key: cord-322220-mlphue1r
authors: Bosis, Samantha; Esposito, Susanna; Niesters, Hubert G.M.; Tremolati, Elena; Pas, Susan; Principi, Nicola; Osterhaus, Albert D.M.E.
title: Coronavirus HKU1 in an Italian pre-term infant with bronchiolitis
date: 2007-02-21
journal: J Clin Virol
DOI: 10.1016/j.jcv.2006.11.014
sha: 
doc_id: 322220
cord_uid: mlphue1r

nan

Respiratory syncytial virus (RSV) is the major cause of bronchiolitis, a common disease in the first months of life Lanari et al., 2002; Purcell and Fergie, 2004) . Bronchiolitis is also associated with influenza and parainfluenza viruses, adenovirus, rhinoviruses, and enteroviruses Coiras et al., 2003; Legg et al., 2005) . Although human metapneumovirus (hMPV) and human coronavirus NL63 (HCoV-NL63) have been identified and associated with a significant proportion of bronchiolitis over the last 3 years, no pathogen is recovered in a substantial proportion of cases (Bastien et al., 2005; Chiu et al., 2005; Esper et al., 2005; Principi et al., 2004 Principi et al., , 2006 .

While studying the epidemiology of viral respiratory infections in 2156 children (1190 males; mean age ± S.D., 3.39 ± 3.40 years) who attended the Emergency Department of Milan University's Institute of Pediatrics because of acute disease (58.2% respiratory tract infections, 12.9% gastrointestinal and intra-abdominal diseases, 5.1% fever of unknown origin, 4.5% seizures with or without fever, 4.7% exanthematious disease, 4.4% nephritic or nephrotic syndrome, 3.7% skin and soft tissue infections, 2.3% bone or joint infections, 1.7% coagulation disorders, 1.0% meningitis/encephalitis, 0.9% sepsis, and 0.6% conjunctivitis) during 

A 1.5-month-old premature male Italian infant was admitted to the Emergency Department of Milan University's Institute of Pediatrics in February 2005 with a 2-day history of poor feeding, rhinorrhea, cough, and dyspnea in the absence of fever. He had been spontaneously delivered after a premature membrane rupture at the gestational age of 33 weeks (birth weight 2670 g; Apgar score 9 at 1 min). At birth, he was asymptomatic and no respiratory support was required. He was hospitalised for 7 days before being sent home. He was mixed-fed from birth, and showed adequate growth. He had never left Italy, and none of his contacts had travelled abroad in the previous 6 months. The child's father had experienced an uncomplicated febrile upper respiratory tract infection starting the day before the child was admitted. Bronchiolitis was diagnosed on the basis of the findings of rhinorrhea, tachypnea, chest retractions, wheezing and bibasilar rales. Chest radiography showed hyperinflated lungs and an increased anteroposterior diameter in lateral view without any parenchymal abnormalities. A nasopharyngeal aspirate was tested for adenovirus, influenza virus types A and B, RSV types A and B, parainfluenza viruses types 1, 2, 3 and 4, rhinoviruses, human metapneumovirus, human coronavirus 229E human coronavirus OC43, human coranovirus NL63 and HCoV-HKU1 by real-time amplification assays at the Department of Virology, Erasmus University Medical Center, Rotterdam, The Netherlands Fouchier et al., 2004; Heim et al., 2003; Kares et al., 2004;  Maertzdorf et al., 2004; van der Hoek et al., 2004; Woo et al., 2005a) . Total nucleic acids were isolated using a Magna-PureLC Isolation Station (Roche Applied Science, Penzberg, Germany), and a universal internal control virus to monitor the process from isolation of the nucleic acids until their real-time detection (Niesters, 2002) . The RNA was amplified in a single-tube, two-step reaction using Taqman reverse transcription reagents and a PCR core reagent kit (Applied Biosystems, Nieuwerkerk a/d IJssel, The Netherlands) in an ABI 7700 or ABI 7500 sequence detection system (Applied Biosystems). A cultured virus was used as a positive control for each assay except in the case of HCoV-HKU1, for which a cloned construct was synthesised from published sequences of part of the nucleoprotein gene (Woo et al., 2005a) . On the basis of proficiency testing data (Templeton et al., 2006) , the estimated sensitivity of each assay was less than 500-1000 copies/mL.

In the case of HCoV-HKU1, the NP gene (1326 bp), S gene (4441 bp) and part of the polymerase (ORF1B, 2815 bp) were amplified and sequenced using previously described primers (Woo et al., 2005c) , and the sequence data analysed using a Sequence Navigator software sequencer (Applied Biosystems) and Seqman (DNASTAR, Madison, WI). The sequences were aligned using Clustal W (Bioedit 7.0.1). All of the available sequences of the corresponding gene sequences in Genbank were aligned, and their phylogenetic relations were calculated by means of bootstrapping resampling in order to calculate nodal confidence (n = 1000) using Kamura's two-parameter neighbour-joining method and MEGA 3.1. The data are shown in Fig. 1 .

Only one of 2156 aspirates was positive for HCoV-HKU1, and the low threshold cycle value (16) indicated a high viral load. Therapy consisted of oxygen supplementation, intravenous rehydration, and inhaled bronchodilators. The infant was discharged after 6 days and no respiratory recurrences were observed in the subsequent 6 months.

HCoV-HKU1 is a coronavirus that has been detected in adults with pneumonia in China, and in small groups of children with respiratory or enteric infections in China, Australia, France and the United States (Esper et al., 2006; Lau et al., 2006; Sloots et al., 2006; Vabret et al., 2006; Woo et al., 2005a,b,c) . Complete genome sequence analyses suggests that it originated from one major and some minor recombinations of group 2 coronaviruses . The Milan HCoV-HKU1 is closely related to the original viruses described by Woo et al. (2005a,b) , with minimal changes in the NP, S, and a large part of the polymerase 1B gene fragment. The smaller part of the polymerase 1B fragment discriminates the Milan virus more clearly from the others (Sloots et al., 2006; Woo et al., 2006) , and has a closer relationship with the Hong Kong sequences and is distant from the Australian sequences.

Although the cause-effect relationship between HCoV-HKU1 and the disease of our patient could have only been confirmed by means of a respiratory tissue biopsy or serology on paired sera, it seems likely that this case of bronchiolitis was caused by HCoV-HKU1, which was present in large quantity. This is the second time that bronchiolitis has been associated with this virus, which has never been previously demonstrated in Southern Europe (Esper et al., 2006; Sloots et al., 2006; Vabret et al., 2006; Woo et al., 2005a,b,c) .

Detecting HCoV-HKU1 in the nasopharyngeal secretions of a premature bronchiolitic infant confirms that HCoVs can cause moderate/severe respiratory infections (Fouchier et al., 2005; Kahn and McIntosh, 2005) . As only a few patients with HCoV-HKU1 infection have been described so far, it is impossible to define the real importance of the virus. The fact that the majority of the reported cases (including ours) experienced a lower respiratory infection requiring hospitalisation suggests that its clinical impact may be significant. Previous studies have demonstrated that children aged less than 2 years are most at risk of HCoV-HKU1 infection, which significantly contributes to the microbial burden of patients with respiratory and enteric diseases during the colder months (Esper et al., 2006; Lau et al., 2006; Sloots et al., 2006; Vabret et al., 2006) . HCoV-HKU1 can also be present as a persistent asymptomatic infection in patients with underlying conditions Vabret et al., 2006) . However, further epidemiological and clinical investigations are needed to precisely define the role of HCoV-HKU1 in respiratory infections.

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