id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
cord-344979-ngujhhp6	Lübke, Nadine	Extraction-free SARS-CoV-2 detection by rapid RT-qPCR universal for all primary respiratory materials	2020-08-05		.txt	text/plain	3246	120	46	Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-qPCR Rapid direct nucleic acid amplification test without RNA extraction for SARS-CoV-2 using a portable PCR thermocycler Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal 1 specimens without extraction of viral RNA Screening for SARS-CoV-2 infections with colorimetric RT-LAMP and LAMP sequencing Rapid Molecular Detection of SARS-CoV-2 (COVID-19) Virus RNA Using Colorimetric LAMP Rapid SARS-CoV-2 testing in primary material based on a novel multiplex LAMP assay LAMP-Seq: Population-Scale COVID-19 Diagnostics Using Combinatorial Barcoding Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards Predicting infectious SARS-CoV-2 from diagnostic samples Figure 1: Analysis of PCR conditions for the detection of SARS-CoV-2 by direct RT-qPCR Direct RT-qPCR with the Sarbeco and the new CoV-E primers Patient matched respiratory crude samples (n=20) including swabs, tracheal secretion, brochoalveolare lavage and sputum were analyzed for the detection of SARS-CoV-2 by direct RT-qPCR with the Sarbeco primers and the new CoV-E primers Direct RT-qPCR with fresh and pre-frozen PCR-mixes Patient matched respiratory crude samples (n=8) including swabs, tracheal secretion and BAL were analyzed for the detection of SARS-CoV-2 by direct RT-qPCR with either fresh prepared master-mixes (MMX) or pre-frozen master-mixes The authors gratefully acknowledge the TaKaRa™ employees, especially Sascha Deppermann and Matthieu Lewis for continuous scientific and technical support in establishment and optimization of J o u r n a l P r e -p r o o f HBV-SynQ (internal control): a synthetical plasmid coding for an inactivated s-Antigen of Hepatitis B Next, we analyzed if an increase in crude sample material input increases detection of SARS-CoV-2.	cache/cord-344979-ngujhhp6.txt	txt/cord-344979-ngujhhp6.txt
