P U B L I C A T I O N S CODON Italian Journal of Food Science, 2023; 35 (3): 99–114 ISSN 1120-1770 online, DOI 10.15586/ijfs.v35i3.2302 99 P U B L I C A T I O N S CODON Quality and stability of different seafood products treated with high hydrostatic pressure (HPP) intended for raw consumption Ana Cristina de Aguiar Saldanha Pinheiro1, Silvia Tappi1,2*, Giacomo Braschi1,2, Jessica Genovese1, Francesca Patrignani1,2, Pietro Rocculi1,2 1Department of Agricultural and Food Science, Alma Mater Studiorum, University of Bologna, Campus of Food Science, Piazza Goidanich 60, Cesena (FC), Italy; 2Interdepartmental Centre for Agri-Food Industrial Research, Alma Mater Studiorum, University of Bologna, Campus of Food Science, Via Ravennate 933, Cesena (FC), Italy *Corresponding Author: Silvia Tappi, Department of Agricultural and Food Science, Alma Mater Studiorum, University of Bologna, Campus of Food Science, Piazza Goidanich 60, Cesena (FC), Italy Email: silvia.tappi2@unibo.it Received: 15 November 2022; Accepted: 23 June 2023; Published: 23 August 2023 © 2023 Codon Publications OPEN ACCESS PAPER Abstract The consumption of raw fish has rapidly increased in recent years, but being a highly perishable product, it is characterised by a very short microbiological shelf life. High hydrostatic pressure (HPP) processing is a non- thermal technology has emerged recently as a promising alternative to thermal processing for food pasteurization capable of maintaining fresh-like characteristics and nutritional value. However, the induced changes in product quality should be assessed carefully. The present research aimed to investigate the effect of HPP on different seafood products, namely grey mullet, tiger prawn and rose shrimp, intended for raw consumption. Three pres- sure levels (400, 500 and 600 MPa) were applied for 10 min. During refrigerated storage, microbiological quality, chemical parameters, colour and texture and fat oxidation were analysed. Results showed that the application of lower pressure was able to inactivate E. coli, pseudomonas and/or positive coagulase staphylococci; however, they were able to recover during storage. In addition, the application of 600-MPa pressure extended the microbio- logical shelf life by up to 30 days. For all samples, general whitening occurred while the texture was affected in a different way for the three considered species. Fat oxidation was only minimally affected and remained quite low during storage. Keywords: grey mullet, microbiological inactivation, rose shrimp, shelf life, tiger prawn Introduction The consumption of raw fish has rapidly increased in recent years, also in the areas where it was not a tradi- tional habit, because of not only changes in food taste but also to the adoption of culinary traditions of other coun- tries. Sushi and sashimi which are typically oriental spe- cialities are becoming increasingly popular in European countries as well. Moreover, the use of low-temperature cooking and processing, such as cold smoking, is spread- ing fast (Brutti et al., 2010). These new habits have increased microbiological risks for fish product consumption. Moreover, seafood prod- ucts are highly perishable, their microbiological shelf life is very short and, in order to sell a fish product to be consumed raw, a strategy to increase its shelf life could increase its marketability. Therefore, a non-thermal tech- nology able to reduce microbial load is highly necessary. High-pressure processing (HPP) is a non-thermal tech- nology that has recently emerged as a promising alter- native to thermal processing for food pasteurization mailto:silvia.tappi2@unibo.it 100 Italian Journal of Food Science, 2023; 35 (3) Pinheiro ACAS et al. European Union (EU) legislation requires seafood prod- ucts to be frozen for at least 24 h before raw consump- tion, HPP was applied to frozen-thawed products. Three pressure levels were applied, and microbiological quality, safety, chemical parameters, colour and texture were ana- lysed during the refrigerated storage. Materials and Methods Preparation of fish samples Grey mullet (Mugil cephalus) striped prawn (Melicertus kerathurus) and deep-water rose shrimp (Parapenaeus longirostris) were fished in the Adriatic Sea. Products were fast-frozen in an industrial blast chiller at a tem- perature of -18°C and kept for 24 h at Economia del Mare (Cesenatico, Italy). Thawing was carried out at 4°C for 16  h; then the seafood samples were subjected to mechanical deboning and shell removal. Flesh was manually cut into pieces and packaged in polyethylene terephthalate (PET) trays (VR11-6T-T1, Technopress Srl., FC, Italy) containing six mono-portions of 15–20 g approximately, each sealed under vacuum with a high barrier PET film (thickness 40 µm; oxygen transmission rate [OTR] 60 cm3/m2/24 h/bar; 4°C, 0% RH; Cryovac Sealed Air Corp., NJ, USA). HPP treatment The vacuum-packed samples were subjected to HPP treatment performed by HPP Italia s.r.l (Parma, Italy). The samples were placed in a 350-L chamber, filled with water and subjected to 400-, 500- or 600-MPa pressure for 10 min. An untreated sample was used as a control. The samples were coded using initial of the species (M for grey mullet, P for striped prawn and S for rose shrimp) and 0-, 400-, 500- and 600-MPa pressure was applied. For each treatment, 24 packages were prepared. Storage After treatment, the samples were stored in the labora- tories of the Campus of Food Science of the University of Bologna at 2 ± 1°C. During storage, the samples were subjected to analytical determinations after 0, 1, 6, 9, 14, 21, 28 and 35 days. Storage duration was determined for each sample based on the results of microbiological anal- ysis, considering the end of the shelf life when reaching a microbial load of 6 log cfu/g referred to total mesophilic bacteria. Three different packages were used for each HPP treat- ment at each storage period. capable of maintaining fresh-like characteristics and nutritional value. Application of pressure higher than 300 MPa for a few minutes at room temperature has shown to significantly reduce the initial microbial load in many fish species (Truong et al., 2015). The extent of microbial inactivation depends not only on treatment parameters, such as pressure level, holding time and temperature, but also on the characteristics of microflora in the product (Truong et al., 2015). The inactivation of microorganisms by HPP is the result of a combination of factors, includ- ing changes in cell membranes, cell walls, proteins and enzyme-mediated cellular functions (Campus, 2010). Cell membranes are the primary site of pressure-induced damage, with consequent alterations of cell permeability, transport systems, loss of osmotic responsiveness, organ- elle disruption and inability to maintain intracellular pH. In general, Gram-negative bacteria and cells in the growth phase are more sensitive than Gram-positive bacteria and cells in the stationary phase. Nevertheless, investigations have shown that cell disruption is more dependent on the geometry of the bacteria, rather than Gram-type. For example, morphological changes in the rod-shaped Escherichia coli and Pseudomonas aeru- ginosa were observed whereas Staphylococcus aureus (cocci) was more resistant to pressure. However, in complex matrices such as food, the desired effect on microbial inactivation may also produce physi- cal and biochemical changes, which may affect the prod- uct properties negatively. The denaturation of proteins in fish muscle could cause significant changes of important parameters for con- sumer acceptability. In particular, the application of high pressure is known to lead to a cooked appearance (Matser et al., 2000), which could be specifically detrimental in products intended for raw consumption. Moreover, the effect of protein structure on enzymatic activity can lead to variations in the textural properties of seafood prod- ucts not only after the treatment but also during refrig- erated storage. The effect of HPP has been studied in a variety of fish and seafood matrices, but results are very variable depending on process parameters as well as spe- cific product characteristics (Truong et al., 2015). In a product aimed for raw consumption, microbiological quality is of paramount importance throughout the shelf life; however, the effect of HPP on quality could lead to undesirable changes. The aim of the present research was to investigate the effect of HPP treatment on different types of seafood products, that is, grey mullet, tiger prawn and rose shrimp, intended for raw consumption. Because the Italian Journal of Food Science, 2023; 35 (3) 101 Effect of high hydrostatic pressure on different seafood products For each sample and storage time, an average of at least 15 measurements was calculated. Texture The texture was evaluated with a texture analyser model TA.HDi 500 (Stable Micro Systems, Godalming, UK) equipped with a 25-kg load cell. Briefly, 15 g of each sam- ple was inserted in a cylindrical cup (diameter of 3  cm) and a piston was used to compress the sample at 1 mm/s up to 50% of its height. The pressure was held for 60 s. Maximum force was considered as hardness (force, F1 [Newton, N]) and force at the end of the compression was considered as index of resistance to compression (force F2 [N]). The texture was evaluated with at least six replicates for each sample. Peroxide value (PV) Lipids were extracted from 25 g of sample with a method described by Bligh and Dyer (1959). The peroxide value was determined by the ferrothiocyanate method (Chapman and Mackay, 1949). Results were expressed as the amount of oxygen (O2) per kilogram of fat/oil (mEq O2/kg fat/oil). The analysis was performed with at least triplicates for each sample. Statistical analysis The significance of differences was tested by the analy- sis of variance (ANOVA) using Tukey’s honestly signif- icant difference (HSD) test as a post-hoc test (p < 0.05). Statistical analysis was conducted with Statistica 8.0 for Windows. Results and Discussion Microbial inactivation The effects of HPP treatments (400, 500 and 600 MPa), compared to the untreated controls, on the microbiolog- ical quality of packaged grey mullet, striped prawn and rose shrimp are reported in Tables 1, 2 and 3, respectively. In all tested conditions, Salmonella spp. and Listeria monocytogenes were not detected during the shelf life of the considered products. In addition, coagulase positive staphylococci were not found in untreated and HPP-treated samples of grey mullets and striped prawn. In general, the application of HPP treatments increased the microbiological shelf life of the considered products, and the inactivation effect became more severe with increased pressure. The micro- biological threshold to define product shelf life was fixed Analytical determinations Microbiological analyses Microbiological analyses were performed on untreated grey mullet, striped prawn and red shrimp, and the sam- ples were treated with 400-, 500- and 600-MPa pressure. Immediately after HPP treatments for the stated storage period, all the samples were investigated for the presence of Salmonella spp. and Listeria monocytogenes according to EN ISO 6579-1:2017/A1:2020 and ISO 11290-1:2017. Microbial groups considered in this research were total mesophilic bacteria (TMB), lactobacillus spp., pseudo- monas spp., sulphite-reducing anaerobic bacteria, total Coliforms, E. coli, and coagulase positive staphylococci. In all, 10 g of samples were serially diluted using phys- iological saline solution (0.9% NaCl), and appropriate inoculum was included or spread in different selective culture media, such as plate count agar (PCA; Oxoid- Thermofisher, Milan, Italy) for TMB; De Man, Rogosa and Sharpe agar (MRS; Oxoid-Thermofisher) sup- plemented with cycloheximide (0.2% p/v) for lacto- bacillus spp.; selective pseudomonas agar base (PAB; Oxoid-Thermofisher) for pseudomonas spp.; rein- forced clostridial agar (RCA; Oxoid-Thermofisher) for sulphite-reducing anaerobic bacteria, violet red bile agar (VRBA; Oxoid-Thermofisher) supplemented with 4-methylumbelliferyl-β-D-glucuronide (MUG; Oxoid-Thermofisher) for total coliforms and E. coli, respectively; and Baird Parker agar (BP) for coagulase positive staphylococci. Plates were incubated for 24/48 h at 30°C for pseudomonas spp. (PA) and 37°C for lac- tobacilli, sulphite-reducing anaerobic bacteria, total coliform, E. coli and coagulase positive staphylococci. Sulphite-reducing anaerobic bacteria were incubated under anaerobic conditions using a gas generating kit (Oxoid-Thermofisher). pH values pH values of the samples homogenised for 60 s with an Ultraturrax (T-25, Ika, Germany) with distilled water (sample–water ratio: 1:2 w/w) were assessed with a pH meter (Crison, Barcellona). The analysis was performed at least in triplicate for each sample. Colour Colour parameters lightness (L*), redness (a*) and yel- lowness (b*) were measured with a spectrophotocolorim- eter model ColorFlex™ (Hunterlab, Reston, VA, US). The tristimulus L*, a*, b* measurement mode (International Commission on Illumination [CIE], 1976) was used. The hue angle (h°) was calculated as follows: 1 btan ah 360 2π ∗ − ∗ = ×� 102 Italian Journal of Food Science, 2023; 35 (3) Pinheiro ACAS et al. Table 1. Evolution of microbial cell loads (log CFU/g) of total mesophilic count (TMC), lactobacillus spp., pseudomonas spp., total coliforms, sulphite-reducing anaerobic bacteria (AB), and E. coli during the refrigerated storage of packaged grey mullet (Mugil cephalus) flesh with applied high hydrostatic pressure (HPP) treatments (400, 500 and 600 MPa).     Mullet Log CFU/g M-0 M-400 M-500 M-600 Days of storage 0 d TMC 4.67 ± 0.43 <1** <1 <1 Lactobacillus spp. 3.18 ± 0.33 <1 <1 <1 Sulphite-reducing AB 4.20 ± 0.54 <1 <1 <1 Pseudomonas spp. 4.45 ± 0.22 <1 <1 <1 Total coliforms 2.56 ± 0.38 <1 <1 <1 E. coli 1.12 ± 0.10 <1 <1 <1 2 d TMC 5.14 ± 0.44 <1 <1 <1 Lactobacillus spp. 3.30 ± 0.29 <1 <1 <1 Sulphite-educing AB 5.15 ± 0.51 <1 <1 <1 Pseudomonas spp. 5.20 ± 0.45 <1 <1 <1 Total coliforms 1.80 ± 0.12 <1 <1 <1 E. coli  1.22 ± 0.10  <1 <1 <1 6 d TMC 6.22 ± 0.52 <1 <1 <1 Lactobacillus spp. 4.00 ± 0.28 <1 <1 <1 Sulphite-reducing AB 5.54 ± 0.46 <1 <1 <1 Pseudomonas spp. 5.27 ± 0.35 <1 <1 <1 Total coliforms 1.85 ± 0.16 <1 <1 <1 E. coli 1.50 ± 0.20 <1 <1 <1 12 d TMC -* 5.67 ± 0.31 <1 <1 Lactobacillus spp. - <1 <1 <1 Sulphite-reducing AB - <1 <1 <1 Pseudomonas spp. - 2.46 ± 0.29 <1 <1 Total coliforms - <1 <1 <1 E. coli    <1 <1 <1 19 d TMC - 7.52a ± 0.43 4.22b ± 0.41 2.20c ± 0.23 Lactobacillus spp. - 1.06 ± 0.32 <1 <1 Sulphite-reducing AB - <1 <1 <1 Pseudomonas spp. - 5.11 ± 0.29 <1 <1 Total coliforms - <1 <1 <1 E. coli   <1 <1 <1 32 d TMC - - 5.55a ± 0.42 3.10b ± 0.33 Lactobacillus spp. - - <1 <1 Sulphite-reducing AB - - <1 <1 Pseudomonas spp. - - <1 <1 Total coliforms - - <1 <1 E. coli   - <1 <1 *Not analysed because the microbiological threshold fixed at 6 log CFU/g was reached in the previous time of analysis. **Under detection limit. In the same row, values with different superscript letters are significantly different (p < 0.05). Italian Journal of Food Science, 2023; 35 (3) 103 Effect of high hydrostatic pressure on different seafood products as an increased yield of the shucking process of bivalves and crustaceans (Patterson, 2014). In general, according to the literature, HPP between 150 MPa and 450 MPa is commonly applied on fish samples (Perez-Won et al., 2020) because higher pressure, aimed to increase micro- bial inactivation, is generally associated with significant changes in physicochemical, texture and sensory proper- ties of products, such as increase in discoloration, cooked appearance or lipid oxidation (Truong et al., 2015). However, the data resulting from this research evidenced that HPP treatment at 400 MPa is not able to increase significantly the shelf life of the considered products. Although this level of pressure seemed adequate to inac- tivate E. coli during the shelf life of the considered fish samples, other microbial groups, involved in spoilage and safety issue, such as pseudomonas or positive coag- ulase staphylococci, were able to retrieve during storage, highlighting the critical issue of viable but not culturable (VBNC) cells. Although the efficiency of microbial inac- tivation is influenced by various factors, such as food matrix characteristics and food processing parameters, the physiological diversity within a microbial population also has to be taken into consideration, especially in the validation of the effectiveness of a treatment on a spe- cific food product (Patrignani et al., 2019). Moreover, a further consideration is required in relation to the bio- genic amines, potentially present in these types of prod- ucts. In fact, although histamine was not detected, the application of 400–600-MPa HPP appears to reduce sev- eral microbial groups that are able to produce biogenic amines, such as lactic acid bacteria and coliforms, reduc- ing the risk of production of these indicators of food safety and quality indices (Borges et al. 2023). Pseudomonas spp., strict aerobic bacteria, whose growth decreased in vacuum, was able to recover in grey mul- let and striped prawn samples treated at 400 MPa during their shelf life. Pseudomonas spp., also having a psy- chrotrophic behaviour and able to produce specific H2S off-flavours, could have a negative impact to produce specific proteases, which could potentially affect the tex- tural properties of food matrix. pH value The initial pH values measured in grey mullet, tiger prawn and rose shrimp samples according to HPP treat- ment and during storage are reported in Table 4. The initial values for the untreated samples were 6.4 ± 0.1, 7.2 ± 0.01, 7.6 ± 0.03 for grey mullet, tiger prawn and rose shrimp, respectively. While pH values for grey mullet were consistent with the literature (Tsogas et al., 2019), for prawn and shrimp, these values were slightly higher, compared to literature (Bindu et al., 2013; Kaur et al. 2013). at the attaining of 6 log CFU/g for total mesophilic count (TMC) even if, according to Regulation 2073/2005, other important microbiological criteria, such as cell load of Escherichia coli and positive coagulase staphylococci, were considered in the data discussion. More specifically, the detected microbiological data for grey mullets, untreated and in relation to the pressure applied and storage period are reported in Table 1. As shown, the control sample was spoiled within 6 days of storage at 2°C, reaching a cell load of total mesophilic bacteria of 6.22 log CFU/g with a corresponding level of total coliforms of 1.8 log CFU/g. In contrast, application of 400, 500 and 600 MPa pressure prolonged product shelf life to 12, 19 and 32 days, respectively. The application of HPP treatments ranging between 400 MPa and 600 MPa decreased the cell loads of E. coli, compared to the untreated sample, under the detection limit of 1 log CFU/g. Pseudomonas spp. was strongly affected by the level of applied HPP since cell load of 2.4 CFU/g were detected at 400-MPa pressure after 12 days of treatment to fur- ther increase on the 19th day of storage. In all the other treated samples, and for each time of sampling consid- ered, Pseudomonas spp. was always under the detection limit (<1 Log CFU/g). Regarding untreated striped prawn (Table 2), the total mesophilic bacteria reached the threshold level of 6.0 log CFU/g after 6 days of refrigerated storage, while the application of HPP pressure of 500 MPa and 600 MPa determined a significant increase in the product’s shelf life to 19 days and 32 days, respectively, and the cell load of E. coli was always under the detection limit. However, for the sample treated at 500-MPa pressure, after 19 days, lactobacilli and pseudomonas spp. were able to recover and reach the respective cell load of 4.49 log CFU/g and 5.72 log CFU/g, different from the samples treated at 600 MPa, where these microbial groups were found to be under the detection limit. Applying a pressure level of 400 MPa resulted in a shelf life of about 12 days. Similar trends were observed in the shrimp products (Table 3), where the application of a pressure of 600 MPa allowed the shelf-life threshold to reach after 28 days. In contrast, the samples treated with 500-MPa pressure reached the spoilage threshold after 21 days. Interestingly, 400-MPa pressure was not able to inactivate completely the coagulase-positive staphylo- cocci, which were able to recover but only after reaching the shelf-life threshold. The rationale for the use of HPP for fish and fish prod- ucts is based on its ability to inactivate pathogenic and spoilage microorganisms and microbial enzymes, result- ing in an increased shelf life (de Alba et al., 2019) as well 104 Italian Journal of Food Science, 2023; 35 (3) Pinheiro ACAS et al. Table 2. Evolution of microbial cell loads (log CFU/g) of total mesophilic count (TMC), lactobacillus spp., pseudomonas spp., total coliforms, sulphite-reducing anaerobic bacteria (AB), and E. coli during the refrigerated storage of packaged striped prawn with applied high hydrostatic pressure (HPP) treatments (400, 500 and 600 MPa). Striped prawn Log CFU/g P-0 P-400 P-500 P-600 Days of storage 0 d TMC 4.65a ± 0.44 2.45b ± 0.43 <1 <1 Lactobacillus spp. 3.95 ± 0.29 <1** <1 <1 Sulphite-reducing AB 3.83 ± 0.39 <1 <1 <1 Pseudomonas spp. 4.63 ± 0.56 <1 <1 <1 Total coliforms 4.60a ± 0.48 1.30b ± 0.33 <1 <1 E. coli 2.10 ± 0.33 <1 <1 <1 2 d TMC 4.50a ± 0.46 2.96b ± 0.43 <1 <1 Lactobacillus spp. 3.50 ± 0.36 <1 <1 <1 Sulphite-reducing AB 3.20 ± 0.51 <1 <1 <1 Pseudomonas spp. 5.25 ± 0.48 <1 <1 <1 Total coliforms 4.75 ± 0.49 <1 <1 <1 E. coli 2.80 ± 0.15 <1 <1 <1 6 d TMC 6.30a ± 0.52 2.88b ± 0.55 <1 <1 Lactobacillus spp. 5.82 ± 0.42 <1 <1 <1 Sulphite reducing AB 4.42 ± 0.49 <1 <1 <1 Pseudomonas spp. 5.35 ± 0.35 <1 <1 <1 Total coliforms 3.75 ± 0.46 <1 <1 <1 E. coli 2.95 ± 0.15 <1 <1 <1 12 d TMC 7.20a ± 0.39 4.61b ± 0.23 2.00c ± 0.15 1.50c ± 0.50 Lactobacillus spp. 6.80a ± 0.55 5.12b ± 0.34 <1 <1 Sulphite reducing AB 5.84 ± 0.51 <1 <1 <1 Pseudomonas spp. 5.30a ± 0.45 3.88b ± 0.39 <1 <1 Total coliforms 3.72a ± 0.43 1.48b ± 0.46 <1 <1 E. coli 3.20 ± 0.23 <1 <1 <1 19 d TMC - 8.35a ± 0.48 6.18b ± 0.35 4.80c ± 0.39 Lactobacillus spp. - 6.56a ± 0.30 4.59b ± 0.33 <1 Sulphite-reducing AB - <1 <1 <1 Pseudomonas spp. - 7.83a ± 0.37 5.72b ± 0.47 <1 Total coliforms - 1.95 ± 0.34 <1 <1 E. coli <1 <1 <1 32 d TMC - - - 6.51 ± 0.39 Lactobacillus spp. - - - <1 Sulphite-reducing AB - - - <1 Pseudomonas spp. - - - <1 Total coliforms - - - <1 E. coli <1 *Not analysed because the microbiological threshold fixed at 6 log CFU/g was reached in the previous time of analysis. **Under detection limit. In the same row, values with different superscript letters are significantly different (p < 0.05). Italian Journal of Food Science, 2023; 35 (3) 105 Effect of high hydrostatic pressure on different seafood products Table 3. Evolution of microbial cell loads (log CFU/g) of total mesophilic count (TMC), lactobacillus spp., pseudomonas spp., total coliforms, sulphite-reducing anaerobic bacteria (AB), E. coli and positive coagulase (PC) staphilococci during the refrigerated storage of packaged rose shrimp in relation to the applied high hydrostatic pressure (HPP) treatments (400, 500 and 600 MPa).   Rose shrimp   Log CFU/g S-0 S-400 S-500 S-600 Days of storage 0 d TMC 5.04a ± 0.47 4.46a,b ± 0.56 3.78b,c ± 0.53 3.34c ± 0.45 Lactobacillus spp. 5.34 ± 0.44 <1** <1 <1 Sulphite-reducing AB 4.53a ± 0.51 2.48b ± 0.35 2.38b ± 0.43 <1 Pseudomonas spp. 5.16 ± 0.37 <1 <1 <1 Total Coliforms 3.53 ± 0.46 <1 <1 <1 E. coli 2.26 ± 0.45 <1 <1 <1 PC staphilococci 2.20 ± 0.20 <2 <2 <2 7 d TMC 6.48a ± 0.49 5.18b ± 0.46 4.35b,c ± 0.39 3.63c ± 0.47 Lactobacillus spp. 6.05 ± 0.55 <1 <1 <2 Sulphite-reducing AB 6.08 ± 0.36 <2 <2 <1 Pseudomonas spp. 5.32 ± 0.42 <1 <1 <1 Total coliforms  4.70 ± 0.20 <1 <1 <1 E. coli  3.60 ± 0.15 <1 <1 <1 PC staphilococci 3.40 ± 0.25 <2 <2 <2 14 d TMC -* 8.15a ± 0.35 4.31b ± 0.51 4.20b ± 0.56 Lactobacillus spp. - <2 <1 <1 Sulphite-reducing AB - <2 <2 <1 Pseudomonas spp. - 3.56 ± 0.44 <1 <1 Total coliforms - <1 <1 <1 E. coli   <1 <1 <1 PC staphilococci   2.60 ± 0.41 <2 <2 21 d TMC - - 5.64 ± 0.42 3.6 ± 0.41 Lactobacillus spp. - - <1 <1 Sulphite-reducing AB - - <2 <1 Pseudomonas spp. - - <1 <1 Total coliforms - - <1 <1 E. coli   - <1 <1 PC staphilococci   - <2 <2 28 d TMC - - 8.43a ± 0.37 5.46b ± 0.43 Lactobacillus spp. - - <1 <1 Sulphite-reducing AB - - 4.32 ± 0.33 <1 Pseudomonas spp. - - <1 <1 Total coliforms - - <1 <1 E. coli   - <1 <1 PC staphilococci   - <2 <2 35 d TMC - - - 7.49 ± 0.39 Lactobacillus spp. - - - <1 Sulphite-reducing AB - - - <1 Pseudomonas spp. - - - <1 Total coliforms - - - <1 E. coli - - - <1 PC staphilococci - - - <2 *Not analysed because the microbiological threshold fixed at 6 log CFU/g was reached in the previous time of analysis **Under detection limit. In the same row, values with different superscript letters are significantly different (p < 0.05). 106 Italian Journal of Food Science, 2023; 35 (3) Pinheiro ACAS et al. Table 4. pH and peroxide values (PV; mEq O 2 /kg fat) measured in HPP-treated seafood samples during refrigerated storage. Storage time (days) 1 6 9 14 21 28 35 Sample pH M-0 6.41 ± 0.13a 6.34 ± 0.03b 6.46 ± 0.05a 6.23 ± 0.03b M-400 6.44 ± 0.06a 6.55 ± 0.06a 6.52 ± 0.04a 6.47 ± 0.02a 6.34 ± 0.07c M-500 6.44 ± 0.01a 6.50 ± 0.04a 6.45 ± 0.01a 6.46 ± 0.01a 6.51 ± 0.04a 6.31 ± 0.10a 6.51 ± 0.01a M-600 6.46 ± 0.01a 6.50 ± 0.03a 6.41 ± 0.02a 6.45 ± 0.01a 6.41 ± 0.03b 6.40 ± 0.03a 6.51 ± 0.01a P-0 7.18 ± 0.01b 7.20± 0.01c 7.54 ± 0.12a 7.44 ± 0.06b P-400 7.45 ± 0.01a 7.29 ± 0.03b 7.27 ± 0.01b 7.25 ± 0.01c 7.06 ± 0.01c P-500 7.40 ± 0.01a 7.45 ± 0.01a 7.32 ± 0.01b 7.24 ± 0.03c 7.47 ± 0.02a 7.27 ± 0.04b 7.17 ± 0.01b P-600 7.50 ± 0.02a 7.44 ± 0.08a 7.26 ± 0.02b 7.54 ± 0.02a 7.36 ± 0.03b 7.36 ± 0.01a 7.37 ± 0.01a S-0 7.59 ± 0.04b 7.68 ± 0.08a 7.73 ± 0.03a S-400 7.65 ± 0.05a 7.44 ± 0.03b 7.47 ± 0.03b 7.46 ± 0.01a S-500 7.55 ± 0.11b 7.52 ± 0.02b 7.50 ± 0.01b 7.39 ± 0.03a 7.37 ± 0.01b S-600 7.74 ± 0.02a 7.61 ± 0.01ab 7.48 ± 0.01b 7.51 ± 0.04a 7.48 ± 0.01a 7.41 ± 0.02 Sample PV M-0 0.88 ± 0.09b 0.55 ± 0.08b 1.40 ± 0.05a 0.33 ± 0.10b M-400 1.00 ± 0.06b 0.68 ± 0.10b 0.77 ± 0.06b 1.50 ± 0.11a 1.52 ± 0.07a M-500 0.99 ± 0.05b 0.90 ± 0.14a 1.00 ± 0.22ab 1.25 ± 0.11a 1.10 ± 0.06a 1.51 ± 0.14a 0.79 ± 0.04b M-600 1.28 ± 0.05a 0.92 ± 0.07a 0.92 ± 0.05b 1.14 ± 0.05a 1.57 ± 0.10a 1.06 ± 0.17a 1.75 ± 0.15a P-0 1.88 ± 0.03ab 1.36 ± 0.15b 1.56 ± 0.16b P-400 2.03 ± 0.02a 2.03 ± 0.11a 1.37 ± 0.02c 1.30 ± 0.14a 1.30 ± 0.14a P-500 1.54 ± 0.14b 2.5 ± 0.07a 2.68 ± 0.41a 1.57 ± 0.11a 1.92 ± 0.31a 1.89 ± 0.07a 1.78 ± 0.2a P-600 1.52 ± 0.11b 0.70 ± 0.07c 1.96 ± 0.11b 1.44 ± 0.16a 1.82 ± 0.12a 0.89 ± 0.03b 1.66 ± 0.12a S-0 0.91 ± 0.14a 0.43 ± 0.02b 0.59 ± 0.01b S-400 0.63 ± 0.12a 0.82 ± 0.13a 0.59 ± 0.02b 0.68 ± 0.11a S-500 0.66 ± 0.08a 0.86 ± 0.04a 0.82 ± 0.05a 0.72 ± 0.01a 0.62 ± 0.04a S-600 0.98 ± 0.10a 0.67 ± 0.03ab 0.85 ± 0.08a 0.57 ± 0.13a 0.49 ± 0.02a 0.65 ± 0.04 Different superscript letters indicate significant differences (p < 0.05) between samples of the same specie at the same storage time. In the present study, despite no difference being observed between grey mullet samples just after treat- ment, after 6 days, pH was slightly higher in HPP- treated samples, compared to the untreated sample. During storage, a slight decrease was observed for M-0 sample, while for the samples subjected to high pres- sure, values showed little variability until the end of the storage period. On the other hand, for tiger prawn, all samples showed higher pH values of about 7.2–7.5 after HPP, compared to the control, without differences in relation to differ- ent pressure levels applied. However, while in the case of control sample, an increase in pH was observed during storage, treated samples showed an opposite trend (Figure S1). In sample P-400, pH values decreased by about 0.4 points, while in samples P-500 and P-600, they showed little variability. In rose shrimp, only sam- ple S-600 showed a significant increase in pH just after treatment, compared to the untreated sample. During storage, the control sample showed a slight increase, while for all treated samples, pH values decreased progressively. Increase in pH after pressurization of fish and seafood tissues was observed in literature (Angsupanich and Ledward, 1998; Bindu et al., 2013; Briones-Labarca et al., 2012; Kaur et al., 2013) and was explained with the induc- tion of protein unfolding by pressure and the following ionisation of denatured proteins. Italian Journal of Food Science, 2023; 35 (3) 107 Effect of high hydrostatic pressure on different seafood products Significant differences were observed for h° values between the control and the pressure-treated samples just after treatment. However, while for grey mullet, h° value increased due to HPP, for shrimp and prawn, the values of this chromatic parameter decreased. Hue angle is calculated using both red and yellow indexes; in all samples a* was decreased remarkably, while changes in b* were higher in shrimp and prawn, compared to grey mullet. According to the literature, variation in colour during storage could depend on degradation of myo- fibrillar proteins and disorganisation of myofibrils caused by enzymatic and non-enzymatic reactions as well as on the possible oxidation of pigments (Yagiz et al., 2007). Hence, change in colour occurring during storage in sea- food products subjected to high pressures could differ significantly depending on the species and the adopted treatment conditions. In the present study, the final effect for all samples was general whitening and occurrence of a cooked appearance, which is typical for muscle foods subjected to pressurization (Figure S2). Considering that visual quality is a very important parameter for consumer acceptability, and that these products are intended for raw consumption, the cooked On the other side, the evolution of pH during storage could be attributed mainly to the activity of spoilage microorganisms that produced both basic (e.g. ammo- nia, trimethylamine and other biogenic amines) and acidic (e.g. lactic acid in the case of lactobacillus spp.) compounds. Color Values for luminosity (L*) and hue angle (h°) of the three seafood species are reported in Figure 1. Luminosity of the flesh was significantly increased by about 20 units straight for the three considered species after HPP treat- ment. This effect was largely observed in many fish spe- cies and attributed to protein denaturation. Considering that for each investigated species there was no significant difference in the values of the treated products just after the treatment as a function of the different pressure lev- els adopted, it was assumed that protein denaturation occurred to a similar extent in all samples for HPP of 400–600 MPa. During storage, all L* values were very close to the initial values for the control and the pres- sure-treated samples. 30 40 50 60 70 80 30 40 50 60 70 80 30 40 50 60 70 80 90 0 10 20 30 40 50 60 70 20 25 30 35 40 45 50 55 60 60 65 70 75 80 85 90 95 0 5 10 15 20 25 30 35 0 5 10 15 20 25 30 35 0 5 10 15 20 25 30 35 ho L* ho L* L* L* ho h o Time (d) Time (d) Time (d) M-0 M-400 M-500 M-600 P-0 P-400 P-500 P-600 S0 S4 S5 S6 (A) (B) (C) (D) (E) (F) Figure 1. Colour coordinates of luminosity (L*) and hue angle (h°) of (A and B) mullet, (C and D) shrimp and (E and F) rose shrimp. Different letters indicate significant differences between samples (p < 0.05). 108 Italian Journal of Food Science, 2023; 35 (3) Pinheiro ACAS et al. influenced both myofibrillar proteins and connective tis- sues, bringing about myofibrillar fragility and gaping. A similar behaviour could be observed for sample M-400, but with a more gradual decrease of both values. Indeed, after 9 days, the resistance to compression was signifi- cantly higher, compared to the control, possibly because of a partial enzymatic denaturation caused by pressure. However, samples M-500 and M-600 showed quite con- stant values for both parameters for all storage periods with fewer differences between them. In striped prawn (Figures 2C and 2D), a slight but sig- nificant reduction of hardness was observed for the 500- and 600-MPa treatments, while the resistance parameter was reduced only for 600-MPa pressure. During storage, hardness showed a fluctuating behaviour but tended to increase, compared to the initial value for samples P-0, P-400 and P-500, while it remained practically unchanged for sample P-600. Resistance was shown to increase during storage only in samples P-0 and P-400, although the storage was shorter due to microbiological spoilage. On the contrary, in rose shrimp (Figures 2E and 2F), hardness was not influenced by any pressure applied, while the resistance to compression was found signifi- cantly higher after 600-MPa treatment. During storage, both parameters showed a decreasing trend for all sam- ples. The values were highly variable and very few signifi- cant changes were observed. appearance could represent a problem that could be addressed probably by a marketing and/or informative strategy. Texture Texture is a very important parameter for appreciation of seafood products. Even if, in general, the texture pro- file analysis (TPA) test is carried out on fish fillets, con- sidering the specific characteristics of the investigated product, in the present study compression, followed by the application of steady pressure, was applied. Results of the two parameters analysed, that are, hardness (F1) and index of resistance to compression (F2) (Figure S3), are reported in Figure 2 for all the three considered species. In the present work, the observed effect was different for the three considered species. For grey mullet (Figures 2A and 2B), a reduction in the initial hardness was observed for higher applied pressures (500 and 600 MPa) just after the treatment. Moreover, the increasing pressure decreased resistance to compression of the tissues in proportion to the applied pressure level (Figure 2B). For both parameters, in the control sample, a decrease was observed during stor- age, as reported by Chéret et al. (2005) for sea bass. The softening of the tissue during refrigerated storage could be attributed to enzymatic activity of proteases that 0 20 40 60 80 100 0 10 20 30 40 50 60 0 20 40 60 80 100 120 0 5 10 15 20 25 30 35 40 0 5 10 15 20 25 30 0 10 20 30 40 50 60 70 80 90 100 R es is ta nc e to c om pr es si on ( N ) R es is ta nc e to c om pr es si on ( N ) R es is ta nc e to c om pr es si on ( N ) H ar dn es s (N ) H ar dn es s (N ) H ar dn es s (N ) Time (d) Time (d) Time (d) 0 5 10 15 20 25 30 35 0 5 10 15 20 25 30 35 0 5 10 15 20 25 30 35 M-0 M-400 M-500 M-600 (A) (B) (C) (D) (E) (F) P-0 P-400 P-500 P-600 S0 S4 S5 S6 Figure 2. Texture parameters of hardness (N) and resistance to compression (N) of (A and B) mullet, (C and D) shrimp and (E and F) rose shrimp. Different letters indicate significant differences between samples (p < 0.05). Italian Journal of Food Science, 2023; 35 (3) 109 Effect of high hydrostatic pressure on different seafood products Low level of pressure of about 100 MPa applied at 10°C for 5 min showed to increase enzymatic activity of cal- pain in sea bass muscles (Chéret et al., 2005), while Teixeira et al. (2013) observed that rate of pressurization could also play a role in the activation of the same enzyme in sea bass fillets. In general, increase in pressure level and holding period was shown to inactivate enzymes. However, although seafood muscles were shown to be more sensitive to pressure compared to bovine muscles, the food matrix could strongly affect enzymes (Truong et al., 2015). The application of pressure caused variation in protein structure that could lead to the breakdown of cell membrane and release of proteolytic enzymes in the cytoplasm, favouring their denaturation. Lipid oxidation Table 4 shows peroxide values measured in the three considered species subjected to HPP treatment during refrigerated storage. A slight but significant increase in peroxide values was observed after 600-MPa treat- ment in grey mullet samples. However, while no differ- ence was observed in rose shrimp samples, lower values were measured in prawn samples treated at higher pres- sures. Moreover, peroxide values remained very low for all the storage periods considered (below 1.75, 2.7 and 0.98 mEq O2/kg fat for grey mullet, prawn and shrimp, respectively). In general, an enhancement of lipid oxidation was observed in the literature for many seafood products, particularly when pressures above 300 MPa were applied (Truong et al., 2015). This increase was mainly attributed to the presence of haemoglobin and myoglobin contain- ing iron in their structure that was released because of pressurization, and could promote oxidation. However, Yagiz et al. (2009) established that 300-MPa treatment helped to inhibit lipid oxidation of Atlantic salmon, com- pared to the untreated sample and the sample subjected to 150-MPa pressure during storage. The authors sug- gested that perturbation to changes to cell membrane structure caused by pressure made phospholipids less susceptible to oxidation even in a fish that was consid- ered fatty. Moreover, presence of astaxanthin (a carot- enoid) was believed to have a powerful antioxidant effect. Studies related to the effect of pressure on the activity of enzymes responsible for lipid oxidation are still scarce. The reduced oxidation level showed by the evolution of its primary index in the present study was due to dif- ferent reasons. Firstly, the considered seafood species were characterised by a low content of fat (about 2.5, 0.8 and 1.1 g/100 g for grey mullet, prawn and shrimp, respectively) and were not susceptible to lipid oxidation. Secondly, we assumed that further reduction in lipid These results are in contrast with increase in hardness measured after applying high pressure, as observed by Bindu et al. (2013) in Indian white prawn and by Jantakoson et al. (2012) and Kaur et al. (2013) in black tiger shrimp. Moreover, the two considered parameters between two crustaceans showed different behaviour during storage. An increasing tendency was observed in prawn, while in shrimp, values decreased progressively for the considered period. Decrease in hardness during storage, attributed to the effect of proteolytic enzymes, was also observed by Kaur et al. (2013). However, values of hardness in sample P-600 and resistance in samples P-500 and P-600 were mostly constant. This effect could be explained by a partial inactivation of such enzymes. In general, an increase in hardness after HPP treatment was observed by many authors on different fish species, such as Rainbow trout and Mahi mahi (Yagiz et al., 2007), cod (Angsupanich and Ledward, 1998), tuna (Zare, 2004). This was explained by the unfolding of actin and sarco- plasmic proteins and the formation of new hydrogen- bonded networks and by an increase in protein– protein interactions and bond formation. On the other hand, Briones-Labarca et al. (2012) found no differences in red abalone treated with up to 500-MPa HPP, compared to the control. Chéret et al. (2005) observed a softening effect after subjecting sea bass to a pressure of up to 300 MPa, while no change in hardness was observed with 400- and 500-MPa treatment. Besides modification to myofibrillar proteins, HPP also promoted pH values and modification to hydrogen and hydrophobic bonds that resulted in changes to the struc- tural characteristics of proteins. Moreover, an effect on collagen and the connective tissue of red abalone was observed by Briones-Labarca et al. (2012) through scan- ning electric microscope. This confirmed a significant change in the microstructure of the flesh upon applica- tion of high pressure. Hence, the effect of HPP on fish texture was due to modifications to water bonding and holding capacity, activity of enzymes, such as proteases, that could be inhibited or enhanced, and structural mod- ification of myofibrillar and sarcoplasmic protein. The results obtained in the present study confirmed that the effect on texture strictly depended on the considered species of the raw material and the specific tissue struc- ture. Moreover, the effect observed during storage prob- ably depended on the possible inactivation of proteolytic enzymes, which again probably was matrix-dependent. The effect of HPP on proteolytic enzymes in different species of fishes was studied by different authors, but results appeared to vary depending on seafood species, pressure level, holding period, and type and structure of the enzyme. 110 Italian Journal of Food Science, 2023; 35 (3) Pinheiro ACAS et al. environments as well as high mortality rate. In addition, the spore-forming quality of C. botulinum fosters its sur- vival in water sediments and fish, certainly influenced by geographical location, feeding habits of the fish species, types of product samples, and the detection method used (Aleksandr et al., 2015). Then again, the data empha- sized, following the HPP treatment, a shift in microbial populations responsible for reaching the threshold level. In addition, this aspect requires further investigation to understand product spoilage patterns. Author Contributions Conceptualization: S.T; P.R.; Methodology: S.T., F.P., P.R.; Formal analysis: A.C.D.A.S.P., G.B.; Investigation: A.C.D.A.S.P., G.B.; Resources: F.P., P.R.; Data cura- tion: A.C.D.A.S.P., J.G., G.B.; Writing-Original Draft: A.C.D.A.S.P., G.B.; Writing - Review & Editing: S.T., J.G., F.P., P.R.; Supervision: S.T, F.P.; Project Administration: P.R.; Funding Acquisition: F.P., P.R. References Aleksandr N., Margarita T., Inga E., Olga V., Vadims B. and Aivars B. 2015. Major food borne pathogens in fish and fish product: a review.  Ann Microbiol.  66(1): 1–15. https://doi.org/10.1007/ s13213-015-1102-5 Angsupanich K. and Ledward D.A. 1998. High pressure treatment effects on cod (Gadus Morhua) muscle. Food Chem. 63(1): 39–50. https://doi.org/10.1016/S0308-8146(97)00234-3 Bindu J., Ginson J., Kamalakanth C.K., Asha K.K and Srinivasa Gopal T.K. 2013. Physico-chemical changes in high pressure treated indian white prawn (Fenneropenaeus indicus) during chill storage. Innov Food Sci Emerg Technol. 17: 37–42. https:// doi.org/10.1016/j.ifset.2012.10.003 Bligh E.G. and Dyer W.J. 1959. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 37(8): 911– 17. https://doi.org/10.1139/o59-099 Borges A.F., Cózar A., Patarata L., Gama L.T., Alfaia C.M., Fernandes  M.J., et al. 2020s. Effect of high hydrostatic pres- sure challenge on biogenic amines, microbiota, and sensory profile in traditional poultry- and pork-based semidried fer- mented sausage.  J Food Sci.  85(4): 1256–1264. https://doi. org/10.1111/1750-3841.15101 Briones-Labarca V., Perez-Won M., Zamarca M., Aguilera- Radic  J.M. and Tabilo-Munizaga G. 2012. Effects of high hydrostatic pressure on microstructure, texture, colour and bio- chemical changes of red abalone (Haliotis rufecens) during cold storage time. Innov Food Sci Emerg Technol. 13: 42–50. https:// doi.org/10.1016/j.ifset.2011.09.002 Brutti A., Rovere P., Cavallero S., D’Amelio S., Danesi P. and Arcangeli G. 2010. Inactivation of anisakis simplex larvae in raw fish using high hydrostatic pressure treatments. Food Cont. 21(3): 331–333. https://doi.org/10.1016/j.foodcont.2009.05.013 oxidation could have been due to modification to cell membranes induced by pressure. Also, a very low pres- ence of oxygen in packages would have surely inhibited oxidative reactions during storage. Conclusions The investigation of the effect of HPP treatment on dif- ferent types of considered seafood products, intended for raw consumption, highlighted a significant increase in microbiological shelf life at the highest applied pressure levels (500 and 600 MPa) for all the considered species. Even if lower pressure (400 MPa) appeared adequate to inactivate E. coli, pseudomonas, and/or positive coag- ulase staphylococci, they were recovered during fish product storage, stressing the issue of viable but non- culturable cells (VBNC). On the other side, the applica- tion of 600  MPa allowed to extend the microbiological shelf life by up to around 30 days. In terms of visual quality, the final effect for all sam- ples was general whitening and occurrence of a cooked appearance, typical for muscle foods subjected to pres- surization. The texture response was strictly dependent on the considered species of raw material and specific tis- sue structure. Fat oxidation was minimally affected and remained quite low during storage. Further studies are in progress in our laboratories to bet- ter clarify the physico-chemical and biological causes of detected differences through microstructural assessment and their overall sensorial impact. Moreover, the effect of HPP on the production of biogenic amines is also under evaluation in order to clarify the effectiveness of the pro- posed technology on the safety aspect. However, considering the effect on microbiological shelf life, for all the three considered species, attaining a pressure of over 500 MPa appears necessary, particularly because the effect on quality did not show changes at higher pres- sures. It is important to emphasize that in the optic of the commercialisation of HPP-treated seafood intended for raw consumption, a specific marketing/informative strategy is required, evidencing to the final consumer the important advantages of this non-thermal technology in terms of nutritional and sensorial food properties. Moreover, in addition to L. monocytogenes and Salmonella pathogens, the effect of HPP process must be considered against Vibrio spp. and Clostridium bot- ulinum, especially in vacuum packaging, because these pathogens are responsible for human diseases through the consumption of contaminated fish and fish prod- ucts. 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Supplementary Italian Journal of Food Science, 2023; 35 (3) 113 Effect of high hydrostatic pressure on different seafood products M - 0M - 0 P - 0P - 0 S - 0S - 0 M - 400M - 400 P - 400P - 400 S - 400S - 400 M - 500M - 500 P - 500P - 500 S - 500S - 500 M - 600M - 600 P - 600P - 600 S - 600S - 600 Figure S2. Digital images of grey mullet (M), striped prawn (P) and rose shrimp (S) samples treated at 400-, 500- and 600-MPa pressure, compared to the untreated samples. 114 Italian Journal of Food Science, 2023; 35 (3) Pinheiro ACAS et al. 0 20 40 60 80 0 5 10 15 20 25 30 35 F1 F2 F or ce ( N ) Time (s) Figure S3. Results of the analysed hardness (F1) and index of resistance to compression (F2). _Hlk56781082 _Hlk510604571 _Hlk510602300 _Hlk137473821