0.695 ± 0.08a Springiness % 85.572 ± 2.34a 53.558 ± 1.92b 84.577 ± 3.45a 88.060 ± 3.71a Gumminess 387.654 ± 4.23a 110.460 ± 3.65d 187.047 ± 4.27c 304.454 ± 2.81b Chewiness 331.724 ± 4.45a 59.160 ± 3.45d 158.199 ± 2.45c 268.101 ± 4.45b Sample A: control durum wheat; Sample B: native Horse Chestnut flour; Sample C: 0.5% gum addition; Sample D: 1% gum addition. Results presented are mean values and the superscripts significantly differ in row (P < 0.05) (n = 6). Table 8. Sensory evaluation of pasta. Parameters Sample A Sample B Sample C Sample D Slipperiness 3.67a ± 0.82 1.83c ± 0.98 3.00b ± 1.10 3.53a ± 1.75 Firmness 4.00a ± 1.26 1.50c ± 0.84 2.50b ± 1.22 3.33a ± 0.82 Chewiness 3.50a ± 1.97 2.67b ± 1.52 3.00a ± 1.51 3.57a ± 1.26 Surface adhesiveness 3.50a ± 1.22 1.13c ± 1.03 2.50b ± 1.52 3.40a ± 1.02 Appearance 4.33a ± 0.82 2.67c ± 1.51 3.17b ± 1.60 4.00a ± 1.67 Overall sensory score 4.5a ± 0.26 1.63c ± 0.13 2.94b ± 0.10 4.43a ± 0.17 Sample A: control durum wheat; Sample B: native Horse Chestnut flour; Sample C: 0.5% gum addition; Sample D: 1% gum addition. Mean ± S.D. with different superscripts in a row differ significantly (P < 0.05) (n = 12). and gelatinization of starch. Besides, addition of gum results in the development of continuous protein net- work, which reduces pasta adhesiveness. Padalino et  al. (2013) and Widelska et al. (2019) also observed that the hydrocolloid addition to the gluten-free pasta formula- tion led to the decrease in adhesiveness. Other textural attributes including cohesiveness, springiness, gummi- ness, and chewiness also increased with the addition of gum in pasta formulation. Mirhosseini et al. (2015) observed a similar kind of improvement in textural attributes with the addition of polysaccharide gums to gluten-free pasta formulations. HCN-flour-based pasta made with 1 % gum show comparable textural properties with that of the control sample. Sensory quality of pasta The prepared pasta samples were evaluated for appear- ance, firmness, chewiness, surface adhesiveness, and slipperiness by sensory panellists using a 5-point scale. The mean values of sensory attributes for pasta samples are shown in Table 8. Sensory score of native HCN pasta (Sample B) was significantly low compared to control sample (sample A), which could be related to the dif- ference in composition and properties of their corre- sponding flours (Bolarinwa and Oyesiji, 2021). Among the HCN-flour-based pasta samples, Sample B (0% gum) showed a minimum score for sensory attributes (appear- ance, firmness, chewiness, slipperiness, and adhesive- ness) while sample D (1% gum) showed the highest score. No significant difference was observed in the sen- sory parameters of Sample A (control) and Sample D. Overall, sensory score of Sample D was comparable with that of the control sample. Enhancement in the textural properties of pasta with the addition of gums in pasta formulation led to an improvement in sensory qual- ity. Shere et al. (2020) also observed that gum addition enhanced the overall sensory quality of the developed instant noodles. Conclusions The results provide an insight about the physicochemical, functional, pasting, and thermal properties of HCN flour, and the quality characteristics of pasta made from HCN flour. The results showed significant difference between the various properties of wheat and HCN flour. Difference in the ratio of flour constituents (protein, starch, etc.) could be the reason for the difference in properties of the two flours. Cooking, textural, and sensory char- acteristics of HCN-flour-based pasta samples showed that good-quality pasta comparable with commercial 146 Italian Journal of Food Science, 2021; 33 (SP1) Rafiq SI et al. sensory attributes. Heliyon 7(1): e06052. https://doi.org/10.1016/j. heliyon.2021.e06052 Bresciani, A., Giordano, D., Vanara, F., Blandino, M. and Marti, A., 2021. High-amylose corn in gluten-free pasta: strategies to deliver nutritional benefits ensuring the overall quality. 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Italian Journal of Food Science 30(2): 226–248 Dick, J.W. and Youngs, V.L., 1988. Evaluation of durum wheat, sem- olina, and pasta in the United States. Available at: https://agris. fao.org/agris-search/search.do?recordID=US19890103346. Drakos, A., Kyriakakis, G., Evageliou, V., Protonotariou, S., Mandala,  I. and Ritzoulis, C., 2017. Influence of jet milling and particle size on the composition, physicochemical and mechan- ical properties of barley and rye flours. Food Chemistry 215: 326–332. https://doi.org/10.1016/j.foodchem.2016.07.169 Du, S.K., Jiang, H., Yu, X. and Jane, J.L., 2014. Physicochemical and functional properties of whole legume flour. LWT— Food Science and Technology 55(1): 308–313. https://doi. org/10.1016/j.lwt.2013.06.001 Duda, A., Adamczak, J., Chełmińska, P., Juszkiewicz, J. and Kowalczewski, P., 2019. Quality and nutritional/textural proper- ties of durum wheat pasta enriched with cricket powder. Foods 8(2): 46. https://doi.org/10.3390/foods8020046 wheat-based pasta could be prepared from HCN flour. The study would serve as a template about the derived properties of HCN flour. Furthermore, gluten-free pasta could be made from HCN flour using appropriate pro- portion of gum in the production process. In addition, the study suggests that HCN flour could be used as a substitute for conventional flours for the development of gluten-free pasta. Further research should be carried out to determine the possible utilization of HCN flour in the development of other food products. Acknowledgments The first author is thankful to the UGC for Maulana Azad National Fellowship. Conflict of Interest The authors state no conflict of interest. References AACC, 2000. Approved methods of the American Association of Cereal Chemistry, American Association of Cereal Chemists Inc., St. Paul, MN. Abu, J.O., Duodu, K.G. and Minnaar, A., 2006. 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